CN210675282U - Auxiliary reminding device for micropore plate sample adding - Google Patents

Auxiliary reminding device for micropore plate sample adding Download PDF

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Publication number
CN210675282U
CN210675282U CN201921437195.0U CN201921437195U CN210675282U CN 210675282 U CN210675282 U CN 210675282U CN 201921437195 U CN201921437195 U CN 201921437195U CN 210675282 U CN210675282 U CN 210675282U
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CN
China
Prior art keywords
test tube
holes
sample
led lamp
controller
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Expired - Fee Related
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CN201921437195.0U
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Chinese (zh)
Inventor
陈世东
张翠霞
练明建
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First Affiliated Hospital of Xiamen University
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First Affiliated Hospital of Xiamen University
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Priority to CN201921437195.0U priority Critical patent/CN210675282U/en
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Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a microplate sampling auxiliary reminding device, which comprises a test tube rack and an indicating base; the top surface of the indicating base is matched with a fixed seat used for being placed on a micropore plate, the fixed seat is provided with a plurality of through holes which are in one-to-one correspondence with the reaction holes of the micropore plate, and each through hole is respectively provided with an LED lamp; the indicating base is internally provided with a controller electrically connected with each LED lamp and a control switch electrically connected with the controller; the LED lamp test tube is characterized in that the test tube rack is provided with a plurality of test tube holes for inserting test tubes, the number of the test tube holes is the same as that of the through holes, photosensitive sensors are arranged in the test tube holes respectively, the photosensitive sensors correspond to the LED lamps one to one, and the photosensitive sensors are electrically connected with the controller. The utility model provides a micropore board appear the mistake easily in artifical application of sample in-process and add with the problem of hourglass.

Description

Auxiliary reminding device for micropore plate sample adding
Technical Field
The utility model relates to a pathological examination field especially indicates a supplementary reminding device of micropore board application of sample.
Background
In biological assay detection experiments, microplates are often used as vessels for carrying out reactions, microplates are usually transparent, and microplates have 6-well plates, 12-well plates, 48-well plates, 96-well plates, 384-well plates and 1536-well plates according to the number of reaction wells, wherein the 96-well plates, 354-well plates and 1536-well plates are particularly suitable for high-throughput assay detection, such as enzyme-linked immunosorbent assays (ELISA) and polymerase chain amplification assays (PCR), which are the most basic and widely used in immunology and molecular biology, and microplates are required as vessels for reactions.
At present, two methods are generally used for adding reagents and samples into reaction holes of a micro-porous plate, wherein one method is automatic filling by an instrument, and the other method is manual filling. The filling method is carried out by using an instrument, is suitable for laboratories with large-flux samples, and the instrument is complex in structure and very expensive. At many medical health institutions, research unit, because the sample size is not big, generally all adopt the manual work to come the application of sample and add the reagent, at the application of sample in-process, operating personnel need adopt the pipettor with the sample that awaits measuring in the test tube that holds the sample that awaits measuring transfer to the reaction hole on the micropore board in the one-to-one, and the reaction hole on the micropore board is many and close, make the application of sample process very loaded down with trivial details, it is very easily wrong or neglected loading to have negligence to appear, and then lead to the experimental result to appear the mistake, arouse great accident easily, the disp.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a reminding device is assisted in micropore board application of sample has solved the micropore board and has appeared the mistake easily and add and leak the problem that adds in artifical application of sample in-process.
In order to achieve the above purpose, the solution of the present invention is:
a microplate sample adding auxiliary reminding device comprises a test tube rack and an indicating base; the top surface of the indicating base is matched with a fixed seat used for being placed on a micropore plate, the fixed seat is provided with a plurality of through holes which are in one-to-one correspondence with the reaction holes of the micropore plate, and each through hole is respectively provided with an LED lamp; the indicating base is internally provided with a controller electrically connected with each LED lamp and a control switch electrically connected with the controller; the LED lamp test tube is characterized in that the test tube rack is provided with a plurality of test tube holes for inserting test tubes, the number of the test tube holes is the same as that of the through holes, photosensitive sensors are arranged in the test tube holes respectively, the photosensitive sensors correspond to the LED lamps one to one, and the photosensitive sensors are electrically connected with the controller.
The test tube hole includes cylinder hole and the conical bore of aperture top-down convergent, and the conical bore upper end links up with cylinder hole lower extreme, and photosensitive sensor sets up on the center of conical bore bottom.
The photosensitive sensor is a photosensitive diode, a photosensitive resistor or a photosensitive triode.
After the scheme is adopted, when the utility model is used, the test tube containing the sample to be tested is firstly inserted into the test tube hole of the test tube rack, and the photosensitive sensor in the test tube hole inserted with the test tube is shielded and cannot sense light; meanwhile, the micro-porous plate is placed on the fixed seat of the indicating base, so that each reaction hole of the micro-porous plate is respectively embedded in each through hole of the fixed seat, and each reaction hole of the micro-porous plate corresponds to each LED lamp one by one; then, a control switch is turned on, at the moment, the controller controls the LED lamps to be completely turned off, and the photosensitive sensor which is shielded and cannot sense light is marked as a photosensitive sensor with a sample; thus, when an operator carries out manual sample adding, when the operator firstly takes up a test tube on a test tube rack, the photosensitive sensor with the sample corresponding to the test tube is not shielded, the photosensitive sensor with the sample receives light rays and transmits a signal to the controller, so that the controller controls the LED lamp corresponding to the photosensitive sensor with the sample to flicker, thereby indicating a reaction hole corresponding to the sample to be detected in the test tube on a microporous plate, so that the operator can quickly find the reaction hole corresponding to the sample to be detected in the test tube, then the operator adds the sample to be detected in the test tube to the reaction hole corresponding to the sample to be detected in the test tube through a pipettor, finally the operator inserts the taken test tube back into the original test tube hole, at the moment, the photosensitive sensor with the sample corresponding to the test tube is shielded and cannot feel light rays, and the photosensitive sensor with the sample does not transmit a signal to the controller any more, the controller controls the LED lamp corresponding to the photosensitive sensor with the sample to be normally on, so that the reaction hole corresponding to the normally-on LED lamp is indicated to finish sample adding.
To sum up, the utility model discloses a set up photosensitive sensor in the test tube hole of test-tube rack and set up the LED lamp on instructing the base, make the utility model discloses when the artifical application of sample on the micropore board, can instruct the corresponding micropore board reaction hole of the sample that awaits measuring that will drip with operating personnel through the LED lamp for operating personnel just can find fast and will drip the corresponding micropore board reaction hole of the sample that awaits measuring and carry out the application of sample, and after the application of sample is accomplished, can instruct the micropore board reaction hole of accomplishing the application of sample through the LED lamp, the problem of appearing wrong application of sample and hourglass application of sample when can avoiding the artifical application of sample of operating personnel like this.
Drawings
Fig. 1 is an exploded view of the present invention;
FIG. 2 is a schematic structural view of the present invention;
fig. 3 is a cross-sectional view of the test tube rack of the present invention.
Detailed Description
In order to further explain the technical solution of the present invention, the present invention is explained in detail by the following embodiments.
As shown in fig. 1 to fig. 3, the utility model discloses a supplementary reminding device of micropore board application of sample, it includes test-tube rack 1 and instructs base 2.
Specifically, as shown in fig. 1 and fig. 2, a fixing seat 21 for placing on a micro-porous plate is matched with the top surface of the indicating base 2, a plurality of through holes 211 corresponding to each reaction hole of the micro-porous plate one by one are arranged on the fixing seat 21, and an LED lamp 212 is respectively arranged in each through hole 211; a controller (not shown) electrically connected with each LED lamp 212 and a control switch 22 electrically connected with the controller are installed in the indicating base 2, wherein the controller can adopt a single chip microcomputer, the controller is used for controlling the working state of each LED lamp 212, the controller and each LED lamp can be welded on a circuit board, and a circuit connection is formed through a circuit on the circuit board; the control switch 22 is used for providing a control signal to the controller or controlling whether the controller is powered on or not, and the control switch 22 can be arranged on the top surface of the indication base 2; the controller and the LED lamps 212 can be powered by a battery arranged in the indicating base 2 or an external power supply, and the fixed seat 21 can be arranged on the top surface of the indicating base 2 in a screw locking or clamping manner; in addition, in order to prevent the device from being damaged due to leakage when the sample is dripped on the micropore plate, the top surface of the indication base 2 can be covered with a waterproof film 23.
As shown in fig. 1 to 3, the test tube rack 1 is provided with a plurality of test tube holes 11 for inserting test tubes, the number of the test tube holes 11 is the same as that of the through holes 211, a photosensitive sensor 12 for sensing light is respectively arranged in each test tube hole 11, each photosensitive sensor 12 corresponds to each LED lamp one by one, and each photosensitive sensor 12 is electrically connected with the controller so that each photosensitive sensor 12 can send signals to the controller; wherein each photosensitive sensor 12 can be arranged on a circuit board which is electrically connected with the controller through a lead so that each photosensitive sensor 12 is electrically connected with the controller; and as shown in fig. 3, the test tube hole 11 may include a cylindrical hole 111 and a conical hole 112 with a gradually reduced diameter from top to bottom, the upper end of the conical hole 112 is connected to the lower end of the cylindrical hole 111, the photosensor 12 is disposed in the center of the bottom of the conical hole 112, so that after the test tube containing the sample to be detected is inserted into the test tube hole 11, the bottom of the test tube will abut against the inner wall of the conical hole 112 and completely shield the photosensor 12, so that the photosensor 12 cannot receive light, and the photosensor 12 is a photodiode, a photoresistor or a phototriode.
When the utility model is used, the test tube containing the sample to be tested is firstly inserted into the test tube hole 11 of the test tube rack 1, and the photosensitive sensor 12 in the test tube hole 11 inserted with the test tube is shielded and cannot sense light; meanwhile, the microplate is placed on the fixed seat 21 of the indicating base 2, so that each reaction hole of the microplate is respectively embedded in each through hole 211 of the fixed seat 21, and each reaction hole of the microplate corresponds to each LED lamp 212 one by one; the control switch 22 is then turned on, and the controller first controls the LED lamp 212 to be fully turned off and marks the photosensor 12 that is shielded from sensing light as the photosensor 12 with the sample. Thus, when an operator carries out manual sample adding, when the operator first takes up a test tube on the test tube rack 1, the photosensitive sensor 12 with a sample corresponding to the test tube is not shielded, the photosensitive sensor 12 with the sample receives light and transmits a signal to the controller, so that the controller controls the LED lamp 212 corresponding to the photosensitive sensor 12 with the sample to flash, thereby indicating the reaction hole corresponding to the sample to be detected in the test tube on the microporous plate, so that the operator can quickly find the reaction hole corresponding to the sample to be detected in the test tube, then the operator adds the sample to be detected in the test tube to the reaction hole corresponding to the sample to be detected in the test tube through a pipettor, finally, the operator inserts the taken test tube back into the original test tube hole 11, and the photosensitive sensor 12 with the sample corresponding to the test tube is shielded and cannot sense light, the photosensor 12 with the sample no longer transmits a signal to the controller, so that the controller controls the LED lamp 212 corresponding to the photosensor 12 with the sample to be normally on, thereby indicating the reaction well corresponding to the normally on LED lamp to complete sample application.
In conclusion, the utility model discloses a set up photosensitive sensor 12 in test tube hole 11 of test-tube rack 1 and set up LED lamp 212 on instructing base 2, make the utility model discloses when the artifical application of sample on the micropore board, can instruct the corresponding micropore board reaction hole of the sample that awaits measuring that will drip with operating personnel through LED lamp 212 for operating personnel just can find fast and will drip the corresponding micropore board reaction hole of the sample that awaits measuring and carry out the application of sample, and after the application of sample is accomplished, can instruct the micropore board reaction hole of accomplishing the application of sample through LED lamp 212, the problem of appearing wrong application of sample and hourglass application of sample when can avoiding the artifical application of sample of operating personnel like this.
The above embodiments and drawings are not intended to limit the form and style of the present invention, and any suitable changes or modifications made by those skilled in the art should not be construed as departing from the scope of the present invention.

Claims (3)

1. The utility model provides a supplementary reminding device of micropore board application of sample which characterized in that: comprises a test tube rack and an indicating base;
the top surface of the indicating base is matched with a fixed seat used for being placed on a micropore plate, the fixed seat is provided with a plurality of through holes which are in one-to-one correspondence with the reaction holes of the micropore plate, and each through hole is respectively provided with an LED lamp; the indicating base is internally provided with a controller electrically connected with each LED lamp and a control switch electrically connected with the controller;
the LED lamp test tube is characterized in that the test tube rack is provided with a plurality of test tube holes for inserting test tubes, the number of the test tube holes is the same as that of the through holes, photosensitive sensors are arranged in the test tube holes respectively, the photosensitive sensors correspond to the LED lamps one to one, and the photosensitive sensors are electrically connected with the controller.
2. The microplate sample addition auxiliary reminding device of claim 1, wherein: the test tube hole includes cylinder hole and the conical bore of aperture top-down convergent, and the conical bore upper end links up with cylinder hole lower extreme, and photosensitive sensor sets up on the center of conical bore bottom.
3. The microplate sample addition auxiliary reminding device of claim 1 or 2, wherein: the photosensitive sensor is a photosensitive diode, a photosensitive resistor or a photosensitive triode.
CN201921437195.0U 2019-08-30 2019-08-30 Auxiliary reminding device for micropore plate sample adding Expired - Fee Related CN210675282U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921437195.0U CN210675282U (en) 2019-08-30 2019-08-30 Auxiliary reminding device for micropore plate sample adding

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921437195.0U CN210675282U (en) 2019-08-30 2019-08-30 Auxiliary reminding device for micropore plate sample adding

Publications (1)

Publication Number Publication Date
CN210675282U true CN210675282U (en) 2020-06-05

Family

ID=70898828

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921437195.0U Expired - Fee Related CN210675282U (en) 2019-08-30 2019-08-30 Auxiliary reminding device for micropore plate sample adding

Country Status (1)

Country Link
CN (1) CN210675282U (en)

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20200605

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CF01 Termination of patent right due to non-payment of annual fee