CN210533960U - Tensile testing device for biological sample - Google Patents
Tensile testing device for biological sample Download PDFInfo
- Publication number
- CN210533960U CN210533960U CN201921133400.4U CN201921133400U CN210533960U CN 210533960 U CN210533960 U CN 210533960U CN 201921133400 U CN201921133400 U CN 201921133400U CN 210533960 U CN210533960 U CN 210533960U
- Authority
- CN
- China
- Prior art keywords
- clamp
- movable
- substrate
- vertical
- fixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
- 238000009864 tensile test Methods 0.000 title claims abstract description 13
- 239000012472 biological sample Substances 0.000 title claims abstract description 8
- 239000000758 substrate Substances 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 238000003860 storage Methods 0.000 claims abstract description 24
- 238000006073 displacement reaction Methods 0.000 claims abstract description 19
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 8
- 239000010936 titanium Substances 0.000 claims description 8
- 229910052719 titanium Inorganic materials 0.000 claims description 8
- 230000033001 locomotion Effects 0.000 claims description 6
- 230000001681 protective effect Effects 0.000 claims description 5
- 239000004809 Teflon Substances 0.000 claims description 4
- 229920006362 Teflon® Polymers 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical class [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000003384 imaging method Methods 0.000 abstract description 10
- 238000011065 in-situ storage Methods 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 19
- 230000008901 benefit Effects 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to the field of biotechnology research, a tensile testing device of biological samples, which comprises a shell, a driver, a movable clamp, a movable arm I, an adjusting rod I, a magnet I, a vertical clamp II, a substrate, a movable arm II, an adjusting rod II, a magnet II, a fixed clamp, a liquid storage tank, a supporting platform, a displacement platform, a microscope, a guide rail I and a guide rail II, and has a special driving mechanism and a clamp structure, which can image the living cells in situ at the sub-cell scale and dynamically stretch at the same time, and can stretch the samples uniaxially by combining the special driving mechanism and the clamp structure with adjustable magnetic force, dynamically stretch while imaging the living cells in situ at the sub-cell scale, and study the cell characteristics by in situ imaging, the adjustable range of the stretching frequency and amplitude is larger, and the adjustable range of stretching is larger, and the substrate is not easy to tear.
Description
Technical Field
The utility model belongs to the technical field of biotechnology research and specifically relates to a can be to the living cell normal position imaging of subcellular scale, can carry out the tensile biological sample's of developments simultaneously tensile test device.
Background
In the process of tissue development and repair of cells, mechanical stretching can be used as an important means to adjust the state and function of the cells, and in the prior art, a plurality of methods are used for mechanically stimulating the cells, but most of the prior art adopting a mechanical driving stretching device is not suitable for in-situ imaging of living cells in a sub-cell scale, and in a few of the prior art capable of performing real-time imaging of living cells in the sub-cell scale, dynamic stretching of the cells is limited, and the adjustable frequency range and the adjustable stretching amplitude range of the dynamic stretching device cannot meet the experimental requirements.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem, the utility model discloses the device combines special actuating mechanism and magnetic force adjustable anchor clamps structure to carry out unipolar drawing to the sample, can carry out the developments when the living cell normal position imaging to subcellular dimension and stretch, and the adjustable range of tensile frequency and range is great.
The utility model adopts the technical proposal that:
the tensile testing device for the biological sample comprises a shell, a driver, a movable clamp, a movable arm I, an adjusting rod I, a magnet I, a vertical clamp II, a substrate, a movable arm II, an adjusting rod II, a magnet II, a fixed clamp, a liquid storage tank, a supporting table, a displacement table, a microscope, a guide rail I and a guide rail II, wherein the movable arm I, the adjusting rod I, the vertical clamp II, the substrate, the movable arm II, the adjusting rod II, the magnet II, the fixed clamp, the liquid storage tank, the supporting table, the displacement table, the microscope, the guide rail I and the guide rail II are fixed in the shell, the shell is provided with a plurality of operating windows, protective liquid suitable for a cell culture environment is filled in the liquid storage tank, the microscope is connected with a computer through a cable, the displacement table; the movable clamp comprises a horizontal arm part and a tray part, the horizontal arm part of the movable clamp is connected with the guide rail II in a sliding mode so as to limit the movement of the movable clamp in the z direction, the tray part of the movable clamp is connected to the lower side of the horizontal arm part of the movable clamp, and the tray part of the movable clamp is square in the xz horizontal plane and extends into the liquid storage pool; one end of the movable arm I is hinged to the upper surface of the horizontal arm part of the movable clamp, the other end of the movable arm I is connected with the top end of the vertical clamp I through a screw, the magnet I is fixed on the upper surface of the horizontal arm part of the movable clamp, the adjusting rod I is in threaded connection with the movable arm I and can be adjusted up and down relative to the position of the movable arm I in the y direction, and the lower end of the adjusting rod I can be located in the range of 5 mm to 10 mm above the magnet I; the fixing clamp is fixed on one side of the supporting table and comprises a horizontal arm part and a tray part, the tray part of the fixing clamp is connected to the lower side of the horizontal arm part of the fixing clamp, the tray part of the fixing clamp is square in an xz horizontal plane and extends into the liquid storage pool, one end of the movable arm II is hinged to the upper surface of the horizontal arm part of the fixing clamp, the other end of the movable arm II is connected with the top end of the vertical clamp II through a screw, the magnet II is fixed on the upper surface of the horizontal arm part of the fixing clamp, the adjusting rod II is in threaded connection with the movable arm II and can be adjusted up and down relative to the position of the movable arm II in the y direction, and the lower end of the movable arm II can be; the substrate is a rectangular siloxane film, one end of the substrate is in pressure joint with the upper surface of the tray part of the movable clamp through the lower end of the vertical clamp I, the other end of the substrate is in pressure joint with the upper surface of the tray part of the fixed clamp through the lower end of the vertical clamp II, and the vertical clamp I and the vertical clamp II are both of a sheet structure; the position of the microscope can be adjusted, and the acquired image can be displayed and recorded on a computer; the driver comprises an eccentric cam, a rotary motor, a push rod, a bearing, a connecting block I, a spring, a connecting block II and a sliding block, wherein the eccentric cam, the push rod, the bearing and the connecting block I are of a cam-push rod structure, the eccentric cam and the bearing are respectively in sliding contact with two sides of the push rod, the eccentric cam is a disc with the diameter of 20 mm, the rotating shaft of the eccentric cam deviates from the circle center of the eccentric cam by 5 mm, the eccentric cam is driven to rotate around the rotating shaft of the eccentric cam through the rotary motor, and the rotating frequency of the rotary motor can be adjusted within the range of 0.1 Hz; one end of the connecting block I is connected with a bearing capable of freely rotating, and the other end of the connecting block I is fixedly connected with the movable clamp; one end of the push rod is hinged to the connecting block II, a spring is connected between the push rod and the connecting block II, the elastic coefficient of the spring is adjustable, one end of the connecting block II is hinged to the sliding block, and the sliding block is connected with the guide rail I in a sliding mode so as to limit the moving direction of the sliding block in the x direction; the shell is a metal cavity in a cuboid shape; the movable clamp is made of titanium, the side length of the tray part of the movable clamp is 10 mm, the fixed clamp is made of titanium, and the side length of the tray part of the fixed clamp is 10 mm; the vertical clamp I and the vertical clamp II are both made of Teflon materials; the length of the substrate is 20 mm, and the width of the substrate is 10 mm; the spring has a spring constant in the range of 10 n/m to 50 n/m.
The steps of the experiment carried out by the tensile testing device for the biological sample are as follows:
depositing a cell sample to be detected on the upper surface of a substrate, and respectively pressing two ends of the substrate onto the tray parts of a movable clamp and a fixed clamp through a vertical clamp I and a vertical clamp II;
immersing the tray parts of the movable clamp and the fixed clamp below the liquid level of the protective liquid in the liquid storage tank, and immersing the substrate and the cell sample to be detected below the liquid level;
moving the microscope to a position of 150 mm right above the substrate, imaging the cell sample to be detected, and recording the cell sample in a computer;
turning on a rotary motor, enabling an eccentric cam to act on one side of a push rod, enabling the tension of the substrate to act on the other side of the push rod through a movable clamp, a connecting block I and a bearing, and enabling the movable clamp to reciprocate in the z direction so as to stretch the substrate and a cell sample to be detected on the surface of the substrate;
imaging the cell sample to be detected through a microscope and recording the imaged cell sample in a computer;
step six, changing the rotating frequency of the rotating motor according to the requirement to adjust the stretching frequency of the substrate and the cell sample to be detected on the surface of the substrate;
step seven, adjusting the elastic coefficient of the spring according to the requirement to change the stretching amplitude of the substrate and the cell sample to be detected on the surface of the substrate;
and step eight, analyzing the state and the function of the cell sample to be detected through the imaging information of the cell sample to be detected recorded in the computer.
The utility model has the advantages that:
the utility model discloses the device can carry out unipolar dynamic stretching to the biological cell sample of subcellular scale, and tensile frequency adjustable range is great, and tensile adjustable range is great, and is difficult for causing the substrate to tear.
Drawings
The following is further illustrated in connection with the figures of the present invention:
FIG. 1 is a schematic view of the present invention;
fig. 2 is an enlarged top view of the actuator and the movable clamp.
In the figure, 1, a shell, 2, a driver, 2-1, an eccentric cam, 2-2, a rotary motor, 2-3, a push rod, 2-4, a bearing, 2-5, a connecting block I, 2-6, a spring, 2-7, a connecting block II, 2-8, a sliding block, 3, a movable clamp, 4, a movable arm I, 5, an adjusting rod I, 6, a magnet I, 7, a vertical clamp I, 8, a vertical clamp II, 9, a substrate, 10, a movable arm II, 11, an adjusting rod II, 12, a magnet II, 13, a fixed clamp, 14, a liquid storage tank, 15, a supporting table, 16, a displacement table, 17, a microscope, 18, a guide rail I, 19 and a guide rail II are arranged.
Detailed Description
FIG. 1 is a schematic view of the present invention, which comprises a housing 1 and a driver 2 located in the housing 1, a movable clamp 3, a movable arm I4, an adjusting rod I5, a magnet I6, a vertical clamp I7, a vertical clamp II 8, a substrate 9, a movable arm II 10, an adjusting rod II 11, a magnet II 12, a fixed clamp 13, a liquid storage tank 14, a supporting platform 15, a displacement platform 16, a microscope 17, a guide rail I18 and a guide rail II 19, where xyz is a three-dimensional coordinate system, the housing 1 is a metal cavity in the shape of a cuboid, the housing 1 has a plurality of operating windows, a protection liquid adapted to a cell culture environment is contained in the liquid storage tank 14, the microscope 17 is connected with a computer through a cable, the displacement platform 16, two parallel guide rails I18 and two parallel guide rails II 19 are all fixed in the housing 1, the guide rails I18 and the guide rails II 19, the support platform 15 is positioned above the displacement platform 16, the displacement platform 16 can enable the support platform 15 to move in three dimensions, and the liquid storage tank 14 is fixed above the support platform 15; the movable clamp 3 comprises a horizontal arm part and a tray part, the movable clamp 3 is made of titanium, the horizontal arm part of the movable clamp 3 is connected with a guide rail II 19 in a sliding mode so as to limit the movement of the movable clamp 3 in the z direction, the tray part of the movable clamp 3 is connected to the lower side of the horizontal arm part of the movable clamp 3, the tray part of the movable clamp 3 is square in the xz horizontal plane and extends into the liquid storage tank 14, and the side length of the tray part of the movable clamp 3 is 10 mm; one end of the movable arm I4 is hinged to the upper surface of the horizontal arm part of the movable clamp 3, the other end of the movable arm I4 is connected with the top end of the vertical clamp I7 through a screw, the magnet I6 is fixed on the upper surface of the horizontal arm part of the movable clamp 3, the adjusting rod I5 is in threaded connection with and penetrates through the movable arm I4, the position of the adjusting rod I5 relative to the movable arm I4 in the y direction can be adjusted up and down, and the lower end of the adjusting rod I5 can be located in the range of 5 mm to 10 mm above; the fixing clamp 13 is fixed on one side of the supporting platform 15, the fixing clamp 13 comprises a horizontal arm part and a tray part, the side length of the tray part of the fixing clamp 13 is 10 mm, the fixing clamp 13 is made of titanium, the tray part of the fixing clamp 13 is connected to the lower side of the horizontal arm part of the fixing clamp, the tray part of the fixing clamp 13 is a square in an xz horizontal plane and extends into the liquid storage tank 14, one end of the movable arm II 10 is hinged to the upper surface of the horizontal arm part of the fixing clamp 13, the other end of the movable arm II is connected with the top end of the vertical clamp II 8 through a screw, the magnet II 12 is fixed on the upper surface of the horizontal arm part of the fixing clamp 13, the adjusting, the position of the movable arm II 10 in the y direction can be adjusted up and down, so that the lower end of the movable arm II 10 is positioned in the range of 5 mm to 10 mm above the magnet II 12; the substrate 9 is a rectangular siloxane film, the length of the substrate 9 is 20 mm, the width of the substrate 9 is 10 mm, one end of the substrate 9 is pressed on the tray part of the movable clamp 3 through the lower end of the vertical clamp I7, the other end of the substrate is pressed on the tray part of the fixed clamp 13 through the lower end of the vertical clamp II 8, the vertical clamp I7 and the vertical clamp II 8 are both of sheet structures, and the vertical clamp I7 and the vertical clamp II 8 are both made of Teflon materials; the position of the microscope 17 can be adjusted and the acquired images can be displayed and recorded on a computer.
FIG. 2 is a schematic top enlarged view of a driver and a movable clamp, xyz is a three-dimensional coordinate system, the driver 2 includes an eccentric cam 2-1, a rotary motor 2-2, a push rod 2-3, a bearing 2-4, a connecting block I2-5, a spring 2-6, a connecting block II2-7 and a slider 2-8, the eccentric cam 2-1, the push rod 2-3, the bearing 2-4 and the connecting block I2-5 are in a cam-push rod structure, the eccentric cam 2-1 and the bearing 2-4 are in sliding contact with two sides of the push rod 2-3 respectively, the eccentric cam 2-1 is a disc with a diameter of 20 mm, a rotation axis of the eccentric cam 2-1 is 5 mm away from a center of the circle, the eccentric cam 2-1 is driven to rotate around the rotation axis by the rotary motor 2-2, the rotation frequency of the rotary motor 2-2 can be adjusted in the range of 0.1Hz to 10 Hz; one end of the connecting block I2-5 is connected with a bearing 2-4 which can freely rotate, and the other end is fixedly connected with the movable clamp 3; one end of the push rod 2-3 is hinged to the connecting block II2-7, a spring 2-6 is connected between the push rod and the connecting block II2-7, the elastic coefficient of the spring 2-6 is adjustable, the elastic coefficient range of the spring 2-6 is 10N/m to 50N/m, one end of the connecting block II2-7 is hinged to the sliding block 2-8, and the sliding block 2-8 is in sliding connection with the guide rail I18 so as to limit the moving direction of the sliding block 2-8 in the x direction.
The utility model discloses the principle that the device carries out cell tensile experiment: depositing a cell sample to be detected on the upper surface of a substrate 9, respectively pressing two ends of the substrate 9 on the tray parts of a movable clamp 3 and a fixed clamp 13 through a vertical clamp I7 and a vertical clamp II 8, changing the attraction between the substrate and a magnet I6 by adjusting the position of an adjusting rod I5, and changing the attraction between the substrate and a magnet II 12 by adjusting the position of an adjusting rod II 11, so as to respectively adjust the pressure of the vertical clamp I7 and the vertical clamp II 8 on the substrate 9, and the advantage of the design is that the substrate 9 is prevented from being torn due to overlarge pressure; the tray parts of the movable clamp 3 and the fixed clamp 13 are immersed below the liquid level of the protective liquid in the liquid storage tank 14, the microscope 17 is moved to a position 150 mm above the substrate 9, the cell sample to be measured is imaged through the microscope, the microscope is displayed in a computer, the rotating motor 2-2 is started, the eccentric cam 2-1 rotates around the rotating shaft of the eccentric cam, the push rod 2-3 is caused to move, the connecting block I2-5 and the movable clamp 3 are driven to reciprocate in the z direction through the push rod 2-3, and the substrate 9 is stretched, and the cell sample to be measured deposited on the surface of the substrate 9 is also stretched due to the tension action of the surface of the substrate 9.
Principle of adjusting stretching frequency and stretching amplitude: the rotating speed of the eccentric cam 2-1 is adjusted by changing the rotating frequency of the rotating motor 2-2, so that the frequency of the reciprocating motion of the movable clamp 3 in the z direction, namely the stretching frequency of the substrate 9 and the cell sample to be tested on the surface of the substrate 9 is changed, and the displacement of the tail end of the push rod 2-3 in the rotating process of the eccentric cam 2-1 can be changed by adjusting the elastic coefficient of the spring 2-6, so that the displacement of the connecting block I2-5 and the movable clamp 3 in the reciprocating motion in the z direction is changed, and the stretching amplitude of the substrate 9 and the cell sample to be tested on the surface of the substrate 9 is changed.
The tensile testing device of the biological sample comprises a shell 1, a driver 2, a movable clamp 3, a movable arm I4, an adjusting rod I5, a magnet I6, a vertical clamp I7, a vertical clamp II 8, a substrate 9, a movable arm II 10, an adjusting rod II 11, a magnet II 12, a fixed clamp 13, a liquid storage tank 14, a supporting table 15, a displacement table 16, a microscope 17, a guide rail I18 and a guide rail II 19, wherein xyz is a three-dimensional coordinate system, the shell 1 is provided with a plurality of operating windows, protective liquid suitable for a cell culture environment is filled in the liquid storage tank 14, the microscope 17 is connected with a computer through a cable, the displacement table 16, the two parallel guide rails I18 and the two parallel guide rails II 19 are fixed in the shell 1, the guide rails I18 and the guide rail II 19 are vertical in space, the supporting table 15 is positioned above the displacement table 16, the displacement table 16 can make the support table 15 move three-dimensionally, and the liquid storage tank 14 is fixed on the support table 15; the movable clamp 3 comprises a horizontal arm part and a tray part, the horizontal arm part of the movable clamp 3 is connected with the guide rail II 19 in a sliding way so as to limit the movement of the movable clamp 3 in the z direction, the tray part of the movable clamp 3 is connected to the lower side of the horizontal arm part, and the tray part of the movable clamp 3 is square in the xz horizontal plane and extends into the liquid storage tank 14; one end of the movable arm I4 is hinged to the upper surface of the horizontal arm part of the movable clamp 3, the other end of the movable arm I4 is connected with the top end of the vertical clamp I7 through a screw, the magnet I6 is fixed on the upper surface of the horizontal arm part of the movable clamp 3, the adjusting rod I5 is in threaded connection with and penetrates through the movable arm I4, the position of the adjusting rod I5 relative to the movable arm I4 in the y direction can be adjusted up and down, and the lower end of the adjusting rod I5 can be located in the range of 5 mm to 10 mm above; the fixed clamp 13 is fixed on one side of the support platform 15, the fixed clamp 13 comprises a horizontal arm part and a tray part, the tray part of the fixed clamp 13 is connected to the lower side of the horizontal arm part, the tray part of the fixed clamp 13 is square in an xz horizontal plane and extends into the liquid storage tank 14, one end of the movable arm II 10 is hinged to the upper surface of the horizontal arm part of the fixed clamp 13, the other end of the movable arm II 10 is connected with the top end of the vertical clamp II 8 through a screw, the magnet II 12 is fixed on the upper surface of the horizontal arm part of the fixed clamp 13, the adjusting rod II 11 is in threaded connection with the movable arm II 10 and can be adjusted up and down relative to the position of the movable arm II 10 in the y direction, and the lower end of the movable arm II 10 can be located; the substrate 9 is a rectangular siloxane film, one end of the substrate 9 is pressed on the tray part of the movable clamp 3 through the lower end of the vertical clamp I7, the other end of the substrate 9 is pressed on the tray part of the fixed clamp 13 through the lower end of the vertical clamp II 8, and the vertical clamp I7 and the vertical clamp II 8 are both of sheet structures; the position of the microscope 17 can be adjusted and the acquired images can be displayed and recorded on a computer; the driver 2 comprises an eccentric cam 2-1, a rotary motor 2-2, a push rod 2-3, a bearing 2-4, a connecting block I2-5, a spring 2-6, a connecting block II2-7 and a slide block 2-8, wherein the eccentric cam 2-1, the push rod 2-3, the bearing 2-4 and the connecting block I2-5 are of a cam-push rod structure, the eccentric cam 2-1 and the bearing 2-4 are respectively in sliding contact with two sides of the push rod 2-3, the eccentric cam 2-1 is a disc with the diameter of 20 mm, the rotating shaft of the eccentric cam 2-1 deviates from the circle center of the eccentric cam by 5 mm, the eccentric cam 2-1 is driven to rotate around the rotating shaft by the rotating motor 2-2, and the rotating frequency of the rotating motor 2-2 can be adjusted within the range of 0.1Hz to 10 Hz; one end of the connecting block I2-5 is connected with a bearing 2-4 which can freely rotate, and the other end is fixedly connected with the movable clamp 3; one end of the push rod 2-3 is hinged to the connecting block II2-7, a spring 2-6 is connected between the push rod and the connecting block II2-7, the elastic coefficient of the spring 2-6 is adjustable, one end of the connecting block II2-7 is hinged to the sliding block 2-8, and the sliding block 2-8 is in sliding connection with the guide rail I18 so as to limit the moving direction of the sliding block 2-8 in the x direction; the shell 1 is a metal cavity in a cuboid shape; the movable clamp 3 is made of titanium, the side length of the tray part of the movable clamp 3 is 10 mm, the fixed clamp 13 is made of titanium, and the side length of the tray part of the fixed clamp 13 is 10 mm; the vertical clamp I7 and the vertical clamp II 8 are both made of Teflon materials; the length of the substrate 9 is 20 mm and the width is 10 mm; the spring 2-6 has a spring constant in the range of 10 n/m to 50 n/m.
The utility model discloses have special actuating mechanism and anchor clamps structure, can carry out unipolar dynamic stretching to the biological cell sample of subcellular scale to study the cell characteristic through the normal position imaging, the adjustable range of tensile frequency and tensile range is great.
Claims (6)
1. A tensile test device of a biological sample comprises a shell (1), a driver (2) positioned in the shell (1), a movable clamp (3), a movable arm I (4), an adjusting rod I (5), a magnet I (6), a vertical clamp I (7), a vertical clamp II (8), a substrate (9), a movable arm II (10), an adjusting rod II (11), a magnet II (12), a fixed clamp (13), a liquid storage tank (14), a supporting table (15), a displacement table (16), a microscope (17), a guide rail I (18) and a guide rail II (19), wherein xyz is a three-dimensional coordinate system, the shell (1) is provided with a plurality of operating windows, protective liquid suitable for a cell culture environment is filled in the liquid storage tank (14), and the microscope (17) is connected with a computer through a cable,
the method is characterized in that: the displacement table (16), the two parallel guide rails I (18) and the two parallel guide rails II (19) are all fixed in the shell (1), the guide rails I (18) and the guide rails II (19) are vertical in space, the support table (15) is positioned above the displacement table (16), the displacement table (16) can enable the support table (15) to move in a three-dimensional mode, and the liquid storage tank (14) is fixed above the support table (15); the movable clamp (3) comprises a horizontal arm part and a tray part, the horizontal arm part of the movable clamp (3) is connected with the guide rail II (19) in a sliding mode so as to limit the movement of the movable clamp (3) in the z direction, the tray part of the movable clamp (3) is connected to the lateral lower portion of the horizontal arm part of the movable clamp, and the tray part of the movable clamp (3) is square in the xz horizontal plane and extends into the liquid storage tank (14); one end of a movable arm I (4) is hinged to the upper surface of the horizontal arm part of the movable clamp (3), the other end of the movable arm I (4) is connected with the top end of a vertical clamp I (7) through a screw, a magnet I (6) is fixed to the upper surface of the horizontal arm part of the movable clamp (3), an adjusting rod I (5) is in threaded connection with and penetrates through the movable arm I (4), the position of the adjusting rod I (5) relative to the movable arm I (4) in the y direction can be adjusted up and down, and the lower end of the adjusting rod I (5) can be located in the range of 5 mm to 10 mm above; the fixing clamp (13) is fixed on one side of the supporting table (15), the fixing clamp (13) comprises a horizontal arm part and a tray part, the tray part of the fixing clamp (13) is connected to the lower side of the horizontal arm part, the tray part of the fixing clamp (13) is square in an xz horizontal plane and extends into the liquid storage tank (14), one end of the movable arm II (10) is hinged to the upper surface of the horizontal arm part of the fixing clamp (13), the other end of the movable arm II (10) is connected with the top end of the vertical clamp II (8) through a screw, the magnet II (12) is fixed on the upper surface of the horizontal arm part of the fixing clamp (13), and the adjusting rod II (11) is in threaded connection with and penetrates through the, the position of the movable arm II (10) in the y direction can be adjusted up and down, so that the lower end of the movable arm II (10) is positioned in the range of 5 mm to 10 mm above the magnet II (12); the substrate (9) is a rectangular siloxane film, one end of the substrate (9) is pressed on the tray part of the movable clamp (3) through the lower end of the vertical clamp I (7), the other end of the substrate is pressed on the tray part of the fixed clamp (13) through the lower end of the vertical clamp II (8), and the vertical clamp I (7) and the vertical clamp II (8) are both of a sheet structure; the position of the microscope (17) can be adjusted, and the acquired images can be displayed and recorded on a computer;
the driver (2) comprises an eccentric cam (2-1), a rotating motor (2-2), a push rod (2-3), a bearing (2-4), a connecting block I (2-5), a spring (2-6), a connecting block II (2-7) and a sliding block (2-8), wherein the eccentric cam (2-1), the push rod (2-3), the bearing (2-4) and the connecting block I (2-5) are of a cam-push rod structure, the eccentric cam (2-1) and the bearing (2-4) are respectively in sliding contact with two sides of the push rod (2-3), the eccentric cam (2-1) is a disc with the diameter of 20 mm, the rotating shaft of the eccentric cam (2-1) deviates from the circle center of the eccentric cam by 5 mm, and the rotating motor (2-2) drives the eccentric cam (2-1) to rotate around the rotating shaft, the rotation frequency of the rotary motor (2-2) can be adjusted in the range of 0.1Hz to 10 Hz; one end of the connecting block I (2-5) is connected with a bearing (2-4) capable of freely rotating, and the other end is fixedly connected with the movable clamp (3); one end of the push rod (2-3) is hinged to the connecting block II (2-7) and connected with the spring (2-6) between the push rod and the connecting block II (2-7), the elastic coefficient of the spring (2-6) is adjustable, one end of the connecting block II (2-7) is hinged to the sliding block (2-8), and the sliding block (2-8) is in sliding connection with the guide rail I (18) so as to limit the moving direction of the sliding block (2-8) in the x direction.
2. The device for tensile testing of a biological specimen according to claim 1, wherein: the shell (1) is a metal cavity in a cuboid shape.
3. The device for tensile testing of a biological specimen according to claim 1, wherein: the movable clamp (3) is made of titanium, the side length of the tray part of the movable clamp (3) is 10 mm, the fixed clamp (13) is made of titanium, and the side length of the tray part of the fixed clamp (13) is 10 mm.
4. The device for tensile testing of a biological specimen according to claim 1, wherein: the vertical clamp I (7) and the vertical clamp II (8) are both made of Teflon materials.
5. The device for tensile testing of a biological specimen according to claim 1, wherein: the substrate (9) has a length of 20 mm and a width of 10 mm.
6. The device for tensile testing of a biological specimen according to claim 1, wherein: the spring coefficient of the spring (2-6) ranges from 10N/m to 50N/m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921133400.4U CN210533960U (en) | 2019-07-10 | 2019-07-10 | Tensile testing device for biological sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921133400.4U CN210533960U (en) | 2019-07-10 | 2019-07-10 | Tensile testing device for biological sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210533960U true CN210533960U (en) | 2020-05-15 |
Family
ID=70598323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921133400.4U Withdrawn - After Issue CN210533960U (en) | 2019-07-10 | 2019-07-10 | Tensile testing device for biological sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210533960U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110376123A (en) * | 2019-07-10 | 2019-10-25 | 金华职业技术学院 | A kind of tensile test device of biological sample |
-
2019
- 2019-07-10 CN CN201921133400.4U patent/CN210533960U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110376123A (en) * | 2019-07-10 | 2019-10-25 | 金华职业技术学院 | A kind of tensile test device of biological sample |
| CN110376123B (en) * | 2019-07-10 | 2024-06-18 | 金华职业技术学院 | Tensile testing device for biological sample |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1267719C (en) | Reciprocal and rotary type incorporated frictional wear test machine | |
| CN101500767B (en) | Method and device for cutting fresh tissue slices | |
| CN201212871Y (en) | Test system for dynamic bending fatigue performance of thin-film material | |
| CN102519867B (en) | Direct-acting soft friction testing apparatus | |
| CN210533960U (en) | Tensile testing device for biological sample | |
| CN106248482B (en) | Triaxial test device and method suitable for weak rock | |
| CN108444816B (en) | Rock mass structural plane cyclic shear tester and test method | |
| CN206300855U (en) | Flexural fatigue tester and test system | |
| CN2702298Y (en) | Reciprocal and rotary type integral frictional wear experiment machine | |
| CN110376123B (en) | Tensile testing device for biological sample | |
| CN113654879B (en) | Clamping device capable of testing torsional force and shearing force of textile material and testing method | |
| CN101825542A (en) | Small load surface scratch testing device | |
| CN108489838A (en) | Contact surface in-situ observation frictional wear experiment platform | |
| CN105675426B (en) | The double sample in situ imaging devices of vacuum pin disc type abrasion tester one camera | |
| CN110376124B (en) | Biological sample tensile test method | |
| CN109682462B (en) | Test device for researching friction vibration noise of elastomer material | |
| CN209894615U (en) | Visual flexible material bending performance measuring device | |
| CN110220785B (en) | Gel stretching device | |
| CN108414355A (en) | A kind of film tensile loads unit with position lock function | |
| CN211061620U (en) | Experimental device for high-speed train pantograph self-adaptation active control | |
| CN109799134A (en) | A kind of superhigh precision friction wear testing machine carrying out rubbing surface in-situ observation | |
| CN112606169B (en) | Device and method for 3D printing of experimental part contacted with undisturbed soil | |
| CN214612499U (en) | Special translation mechanism of stem cell detection | |
| CN212077073U (en) | Power-electricity coupling loading platform | |
| CN114199455A (en) | Automatic measurement and control system for mechanical metrological verification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20200515 Effective date of abandoning: 20240618 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20200515 Effective date of abandoning: 20240618 |
|
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |