CN210419963U - Deep sea microbe in-situ planting enrichment culture tank capable of being automatically sealed - Google Patents

Deep sea microbe in-situ planting enrichment culture tank capable of being automatically sealed Download PDF

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Publication number
CN210419963U
CN210419963U CN201921256935.0U CN201921256935U CN210419963U CN 210419963 U CN210419963 U CN 210419963U CN 201921256935 U CN201921256935 U CN 201921256935U CN 210419963 U CN210419963 U CN 210419963U
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China
Prior art keywords
culture tank
connecting rod
cover
guide block
cover connecting
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Expired - Fee Related
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CN201921256935.0U
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Chinese (zh)
Inventor
周小安
刘军
徐建华
郭大伟
张桥
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China University of Geosciences Beijing
Hangzhou Dianzi University
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China University of Geosciences Beijing
Hangzhou Dianzi University
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Abstract

The utility model relates to the field of deep sea scientific research or microbiology devices, in particular to a deep sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed, which comprises a tank body and a sealing cover, wherein the outer wall of the tank body is fixed with a guide block, and the guide block is provided with a guide hole; the sealing cover is connected with a cover connecting rod, one end of the cover connecting rod is hinged with the sealing cover, and the other end of the cover connecting rod is hinged with the guide block; a movable guide rod is arranged in the guide hole in a matching way, and the upper end of the movable guide rod is arranged in contact with the cover connecting rod; the outer wall of the tank body is also provided with a return spring, and the other end of the return spring is connected with the cover connecting rod; the utility model discloses a related culture tank has solved seabed enrichment culture tank and has ended in the cultivation, and culture tank recovery in-process, inside the sea water gushed culture tank, destroys the problem of the enrichment sample of microorganism in the culture tank, and this utility model relates to a culture tank simple structure is with low costs, does not need electromechanical control device, and the using-way is simple, has improved enrichment sampling device's reliability.

Description

Deep sea microbe in-situ planting enrichment culture tank capable of being automatically sealed
Technical Field
The utility model relates to a marine science or microbiology device field particularly, relates to a deep sea microorganism normal position definite value enrichment culture tank that can seal automatically.
Background
Scientific research shows that the special characteristics of deep sea environment can be utilized to culture and culture microbes with special functions in a planting way. Enrichment culture of deep-sea microorganisms is a long-time process, and generally adopts a microorganism culture tank, nutrients are placed in the tank in advance and carried on a deep-sea long-term workstation to work on the seabed for a long time, and the enrichment culture tank is ensured to be in the deep-sea environment for ensuring that the interior of the enrichment culture tank is communicated with the external seawater environment during the operation on the seabed; therefore, the culture tank is required to be in an open state, the tank opening of the culture tank is open, but after the enrichment culture is finished, a large amount of seawater flows into the culture tank along with the process that the culture tank rises from the seabed along with the seabed workstation, so that the effect of field planting of culture samples is influenced by the effect of interference or damage on microorganisms in the culture tank.
The seal of the deep sea in-situ planting enrichment culture tank is generally realized by controlling the action of a seal cover through a control device. As the in-situ enrichment of microorganisms generally can only take months or more than one year when the seabed works, a better directional enrichment effect of microorganisms can be generated. The use of a control means requires a long-term effective battery of high reliability and a highly reliable control means, and also requires knowledge of when to retrieve the subsea workstation in order to control the sealing means to initiate the sealing action before retrieval. The mode adopting the control technology not only needs reliable deep sea remote control communication technology, deep sea power supply device, deep sea sealing mechanism drive and the like, but also needs to preset sealing time or send sealing signals remotely, which has great technical difficulty and high cost in offshore, especially deep sea application.
Therefore, the design of a culture tank with simple structure, simple operation and low use cost and good sealing effect in the recovery process is an important content in the current deep-sea microorganism planting culture sampling.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a main aim at provides a deep sea normal position microorganism enrichment culture tank that can seal automatically to solve among the prior art seabed enrichment culture tank and finishing, culture tank recovery in-process, the sea water is inside from the culture tank opening part gushing into culture tank, pollutes the problem of the interior microorganism sample of culture tank.
In order to achieve the above object, the utility model adopts the following technical scheme:
an automatically-sealable deep-sea microorganism in-situ planting enrichment culture tank comprises a tank body and a sealing cover, wherein a guide block is fixed on the outer wall of the tank body, and a guide hole is formed in the guide block; the sealing cover is connected with a cover connecting rod, one end of the cover connecting rod is hinged with the sealing cover, and the other end of the cover connecting rod is hinged with the guide block; a movable guide rod is arranged in the guide hole in a matching way, and the upper end of the movable guide rod is arranged in contact with the cover connecting rod; the outer wall of the tank body is also provided with a return spring, and the other end of the return spring is connected with the cover connecting rod.
Furthermore, the guide block comprises an upper guide block and a lower guide block, guide holes are formed in the upper guide block and the lower guide block, and the guide holes are coaxial.
Further, the top of the tank body is provided with a tank end cover, a sealing soft cushion is arranged on the tank end cover, and the tank end cover is provided with a middle opening.
Furthermore, the top of the sealing cover is fixed with a cover connecting block, and the cover connecting block and the cover connecting rod are hinged together through a pin shaft I.
Furthermore, the cover connecting rod is L-shaped, a supporting block is fixed on the upper guide block, and the supporting block is hinged with one end of the cover connecting rod through a pin shaft II.
Furthermore, a connecting shaft is fixed on the cover connecting rod, and two ends of the connecting shaft are respectively connected with a return spring.
Furthermore, a section of thread is arranged on the movable guide rod between the upper guide block and the lower guide block, and the positioning nut is connected to the movable guide rod.
Furthermore, the bottom of the tank body is provided with a fixing plate for fixing the culture tank on the support bottom plate.
The utility model discloses following beneficial effect has:
the deep sea microbe in-situ planting enrichment culture tank capable of being automatically sealed is provided with a mechanical linkage automatic sealing device, and when a seabed microbe sample is planted, enriched and cultured in the culture tank, a movable guide rod is jacked up by a disposable ballast block; in income water and seabed enrichment in-process, under the support of activity guide arm, the lid connecting rod drives sealed lid and opens, the jar mouth of culture tank is opened, abandon the ballast piece when the culture tank needs to be retrieved to the end of the enrichment process, the culture tank rises along with the workstation, at this moment, the supporting role of activity guide arm to the lid connecting rod disappears, the effect through reset spring closes sealed lid on the jar body, it is sealed with the jar mouth, it is internal to prevent that the sea water from pouring into the jar, avoid influencing the state of jar interior microorganism, this automatic culture tank that seals simple structure, low cost, do not need electromechanical control device, the use is simple, the reliability that deep sea microorganism field planting enrichment sampling device used has been improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a sectional view of the structure of the present invention;
FIG. 3 is a schematic view of the working state of the present invention;
FIG. 4 is a sectional view showing an installation state of the present invention;
FIG. 5 is a schematic view of the frame separated from the ballast mass;
FIG. 6 is a schematic view of a movable guide of embodiment 2;
FIG. 7 is a schematic view showing the state of colonization by microorganisms in example 2;
FIG. 8 is a schematic view showing a recovery state in example 2;
wherein the figures include the following reference numerals: 1. a tank body; 2. a sealing cover; 3. a cover connecting rod; 4. a can end; 41. sealing the soft cushion; 5. a return spring; 6. a lower guide block; 7. a movable guide rod; 8. an upper guide block; 9. a cover connecting block; 10. a pin shaft I; 11. a support block; 12. a pin shaft II; 13. a connecting shaft; 14. a fixing plate; 15. a frame; 151. a base plate; 16. a float material; 17. a deepwater acoustic releaser; 18. a ballast block; 19. hooking; 20. and (4) determining plants.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
Example 1
As shown in fig. 1-5, the culture tank of the present invention can be carried at the bottom of a deep sea long-term workstation and fixed on a large frame 15, besides a plurality of culture tanks, deep water acoustic releasers 17 and floating body materials 16 are also installed on the frame, the frame is fixed on a large ballast block 18, the ballast block is provided with hooks 19, the hooks are connected with the frame through the deep water acoustic releasers, and the culture tank can be placed on the sea bottom to collect the submarine microorganisms;
the deep-sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed comprises a tank body 1 and a sealing cover 2, wherein a fixed plant 20 is placed in the tank body in advance for microorganism enrichment culture, the top of the tank body is provided with a tank end cover 4, a sealing soft cushion 41 is arranged on the tank end cover 4, and the tank end cover is provided with a middle opening for collecting submarine microorganisms into the tank body; the size of the sealing cushion of the can end is matched with that of the sealing cover, the height of the sealing cushion is slightly higher than that of the can end, and the sealing cover tightly covers the opening on the can end through the sealing cushion; when collecting the microorganism, sealed lid is in the open mode, and sealed lid is elastic material such as polyurethane, strengthens sealed effect.
The sealing cover is connected with a cover connecting rod 3, one end of the cover connecting rod is hinged with the sealing cover, and the other end of the cover connecting rod is hinged with the upper guide block; the top of the sealing cover is fixed with a cover connecting block 9, the cover connecting block and the cover connecting rod are hinged together through a pin shaft I10, the sealing cover is connected to the cover connecting rod through the cover connecting block and the pin shaft, and the sealing cover can rotate around the pin shaft I. Two reset springs 5 are further installed on the outer wall of the tank body, and the other ends of the reset springs are connected with the cover connecting rod.
The cover connecting rod is L-shaped, a supporting block 11 is fixed on the upper guide block, the supporting block is hinged with one end of the cover connecting rod through a pin shaft II 12, a connecting shaft 13 is also fixed on the cover connecting rod, the other end of a return spring on the outer wall of the tank body is connected to the connecting shaft, and two ends of the connecting shaft are respectively connected with a return spring for ensuring uniform stress; under the enrichment state, reset spring is in tensile state, and reset spring is used for finishing at the microorganism collection after, through the activity of drive lid connecting rod, drives sealed lid and resets, compresses tightly sealed lid on can end cover, because the effect of sealed cushion, can seal the opening more family tightly, prevents that the sea water from gushing into.
The outer wall of the tank body is fixed with a guide block, the guide block comprises an upper guide block 8 and a lower guide block 6, guide holes are formed in the upper guide block and the lower guide block, and the guide holes are coaxial; the movable guide rod 7 penetrates through the guide holes of the upper guide block and the lower guide block, the upper end of the movable guide rod is installed in contact with the cover connecting rod, the length of the movable guide rod is determined according to the installation position of the culture tank, when the movable guide rod jacks up the cover connecting rod, the reset spring is stretched, the cover connecting rod is jacked up by the movable guide rod to drive the tank sealing cover to ascend, an opening on the tank end cover is opened, and seawater is communicated with the interior of the tank; when the movable guide rod descends, the return spring retracts, the cover connecting rod swings and descends under the action of the return spring, and the sealing cover is pressed on the end cover of the can to realize sealing.
The bottom of the tank body is provided with a fixing plate 14 for fixing the culture tank on a bottom plate 151 of the frame and fixing the culture tank on the frame.
In the microorganism enrichment stage, the lower end of the movable guide rod is supported on the bottom plate at the bottom of the frame or directly supported on the ballast block, the upper part of the movable guide rod is supported on the cover connecting rod to support the cover connecting rod, at the moment, the sealing cover is in an open state, the opening of the end cover of the can is in an open state, and at the moment, the end cover is in a microorganism planting enrichment state.
When the in-situ enrichment is finished and the culture tank is ready to rise, the deep water acoustic releaser falls off from the hook of the ballast block, the whole frame drives the culture tank and the deep water acoustic releaser to separate from the ballast block under the action of the floating body material and gradually rise, and in the rising process, because the lower end of the movable guide rod is not supported, the movable guide rod descends along the guide hole, the supporting action on the cover connecting rod disappears, the cover connecting rod falls back under the action of the reset spring to drive the sealing cover to fall back to the opening of the tank end cover, so that the sealing action is realized, the culture tank is ensured not to be communicated with external seawater in the rising process, and the sealing action is realized.
The utility model discloses a use as follows:
the marine corrosion resistant frame, the floating body material, the deep water acoustic releaser, the culture tank and the ballast block jointly form a microorganism deep sea long-term workstation for in-situ planting and culturing of submarine microorganisms, the culture tank is fixed on a bottom plate of the frame, the floating body material is used for ensuring that the buoyancy of the frame and the culture tank arranged on the frame and the like in the sea is larger than the specific gravity of the sea, the acoustic releaser is arranged in the middle of the frame, the upper part of the acoustic releaser is connected with the frame, the lower part of the acoustic releaser is provided with a unhooking device to be connected with a hook of the ballast block, the deep sea long-term workstation with the ballast block has larger self weight and can be thrown into the sea from the sea surface and freely descend to the sea bottom, when the culture tank of the seabed workstation needs to be recovered, the underwater deep water acoustic releaser is unhooked through the deck control unit of the deep water acoustic release, the other parts of the workstation losing the ballast block can freely float to the sea surface due to the buoyancy larger than the self weight, the culture tank is recovered, and the acoustic releaser part and the ballast block part are the prior art, so the working principle and the unhooking process are not detailed.
The unhooking in-process that rises, because the activity guide arm lower extreme does not support, the activity guide arm drops along the guiding hole decline, consequently disappears to the supporting role of covering the connecting rod, consequently covers the connecting rod and falls back under reset spring's effect, drives sealed lid and falls back to and cover to the end of the jar, and is sealed with the opening that covers on the end of the jar, has guaranteed the leakproofness of culture tank at deep sea workstation come-up in-process, effectively prevents that culture tank from pouring into culture tank inside at the in-process sea water that rises, destroys the enrichment state of microorganism in the culture tank.
The culture tank can be made of pollution-free corrosion-resistant materials such as titanium alloy, polytetrafluoroethylene and the like, so that the pollution influence on the microbial environment is avoided.
Example 2
As shown in FIGS. 6-8, the difference between this embodiment and embodiment 1 is that the movable guide rod between the upper guide block and the lower guide block is further provided with a section of thread, the positioning nut 71 is connected to the movable guide rod, the movable guide rod falls down during the raising of the culture tank, and due to the limiting action of the positioning nut, the positioning nut is clamped on the guide hole of the lower guide block, so that the movable guide rod does not fall to the sea bottom, but rises to the sea surface along with the culture tank, and the recycling of the device is facilitated.
In the description of the present invention, it should be noted that the terms "front end", "rear end", "left and right", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "communicating" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Of course, the above description is only a preferred embodiment of the present invention, and should not be taken as limiting the scope of the embodiments of the present invention. The present invention is not limited to the above examples, and the technical field of the present invention is equivalent to the changes and improvements made in the actual range of the present invention, which should be attributed to the patent coverage of the present invention.

Claims (8)

1. An automatically-sealable deep-sea microorganism in-situ planting enrichment culture tank comprises a tank body (1) and a sealing cover (2), and is characterized in that a guide block is fixed on the outer wall of the tank body (1), and a guide hole is formed in the guide block; a cover connecting rod (3) is connected to the sealing cover (2), one end of the cover connecting rod (3) is hinged to the sealing cover (2), and the other end of the cover connecting rod is hinged to the guide block; a movable guide rod (7) is arranged in the guide hole in a matching way, and the upper end of the movable guide rod (7) is arranged in contact with the cover connecting rod (3); reset spring (5) are still installed to jar body (1) outer wall, and reset spring (5) other end is connected with lid connecting rod (3).
2. The deep-sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that the guide block comprises an upper guide block (8) and a lower guide block (6), guide holes are formed in the upper guide block (8) and the lower guide block (6), and the guide holes are coaxial.
3. The deep sea microbe in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that the top of the tank body (1) is provided with a tank end cover (4), a sealing cushion (41) is arranged on the tank end cover (4), and the tank end cover (4) is provided with an opening in the middle.
4. The deep-sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that a cover connecting block (9) is fixed at the top of the sealing cover (2), and the cover connecting block (9) and the cover connecting rod (3) are hinged together through a pin I (10).
5. The deep-sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that the cover connecting rod (3) is L-shaped, a supporting block (11) is fixed on the upper guide block (8), and the supporting block (11) is hinged with the cover connecting rod (3) through a pin shaft II (12).
6. The deep-sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that a connecting shaft (13) is further fixed on the cover connecting rod (3), and two ends of the connecting shaft (13) are respectively connected with a return spring (5).
7. The deep-sea microorganism in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that a section of thread is arranged on a movable guide rod (7) between the upper guide block (8) and the lower guide block (6), and a positioning nut (71) is connected on the movable guide rod (7).
8. The deep-sea microbe in-situ planting enrichment culture tank capable of being automatically sealed according to claim 1, characterized in that a fixing plate (14) is arranged at the bottom of the tank body (1) and used for fixing the culture tank on a bottom plate (151) of a frame (15).
CN201921256935.0U 2019-08-05 2019-08-05 Deep sea microbe in-situ planting enrichment culture tank capable of being automatically sealed Expired - Fee Related CN210419963U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317723A (en) * 2019-08-05 2019-10-11 中国地质大学(北京) It can self-sealing Deep-Sea Microorganisms original position field planting enrichment culture tank
CN112219780A (en) * 2020-10-20 2021-01-15 中国科学院海洋研究所 Long-time-series deep-sea in-situ large-scale organism stress culture device and use method thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317723A (en) * 2019-08-05 2019-10-11 中国地质大学(北京) It can self-sealing Deep-Sea Microorganisms original position field planting enrichment culture tank
CN110317723B (en) * 2019-08-05 2023-12-19 中国地质大学(北京) Deep sea microorganism in-situ field planting enrichment culture tank capable of being automatically sealed
CN112219780A (en) * 2020-10-20 2021-01-15 中国科学院海洋研究所 Long-time-series deep-sea in-situ large-scale organism stress culture device and use method thereof
CN112219780B (en) * 2020-10-20 2023-09-12 中国科学院海洋研究所 Long-time-sequence deep-sea in-situ large-scale biological stress culture device and use method thereof

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Granted publication date: 20200428

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