CN210803049U - Disc chip device suitable for blood sample pretreatment - Google Patents
Disc chip device suitable for blood sample pretreatment Download PDFInfo
- Publication number
- CN210803049U CN210803049U CN201921337913.7U CN201921337913U CN210803049U CN 210803049 U CN210803049 U CN 210803049U CN 201921337913 U CN201921337913 U CN 201921337913U CN 210803049 U CN210803049 U CN 210803049U
- Authority
- CN
- China
- Prior art keywords
- cavity
- pretreatment
- blood sample
- separation
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a disc chip device suitable for blood sample pretreatment; the device comprises a sample cavity, an extraction reagent cavity, a separation cavity, a purification filter membrane and a cavity for collecting sample filtrate; the device is designed based on a microfluidic chip for preprocessing, can be used for preprocessing various complex liquid detection materials by using a dispersive solid-phase extraction method such as QuECHERS (QuECHERS), reducing the matrix effect, realizing effective extraction of toxic drugs of blood samples and separation and purification of blood matrixes, directly obtaining analyzed samples, and being matched with an analysis measuring instrument for use, thereby realizing efficient, integrated, rapid and automatic drug analysis; the device performs sample pretreatment in a single channel and a cavity, is simple to operate, and can effectively reduce system errors and manual operation errors; the consumption is saved, the environment is protected, the space is saved, the pretreatment analysis device is efficient and environment-friendly, and the pretreatment analysis device can be widely applied to pretreatment of samples of forensic toxicants and clinical analyses.
Description
Technical Field
The utility model relates to a pretreatment field for toxic drug analysis, concretely relates to disc chip device and method suitable for blood sample preliminary treatment. The utility model discloses except being applicable to the blood sample, still include biological liquid samples such as urine, saliva.
Background
QuEChERS is a novel sample pretreatment method, and is widely applied to drug residue analysis due to the characteristics of quickness (quick), simplicity (easy), cheapness (snap), high efficiency (effective), reliability (rugged), safety (safe) and the like. The QuEChERS method was first proposed in 2003 in the united states and, after various validation and improvement, has demonstrated that over 200 pesticides can be detected using this method.
QuEChERS is essentially a derivative and further development of solid phase extraction technology and matrix solid phase dispersion technology. The basic principle of the method is that after a homogenized sample is extracted by acetonitrile (or acidified acetonitrile), extraction salt is adopted for salting out and layering, a matrix dispersion extraction mechanism is utilized, PSA (ethylenediamine-N-propylsilane) or other adsorbents such as C18 (octadecylsilane) and the like are combined with most of interferents (organic acid, fatty acid, carbohydrate and the like) in the matrix, and the interferents are removed in a centrifugal mode, so that the purification purpose is achieved.
For the analysis of target substances in a complex sample matrix, the QuEChERS pretreatment method is an efficient pretreatment method, can weaken the matrix effect in the sample analysis, and has been reported in a large number of documents in the analysis of drug residues in foods, environments and the like.
However, most of the processes of QuEChERS in biological samples are manual operations in steps, and no independent operation device or equipment applicable to the QuEChERS method exists.
On the other hand, the micro total analysis system combining micro electro mechanical technology and analytical chemistry detection, which is popular in the beginning of the 20 th century, can integrate all or part of basic operation units related to the fields of biology and chemistry, such as sample preparation, biological and chemical reaction, separation, detection, and the like, on a chip with a few square centimeters so as to complete different biological or chemical reaction processes.
Analysis and detection technologies based on micromachining technologies have been rapidly developed, wherein microfluidic chips have been widely applied to various aspects of clinical detection such as biochemical analysis, immunoassay, protein analysis, nucleic acid detection and the like, but have little application in the field of toxic drug analysis;
at present, reports on biological pretreatment chips only relate to chip research of liquid-liquid extraction and research reports on solid-phase extraction chips, but no report exists on application of the QuEChERS pretreatment technology to microfluidic chip analysis.
The utility model discloses based on the distinctive feature that integrates and high flux of micro-fluidic chip, designed the QuEChERS disc chip that can be applied to forensic medicine drug analysis, can integrate the QuEChERS method of multistep on single disc chip, easy operation is convenient. The utility model discloses can couple together biological sample preliminary treatment chip and subsequent detection and analytic process, can realize online analysis, be favorable to realizing portablely and automatic, reach the detection of high flux, realize little full analysis analytic system's target.
Disclosure of Invention
An object of the utility model is to overcome above-mentioned prior art's shortcoming and not enough, provide a disc chip device suitable for blood sample preliminary treatment.
The utility model discloses a following technical scheme realizes:
a disc chip device suitable for blood sample pretreatment comprises a microfluidic chip substrate and a pretreatment unit arranged on the microfluidic chip substrate.
The micro-fluidic chip base body is a rotatable circular base plate 1;
the number of the pretreatment units is at least two, the pretreatment units have the same structure, and are radially and symmetrically distributed on the circular base disc 1 by taking the circle center of the circular base disc 1 as the center;
each pretreatment unit comprises an extraction mixing cavity 11, a separation cavity 12, a purification cavity 13 and a filtration collection cavity 14;
the extraction mixing cavity 11, the separation cavity 12, the purification cavity 13 and the filtration and collection cavity 14 are sequentially arranged from the middle area to the edge area of the circular base plate 1;
the extraction mixing cavity 11 comprises a sample cavity 11-1, an extraction reagent pack 11-2 and a wave-shaped mixing channel 11-3;
the outlets of the sample cavity 11-1 and the extraction reagent pack 11-2 are communicated with the inlet end of the wavy mixing channel 11-3 through a flow channel; the outlet end of the wave-shaped mixing channel 11-3 is communicated with the separation cavity 12; the outlet of the separation cavity 12 is communicated with the purification cavity 13 through a first capillary pipeline 12-1, and the purification cavity 13 is communicated with the filtration and collection cavity 14 through a second capillary pipeline 13-1.
The separation chamber 12 and the purification chamber 13 are both in a long arc structure (fan-shaped or arc-shaped), wherein the volume of the separation chamber 12 is larger than that of the purification chamber 13.
The first capillary pipeline 12-1 is provided with an A bending detour part, and the vertex of the A bending detour part is higher than the B inner side part of the separation cavity 12; the second capillary pipeline 13-1 is provided with a C-shaped bent and circuitous part, and the vertex of the C-shaped bent and circuitous part is higher than the D inner side part of the purification cavity 13;
that is, when the centrifugal force is applied, the apex of the C-bend detour portion is lower than the liquid level in the purification chamber 13, and the apex of the a-bend detour portion is lower than the liquid level in the separation chamber 12;
the method specifically comprises the following steps: taking the center of the circular base plate 1 as a zero point, the distance from the inner side part B of the separation cavity 12 to the center of the circle is larger than the distance from the top point of the bending circuitous part A to the center of the circle; the distance from the inner side part D of the purifying cavity 13 to the circle center is larger than the distance from the top point of the C bending circuitous part to the circle center.
The outer edges of the two ends of the separation cavity 12 are respectively provided with a blood sample sedimentation matrix retaining cavity 12-2;
the purifying cavity 13 (the outer edge of the body) is provided with a purifying agent substrate retaining cavity 13-2.
The filtering and collecting cavity 14 is a strip arc cavity (fan-shaped or arc-shaped) and the volume of the filtering and collecting cavity is smaller than that of the purifying cavity 13. A counter bore for mounting a sample collection vial is provided at the end of the filtered collection chamber 14. The sample collection vial communicates with the end of the filtered collection chamber 14 through a cannula.
The separation cavity 12, the purification cavity 13 and the filtering and collecting cavity 14 are respectively provided with an exhaust duct 15.
The diameters of the first capillary channel 12-1 and the second capillary channel 13-1 are 100 and 300 μm. Preferably 100 μm.
A QuEChERS pretreatment method of a blood sample comprises the following steps:
the method comprises the following steps: sample loading extraction
Adding a blood sample into the sample cavity 11-1, fixing the extraction reagent pack 11-2 in the middle area of the circular base plate 1, alternately rotating the circular base plate 1 at forward and reverse rotating speeds which are set to be 200-3000 r/min, and extracting the blood sample for 1-2 min; under the action of centrifugal force, the blood sample in the sample cavity 11-1 and the extracting solution in the extracting reagent bag 11-2 are mixed and extracted through the wavy mixing channel 11-3;
step two: separation of
The mixed blood sample and the extracting solution enter a separation cavity 12, and after the mixed liquid in the separation cavity 12 is collected to a required volume, the rotating speed is set to be 3000 r/min-5000 r/min, and the duration time is 1-3 min; layering the mixed liquid under the action of centrifugal force to obtain an inner layer supernatant and an outer layer blood sample matrix, standing for 2-60 s, filling the supernatant into the first capillary pipeline 12-1 in 2-60 s due to siphonage, retaining the outer layer blood sample matrix in the blood sample precipitation matrix retaining cavity 12-2, and then giving centrifugal driving force at a rotating speed of 3000 r/min-5000 r/min for 5-60 s (the transfer time of other samples is related to viscosity); the supernatant enters the purifying cavity 13 to be separated from the blood sample matrix precipitated in the separating cavity 12;
in the inner layer supernatant and the outer layer blood sample matrix, the inner layer supernatant is the inner layer supernatant close to the center of the circular base plate 1; the outer blood sample matrix is the outer blood sample matrix close to the outer edge of the circular base plate 1.
Step three: purifying and filtering
The separated supernatant is driven in the purifying cavity 13 by the centrifugal force of the alternating rotation of the forward rotating speed and the reverse rotating speed, and is purified under the action of purifying agent particles preset in the purifying cavity 13; setting the rotating speed to be 200-3000 r/min, and carrying out sample purification for 30-90 s; then, layering the liquid and purifying agent particles under the driving of a centrifugal force at the rotating speed of 3000 r/min-5000 r/min for 1-3 min, wherein a purifying agent matrix is left in a purifying agent matrix retention cavity 13-2, the purifying agent is arranged on the outer layer close to the purifying cavity 13 (namely the direction of the outer edge of the circular base plate 1), and the purified liquid is arranged on the inner layer of the purifying cavity 13 (namely the direction of the center of the circular base plate 1);
step four: sample collection
The disc is stationary, purified liquid enters a second capillary pipeline 13-1 due to the siphoning effect, the second capillary pipeline 13-1 is filled with the liquid for 2-60 s, then centrifugal driving is performed, the rotating speed is 3000 r/min-5000 r/min, the centrifugal time is 5-60 s, and the liquid enters a filtering and collecting cavity 14 with a filter membrane for secondary filtering and sample collection;
the circular base plate 1 stops rotating, and the collected sample can be directly detected by the instrument.
Compared with the prior art, the utility model, following advantage and effect have:
(1) reducing matrix effect: the device of the utility model can be effectively used in the pretreatment process of the dispersed solid phase extraction such as QuECHERS, and can effectively reduce the matrix effect and improve the sensitivity of measuring the substance analysis by extracting, separating, purifying and secondarily purifying the sample of the complex matrix;
(2) integration: the utility model uses the chip as the platform to borrow the micro-processing technology, integrates a plurality of operations such as extraction, separation, purification and the like in the pretreatment process of the dispersed solid phase extraction such as QuECHERS and the like into the micro-channel, the cavity and other parts in the single circular base plate chip, and is convenient to integrate with other detection and analysis parts;
(3) high efficiency: the utility model adopts the multi-channel design, can simultaneously carry out the operations of extraction, separation, purification and the like of a plurality of samples in the circular basal disc chip, collect the filtrate and directly carry out the instrument analysis, can finish the treatment of a plurality of samples within 15 minutes, can realize the direct butt joint of the pretreatment operation and the instrument, and provides the technical basis for the intellectualization of the instrument;
the outer edges of the two ends of the separation cavity 12 of the utility model are respectively provided with a blood sample precipitation matrix retaining cavity 12-2, which can make the blood sample precipitation matrix with higher density stay in the blood sample precipitation matrix, better separate from the supernatant fluid on the inner side and avoid the capillary tube blockage caused by too long siphon time; the purifying agent substrate retaining cavity 13-2 is arranged at the outer edge of the purifying cavity 13 of the utility model, so that the purifying agent substrate with higher density can be retained in the purifying cavity, the purifying agent substrate can be better separated from the supernatant fluid on the inner side, and the capillary tube blockage caused by too long siphon time can be avoided;
(4) reduce errors in the analysis: the device can be effectively used for the pretreatment process of dispersed solid phase extraction such as QuECHERS, the appearance of an emulsion layer in liquid-liquid extraction can be avoided, and the human errors caused by system errors, manual liquid transfer and the like are reduced;
(5) the operation is simple: the utility model can be used for the pretreatment process of the dispersed solid phase extraction such as QuECHERS, and can be used for directly analyzing and detecting the filtrate after finishing the operations of extraction, separation, purification and the like in the dispersed solid phase extraction such as QuECHERS under the driving of single centrifugal force only by manually adding samples;
(6) green and environment-friendly: the device of the utility model can greatly reduce the analysis reagent, the consumable material and the like in the dispersed solid phase extraction process of QuECHERS and the like compared with the traditional method treatment, and avoid the loss of samples and the environmental pollution;
the utility model discloses the device, technological means simple accurate can use dispersion solid phase extraction methods such as QuECHERS to satisfy the preceding processing process of the extraction analysis of the complicated matrix of multiple liquid examine material poisoning medicine, can with the utility model discloses a device and analysis and determination instrument collocation use realize high-efficient, quick, automatic drug analysis.
Drawings
Fig. 1 is a schematic diagram of the structure of a disc chip device suitable for blood sample pretreatment.
Fig. 2 is a schematic structural view of the extraction mixing chamber 11 in fig. 1.
Fig. 3 is another schematic structural diagram of the separation chamber 12 and the purification chamber 13 in fig. 1.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments.
As shown in fig. 1-3, the present invention discloses a disc chip device suitable for blood sample pretreatment;
comprises a micro-fluidic chip matrix and a pretreatment unit arranged on the micro-fluidic chip matrix.
The micro-fluidic chip base body is a rotatable circular base plate 1;
the number of the pretreatment units is at least two, the pretreatment units have the same structure, and are radially and symmetrically distributed on the circular base disc 1 by taking the circle center of the circular base disc 1 as the center;
each pretreatment unit comprises an extraction mixing cavity 11, a separation cavity 12, a purification cavity 13 and a filtration collection cavity 14;
the extraction mixing cavity 11, the separation cavity 12, the purification cavity 13 and the filtration and collection cavity 14 are sequentially arranged from the middle area to the edge area of the circular base plate 1; the cleaning chamber 13 is filled with PSA or C18 filler particles.
The extraction mixing cavity 11 comprises a sample cavity 11-1, an extraction reagent pack 11-2 and a wave-shaped mixing channel 11-3;
the outlets of the sample cavity 11-1 and the extraction reagent pack 11-2 are communicated with the inlet end of the wavy mixing channel 11-3 through a flow channel; the outlet end of the wave-shaped mixing channel 11-3 is communicated with the separation cavity 12; the outlet of the separation cavity 12 is communicated with the purification cavity 13 through a first capillary pipeline 12-1, and the purification cavity 13 is communicated with the filtration and collection cavity 14 through a second capillary pipeline 13-1. The extraction reagent bag 11-2 contains acetonitrile mixed reagent.
The separation chamber 12 and the purification chamber 13 are both in a long arc structure (fan-shaped or arc-shaped), wherein the volume of the separation chamber 12 is larger than that of the purification chamber 13.
The first capillary pipeline 12-1 is provided with an A bending detour part, and the vertex of the A bending detour part is higher than the B inner side part of the separation cavity 12; the second capillary pipeline 13-1 is provided with a C-shaped bent and circuitous part, and the vertex of the C-shaped bent and circuitous part is higher than the D inner side part of the purification cavity 13;
that is, when the centrifugal force is applied, the apex of the C-bend detour portion is lower than the liquid level in the purification chamber 13, and the apex of the a-bend detour portion is lower than the liquid level in the separation chamber 12;
the method specifically comprises the following steps: taking the center of the circular base plate 1 as a zero point, the distance from the inner side part B of the separation cavity 12 to the center of the circle is larger than the distance from the top point of the bending circuitous part A to the center of the circle; the distance from the inner side part D of the purifying cavity 13 to the circle center is larger than the distance from the top point of the C bending circuitous part to the circle center.
The outer edges of the two ends of the separation cavity 12 are respectively provided with a blood sample sedimentation matrix retaining cavity 12-2;
the outer edge of the purifying cavity 13 is provided with a purifying agent substrate retaining cavity 13-2.
The filtering and collecting cavity 14 is a strip arc cavity, and the volume of the filtering and collecting cavity is smaller than that of the purifying cavity 13.
The separation cavity 12, the purification cavity 13 and the filtering and collecting cavity 14 are respectively provided with an exhaust duct 15.
The diameters of the first capillary channel 12-1 and the second capillary channel 13-1 are 100 and 300 μm. Preferably 100 mu m, the time for filling the capillary with the blood sample liquid is 2-60 s, the centrifugal liquid transfer time is within 5-60 s, and the other sample transfer times are related to the viscosity.
The utility model discloses blood sample's QuEChERS pretreatment method, the accessible is realized as follows step:
sample loading and extraction:
adding a blood sample into the sample cavity 11-1, fixing an extraction reagent pack 11-2 (such as a pressing reagent pack and an aluminum foil reagent pack) in the middle area of the circular base plate 1, alternately rotating the circular base plate 1 at forward and reverse rotating speeds which are set to be 200-3000 r/min, and extracting the blood sample for 1-2 min; under the action of centrifugal force, the blood sample in the sample cavity 11-1 and the extracting solution in the extracting reagent bag 11-2 are mixed and extracted through the wavy mixing channel 11-3;
separation of
The mixed blood sample and the extracting solution enter a separation cavity 12, and after the mixed liquid in the separation cavity 12 is collected to a required volume, the rotating speed is set to be 3000 r/min-5000 r/min, and the duration time is 1-3 min; layering the mixed liquid under the action of centrifugal force to obtain an inner layer supernatant and an outer layer blood sample matrix, standing for 2-60 s, filling the capillary pipeline 12-1 in the supernatant in 2-60 s due to siphonage, retaining the outer layer blood sample matrix in the blood sample precipitation matrix retaining cavity 12-2, and then giving centrifugal driving force at a rotating speed of 3000 r/min-5000 r/min for 5-60 s (the transfer time of other samples is related to viscosity). The supernatant enters the purifying cavity 13 to be separated from the blood sample matrix precipitated in the separating cavity 12;
obtaining inner layer supernatant and outer layer blood sample matrix, wherein the inner layer supernatant is the inner layer supernatant close to the center direction of the circular base plate 1; the outer blood sample matrix is the outer blood sample matrix close to the outer edge of the circular base plate 1.
Purifying and filtering
The separated supernatant is driven in the purifying cavity 13 by the centrifugal force of the alternating rotation of the forward rotating speed and the reverse rotating speed, and is purified under the action of purifying agent particles preset in the purifying cavity 13; setting the rotating speed to be 200-3000 r/min, and carrying out sample purification for 30-90 s; then the liquid and the purifying agent particles are layered under the driving of the centrifugal force at the rotating speed of 3000 r/min-5000 r/min, the centrifugal time is 1-3 min, and the purifying agent matrix is left in the purifying agent matrix retention cavity 13-2. The outer layer (namely the outer edge direction of the circular base plate 1) close to the purifying cavity 13 is a purifying agent, and the inner layer (namely the center direction of the circular base plate 1) of the purifying cavity 13 is purified liquid;
sample collection
And (3) standing the disc, enabling the purified liquid to enter a second capillary pipeline 13-1 under the siphoning action, filling the capillary for 2-60 s, then carrying out centrifugal driving at the rotating speed of 3000-5000 r/min for 5-60 s, and enabling the liquid to enter a filtering and collecting cavity 14 with a filter membrane for secondary filtering and sample collection.
A counterbore may be provided at the end of the filtered collection chamber 14 for receiving a sample collection vial. The sample collection bottle is communicated with the tail end of the filtering and collecting cavity 14 through a cannula to collect the sample.
The circular base plate 1 stops rotating, collected samples can be directly detected by an instrument, wherein the blood samples are whole blood, plasma and serum, and the device can also be used for carrying out pretreatment analysis on medicines in biological samples such as urine, saliva and the like. The medicine is abuse medicine such as listed amphetamine class medicine, also can use this device to carry out other kinds of pharmaceutical analysis of complicated matrix sample, other analysis detection such as pesticide and veterinary drug residue, mycotoxin also can use the utility model discloses a pretreatment analysis is carried out to the device.
The volume of the sample cavity 11-1 of the utility model is 200 muL-400 muL, the volume of the reagent cavity for placing the extraction reagent pack 11-2 is 1000 muL-1500 muL, and the reagent cavity or the reagent pack comprises 4-6 grinding beads; 20-40 mg of packing such as PSA, 10mg of C18 and the like are placed in the purification cavity 13, the volume of the cavity is 1200-2000 mu L, a filter screen is arranged at the cavity outlet of the purification cavity 13, and the filter collection cavity 14 is also provided with an organic filter membrane of 0.22 mu m.
As described above, the utility model can be realized well, and the shape and the size of the cavity can be modified for the utility model.
The embodiments of the present invention are not limited by the above-mentioned embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be equivalent replacement modes, and all are included in the scope of the present invention.
Claims (8)
1. The utility model provides a disc chip device suitable for blood sample preliminary treatment, includes the micro-fluidic chip base member to and establish the pretreatment unit on the micro-fluidic chip base member, its characterized in that:
the micro-fluidic chip substrate is a rotatable circular substrate (1);
the number of the pretreatment units is at least two, the pretreatment units have the same structure, and are radially and symmetrically distributed on the circular base disc (1) by taking the circle center of the circular base disc (1) as the center;
each pretreatment unit comprises an extraction mixing cavity (11), a separation cavity (12), a purification cavity (13) and a filtration collection cavity (14);
the extraction mixing cavity (11), the separation cavity (12), the purification cavity (13) and the filtration collection cavity (14) are sequentially arranged from the middle area to the edge area of the circular base plate (1);
the extraction mixing cavity (11) comprises a sample cavity (11-1), an extraction reagent bag (11-2) and a wave-shaped mixing channel (11-3);
the outlets of the sample cavity (11-1) and the extraction reagent bag (11-2) are communicated with the inlet end of the wavy mixing channel (11-3) through a flow channel; the outlet end of the wave-shaped mixing channel (11-3) is communicated with the separation cavity (12); the outlet of the separation cavity (12) is communicated with the purification cavity (13) through a first capillary pipeline (12-1), and the purification cavity (13) is communicated with the filtering and collecting cavity (14) through a second capillary pipeline (13-1).
2. The disc chip device for the pretreatment of blood sample according to claim 1, wherein:
the first capillary pipeline (12-1) is provided with an A bending detour part, and the vertex of the A bending detour part is higher than the B inner side part of the separation cavity (12); the second capillary pipeline (13-1) is provided with a C-shaped bent and circuitous part, and the vertex of the C-shaped bent and circuitous part is higher than the D inner side part of the purification cavity (13);
namely, when the centrifugal force is applied, the top of the C bending and winding part is lower than the liquid level in the purification cavity (13), and the top of the A bending and winding part is lower than the liquid level in the separation cavity (12);
the method specifically comprises the following steps: taking the circle center of the circular base plate (1) as a zero point, wherein the distance from the inner side part B of the separation cavity (12) to the circle center is greater than the distance from the top point of the bending circuitous part A to the circle center; the distance from the D inner side part of the purification cavity (13) to the circle center is larger than the distance from the top point of the C bending circuitous part to the circle center.
3. The disc chip device for blood sample pretreatment according to claim 2, wherein: the outer edges of the two ends of the separation cavity (12) are respectively provided with a blood sample sedimentation matrix retaining cavity (12-2);
the outer edge of the purifying cavity (13) is provided with a purifying agent substrate retaining cavity (13-2).
4. The disc chip device for the pretreatment of blood sample according to claim 3, wherein: the separation chamber (12) and the purification chamber (13) are both in a strip arc structure, wherein the volume of the separation chamber (12) is larger than that of the purification chamber (13).
5. The disc chip device for the pretreatment of blood sample according to claim 3, wherein: the filtering and collecting cavity (14) is a strip arc cavity, and the volume of the filtering and collecting cavity is smaller than that of the purifying cavity (13).
6. The disc chip device for the pretreatment of blood sample according to claim 3, wherein: the separation cavity (12), the purification cavity (13) and the filtering and collecting cavity (14) are respectively provided with an exhaust duct (15).
7. The disc chip device for the pretreatment of blood sample according to claim 3, wherein: the tube diameters of the first capillary channel (12-1) and the second capillary channel (13-1) are 100-300 μm.
8. The disc chip device for the pretreatment of blood sample according to claim 3, wherein: and a counter bore for installing a sample collecting bottle is arranged at the tail end of the filtering and collecting cavity (14), and the sample collecting bottle is communicated with the tail end of the filtering and collecting cavity (14) through a cannula.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921337913.7U CN210803049U (en) | 2019-08-16 | 2019-08-16 | Disc chip device suitable for blood sample pretreatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921337913.7U CN210803049U (en) | 2019-08-16 | 2019-08-16 | Disc chip device suitable for blood sample pretreatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210803049U true CN210803049U (en) | 2020-06-19 |
Family
ID=71225396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921337913.7U Active CN210803049U (en) | 2019-08-16 | 2019-08-16 | Disc chip device suitable for blood sample pretreatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210803049U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441108A (en) * | 2019-08-16 | 2019-11-12 | 华南理工大学 | One kind being suitable for the pretreated disk chip apparatus of blood sample and method |
-
2019
- 2019-08-16 CN CN201921337913.7U patent/CN210803049U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441108A (en) * | 2019-08-16 | 2019-11-12 | 华南理工大学 | One kind being suitable for the pretreated disk chip apparatus of blood sample and method |
| CN110441108B (en) * | 2019-08-16 | 2024-05-07 | 华南理工大学 | Disc chip device and method suitable for pretreatment of blood sample |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3989964B2 (en) | Integrated microfluidic device | |
| US6695147B1 (en) | Absorption-enhanced differential extraction device | |
| EP3058365B1 (en) | Microfluidics sorter for cell detection and isolation | |
| JP4869602B2 (en) | Method and apparatus for dividing a specimen into multiple channels of a microfluidic device | |
| US6432630B1 (en) | Micro-flow system for particle separation and analysis | |
| EP3523003B1 (en) | Particle isolation/enrichment using continuous closed-loop micro-fluidics | |
| US9696299B2 (en) | Integrated modular unit including an analyte concentrator microreactor device connected to a cartridge-cassette | |
| JP2002502597A (en) | Integrated microfluidic device | |
| WO1997047390A9 (en) | Absorption-enhanced differential extraction device | |
| US10518196B2 (en) | Devices for separation of particulates, associated methods and systems | |
| WO2003044481A3 (en) | Optical bio-discs and microfluidic devices for analysis of cells | |
| KR20150018774A (en) | A test cartridge with integrated transfer module | |
| CA2703950A1 (en) | Apparatus, system, and method for purifying nucleic acids | |
| JPH0657324B2 (en) | Pipette tip, analyzer and liquid separation method | |
| Kim et al. | Microfluidic pipette tip for high-purity and high-throughput blood plasma separation from whole blood | |
| CN103175975B (en) | For antipollution method | |
| CN110441108B (en) | Disc chip device and method suitable for pretreatment of blood sample | |
| CN103293050A (en) | Serum filter chip and preparation chip thereof | |
| EP2838636A1 (en) | Microfilter and apparatus for separating a biological entity from a sample volume | |
| CN210803049U (en) | Disc chip device suitable for blood sample pretreatment | |
| WO2013021101A1 (en) | Method and apparatus for automated analysis | |
| EP2742354A1 (en) | Method and apparatus for automated analysis | |
| CN207012992U (en) | A kind of microfluidic devices of Trace bio-element, detection excretion body | |
| US11927600B2 (en) | Fluidic bridge device and sample processing methods | |
| Shen et al. | High-throughput blood plasma extraction in a dimension-confined double-spiral channel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |