CN102564917A - Novel method for detecting platelet aggregation ability - Google Patents
Novel method for detecting platelet aggregation ability Download PDFInfo
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- CN102564917A CN102564917A CN2010105858241A CN201010585824A CN102564917A CN 102564917 A CN102564917 A CN 102564917A CN 2010105858241 A CN2010105858241 A CN 2010105858241A CN 201010585824 A CN201010585824 A CN 201010585824A CN 102564917 A CN102564917 A CN 102564917A
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Abstract
The invention relates to a novel method for detecting platelet aggregation ability, belonging to the field of clinical laboratory medicine. The method uses an impedance technology detecting instrument which can simultaneously count platelets and measure the particle volume (size) of the platelets to directly detect the platelet aggregation ability of whole blood samples, and plasma is not separated. During detection, one blood sample is divided into two parts, one part of blood sample is directly detected without adding a platelet aggregation inducer, the other part of blood sample is detected after adding the platelet aggregation inducer to carry out induction treatment, and finally the two obtained detecting results as basic data are compared to calculate the platelet aggregation ability.
Description
One, technical field
The present invention relates to a kind of detection method of new platelet aggregation ability, belong to the clinical laboratory medicine field.
Two, background technology
Platelet aggregation ability (or claiming PAR) is a hematoblastic critical function.Conventional detection mainly is to add the aggregation inducing agent and make platelet aggregation isolated be rich in the hematoblastic blood plasma, causes behind the platelet aggregation that the blood plasma turbidity changes, and detects the variation of turbidity and obtains the result than purifying method by photoelectricity.Also have detection methods such as whole blood electric-resistivity method, microscopic counting in addition, but these methods and results poor accuracy, detecting operation are loaded down with trivial details, are not widely used in Clinical detection.
Three, summary of the invention
A kind of detection method of new platelet aggregation ability, utilization can be counted and measure the electrical impedance method detecting instrument of particle volume size simultaneously and carry out to blood platelet, directly adopt whole blood as sample, need not separated plasma.During detection same blood sample is divided into two parts; A blood sample does not add the agent of blood platelet aggregation inducing; Another part blood sample adds the gathering derivant and induces processing, (through luring the blood sample that gathers after agent is handled, maybe be because platelet aggregation generates the bigger platelet aggregation thing of volume; When detecting, be shown as the blood platelet of " big volume " on the instrument or exceed other materials of volume of platelets scope, so its testing result is with to gather the blood sample that agent handles variant without luring.) utilize foregoing electrical impedance method detector to detect two parts of blood samples respectively then, two parts of blood examination information that will obtain at last compare and calculate hematoblastic ability of aggregation in this blood sample.
Adopt the inventive method to detect the platelet aggregation ability, avoided loaded down with trivial details centrifugal preparation process, speed is fast, easy to detect, influence factor is few, and therefore, testing result is more reliable.
Four, embodiment
Embodiment one:
During detection same blood sample is divided into two parts; A blood sample does not add the agent of blood platelet aggregation inducing; And another part blood sample adds and assembles derivant and induce processing, utilizes the electrical impedance method detector to detect two parts of platelet counts and volumes in the blood sample respectively then, and the platelet counts and the volume (size) that will detect two parts of blood samples of acquisition at last compare; Can record blood platelet and lure the hematoblastic gathering situation under the agent effect of gathering, thereby calculate hematoblastic ability of aggregation in this blood sample.
Embodiment two:
During detection same blood sample is divided into two parts; A blood sample does not add the agent of blood platelet aggregation inducing; And another part blood sample adds and assembles derivant and induce processing; Utilize the electrical impedance method detector to detect two parts of blood samples respectively then, mean platelet volume (MPV), blood platelet specific volume capacity (PCT) or the volume of platelets dispersion of distribution parameters such as (PDW) that will detect two parts of blood samples that obtain at last compare, thereby calculate hematoblastic ability of aggregation in this blood sample.
Claims (4)
1. the detection method of a new platelet aggregation ability is characterized in that it is sample that this detection method directly adopts whole blood, need not separated plasma, during detection same blood sample is divided into two parts, and portion directly detects, the agent of a process aggregation inducing detects after handling again.It is big or small with particle volume that this method utilizes the electrical impedance method detecting instrument to detect platelet count, is that the basis compares with the testing result (comprising information such as platelet counts, volume size) of gained, and calculates hematoblastic ability of aggregation.
2. the detection method of a kind of new platelet aggregation ability according to claim 1; The testing result that it is characterized in that this method is used for parameters calculated and not only can comprises platelet counts, volume (size), can also comprise mean platelet volume (MPV), blood platelet specific volume capacity (Pct) and volume of platelets dispersion of distribution parameters such as (PDW).
3. the detection method of a kind of new platelet aggregation ability according to claim 1; It is characterized in that detecting instrument that this detection method is used must provide the information of platelet counts and volume (size) simultaneously; Be the basis with these two basic parameters or other parameters of going out according to these two calculation of parameter, calculate hematoblastic ability of aggregation.
4. the detection method of a kind of new platelet aggregation ability according to claim 1; The blood sample that it is characterized in that utilizing this detection method gathers after agent handles through luring; Maybe be because platelet aggregation generates the bigger platelet aggregation thing of volume; Be shown as the blood platelet of " big volume " during detection on the instrument or exceed other materials of volume of platelets scope, so its testing result is with to gather the blood sample that agent handles variant without luring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105858241A CN102564917A (en) | 2010-12-14 | 2010-12-14 | Novel method for detecting platelet aggregation ability |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105858241A CN102564917A (en) | 2010-12-14 | 2010-12-14 | Novel method for detecting platelet aggregation ability |
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| CN102564917A true CN102564917A (en) | 2012-07-11 |
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| CN2010105858241A Pending CN102564917A (en) | 2010-12-14 | 2010-12-14 | Novel method for detecting platelet aggregation ability |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106959264A (en) * | 2017-03-16 | 2017-07-18 | 江苏柯伦迪医疗技术有限公司 | A kind of continuous counter method platelet function assay system and method |
| CN108918797A (en) * | 2018-05-23 | 2018-11-30 | 武汉友芝友医疗科技股份有限公司 | The reagent method and reagent chip of a kind of blood platelet method for separating and concentrating, Platelet drug |
| CN115792198A (en) * | 2022-12-01 | 2023-03-14 | 南京市第一医院 | Method and system for assisting blood cell analyzer in judging abnormal platelet aggregation |
Citations (3)
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| US5185376A (en) * | 1991-09-24 | 1993-02-09 | The United States Of America As Represented By The Department Of Health And Human Services | Therapeutic inhibition of platelet aggregation by nucleophile-nitric oxide complexes and derivatives thereof |
| US5817519A (en) * | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| CN2890917Y (en) * | 2006-03-08 | 2007-04-18 | 吉林省紫宸光电技术有限责任公司 | Blood cell analyzer sensing device |
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2010
- 2010-12-14 CN CN2010105858241A patent/CN102564917A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5185376A (en) * | 1991-09-24 | 1993-02-09 | The United States Of America As Represented By The Department Of Health And Human Services | Therapeutic inhibition of platelet aggregation by nucleophile-nitric oxide complexes and derivatives thereof |
| US5817519A (en) * | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| CN2890917Y (en) * | 2006-03-08 | 2007-04-18 | 吉林省紫宸光电技术有限责任公司 | Blood cell analyzer sensing device |
Non-Patent Citations (2)
| Title |
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| 丛玉隆等: "流式细胞术测定血小板聚集探讨", 《临床检验杂志》 * |
| 陈松鹤等: "电阻法测定血小板减少患者全血中血小板聚集功能", 《上海医科大学学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106959264A (en) * | 2017-03-16 | 2017-07-18 | 江苏柯伦迪医疗技术有限公司 | A kind of continuous counter method platelet function assay system and method |
| CN108918797A (en) * | 2018-05-23 | 2018-11-30 | 武汉友芝友医疗科技股份有限公司 | The reagent method and reagent chip of a kind of blood platelet method for separating and concentrating, Platelet drug |
| CN108918797B (en) * | 2018-05-23 | 2021-01-19 | 武汉友芝友医疗科技股份有限公司 | Platelet separation and enrichment method, platelet function medicine testing method and reagent chip |
| CN115792198A (en) * | 2022-12-01 | 2023-03-14 | 南京市第一医院 | Method and system for assisting blood cell analyzer in judging abnormal platelet aggregation |
| CN115792198B (en) * | 2022-12-01 | 2024-03-15 | 南京市第一医院 | Method and system for assisting blood cell analyzer in judging abnormal platelet aggregation |
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Application publication date: 20120711 |