CN209974767U

CN209974767U - Immune cell washs centrifuging tube

Info

Publication number: CN209974767U
Application number: CN201920308209.2U
Authority: CN
Inventors: 丁星; 张学勇; 丁乐石
Original assignee: Shenzhen North Science And Technology Co Ltd
Current assignee: Shenzhen North Science And Technology Co Ltd
Priority date: 2019-03-12
Filing date: 2019-03-12
Publication date: 2020-01-21
Anticipated expiration: 2029-03-12

Abstract

The utility model discloses an immune cell washs centrifuging tube, including centrifuging tube body and the sealed cap of setting in centrifuging tube body upper end, the bottom of centrifuging tube body be the toper structure, the intermediate position department of sealed cap be provided with the pipette, the lower extreme of pipette extends to centrifuging tube body bottom and just guarantees contactless sample cell apart from the distance of centrifuging tube body bottom surface always, pipette upper end be provided with sealed glue, one side of sealed cap on be equipped with the respiratory orifice, the respiratory orifice and the upper and lower both ends intercommunication and the respiratory orifice of sealed cap in fill and have aseptic filter material. The utility model discloses an immune cell washs centrifuging tube, can be quick wash immune cell, can operate in ordinary environment, need not establish special aseptic laboratory, just can go on in the outpatient service, prepares out the immune cell that meets the requirements.

Description

Immune cell washs centrifuging tube

Technical Field

The utility model relates to an experimental apparatus technical field, in particular to immune cell washs centrifuging tube.

Background

With the rise of CAR-T treatment technology, how to rapidly extract immune cells from human peripheral blood for culture becomes an important work for restricting immune cell treatment technology, and the extraction of immune cells is generally separated by adopting a density gradient method or a natural sedimentation method. However, regardless of the separation technique, the separated immune cells need to be washed for the second time to remove impurities, so as to provide clean cells for subsequent cryopreservation and culture, which takes a long time and affects the washing efficiency.

SUMMERY OF THE UTILITY MODEL

The main objective of the utility model is to provide an immune cell washs centrifuging tube to the realization is to immune cell's quick washing.

In order to realize the above-mentioned purpose, the utility model provides an immune cell washs centrifuging tube, including centrifuging tube body and the sealed cap of setting in centrifuging tube body upper end, the bottom of centrifuging tube body be the toper structure, the intermediate position department of sealed cap be provided with the pipette, the upper end of pipette is located the intermediate position of sealed cap, the lower extreme extends to centrifuging tube body bottom and just guarantees contactless sample cell apart from the distance of centrifuging tube body bottom surface always, pipette upper end be provided with sealed glue, sealed lower extreme surface and the upper end surface in close contact with of pipette, its upper end surface is flat mutually with the upper end surface of sealed cap, one side of sealed cap on be equipped with the breather hole, upper and lower both ends intercommunication and the breather hole of sealed cap in fill and have aseptic filter material.

Preferably, the caliber of the upper end opening of the pipette is larger than the caliber of the lower end opening, and the cross section of the pipette is in a shape of a Chinese character 'tu'.

Preferably, a sealing ring is arranged between the sealing cap and the centrifuge tube body.

Preferably, the centrifuge tube body adopts compound GMP material.

Preferably, the sterile filter material is a sterile filter element.

The utility model discloses an immune cell washs centrifuging tube, can be quick wash immune cell, can operate in ordinary environment, need not establish special aseptic laboratory, just can go on in the outpatient service, prepares out the immune cell that meets the requirements.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

Fig. 1 is a schematic structural view of an immune cell cleaning centrifuge tube provided by the embodiment of the present invention.

The reference numbers illustrate:

reference numerals	Name (R)	Reference numerals	Name (R)
				1	Centrifuge tube body	2	Sealing cap
3	Pipette with a suction tube	4	Sealing glue
				5	Breathing hole	6	Sterile filter material
7	Sealing ring

The objects, features and advantages of the present invention will be further described with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.

In addition, the descriptions related to "first", "second", etc. in the present invention are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit ly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

The embodiment of the utility model provides an in, refer to figure 1, this immune cell washs centrifuging tube, including centrifuging tube body 1 and the sealed cap 2 of setting in centrifuging tube body 1 upper end, sealed cap 2 and centrifuging tube body 1 between be provided with sealing washer 7, can effectually prevent that external pollutant from entering into centrifuging tube body 1. The centrifuge tube body 1 is made of composite GMP materials and has no biological pollution to samples.

Furthermore, the bottom of the centrifuge tube body 1 is in a conical structure, so that sedimentation and collection of cells are facilitated. The middle position department of sealed cap 2 be provided with pipette 3, the upper end of pipette 3 is located the middle position of sealed cap 2, the lower extreme extends to 1 bottom of centrifuging tube body and just guarantees not to contact sample cell apart from the distance of 1 bottom surface of centrifuging tube body always, the upper end open-ended bore of pipette 3 is greater than lower extreme open-ended bore, and the shape of its cross section is "protruding" font.

Furthermore, the upper end of the pipette 3 is provided with a sealing glue 4, the lower end surface of the sealing glue 4 is in close contact with the upper end surface of the pipette 3, the upper end surface of the sealing glue 4 is level with the upper end surface of the sealing cap 2, the sealing glue 4 can be punctured by a syringe and has certain elasticity, and the sealing performance during the puncturing process of the syringe and after the needle of the syringe is pulled out is ensured.

In this embodiment, one side of sealed cap 2 on be equipped with respiratory orifice 5, respiratory orifice 5 and sealed cap 2 the upper and lower both ends intercommunication and respiratory orifice 5 in fill and have aseptic filter material 6, aseptic filter material 6 be aseptic filter core, can prevent that external pollutants from getting into the inside of centrifuging tube body 1 through expiratory hole.

The working process of this embodiment is as follows:

1. injecting the separated immune cell suspension into a centrifuge tube body through an injector;

2. injecting normal saline into the syringe, and pumping for several times to ensure uniform mixing of the liquid;

3. placing the centrifuge tube body on a centrifuge for centrifugation to precipitate cells;

4. standing the centrifuge tube body vertically, and extracting the upper layer liquid by using an injector;

5. repeating the steps until the cell compounding requirement is met;

6. when the cells need to be cryopreserved, injecting the cryopreserving liquid by using an injector, pumping for a plurality of times to uniformly mix the liquid, pumping out the cells needing to be cryopreserved, and transferring the cells into a cryopreserving tube for cryopreservation.

The above only be the preferred embodiment of the utility model discloses a not consequently restriction the utility model discloses a patent range, all are in the utility model discloses a conceive, utilize the equivalent structure transform of what the content was done in the description and the attached drawing, or direct/indirect application all is included in other relevant technical field the utility model discloses a patent protection within range.

Claims

1. The utility model provides an immune cell washs centrifuging tube, includes centrifuging tube body and the sealed cap of setting in centrifuging tube body upper end, a serial communication port, the bottom of centrifuging tube body be the toper structure, the intermediate position department of sealed cap be provided with the pipette, the upper end of pipette is located the intermediate position of sealed cap, the lower extreme extends to centrifuging tube body bottom and just guarantees contactless sample cell apart from the distance of centrifuging tube body bottom surface always, pipette upper end be provided with sealed glue, sealed lower extreme surface and the upper end surface in close contact with of pipette, its upper end surface is flat with the upper end surface of sealed cap mutually, one side of sealed cap on be equipped with the breather hole, upper and lower both ends intercommunication and the breather hole of sealed cap in fill and have aseptic filter material.

2. The immune cell cleaning centrifuge tube according to claim 1, wherein the pipette has an upper opening with a diameter larger than that of a lower opening, and has a cross-section in a shape of a "convex".

3. The immune cell washing centrifuge tube of claim 1, wherein a sealing ring is disposed between the sealing cap and the centrifuge tube body.

4. The immune cell cleaning centrifuge tube of claim 1, wherein the centrifuge tube body is made of GMP composite material.

5. The immune cell cleaning centrifuge tube of claim 1, wherein the sterile filter material is a sterile filter element.