CN210534086U

CN210534086U - TLC biological self-development isolated plant

Info

Publication number: CN210534086U
Application number: CN201921051918.3U
Authority: CN
Inventors: 李笃信; 闫娜; 徐乃玉; 张真庆
Original assignee: Suzhou University
Current assignee: Suzhou University
Priority date: 2019-07-08
Filing date: 2019-07-08
Publication date: 2020-05-15
Anticipated expiration: 2029-07-08

Abstract

The utility model provides a TLC biological self-developing special device, which comprises a thin layer plate bracket, a connecting device and a scale, wherein the thin layer plate bracket comprises a first cuboid plate, a second cuboid plate, a third cuboid plate and a fourth cuboid plate; the connecting device comprises a connecting device A and a connecting device B; the connecting device A is fixed on the second rectangular plate; the connecting device B is connected with the scale; the connecting device a and the connecting device B are connected to each other. By using the device, the thin-layer plate can be placed on the thin-layer plate support, the position of a sampling point can be positioned by rotating the scale during sampling, and each thin-layer plate is not required to be marked and positioned; moreover, the periphery of each point sample hole on the scale is provided with a mark line, so that the central point of the sample hole can be accurately positioned during sample application, and the problem of spot coincidence after color development is avoided; the device is suitable for samples which need to be unfolded or not after sample application, and has wide applicability.

Description

TLC biological self-development isolated plant

Technical Field

The utility model belongs to the technical field of biology, concretely relates to TLC is biological from developing isolated plant.

Background

Thin layer chromatography-biological autography technology was developed in the last 60 th century as a method for rapidly finding natural active compounds by combining modern chromatographic and biological technologies. The active ingredients can directly show visible spots on a thin-layer plate, and can be directly screened out from crude extracts, so that the method is a method for quickly targeting, tracking, separating and screening the active ingredients under the guidance of activity. The traditional method for screening the active ingredients comprises the steps of firstly separating natural products, determining the chemical structure of each ingredient, then measuring the activity of the natural products, and further screening the active ingredients. The TLC-biological self-development technology can complete the separation, positioning and activity evaluation of the sample at one time, and the separation under the activity guidance can reduce the blindness of compound separation and greatly shorten the separation time of active ingredients. The method has the advantages of simple operation, low consumption, high sensitivity and specificity and the like, is widely applied to separation of natural products and screening of active substances, can be used for screening of natural products with activities of resisting bacteria/fungi, inhibiting cholinesterase, scavenging free radicals, resisting oxidation and the like, and can realize extraction and separation of activity guidance; the minimum inhibitory concentration (MID) of the monomeric active compound can also be measured, and the level of activity of the compound can be estimated by comparing the MID value with that of a control. In addition, the qualitative and quantitative characteristics of TLC biological self-development are utilized, and the antibiotic residue in food, water sample and animal tissue can be monitored. Currently, the research of natural medicinal chemistry has been shifted from a single "compound" mode to a "compound + biological activity" mode, and thus, a rapid, effective and convenient biological activity determination technology has occupied an increasingly important position in natural medicinal chemistry. TLC biological self-development technology has been developed for decades, is a relatively mature technology, can be used together with other activity test methods to accurately evaluate the activity of the compound, and is a biological activity test method which is very suitable for natural pharmaceutical chemistry laboratories because of the advantages of simple operation, high test speed and the like. However, since this method can be used for preliminary screening of biological activity of compounds, in the screening process, there are many kinds of compounds to be screened and dose-dependency of the compounds needs to be examined, which leads to problems of complicated and time-consuming operation. Moreover, the reproducibility is poor due to unstable spotting, and the spot size is difficult to compare; and irregular sample application leads to poor visual effect and even spot superposition.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problems of providing a special TLC biological self-development device to solve the problems of complicated operation and long time consumption in the process of activity screening, poor reproducibility caused by unstable sample application and difficulty in comparing the sizes of spots; and the sample application is irregular, which causes poor visual effect and even spot coincidence.

In order to solve the technical problem, the utility model provides a TLC biology is from developing isolated plant, include: a thin-layer plate bracket, a connecting device and a scale,

the thin plate bracket comprises a first rectangular parallelepiped plate, a second rectangular parallelepiped plate, a third rectangular parallelepiped plate and a fourth rectangular parallelepiped plate, the first rectangular parallelepiped plate is parallel to the fourth rectangular parallelepiped plate, the second rectangular parallelepiped plate is parallel to the third rectangular parallelepiped plate, one end of the second rectangular parallelepiped plate is fixedly connected to the first rectangular parallelepiped plate, the other end of the second rectangular parallelepiped plate is fixedly connected to the fourth rectangular parallelepiped plate, one end of the third rectangular parallelepiped plate is fixedly connected to the first rectangular parallelepiped plate, the other end of the third rectangular parallelepiped plate is fixedly connected to the fourth rectangular parallelepiped plate, the first rectangular parallelepiped plate is perpendicular to the second rectangular parallelepiped plate,

the connecting device comprises a connecting device A and a connecting device B; the connecting device A is fixedly arranged on the second rectangular parallelepiped plate; one end of the connecting device B is fixedly connected with the scale, the other end is movably connected with the connecting device A,

when the thin layer plate is placed on the second rectangular parallelepiped plate, the scale is covered on the thin layer plate.

As a preferable scheme of the special TLC biological self-developing device of the present invention, the length and width of the second cuboid plate are matched with the width and length of the scale, the length of the second cuboid plate is 25-500 mm, and the width of the second cuboid plate is 75-500 mm; the length of scale is 75 ~ 500mm, and the width is 25 ~ 500 mm.

As an optimal selection scheme of TLC biological self-development isolated plant, the scale has 2 ~ 5 rows of some sample holes, and every row contains 5 ~ 20 some sample holes.

As an optimal selection scheme of TLC biological self-development isolated plant, the diameter in some appearance hole is 2 ~ 250mm, and the centre distance between two adjacent some appearance holes is 3 ~ 250 mm.

As the utility model relates to a preferred scheme of TLC biological self-developing isolated plant, be equipped with the mark line around the point appearance hole.

As the utility model relates to a TLC biological self-developing isolated plant's an optimal selection scheme, the material of thin-layer plate support is any one of plastics, timber, stainless steel or PEEK.

As a preferable scheme of the special TLC biological self-developing apparatus of the present invention, the connecting device a is any one of a slide rail, a gear or a key groove; the connecting device B corresponds to the connecting device A, and the connecting device B is any one of a clamping groove, a gear or a flat key.

As a preferred scheme of TLC biological self-development isolated plant, TLC biological self-development isolated plant still includes blast apparatus, blast apparatus sets up the top of first cuboid board or fourth cuboid board.

As a preferred scheme of TLC biology self-development isolated plant, TLC biology self-development isolated plant still includes blast apparatus, blast apparatus sets up in first cuboid board or the fourth cuboid board, blast apparatus's top is less than second cuboid board, the bottom is higher than third cuboid board works as blast apparatus sets up when in the first cuboid board, blast apparatus's the face orientation of blowing fourth cuboid board works as blast apparatus sets up when in the fourth cuboid board, blast apparatus's the face orientation of induced drafting first cuboid board.

Compared with the prior art, the special TLC biological self-development device provided by the utility model has the advantages that the thin-layer plate can be placed on the thin-layer plate bracket, the position of a sampling point can be positioned by rotating the scale during sampling, and each thin-layer plate is not required to be drawn and positioned; moreover, the periphery of each point sample hole on the scale is provided with a mark line, so that the center point of the sample hole can be accurately positioned during sample application, and the problem of spot coincidence after color development is avoided; and the device is suitable for samples which need to be unfolded or not after sample application, and has wide applicability. Therefore, the TLC biological self-developing special device can ensure that the sample application positioning is accurate, the sample application is stable, the operation can be simplified, the repetition is reduced, and the time for positioning and scribing is shortened.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive labor. Wherein the content of the first and second substances,

FIG. 1a is a schematic structural view of a special TLC biological self-development apparatus of the present invention;

FIG. 1b is a schematic side view of the TLC apparatus for biological self-development according to the present invention;

FIG. 1c is a schematic top view of the TLC apparatus for bioautography of the present invention;

FIG. 2 is a schematic structural diagram of a TLC bioautography device of the present invention in example 1, wherein a is a perspective view of the device in use, and b is a schematic diagram after spotting of a thin layer plate;

FIG. 3 is a schematic structural diagram of a TLC bioautography device of the present invention in example 2, wherein a is a perspective view of the device in use, and b is a schematic diagram after spotting of a thin layer plate;

FIG. 4a is a schematic view of the TLC bioautography specialized apparatus of the present invention in example 3;

fig. 4b is a schematic diagram of the TLC autoradiography dedicated device of the present invention in example 4.

Wherein: 1 is a first rectangular parallelepiped plate, 2 is a second rectangular parallelepiped plate, 3 is a third rectangular parallelepiped plate, 4 is a fourth rectangular parallelepiped plate, 5 is a scale, 6 is a connecting device a, 7 is a connecting device B, 8 is a sampling hole, 9 is a thin plate, and 10 is an air blowing device.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the following embodiments.

First, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with at least one implementation of the invention is included. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

Secondly, the utility model discloses utilize the structure schematic diagram etc. to describe in detail, when detailing the embodiment of the utility model, for the convenience of explanation, the schematic diagram that shows a TLC biological self-development isolated plant structure can not do local amplification according to general proportion, moreover the schematic diagram is the example, and it should not limit the scope of the utility model protection here. In addition, the actual fabrication process should include three-dimensional space of length, width and depth.

Example 1

Referring to fig. 1a to 1c, fig. 1a is a schematic view of a special TLC biological self-developing apparatus according to the present invention; FIG. 1b is a schematic side view of the TLC apparatus for biological self-development according to the present invention; fig. 1c is a schematic top view of the special TLC autoradiography apparatus of the present invention. As shown in fig. 1a to 1c, the TLC bioautography dedicated apparatus includes: a thin plate holder (not shown) including a first rectangular parallelepiped plate 1, a second rectangular parallelepiped plate 2, a third rectangular parallelepiped plate 3, and a fourth rectangular parallelepiped plate 4; the connecting device comprises a connecting device A6 and a connecting device B7; the connecting device a6 is provided on the second rectangular parallelepiped plate 2 of the thin-layer plate holder; the connecting device B7 is connected with the scale 5; the connecting means a6 and the connecting means B7 are connected to each other. The second rectangular parallelepiped plate 2 has a length of 205mm and a width of 78 mm; the length of the scale 5 is 78mm, and the width is 25 mm; the connecting device A6 is a slide rail, and the connecting device B7 is a clamping groove; the scale 8 comprises 2 rows of spot holes 8, and each row comprises 5 spot holes 8; the diameter of the spot holes 8 is 10mm, and the center distance of the spot holes 8 is 15 mm. Referring to fig. 2, fig. 2 is a schematic structural diagram of a special TLC autoradiography apparatus of the present invention in embodiment 1, wherein a is a perspective view of the apparatus during use, and b is a schematic diagram after spotting of a thin-layer plate. In this example, the lamella plate size is 75mm 25 mm. When the device is in a use state, the thin layer plate is placed above the second cuboid plate 2, the scale 5 is rotated to enable the scale 5 to cover the thin layer plate, sample application can be directly positioned, and line drawing and positioning on the thin layer plate are not needed. The lamella plates are suitable for spotting non-spread samples.

Example 2

Referring to fig. 3, fig. 3 is a schematic structural view of a special TLC autoradiography apparatus of the present invention in embodiment 2, wherein a is a perspective view of the apparatus during use, and b is a schematic view after spotting of a thin-layer plate. In this example, the rest is the same as example 1 except that: the lamella plates were 75mm by 200mm in size. In the use state, the scale can be placed on the lamella plate by rotating the cover, and the connection device B7 (card slot) can be moved on the connection device a6 (slide rail), so that the sample can be printed on any position of the lamella plate, and the lamella plate can be used to the maximum extent. The lamella plates are suitable for spotting samples that may or may not be spread.

Example 3

Referring to fig. 4a, fig. 4a is a schematic diagram of a special TLC autoradiography apparatus according to the present invention in example 3. In this embodiment, the others are the same as those in embodiment 1 except that the air blowing device 10 is installed on the lamella plate holder. As shown in fig. 4a, a fan may be installed above the fourth rectangular parallelepiped plate 4 of the thin-layer plate holder, and after spotting and developing, the solvent needs to be evaporated, and the fan can accelerate the evaporation of the solvent, thereby shortening the time.

Example 4

Referring to fig. 4b, fig. 4b is a schematic diagram of a special TLC autoradiography apparatus according to the present invention in example 4. In this embodiment, the others are the same as those in embodiment 3 except that a fan is embedded in the fourth rectangular parallelepiped plate 4 of the thin plate holder. After spotting and developing, the accelerated solvent is evaporated by a fan.

It should be understood by those skilled in the art that one of the features or objects of the present invention is to: the utility model provides a TLC biological self-developing special device, the thin layer plate can be placed on the thin layer plate bracket, when sampling, the position of the sampling point can be positioned by rotating the scale, and each thin layer plate is not required to be drawn and positioned; moreover, the periphery of each point sample hole on the scale is provided with a mark line, so that the center point of the sample hole can be accurately positioned during sample application, and the problem of spot coincidence after color development is avoided; and the device is suitable for samples which need to be unfolded or not after sample application, and has wide applicability. Therefore, the TLC biological self-development special device can ensure that the sample application positioning is accurate, the sample application is stable, the operation can be simplified, the repetition is reduced, and the time for positioning and scribing is shortened. The time for positioning and scribing and solvent volatilizing is shortened. Therefore, the TLC biological self-development special device ensures accurate sample application positioning, stable sample application, simplified operation, reduced repetition and shortened positioning and scribing time. The device has great advantages when the thin-layer chromatography-biological self-developing technology is used for rapid target tracking separation and active ingredient screening.

It should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the scope of the claims of the present invention.

Claims

1. A TLC biological self-development special device is characterized by comprising: a thin-layer plate bracket, a connecting device and a scale,

2. A TLC biological self-developing special device as claimed in claim 1, which is characterized in that: the length and the width of the second rectangular solid plate are matched with those of the scale, the length of the second rectangular solid plate is 25-500 mm, and the width of the second rectangular solid plate is 75-500 mm; the length of scale is 75 ~ 500mm, and the width is 25 ~ 500 mm.

3. A TLC biological self-developing special device as claimed in claim 1, which is characterized in that: the scale has 2 ~ 5 rows of point sample holes, and every row contains 5 ~ 20 point sample holes.

4. A TLC biological self-developing special device as claimed in claim 3, which is characterized in that: the diameter of the point sample holes is 2-250 mm, and the center distance between every two adjacent point sample holes is 3-250 mm.

5. A TLC biological self-developing special device as claimed in claim 3, which is characterized in that: and marking lines are arranged around the sample dispensing holes.

6. A TLC biological self-developing special device as claimed in claim 1, which is characterized in that: the thin-layer plate support is made of any one of plastic, wood, stainless steel or PEEK.

7. A TLC biological self-developing special device as claimed in claim 1, which is characterized in that: the connecting device A is any one of a slide rail, a gear or a key groove; the connecting device B corresponds to the connecting device A, and the connecting device B is any one of a clamping groove, a gear or a flat key.

8. A TLC biological self-developing special device as claimed in claim 1, which is characterized in that: the TLC biological self-development special device further comprises an air blowing device, and the air blowing device is arranged above the first cuboid plate or the fourth cuboid plate.

9. A TLC biological self-developing special device as claimed in claim 1, which is characterized in that: the TLC biology self-development special device further comprises an air blowing device, the air blowing device is arranged in the first cuboid plate or/and the fourth cuboid plate, the top of the air blowing device is lower than the second cuboid plate, the bottom of the air blowing device is higher than the third cuboid plate, when the air blowing device is arranged in the first cuboid plate, the air blowing surface of the air blowing device faces the fourth cuboid plate, and when the air blowing device is arranged in the fourth cuboid plate, the air suction surface of the air blowing device faces the first cuboid plate.