CN210065760U

CN210065760U - PCR tube and PCR plate for PCR detection of plant pathogenic bacteria

Info

Publication number: CN210065760U
Application number: CN201920169358.5U
Authority: CN
Inventors: 魏霜; 刘海军; 梁帆; 胡学难
Original assignee: Inspection and Quarantine Technology Center of Guangdong Entry Exit Inspection and Quarantine Bureau
Current assignee: Guangdong Huawei Testing Co ltd; Guangzhou Customs Technology Center
Priority date: 2019-01-30
Filing date: 2019-01-30
Publication date: 2020-02-14
Anticipated expiration: 2029-01-30

Abstract

The utility model discloses a PCR pipe and PCR board for plant pathogenic bacteria PCR detects, the PCR pipe includes bottom closed open-top's body, insert the tube cap of establishing the mouth of pipe department of body, the tube cap stretches into the inside connecting portion of body and is located the outside block portion of body when closing including the lid, the surface that connecting portion are close to block portion is equipped with the external screw thread, the body is close to orificial internal surface and is equipped with the internal thread, the internal surface of body is located internal thread below circumference and is equipped with the soft glue layer, the soft glue layer encircles body a week and forms set tubular structure, when the lid closes the tube cap, connecting portion extend to the soft glue layer. Because the tube cap and the tube body of the PCR tube are sealed by the threaded connection and the soft rubber layer, the double sealing can prevent the tube cap from being separated, the sealing is more stable, the phenomena of expansion, looseness of the tube cap and the like after the amplification reaction are avoided, and the occurrence of false positive is avoided. Meanwhile, the pipe cover can be opened and closed by only screwing the pipe cover with smaller force, and the operation is more labor-saving.

Description

PCR tube and PCR plate for PCR detection of plant pathogenic bacteria

Technical Field

The utility model relates to a PCR instrument technical field, in particular to a PCR pipe and PCR board for plant pathogenic bacteria PCR detects.

Background

The polymerase chain amplification (PCR) technology invented by KaryMullis is a revolutionary breakthrough in the field of molecular biology in the 80 th 20 th century. With the development of scientific technology, various nucleic acid detection techniques play an extremely important role in basic research such as molecular biology, medicine and law, and application fields such as medical diagnosis and food safety detection. At present, the commonly used nucleic acid amplification methods are based on two major categories of classical PCR and emerging isothermal amplification technologies, such as cross-primer isothermal amplification (CPA), loop-mediated isothermal amplification (LAMP), Strand Displacement Amplification (SDA), helicase-dependent amplification (HDA), Recombinant Polymerase Amplification (RPA), recombinase-mediated amplification (RAA), Rolling Circle Amplification (RCA), and rapid isothermal amplification (RIDA). Due to the high sensitivity of nucleic acid amplification techniques, the yield of amplificate is high.

The Polymerase Chain Reaction (PCR) is a common pathogen bacteria molecule detection technology and comprises three basic Reaction steps of denaturation, annealing and extension, wherein ① template DNA is denatured, namely, after the template DNA is heated to about 94 ℃ for a certain time, a double strand of the template DNA or a double strand DNA formed by PCR amplification is dissociated into a single strand so that the single strand of the template DNA or the double strand DNA formed by PCR amplification is combined with a primer to prepare for the next round of Reaction, ② template DNA and primer annealing (renaturation), namely, after the template DNA is heated to be denatured into a single strand, the temperature is reduced to about 55 ℃, the primer and the complementary sequence of the template DNA are paired and combined, ③ primer extension, namely, a DNA template combination can be easily amplified by using dNTP as a raw material under the action of DNA Polymerase, the complementary sequence and the complementary sequence of the template DNA, the primer and the complementary sequence are combined in a pairing and combination mode of the primer and the complementary sequence of the primer and the primer can be easily copied into a new half-strand DNA extension, so that the primer and the primer can be easily obtained by repeated denaturation, the three-half-strand extension and the primer extension can be easily performed under the condition that the repeated target amplification can be controlled by the PCR amplification and the repeated target amplification temperature, and the target amplification process can not easily occur.

The PCR pipe that uses in the appraisal PCR of current plant pathogenic bacterium detects, it is sealed to utilize the tube cap to peg graft inside the mouth of pipe of body during the saving, but current PCR pipe, the body of PCR tube sheet is whole to be made by a hard material, material elasticity can be poor, sealed effect is not good, it is sealed unreliable, though such structural design makes the PCR pipe more convenient when using, nevertheless can appear expanding after the amplification reaction, phenomenons such as tube cap lax cause the aerosol pollution problem in laboratory or scene easily, thereby make the testing result appear the problem such as false positive. This limits the nucleic acid amplification technology to a certain extent and cannot be widely popularized and applied, especially for field or on-site nucleic acid detection. For realizing better sealed effect, it is generally that the block dynamics between reinforcing tube cap and the body makes to combine inseparabler between tube cap and the body, but just so need use great power just can open and closed tube cap, and the operation is harder, uses inconveniently. Therefore, it is urgent to develop a PCR tube that is resistant to swelling, strictly airtight, and easy to open and close.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide a prevent that the tube cap from breaking away from, improving sealed effect and easily opening and the PCR board that the PCR pipe that is used for plant pathogenic bacteria PCR to detect and can stably place the PCR pipe that the lid closed the operation.

In order to solve the technical problem, the utility model discloses a technical scheme does:

the utility model provides a PCR pipe for plant pathogenic bacteria PCR detects, includes bottom closed open-top's body, inserts the tube cap of establishing the mouth of pipe department of body, the tube cap stretches into the inside connecting portion of body and is located the outside block portion of body when closing including the lid, the surface that connecting portion are close to block portion is equipped with the external screw thread, the body is close to orificial internal surface and is equipped with the internal thread, the internal surface of body is located internal thread below circumference and is equipped with the soft glue layer, and the soft glue layer encircles a body a week and forms set of barrel structure, and the integrated into one piece structure is moulded plastics through the extra quality between soft glue layer and the body through the double-unit, and when the lid closed the tube cap, connecting portion extended to on.

Preferably, the inner surface of body is located the internal thread below and has seted up the mounting groove that matches with the flexible glue layer, the flexible glue layer sets up in the mounting groove.

Preferably, the thickness of the soft adhesive layer is 0.1mm-0.2 mm.

Preferably, when the tube cover is closed, the length of the connecting part extending to the soft rubber layer is 1/5-2/5 of the length of the connecting part.

Preferably, the outer surface of the side wall of the cap part is provided with anti-slip grains.

Preferably, the PCR tube is made of PP or COC.

Preferably, the material of the soft rubber layer is TPV, TPE or TPU.

The utility model also discloses a PCR board, including base and mounting hole, a plurality of mounting holes have been seted up to the base, PCR pipe is inserted respectively and is established in corresponding mounting hole, the inside wall of mounting hole is equipped with the recess, the mouth of pipe surface of PCR pipe be equipped with recess complex flange.

Preferably, the groove is circumferentially arranged along the inner side wall of the mounting hole, the flange is circumferentially arranged along the outer side surface of the pipe orifice of the PCR pipe, and the annular flange is tightly clamped in the annular groove.

Preferably, the base is made of PC.

Adopt above-mentioned technical scheme, the utility model discloses a PCR pipe for plant pathogenic bacteria PCR detects, because the tube cap and the body of PCR pipe adopt threaded connection and flexible glue layer to seal, during the use, in the connecting portion screw in body of tube cap, strengthen the tube cap through the screw-thread fit with the seal strength of body, connecting portion extend to on the hose lining, double sealing can prevent that the tube cap from breaking away from, improve sealed effect, it is sealed more reliable, it is more firm, avoid can appear the inflation after the amplification reaction, phenomenons such as tube cap lax, solve aerosol pollution problem, thereby avoid the appearance of false positive. Only need simultaneously to twist and move the tube cap, use less power just can open and closed tube cap, operate more laborsavingly, open with closed operation comfort good, the utility model discloses a PCR board, PCR pipe are connected with the base circumference block that has the mounting hole for PCR pipe combines into an organic whole with the base better, combines more firmly, improves the anti shear strength between PCR pipe and the base, improves life.

Drawings

FIG. 1 is a cross-sectional view of a PCR tube of the present invention;

FIG. 2 is a schematic structural view of a tube cap in the PCR tube of the present invention;

FIG. 3 is a schematic structural view of the tube body in the PCR tube of the present invention;

FIG. 4 is a schematic structural view of the PCR tube of the present invention after installation;

FIG. 5 is a schematic structural diagram of the PCR plate of the present invention.

Detailed Description

The following describes the present invention with reference to the accompanying drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features related to the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

As shown in fig. 1-3, a PCR tube for plant pathogenic bacteria PCR detection, including bottom closed open-top's body 1, insert the tube cap 2 of establishing in the mouth of pipe department of body 1, tube cap 2 stretches into the inside connecting portion 21 of body 1 and is located the outside block portion 22 of body 1 when closing including the lid, the surface that is close to block portion 22 of connecting portion 21 is equipped with external screw thread 211, the internal surface that body 1 is close to mouth of pipe 11 is equipped with internal thread 111, and the internal surface of body 1 is located internal thread 111 below circumference and is equipped with soft glue layer 112, and soft glue layer 112 encircles body 1 a week and forms cover column structure, and the integrated into one piece structure is formed through the two material integrated into one piece mode combination that moulds plastics between soft glue layer 112 and body 1, and when closing tube cap 2, connecting portion 21 extends to on the soft glue layer 112. Through the sealing strength of screw-thread fit reinforcing tube cap 2 and body 1, connecting portion 21 extends to and seals on the soft glue layer 112, and double seal can prevent that tube cap 2 from breaking away from, improves sealed effect, and is sealed more reliable, and is more firm, avoids can appear expanding after the amplification reaction, and phenomena such as tube cap 2 lax solve aerosol pollution problem to avoid false positive appearance. The external screw thread 211 of connecting portion 21 is 3 ~ 6 circles, and the number of screw threads is too little, and the sealing strength of body 1 and tube cap 2 is weaker, and the number of screw threads is too much, and then the lid closes and opens the operation comparatively loaded down with trivial details, the length of external screw thread 211 accounts for 1/2 of connecting portion 21 length, connecting portion 21 extends to length on the hose layer 112 accounts for 1/5-2/5 of connecting portion 21 length.

The internal surface of body 1 is located internal thread 111 below and has seted up mounting groove (not shown in the figure) that matches with flexible glue layer 112, flexible glue layer 112 sets up in the mounting groove, the above-mentioned mode of setting up of flexible glue layer 112 is difficult for wearing and tearing, is difficult for droing and durable. The hose glue layer 112 needs certain elasticity as the sealing layer, the thickness of hose glue layer 112 is 0.1mm-0.2mm, the connecting portion 21 of tube cap 2 partly with body threaded connection, another part of connecting portion 21 of tube cap 2 with the soft glue layer 112 block, in opening and the closed operation, only need twist the block portion 22 of tube cap 2, just can open and close tube cap 2 with less power, the operation is more laborsaving, it is good to open and close the operation comfort, double sealing improves sealed effect simultaneously, it is sealed more reliable, it is more firm, ensure the effectual sealing connection of tube cap 2 and body 1, the phenomenon that the mouth of pipe 11 that its inside liquid is heated vaporization inflation extrudes tube body 1 with tube cap 2 when preventing body 1 from heating causes tube cap 2 to drop, improve the accuracy of experiment.

The connecting part 21 is a hollow cylindrical structure; the outer surface of the side wall of the cap part 22 is provided with anti-slip lines which can prevent slipping and facilitate screwing of the tube cap 2.

The PCR tube is made of PP or COC.

The soft adhesive layer 112 is made of TPV, TPE or TPU. The high-elasticity sealing material has good elasticity, compression deformation resistance, environment resistance, aging resistance, excellent permanent deformation resistance, tensile strength, high toughness and high resilience, and is good in sealing effect. The soft rubber layer 112 made of TPV, TPE or TPU and the pipe body 1 made of PP or COC are combined to form an integrated structure in a double-material injection molding integrated mode, so that the PCR pipe has good comprehensive physical performance and better sealing performance.

As shown in fig. 4 and 5, a PCR plate comprises a base 3 and mounting holes 4, the base 3 is provided with a plurality of mounting holes 4, PCR tubes are respectively inserted into the corresponding mounting holes 4, the inner side walls of the mounting holes 4 are provided with grooves (not shown), and the outer surface of the tube opening of the tube body 1 of each PCR tube is provided with a flange 113 matched with the grooves.

The groove is circumferentially arranged along the inner side wall of the mounting hole 4, the flange is circumferentially arranged along the outer side surface of the pipe orifice of the PCR pipe, and the annular flange is tightly clamped in the annular groove. The base 3 is made of PC. The PCR pipe is arranged in the mounting hole 4 and is connected with the base 3 in a circumferential clamping manner, so that the PCR pipe is combined with the base 3 into a whole better, the combination is firmer, the shearing strength between the PCR pipe and the base 3 is improved, and the service life is prolonged.

The utility model discloses a use as follows: when in use, the PCR tube is firstly placed into the mounting hole 4, and the annular flange of the PCR tube is tightly clamped in the annular groove of the mounting hole 4, so that the PCR tube is well clamped and connected with the mounting hole 4; then, adding the sample into the tube body 1, screwing the connecting part 21 of the tube cover 2 into the tube body 1 after the sample is added, so that the connecting part and the tube cover are in threaded fit, and meanwhile, the connecting part 21 extends onto the soft rubber layer 112, so that double sealing is realized, the sealing effect is improved, the sealing is more reliable and more stable, the tube cover 2 and the tube body 1 are effectively connected in a sealing manner, the tube cover 2 is prevented from being separated, the phenomena of expansion, looseness and the like of the tube cover 2 after the amplification reaction is finished are avoided, the aerosol pollution problem is solved, and the occurrence of false positive is avoided; finally, after the PCR reaction is completed, the PCR tube is taken out, and the tube cap 2 is screwed out and opened.

The utility model discloses a PCR pipe, because tube cap 2 and body 1 adopt threaded connection and flexible glue layer 112 to seal, during the use, in the connecting portion 21 screw in body 1 of tube cap 2, through the seal strength of screw-thread fit reinforcing tube cap 2 with body 1, connecting portion 21 extends to on the flexible glue layer 112, double sealing can prevent that tube cap 2 from breaking away from, improve sealed effect, it is sealed more reliable, more firm, avoid can appearing the inflation after the amplification reaction, phenomenons such as tube cap 2 lax, solve aerosol pollution problem, thereby avoid the appearance of false positive. Meanwhile, only the pipe cover 2 needs to be screwed, the pipe cover 2 can be opened and closed by using smaller force, the operation is more labor-saving, and the comfortable feeling of the opening and closing operation is good.

The utility model discloses a PCR board, PCR pipe pack into mounting hole 4 in, are connected with 3 circumference blocks of base for the PCR pipe combines into an organic whole better with base 3, combines more firmly, improves the shear strength between PCR pipe and the base 3, improves life.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and the scope of the invention is to be accorded the full scope of the claims.

Claims

1. The utility model provides a PCR pipe for plant pathogenic bacteria PCR detects, includes that the bottom seals open-top's body, inserts the tube cap of establishing the mouth of pipe department of body, its characterized in that: the utility model discloses a pipe lid, including the body, the body is close to orificial internal surface and is equipped with the internal thread, the body internal surface is located internal thread below circumference and is equipped with the soft glue layer, and the soft glue layer encircles a body a week and forms cover tubular structure, combines to form the integral type structure through the double-material integrated into one piece mode of moulding plastics between soft glue layer and the body, when the lid closes the body, connecting portion extend to on the soft glue layer.

2. The PCR tube for PCR detection of plant pathogenic bacteria according to claim 1, characterized in that: the inner surface of the pipe body is located below the internal thread and is provided with a mounting groove matched with the soft rubber layer, and the soft rubber layer is arranged in the mounting groove.

3. The PCR tube for PCR detection of plant pathogenic bacteria according to claim 1, characterized in that: the thickness of the soft adhesive layer is 0.1mm-0.2 mm.

4. The PCR tube for PCR detection of plant pathogenic bacteria according to claim 1, characterized in that: when the tube cover is closed, the length of the connecting part extending to the soft rubber layer accounts for 1/5-2/5 of the length of the connecting part.

5. The PCR tube for PCR detection of plant pathogenic bacteria according to claim 1, characterized in that: the outer surface of the side wall of the cap part is provided with anti-skid grains.

6. The PCR tube for PCR detection of plant pathogenic bacteria according to claim 1, characterized in that: the PCR tube is made of PP or COC.

7. The PCR tube for PCR detection of plant pathogenic bacteria according to claim 1, characterized in that: the soft rubber layer is made of TPV, TPE or TPU.

8. A PCR plate comprising the PCR tube according to any one of claims 1 to 7, wherein: the PCR tube inserting device comprises a base and mounting holes, wherein the base is provided with a plurality of mounting holes, the PCR tubes are respectively inserted in the corresponding mounting holes, the inner side walls of the mounting holes are provided with grooves, and the outer surfaces of the tube openings of the PCR tubes are provided with flanges matched with the grooves.

9. The PCR plate of claim 8, wherein: the base is made of PC.