CN210269584U

CN210269584U - Sample cell

Info

Publication number: CN210269584U
Application number: CN201921132702.XU
Authority: CN
Inventors: 李珍玲; 郭红莲
Original assignee: Minzu University of China
Current assignee: Minzu University of China
Priority date: 2019-07-18
Filing date: 2019-07-18
Publication date: 2020-04-07
Anticipated expiration: 2029-07-18

Abstract

The utility model relates to a check out test set technical field discloses a sample cell, include: a tank body; the first side surface of cell body is equipped with first recess, is equipped with the second recess on the tank bottom of first recess, and in the second recess was located to the sample, is equipped with the cavity that runs through the cell body on the tank bottom of second recess, and the first side surface of cell body meets with the coverslip, and the notch of first recess is covered to the coverslip, and the second side surface of cell body meets with the slide glass, and the slide glass covers the port of cavity. The utility model provides a pair of sample cell through locating the sample in the second recess, locates the second recess on the tank bottom of first recess and set up the cavity, can all form the accommodation space in the top of sample and below, both can carry out the sample and observe, can let in gas or liquid respectively again in the top of sample and below and carry out the particle screening test, and can realize the pressure measurement, can improve the functionality and the suitability of sample cell.

Description

Sample cell

Technical Field

The utility model relates to a check out test set technical field especially relates to a sample cell.

Background

The direct observation of cells or biological macromolecules by a microscope is one of the most common methods in current scientific research. At present, the most commonly used sample cell for microscopic observation of cells or biological macromolecules is prepared by a method that consists of a glass slide for bearing a sample and a cover glass for protecting the sample, and the two glass slides are usually sealed by double-sided adhesive tape or vaseline.

Such a sample cell may suffer from the following disadvantages: the pressure measurement cannot be performed; some flow cells may break and deform when given excessive pressure; the screening of micro particles cannot be carried out; the flow direction of the liquid supply can not be changed in multiple directions, so that the detection of the membrane with the chiral structure is troublesome; the observation of the same sample under different conditions, such as fluid change or administration conditions, cannot be performed; when long-time observation is carried out, the sample can be precipitated to the bottom of the pool along with the duration of time, so that the effect is influenced and the experimental result is interfered; the sample pool is not reusable, and a new sample pool is needed before each test.

The existing sample pool has the problems that the micro-particle screening cannot be carried out and the pressure measurement is inconvenient.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model aims at providing a sample cell for solve or partially solve current sample cell and exist and can not carry out the screening of small particle and the pressure measurement's of being not convenient for problem.

(II) technical scheme

In order to solve the technical problem, the utility model provides a sample cell, include: a tank body; the utility model discloses a cell body, including cell body, cover glass, cell body, cover glass, the first side surface of cell body is equipped with first recess, be equipped with the second recess on the tank bottom of first recess, the sample is located in the second recess, be equipped with on the tank bottom of second recess and run through the cavity of cell body, the first side surface of cell body meets with the cover glass, the cover glass covers the notch of first recess, the second side surface of cell body meets with the slide glass, the slide glass covers the port of cavity.

On the basis of the scheme, third grooves are arranged on the bottoms of the first grooves and one side or two sides of the second grooves, and the third grooves are independent from the cavity.

On the basis of the scheme, the device also comprises a first channel, a second channel, a third channel and a fourth channel which are arranged in the tank body and are mutually independent; the first channel and the second channel are respectively communicated with the outside of the tank body and the third groove, and the third channel and the fourth channel are respectively communicated with the outside of the tank body and the cavity.

On the basis of the scheme, the first channel is connected with a first inlet piece on the surface of the tank body, and the second channel is connected with a first outlet piece on the surface of the tank body; the third channel is connected with the second inlet part on the surface of the tank body, and the fourth channel is connected with the second outlet part on the surface of the tank body.

On the basis of the scheme, the first inlet part, the first outlet part, the second inlet part and the second outlet part are respectively in a gradually expanding shape, and the section size of one end connected with the surface of the tank body is larger.

On the basis of the scheme, the cavity is in a gradually expanding shape from the groove bottom of the second groove to the second side surface of the tank body.

On the basis of the scheme, the first channel or the second channel is connected with a peristaltic pump or an injector outside the tank body; the third channel or the fourth channel is connected with a peristaltic pump or an injector outside the tank body.

On the basis of the scheme, the cover glass is connected with the first side surface of the cell body in a sealing mode, and the glass slide is connected with the second side surface of the cell body in a sealing mode.

On the basis of the above scheme, the depth of the first groove is as follows: 0.5-2 mm; the depth of the second groove is as follows: 0.1-0.3 mm.

On the basis of the scheme, the pool body comprises a 3D printing piece.

(III) advantageous effects

The utility model provides a pair of sample cell through locating the sample in the second recess, locates the second recess on the tank bottom of first recess and set up the cavity, can all form the accommodation space in the top of sample and below, both can carry out the sample and observe, can let in gas or liquid respectively again in the top of sample and below and carry out the particle screening test, and can realize the pressure measurement, can improve the functionality and the suitability of sample cell.

Drawings

Fig. 1 is a schematic structural diagram of a sample cell according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of the connection of the channels according to the embodiment of the present invention;

fig. 3 is an overall schematic diagram of a sample cell according to an embodiment of the present invention.

Description of reference numerals:

1-pool body; 2-a first groove; 3-a second groove;

4, a cavity; 5-a third groove; 6-first channel;

7 — a second channel; 8-a third channel; 9 — a fourth channel;

10-a first inlet piece; 11 — a first outlet member; 12-a second inlet piece;

13-second outlet member.

Detailed Description

The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The embodiment of the utility model provides a sample cell can be arranged in the microscope to observe and bear the sample in the detection. Referring to fig. 1, the sample cell includes: a tank body 1; the first side surface of cell body 1 is equipped with first recess 2. The bottom of the first groove 2 is provided with a second groove 3, and the sample is arranged in the second groove 3. The bottom of the second groove 3 is provided with a cavity 4 which penetrates through the tank body 1. Namely, one end port of the cavity 4 is positioned at the bottom of the second groove 3, and the other end port is positioned at the second side surface of the tank body 1. The cavity 4 can be communicated with the outside of the tank body 1 and the second groove 3.

The first side surface of the tank body 1 is connected with a cover glass, and the cover glass covers the notch of the first groove 2. Since the sample is placed in the second recess 3, the second recess 3 is arranged at the bottom of the first recess 2 such that there is a gap between the sample and the cover glass, into which gas or liquid can be introduced. The second side surface of the tank body 1 is connected with a glass slide which covers the port of the cavity 4. The gap between the sample and the cover glass is positioned above the sample, the cavity 4 is positioned below the sample, and gas or liquid can be introduced into the cavity.

Furthermore, the sample is arranged in the second groove 3, and has a certain accommodating space with intervals between the cover glass and the glass slide, so that a pressure intensity detection device can be conveniently arranged, and the pressure intensity can be measured.

The sample cell that this embodiment provided, through in locating second recess 3 with the sample, locate second recess 3 on the tank bottom of first recess 2 and set up cavity 4, can all form the accommodation space in the top and the below of sample, both can carry out the sample observation, can let in gas or liquid respectively again in the top and the below of sample and carry out the particle screening test, and can realize the pressure measurement, can improve the functional and the suitability of sample cell.

On the basis of the above embodiment, further, referring to fig. 1 and 2, the bottom of the first groove 2 and one side or two sides of the second groove 3 are provided with third grooves 5, and the third grooves 5 are independent from the cavity 4. The notch of the third groove 5 communicates with the notch of the second groove 3. I.e. the interior of the third recess 5 is in communication with the space above the sample in the second recess 3. The space of the gap above the sample can be increased, and gas or liquid can be stored conveniently.

On the basis of the above embodiment, further, referring to fig. 2, a sample cell further includes a first channel 6, a second channel 7, a third channel 8 and a fourth channel 9 which are arranged inside the cell body 1 and are independent from each other; the first channel 6 and the second channel 7 are respectively communicated with the outside of the tank body 1 and the third groove 5, and the third channel 8 and the fourth channel 9 are respectively communicated with the outside of the tank body 1 and the cavity 4.

On the basis of the above embodiment, further, the first channel 6 is connected with the first inlet member 10 at the surface of the tank body 1, and the second channel 7 is connected with the first outlet member 11 at the surface of the tank body 1; the third channel 8 is connected to a second inlet piece 12 at the surface of the tank 1 and the fourth channel 9 is connected to a second outlet piece 13 at the surface of the tank 1.

The first channel 6 is connected at one end to the first inlet piece 10 and at the other end opens into the third recess 5. One end of the second channel 7 is connected to the third groove 5 and the other end is connected to the first outlet member 11. Gas or liquid may be introduced into the third recess 5 through the first passage 6. The gas or liquid in the third recess 5 can flow through the slot into the second recess 3 to contact the sample. The gas or liquid in the third groove 5 can be discharged through the second channel 7.

The third channel 8 is connected at one end to the second inlet piece 12 and at the other end opens into the cavity 4. One end of the fourth channel 9 is connected to the cavity 4 and the other end is connected to the second outlet member 13. Gas or liquid can be introduced into the cavity 4 through the third channel 8. The gas or liquid in the cavity 4 is in contact with the sample at the bottom of the second recess 3. The gas or liquid in the cavity 4 can be discharged through the fourth channel 9.

Through each channel, gas or liquid can be conveniently and independently introduced into or discharged from the space above and below the sample. The liquid changing and dosing in the space above and below the sample can be carried out in closed use, and the continuous liquid changing or dosing in observation can be realized, so that the sample pool has more functionality and is suitable for more situations.

On the basis of the above embodiment, further, the first inlet member 10, the first outlet member 11, the second inlet member 12 and the second outlet member 13 are respectively divergent, and the cross-sectional size of one end connected with the surface of the tank body 1 is larger. That is, the cross section of one end of the first inlet part 10, the first outlet part 11, the second inlet part 12 and the second outlet part 13 connected with the surface of the tank body 1 is larger than that of the other end, which is convenient for the connection with the external pipeline and is suitable for more pipelines with different specifications.

On the basis of the above embodiment, further, the cavity 4 is divergent from the bottom of the second groove 3 to the second side surface of the tank body 1. Since the second recess 3 is used for placing a sample and has a small size, the cavity 4 is tapered, so that the volume of the cavity 4 can be increased, which facilitates connection with the third channel 8 and the fourth channel 9, and the storage space of the cavity 4 can be increased.

On the basis of the above embodiment, further, the first channel 6 or the second channel 7 is connected with a peristaltic pump or a syringe outside the cell body 1; the third channel 8 or the fourth channel 9 is connected with a peristaltic pump or a syringe outside the tank body 1. A peristaltic pump and syringe are used to drive the liquid delivery into the third recess 5 or cavity 4. A peristaltic pump or syringe may be connected to the inlet or outlet member by tubing.

On the basis of the above embodiment, further, the cover glass is hermetically connected with the first side surface of the cell body 1, and the glass slide is hermetically connected with the second side surface of the cell body 1. The two glass slides and the tank body 1 can be hermetically connected through Vaseline, and then the closed flowing sample tank can be realized.

On the basis of the above embodiment, further, the depth of the first groove 2 is: 0.5-2 mm; the depth of the second groove 3 is: 0.1-0.3 mm. The size can meet the use requirement.

On the basis of the above described embodiment, further, the cell body 1 comprises 3D prints. The drawing design can be carried out on solidworks software firstly, and then 3D printing is adopted to manufacture the drawing.

On the basis of the above embodiments, further, the present embodiment provides a multifunctional sample cell for laboratory detection, especially suitable for performing fine particle screening, measuring pressure, detecting the property of the permeable membrane with chiral structure, and observing the sample under an optical microscope for a longer time. Referring to fig. 3, the whole body of the sample cell 1 may be a cuboid with a length, a width and a height of 40-60mm, 20-30mm and 2-6mm in sequence. The left end and the right end are respectively provided with two circular truncated cones with the length of 4-6mm, the diameter of the upper plane of 2-3mm and the diameter of the lower plane of 3-4mm, the lower planes of the circular truncated cones are connected with the surface of the tank body 1, and the circular truncated cones are an inlet part and an outlet part.

The center of the upper surface of the tank body 1 is provided with a rectangular channel which has the length and the width of 10-18mm and 5-15mm in sequence and penetrates through the tank body 1, and a ladder platform with the upper surface being a square with the side length of 8-12mm, the height of the bevel edge being 3.2mm and the lower surface being superposed with the lower surface of the tank body 1 is arranged in the rectangular channel. Namely, the lower surface of the step platform and the lower surface of the tank body 1 are positioned on the same plane and are filled with the end part of the rectangular channel. So that no gap is left between the lower surface of the step and the rectangular channel. And a third groove 5 is formed between the inclined planes at the two sides of the step and the inner wall of the rectangular channel. The upper surface of the step is the bottom of the first groove 2 and is slightly lower than the surface of the tank body 1, so that the liquid in the third groove 5 can flow into the second groove 3 conveniently.

The center of the upper surface of the ladder platform is recessed by 0.1-0.3mm to form a square groove with the side length of 3-3.4mm, namely a second groove 3, so that a sample to be observed or a permeable membrane can be placed conveniently. The bottom of the second groove 3 is hollowed into a truncated cone-shaped cavity 4 with the diameter of 1.5-1.7mm on the upper surface and the diameter of 3-5mm on the lower surface. The center of each round table on the left side and the right side of the sample cell is provided with a hollow channel with the diameter of 1-2mm, and the hollow channel is communicated with a third groove 5 or a cavity 4 to form a liquid feeding channel. Wherein the first and second channels 7 communicate with the third recess 5, i.e. the space on both sides of the landing, for feeding liquid from above the second recess 3. The third and fourth channels 9 communicate with the cavity 4 below the second recess 3 and feed liquid from below the second recess 3. When the device is used, a glass slide is placed in the cavity 4 below the sample cell, a cover glass is placed in the rectangular channel above the sample cell, and the space between the two glass slides and the cell body 1 is sealed by vaseline, so that a closed flowing sample cell can be realized.

The use process of the sample cell specifically comprises the following steps: the first, second, third and fourth channels are respectively fixedly connected with the four silicone tubes through the inlet piece or the outlet piece. And a permeable membrane with proper size is arranged at the second groove 3. Distilled water is introduced into the third channel 8, sample liquid containing particles to be screened is introduced into the first channel 6, pressure applied to two sides of the permeable membrane is different by changing flow rates of the liquid introduced into the first channel 6 and the third channel 8, opening degrees of pores of the permeable membrane are different, and then the required particles are screened out. And waste liquid is collected at the 7-port of the second channel, and screened particles are collected at the 9-port of the fourth channel.

If the permeable membrane has a chiral structure, the silica gel tube at the openings of the second channel 7 and the fourth channel 9 can be clamped by a water stop clamp, so that liquid can only flow in from the first channel 6 or the third channel 8 to determine whether the liquid flows through the permeable membrane from the front side or the back side of the permeable membrane. And fixing the sample cell on a sample stage of a microscope, and observing. The liquid in the third channel 8 flows through the lower part of the second groove 3 for placing the sample, and the liquid in the first channel 6 and the second channel 7 is independent from the liquid in the third channel 8 and the fourth channel 9, so that the interference of the liquid feeding to the sample is avoided; and because the sample both sides all have liquid to support it, also can not have a large amount of samples to deposit in the sample cell when observing for a long time, reduce the background and disturb.

The embodiment provides a multifunctional sample cell which can be introduced with gas or liquid for particle screening and pressure measurement, can be used in a closed manner, can be used for changing liquid in the sample cell and feeding medicine, and can continuously change the liquid or feed medicine during observation. The sample cell consists of a cell body 1 with two liquid feeding inlets, two outlets and a sample fixing groove, a glass slide, a cover glass, a peristaltic pump, an injector, a silicone tube and the like which are matched for use. The sample cell has the advantages of reasonable structure, small volume, simple manufacture, repeated use, compatibility with various optical microscopes, convenient replacement of injected liquid and gas and easy control of liquid and gas flow rate.

The sample cell provided by the embodiment has multiple functions, and can be used for observing a sample, screening particles, measuring pressure and the like; the membrane is suitable for detecting the membrane with the chiral structure, and the unique flow path design of the membrane ensures that the flow direction of the liquid supply can be changed very conveniently in the same environment, and the liquid supply direction can be changed in multiple directions; the smaller cavity area reduces the sample consumption, reduces the time for searching a specific sample, and also reduces the bottom of a sample pool for depositing a large amount of samples; the operation area is separated from the liquid feeding channel, so that the interference of surrounding samples can be effectively avoided; the micro-motion platform driven by various electric drives or piezoelectric ceramics is easy to be compatible; the microscope is easy to be compatible for use with various microscopes; small, adopt 3D to print the preparation, processing is simple, and the function is many, convenient to use.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sample cell, comprising: a tank body; the utility model discloses a cell body, including cell body, cover glass, cell body, cover glass, the first side surface of cell body is equipped with first recess, be equipped with the second recess on the tank bottom of first recess, the sample is located in the second recess, be equipped with on the tank bottom of second recess and run through the cavity of cell body, the first side surface of cell body meets with the cover glass, the cover glass covers the notch of first recess, the second side surface of cell body meets with the slide glass, the slide glass covers the port of cavity.

2. The cuvette according to claim 1, wherein a third groove is provided on the bottom of the first groove and on one or both sides of the second groove, and the third groove is independent of the cavity.

3. The sample cell according to claim 2, further comprising a first channel, a second channel, a third channel and a fourth channel which are independent from each other and are arranged inside the cell body; the first channel and the second channel are respectively communicated with the outside of the tank body and the third groove, and the third channel and the fourth channel are respectively communicated with the outside of the tank body and the cavity.

4. A sample cell according to claim 3, wherein the first passage is connected to a first inlet member at a surface of the cell body and the second passage is connected to a first outlet member at a surface of the cell body; the third channel is connected with the second inlet part on the surface of the tank body, and the fourth channel is connected with the second outlet part on the surface of the tank body.

5. The sample cell according to claim 4, wherein the first inlet member, the first outlet member, the second inlet member and the second outlet member are respectively divergent and have a larger cross-sectional size at an end connected with the surface of the cell body.

6. The sample cell according to any one of claims 1 to 5, wherein the cavity is divergent from a bottom of the second groove to the second side surface of the cell body.

7. A sample cell according to claim 3, wherein the first channel or the second channel is connected to a peristaltic pump or a syringe outside the cell body; the third channel or the fourth channel is connected with a peristaltic pump or an injector outside the tank body.

8. The sample cell of any one of claims 1 to 5, wherein the cover glass is sealingly connected to a first side surface of the cell body, and the slide glass is sealingly connected to a second side surface of the cell body.

9. A cuvette as claimed in any one of claims 1 to 5, wherein the depth of the first recess is: 0.5-2 mm; the depth of the second groove is as follows: 0.1-0.3 mm.

10. A sample cell according to any of claims 1 to 5, wherein the cell body comprises a 3D print.