CN210065753U - Stem cell filtering separator - Google Patents

Stem cell filtering separator Download PDF

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Publication number
CN210065753U
CN210065753U CN201920907964.2U CN201920907964U CN210065753U CN 210065753 U CN210065753 U CN 210065753U CN 201920907964 U CN201920907964 U CN 201920907964U CN 210065753 U CN210065753 U CN 210065753U
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CN
China
Prior art keywords
filter membrane
tank body
stem cell
separation tank
lower filter
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Active
Application number
CN201920907964.2U
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Chinese (zh)
Inventor
刘玮
李高伟
邓姗姗
董向涛
吴子谦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hebei Sanzang Biotechnology Co Ltd
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Hebei Sanzang Biotechnology Co Ltd
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Priority to CN201920907964.2U priority Critical patent/CN210065753U/en
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Abstract

The utility model relates to a stem cell filtering separator, which comprises a separating tank body, a collecting tank, an upper filter membrane, a lower filter membrane and an agitating mechanism, wherein the bottom of the separating tank body is fixedly provided with the collecting tank, the bottom opening of the separating tank body is arranged, the inside of the separating tank body is fixedly provided with two layers of filter membranes, each filter membrane comprises the upper filter membrane and the lower filter membrane, the lower filter membrane is arranged close to the collecting tank, the inside of the separating tank body is provided with the agitating mechanism, the top of the separating tank body is provided with a liquid inlet, the separating tank body at one side of the lower filter membrane is provided with a liquid outlet, one side at the bottom of the collecting tank is provided with a waste liquid port, the utility model has simple structure, promotes the flow of liquid in the separating tank body, changes the traditional mechanical stirring mode, avoids the damage of mechanical stirring to cells while promoting the separation of stem, the practicability is stronger.

Description

Stem cell filtering separator
Technical Field
The utility model relates to a stem cell separation technical field especially relates to a stem cell filter separator.
Background
Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent (multipotent). The stem cells are not fully differentiated and immature cells, have the potential function of regenerating various tissues and organs and human bodies, are called as universal cells in the medical field, and when the conventional stem cell filtering and separating device is used, in order to solve the problem of nonuniformity of a filter membrane due to the weight difference of tissue fluid, a motor is adopted to drive a stirring shaft and stirring blades to stir, the mechanical stirring easily causes damage to the cells, the separation quality is influenced, and the practicability is poor.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects in the prior art and providing a stem cell filtering separator.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a stem cell filters separator, includes the knockout drum body, collecting vat, goes up filter membrane, lower filter membrane and rabbling mechanism, the bottom fixed mounting of the knockout drum body has the collecting vat, the bottom opening of the knockout drum body sets up, the inside fixed mounting of the knockout drum body has two-layer filter membrane, and the filter membrane includes last filter membrane and lower filter membrane, the filter membrane is close to the collecting vat setting down, the internal mounted of knockout drum has rabbling mechanism, the inlet has been seted up at the top of the knockout drum body, the liquid outlet has been seted up on the knockout drum body of filter membrane one side down, the waste liquid mouth has been seted up to one side of collecting vat bottom, the equal fixed mounting in edge of going up filter membrane.
Preferably, the stirring mechanism includes air duct, air cock, air pump and return air inlet, air duct fixed mounting is in the knockout drum internal portion, the one end of air duct extends to lower filter membrane, the one end that lower filter membrane was kept away from to the air duct is passed the knockout drum and is given vent to anger the end through connecting pipe and air pump and be connected, the inlet end of air pump is passed through the connecting pipe and is connected with the return air inlet, return air inlet fixed mounting is at the knockout drum top.
Preferably, the upper filter membrane and the lower filter membrane are fixedly installed through a support bracket, the support bracket is arranged in a U-shaped structure, and a rubber pad is installed between the support bracket and the filter membrane plate.
Preferably, the diameter of the filtration pore of the upper filtration membrane is 1.5 times that of the filtration pore of the lower filtration membrane.
Preferably, the return air port is the trapezium structure setting, the great one end of return air port opening sets up down, the inside fixed mounting water-stop film of the great one end of return air port opening.
Preferably, the bottom of the separation tank body is in a conical structure, and threaded sealing covers are detachably mounted on the liquid inlet, the waste liquid port and the liquid outlet.
The utility model provides a pair of stem cell filtering separator, beneficial effect lies in:
1. this stem cell filter separator, simple structure, convenient to use, install the last filter membrane in the internal portion of knockout drum and the filtration separation that lower filter membrane realized stem cell, install the rabbling mechanism in the internal portion of knockout drum, the air pump erupts jar internal portion air by the air cock on the air duct, promotes the flow of the internal liquid of knockout drum, changes the mode that the tradition adopted mechanical stirring, avoids stirring machinery to the damage of cell when promoting stem cell and other cell separation, improves the efficiency and the quality of separation.
2. According to the stem cell filtering separator, the filtering membrane plates arranged on the upper filtering membrane and the lower filtering membrane are matched with the support bracket, and the rubber pads are arranged between the filtering membrane plates and the support bracket, so that the installation stability of the upper filtering membrane and the lower filtering membrane is improved; adopt the internal air circulation of knockout drum to blast liquid, reduce the influence of external environment to the cell, the practicality is stronger.
Drawings
Fig. 1 is a schematic structural view of a stem cell filtration separator according to the present invention;
fig. 2 is a schematic structural view of a stem cell filtration separator a according to the present invention;
fig. 3 is a schematic structural diagram of a stem cell filtration separator B according to the present invention;
fig. 4 is a schematic structural diagram of an upper filter membrane of a stem cell filtration separator according to the present invention.
In the figure: 1. separating the tank body; 2. collecting tank; 3. an upper filter membrane; 4. a lower filter membrane; 5. a stirring mechanism; 51. an air duct; 52. an air tap; 53. a connecting pipe; 54. an air pump; 55. an air return port; 56. a water-barrier film; 6. a liquid inlet; 7. a waste liquid port; 8. a liquid outlet; 9. a membrane filtering plate; 10. a support bracket; 11. and (7) a rubber pad.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1-4, a stem cell filters separator, includes the knockout drum body 1, collecting vat 2, upper filter membrane 3, lower filter membrane 4 and rabbling mechanism 5, the bottom fixed mounting of the knockout drum body 1 has collecting vat 2, the opening sets up in the 1 bottom of the knockout drum body, the inside fixed mounting of the knockout drum body 1 has two-layer filter membrane, and the filter membrane includes upper filter membrane 3 and lower filter membrane 4, lower filter membrane 4 is close to the setting of collecting vat 2, the internally mounted of the knockout drum body 1 has rabbling mechanism 5, inlet 6 has been seted up at the top of the knockout drum body 1, the liquid outlet 8 has been seted up on the knockout drum body 1 of lower filter membrane 4 one side, waste liquid mouth 7 has been seted up to one side of collecting vat 2 bottom, the equal fixed mounting in edge of upper filter membrane 3 and.
The stirring mechanism 5 comprises an air duct 51, an air tap 52, an air pump 54 and an air return port 55, the air duct 51 is fixedly installed inside the separation tank body 1, one end of the air duct 51 extends to the lower filter membrane 4, one end of the air duct 51 far away from the lower filter membrane 4 passes through the separation tank body 1 and is connected with the air pump 54 through a connecting pipe 53, the air inlet end of the air pump 54 is connected with the air return port 55 through a connecting pipe 53, the air return port 55 is fixedly installed at the top of the separation tank body 1, the upper filter membrane 3 and the lower filter membrane 4 are fixedly installed through a support bracket 10, the support bracket 10 is arranged in a U-shaped structure, a rubber pad 11 is installed between the support bracket 10 and the filter membrane plate 9, the filter hole diameter of the upper filter membrane 3 is 1.5 times of that of the filter hole diameter of the lower filter membrane 4, the air return port 55 is arranged in a trapezoidal structure, the larger end of the air return port 55 is arranged, the separation aqueous vapor gets into air pump 54, the bottom of the knockout drum body 1 is the setting of toper structure, all detachable installs thread sealing lid on inlet 6, waste liquid mouth 7 and the liquid outlet 8.
The working principle is as follows: when the device is used, the sealing cover on the liquid inlet 6 is opened to add liquid into the separation tank body 1 from the liquid inlet 6, the sealing cover on the liquid inlet 6 is covered, the air pump 54 is started, air in the separation tank body 1 enters the air guide pipe 51 from the air return port 55 and the connecting pipe 53 under the action of the air pump 54, then the air nozzle 52 on the air guide pipe 51 sprays the air into the liquid in the separation tank body 1, the air nozzle 52 injects air to stir the liquid in the separation tank body 1 to flow, the problem of nonuniformity of the filter membrane due to the weight difference of tissue liquid is solved by the flow of the liquid, the upper filter membrane 3 and the lower filter membrane 4 which are arranged in the separation tank body 1 realize the filtration and separation of stem cells, the stirring mechanism 5 in the separation tank body 1 changes the traditional mechanical stirring mode, the damage of mechanical stirring to the cells is avoided while the separation of the stem cells from other cells is promoted, opening a liquid outlet 8 on the separation tank body 1 to discharge separation liquid, discharging the liquid penetrating through the filter membrane from an opening at the bottom of the separation tank body 1 to a collecting tank 2, finally opening a waste liquid outlet 7 on the collecting tank 2 to discharge the liquid, matching a filter membrane plate 9 arranged on an upper filter membrane 3 and a lower filter membrane 4 with a support bracket 10, arranging a rubber pad 11 between the filter membrane plate 9 and the support bracket 10, and increasing the installation buffer property by using the rubber pad 11 to improve the installation stability of the upper filter membrane 3 and the lower filter membrane 4; adopt the inside air cycle of the knockout drum body 1 to blast liquid, reduce the influence of external environment to the cell, the practicality is stronger.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (6)

1. A stem cell filtering separator is characterized by comprising a separating tank body (1), a collecting tank (2), an upper filter membrane (3), a lower filter membrane (4) and a stirring mechanism (5), a collecting tank (2) is fixedly arranged at the bottom of the separation tank body (1), an opening is arranged at the bottom of the separation tank body (1), two layers of filter membranes are fixedly arranged in the separation tank body (1), the filter membranes comprise an upper filter membrane (3) and a lower filter membrane (4), the lower filter membrane (4) is arranged close to the collecting tank (2), a stirring mechanism (5) is arranged in the separating tank body (1), a liquid inlet (6) is arranged at the top of the separation tank body (1), a liquid outlet (8) is arranged on the separation tank body (1) at one side of the lower filter membrane (4), a waste liquid port (7) is formed in one side of the bottom of the collecting tank (2), and filter membrane plates (9) are fixedly mounted on the edges of the upper filter membrane (3) and the lower filter membrane (4).
2. The stem cell filtering separator as claimed in claim 1, wherein the agitating mechanism (5) comprises a gas-guiding tube (51), an air nozzle (52), an air pump (54) and a gas return opening (55), the gas-guiding tube (51) is fixedly installed inside the separation tank body (1), one end of the gas-guiding tube (51) extends to the lower filter membrane (4), one end of the gas-guiding tube (51) far away from the lower filter membrane (4) penetrates through the separation tank body (1) and is connected with the gas outlet end of the air pump (54) through a connecting tube (53), the gas inlet end of the air pump (54) is connected with the gas return opening (55) through a connecting tube (53), and the gas return opening (55) is fixedly installed at the top of the separation tank body (1).
3. The stem cell filter separator according to claim 1, wherein the upper filter membrane (3) and the lower filter membrane (4) are fixedly mounted by a support bracket (10), the support bracket (10) is arranged in a U-shaped structure, and a rubber gasket (11) is mounted between the support bracket (10) and the filter membrane plate (9).
4. A stem cell filtration separator according to claim 1, wherein the diameter of the filtration pores of said upper filtration membrane (3) is 1.5 times the diameter of the filtration pores of said lower filtration membrane (4).
5. The stem cell filtering separator as claimed in claim 2, wherein the air return port (55) is in a trapezoidal structure, the larger opening end of the air return port (55) is arranged downward, and a water-stop film (56) is fixedly arranged inside the larger opening end of the air return port (55).
6. The stem cell filtering separator as claimed in claim 1, wherein the bottom of the separation tank body (1) is in a conical structure, and threaded sealing covers are detachably mounted on the liquid inlet (6), the waste liquid port (7) and the liquid outlet (8).
CN201920907964.2U 2019-06-17 2019-06-17 Stem cell filtering separator Active CN210065753U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201920907964.2U CN210065753U (en) 2019-06-17 2019-06-17 Stem cell filtering separator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201920907964.2U CN210065753U (en) 2019-06-17 2019-06-17 Stem cell filtering separator

Publications (1)

Publication Number Publication Date
CN210065753U true CN210065753U (en) 2020-02-14

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ID=69427677

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201920907964.2U Active CN210065753U (en) 2019-06-17 2019-06-17 Stem cell filtering separator

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CN (1) CN210065753U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021164509A1 (en) * 2020-02-19 2021-08-26 上海艾众生物科技有限公司 Cell separation apparatus for bioreactor
CN113854281A (en) * 2021-07-23 2021-12-31 青岛市市立医院(青岛市临床医学研究所、青岛市医学影像中心) Placenta stem cell extract constant temperature storage device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021164509A1 (en) * 2020-02-19 2021-08-26 上海艾众生物科技有限公司 Cell separation apparatus for bioreactor
CN113854281A (en) * 2021-07-23 2021-12-31 青岛市市立医院(青岛市临床医学研究所、青岛市医学影像中心) Placenta stem cell extract constant temperature storage device

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GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: A stem cell filter separator

Effective date of registration: 20220808

Granted publication date: 20200214

Pledgee: Baoding Bank Co., Ltd. High-tech Zone Science and Technology Sub-branch

Pledgor: Hebei Sanzang Biotechnology Co.,Ltd.

Registration number: Y2022130000058

PE01 Entry into force of the registration of the contract for pledge of patent right