Placenta stem cell extract constant temperature storage device
Technical Field
The invention relates to a medical storage device, in particular to a constant-temperature storage device for a placenta stem cell extracting solution.
Background
In recent years, research on stem cells is widely concerned by scientists and generations, and stem cells have extremely wide application prospects in the fields of cell repair of life sciences, developmental biology, pharmacology and the like. At present, the international common storage mode of the placental stem cell extracting solution is to store the obtained stem cell extracting solution in a deep low temperature state.
Application number CN201821610642.3 discloses a placenta stem cell extract constant temperature storage device, including base and condenser, the condenser setting is in the inside of main part, the end-to-end connection of first wire has central processing unit. The temperature is convenient to control, and temperature instability is prevented, so that the quality of the placenta stem cell extracting solution is guaranteed, the placenta stem cell extracting solution appliance can be effectively fixed, and the appliance instability in the conveying process is prevented.
Application number CN202021154271.X discloses a placenta stem cell extract constant temperature storage device, and is big to current storage installation degree of difficulty, the lower problem of storage stability, including the storage box, two fixed blocks about fixed mounting had in the storage box, all seted up on two fixed blocks and accomodate the groove, accomodate the inslot and be equipped with the storing thing, improved storage stability, reduced because the damage that accident such as collision caused.
However, the above devices have simple temperature control concept and low temperature control precision, and cannot be perfectly applied to stem cell extract; since the temperature, fluidity and concentration of the stem cell extract all have an influence on the storage life of the stem cell extract, a special device should be provided for storage.
Disclosure of Invention
In order to solve the above problems, a constant temperature storage device for placental stem cell extract is provided, which comprises a storage box, wherein a control module is arranged in the storage box;
the control module is connected with the temperature control module, the circulation module and the light detection module; the storage box comprises an outer layer heat insulation shell, an inner layer heat insulation shell and a storage inner container; wherein the storage liner is arranged in the inner layer heat-insulating shell, the inner layer heat-insulating shell is arranged in the outer layer heat-insulating shell,
the inner layer heat-insulating shell is provided with a sealing cover;
the temperature control module comprises a refrigeration pipeline and a compressor, wherein the refrigeration pipeline is arranged on the inner-layer heat-insulation shell and is in contact with the storage liner; the refrigeration pipeline is connected with the compressor, and the compressor is connected with the control module;
the storage liner comprises a fine filtration membrane and an ultrafiltration membrane, and the aperture of the fine filtration membrane is 0.1-0.5 μm; the aperture of the ultrafiltration membrane is 50-100 nm; the fine filtration membrane is arranged above the ultrafiltration membrane, so that the placenta stem cell extracting solution entering from the upper inlet of the storage liner passes through the fine filtration membrane and then reaches the space between the fine filtration membrane and the ultrafiltration membrane, and then water can pass through the ultrafiltration membrane and flow into the space below the ultrafiltration membrane;
the circulating module comprises a water inlet pipe, a water outlet pipe and an electric control pump; the electric control pump is connected with the water inlet pipe and the water outlet pipe and is connected with the control module; the water inlet pipe is arranged in the space below the ultrafiltration membrane, and the water outlet pipe is arranged in the space above the fine filtration membrane, so that water flowing in from the lower part of the ultrafiltration membrane can be pumped to the upper part of the fine filtration membrane by the electric control pump;
a one-way liquid outlet penetrating through the outer-layer heat-insulating shell, the inner-layer heat-insulating shell and the storage liner is arranged in a space between the fine filtration membrane and the ultrafiltration membrane, so that the stem cell extracting solution can be discharged from the liquid outlet;
the light detection module is arranged in a space between the fine filtration membrane and the ultrafiltration membrane and used for emitting and receiving infrared light with specific wavelength and calculating the concentration of the stem cell extracting solution in the space between the fine filtration membrane and the ultrafiltration membrane according to the infrared light with specific wavelength.
The aperture of the fine filtration membrane is 0.1 μm, and the aperture of the ultrafiltration membrane is 50 nm.
The circuit board of the control module is arranged on the outer-layer heat-insulation shell and is close to the outer surface of the outer-layer heat-insulation shell; the compressor is arranged on the outer-layer heat-insulation shell and is close to the outer surface of the outer-layer heat-insulation shell so as to dissipate heat;
the inner surface of the storage inner container is made of a high-reflection material, light emitted from the light detection module penetrates through the stem cell extracting solution, is reflected by the high-reflection material and then is received by the light detection module, and the concentration of the stem cell extracting solution is calculated according to the intensity of the received light;
the wavelength of the light emitted by the light detection module is the characteristic absorption wavelength of the stem cell extracting solution.
The wavelength of light emitted from the light detection module is 961.4nm, 1247.3nm or 2547.1 nm.
The control module receives the intensity data of the light detected by the light detection module and calculates the intensity data to obtain the concentration of the stem cell extracting solution; and comparing the calculated concentration with a threshold value, when the concentration is higher than the threshold value, controlling the electric control pump to increase the pump pressure by the control module so as to increase the water flow rate in the water outlet pipe, and when the concentration is lower than the threshold value, controlling the electric control pump to decrease the pump pressure by the control module so as to decrease the water flow rate in the water outlet pipe.
The device ensures the relative stability of the concentration of the stem cell extracting solution while ensuring the fluidity of water, and improves the storage time of the stem cell extracting solution.
One side of the inner-layer heat-insulating shell, which is provided with the water inlet pipe, the water outlet pipe and the electric control pump, is not provided with a refrigeration pipeline, so that the water inlet pipe, the water outlet pipe and the electric control pump are prevented from being condensed;
the refrigerating temperature of the refrigerating pipeline is stabilized at 0-5 ℃, and a temperature detector is arranged in the water inlet pipe; the temperature detector sends the detected temperature to the control module, and when the temperature detected by the temperature detector is lower than 0 ℃, the control module controls the compressor to reduce the refrigerating capacity, so that the temperature is increased; when the temperature detected by the temperature detector is higher than 5 ℃, the control module controls the compressor to increase the refrigerating capacity, so that the temperature is reduced, and the relative constancy of the temperature is realized.
The outer surface of the outer-layer heat-insulation shell is made of glass fiber reinforced plastic materials, and when the sealing cover covers the inner-layer heat-insulation shell, the upper surface of the sealing cover and the upper surface of the outer-layer heat-insulation shell are in the same plane; the upper surface of the sealing cover is made of glass fiber reinforced plastic material.
The invention has the beneficial effects that:
because the speed of the placenta stem cell extracting solution passing through the fine filter membrane and the speed of the water passing through the ultrafiltration membrane are slow, and meanwhile, the effective components in the stem cell extracting solution can pass through the fine filter membrane but cannot pass through the ultrafiltration membrane, the fine filter membrane plays a role in filtering impurities, and the ultrafiltration membrane plays a role in extracting the water from the stem cell extracting solution;
when the effect is actually generated, after impurities are filtered by the fine filtration membrane, the stem cell extracting solution enters the lower part of the fine filtration membrane, and then water can pass through the ultrafiltration membrane and reach the lower part of the ultrafiltration membrane, so that the impurities above the fine filtration membrane can be fully washed by circulating the water once, and meanwhile, the water can be ensured to flow and be clean pure water; the flow of water can improve the activity of the stem cell extracting solution, and meanwhile, the concentration of the stem cell extracting solution can be relatively stable in the recycling process, so that the activity of the stem cell extracting solution is further improved.
Simultaneously because the temperature is measured in inlet tube department, measured position is the most accurate, has guaranteed temperature control's accuracy nature, and the active component part of no longer stem cell extract simultaneously sets up thermodetector, can reduce the influence of temperature detection to stem cell extract, also reduces the influence of stem cell extract to thermodetector, improves the life-span of device.
Drawings
The accompanying drawings, which are included to provide a further understanding of the disclosed subject matter, are incorporated in and constitute a part of this specification. The drawings illustrate the implementations of the disclosed subject matter and, together with the detailed description, serve to explain the principles of implementations of the disclosed subject matter. No attempt is made to show structural details of the disclosed subject matter in more detail than is necessary for a fundamental understanding of the disclosed subject matter and various modes of practicing the same.
FIG. 1 is a schematic block diagram of the present invention;
FIG. 2 is a schematic view of the structure of the apparatus of the present invention.
Detailed Description
The advantages, features and methods of accomplishing the same will become apparent from the drawings and the detailed description that follows.
Example (b):
with reference to fig. 1-2, a constant temperature storage device for placental stem cell extract comprises a storage box, wherein a control module is arranged in the storage box;
the control module is connected with the temperature control module, the circulating module and the light detection module 01; the storage box comprises an outer layer heat insulation shell 02, an inner layer heat insulation shell 03 and a storage liner; wherein, the storage liner is arranged in the inner layer heat preservation shell 03, the inner layer heat preservation shell 03 is arranged in the outer layer heat preservation shell 02,
the inner layer heat preservation shell 03 is provided with a sealing cover 04;
the temperature control module comprises a refrigeration pipeline 05 and a compressor 06, wherein the refrigeration pipeline 05 is arranged on the inner-layer heat-insulation shell 03 and is in contact with the storage liner; the refrigeration pipeline 05 is connected with the compressor 06, and the compressor 06 is connected with the control module;
the storage inner container comprises a fine filtration membrane 07 and an ultrafiltration membrane 08, and the aperture of the fine filtration membrane 07 is 0.1-0.5 μm; the aperture of the ultrafiltration membrane 08 is 50-100 nm; the fine filtration membrane 07 is arranged above the ultrafiltration membrane 08, so that the placenta stem cell extracting solution entering from the upper inlet of the storage liner passes through the fine filtration membrane 07 and then reaches the space between the fine filtration membrane 07 and the ultrafiltration membrane 08, and then water can pass through the ultrafiltration membrane 08 and flow into the space below the ultrafiltration membrane 08;
the circulating module comprises a water inlet pipe 09, a water outlet pipe 10 and an electric control pump 11; the electric control pump 11 is connected with the water inlet pipe 09 and the water outlet pipe 10, and the electric control pump 11 is connected with the control module; the water inlet pipe 09 is arranged in the space below the ultrafiltration membrane 08, and the water outlet pipe 10 is arranged in the space above the fine filtration membrane 07, so that water flowing from the lower part of the ultrafiltration membrane 08 can be pumped to the upper part of the fine filtration membrane 07 by the electric control pump 11;
a one-way liquid outlet 12 penetrating through the outer-layer heat-insulating shell 02, the inner-layer heat-insulating shell 03 and the storage liner is arranged in a space between the fine filtration membrane 07 and the ultrafiltration membrane 08, so that the stem cell extracting solution can be discharged from the liquid outlet;
the light detection module 01 is arranged in the space between the fine filtration membrane 07 and the ultrafiltration membrane 08 and is used for emitting and receiving infrared light with specific wavelength and calculating the concentration of the stem cell extracting solution in the space between the fine filtration membrane 07 and the ultrafiltration membrane 08 according to the infrared light with specific wavelength.
The pore diameter of the fine filtration membrane 07 is 0.1 μm, and the pore diameter of the ultrafiltration membrane 08 is 50 nm.
The circuit board 13 of the control module is arranged on the outer-layer heat-insulation shell 02 and is close to the outer surface of the outer-layer heat-insulation shell 02; the compressor 06 is arranged on the outer-layer heat-insulating shell 02 and close to the outer surface of the outer-layer heat-insulating shell 02 so as to dissipate heat;
the inner surface of the storage inner container is made of a high-reflection material, light emitted from the light detection module 01 passes through the stem cell extracting solution, is reflected by the high-reflection material and is received by the light detection module 01, and the concentration of the stem cell extracting solution is calculated according to the intensity of the received light;
the wavelength of the light emitted by the light detection module 01 is the characteristic absorption wavelength of the stem cell extract.
The wavelength of light emitted from the photodetector module 01 is 961.4nm, 1247.3nm, or 2547.1 nm.
The control module receives the intensity data of the light detected by the light detection module 01 and calculates the intensity data to obtain the concentration of the stem cell extracting solution; and comparing the calculated concentration with a threshold value, when the concentration is higher than the threshold value, the control module controls the electric control pump 11 to increase the pump pressure so as to increase the water flow rate in the water outlet pipe 10, and when the concentration is lower than the threshold value, the control module controls the electric control pump 11 to decrease the pump pressure so as to decrease the water flow rate in the water outlet pipe 10.
The refrigeration pipeline 05 is not arranged on one side of the inner-layer heat-insulation shell 03, which is provided with the water inlet pipe 09, the water outlet pipe 10 and the electric control pump 11, so that the water inlet pipe 09, the water outlet pipe 10 and the electric control pump 11 are prevented from being condensed;
the refrigerating temperature of the refrigerating pipeline 05 is stabilized at 0-5 ℃, and a temperature detector is arranged in the water inlet pipe 09; the temperature detector sends the detected temperature to the control module, and when the temperature detected by the temperature detector is lower than 0 ℃, the control module controls the compressor 06 to reduce the refrigerating capacity, so that the temperature is increased; when the temperature detected by the temperature detector is higher than 5 ℃, the control module controls the compressor 06 to increase the cooling capacity, so that the temperature is lowered, thereby achieving a relatively constant temperature.
The outer surface of the outer-layer heat-insulating shell 02 is made of glass fiber reinforced plastic materials, and when the sealing cover 04 covers the inner-layer heat-insulating shell 03, the upper surface of the sealing cover 04 and the upper surface of the outer-layer heat-insulating shell 02 are in the same plane; the upper surface of the sealing cover 04 is made of glass fiber reinforced plastic material.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.