CN206008161U - A kind of cell filter - Google Patents
A kind of cell filter Download PDFInfo
- Publication number
- CN206008161U CN206008161U CN201620879010.1U CN201620879010U CN206008161U CN 206008161 U CN206008161 U CN 206008161U CN 201620879010 U CN201620879010 U CN 201620879010U CN 206008161 U CN206008161 U CN 206008161U
- Authority
- CN
- China
- Prior art keywords
- rack body
- cell filter
- drainage screen
- test tube
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
This utility model belongs to biological technical field, and in particular to a kind of cell filter;Which includes that generally columnar rack body, one end of rack body are fixedly installed support apical ring, and the external diameter of support apical ring is more than the external diameter of rack body;The other end of rack body is fixedly installed the drainage screen of inclination;Hydrophilic oxidant layer is provided with drainage screen.This utility model can make cell suspension sample easily overcome surface tension, may pass through drainage screen by gravity downstream to test tube bottom, and without the need for being suspended by centrifugation again or realizing filtering by other external force;Test tube inner air flow is may further ensure that, so that the cell suspension sample in rack body more successfully can be filtered;Cell filter is allow to be more easily inserted into or remove test tube, easy to operate.
Description
Technical field
This utility model belongs to biological technical field, and in particular to a kind of cell filter.
Background technology
Cell filtration is the biological necessary means of regular growth;Which is used for impurity screening, excludes AC, sorting not
Same sized particles.
Requirement of the Flow Cytometry to cell filtration is particularly stringent;The flow cytometer ratio used by Flow Cytometry
More accurate and complex structure, the particle requirement to sample are high, and excessive impurity or AC can all cause flow cytometer
The blocking of liquid fluid system.In the event of blocking, cleaning every time is required for consuming one hour or longer flow cytometer liquid fluid system
Time, substantial amounts of human and material resources and financial resources can be wasted.
Have some cell filters on the market at present, but these cell filters are suffered from the drawback that:
1st, cell suspension sample cannot natural filtration, be often required to by centrifugation-suspend again or by the stirring of other external force
Could realize filtering;Cumbersome and be easily destroyed cell;When some occur than more serious situation, cell fatigue can be caused
So as to cause necrocytosiss or natural death of cerebral cells, experimental result is had a strong impact on;
2nd, the support aperture of cell filter and test tube are mismatched, and make cell filter seemingly cannot be inserted tightly into test tube,
Filtrate is caused easily to be spilt;It is typically necessary the other transfer steps of increase and does not spill ensureing filtrate, causes to waste time and energy;
3rd, generally conventional metal is made, and manufacturing cost is high, and cannot use gamma sterilization.
Utility model content
For solving the above problems, the purpose of this utility model is to provide a kind of cell filter, i.e. can be with without the need for external force
Realize filtering, and easy to operate.
For achieving the above object, drainage screen of the present utility model is inclined and is provided with hydrophilic oxidant layer, hangs cell
Liquid sample can overcome surface tension, so as to can achieve to filter without the need for external force.In addition, this utility model is set on rack body
Support ring is equipped with, finger can be put into space between support ring and support apical ring, thin such that it is able to easily take out from test tube
Born of the same parents' filter;The reinforcement for positioning is provided with rack body, allows cell filter easily, approximately closely
Insertion test tube.
The detailed technology scheme that this utility model is taken is:A kind of cell filter, it include generally columnar
Frame body, one end of rack body are fixedly installed support apical ring, and the external diameter of support apical ring is more than the external diameter of rack body;Support
The other end of main body is fixedly installed the drainage screen of inclination;Hydrophilic oxidant layer is provided with drainage screen.
Further, rack body is further fixedly arranged on support ring;Support ring is arranged on the lower section of support apical ring.Finger can
To be put into space between support ring and support apical ring, such that it is able to easily take out cell filter from test tube.
Further, the breach for running through is additionally provided with support ring.Test tube inner air flow can further be ensured, from
And the cell suspension sample in rack body is more successfully filtered.
Further, the outer wall of rack body is further fixedly arranged at least three reinforcements.Positioning can be played a part of,
Cell filter is made approximately to be inserted tightly into test tube, it is ensured that test tube inner air flow, so that the cell in cell filter is outstanding
Liquid sample more successfully can be filtered, and also cell filter can be made easily to be inserted or removed from test tube.
Further, the section of reinforcement is triangle.Reinforcement can be reduced to subtract with the contact area of inboard wall of test tube
Less friction, so that cell filter can be more easily inserted into or remove test tube, easy to operate.
Further, the outer wall of rack body is provided with color coating.By distinguishing different colors, area can be facilitated
Divide the filter of the drainage screen of different pore size
Further, rack body is made up of polystyrene or rustless steel.Rack body is made for polystyrene and then may be used
To be sterilized using gamma sterilization, more convenient;Rack body is made for rustless steel and can then adopt conventional laboratory height
Warm autoclaving, reaches reusable purpose.
Further, drainage screen is made up of nylon or rustless steel.Drainage screen is made for nylon, can be penetrated using gamma
Line sterilization, more convenient;Drainage screen is made for rustless steel and then can adopt conventional laboratory autoclave sterilization, reaches
Reusable purpose.
Further, the inclination angle of drainage screen is 30 degree or 45 degree or 60 degree.
Compared with prior art, this utility model has the advantages that:1st, cell suspension sample can easily gram
Surface tension is taken, and drainage screen is may pass through downstream to test tube bottom by gravity, and without the need for by centrifugation-suspension or dependence again
Other external force are realizing filtering;2nd, test tube inner air flow can further be ensured, so that the cell in rack body is outstanding
Liquid sample more successfully can be filtered;3rd, cell filter is allow to be more easily inserted into or remove test tube, operation side
Just.
Description of the drawings
Fig. 1 is a kind of schematic side view of the cell filter in this utility model.
Schematic side view when Fig. 2 is a kind of cell filter insertion test tube in this utility model.
Fig. 3 is the schematic diagram of A-A sections.
Rack body 1;Support apical ring 11;Drainage screen 12;Support ring 13;Reinforcement 14;Breach 15;Test tube 2;Inclination angle B.
Specific embodiment
Fig. 1-Fig. 3 is referred to, this utility model is a kind of cell filter, and which includes generally columnar rack body
1, one end of rack body 1 is fixedly installed support apical ring 11, and the external diameter of support apical ring 11 is more than the external diameter of rack body 1;?
The other end (i.e. the other end of one end for being provided with support apical ring 11 of rack body 1) of frame body 1 is fixedly installed inclination
Drainage screen 12 (i.e. the inclination angle B of drainage screen 12 is more than zero degree);Hydrophilic oxidant layer is provided with drainage screen 12.
A kind of operation principle of the cell filter in this utility model is:When needing using cell filter, by cell
In filter insertion test tube 2, cell suspension sample is added cell filter lentamente then.
As the other end of rack body 1 is fixedly installed the drainage screen 12 of inclination;Hydrophilizing agent is provided with drainage screen 12
Layer;Cell suspension sample can easily overcome surface tension, may pass through drainage screen 12 downstream to 2 bottom of test tube by gravity
Portion, and without the need for by being centrifuged-suspending again or realize filtering by other external force.
When cell suspension sample is all added in cell filter, and after the completion of cell suspension sample is all filtered, from
In test tube 2 extract out cell filter and as needed process cell filter (such as can by cell filter make rubbish abandon,
To can also reuse after cell filter cleaning and sterilizing).Cell suspension sample in test tube 2 then can be used for next step
Experiment, such as FCM analysis experiment.
It is, of course, also possible to applying positive air pressure into cell filter (is fixedly installed support apical ring from cell filter
The opening of 11 one end, applies air pressure to the direction towards drainage screen 12), to accelerate the rate of filtration.
Rack body 1 can also be fixedly installed support ring 13;Support ring 13 is arranged on the lower section of support apical ring 11.Will be thin
When born of the same parents' filter is taken out from test tube 2, finger can be put into space between support ring 13 and support apical ring 11, such that it is able to convenient
Cell filter is taken out from test tube 2 in ground.
The breach 15 for running through is also provided with support ring 13.Interior for test tube 2 and extraneous UNICOM can be entered one by breach 15
Step ground ensures 2 inner air flow of test tube, so that the cell suspension sample in rack body 1 more successfully can be filtered.
The outer wall of rack body 1 can also be fixedly installed at least three reinforcements 14.Three reinforcements 14 can be played
The effect of positioning, makes approximate closely (i.e. the outer wall of rack body 1 has gap with the inwall of the test tube 2) insertion of cell filter
Test tube 2, it is ensured that 2 inner air flow of test tube, so that the cell suspension sample in cell filter more successfully can be filtered,
And also cell filter can be made easily to be inserted or removed from test tube 2.
The section of reinforcement 14 can be triangle.The contact area of reinforcement 14 and 2 inwall of test tube can be reduced and subtracted
Less friction, so that cell filter can be more easily inserted into or remove test tube 2, easy to operate.
The outer wall of rack body 1 can be provided with color coating.By distinguishing different colors, easily can distinguish not
Filter with the drainage screen 12 in aperture;For example the cell filter of the corresponding 40um bore filters net 12 of red coatings, yellow are applied
The cell filter of the corresponding 75um drainage screens 12 of layer, the cell filter of the corresponding 100um drainage screens 12 of green coating;To adapt to not
The needs of same test tube 2 (for example FCM analysis pipe).
Rack body 1 can be made up of polystyrene or rustless steel.Rack body 1 is made for polystyrene, can be adopted
Sterilized with gamma sterilization, more convenient;Rack body 1 is made for rustless steel and can then adopt conventional laboratory high temperature height
Pressure sterilizing, reaches reusable purpose.
Drainage screen 12 can be made up of nylon or rustless steel.Drainage screen 12 is made for nylon and can then adopt gamma ray
Sterilization, more convenient;Drainage screen 12 is made for rustless steel and then can adopt conventional laboratory autoclave sterilization, reaches
Reusable purpose.
The inclination angle B of drainage screen 12 can be 30 degree or 45 degree or 60 degree.
It should be noted that those skilled in the art are easily derived according to above-described embodiment, the height of cell filter
(i.e. support apical ring 11 upper surface to the lowermost end of drainage screen 12 distance) be different numerical value embodiment, or drainage screen 12
Inclination angle B be other numerical value embodiment;In order to avoid unnecessary repetition, below repeat no more, but without departing from this practicality
New protection domain.
In sum, a kind of cell filter in this utility model, by gravity may pass through drainage screen 12 downstream to
2 bottom of test tube, and without the need for by being centrifuged-suspending again or realize filtering by other external force;Can ensure that air in test tube 2
Circulation, so that the cell suspension sample in cell filter more successfully can be filtered, and also can make cell filtration
Device is easily inserted or removed from test tube 2.
It is to be appreciated that term " " center ", " longitudinal direction ", " horizontal ", "front", "rear", "left", "right", " vertical ", " water
Flat ", " top ", " bottom ", " interior ", the orientation of instruction such as " outward " or position relationship be based on orientation shown in the drawings or position relationship,
Only it is to describe with simplifying for ease of the description present invention, rather than indicates or infer the device or element of indication must have specifically
Orientation, for specific azimuth configuration and operation, thus it is not intended that restriction to present invention protection content.
Above specific embodiment is only the preferred embodiment of this creation, not in order to limit this creation, all in this wound
Any modification, equivalent substitution and improvements for being done etc. within the spirit of work and principle, should be included in this creation protection domain it
Interior.
Claims (9)
1. a kind of cell filter, it is characterised in that:Including generally columnar rack body (1), the one of rack body (1)
End is fixedly installed support apical ring (11), and the external diameter of support apical ring (11) is more than the external diameter of rack body (1);Rack body (1)
The other end be fixedly installed the drainage screen (12) of inclination;Drainage screen is provided with hydrophilic oxidant layer on (12).
2. a kind of cell filter as claimed in claim 1, it is characterised in that:Rack body (1) is further fixedly arranged on
Support ring (13);Support ring (13) is arranged on the lower section of support apical ring (11).
3. a kind of cell filter as claimed in claim 2, it is characterised in that:It is additionally provided with support ring (13) and runs through
Breach (15).
4. a kind of cell filter as claimed in claim 1, it is characterised in that:The outer wall of rack body (1) is also fixed
It is provided with least three reinforcements (14).
5. a kind of cell filter as claimed in claim 4, it is characterised in that:The section of reinforcement (14) is triangle
Shape.
6. a kind of cell filter as described in claim 1-5 is arbitrary, it is characterised in that:The outer wall of rack body (1)
It is provided with color coating.
7. a kind of cell filter as described in claim 1-5 is arbitrary, it is characterised in that:Described rack body (1) is poly- third
Ethylene or rustless steel are made.
8. a kind of cell filter as described in claim 1-5 is arbitrary, it is characterised in that:Described drainage screen (12) be nylon or
Person's rustless steel is made.
9. a kind of cell filter as described in claim 1-5 is arbitrary, it is characterised in that:The inclination angle of drainage screen (12)
For 30 degree or 45 degree or 60 degree.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201620879010.1U CN206008161U (en) | 2016-08-14 | 2016-08-14 | A kind of cell filter |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201620879010.1U CN206008161U (en) | 2016-08-14 | 2016-08-14 | A kind of cell filter |
Publications (1)
Publication Number | Publication Date |
---|---|
CN206008161U true CN206008161U (en) | 2017-03-15 |
Family
ID=58247335
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201620879010.1U Expired - Fee Related CN206008161U (en) | 2016-08-14 | 2016-08-14 | A kind of cell filter |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN206008161U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114126741A (en) * | 2019-09-09 | 2022-03-01 | 株式会社村田制作所 | Concentration device and concentration method |
-
2016
- 2016-08-14 CN CN201620879010.1U patent/CN206008161U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114126741A (en) * | 2019-09-09 | 2022-03-01 | 株式会社村田制作所 | Concentration device and concentration method |
CN114126741B (en) * | 2019-09-09 | 2024-02-06 | 株式会社村田制作所 | Concentrating device and concentrating method |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200729 Address after: Unit 601, block 1, No. 116, Gulou Road, Gulou District, Fuzhou City, Fujian Province Patentee after: Gui Jinpeng Address before: 100041 Beijing, Shijingshan District, West Wells Road, No. 19, building 6, floor 3, 601 Patentee before: BEIJING IVY BIOMED MEDICAL SCIENCE TECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170315 Termination date: 20200814 |
|
CF01 | Termination of patent right due to non-payment of annual fee |