CN210352751U

CN210352751U - Medical leech low-temperature sterile storage device

Info

Publication number: CN210352751U
Application number: CN201920212198.8U
Authority: CN
Inventors: 许维岸; 卢天才; 韩宏岩; 何婷婷; 韩晓阳
Original assignee: Guangxi Jinbianfei Burdock Culture Co ltd; Suzhou University
Current assignee: Guangxi Jinbianfei Burdock Culture Co ltd; Suzhou University
Priority date: 2019-02-19
Filing date: 2019-02-19
Publication date: 2020-04-21
Anticipated expiration: 2029-02-19

Abstract

The utility model discloses a medical leech low-temperature sterile storage device, which comprises a container and a container cover; the opening of the container is detachably connected with the container cover and is tightly fixed during connection; a buffer medium soaked with leech nutrient solution and antibiotics is arranged in the container; the upper surface of the container cover is sealed by a sealing device which can be torn, and the lower surface of the container cover is sealed by a microporous filtering membrane. The device provided by the utility model uses the sterile cotton soaked with the nutrient solution-antibiotic solution, not only keeps moist water environment and partial nutrition, but also keeps the sterile state of the medical leech, inhibits the pollution of mixed bacteria, and can play a role in buffering in the transportation process; the micro-porous filtering membrane on the lower surface of the container cover can isolate bacterial pollution in the air and keep the sterile state in the tube on the one hand after the tin foil paper on the upper surface of the tube cover is torn off, and on the other hand, the micro-porous filtering membrane can keep the supply of oxygen in the storage process and keep the activity of leeches.

Description

Medical leech low-temperature sterile storage device

Technical Field

The utility model relates to a medical animal container technical field, more specifically say, relate to a medical leech aseptic storage device of low temperature.

Background

The use of leeches for the treatment of diseases in humans has been known for 3000 years, and at the earliest time, leeches are used medically mainly for the following reasons: exsanguination is prevalent, while leeches can absorb blood. However, modern medicine does not have any stop in the knowledge and utilization of leeches, and it has been found that leeches can also secrete a very important natural anticoagulant hirudin which can cause continuous bleeding of wounds without coagulation, and moreover saliva contains vasodilators, which are the characteristics of which enable doctors to find leeches with high medical value. However, the medical leech is required to be higher in the process of transporting the medical leech in different places, and especially in long-distance transportation, the medical leech is always polluted and even died, and the subsequent use safety is affected.

The existing leech storage and transportation storage method is disclosed in the Chinese patent grant publication number: the invention discloses a leech seed transport box and a transport method (CN 201410620901.0). The vitality of the leeches can be kept in the transportation process, but the bacterial infection cannot be prevented, and the requirement of sterile transportation cannot be met. Therefore, the device is not suitable for storing and transporting sterile medical leeches.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a medical leech aseptic storage device of low temperature for the device can ensure the leech high survival rate in the transportation, can keep the leech to be in sterile condition again.

In order to achieve the above object, the embodiment of the present invention provides the following technical solutions:

the medical leech low-temperature sterile storage device is characterized by comprising a container and a container cover;

the opening of the container is detachably connected with the container cover and is tightly fixed during connection;

a buffer medium soaked with leech nutrient solution and antibiotics is arranged in the container;

the upper surface of the container cover is sealed by a sealing device which can be torn, and the lower surface of the container cover is sealed by a microporous filtering membrane.

The container lid accessible multiple mode realizes detachable connections with the container opening the utility model discloses in the specific implementation process, realize connection between them through threaded connection mode or buckle connected mode.

The utility model discloses in the implementation process, the leech nutrient solution is constituteed: 40mg/L of sodium chloride, 30mg/L of calcium chloride, 12mg/L of magnesium sulfate, 10mg/L of sodium bicarbonate, 0.05mg/L of zinc sulfate and water; the antibiotic is levofloxacin, the concentration is 100mg/L, and the antibiotic can be added into nutrient solution to inhibit infection of other mixed bacteria in the transportation and storage processes.

In the specific implementation process of the utility model, the buffer medium is selected from sterile cotton, and each 1g of sterile cotton is immersed in 10mL of nutrient solution (containing antibiotics), so that the cotton can be soaked but not dripped.

In the specific implementation process of the utility model, the buffer medium is arranged at the bottom of the container; the container is immersed in a buffer medium of nutrient solution and antibiotics, so that on one hand, the humidity and nutrient substances in the container can be kept, a soft soil environment is simulated, leeches are adsorbed by creeping, and a proper environment is created for leeches; on the other hand, the buffer device has a good buffer effect and avoids bumping and being damaged in the transportation process.

In the specific implementation process of the utility model, the tearable sealing device is tearable tinfoil paper, which can keep the sealing performance of the conveying pipe in the conveying process and prevent pollution in the conveying process; on the other hand, the oxygen can be directly torn off after reaching the destination, so that oxygen can enter the tube during subsequent storage; the micro-filtration membrane is an FCSF polytetrafluoroethylene micro-filtration membrane (also called a bacterial filtration membrane), after the tin foil paper on the surface of the tube cover is torn off, the tube cover can isolate bacterial pollution in the air, keep the sterile state in the tube, supply of oxygen in the storage process and be beneficial to leech breathing.

In the specific implementation process of the utility model, the container is a tubular container with an opening at one end. More specifically, the volume of the tube is more than or equal to 50mL, the diameter is more than or equal to 27.5mm, and the length is more than or equal to 103mm, so that enough oxygen required by long-distance transportation of the leech can be stored; the upper surface and the lower surface of the pipe cover are preferably 15mm in height, and the cover can be tightly covered to realize sealing and isolation to avoid bacterial pollution.

The device provided by the utility model uses the sterile cotton soaked with the nutrient solution-antibiotic solution, not only keeps moist water environment and partial nutrition, but also keeps the sterile state of the medical leech, inhibits the pollution of mixed bacteria, and can play a role in buffering in the transportation process; the micro-porous filtering membrane on the lower surface of the container cover can isolate bacterial pollution in the air and keep the sterile state in the tube on the one hand after the tin foil paper on the upper surface of the tube cover is torn off, and on the other hand, the micro-porous filtering membrane can keep the supply of oxygen in the storage process and keep the activity of leeches.

Drawings

FIG. 1 is a schematic structural view of the present invention; wherein, the left figure is a wire frame perspective view, and the right figure is a simulation figure;

FIG. 2 is a schematic view of the container lid according to the present invention; wherein, the left figure is a wire frame perspective view, and the right figure is a simulation figure.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

The embodiment of the utility model discloses aseptic storage device of medical leech low temperature makes the device can ensure the leech and be in sterile condition again in the high survival rate of transportation, can keep the leech to be in sterile condition, and under the super long distance transportation (7-10 days), the leech survival rate reaches 100%, please refer to attached figure 1 and attached figure 2.

FIG. 1 is a schematic structural diagram of the present invention, including a wire frame perspective view and a device simulation diagram; the schematic shows a container lid 1, a container 2, a buffer medium 3 soaked with leech nutrient solution and antibiotics;

in the specific implementation process, the container 2 is a tubular device with the volume of 50mL, and is a leech placing part, and a 10mL volume of buffer medium 3 (cotton) soaked in a leech nutrient solution, namely levofloxacin (100mg/L) solution is placed at the bottom of the container, so that the wet water environment and partial nutrition are kept, the sterile state of the medical leech is kept, the pollution of mixed bacteria is inhibited, and the buffer effect in the transportation process can be realized; each device can be filled with a qualified sterile leech;

fig. 2 is a schematic structural diagram of the container lid of the present invention, as shown in a wire frame perspective view and a simulation diagram, the container lid 1 is a specially-made tube lid, a layer of peelable tinfoil paper 4 is pasted on the upper surface of the container lid, and during transportation, the tinfoil paper lid keeps the transportation device in a sealed state, and keeps an aseptic state during transportation; after the container arrives at the transportation destination, the worker needs to open the container cover 1 under the aseptic condition, add 10mL of leech nutrient solution-levofloxacin solution (100mg/L), cover the container cover 1, tear off the upper surface of the container cover to tear off the tinfoil paper 4, and store the container at the low temperature of 10-15 ℃. After the tin foil paper 4 on the surface is torn off, the FCSF polytetrafluoroethylene microporous filtering membrane 5 on the lower surface of the container cover 1 isolates bacterial pollution in the air and keeps the sterile state in the tube on the one hand, and on the other hand, can keep the supply of oxygen in the storage process and keep the activity of leeches.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The medical leech low-temperature sterile storage device is characterized by comprising a container and a container cover;

cotton soaked with leech nutrient solution and antibiotics is arranged in the container;

2. The device of claim 1, wherein the removable connection is a threaded connection or a snap-fit connection.

3. The device of claim 1, wherein the peelable sealing means is a peelable foil.

4. The apparatus according to claim 1, wherein the microfiltration membrane is a FCSF polytetrafluoroethylene microfiltration membrane.

5. The apparatus of claim 1, wherein the container interior is a container bottom.

6. A device according to claim 1 or 5, wherein the container is a tubular, open-ended container.