CN210481398U

CN210481398U - Marine lysozyme fermentation process control device

Info

Publication number: CN210481398U
Application number: CN201920778275.6U
Authority: CN
Inventors: 陈永琪; 诸紫燕
Original assignee: JIANGSU KEHAI BIO-ENGINEERING EQUIPMENT CO LTD
Current assignee: JIANGSU KEHAI BIO-ENGINEERING EQUIPMENT CO LTD
Priority date: 2019-05-28
Filing date: 2019-05-28
Publication date: 2020-05-08
Anticipated expiration: 2029-05-28

Abstract

The utility model relates to a marine lysozyme fermentation process controlling means, the device include fermentation cylinder (1), parameter measurement device, singlechip (32), PLC control module (33), opto-coupler drive circuit (35), actuating mechanism and host computer (36). The single chip microcomputer is embedded with a marine lysozyme fermentation key parameter soft measurement algorithm, can predict substrate concentration, thallus concentration and relative enzyme activity in the fermentation process, performs decoupling through the algorithm, calculates the variable quantity of the environmental parameter to be adjusted, and sends the variable quantity into the PLC control module. The PLC controller adopts fuzzy PID to control the temperature and the pH value, and controls the stirring rotating speed, the pressure in the tank and the feeding rate through conventional PID. The multi-point acquisition of the environmental parameters in the fermentation process and the decoupling control of the key parameters are realized, the reliability of the system is improved, and the control is more accurate and reliable.

Description

Marine lysozyme fermentation process control device

Technical Field

The utility model relates to a be difficult to real-time measurement and control for solving among the marine lysozyme fermentation process key parameter (matrix concentration, thallus concentration and relative enzyme activity), belong to marine lysozyme apparatus for producing's technical field.

Background

The marine lysozyme has the characteristics of oxidation resistance and wide bacteriolytic spectrum, can specifically act on bacterial cell wall peptidoglycan molecules to enable the bacterial cell wall to become loose so as to achieve the effect of bacteriolytic, and the product is applied to a plurality of fields such as medical treatment, food industry, livestock and poultry breeding, bioengineering and the like. With the rapid development and widespread use of antibiotic production, two significant problems arise clinically: firstly, the drug resistance of bacteria is increased year by year, so that the curative effect of some antibiotics is reduced or even ineffective; secondly, some nonpathogenic bacteria become conditional pathogenic bacteria. Therefore, the demand of lysozyme is more and more, and the existing quantity of lysozyme is difficult to solve the contradiction between supply and demand of the market. The soft measurement and control of key parameters in the fermentation process of the marine lysozyme can control the progress degree of the fermentation process, are beneficial to various substrate materials supplementation and the control of the metabolism of microorganisms, control the quality of fermentation products, and have important practical significance for the industrial production of the marine lysozyme.

In the traditional control mode, a single singlechip or a single PLC control method is often adopted. The single chip microcomputer has the advantages of high integration level, small volume, strong control function, easiness in expansion, low power consumption and the like, is high in cost performance and is commonly used in a data acquisition system; however, the main control board manufactured by the single chip microcomputer is influenced by factors such as a plate manufacturing process, a layout structure and device quality, so that the anti-interference capability is poor, the failure rate is high, the dependence on the environment is strong, and the development period is long. The PLC is a controller which is tested in practical application for decades, and has the advantages of strong anti-interference capability, low failure rate, easy equipment expansion, convenient maintenance, high reliability and stability and short development period; the architecture of the PLC is closed, requires specific control procedures, and learns specific programming languages.

Disclosure of Invention

The technical problem is as follows: the utility model aims at providing a marine lysozyme fermentation process controlling means adopts PLC and singlechip coordinated control, and the singlechip is sent to through the data line after the data process of sensor will gathering, imbeds intelligent algorithm in the singlechip, can realize the multiple spot collection to fermentation process environmental parameter, the decoupling control of key parameter, can carry out acquisition control to all kinds of parameters by the great range. The PLC is more and more widely applied, other control systems with high energy consumption, large volume and poor reliability are gradually replaced at present, and the PLC and the single chip microcomputer are in linkage control, so that the instability of single chip microcomputer control and poor anti-interference capability are avoided, the reliability of the system is improved, and the control is more accurate and reliable.

The technical scheme is as follows: the utility model discloses a marine lysozyme fermentation process control device, including fermentation cylinder, parameter measurement device, analog signal regulator, multi-way selector switch CD4051, variable gain amplifier AD526, singlechip, PLC control module, 8255 parallel port expansion mouth, opto-coupler drive circuit, actuating mechanism and host computer;

various sensors among the parameter measurement device pass through analog signal adjuster, multiple selector switch CD4051 and variable gain amplifier AD526 and connect in AD conversion module, AD conversion module connects in the singlechip, the singlechip via GPIO module connection with PLC control module, PLC control module connects 8255 parallel port expansion mouth, is used for controlling actuating mechanism work with signal transmission to opto-coupler drive circuit, actuating mechanism is used for adjusting and showing temperature, pressure, stirring rotational speed, dissolved oxygen concentration, pH parameter when fermenting in the host computer.

Wherein:

the fermentation tank is provided with a temperature sensor T1(Pt100), a first flow meter, a DO sensor, a pH sensor, a pressure meter, a photoelectric encoder, a temperature sensor T2(Pt100) and a second flow meter which are respectively used for detecting parameters such as temperature, air flow, dissolved oxygen concentration, pH value, pressure, motor rotating speed and the like in the marine lysozyme fermentation tank.

The outer layer of the marine lysozyme fermentation tank is provided with a jacket, the top of the jacket is provided with a water inlet, the bottom of the jacket is provided with a water outlet, the water inlet and the water outlet are connected in a water tank, the water tank is connected with a cooling water outlet, and a heater is arranged in the water tank; actuating mechanism includes the speed regulator that is connected with rabbling mechanism, the first air inlet electrical control valve that is connected with fermentation cylinder upper portion input pipeline, second air inlet electrical control valve and third air inlet electrical control valve, the cooling water solenoid valve and the heater that are connected with the water supply system basin, the peristaltic pump for the first benefit acid that is connected with fermentation agitating unit, peristaltic pump for the second benefit alkali, peristaltic pump 3 for the benefit defoaming liquid, peristaltic pump for the first benefit and the peristaltic pump for the second benefit.

The PLC is controlled by the linkage of a PLC and a single chip microcomputer, the substrate concentration, the thallus concentration and the relative enzyme activity in the fermentation process are predicted, the variable quantity of the environmental parameters required to be adjusted is calculated, and the variable quantity is sent to a PLC control module to be adjusted by the module; sending a signal by a PLC control module: opening a cooling water electromagnetic valve to control the inflow of cooling water or controlling a heater to work to control the temperature of water flowing into a jacket; the second control peristaltic pump is used for alkali supplement, and the first control peristaltic pump is used for acid supplement and pH adjustment; the switch of the first air inlet electric regulating valve, the second air inlet electric regulating valve and the third air inlet electric regulating valve regulates the concentration of dissolved oxygen in the tank, the pressure in the tank and the air flow by controlling the rotating speed of the stirring motor; thirdly, defoaming liquid is added by controlling a peristaltic pump; and the fourth peristaltic pump and the fifth peristaltic pump are controlled to supplement materials, and the rotating speed of the motor is controlled by the speed regulator, so that the supplemented materials are more uniformly added into the fermentation liquor.

Has the advantages that: the utility model discloses regulation to the temperature is accomplished through cooling water solenoid valve and heater, and when jar internal temperature was lower, the switch and the heating power of adjustment heater rose the temperature that flows in the jacket, drove the rising of jar internal temperature, when needing to reduce jar internal temperature, opened the cooling water solenoid valve, and communication recirculated cooling water makes the temperature reduce, because temperature variation has stronger time hysteresis in the fermentation cylinder, consequently adopts fuzzy theory to set the PID parameter. Also the control of pH is time-dependent and non-linear, so the actuators controlling pH: the peristaltic pump for first acid supplement and the peristaltic pump for second alkali supplement are controlled by fuzzy PID.

Drawings

The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.

FIG. 1 is a schematic structural view of the device for controlling the fermentation process of marine lysozyme in accordance with the present invention;

fig. 2 is a schematic diagram of the connection relationship between the modules of the control device according to the present invention.

A fermentation tank 1, a jacket 2, a water inlet 3, a water outlet 4, a water tank 5, a cooling water outlet 6, a first temperature sensor 7, a first flow meter 8, a DO sensor 9, a pH sensor 11, a pressure meter 13, a stirring motor 14, a photoelectric encoder 15, a second temperature sensor 16, a second flow meter 17, a speed regulator 18 and a first air inlet electric regulating valve 19, the system comprises a second air inlet electric regulating valve 20, a first air inlet electric regulating valve 21, a cooling water electromagnetic valve 22, a heater 23, a first acid supplementing peristaltic pump 24, a second alkali supplementing peristaltic pump 25, a third foam supplementing and removing liquid peristaltic pump 26, a first material supplementing peristaltic pump 27, a second material supplementing peristaltic pump 28, an analog signal regulator 29, a multi-way selector switch CD 405130, a variable gain amplifier AD 52631, a single chip microcomputer 32, a PLC control module 33, an 8255 parallel port expansion port 34, an optical coupler driving circuit 35, an execution mechanism and an upper computer 36.

Detailed Description

The invention is further described with reference to the following figures and specific examples.

Fig. 1 is the structural schematic diagram of the marine lysozyme fermentation process control device of the utility model, which comprises a fermentation tank 1, a parameter measuring device, an analog signal adjuster 29, a multi-way selector switch CD4051, a variable gain amplifier AD526, a single chip microcomputer 32, a PLC controller module 33, an 8255 parallel port expansion port 34, an optical coupler driving circuit 35, an actuating mechanism and an upper computer 36.

Various sensors in the parameter measuring device are connected to an A/D conversion module through an analog signal adjuster 29, a multi-way selector switch CD4051 and a variable gain amplifier AD526, the A/D conversion module is connected to a single chip microcomputer 32, the single chip microcomputer 32 is connected with a PLC control module 33 through a GPIO module, the PLC control module 33 is connected with an 8255 parallel port expansion port 34, signals are sent to an optical coupling driving circuit 35 to control an executing mechanism to work, and the executing mechanism is used for adjusting parameters such as temperature, pressure, stirring rotating speed, dissolved oxygen concentration and pH during fermentation;

the sensor is used for detecting the analog signals of parameters such as temperature, pressure, stirring speed, dissolved oxygen concentration and pH value during the fermentation of the marine lysozyme through the analog signal regulator 29, the analog signals are converted into digital signals through the A/D conversion module and then sent into the single chip microcomputer 32 for soft measurement of three state quantities such as substrate concentration, thallus concentration and relative enzyme activity, corresponding environmental parameter variation is calculated through a decoupling algorithm and sent to the PLC control module 33, the operation of the executing mechanism is controlled, so that the parameters such as temperature, pressure, oxygen concentration and pH value during the fermentation are regulated, and meanwhile, the relevant parameters during the fermentation are displayed on the upper computer 36.

Further, the sensors comprise a first temperature sensor T1(Pt100), a first flow meter 8, a DO sensor 9, a pH sensor 11, a pressure meter 13, a photoelectric encoder 15, a second temperature sensor T2(Pt100)16 and a second flow meter 17 which are respectively used for detecting parameters such as temperature, air flow, dissolved oxygen concentration, pH value, pressure, motor rotating speed and the like in the marine lysozyme fermentation tank 1.

The actuating mechanism comprises a speed regulator 18, a first air inlet electric regulating valve 19, a second air inlet electric regulating valve 20, a first air inlet electric regulating valve 21, a cooling water electromagnetic valve 22, a heater 23, a first acid supplementing peristaltic pump 24, a second alkali supplementing peristaltic pump 25, a third defoaming liquid supplementing peristaltic pump 26, a first material supplementing peristaltic pump 27 and a second material supplementing peristaltic pump 28; the outer layer of the marine lysozyme fermentation tank 1 is provided with a jacket 2, the top of the jacket 2 is provided with a water inlet 3, the bottom of the jacket is provided with a water outlet 4, the water inlet 3 and the water outlet 4 are connected in a water tank 5, the water tank 5 is connected with a cooling water outlet 6, and a heater 23 is arranged in the water tank 5.

Furthermore, the model selected by the first temperature sensor T1 and the second temperature sensor T2 is CWDZ11, a two-wire system direct current output of 4-20 mA is adopted, temperature drift and nonlinear automatic compensation can be carried out, the temperature range is-50-100 ℃, the minimum range is 0-100 ℃, and the precision is 0.5% FS.

When the output of the singlechip 32 is judged and the temperature needs to be raised, the heater 23 is controlled to work, and the heating power of the heater 23 is set; when the temperature in the tank needs to be reduced, the PLC control module 33 adopts a fuzzy PID algorithm to send out a signal, and the cooling water electromagnetic valve 22 is opened to control the inflow of cooling water, so as to reduce the temperature of the water flowing into the jacket 2.

Furthermore, the product model selected by the pH sensor 11 is XW-400, a two-wire system 4-20 mA direct current output is adopted, a strong acid and alkali resistant high-temperature resistant glass electrode is adopted, a low-noise cable is adopted, an output signal is up to 30m and is free of interference, the pH value measurement range is 0-14 pH, the measurement precision is less than 0.01pH, the applicable temperature is 0-90 ℃, the response time is 5sec, and the withstand voltage is 0.6 MP.

When the calculation result of the algorithm in the single chip microcomputer 32 judges that the pH value needs to be increased, the PLC control module 33 adopts a fuzzy PID algorithm to send out a signal, and the second control peristaltic pump 25 performs alkali supplement; when the calculation structure judges that the pH value is too high, the PLC control module 33 controls the peristaltic pump 24 to supplement acid;

furthermore, the model of the dissolved oxygen concentration sensor 9 is SIN-DM2800, the isolated transmission is performed to output 4-20 mA direct current, the measurement range of the dissolved oxygen is 0-20 mg/L, the resolution is 0.01mg/L, the saturation range is 0-200%, the resolution is 0.10%, the oxygen partial pressure range is 0-400 hPa, the resolution is 1hPa, the comprehensive precision is less than 1.5%, the temperature compensation range is-10-60 ℃, and the resolution is 0.1 ℃.

Furthermore, the model of the product selected by the pressure sensor 13 is SIN-P310, a two-wire system direct current output of 4-20 mA is adopted, the pressure measuring range is-0.1-60 MPa, the using temperature is-40-80 ℃, the medium temperature is-40-80 ℃, and the pressure measuring device is used in the fermentation process of the marine lysozyme.

Further, the product model selected by the first flow meter 8 and the second flow meter 17 is LZB-3, the flow range of liquid is 4-40 mL/min, the flow range of gas is 60-600 mL/min, the precision grade is 4 grade, the pressure resistance is less than 0.25MPa, and the allowable temperature range is-20-70 ℃.

When the single chip microcomputer 32 judges that the concentration of dissolved oxygen, the pressure in the tank and the air flow need to be adjusted in the next action, the PLC control module 33 controls the rotating speed of the stirring motor 14 and the opening and closing of the first air inlet electric regulating valve 19, the second air inlet electric regulating valve 20 and the third air inlet electric regulating valve 21 by adopting a conventional PID algorithm.

Furthermore, the photoelectric encoder 15 is an incremental photoelectric encoder with the model of CHB38S, the pulse number of the incremental photoelectric encoder is 10-5000 cpr, the power supply voltage is 5-24V, the response frequency is 80KHz, the consumed current is 60mA, the mechanical rotation speed is 6000rpm, the working temperature is-20-85 ℃, and a cable is provided with a shielding net, so that the dry interference capability is improved to a limited extent.

When the single chip microcomputer 32 judges that the next action needs to adjust the rotating speed of the motor or needs to perform material supplement and defoaming, the PLC control module 33 adopts a conventional PID control algorithm to send out a signal. When defoaming is required, the third control peristaltic pump 26 adds defoaming liquid; when the calculation structure judges that the rotating speed of the motor is too high or too low, the PLC control module 33 controls the speed regulator 18 to regulate the speed; when the calculation structure judges that the material supplement in the fermentation liquor is lack, the PLC control module 33 controls the fourth peristaltic pump 27 and the fifth peristaltic pump 28 to add the material supplement, and simultaneously controls the rotating speed of the stirring motor 14 through the speed regulator 18, so that the material supplement is more uniformly added into the fermentation liquor.

Fig. 2 is a schematic diagram of the connection relationship between modules of the control device of the present invention, the bottom data acquisition part includes the fermentation tank 1, a/D converters of each channel, a plurality of industrial sensors, etc., which together complete the field data acquisition function, send the acquired data to the single chip microcomputer 32, and predict through the algorithm embedded in the single chip microcomputer 32; the calculated environmental parameter adjustment quantity is input into the PLC control module 33 through serial port communication, and if the predicted value is greatly different from the actual value, the field switching quantity can be controlled through the PLC control module 33, so that the accurate prediction of the key parameter is realized; the control process can be displayed on the PC 36 or remotely controlled through serial communication.

Claims

1. A marine lysozyme fermentation process controlling means which characterized in that: the device comprises a fermentation tank (1), a parameter measuring device, an analog signal regulator (29), a multi-way selection switch CD4051 (30), a variable gain amplifier AD526 (31), a single chip microcomputer (32), a PLC control module (33), an 8255 parallel port expansion port (34), an optical coupling drive circuit (35), an execution mechanism and an upper computer (36);

various sensors among the parameter measurement device pass through analog signal adjuster (29), multiple-way selector switch CD4051 (30) and variable gain amplifier AD526 (31) and connect in AD conversion module, AD conversion module connects in singlechip (32), singlechip (32) connect with PLC control module (33) via GPIO module, PLC control module (33) are connected 8255 and are mouthful expanded mouth (34), be used for controlling actuating mechanism work with signal transmission to opto-coupler drive circuit (35), actuating mechanism is arranged in adjusting and showing in host computer (36) temperature, pressure, stirring rotational speed, dissolved oxygen concentration, pH parameter when fermenting.

2. The marine lysozyme fermentation process control device of claim 1, wherein: the fermentation tank (1) is provided with a first temperature sensor (7), a Pt100, a first flow meter (8), a DO sensor (9), a pH sensor (11), a pressure meter (13), a photoelectric encoder (15), a second temperature sensor (16) and a second flow meter (17) which are respectively used for detecting parameters such as temperature, air flow, dissolved oxygen concentration, pH value, pressure, motor rotating speed and the like in the fermentation tank (1) for the ocean lysozyme;

the outer layer of the marine lysozyme fermentation tank (1) is provided with a jacket (2), the top of the jacket (2) is provided with a water inlet (3), the bottom of the jacket is provided with a water outlet (4), the water inlet (3) and the water outlet (4) are connected in a water tank (5), the water tank (5) is connected with a cooling water outlet (6), and a heater (23) is arranged in the water tank (5); the actuating mechanism comprises a speed regulator (18) connected with the stirring mechanism, a first air inlet electric regulating valve (19), a second air inlet electric regulating valve (20) and a third air inlet electric regulating valve (21) which are connected with an upper input end pipeline of the fermentation tank (1), a cooling water electromagnetic valve (22) and a heater (23) which are connected with a water supply system water tank (5), a first acid supplementing peristaltic pump (24), a second alkali supplementing peristaltic pump (25), a defoaming liquid supplementing peristaltic pump 3 (26), a first material supplementing peristaltic pump (27) and a second material supplementing peristaltic pump (28) which are connected with the fermentation stirring device.