CN2102296U - Program controlled automatic circulation instrument for polymerase chain reaction - Google Patents

Abstract

The utility model relates to a medical instrument which is also called to a PCR programmed control automatic circulation instrument. The PCR technique is a natural replication process which simulates DNA (deoxyribonucleic acids). A specimen pool of the utility model adopts a nonisothermal three-pool structure arranged in parallel, and thus, the specimen pool and a freezing bath groove can be additionally arranged optionally. The utility model adopts the method that a mechanical arm is driven by double motors controlled by a computer to move a specimen disk, the mechanical transmission structure of the original device is greatly simplified, and the heat exchanging and the pool exchanging of the specimen are reliable and quick. A single aqueous medium is changed into double mediums, namely, a heat insulating layer which can prevent water content from evaporating is additionally arranged on the water surface, and the problem of oversized space humidity of the instrument is avoided. The utility model has the advantages of complete function and reliable performance, and the utility model can be easily refitted to an automatic piece coloring instrument.

Description

Program controlled automatic circulation instrument for polymerase chain reaction

The utility model belongs to medical apparatus.Polymerase chain reaction,PCR (abbreviation round pcr) or thymus nucleic acid (being called for short DNA) amplification technique is a kind of DNA analysis technology that the mid-80 is set up.The natural reproduction process of its analog D NA is carried out massive duplication at external use physics and biochemical method to dna fragmentation, thereby easily characteristic fragment is carried out check and analysis, and gene analysis technique is greatly simplified.

Existing polymerase chain reaction,PCR circulation instrument sample pond is fan-shaped array three pool structures, shifts specimen disc with the complicated mechanical transmission mechanism, and the medium that soaks sample is that single water is medium.This instrument can not increase sample pond and freezing bath owing to sample pond fan-shaped array, mechanism's complexity of mobile specimen disc, and transport velocity is slow.Do not have water conservation, the thermal insulation layer that prevents water evaporates in specimen disc, this construction and device changes the temperature time long (one fen half) and causes enzyme deactivation easily when having caused the work of circulation instrument, and by product increases, troublesome poeration, shortcomings such as the water evaporates loss is big, and humidity is excessive in the instrument, and experimental period is oversize.

The purpose of this utility model is in order to overcome the above-mentioned shortcoming of existing polymerase chain reaction,PCR circulation instrument, to have designed " polymerase chain reaction,PCR PROGRAMMED AUTO circulation instrument ".

The design's major technique content: because of the committed step of round pcr is sex change, renaturation, three steps of extension, each step finishes under different temperature and time conditions.The design is sample pond (1), (2), (8) of dipping bath sample not isothermal three pool structures that are designed to be arranged in parallel, and sample pond size is long 10-14cm, wide 6-10cm, 5-7cm deeply.Each pond temperature is respectively 90-95 ℃, 50-70 ℃, 70-78 ℃.The changing the pond, change that level is made in bi-motor (5), (6) that temperature computerizeds control, movement in vertical direction moves specimen disc of sample pond, two of the stepper-motors of motor usefulness 20-50W, change temperature adjacent two pond time swap≤4 of time second, (8) change to time≤6, pond (1) second from the pond.In order to prevent water evaporates in the sample pond, in the pond, increase one deck water conservation, thermal insulation layer (9), this layer liquid is that boiling point height, steam force down, proportion less than water, with the immiscible liquid of water, as paraffin, mineral wet goods.Make sample dipping bath in two media.This insulation layer thickness is 1-5mm.Any direction in the sample pond can increase the sample pond and set up the freezing bath with refrigerating apparatus.

The design's advantage and positively effect: because not isothermal three pool structures that are arranged in parallel have been adopted in the sample pond, can arbitrarily increase sample pond and freezing bath when making instrument, increased dipping bath sample number of packages and the cold storage of temporary sample chamber, brought great convenience to the experimenter.Simultaneously the sample pond is easy to change into and dyes the sheet pond, each sample pond one is divided into two, each pond is reagent such as splendid attire staining fluid, stationary liquid, elutriant respectively, this instrument just become have six dye the sheet pond dye the sheet instrument automatically, appropriate change software, control partly can be moved as required and be moved part to be dyed, and makes a tractor serves several purposes.Adopt the computer-controlled bi-motor to drive that mechanical arm is made level, movement in vertical direction moves specimen disc, greatly simplified the complex mechanical structure that original equipment moves specimen disc, and specimen disc has changed temperature, changed the pond accurately and reliably, fast.Change temperature and the time shorten to 4-6 second, shortcoming such as overcome that experimental period is long, enzyme deactivation and by product increase by original one minute half.Adopting two media is to increase the protective layer that one deck prevents water evaporates on the water surface, obviously plays preventing that humidity is excessive in water evaporates, the instrument, pollutes the effect of instrument, the troublesome operations of having exempted frequent moisturizing.The temperature in each sample pond also is easier to control.The design " polymerase chain reaction,PCR PROGRAMMED AUTO circulation instrument " greatly simplified gene analysis technique, application prospect is extensive in molecular biology, life science, for the clinical diagnostic means fast and accurately that provides round pcr to use, increased effective diagnostic method.This instrumental function is complete, dependable performance, and a tractor serves several purposes, cost is low, has fabulous social benefit and economic benefit.

Accompanying drawing and drawing explanation:

Fig. 1 instrument structure that circulates is overlooked

Fig. 2 sample pond section

Sequence number among the figure (1), (2), (8) sample pond, (3), (4) slide rail, (5) motor vertical, (6) horizontal motor, (7) specimen disc, (9) paraffin layer (or mineral oil reservoir), (10) water layer, (11) freezing tank.

Embodiment: the design's sample pond adopts long 12cm, wide 8cm, dark 6cm three ponds to be arranged in parallel, and each pond temperature is respectively 94 ℃, 60 ℃, 78 ℃.Change temperature adjacent two pond of time time≤4 second, from 3 ponds to time≤6,1 pond second.Sample pond medium is that water layer 4.8cm is thick below, is that liquid paraffin layer 2mm is thick above.Make level respectively and movement in vertical direction moves specimen disc with two of 24W stepper-motors.

Claims (3)

1, not isothermal three pool structures that are arranged in parallel are adopted in a kind of polymerase chain reaction,PCR circulation instrument it is characterized in that circulating instrument sample pond (1), (2), (8).

2, it is characterized in that circulating by the said circulation instrument of claim 1 and soak the sample medium in the instrument sample pond for two media, adopt water layer (10) and liquid paraffin or (9) two kinds of liquid of mineral oil reservoir, liquid paraffin on bubbling through the water column or mineral oil layer thickness are 1 to 5mm.

3, it is characterized in that being provided with freezing bath (11) by claim 1,2 said circulation instrument.