CN210583781U

CN210583781U - Novel solid phase extraction column

Info

Publication number: CN210583781U
Application number: CN201921553718.8U
Authority: CN
Inventors: 严义勇; 邓炀; 韦家波; 刘自天
Original assignee: SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
Current assignee: SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
Priority date: 2019-09-18
Filing date: 2019-09-18
Publication date: 2020-05-22
Anticipated expiration: 2029-09-18

Abstract

The utility model discloses a novel solid phase extraction post, including the column tube, the inside of column tube is provided with sieve and lower sieve, and it has the filler to fill between two sieves, highly being less than of filler distance between two sieves. The utility model discloses a novel solid phase extraction post, highly being less than of filler distance between two sieve, filler are not pressed from both sides tightly by two sieve, but have certain activity space, make solid phase extraction post improve greatly to the tolerance of granule like this, reduce the degree of difficulty, the flow of sample pretreatment by a wide margin, effectively avoided blocking phenomenon's appearance, effectively improved the work efficiency of solid phase extraction, reduce experiment failure rate. Simultaneously, filling the filler in-process, go up the sieve plate and need not to push down to the surface of packing, can reduce the pressure sensing degree of difficulty through the high control splint position, reduce and lead to the density to differ because of the pressure inequality, and then lead to the problem that experimental result repeatability is not good enough.

Description

Novel solid phase extraction column

Technical Field

The utility model relates to a biochemistry detects technical field, especially relates to a novel solid-phase extraction post.

Background

A Solid Phase Extraction Column (SPE Column, or Solid Phase Extraction Cartridges, SPE Cartridges for short) is a sample pretreatment device developed from a chromatography Column for Extraction, separation, and concentration. The SPE technology is based on a liquid-solid phase chromatography theory, enriches, separates and purifies a sample by adopting selective adsorption and selective elution, and comprises a liquid phase and solid phase physical extraction process; the method can also be approximately regarded as a simple SPE technology of the chromatographic process, which is based on the liquid-solid phase chromatographic theory, adopts the modes of selective adsorption and selective elution to enrich, separate and purify the sample and is a physical extraction process comprising a liquid phase and a solid phase; it can also be viewed approximately as a simple chromatographic process. SPE is a liquid chromatography separation principle using selective adsorption and selective elution. The more common method is to make the liquid sample solution pass through the adsorbent to retain the tested substance, then to select the solvent with proper strength to wash out the impurity, then to use a small amount of solvent to quickly elute the tested substance, thus to achieve the purpose of quick separation, purification and concentration. Interference impurities can be selectively adsorbed, so that the measured substance flows out; or adsorbing impurities and the substance to be detected simultaneously, and then selectively eluting the substance to be detected by using a proper solvent. The application of the solid phase extraction column greatly improves the life of people and is helpful for scientific research and development in agriculture.

CN207913283U discloses a phenyl bonded silica gel solid phase extraction column, it has cavity column tube (1), cavity column tube (1) is provided with upper screen plate (2) and lower screen plate (4) inside it has filler (3) to fill between upper screen plate (2) and lower screen plate (4), the filler is phenyl bonded silica gel. CN207012608U discloses a solid phase extraction post of plasticizing agent analysis is exclusively used in, including the pillar pipe, go into the liquid mouth, liquid outlet and filler, there is a psammitolite filter plate respectively about the filler, there is the top seal on the emulsion mouth, the liquid outlet has the bottom seal, wherein whole solid phase extraction post includes the pillar pipe, go into the liquid mouth, the liquid outlet material is glass material, the solid phase extraction post and the sand core filter plate that adopt glass material have effectively reduced the interference of the plasticizing agent in the original material, and with sealed isolated air at both ends, the practical life of solid phase extraction post has effectively been prolonged, excellent in use effect. CN206167988U discloses a high-efficient solid-phase extraction post, including the cavity cylinder, the upper portion of cavity cylinder is equipped with the inlet, and its bottom is equipped with liquid outlet, its characterized in that: the cavity column is filled with mixed filler formed by mixing target enrichment filler and quartz sand; two sieve plates are arranged in the hollow cylinder, and the mixed filler is arranged between the two sieve plates; the volume of the hollow cylinder is 0.1-100mL, and the filling amount of the mixed filler is 50-2000 mg.

Therefore, in the solid phase extraction column in the prior art, the solid phase extraction filler is compressed through the two sieve plates, the height of the solid phase extraction filler is equal to the distance between the sieve plates, the two sieve plates directly contact with the filler, and then the mobile phase is used for analyzing the column chromatography of the sample on the column for separation and purification. Although the solid phase extraction column greatly facilitates the purification and separation of analytes, the inventors of the present application found in the process of implementing the embodiments of the present application that the solid phase extraction column has at least the following technical problems: (1) the solid phase extraction column has low tolerance to particles, and the particles must be completely removed in the process of analyzing a sample for column chromatography, otherwise, the column blockage phenomenon is caused, and the experiment fails. Therefore, operations such as high-speed centrifugation, filtration and the like are often required in the using process, and the operation is complicated; (2) in the filling process of the solid phase extraction column, the filler needs to be accurately placed in a fine pipeline, and a certain pressure is applied to enable the sieve plate to compress the filler. This brings certain difficulty to production, especially for lower amount of filler, in the production process, often because the pressure is not constant enough, leading to the inconsistent density of column filler, the column chromatography result reproducibility is not good enough; smaller pipelines also impose higher equipment requirements for production packing.

SUMMERY OF THE UTILITY MODEL

In order to solve the defects of the prior art, the utility model provides a novel solid-phase extraction column, aiming at solving the technical problems of difficult loading of the blocking column and the uniform loading of the solid-phase extraction column in the prior art.

The utility model discloses the technical problem that will solve realizes through following technical scheme:

the utility model provides a novel solid phase extraction post, includes the column tube, the inside of column tube is provided with sieve and lower sieve, and it has the filler to fill between two sieves, highly being less than of filler the distance between two sieves.

Furthermore, the upper end opening and the lower end of the column tube are provided with a liquid outlet.

Further, the size of the sieve plate is consistent with the inner diameter of the column tube; the sieve plate is provided with sieve holes.

Further, the pore size of the sieve pore is smaller than the size of the filler.

Further, a piston for the injector is installed in the column tube, the size of the piston corresponds to that of the column tube, and the column tube and the piston form the injector.

Further, the column tube and the two sieve plates are designed integrally.

Furthermore, the column tube and the two sieve plates are designed in a split mode.

The utility model discloses following beneficial effect has:

the utility model discloses a novel solid phase extraction post, highly being less than of filler distance between two sieve, filler are not pressed from both sides tightly by two sieve, but have certain activity space, make solid phase extraction post improve greatly to the tolerance of granule like this, reduce the degree of difficulty, the flow of sample pretreatment by a wide margin, effectively avoided blocking phenomenon's appearance, effectively improved the work efficiency of solid phase extraction, reduce experiment failure rate. Simultaneously, filling the filler in-process, go up the sieve plate and need not to push down to the surface of packing, can reduce the pressure sensing degree of difficulty through the high control splint position, reduce and lead to the density to differ because of the pressure inequality, and then lead to the problem that experimental result repeatability is not good enough.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

In the figure: 1. column tube, 2, upper sieve plate, 3, lower sieve plate, 4, filler, 5, liquid outlet.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the utility model is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element to be referred must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Specifically, the upper sieve plate and the lower sieve plate are arranged from top to bottom in the column tube, and a filler is filled between the upper sieve plate and the lower sieve plate, so that a passing solution can be extracted; the filler is arranged on the lower sieve plate, and a distance h is reserved between the top end of the filler and the lower sieve plate.

The utility model discloses in, right the top of packing with concrete numerical value of distance h between the sieve does not do specifically and restricts down, and it is relevant with the diameter of column tube, actually is relevant with the volume that needs get into the absorbent liquid between two sieves, as long as guarantee to leave certain space between two sieves and form the torrent for liquid, make the sum pack well mixed contact to make the target object in the liquid adsorbed.

The utility model discloses in also not specifically limit to the kind and the use amount of filler, technical personnel in the field can set for according to actual need, as the example, the filler is polystyrene filler.

In the present invention, the material of the column tube is not particularly limited, and preferably, the material of the column tube is a material which is impermeable to water and an organic solvent; or the water-proof and organic solvent-proof material is coated on the surface of the support body.

The utility model discloses in, the upper end opening of pillar pipe, lower extreme are equipped with the liquid outlet.

The size of the sieve plate is consistent with the inner diameter of the column tube; the sieve plate is provided with sieve holes, and the pore diameter of each sieve hole is smaller than the size of the filler.

The utility model discloses in, column casing and two sieve can be the design of integration, also can be for the design of components of a whole that can function independently.

The utility model discloses a theory of operation: adding a lower sieve plate, then adding a filler and then adding an upper sieve plate into the column tube to ensure that a distance is reserved between the upper sieve plate and the filler; the lower part of the column tube is put into a sample to be purified, and a syringe connected with the upper part of the column tube is used for sucking liquid so that the liquid level of the solution is over, and then the solution is pushed out. The sample is purified after being repeated for a plurality of times.

The above-mentioned embodiments only express the embodiments of the present invention, and the description thereof is specific and detailed, but the invention can not be understood as the limitation of the patent scope of the present invention, but all the technical solutions obtained by adopting the equivalent substitution or equivalent transformation should fall within the protection scope of the present invention.

Claims

1. The utility model provides a novel solid phase extraction post, includes the column tube, the inside of column tube is provided with sieve and lower sieve, and it has the filler to fill between two sieves, its characterized in that, highly being less than of filler the distance between two sieves.

2. The novel solid phase extraction column of claim 1, wherein the column tube is open at an upper end and has a liquid outlet at a lower end.

3. The novel solid phase extraction column of claim 1, wherein the size of the sieve plate corresponds to the inner diameter of the column tube; the sieve plate is provided with sieve holes.

4. The novel solid phase extraction column of claim 3, wherein the mesh openings have a pore size smaller than the size of the packing.

5. The novel solid phase extraction column of claim 1, wherein a plunger for a syringe is mounted within the column tube, the plunger corresponding in size to the column tube, the column tube and the plunger forming a syringe.

6. The novel solid phase extraction column of claim 1, wherein the column tube and the two sieve plates are of an integrated design.

7. The novel solid phase extraction column of claim 1, wherein the column tube and the two sieve plates are of a split design.