CN210287373U - Culture apparatus for immune cell that freezes deposits - Google Patents
Culture apparatus for immune cell that freezes deposits Download PDFInfo
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- CN210287373U CN210287373U CN201920949226.4U CN201920949226U CN210287373U CN 210287373 U CN210287373 U CN 210287373U CN 201920949226 U CN201920949226 U CN 201920949226U CN 210287373 U CN210287373 U CN 210287373U
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Abstract
The utility model relates to the technical field of cell culture, and discloses a frozen immune cell culture device, which comprises an incubator, a foot stool, an upper box door, a lower box door and a hinge; a cell culture chamber is formed in the incubator, a partition board is horizontally fixed in the incubator, and the inside of the incubator is divided into an upper culture space and a lower culture space by the partition board; the upper box door and the lower box door are arranged separately and are hinged to the left side of the incubator through two hinges respectively, a cell culture space is formed after the upper box door and the upper part of the incubator are closed, a freezing immune cell culture pretreatment space is formed after the lower box door and the lower part of the incubator are closed, and a plurality of rows of vent holes are formed in the lower box door; the rear part of the inner side of the incubator is provided with a plurality of oxygen supply nozzles and carbon dioxide supply nozzles, and the rear part of the outer side of the incubator is provided with an air supply box. The utility model discloses can unfreeze, centrifugation and cultivation to the immune cell of freezing deposit simultaneously, practice thrift the space of cultivateing the room, the streamlined operation of being convenient for improves the cultivation efficiency of cell.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a culture apparatus for immune cell that freezes deposits.
Background
Cell cryopreservation is a technique for placing cells in a low-temperature environment, reducing cell metabolism and facilitating long-term storage. The cells are stored in liquid nitrogen at the temperature of-196 ℃ by using a freezing technology at a low temperature, so that the cells can be temporarily separated from a growth state and the cell characteristics can be stored, and cell recovery and culture amplification are carried out for experiments and use when needed.
The immune cells are frozen by extracting blood from a human body, separating the immune cells in the blood and then realizing long-term preservation of the immune cells by utilizing ultralow temperature. Frozen immune cells can still keep the original function after being awakened, so that the vitality of the immune cells can be kept at a young age, and the immune cells are more and more widely used in the fields of resisting aging, resisting cancers and the like.
The frozen immune cells are required to be cultured when being amplified, the conventional culture process is to firstly recover the cells, the frozen immune cells are usually thawed by using a constant-temperature water bath kettle, then a centrifugal machine is used for centrifuging, a cell suspension is left, and then the cell suspension is added with a sample medium and moved to a culture dish and put into an incubator for culture. Because current incubator function is comparatively single, each separation instrument when leading to cell culture need occupy more space, and the pipelining of being not convenient for.
SUMMERY OF THE UTILITY MODEL
In order to overcome the not enough of prior art, the utility model aims to provide a culture apparatus for immunocyte that freezes to deposit can unfreeze, centrifugation and cultivation to the immunocyte that freezes to deposit simultaneously, practices thrift the space of cultivateing the room, and the pipelining of being convenient for improves the cultivation efficiency of cell.
The utility model discloses a following technical scheme realizes: a culture device for frozen immune cells comprises an incubator, a foot stool, an upper box door, a lower box door and a hinge;
the cell culture device comprises a culture box, a baffle plate, a plurality of culture chambers and a plurality of culture chambers, wherein the culture box is in a cuboid shape, a cell culture chamber is formed in the culture box, the baffle plate is horizontally fixed in the culture box, and the baffle plate divides the interior of the culture box into an upper culture space and a lower culture space which are independent;
the plurality of foot seats are symmetrically fixed at the bottom of the incubator;
the upper box door and the lower box door are arranged separately and are hinged to the left side of the incubator through two hinges respectively, a cell culture space is formed after the upper box door and the upper part of the incubator are closed, a freezing immune cell culture pretreatment space is formed after the lower box door and the lower part of the incubator are closed, and a plurality of rows of vent holes are formed in the lower box door;
the utility model discloses a carbon dioxide incubator, including incubator, incubator outside, oxygen generator, carbon dioxide generator and PLC controller, incubator inboard rear portion is provided with a plurality of oxygen supply mouth and supplies the carbon dioxide mouth, incubator outside rear portion is provided with the air feed case, install oxygen generator, carbon dioxide generator and PLC controller in the air feed case, be connected through the breather pipe between oxygen generator's the output and the oxygen supply mouth, be connected through the breather pipe between carbon dioxide generator's the output and the carbon dioxide supply mouth, PLC controller difference oxygen generator, carbon dioxide generator pass through the wire electricity and connect.
Furthermore, a constant-temperature water bath and a centrifugal machine are arranged in the freezing immune cell culture pretreatment space in the incubator, and a circular centrifugal disc is arranged at the top of the centrifugal machine.
Further, the inside bilateral symmetry that the incubator is located cryopreserving immune cell culture preliminary treatment space is provided with two spouts, two be provided with through sliding fit in the spout and place the dish, thermostat water bath, centrifuge are fixed respectively and are placed a dish upper end both sides.
Furthermore, two guide rails are symmetrically fixed at the upper end of the partition plate, and the two guide rails are provided with object containing discs in a sliding fit manner.
Furthermore, two sliding chutes are symmetrically arranged on two sides of the inner part of the cell culture space of the incubator, a placing plate is arranged in the two sliding chutes in a sliding fit manner, the placing plate is arranged in the center of the cell culture space, a plurality of rows of ventilating through holes are arranged on the placing plate at equal intervals, and the cell culture space is divided into an upper sub-cell culture space and a lower sub-cell culture space through the placing plate.
Furthermore, a plurality of oxygen concentration sensors and carbon dioxide concentration sensors are symmetrically arranged on two sides of the interior of the cell culture space of the incubator respectively, and the oxygen concentration sensors and the carbon dioxide concentration sensors are electrically connected with the PLC through leads respectively.
Compared with the prior art, the beneficial effects of the utility model reside in that: the utility model discloses a culture apparatus for immune cell of cryopreserving, including the incubator, the foot stool, go up the chamber door, lower chamber door and hinge, the inside level of incubator is fixed with the baffle, the baffle is cut apart into upper and lower two independent culture spaces with incubator inside, the culture space on upper portion is used for unfreezing and the centrifugation of cell, the culture space of lower part is used for the cultivation of cell, go up the chamber door and separate the setting with lower chamber door, form cell culture space after closing between upper chamber door and the upper portion incubator, form cryopreserved immune cell culture pretreatment space after closing between lower chamber door and the lower part incubator, make can close the upper chamber door and carry out cell culture when carrying out unfreezing and centrifugation of immune cell in the culture space on upper portion, can close lower chamber door when the culture space of operation lower part is used for the cultivation of cell, unfreeze the immune cell of cryopreserving simultaneously through an incubator, Centrifugation and cultivation save the space of the culture chamber, are convenient for assembly line operation and improve the culture efficiency of cells.
Drawings
FIG. 1 is a schematic view showing a structure of a culture apparatus for frozen immune cells according to the present invention after being opened;
FIG. 2 is a schematic view of the full-section structure of the present invention;
fig. 3 is a left side view of the present invention.
In the figure: 2. a constant-temperature water bath kettle; 3. a centrifuge; 4. a gas supply tank; 11. an incubator; 12. a foot seat; 13. an upper box door; 14. a lower box door; 15. a hinge; 16. an oxygen concentration sensor; 17. a carbon dioxide concentration sensor; 18. an oxygen supply nozzle; 19. a carbon dioxide supply nozzle; 31. a circular centrifugal disc; 41. a chute; 42. placing a tray; 51. a partition plate; 52. a guide rail; 53. a holding tray.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.
As shown in fig. 1 to 3, the culture apparatus for cryopreserved immune cells of the present invention includes an incubator 11, a foot rest 12, an upper door 13, a lower door 14, and a hinge 15;
wherein, incubator 11 is the cuboid shape, is formed with the cell culture cavity in it, and incubator 11 inside level is fixed with baffle 51, and baffle 51 is divided into upper and lower two independent culture spaces with incubator 11 is inside, and the culture space of upper portion is used for unfreezing and the centrifugation of cell, and the culture space of lower part is used for the cultivation of cell, unfreezes, centrifugates and cultivates the immune cell of freezing stock simultaneously through an incubator 11, practices thrift the space of culture room, and the assembly line operation of being convenient for improves the cultivation efficiency of cell. A plurality of foot seats 12 are symmetrically fixed at the bottom of the incubator 11 and used for supporting the incubator 1;
The inboard rear portion of incubator 11 is provided with a plurality of oxygen suppliment mouth 18 and supplies carbon dioxide mouth 19, incubator 11 outside rear portion is provided with air supply case 4, install oxygen generator, carbon dioxide generator and PLC controller in the air supply case 4, oxygen generator is as supplying the oxygen source, be connected through the breather pipe between oxygen generator's output and the oxygen suppliment mouth 18 and be used for to supply oxygen in the incubator 11, carbon dioxide generator is as supplying the carbon dioxide source, be connected through the breather pipe between carbon dioxide generator's output and the carbon dioxide confession mouth 19 and be used for to supply carbon dioxide in the incubator 11, oxygen generator and carbon dioxide generator all use current ripe oxygen generator and carbon dioxide generator commonly used, the specific structure of the oxygen generator is not described in detail, and the PLC controller is respectively electrically connected with the oxygen generator and the carbon dioxide generator through leads for controlling the work of the oxygen generator and the carbon dioxide generator.
Be provided with constant temperature water bath 2 and centrifuge 3 in the frozen immune cell culture preliminary treatment space of depositing in incubator 11, constant temperature water bath 2 uses the present single-hole digital display formula electricity heat constant temperature water bath commonly used, its operating temperature range is 25 ~ 50 degrees centigrade, 37 degrees centigrade temperature is used commonly to unfreeze immune cell frozen stock solution, centrifuge 3 top is provided with circular centrifuge disc 31, can place a plurality of immune cell liquid pipes that freeze and deposit simultaneously on it, centrifuge 3 uses the small-size cell centrifuge commonly used, its maximum rotational speed is 5000 rpm.
Incubator 11 is located the inside bilateral symmetry that freezes and deposits immune cell culture preliminary treatment space and is provided with two spouts 41, two be provided with through sliding fit in the spout 41 and place dish 42, constant temperature water bath 2, centrifuge 3 fix respectively and place dish 42 upper end both sides, place dish 42 and can follow move the incubator 11 outside in the spout 41, are convenient for carry out the unfreezing and the centrifugal operation of cell.
Two guide rails 52 are symmetrically fixed at the upper end of the partition plate 51, and a holding tray 53 is arranged on the two guide rails 52 in a sliding fit manner and used for holding a culture dish.
The inside both sides that incubator 11 is located cell culture space also the symmetry be provided with two spouts 41, also be provided with through sliding fit in two spouts 41 and place the dish 42, should place the dish 42 and be located the central authorities in cell culture space, equidistant multirow ventilative through-hole that is provided with on it will through this dish 42 of placing the cell culture space falls into two sub cell culture spaces from top to bottom, and the ventilative through-hole that should place set up on the dish 42 can make the air between two sub cell culture spaces circulate.
The inside both sides that incubator 11 is located the cell culture space are provided with a plurality of oxygen concentration sensor 16 and carbon dioxide concentration sensor 17 respectively symmetrically, and oxygen concentration sensor 16, carbon dioxide concentration sensor 17 are connected for its acquisition signal through wire and PLC controller electricity respectively.
Example 1 as shown in fig. 1 to 3, the culture device for frozen immune cells of the present invention was used for the culture and amplification of immune cells;
taking a test tube filled with a proper amount of cell freezing solution for cell resuscitation, opening a lower box door 14, firstly operating the constant temperature water bath pot 2, adjusting the single-hole digital display type electric heating constant temperature water bath pot, controlling the water temperature of the constant temperature water bath pot at 37 ℃, and then putting the test tube into the constant temperature water bath pot 2 to be completely melted;
then, inserting the test tube into a groove on a circular centrifugal disc 31 at the top of the centrifuge 3, adjusting the centrifuge 3 to centrifuge at a commonly used rotating speed of 2000-3000 rpm, taking away the upper solution, leaving the cell sap, adding a sample medium, moving the cell sap into a culture dish, and marking the cell name and the culture date on the culture dish;
then the upper box door 13 is opened, the culture dish is placed at the upper end of the placing plate 42 or the upper end of the containing plate 53 in the incubator, the PLC controls the oxygen generator to serve as an oxygen supply source to supply oxygen into the incubator 11, the carbon dioxide generator serves as a carbon dioxide supply source to supply carbon dioxide into the incubator 11, and the oxygen concentration sensor 16 and the carbon dioxide concentration sensor 17 collect oxygen and carbon dioxide concentration signals and feed the oxygen and carbon dioxide concentration signals back to the PLC to adjust the concentrations of the oxygen and the carbon dioxide in real time.
After every placing a culture dish, can continue to carry out the cultivation of cell according to above-mentioned flow, make the utility model discloses a culture apparatus for immunocyte that freezes deposits can carry out multiunit immunocyte's culture amplification experiment simultaneously.
Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes are intended to fall within the scope of the claims.
Claims (6)
1. A culture apparatus for frozen immune cells, characterized in that: comprises a culture box (11), a foot stool (12), an upper box door (13), a lower box door (14) and a hinge (15);
the cell culture incubator (11) is in a cuboid shape, a cell culture chamber is formed in the incubator (11), a partition plate (51) is horizontally fixed in the incubator (11), and the inside of the incubator (11) is divided into an upper culture space and a lower culture space which are independent by the partition plate (51);
a plurality of foot seats (12) are symmetrically fixed at the bottom of the incubator (11);
the upper box door (13) and the lower box door (14) are arranged separately and hinged to the left side of the incubator (11) through two hinges (15), a cell culture space is formed after the upper box door (13) and the upper part incubator (11) are closed, a frozen immune cell culture pretreatment space is formed after the lower box door (14) and the lower part incubator (11) are closed, and a plurality of rows of vent holes are formed in the lower box door (14);
incubator (11) inboard rear portion is provided with a plurality of oxygen supply mouth (18) and supplies carbon dioxide mouth (19), incubator (11) outside rear portion is provided with air feed case (4), install oxygen generator, carbon dioxide generator and PLC controller in air feed case (4), be connected through the breather pipe between oxygen generator's output and the oxygen supply mouth (18), be connected through the breather pipe between carbon dioxide generator's output and the carbon dioxide supply mouth (19), PLC controller difference oxygen generator, carbon dioxide generator pass through the wire electricity and connect.
2. The culture apparatus for frozen immune cells according to claim 1, wherein: be provided with constant temperature water bath (2) and centrifuge (3) in freezing storage immune cell culture preliminary treatment space in incubator (11), centrifuge (3) top is provided with circular centrifugal plate (31).
3. The culture apparatus for frozen immune cells according to claim 2, wherein: incubator (11) are located the inside bilateral symmetry that freezes and deposits immune cell culture preliminary treatment space and are provided with two spout (41), two be provided with through sliding fit in spout (41) and place dish (42), constant temperature water bath (2), centrifuge (3) are fixed respectively and are being placed dish (42) upper end both sides.
4. The culture apparatus for frozen immune cells according to any one of claims 1 to 3, wherein: two guide rails (52) are symmetrically fixed at the upper end of the partition plate (51), and a containing disc (53) is arranged on the two guide rails (52) in a sliding fit manner.
5. The culture apparatus for frozen immune cells according to any one of claims 1 to 3, wherein: the cell culture device is characterized in that two sliding grooves (41) are symmetrically formed in two sides of the inner portion, located in a cell culture space, of the culture box (11), a placing disc (42) is arranged in each sliding groove (41) in a sliding fit mode, the placing disc (42) is located in the center of the cell culture space, multiple rows of ventilating through holes are arranged in the cell culture space at equal intervals, and the cell culture space is divided into an upper sub-cell culture space and a lower sub-cell culture space through the placing disc (42).
6. The culture apparatus for frozen immune cells according to claim 5, wherein: incubator (11) are located the inside both sides in cell culture space and are provided with a plurality of oxygen concentration sensor (16) and carbon dioxide concentration sensor (17) respectively the symmetry, oxygen concentration sensor (16), carbon dioxide concentration sensor (17) are connected with the PLC controller electricity through the wire respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920949226.4U CN210287373U (en) | 2019-06-21 | 2019-06-21 | Culture apparatus for immune cell that freezes deposits |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920949226.4U CN210287373U (en) | 2019-06-21 | 2019-06-21 | Culture apparatus for immune cell that freezes deposits |
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| Publication Number | Publication Date |
|---|---|
| CN210287373U true CN210287373U (en) | 2020-04-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920949226.4U Active CN210287373U (en) | 2019-06-21 | 2019-06-21 | Culture apparatus for immune cell that freezes deposits |
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| Country | Link |
|---|---|
| CN (1) | CN210287373U (en) |
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- 2019-06-21 CN CN201920949226.4U patent/CN210287373U/en active Active
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