CN210596099U - Follow-on cell culture perfusion device - Google Patents
Follow-on cell culture perfusion device Download PDFInfo
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- CN210596099U CN210596099U CN201921548243.3U CN201921548243U CN210596099U CN 210596099 U CN210596099 U CN 210596099U CN 201921548243 U CN201921548243 U CN 201921548243U CN 210596099 U CN210596099 U CN 210596099U
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Abstract
The utility model provides an improved cell culture perfusion device, which comprises an incubator, a heating pipe, a nutrient solution perfusion box structure for stirring, a protective nutrient solution injection pipe structure for adjusting, a cell culture sampling cup structure, a mounting plate, a stirring control switch, a heating control switch, a liquid inlet pipe, a liquid inlet box, a sealing plate, a handle, a flow guide pipe, a liquid discharge pipe, a valve and a liquid discharge pipe, wherein the heating pipe is connected with the lower part of the inner side of the incubator by bolts; the stirring is for nutrient solution perfusion case structure setting in the upper portion of incubator. The utility model discloses the setting of connecting plate, nutrient solution storage box, motor, puddler, stirring board, protective tube, sealed lid, conveyer pipe, protecting crust, discharge pipe, separation net and discharge cover is favorable to stirring the nutrient solution of culture cell; the setting of injection syringe, sleeve pipe, control tube, stationary blade, bell and spigot joint pipe and communicating pipe is favorable to increasing the cultivation effect of cell.
Description
Technical Field
The utility model belongs to the technical field of cell culture, especially, relate to an improved generation cell culture perfusion device.
Background
The stem cells are multipotential cells with self-replication ability, can be differentiated into various functional cells under certain conditions, are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are divided into three categories according to the development potentiality of the stem cells; the stem cell is an insufficiently differentiated and immature cell, has the potential function of regenerating various tissues, organs and human bodies, is called as universal cell in the medical field, and generally consists of a plurality of culture dishes and flasks at present.
The Chinese patent publication No. CN208857308U discloses a stem cell culture perfusion device, which comprises a perfusion bottle, wherein conveying pipes are inserted into two sides of the circumferential outer wall of the perfusion bottle, one ends of the two conveying pipes, which are far away from the perfusion bottle, are connected with a discharge pipe through flanges, the bottom of the discharge pipe is connected with an electromagnetic valve through flanges, mounting shells are arranged on two sides of the outer wall of the top of the perfusion bottle, first electric telescopic rods are arranged in the middle positions of the inner walls of the tops of the two mounting shells, one end, which is far away from the mounting shells, of the two first electric telescopic rods is provided with a same water outlet pipe, the bottom of the circumferential outer wall of the water outlet pipe is connected with nozzles which are distributed at equal intervals through flanges, scrapers are arranged at the tops of the circumferential outer walls of the water outlet pipes, a transition pipe is arranged in the middle position of the inner wall of the top of the perfusion bottle, the, and the bottom inner wall of box is equipped with the heating resistor silk, the intermediate position of box top outer wall is equipped with agitator motor, and the bottom of box one side outer wall is equipped with the delivery pump, one side of perfusion bottle bottom outer wall is equipped with two extension rods, and the bottom of two extension rod one sides is equipped with same mounting panel. However, the existing cell culture perfusion device has the problems that the nutrient solution for culturing the cells is not convenient to stir, the culture effect of the cell culture device is single, and the cultured cells are not convenient to sample.
Therefore, it is necessary to develop an improved cell culture perfusion apparatus.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides an improved generation cell culture perfusion device to solve the inconvenient nutrient solution to cultivateing the cell of current cell culture perfusion device and stir, cell culture device culture effect is single and inconvenient carries out the problem of taking a sample to the cell of cultivateing. An improved cell culture perfusion device comprises an incubator, a heating pipe, a stirring nutrient solution perfusion box structure, an adjusting and protecting nutrient solution injection pipe structure, a cell culture sampling cup structure, a mounting plate, a stirring control switch, a heating control switch, a liquid inlet pipe, a liquid inlet box, a sealing plate, a handle, a flow guide pipe, a liquid discharge pipe, a valve and a liquid discharge pipe, wherein the heating pipe is in bolted connection with the lower part of the inner side of the incubator; the stirring nutrient solution perfusion box structure is arranged at the upper part of the incubator; the adjusting and protecting nutrient solution injection pipe structure is arranged at the upper part of the left side of the incubator; the cell culture sampling cup structure is arranged at the lower part of the right side of the front surface of the incubator; the mounting plate is connected to the upper part of the left side of the front surface of the incubator through a bolt; the stirring control switch and the heating control switch are sequentially embedded in the middle of the front surface of the mounting plate from left to right; the liquid inlet pipe is connected to the lower part of the right side of the incubator through a bolt; the liquid inlet box is connected to the upper part of the liquid inlet pipe through a bolt; the sealing plate is buckled on the upper part of the liquid inlet box; the handle screw is connected to the middle position of the upper surface of the sealing plate; the honeycomb duct is connected to the lower part of the outer surface of the incubator through bolts; the liquid discharge pipe is in threaded connection with the middle position of the bottom of the flow guide pipe; the valve is in threaded connection with the lower part of the liquid discharge pipe; the liquid outlet pipe is in threaded connection with the lower part of the valve; the structure of the nutrient solution filling box for stirring comprises a connecting plate, a nutrient solution storage box, a motor, a stirring rod, a stirring plate, a protective pipe, a sealing cover, a conveying pipe, a protective shell, a discharging pipe, a separation net and a discharging cover, wherein the connecting plate, the nutrient solution storage box, the motor, the stirring rod, the stirring plate, the protective pipe, the sealing cover, the conveying pipe, the protective shell, the discharging pipe, the separation net and the discharging cover are arranged on the left side of the upper surface of the connecting plate in a bolted connection mode; the motor is connected to the middle position of the upper surface of the nutrient solution storage box through a bolt; the stirring rod coupling is connected to an output shaft of the motor; the stirring plate bolt connecting block is arranged at the lower part of the stirring rod; the protective pipe is in threaded connection with the right side of the upper surface of the nutrient solution storage box; the upper part of the sealing cover; the conveying pipe is in threaded connection with the lower part of the right side of the nutrient solution storage box; the protective shell is in threaded connection with the right side of the conveying pipe; the discharge pipe is in threaded connection with the right side of the protective shell; the isolation net is connected to the lower part of the discharge pipe in a threaded manner; the exhaust hood is connected to the middle of the bottom of the isolation net through bolts.
Preferably, the incubator specifically adopts a stainless steel box; the inner wall of the incubator is glued with a heat preservation sponge layer.
Preferably, the inner diameter of the flow guide pipe is set to be twenty millimeters to forty millimeters; the honeycomb duct specifically adopts the rubber hose.
Preferably, the nutrient solution storage box is a stainless steel box; an acrylic transparent plastic plate is embedded in the middle of the front surface of the nutrient solution storage box.
Preferably, the protective shell is a stainless steel shell with an arc-shaped top; and a stainless steel net is connected with the middle position of the inside of the protective shell through a screw.
Preferably, the adjusting and protecting nutrient solution injection pipe structure comprises an injection pipe, a sleeve, an adjusting pipe, a fixing piece, a sleeve pipe and a communicating pipe, wherein the sleeve pipe is connected to the upper part of the injection pipe through a bolt; the adjusting pipe is in threaded connection with the upper part of the sleeve; the fixing piece is glued on the upper part of the right side of the front surface of the adjusting pipe; the sleeve pipe is in threaded connection with the upper part of the right side of the adjusting pipe; the communicating pipe is in threaded connection with the right side of the sleeve pipe.
Preferably, the injection tube is communicated with the sleeve; the sleeve is communicated with the adjusting pipe; the adjusting pipe is communicated with the sleeve pipe; the sleeve pipe is communicated with the communicating pipe.
Preferably, the adjusting pipe is a stainless steel hose.
Preferably, the cell culture sampling cup structure comprises a protective sheet, a cell sampling storage cup, a grab handle, an insertion tube, a suction tube and a marking sheet, wherein the cell sampling storage cup is connected with the lower part of the right side of the front surface of the protective sheet through a bolt; the grab handle is connected with the middle position on the left side of the cell sampling storage cup through a bolt; the inserting pipe is connected to the lower part of the left side of the front surface of the protection sheet through a bolt; the suction pipe is longitudinally inserted at the upper part of the inner side of the insertion pipe; the marking sheet is glued at the middle position of the upper part of the front surface of the protection sheet.
Preferably, the cell sampling storage cup is an acrylic transparent plastic cup.
Preferably, the marking sheet is a rubber sheet.
Preferably, the connecting plate is connected with the incubator through bolts; the communicating pipe is in threaded connection with the incubator; the protection sheet is connected with the incubator through bolts.
Preferably, the stirring control switch is electrically connected with the motor; the heating control switch is electrically connected with the heating pipe.
Preferably, the stirring control switch and the heating control switch are switches with the model number of SB16 respectively; the motor is specifically a motor with the model number YL; the heating pipe is particularly a heating pipe with the model number of DN 40.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses in, connecting plate, nutrient solution storage box, motor, puddler stir the board, the protecting tube seals the lid, the conveyer pipe, the protecting crust, the delivery pipe, the setting of separation net and discharge cover is favorable to stirring the nutrient solution of cultivateing the cell.
2. The utility model discloses in, the syringe, the sleeve pipe, the control tube, the stationary blade, the setting of cup jointing pipe and communicating pipe is favorable to increasing the cultivation effect of cell.
3. The utility model discloses in, the protection piece, cup, grab handle, the pipe of pegging graft, the setting of straw and mark piece are favorable to taking a sample to the cell of cultivateing.
4. In the utility model, the incubator is a stainless steel box; the inner wall of the incubator is glued with a heat preservation sponge layer, which is beneficial to preserving the heat of the cells in the incubator.
5. In the utility model, the inner diameter of the flow guide pipe is set to be twenty-four millimeters; the honeycomb duct specifically adopt rubber hose, be favorable to adjusting the angle of honeycomb duct.
6. In the utility model, the nutrient solution storage box is a stainless steel box; the middle position on the positive surface of nutrient solution storage box inlay and have acrylic transparent plastic board, be favorable to preventing that the nutrient solution storage box from rustting and damaging.
7. In the utility model, the protective shell is a stainless steel shell with an arc top; the screw in the middle of the protective shell is connected with a stainless steel mesh, which is beneficial to isolating sundries in the nutrient solution.
8. In the utility model, the injection tube is communicated with the sleeve; the sleeve is communicated with the adjusting pipe; the adjusting pipe is communicated with the sleeve pipe; the sleeve pipe is communicated with the communicating pipe, which is beneficial to injecting oxygen in the injection pipe.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the structure of the nutrient solution filling tank for stirring according to the present invention.
Fig. 3 is a schematic structural diagram of the structure of the injection pipe for adjusting and protecting nutrient solution of the present invention.
FIG. 4 is a schematic structural diagram of the cell culture sampling cup structure of the present invention.
In the figure:
1. an incubator; 2. heating a tube; 3. stirring and filling the nutrient solution into the box structure; 31. a connecting plate; 32. a nutrient solution storage box; 33. a motor; 34. a stirring rod; 35. a stirring plate; 36. a protective tube; 37. a sealing cover; 38. a delivery pipe; 39. a protective shell; 310. a discharge pipe; 311. an isolation net; 312. an exhaust hood; 4. adjusting the structure of the protective nutrient solution injection pipe; 41. an injection tube; 42. a sleeve; 43. an adjusting tube; 44. a fixing sheet; 45. sleeving a pipe; 46. a communicating pipe; 5. a cell culture sampling cup structure; 51. a protective sheet; 52. a cell sampling storage cup; 53. a handle; 54. inserting a pipe; 55. a straw; 56. a marking sheet; 6. mounting a plate; 7. a stirring control switch; 8. a heating control switch; 9. a liquid inlet pipe; 10. a liquid inlet tank; 11. a sealing plate; 12. a grip; 13. a flow guide pipe; 14. a liquid discharge pipe; 15. a valve; 16. a liquid outlet pipe.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
as shown in figures 1 to 4
The utility model provides an improved cell culture perfusion device, which comprises an incubator 1, a heating pipe 2, a nutrient solution perfusion box structure 3 for stirring, a regulation and protection nutrient solution injection pipe structure 4, a cell culture sampling cup structure 5, a mounting plate 6, a stirring control switch 7, a heating control switch 8, a liquid inlet pipe 9, a liquid inlet box 10, a sealing plate 11, a handle 12, a flow guide pipe 13, a liquid discharge pipe 14, a valve 15 and a liquid discharge pipe 16, wherein the heating pipe 2 is connected with the lower part of the inner side of the incubator 1 through bolts; the stirring nutrient solution perfusion box structure 3 is arranged at the upper part of the incubator 1; the adjusting and protecting nutrient solution injection pipe structure 4 is arranged at the upper part of the left side of the incubator 1; the cell culture sampling cup structure 5 is arranged at the lower part of the right side of the front surface of the incubator 1; the mounting plate 6 is connected to the upper part of the left side of the front surface of the incubator 1 through a bolt; the stirring control switch 7 and the heating control switch 8 are sequentially embedded in the middle of the front surface of the mounting plate 6 from left to right; the liquid inlet pipe 9 is connected with the lower part of the right side of the incubator 1 through a bolt; the liquid inlet box 10 is connected to the upper part of the liquid inlet pipe 9 through a bolt; the sealing plate 11 is buckled on the upper part of the liquid inlet box 10; the handle 12 is screwed in the middle of the upper surface of the sealing plate 11; the draft tube 13 is connected with the lower part of the outer surface of the incubator 1 through bolts; the liquid discharge pipe 14 is in threaded connection with the middle position of the bottom of the guide pipe 13; the valve 15 is in threaded connection with the lower part of the liquid discharge pipe 14; the liquid outlet pipe 16 is in threaded connection with the lower part of the valve 15; the stirring nutrient solution filling box structure 3 comprises a connecting plate 31, a nutrient solution storage box 32, a motor 33, a stirring rod 34, a stirring plate 35, a protective pipe 36, a sealing cover 37, a conveying pipe 38, a protective shell 39, a discharge pipe 310, a separation net 311 and a discharge cover 312, wherein the connecting plate 31, the nutrient solution storage box 32, the motor 33, the stirring rod 34, the stirring plate 35, the protective pipe 36, the sealing cover 37, the conveying pipe 38, the protective shell 39, the discharge pipe 310, the separation net 311 and the discharge cover 312 are connected with each other through bolts, and the nutrient solution storage box 32 is arranged on the left side of the upper surface of the connecting plate 31 in a bolted connection mode; the motor 33 is connected to the middle position of the upper surface of the nutrient solution storage box 32 through a bolt; the stirring rod 34 is connected to the output shaft of the motor 33 through a coupling; the stirring plate 35 is connected with the lower part of the stirring rod 34 through a bolt; the protective pipe 36 is connected to the right side of the upper surface of the nutrient solution storage box 32 in a threaded manner; the upper part of the sealing cover 37; the conveying pipe 38 is in threaded connection with the lower part of the right side of the nutrient solution storage box 32; the protective shell 39 is connected to the right side of the delivery pipe 38 in a threaded manner; the discharge pipe 310 is screwed on the right side of the protective shell 39; the separation net 311 is in threaded connection with the lower part of the discharge pipe 310; the exhaust hood 312 is bolted to the middle of the bottom of the isolation net 311.
In the above embodiment, specifically, the incubator 1 specifically adopts a stainless steel box; the inner wall of the incubator 1 is glued and connected with a heat preservation sponge layer.
In the above embodiment, specifically, the inner diameter of the flow guide pipe 13 is set to be twenty mm to forty mm; the draft tube 13 is specifically a rubber hose.
In the above embodiment, specifically, the nutrient solution storage tank 32 is a stainless steel tank; an acrylic transparent plastic plate is embedded in the middle of the front surface of the nutrient solution storage box 32.
In the above embodiment, specifically, the protective shell 39 is a stainless steel shell with an arc top; the middle position inside the protective shell 39 is connected with a stainless steel net through a screw.
In the above embodiment, specifically, the adjusting and protecting nutrient solution injection tube structure 4 includes an injection tube 41, a sleeve 42, an adjusting tube 43, a fixing plate 44, a sleeve 45 and a communicating tube 46, wherein the sleeve 42 is bolted to the upper part of the injection tube 41; the adjusting pipe 43 is in threaded connection with the upper part of the sleeve 42; the fixing piece 44 is glued on the upper part of the right side of the front surface of the adjusting pipe 43; the sleeve pipe 45 is in threaded connection with the upper part of the right side of the adjusting pipe 43; the communicating pipe 46 is screwed on the right side of the sleeve pipe 45.
In the above embodiment, specifically, the injection tube 41 is communicated with the sleeve 42; the sleeve 42 is communicated with the adjusting pipe 43; the adjusting pipe 43 is communicated with the sleeve pipe 45; the sleeve pipe 45 is communicated with the communicating pipe 46.
In the above embodiment, specifically, the adjusting tube 43 is a stainless steel hose.
In the above embodiment, specifically, the cell culture sampling cup structure 5 includes a protection sheet 51, a cell sampling storage cup 52, a handle 53, a plugging tube 54, a suction tube 55 and a marking sheet 56, wherein the bolt connection block of the cell sampling storage cup 52 is arranged at the lower part of the right side of the front surface of the protection sheet 51; the grab handle 53 is bolted at the middle position on the left side of the cell sampling and storing cup 52; the inserting pipe 54 is connected to the lower part of the left side of the front surface of the protection sheet 51 through a bolt; the suction pipe 55 is longitudinally inserted at the upper part of the inner side of the insertion pipe 54; the marking plate 56 is glued to the upper middle part of the front surface of the protection plate 51.
In the above embodiment, the cell sampling and storing cup 52 is specifically an acrylic transparent plastic cup.
In the above embodiment, specifically, the marking sheet 56 is a rubber sheet.
In the above embodiment, specifically, the connecting plate 31 is connected to the incubator 1 by bolts; the communicating pipe 46 is in threaded connection with the incubator 1; the protection sheet 51 is connected with the incubator 1 through bolts.
In the above embodiment, specifically, the stirring control switch 7 is electrically connected to the motor 33; the heating control switch 8 is electrically connected with the heating pipe 2.
In the above embodiment, specifically, the stirring control switch 7 and the heating control switch 8 are switches of type SB16 respectively; the motor 33 is a motor with a model number YL; the heating pipe 2 is a heating pipe with model number DN 40.
Principle of operation
When the utility model is used, firstly, the perfusion device is moved to the position of the needed culture device, then the sealing plate 11 is opened by hand, then the cells to be cultured are put into the culture box 1 through the liquid inlet box 10, then the external power supply of the culture device is switched on, then the heating control switch 8 is pressed to start the heating pipe 2, the cells in the culture box 1 can be heated and insulated, then the sealing cover 37 is opened, then the nutrient solution is poured into the nutrient solution storage box 32, then the stirring control switch 7 is pressed to start the motor 33 to drive the stirring plate 35 under the stirring rod 34 to rotate, the nutrient solution in the nutrient solution storage box 32 can be stirred, then the cells cultured in the culture box 1 can be injected with the discharging cover 312, then the oxygen of the cultured cells is sucked into the injection pipe 41, then the oxygen in the injection pipe 41 is injected into the culture box 1, the oxygen in the culture box 1 can be injected with the cultured cells, after the cell culture is completed, the cultured cells can be sampled by using the cell sampling and storing cup 52 in cooperation with the pipette 55.
Utilize technical scheme, or technical personnel in the field are in the utility model discloses under technical scheme's the inspiration, design similar technical scheme, and reach above-mentioned technological effect, all fall into the utility model discloses a protection scope.
Claims (10)
1. The improved cell culture perfusion device is characterized by comprising an incubator (1), a heating pipe (2), a nutrient solution perfusion box structure (3) for stirring, a protective nutrient solution injection pipe structure (4) for adjusting, a cell culture sampling cup structure (5), a mounting plate (6), a stirring control switch (7), a heating control switch (8), a liquid inlet pipe (9), a liquid inlet box (10), a sealing plate (11), a handle (12), a flow guide pipe (13), a liquid discharge pipe (14), a valve (15) and a liquid discharge pipe (16), wherein the heating pipe (2) is in bolted connection with the lower part of the inner side of the incubator (1); the nutrient solution perfusion box structure (3) for stirring is arranged at the upper part of the incubator (1); the adjusting and protecting nutrient solution injection pipe structure (4) is arranged at the upper part of the left side of the incubator (1); the cell culture sampling cup structure (5) is arranged at the lower part of the right side of the front surface of the incubator (1); the mounting plate (6) is connected to the upper part of the left side of the front surface of the incubator (1) through bolts; the stirring control switch (7) and the heating control switch (8) are sequentially embedded in the middle of the front surface of the mounting plate (6) from left to right; the liquid inlet pipe (9) is connected to the lower part of the right side of the incubator (1) through a bolt; the liquid inlet box (10) is connected to the upper part of the liquid inlet pipe (9) through a bolt; the sealing plate (11) is buckled on the upper part of the liquid inlet box (10); the handle (12) is connected to the middle position of the upper surface of the sealing plate (11) through a screw; the honeycomb duct (13) is connected with the lower part of the outer surface of the incubator (1) through bolts; the liquid discharge pipe (14) is in threaded connection with the middle position of the bottom of the guide pipe (13); the valve (15) is in threaded connection with the lower part of the liquid discharge pipe (14); the liquid outlet pipe (16) is in threaded connection with the lower part of the valve (15); the stirring nutrient solution filling box structure (3) comprises a connecting plate (31), a nutrient solution storage box (32), a motor (33), a stirring rod (34), a stirring plate (35), a protective pipe (36), a sealing cover (37), a conveying pipe (38), a protective shell (39), a discharge pipe (310), a separation net (311) and a discharge cover (312), wherein the connecting plate (31), the nutrient solution storage box (32), the motor (33), the stirring rod (34), the stirring plate (35), the protective pipe (36), the sealing cover (37), the conveying pipe (38), the protective shell (39), the discharge pipe (310), the separation net (311) and the discharge cover (312) are connected through bolts, and the nutrient solution storage box (32) is arranged on the left side of the upper surface of the connecting plate (31); the motor (33) is connected to the middle position of the upper surface of the nutrient solution storage box (32) through a bolt; the shaft coupling of the stirring rod (34) is connected to the output shaft of the motor (33); the bolt connecting block of the stirring plate (35) is arranged at the lower part of the stirring rod (34); the protective pipe (36) is in threaded connection with the right side of the upper surface of the nutrient solution storage box (32); the upper part of the sealing cover (37); the conveying pipe (38) is in threaded connection with the lower part of the right side of the nutrient solution storage box (32); the protective shell (39) is in threaded connection with the right side of the conveying pipe (38); the discharge pipe (310) is in threaded connection with the right side of the protective shell (39); the isolation net (311) is in threaded connection with the lower part of the discharge pipe (310); the exhaust hood (312) is connected to the middle position of the bottom of the isolation net (311) through bolts.
2. An improved cell culture perfusion device according to claim 1, wherein the incubator (1) is a stainless steel chamber; the inner wall of the incubator (1) is glued and connected with a heat preservation sponge layer.
3. An improved cell culture perfusion apparatus as claimed in claim 1, wherein the inner diameter of the flow conduit (13) is arranged to be twenty to forty mm; the guide pipe (13) is a rubber hose.
4. An improved cell culture perfusion apparatus as claimed in claim 1, wherein the nutrient solution storage tank (32) is a stainless steel tank; an acrylic transparent plastic plate is embedded in the middle of the front surface of the nutrient solution storage box (32).
5. An improved cell culture perfusion apparatus as claimed in claim 1, wherein the protective housing (39) is a stainless steel housing with an arc top; the middle position in the protective shell (39) is connected with a stainless steel net through a screw.
6. The improved cell culture perfusion apparatus according to claim 1, wherein the adjusting and protecting nutrient solution injection tube structure (4) comprises an injection tube (41), a sleeve (42), an adjusting tube (43), a fixing plate (44), a sleeve (45) and a communicating tube (46), wherein the sleeve (42) is bolted to the upper part of the injection tube (41); the adjusting pipe (43) is in threaded connection with the upper part of the sleeve (42); the fixing sheet (44) is glued on the upper part of the right side of the front surface of the adjusting pipe (43); the sleeve pipe (45) is in threaded connection with the upper part of the right side of the adjusting pipe (43); the communicating pipe (46) is in threaded connection with the right side of the sleeve pipe (45).
7. An improved cell culture perfusion apparatus as claimed in claim 6, wherein the injection tube (41) is in communication with a cannula (42); the sleeve (42) is communicated with the adjusting pipe (43); the adjusting pipe (43) is communicated with the sleeve pipe (45); the sleeve joint pipe (45) is communicated with the communicating pipe (46).
8. An improved cell culture perfusion device according to claim 6, wherein the regulating tube (43) is a stainless steel hose.
9. The improved cell culture perfusion apparatus as claimed in claim 1, wherein the cell culture sampling cup structure (5) comprises a protection sheet (51), a cell sampling storage cup (52), a handle (53), a plug tube (54), a suction tube (55) and a marking sheet (56), wherein the cell sampling storage cup (52) is bolted to the lower part of the right side of the front surface of the protection sheet (51); the grab handle (53) is connected to the middle position on the left side of the cell sampling and storing cup (52) through a bolt; the inserting pipe (54) is connected to the lower part of the left side of the front surface of the protection sheet (51) through a bolt; the suction pipe (55) is longitudinally inserted at the upper part of the inner side of the insertion pipe (54); the marking sheet (56) is glued at the middle position of the upper part of the front surface of the protective sheet (51).
10. An improved cell culture perfusion device according to claim 9, wherein the cell sampling and storing cup (52) is an acrylic transparent plastic cup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921548243.3U CN210596099U (en) | 2019-09-18 | 2019-09-18 | Follow-on cell culture perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921548243.3U CN210596099U (en) | 2019-09-18 | 2019-09-18 | Follow-on cell culture perfusion device |
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| CN210596099U true CN210596099U (en) | 2020-05-22 |
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| CN201921548243.3U Active CN210596099U (en) | 2019-09-18 | 2019-09-18 | Follow-on cell culture perfusion device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812925A (en) * | 2021-01-05 | 2021-05-18 | 管豪 | Microbial fermentation reactor |
| CN114149920A (en) * | 2021-12-08 | 2022-03-08 | 上海睿钰生物科技有限公司 | Perfusion culture device |
| CN114736764A (en) * | 2022-03-30 | 2022-07-12 | 六盘水凉都猕猴桃产业股份有限公司 | Fermentation type kiwi fruit vinegar production device and production process thereof |
-
2019
- 2019-09-18 CN CN201921548243.3U patent/CN210596099U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812925A (en) * | 2021-01-05 | 2021-05-18 | 管豪 | Microbial fermentation reactor |
| CN114149920A (en) * | 2021-12-08 | 2022-03-08 | 上海睿钰生物科技有限公司 | Perfusion culture device |
| CN114736764A (en) * | 2022-03-30 | 2022-07-12 | 六盘水凉都猕猴桃产业股份有限公司 | Fermentation type kiwi fruit vinegar production device and production process thereof |
| CN114736764B (en) * | 2022-03-30 | 2023-12-05 | 六盘水凉都猕猴桃产业股份有限公司 | Fermented kiwi fruit vinegar production device and production process thereof |
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