CN210022520U - Multifunctional centrifuge rotor - Google Patents

Multifunctional centrifuge rotor Download PDF

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Publication number
CN210022520U
CN210022520U CN201920390537.1U CN201920390537U CN210022520U CN 210022520 U CN210022520 U CN 210022520U CN 201920390537 U CN201920390537 U CN 201920390537U CN 210022520 U CN210022520 U CN 210022520U
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China
Prior art keywords
centrifuge
rotor
centrifuge tube
clamping groove
tube clamping
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CN201920390537.1U
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刘珺
陈启跃
陈治远
张彬彬
赵宇鑫
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Beijing jinnuomi Technology Co.,Ltd.
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BEIJING JINNUOMEI BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The application provides a multi-functional centrifuge rotor, centrifuge rotor is made by ABS resin centrifuge rotor is last to be provided with the centrifuging tube draw-in groove of multiple specification simultaneously to can be with the centrifuging tube of the multiple different specifications of centrifugation simultaneously in-process, thereby improve centrifuge's availability factor, improve scientific research personnel's work efficiency, in addition, in different centrifugation processes, if centrifuge tube to different specifications is centrifugated and also need not change centrifuge rotor, thereby further improve scientific research personnel's work efficiency.

Description

Multifunctional centrifuge rotor
Technical Field
The application belongs to biological experimental apparatus, in particular to a multifunctional centrifuge rotor.
Background
The centrifugal machine is an instrument for separating a plurality of components in a composition sample by a centrifugal method, and the analytical separation of the components is realized by utilizing the difference of the sedimentation speeds of different components in a centrifugal force field. The centrifugal machine at least comprises a palm centrifugal machine and a large-scale centrifugal machine according to the specification, wherein the palm centrifugal machine is widely applied to the fields of biomedicine, petrochemical industry, agriculture, food sanitation and the like. Generally, the maximum rotation speed of the palm centrifuge is about 10000rpm, the separation mode is solid-liquid sedimentation separation, and the rotation speed cannot be strictly controlled, and the palm centrifuge is usually operated at room temperature without a refrigeration system, so the palm centrifuge is generally used for collecting easily sedimented large-particle substances, such as red blood cells, yeast cells and the like. Generally, the centrifuge is driven by a multi-purpose alternating current commutator motor, carbon brushes of the motor are easy to wear, and a rotor is arranged on a hard steel shaft, so that the centrifuge tube and contents are very important to be accurately balanced, otherwise, the centrifuge can be damaged.
Centrifuge rotors are important accessories of centrifuges and are important indexes for determining the performance of centrifuges. To the sample of different specifications, need select the centrifuging tube of different specifications for use and hold, its appearance of the centrifuging tube of different specifications is totally different.
At present, the last draw-in groove that only is provided with single shape and aperture of centrifuge rotor that uses usually, promptly, centrifugation at every turn can only centrifuge single specification centrifuging tube, at the intensive scientific research laboratory of experiment, can lead to scientific research work efficiency low, even in time lead to the sample to become invalid owing to failing to centrifugate.
SUMMERY OF THE UTILITY MODEL
The present application provides a centrifuge rotor for a palm centrifuge, in particular for use in real-time fluorescence quantitative analysis.
Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) is a method for measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using fluorescent chemical substances in DNA amplification reaction. In the PCR amplification process, the method carries out quantitative analysis on a specific DNA sequence in a sample to be detected by an internal reference or external reference method according to a fluorescent signal, thereby carrying out real-time detection on the PCR process. The theoretical basis for quantification is that in the exponential phase of PCR amplification, the Ct value of the template and the initial copy number of the template have a linear relationship.
The real-time fluorescence quantitative PCR technology is widely applied to the fields of medical treatment, scientific research, industrial application and the like, such as clinical disease diagnosis, animal disease detection, food safety, quantitative research of medical science, farming and animal husbandry, biological related molecular biology and the like. The real-time fluorescent quantitative PCR technology effectively breaks through the limitation that the traditional quantification only can be used for end-point detection, realizes that the intensity of a fluorescent signal can be detected once in each cycle, records the intensity in computer software, and obtains a quantitative result according to a standard curve by calculating the Ct value of each sample. The quantitative method of the external standard curve is an accurate and reliable scientific method compared with the internal standard method. The real-time fluorescence quantitative PCR using the external standard curve is the quantitative method with the most accurate quantification and the best reproducibility so far, is generally accepted all over the world, and is widely applied to the fields of gene expression research, transgenic research, drug efficacy assessment, pathogen detection and the like.
In real-time fluorescent quantitative PCR, the centrifugal separation of a sample is a very important link, and the accuracy of a detection result is directly influenced by the quality of the centrifugal separation. In addition, PCR samples are generally sensitive to temperature and time, and need to be centrifuged in time after the sample is prepared and transferred to a centrifuge tube.
Fig. 1 is traditional palm centrifuge rotor, as shown in fig. 1, the centrifuge rotor that uses usually only is provided with single centrifuging tube draw-in groove, and not only the function is few, only can place the centrifuging tube of single model, moreover, heavy when this kind of centrifuge rotor, is difficult for being driven by the pivot, leads to centrifugal efficiency to hang down.
The application provides a centrifuge rotor is provided with the centrifuging tube draw-in groove of multiple specification simultaneously to can be with the centrifuging tube of the multiple different specifications of centrifugation in-process while, thereby improve centrifuge's availability factor, improve scientific research personnel's work efficiency, moreover, in different centrifugation processes, if centrifuge the centrifuging tube to different specifications is centrifugated and also need not change centrifuge rotor, thereby further improve scientific research personnel's work efficiency.
The centrifuge rotor comprises a rotor body 1, wherein the rotor body 1 is basin-shaped and comprises a base plate 11 and a side wall 12 which are connected; a first centrifuge tube clamping groove 2, a second centrifuge tube clamping groove 3 and a third centrifuge tube clamping groove 4 are formed in the side wall 12 of the rotor main body 1, wherein the first centrifuge tube clamping groove 2, the second centrifuge tube clamping groove 3 and the third centrifuge tube clamping groove 4 are periodically and uniformly distributed on the rotor main body 1, the cross sections of the first centrifuge tube clamping groove 2 and the second centrifuge tube clamping groove 3 are both circular, the cross section of the third centrifuge tube clamping groove 4 is waist-shaped, the inner diameters of the first centrifuge tube clamping groove 2 and the second centrifuge tube clamping groove 3 are sequentially reduced, and each third centrifuge tube clamping groove 4 is overlapped with one second centrifuge tube clamping groove 3; all the first centrifuge tube clamping grooves 2 are uniformly distributed on the same circumference, all the second centrifuge tube clamping grooves 3 are uniformly distributed on the same circumference, and all the third centrifuge tube clamping grooves 4 are uniformly distributed on the same circumference; a locking piece 5 used for fixing the rotor body 1 on a rotating shaft of a centrifuge is arranged on a chassis 11 of the rotor body.
The utility model provides a set up the centrifuging tube draw-in groove of multiple different specifications and shape simultaneously on the centrifuge rotor, make same rotor can satisfy the centrifugation demand of different model centrifuging tubes, avoid frequently changing centrifuge rotor, operating time has been practiced thrift on the one hand, on the other hand reduces because frequently change the physical damage that causes centrifuge rotor, thereby improve centrifuge rotor's life, and, this centrifuge rotor can be simultaneously to the sample of loading in different model centrifuging tubes centrifugation, thereby improve centrifuge's availability factor and staff's work efficiency.
In an implementable manner, the second centrifuge tube lock slot 3 is concentric in cross section to the third centrifuge tube lock slot 4. The arrangement mode can be used for arranging more types of clamping grooves in the limited space of the side wall of the rotor body, and is convenient for balancing weights of centrifugal tubes.
In an implementation, the center of the first centrifuge tube slot 2 is lower or higher than the center of the second centrifuge tube slot 3. Thus, in the centrifugation process, the centrifuge tube with larger specification is arranged at a lower position, and the centrifuge tube with smaller specification is arranged at a higher position.
In an implementation manner, the first centrifuge tube slot 2, the second centrifuge tube slot 3, and the third centrifuge tube slot 4 are all symmetrically distributed on the side wall 12, so as to facilitate the weight balancing of the centrifuge tubes.
In an implementable manner, the centre line of the waist of the cross section of the third centrifuge tube slot 4 coincides with or is parallel to a generatrix of the side wall 12.
In an implementable manner, the rotor body 1 is made of ABS resin. In the present application, the ABS resin means an acrylonitrile-butadiene-styrene copolymer. The rotor main body prepared from the material is light in weight and high in strength, and can not only improve the rotating speed of the rotor, but also ensure the stability of the rotor structure.
In one implementation, the center line L1 of the first centrifuge tube card slot 2 passes through the center line L of the rotor body, the center line L2 of the second centrifuge tube card slot 3 passes through the center line L of the rotor body, and the center line L3 of the third centrifuge tube card slot 4 passes through the center line L of the rotor body. The centrifuge rotor can still keep balance when rotating at high speed.
In an achievable manner, the angle between the side wall 12 and the base plate 11 is 100 to 150 degrees, preferably 120 degrees.
Compared with the prior art, the application provides a centrifuge rotor can centrifuge the sample that multiple specification centrifuging tube loaded simultaneously, improves centrifuge rotor's utilization ratio and availability factor, moreover, uses ABS resin to make and forms, centrifuge rotor's light in weight, intensity is high, reinforcing centrifugation effect.
Drawings
FIG. 1 shows a schematic structural view of a conventional centrifuge rotor;
FIG. 2 shows a schematic structural diagram of a centrifuge rotor provided by an embodiment of the present application.
Description of the reference numerals
1-rotor body, 11-bottom plate, 12-side wall, 2-first centrifuge tube slot, 3-second centrifuge tube slot, 4-third centrifuge tube slot, and 5-locking piece.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the invention, as detailed in the appended claims.
The structure and operation of a centrifuge rotor according to the present application will be described in detail with reference to the following embodiments.
Fig. 2 is a schematic view illustrating the structure and operation of a centrifuge rotor according to a preferred embodiment. As shown in fig. 2, the centrifuge rotor provided in this embodiment includes a rotor body 1, and the rotor body 1 is basin-shaped and includes a bottom plate 11 and a side wall 12 connected to each other.
In this embodiment, as shown in fig. 2, a first centrifuge tube slot 2, a second centrifuge tube slot 3 and a third centrifuge tube slot 4 are formed on the side wall 12 of the rotor body 1.
Wherein, first centrifuging tube draw-in groove 2, second centrifuging tube draw-in groove 3 and third centrifuging tube draw-in groove 4 are in periodic evenly distributed on the rotor main part 1, first centrifuging tube draw-in groove 2 with the cross section of second centrifuging tube draw-in groove 3 is circular, the cross section of third centrifuging tube draw-in groove 4 is waist shape, first centrifuging tube draw-in groove 2 reduces in proper order with the internal diameter of second centrifuging tube draw-in groove 3, every third centrifuging tube draw-in groove 4 and 3 overlap of a second centrifuging tube draw-in groove.
The centrifuge rotor that this embodiment provided is applied to palm centrifuge, palm centrifuge is multi-purpose in the laboratory, and the centrifuging tube that the laboratory is commonly used includes large centrifuge tube, medium centrifuge tube, small centrifuge tube and four kinds of specifications of microcentrifuge tube at least, and the centrifuging tube of these four kinds of specifications all has the shape of standard dimension and standard, and wherein, the location position of large centrifuge tube to small centrifuge tube is cylindrical, and the difference only lies in the external diameter size at location position, however microcentrifuge tube location position including cylindrical and set up in the location wing of cylindrical both sides, moreover, the external diameter of cylinder is less than and is the cylindrical external diameter of centrifuging tube for a short time in the microcentrifuge tube.
In this embodiment, the internal diameter of first centrifuging tube draw-in groove 2 slightly is greater than the external diameter at large-size centrifuging tube location position, the internal diameter of second centrifuging tube draw-in groove 3 slightly is greater than the external diameter at medium-size centrifuging tube location position, the thickness of third centrifuging tube draw-in groove 4 slightly is greater than the thickness of microcentrifuge tube middle positioning wing.
In this embodiment, can utilize the cylindrical structure of second centrifuging tube draw-in groove 3 to hold the cylindrical position of micro-centrifuging tube to it is right to utilize third centrifuging tube draw-in groove 4 the locating wing is fixed, thereby realizes that centrifugal process is fixed to the micro-quantifying pipe.
In this embodiment, all first centrifuging tube draw-in grooves 2 evenly distributed are on same circumference, and all second centrifuging tube draw-in grooves 3 evenly distributed are on same circumference, and all third centrifuging tube draw-in grooves 4 evenly distributed are on same circumference.
Because first centrifuging tube draw-in groove 2 is used for clamping the centrifuging tube of same kind of specification, consequently, centrifuging tube counter weight when first centrifuging tube draw-in groove 2 evenly distributed is convenient for centrifuge on same circumference to guarantee that centrifuge operation in-process rotor keeps balanced. For the same reason, the clamping grooves of other centrifuge tubes are also uniformly distributed on the same circumference.
In this embodiment, first centrifuging tube draw-in groove 2, second centrifuging tube draw-in groove 3 and third centrifuging tube draw-in groove 4 can distribute in same circumference, also can distribute in different circumferences. Preferably, the clamping grooves are distributed on different circumferences, so that clamping grooves with various specifications are arranged on the side wall 12 in a three-dimensional manner, the use efficiency of the side wall 12 is improved, and the structural strength of the centrifuge rotor is ensured to the maximum extent under the condition that the same number of clamping grooves are arranged.
Further, the applicant finds that the external diameter of the cylindrical part of the microcentrifuge tube is close to the external diameter of the cylindrical part of the medium centrifugal tube, and the distance between the outer edges of the two positioning wings of the microcentrifuge tube is greater than the external diameter of the medium centrifugal tube, so that the applicant superposes the second centrifugal tube clamping groove 3 and the third centrifugal tube clamping groove 4, and the second centrifugal tube clamping groove 3 is in a waist-shaped structure with the third centrifugal tube clamping groove 4 left on the two sides, so that the third centrifugal tube clamping groove 4 is used for fixing the microcentrifuge tube.
In this embodiment, the cross section of the second centrifuge tube slot 3 is concentric with the cross section of the third centrifuge tube slot 4.
The applicant finds that the cross section of the second centrifuge tube clamping groove 3 and the cross section of the third centrifuge tube clamping groove 4 can be concentric to enable the third centrifuge tube clamping groove 4 to be in the lengths extending out of the two sides of the second centrifuge tube clamping groove 3 are equal, so that the stability of a micro-centrifuge tube in a rotor in the centrifugal process can be increased, and the cylindrical space of the second centrifuge tube clamping groove can be fully utilized.
In addition, the arrangement mode can be used for forming clamping grooves with more specifications in the limited space of the side wall of the rotor body, and is convenient for balancing weights of centrifugal tubes.
In this embodiment, the center of the first centrifuge tube slot 2 is lower or higher than the center of the second centrifuge tube slot 3. The applicant has found that this arrangement enables larger format tubes to be located at a lower position and smaller format tubes to be located at a higher position during centrifugation. Generally speaking, the sample size of loading in the great centrifuging tube of specification is great, moreover, is generally used for primary separation, and each component sedimentation rate's difference is great, consequently, can accomplish the separation under the effect of less centrifugal force, and the sample size of loading in the less centrifuging tube of specification is more, moreover, generally is used for accurate separation, and each component sedimentation rate difference is less, consequently, need just can realize the separation under the effect of great centrifugal force. At the same speed, the larger the radius of rotation, the greater the centrifugal force to which the point is subjected. Therefore, the setting mode of this embodiment, the sample that uses different specification centrifuging tubes to bear in same centrifugation operation can both obtain comparatively ideal separation result under the condition of the same rotational speed.
In this embodiment, first centrifuging tube draw-in groove 2, second centrifuging tube draw-in groove 3 and third centrifuging tube draw-in groove 4 are in equal symmetric distribution on lateral wall 12 to centrifuging tube counter weight keeps the rotor balanced in centrifugal process.
In an alternative manner of this embodiment, the center line of the waist shape of the cross section of the third centrifuge tube slot 4 coincides with or is parallel to a generatrix of the side wall 12. The arrangement mode is convenient for taking and placing the micro-centrifugal tube.
In an alternative manner of this embodiment, the center line L1 of the first centrifuge tube card slot 2 passes through the center line L of the rotor body, the center line L2 of the second centrifuge tube card slot 3 passes through the center line L of the rotor body, and the center line L3 of the third centrifuge tube card slot 4 passes through the center line L of the rotor body. The centrifuge rotor can still keep balance when rotating at high speed, and the layer interface of the multi-layer components obtained by centrifugation is vertical or approximately vertical to the axis of the centrifuge tube, thereby facilitating the subsequent treatment after centrifugation.
In a practical manner, the angle between the side wall 12 and the bottom plate 11 is 100 to 150 degrees, preferably 120 degrees, so as to be compatible with most of the sample cups/reaction tubes on the market, and ensure sufficient mechanical and structural strength and product aesthetics.
In this embodiment, a locking member 5 for fixing the rotor body 1 to a centrifuge rotating shaft is provided on a base plate 11 of the rotor body, so that the centrifuge rotor is fixed to the centrifuge rotating shaft, and the centrifuge rotor can rotate at a high speed with the rotation of the rotating shaft.
In the present embodiment, the rotor body 1 is made of ABS resin. In the present application, the ABS resin means an acrylonitrile-butadiene-styrene copolymer. The rotor main body prepared from the material is light in weight and high in strength, and can not only improve the rotating speed of the rotor, but also ensure the stability of the rotor structure.
The utility model provides a set up the centrifuging tube draw-in groove of multiple different specifications and shape simultaneously on the centrifuge rotor, make same rotor can satisfy the centrifugation demand of different model centrifuging tubes, avoid frequently changing centrifuge rotor, operating time has been practiced thrift on the one hand, on the other hand reduces because frequently change the physical damage that causes centrifuge rotor, thereby improve centrifuge rotor's life, and, this centrifuge rotor can be simultaneously to the sample of loading in different model centrifuging tubes centrifugation, thereby improve centrifuge's availability factor and staff's work efficiency.
The present application has been described in detail with reference to specific embodiments and illustrative examples, but the description is not intended to limit the application. Those skilled in the art will appreciate that various equivalent substitutions, modifications or improvements may be made to the presently disclosed embodiments and implementations thereof without departing from the spirit and scope of the present disclosure, and these fall within the scope of the present disclosure. The protection scope of this application is subject to the appended claims.

Claims (9)

1. A multi-functional centrifuge rotor, characterized in that the rotor comprises a rotor body (1), the rotor body (1) being basin-shaped comprising a chassis (11) and a side wall (12) connected;
a first centrifuge tube clamping groove (2), a second centrifuge tube clamping groove (3) and a third centrifuge tube clamping groove (4) are arranged on the side wall (12) of the rotor main body (1), wherein,
the first centrifuge tube clamping groove (2), the second centrifuge tube clamping groove (3) and the third centrifuge tube clamping groove (4) are periodically and uniformly distributed on the rotor main body (1),
the cross sections of the first centrifuge tube clamping groove (2) and the second centrifuge tube clamping groove (3) are both circular, the cross section of the third centrifuge tube clamping groove (4) is waist-shaped,
the inner diameters of the first centrifugal tube clamping groove (2) and the second centrifugal tube clamping groove (3) are sequentially reduced,
each third centrifuge tube clamping groove (4) is overlapped with one second centrifuge tube clamping groove (3);
all the first centrifuge tube clamping grooves (2) are uniformly distributed on the same circumference, all the second centrifuge tube clamping grooves (3) are uniformly distributed on the same circumference, and all the third centrifuge tube clamping grooves (4) are uniformly distributed on the same circumference;
a locking piece (5) used for fixing the rotor body (1) on a rotating shaft of the centrifuge is arranged on a chassis (11) of the rotor body.
2. The centrifuge rotor according to claim 1, characterized in that the cross section of the second centrifuge tube slot (3) is concentric to the cross section of the third centrifuge tube slot (4).
3. The centrifuge rotor according to claim 1, characterized in that the center of the first centrifuge tube slot (2) is lower or higher than the center of the second centrifuge tube slot (3).
4. The centrifuge rotor of claim 1 wherein,
first centrifuging tube draw-in groove (2), second centrifuging tube draw-in groove (3) and third centrifuging tube draw-in groove (4) are in equal symmetric distribution on lateral wall (12).
5. The centrifuge rotor according to claim 1, wherein the centre line of the waist-shaped cross-section of the third centrifuge tube slot (4) coincides with or is parallel to one generatrix of the side wall (12).
6. The centrifuge rotor according to claim 1, characterized in that the rotor body (1) is made of ABS resin.
7. The centrifuge rotor of claim 1 wherein the centerline L1 of the first centrifuge tube card slot (2) passes through the rotor body centerline L, the centerline L2 of the second centrifuge tube card slot (3) passes through the rotor body centerline L, and the centerline L3 of the third centrifuge tube card slot (4) passes through the rotor body centerline L.
8. The centrifuge rotor according to claim 1 characterized in that the angle between the side wall (12) and the bottom disk (11) is 100 to 150 degrees.
9. A centrifuge rotor according to claim 1, characterised in that the angle between the side wall (12) and the chassis (11) is 120 degrees.
CN201920390537.1U 2019-03-26 2019-03-26 Multifunctional centrifuge rotor Active CN210022520U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109772602A (en) * 2019-03-26 2019-05-21 北京金诺美生物技术有限公司 A kind of multifunctional centrifugal machine rotor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109772602A (en) * 2019-03-26 2019-05-21 北京金诺美生物技术有限公司 A kind of multifunctional centrifugal machine rotor

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Address after: 100176 floor 01-5, building 16, yard 25, Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing

Patentee after: Beijing jinnuomi Technology Co.,Ltd.

Address before: 100176 room 407, building 2, No. 19, Yongchang South Road, Yizhuang Economic Development Zone, Daxing District, Beijing

Patentee before: BEIJING GENOME BIOTECHNOLOGY CO.,LTD.