CN2697102Y - Liquid flowing reaction constent-temp. box for PCR augmentor - Google Patents
Liquid flowing reaction constent-temp. box for PCR augmentor Download PDFInfo
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- CN2697102Y CN2697102Y CN 200420044241 CN200420044241U CN2697102Y CN 2697102 Y CN2697102 Y CN 2697102Y CN 200420044241 CN200420044241 CN 200420044241 CN 200420044241 U CN200420044241 U CN 200420044241U CN 2697102 Y CN2697102 Y CN 2697102Y
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- constant temperature
- thermostatic bath
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Abstract
The utility model discloses a liquid flowing reaction constant temperature box for a PCR augmentor. The utility model comprises a constant temperature tank, a support column, a reaction tube, and thermal insulating plates, wherein, the support column is arranged at the center of the constant temperature reaction tank, and the reaction tube uniformly winds around the thermal insulating column surface of the support column in a helical shape; The surrounding zone of the support column in the constant temperature tank is divided into three constant temperature reaction zones with different temperatures through three thermal insulating plates; the positions of the thermal insulating plates in the constant temperature tank, which are corresponding to the reaction tube, are provided with grooves through which the reaction tube penetrates; reaction liquid flows in the reaction tube at a uniform speed; according to the entering sequence of the reaction tube, the three reaction zones are respectively a high-temperature denaturing reaction zone, a low temperature annealing reaction zone, and a moderate temperature extending constant temperature reaction zone. The utility model has the advantages of no mechanical operation, rapid temperature change, stable temperature control, continuous circulation flowing heating, miniaturization amplification, large-scale amplification, increased amplification efficiency, and increased price ratio for the PCR augmentor.
Description
Technical field
The utility model relates to a kind of conversion unit that is used for the pcr amplification instrument, relates in particular to a kind of liquid-flow reaction thermostat container that is used for the pcr amplification instrument.
Background technology
Polymerase chain reaction (Polymerase Chain Reaction, PCR) be the external nucleic acid amplification technologies that the mid-80 grows up, have special, responsive, productive rate is high, quick, easy, good reproducibility, easy outstanding advantage such as automatization, can in vitro goal gene or a certain dna fragmentation that will study be expanded to 100,000 and even 1,000,000 times in a few hours at one, make naked eyes energy direct viewing and judgement.Can from a hair, bleed in addition cell amplify capacity DNA for analysis and research with detect and identify.The thing that several weeks of days past just can accomplish just can be finished with PCR in several hours, and round pcr is revolutionary invention and the milestone in the biomedical sector for this reason.
PCR reaction three step circulations, each circulation are through three constant temperature stages: high-temperature denatured, low-temperature annealing, the extension of middle temperature.Can be through about 30 circulations DNA with index amplification doubly.
Present DNA cloning instrument mainly contains alternating temperature heating and the hot dual mode of thermostatic bath.
Silicon heating during alternating temperature is heated to be, this is to use the most general a kind of, has easy realization automatization, fast, the direct heating cooling of speed, good effectiveness, but weak point is: 1. cost is higher; 2. the medium of Shi Yonging is a metal, though heat conduction is good, is difficult to accomplish seamless the contact with reaction tubes; 3. owing to be the bottom heating, bottom pressure is bigger, and the possibility of overflowing is arranged.
Thermostatically heating is based on water-bath and oil bath, their specific heat big (oil ratio water is bigger), solvation object, contact with reactant is seamless, in the widely used various thermal cyclers, the result of use of the thermal cycler of water-bath type is best in China medical institutions laboratory.And existing water-bath type thermal cycler is under the manipulation of mechanical arm, the test tube that fills the DNA sample is finished switching process from a kind of thermostatic bath of temperature to another kind of temperature thermostatic bath quickly, rapid and accurate to guarantee the temperature variation in the test tube, the validity of assurance polymerase chain reaction.The temperature of water is a constant in 3 thermostatic baths of this water-bath type thermal cycler, can be in advance sets according to the requirement of polymerase chain reaction, and therefore variation of temperature depends on the translational speed of mechanical arm between two step of reaction.The temperature variation of enzyme is very fast in the test tube, can reach per second and change 10 ℃, the thermal cycler of other types then must be readjusted temperature in the temperature transition process, and the temperature of enzyme changes slower relatively in the test tube, per second only changes 1～2 ℃ or lower, and enzyme is lost activity.Their weak point is: the device structure complexity, and schedule of operation is many, and the possibility of operate miss is big, and level of automation is low, and the failure rate of machinery height is difficult to miniaturization.
The utility model content
The purpose of this utility model is to provide a kind of liquid-flow reaction thermostat container that is used for the pcr amplification instrument, and the pcr amplification instrument can be increased in miniaturization, and the amplification of maximizing again can improve the efficient of amplification and the cost performance of pcr amplification instrument.
The purpose of this utility model is achieved by the following technical programs: comprise thermostatic bath, support column, reaction tubes, thermal baffle, described support column is arranged on the center of isothermal reaction groove, reaction tubes in the shape of a spiral shape be wrapped in equably support column can heat insulation cylinder on; Zone around the thermostatic bath internal support column all is divided into three isothermal reaction districts that are differing temps by three thermal baffles, and the position of corresponding reaction tubes is provided with the groove that reaction tubes is passed through on the thermal baffle in the thermostatic bath.Reaction solution can be at the uniform velocity mobile in reaction tubes, the sequencing that enters around the support column cylinder by reaction tubes, above-mentioned three reaction zones are respectively high-temperature denatured isothermal reaction district, low-temperature annealing isothermal reaction district and middle temperature and extend the isothermal reaction district, the utility model need not power operation, and the reaction solution in the reaction tubes passes through each isothermal reaction district automatically.
For guaranteeing the constant temperature effect in each isothermal reaction zone, be provided with sealing-ring between groove on the thermal baffle described in the utility model and the reaction tubes, produce the infiltration phenomenon when passing different isothermal reactions zone to prevent reaction tubes.
Thermostatic bath described in the utility model and support column can be the equilateral triangle column type, both seamed edge correspondences, and described thermal baffle is arranged between the seamed edge of thermostatic bath and support column; Thermostatic bath and support column also can be cylinder shape.The shape and the size of thermostatic bath and support column can be set according to practical situation.
The utlity model has following beneficial effect:
(1) need not power operation, quick changeable temperature and circulate heating continuously automatically, temperature control is stable.
(2) simple in structure, easy to operate, can miniaturization increase, the amplification of maximizing again can improve the efficient of amplification
(3) cost is low, the cost performance height of pcr amplification instrument.
Description of drawings
Below in conjunction with embodiment and accompanying drawing the utility model is described in further detail:
Fig. 1 is the structural representation of the utility model embodiment;
Fig. 2 is that reaction tubes is wrapped in synoptic diagram on the support column cylinder among the utility model embodiment;
Fig. 3 is the synoptic diagram in single isothermal reaction district among the utility model embodiment;
Fig. 4 is the vertical view of Fig. 1.
Embodiment
Fig. 1～Figure 4 shows that embodiment of the present utility model comprises thermostatic bath 1, support column 2, reaction tubes 3, thermal baffle 4, heating unit 5 and temperature sensor 6.The cylinder of thermostatic bath 1, support column 2 and thermal baffle 4 must be heat insulation, and lagging material can be with rubber plastic etc.
Thermostatic bath 1 and support column 2 are the equilateral triangle column type that varies in size, and support column 2 is arranged on the center of thermostatic bath 1, and both seamed edge correspondences.Reaction tubes 3 in the shape of a spiral shape be wrapped in equably support column 2 can heat insulation cylinder on, be wound with 30 circles altogether, the diameter of reaction tubes 3 is bigger slightly than common capillary reaction, but can not be too big, prevents to heat inhomogeneous, reaction solution is at the uniform velocity mobile in reaction tubes 3.
Zone around thermostatic bath 1 internal support column 2 is divided into three reaction zones by three thermal baffles 4, and thermostatic bath 1 and thermal baffle 4 connect sealing and fixing in mode for convenience detach.Thermal baffle 4 is located at respectively between the seamed edge of thermostatic bath 1 and support column 2.The position of corresponding reaction tubes 3 is provided with the groove that reaction tubes is passed through on the thermal baffle 4, is provided with seal washer between this groove and the reaction tubes.By the sequencing that reaction tubes 3 enters around support column 2 cylinders, above-mentioned three reaction zones are respectively high-temperature denatured isothermal reaction district 7, low-temperature annealing isothermal reaction district 8 and middle temperature and extend isothermal reaction district 9.Each isothermal reaction district adopts constant temperature water bath, realizes the thermostatic control of differing temps by heating unit 5, temperature sensor 6 and thermometer are set; The material of heating unit 5 can be a metal, and thermometer is selected 0～100 degree.
The ratio of reaction tubes 3 residence time in three isothermal reaction districts designs according to actual needs, and the circumscribed circle with support column 2 cylinders when design is the axle center, adjusts the reaction times proportional distribution according to the pairing central angle in isothermal reaction district.
In addition, thermostatic bath 1 and support column 2 also can be the cylinder shape (not shown)s that varies in size.
Claims (4)
1, a kind of liquid-flow reaction thermostat container that is used for the pcr amplification instrument, it is characterized in that: comprise thermostatic bath, support column, reaction tubes and thermal baffle, described support column is arranged on the center of isothermal reaction groove, reaction tubes in the shape of a spiral shape be wrapped in equably support column can heat insulation cylinder on; Zone around the thermostatic bath internal support column all is divided into three isothermal reaction districts that are differing temps by three thermal baffles, and the position of corresponding reaction tubes is provided with the groove that reaction tubes is passed on the thermal baffle in the thermostatic bath.
2, the liquid-flow reaction thermostat container that is used for the pcr amplification instrument according to claim 1 is characterized in that: be provided with sealing-ring between groove on the described thermal baffle and the reaction tubes.
3, the liquid-flow reaction thermostat container that is used for the pcr amplification instrument according to claim 1 and 2, it is characterized in that: described thermostatic bath and support column are the triangle column type, both seamed edge correspondences, described thermal baffle is arranged between the seamed edge of thermostatic bath and support column.
4, the liquid-flow reaction thermostat container that is used for the pcr amplification instrument according to claim 1 and 2, it is characterized in that: described thermostatic bath and support column are cylinder shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420044241 CN2697102Y (en) | 2004-04-01 | 2004-04-01 | Liquid flowing reaction constent-temp. box for PCR augmentor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420044241 CN2697102Y (en) | 2004-04-01 | 2004-04-01 | Liquid flowing reaction constent-temp. box for PCR augmentor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2697102Y true CN2697102Y (en) | 2005-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420044241 Expired - Fee Related CN2697102Y (en) | 2004-04-01 | 2004-04-01 | Liquid flowing reaction constent-temp. box for PCR augmentor |
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| CN (1) | CN2697102Y (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363001B (en) * | 2008-08-22 | 2012-06-27 | 金银杏生物科技(北京)有限公司 | Sliding mode heat transfer media plate PCR instrument |
| CN101287845B (en) * | 2005-05-11 | 2012-07-18 | 先进液体逻辑公司 | Method and device for conducting biochemical or chemical reactions at multiple temperatures |
| CN103911283A (en) * | 2014-03-18 | 2014-07-09 | 北京工业大学 | Spiral tri-prism PCR micro-fluidic apparatus |
| CN105602842A (en) * | 2016-01-14 | 2016-05-25 | 江苏猎阵生物科技有限公司 | One-click nucleic acid amplification instrument and application thereof |
| CN107446811A (en) * | 2017-07-25 | 2017-12-08 | 新疆昆泰锐生物技术有限公司 | A kind of reaction instrument for PCR tubular type temperature regulating device and comprising the device |
| CN109022263A (en) * | 2017-06-08 | 2018-12-18 | 昆泰锐（武汉）生物技术有限责任公司 | A kind of tubular type temperature regulating device and the reaction instrument comprising the tubular type temperature regulating device |
| CN114350760A (en) * | 2022-01-12 | 2022-04-15 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and method for nucleic acid on-site temperature-variable amplification |
| CN114690812A (en) * | 2020-12-31 | 2022-07-01 | 中元汇吉生物技术股份有限公司 | Temperature control unit, temperature control unit and temperature control device |
-
2004
- 2004-04-01 CN CN 200420044241 patent/CN2697102Y/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101287845B (en) * | 2005-05-11 | 2012-07-18 | 先进液体逻辑公司 | Method and device for conducting biochemical or chemical reactions at multiple temperatures |
| CN101363001B (en) * | 2008-08-22 | 2012-06-27 | 金银杏生物科技(北京)有限公司 | Sliding mode heat transfer media plate PCR instrument |
| CN103911283A (en) * | 2014-03-18 | 2014-07-09 | 北京工业大学 | Spiral tri-prism PCR micro-fluidic apparatus |
| CN105602842A (en) * | 2016-01-14 | 2016-05-25 | 江苏猎阵生物科技有限公司 | One-click nucleic acid amplification instrument and application thereof |
| CN109022263A (en) * | 2017-06-08 | 2018-12-18 | 昆泰锐（武汉）生物技术有限责任公司 | A kind of tubular type temperature regulating device and the reaction instrument comprising the tubular type temperature regulating device |
| CN107446811A (en) * | 2017-07-25 | 2017-12-08 | 新疆昆泰锐生物技术有限公司 | A kind of reaction instrument for PCR tubular type temperature regulating device and comprising the device |
| CN114690812A (en) * | 2020-12-31 | 2022-07-01 | 中元汇吉生物技术股份有限公司 | Temperature control unit, temperature control unit and temperature control device |
| CN114350760A (en) * | 2022-01-12 | 2022-04-15 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and method for nucleic acid on-site temperature-variable amplification |
| CN114350760B (en) * | 2022-01-12 | 2024-04-12 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and amplification method for on-site variable-temperature amplification of nucleic acid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050504 Termination date: 20110401 |