CN209826716U - Experimental rat myocardial cell action potential acquisition device - Google Patents
Experimental rat myocardial cell action potential acquisition device Download PDFInfo
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- CN209826716U CN209826716U CN201920020552.7U CN201920020552U CN209826716U CN 209826716 U CN209826716 U CN 209826716U CN 201920020552 U CN201920020552 U CN 201920020552U CN 209826716 U CN209826716 U CN 209826716U
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- needle
- action potential
- puncture
- puncture needle
- butt joint
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Abstract
The utility model belongs to animal experiment apparatus field, in particular to experiment rat cardiac muscle cell action potential collection system. The utility model comprises a puncture needle and a butt joint fixer, wherein the puncture needle is inserted into the butt joint fixer; the acquisition method is characterized in that action potential signals of ventricular muscles are acquired by matching the puncture needle with the butt joint fixator. The utility model provides a current conventional rat cardiac muscle cell action potential need open the thorax and gather, require high to operation gimmick and time, the time is long slightly then the rat heart stops beating, can not gather the action potential scheduling problem of cardiac muscle, develops a collection system and a method that need not open the rat thorax, can gather the action potential of cardiac muscle.
Description
Technical Field
The utility model belongs to animal experiment apparatus field, in particular to experiment rat cardiac muscle cell action potential collection system.
Background
Animal experiments provide theoretical basis for medical science progress, and action potential of myocardial cells is the basis for researching various pathogenic factors causing electrocardiogram change. The conventional rat myocardial cell action potential is collected by opening the chest, the operation process is required to be rapid and skilled, the time is a little longer, and the myocardial action potential cannot be collected when the heart stops beating.
Disclosure of Invention
Utility model purpose:
the utility model discloses aim at providing experiment rat cardiac muscle cell action potential collection system, under the condition of not opening the rat thorax, gather cardiac muscle cell's action potential.
The technical scheme is as follows:
experimental rat cardiac muscle cell action potential collection system, collection system include pjncture needle and butt joint fixer, and the pjncture needle is pegged graft with butt joint fixer.
The lower end of the puncture needle which is integrally made of steel is provided with a needle point, a reversed water-drop-shaped insulator is fixed above the needle point, and a needle handle is arranged on the puncture needle; the upper end of the puncture needle is provided with a lead connected with an electrocardiosignal collector.
The puncture needle has a needle length of 30 ~ 40 mm, a needle point length of 0.3 ~ 0.5.5 mm, a short diameter of 0.5 ~ 1.0.0 mm and a long diameter of 2.0 ~ 3.0.0 mm of inverted water drop-shaped insulator above the needle point, a length of 2.5 ~ 4.0.0 mm in the vertical direction of the water drop, an oval horizontal plane of the needle handle, a trapezoidal crown plane and a trapezoidal sagittal plane.
The long axis of the upper elliptical surface of the needle handle is 8 ~ 12 mm, the short axis of the upper elliptical surface of the needle handle is 5 ~ 7 mm, the long axis of the lower elliptical surface is 4 ~ 6 mm, the short axis of the lower elliptical surface is 5 ~ 7 mm, the length of the needle handle in the vertical direction is 18 ~ 20 mm, a rectangular plane is arranged on the elliptical cylindrical surface of the needle handle, the length of the rectangular plane is 8 ~ 10 mm, the width of the rectangular plane is 3 ~ 2 mm, and the rectangular plane is provided with a straight line parallel to the whole puncture needle.
A straight line parallel to the puncture needle is arranged on the rectangular plane of the needle handle and is parallel to the auxiliary straight line on the butt joint fixer.
The far end fixing puncture part of the L-shaped steel butt joint fixer forms an angle of 110 ~ 120 degrees with the near end rod part, the far end fixing puncture part is provided with a water-pouring-drop-shaped needle hole, and the needle hole is matched with a water-pouring-drop-shaped insulator of the puncture needle close to the needle point.
The thickness of the butt joint fixer is 0.1 ~ 0.4.4 mm, the length of the proximal rod part is 30 ~ 50 mm, the thickness is 0.5 ~ 1 mm, the width is 2 ~ 4 mm, the length of the distal fixing puncture part is 4 ~ 5 mm, and the outer edge of the distal fixing puncture part 7 is provided with a blade.
The inner side of the pinhole is coated with an insulator substance; a plurality of pointing grooves are arranged around the needle hole from outside to inside in the circumferential direction from shallow to deep.
An auxiliary straight line parallel to the straight line on the needle handle is arranged on the rod part of the near end of the butt joint fixer.
The advantages and effects are as follows:
the utility model has the advantages of it is following and beneficial effect:
the action potential of the myocardial cells is the basis for researching various pathogenic factors causing the change of the electrocardiogram, and the acquisition device of the utility model acquires the action potential of the myocardial cells under the condition that the thoracic cavity of a rat is not opened. The rat can survive for a long time, and the puncture needle can be rapidly positioned in the thoracic cavity of the rat by matching with the butt joint fixer.
Meanwhile, the puncture needle is matched with the butt joint fixator for use, so that ventricular cell action potentials can be easily collected, and a lot of experimental animals can be saved.
The cooperation the utility model discloses a collection system need not open the rat thorax, and the collection process is rapid, and the collection position is accurate.
Description of the drawings:
fig. 1 is a structural schematic diagram of the puncture needle.
Fig. 2 is a schematic view of the docking fixture configuration.
Fig. 3 is a schematic diagram of the practical use of the collecting device of the present invention.
Fig. 4 is a schematic view of the structure of the needle shaft of the puncture needle.
FIG. 5 is a schematic diagram of the insertion of a pin hole and an inverted drop insulator.
Description of reference numerals:
1. puncturing needle; 2. a butt-joint fixer; 3. a needle tip; 4. an inverted drop-shaped insulator; 5. a needle handle; 6. a wire; 7. the puncture part is fixed at the far end; 8. a proximal stem portion; 9. a pinhole; 10. pointing to the grooves; 11. an auxiliary straight line.
The specific implementation mode is as follows:
the invention will be further explained with reference to the drawings:
as shown in fig. 1, fig. 2 and fig. 3, the experimental rat myocardial cell action potential acquisition device comprises a puncture needle 1 and a docking fixer 2, wherein the puncture needle 1 is inserted into the docking fixer 2; the insertion means that the needle point 3 of the puncture needle 1 is inserted into the thoracic cavity of a rat through the needle hole 9 of the butt joint fixer 2, the depth of the needle point 3 entering the cardiac muscle is limited through the water drop-shaped needle hole 9 and the inverted water drop-shaped insulator 4 matched with the shape of the water drop-shaped needle hole 9, and the needle point 3 is prevented from excessively penetrating into a heart wall chamber.
The acquisition device of the utility model acquires the action potential of the rat myocardial cells by matching the puncture needle 1 and the butt joint fixer 2; the action potential of the myocardial cells can be collected under the condition of not opening the thoracic cavity of the rat.
As shown in figure 1, the lower end of a puncture needle 1 which is integrally made of steel is provided with a needle point 3, an inverted water drop-shaped insulator 4 is fixed above the needle point 3, a needle handle 5 is arranged on the puncture needle 1, the upper end of the puncture needle 1 is provided with a lead 6 connected with an electrocardiosignal collector, the tip at the lowest end of the puncture needle 1 is the needle point 3, the needle point 3 is used for penetrating the epidermis of a rat and collecting action potentials of myocardial cells, the inverted water drop-shaped insulator 4 above the needle point 3 is made of hard materials and is not easy to deform, the puncture needle 1 is ensured to be inserted on a butt-joint fixer 2 and cannot be inserted too deeply, a through hole is reserved in the middle of the needle handle 5, the needle handle 5 is fixed on the needle body of the puncture needle 1 through the through hole, the needle handle 5 is arranged at the middle rear end of the puncture needle 1 and is generally 3 ~ 5 mm away from the highest end.
The needle point 3 of the puncture needle 1 and the puncture needle 1 are of an integral structure, the inverted water drop-shaped insulator 4 is fixed on the puncture needle 1, the needle handle 5 is fixed at the upper end of the puncture needle 1, and an insulating layer is arranged outside the needle body between the inverted water drop-shaped insulator 4 and the needle handle 5 to prevent electric signals mixed in other parts of the rat body. As can be seen from figure 2, a certain distance is left between the connection part of the puncture needle and the uppermost wire 6 of the puncture needle 1, the part which is not coated with the insulating layer above the puncture needle 1 is connected with the wire 6, the connection mode can be welding, intersection connection or other connection modes, and the wire 6 is connected with the electrocardiosignal collector.
As shown in figure 1, the puncture needle 1 has the whole needle length of 30 ~ 40 mm, the needle tip 3 has the length of 0.3 ~ 0.5.5 mm, the inverted water drop-shaped insulator 4 above the needle tip 3 has the short diameter of 0.5 ~ 1.0.0 mm, the long diameter of 2.0 ~ 3.0.0 mm and the length in the vertical direction of 2.5 ~ 4.0.0 mm, the inverted water drop-shaped insulator 4 can facilitate the puncture needle 1 to enter the thoracic cavity, and simultaneously prevent the needle tip 3 from excessively penetrating into the ventricular wall after being connected with the puncture hole, the surface above the needle tip 3 of the puncture needle 1 is covered with the insulator, the needle tip 3 is not covered with the insulator, and the situation that other parts of the puncture needle 1 are conductive, the lead 6 connected with the upper end of the puncture needle 1 is influenced, and the myocardial action potential collected by an electrocardiosignal collector is.
As shown in fig. 4, the needle shaft 5 has an oval horizontal surface, a trapezoidal coronal surface and a trapezoidal sagittal surface. The needle handle 5 is an insulator, the needle core of the puncture needle 1 is a conductor, the puncture needle 1 is provided with an inverted water drop-shaped insulator 4, a needle body part of the puncture needle 1 and the needle handle 5 in sequence from the needle point 3, and then is connected with an electrocardiosignal collector through a lead 6.
As shown in fig. 4, the major axis of the elliptic surface of the elliptic cylindrical needle handle 5 is 8 ~ 12 mm, the minor axis thereof is 5 ~ 7 mm, the major axis thereof is 4 ~ 6 mm, the minor axis thereof is 5 ~ 7 mm, the vertical length of the needle handle 5 is 18 ~ 20 mm, the elliptic cylindrical surface of the needle handle 5 has a rectangular plane, the length of the rectangular plane is 8 ~ 10 mm, the width thereof is 3 ~ 2 mm, the rectangular plane has a straight line parallel to the whole puncture needle, and the rectangular plane is a rectangular plane cut out from the elliptic cylindrical surface of the needle handle 5, mainly in order to arrange a straight line parallel to the needle body of the puncture needle 1.
A straight line parallel to the puncture needle 1 is arranged on a rectangular plane of the needle handle 5, the straight line can be directly marked on the puncture needle 1 or can be drawn through colored pigments, similarly, the auxiliary straight line 11 on the butt joint fixer 2 is also arranged by the same method, the straight line on the rectangular plane is parallel to the auxiliary straight line 11 on the butt joint fixer 2, the direction of the puncture needle 1 can be indicated, and the needle tip 3 is inserted into a needle hole 9 of a far-end fixed puncture part 7 of the butt joint fixer 2 and then is parallel to a near-end rod part 8 of the butt joint fixer 2.
The included angle between the far-end fixed puncture part 7 and the near-end rod part 8 of the L-shaped steel butt-joint fixer 2 is 110 ~ 120 degrees, the far-end fixed puncture part 7 is provided with a reversed water droplet sharp pinhole 9, the pinhole 9 is matched with the reversed water droplet-shaped insulator 4 of the puncture needle 1 close to the needle point 3, as shown in fig. 5, the matching means that the water droplet sharp at the front end of the reversed water droplet-shaped insulator 4 is matched with the size of the pinhole 9, the shape matching is maintained, meanwhile, the needle point part is not inserted too deeply, namely, the deep distance of the needle point is limited, the situation that the needle point penetrates too deeply into the heart to cause death of a rat is prevented, the reversed water droplet sharp pinhole 9 is a hole formed by penetrating the far-end fixed puncture part 7, and the distance between the pinhole 9 and the outer end of the far-end fixed puncture part 7 is 1.2. 1.2 ~ 2.0 mm.
The thickness of the butt fixator 2 is 0.1 ~ 0.4.4 mm, the length of the proximal rod part 8 is 30 ~ 50 mm, the thickness is 0.5 ~ 1 mm, the tip of the distal fixing puncture part 7 is thinner, the width is 2 ~ 4 mm, the length of the distal fixing puncture part 7 is 4 ~ 5 mm, the outer edge of the distal fixing puncture part 7 is provided with a blade, a reversed water-drop-point-shaped pinhole 9 is arranged near one third of the outer side of the distal fixing puncture part 7, the size of the reversed water-drop-point-shaped pinhole 9 is matched with the reversed water-drop-shaped insulator 4 of the puncture needle 1, and the puncture needle 1 can swing towards different directions.
The inner side of the pinhole 9 is coated with an insulating material, a plurality of pointing grooves 10 are arranged around the pinhole 9 from outside to inside from shallow to deep, the inner side of the pinhole 9 and the area of 1 ~ 2 mm around the pinhole 9 are all covered with a hard insulating material to prevent electrocardiosignals on the body surface of a rat from mixing and interfering, a plurality of pointing grooves 10 are uniformly arranged around the pinhole 9 by taking the pinhole 9 as the center of a circle, the pointing grooves 10 are from deep to shallow from the outside of the pinhole 9, the position of the pinhole 9 can be conveniently confirmed by an operator without visual observation, as shown in figure 2, the number of the pointing grooves 10 can be 4, 6, 8 and the like, and the insulating material can be the existing inorganic insulating paint, or can be rubber, silica gel and the like coated on the inner side of the pinhole 9 and the area around the pinhole 9.
An auxiliary straight line 11 parallel to the straight line on the needle handle 5 is arranged on the rod part 8 at the near end of the butt joint fixer 2. The auxiliary straight line 11 is parallel to the straight line on the needle handle 5 of the puncture needle 1 when the cardiac cell action potential is collected.
As shown in fig. 3, a method for collecting myocardial cell action potential of an experimental rat, the method specifically includes:
the method comprises the following steps: after the rat is anesthetized, the puncture needle 1 is punctured into the thoracic cavity of the rat along the puncture point, and the butt joint fixer 2 is punctured into the thoracic cavity from the next intercostal;
step two: the straight line on the rectangular plane at the front end of the sagittal plane of the needle handle 5 of the puncture needle 1 and the auxiliary straight line 11 on the butt joint fixer 2 are in the same plane;
step three: the needle point 3 of the puncture needle 1 is contacted with the far-end fixed puncture part 7 of the butt joint fixer 2, slides into the needle hole 9 along the upper directional groove 10, and moves to the heart of the experimental rat together, the needle point 3 punctures into the chamber wall of the heart, and the held finger end has wave motion; the lead 6 at the upper end of the puncture needle 1 is connected with an electrocardiosignal collector, so that action potential signals of ventricular muscles can be collected.
The collecting method has the advantages that the collecting method does not need to open the chest cavity of the rat for collecting, and the condition that the heart of the rat stops beating due to overlong operation time, so that action potentials of myocardial cells cannot be collected is prevented.
Claims (9)
1. Experiment rat cardiac muscle cell action potential collection system, its characterized in that: the collecting device comprises a puncture needle (1) and a butt joint fixer (2), and the puncture needle (1) is inserted into the butt joint fixer (2).
2. The device for acquiring action potential of myocardial cell of experimental rat according to claim 1, wherein: the lower end of the puncture needle (1) which is integrally made of steel is provided with a needle point (3), a reversed water drop-shaped insulator (4) is fixed above the needle point (3), and a needle handle (5) is arranged on the puncture needle (1); the upper end of the puncture needle (1) is provided with a lead (6) connected with an electrocardiosignal collector.
3. The experimental rat myocardial cell action potential collecting device according to claim 2, characterized in that the puncture needle (1) has a needle length of 30 ~ 40 mm, the needle tip (3) has a needle length of 0.3 ~ 0.5.5 mm, the inverted drop-shaped insulator (4) above the needle tip (3) has a short diameter of 0.5 ~ 1.0.0 mm, a long diameter of 2.0 ~ 3.0.0 mm and a vertical length of 2.5 ~ 4.0.0 mm, and the needle handle (5) has an oval horizontal surface, a trapezoidal crown surface and a trapezoidal sagittal surface.
4. The experimental rat myocardial cell action potential collecting device according to claim 3, characterized in that the elliptic cylindrical needle handle (5) has a major axis of 8 ~ 12 mm and a minor axis of 5 ~ 7 mm, a major axis of 4 ~ 6 mm and a minor axis of 5 ~ 7 mm, the needle handle (5) has a vertical length of 18 ~ 20 mm, the elliptic cylindrical surface of the needle handle (5) has a rectangular plane, the length of the rectangular plane is 8 ~ 10 mm and the width of the rectangular plane is 3 ~ 2 mm, and the rectangular plane has a straight line parallel to the whole puncture needle (1).
5. The device for acquiring action potential of myocardial cell of experimental rat according to claim 4, wherein: a straight line parallel to the puncture needle (1) is arranged on a rectangular plane of the needle handle (5), the straight line is parallel to an auxiliary straight line (11) on the butt joint fixer (2), the direction of the puncture needle (1) can be indicated, and the needle point (3) is inserted into a needle hole (9) of a far-end fixed puncture part (7) of the butt joint fixer (2) and then is parallel to a near-end rod part (8) of the butt joint fixer (2).
6. The experimental rat myocardial cell action potential acquisition device according to claim 1, characterized in that the far end fixed puncture part (7) of the L-shaped steel butt-joint fixer (2) and the near end rod part (8) form an angle of 110 ~ 120 degrees, the far end fixed puncture part (7) is provided with a reversed water drop-shaped needle hole (9), and the needle hole (9) is matched with a reversed water drop-shaped insulator (4) of the puncture needle (1) close to the needle point (3).
7. The experimental rat myocardial cell action potential collecting device according to claim 6, characterized in that the docking fixator (2) has a thickness of 0.1 ~ 0.4.4 mm, a proximal rod portion (8) has a length of 30 ~ 50 mm, a thickness of 0.5 ~ 1 mm, a width of 2 ~ 4 mm, a distal fixed puncture portion (7) has a length of 4 ~ 5 mm, and the outer edge of the distal fixed puncture portion (7) is provided with a blade.
8. The device for acquiring action potential of myocardial cell of experimental rat according to claim 6, wherein: the inner side of the pinhole (9) is coated with an insulator substance; a plurality of pointing grooves (10) from shallow to deep are arranged around the pinhole (9) from outside to inside in the circumferential direction.
9. The device for acquiring action potential of myocardial cell of experimental rat according to claim 6 or 7, wherein: an auxiliary straight line (11) parallel to the straight line on the needle handle (5) is arranged on the rod part (8) at the near end of the butt joint fixer (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920020552.7U CN209826716U (en) | 2019-01-07 | 2019-01-07 | Experimental rat myocardial cell action potential acquisition device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920020552.7U CN209826716U (en) | 2019-01-07 | 2019-01-07 | Experimental rat myocardial cell action potential acquisition device |
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| CN209826716U true CN209826716U (en) | 2019-12-24 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109645982A (en) * | 2019-01-07 | 2019-04-19 | 沈阳工业大学 | Experimental rat Single Cardiac Cell acquisition device |
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2019
- 2019-01-07 CN CN201920020552.7U patent/CN209826716U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109645982A (en) * | 2019-01-07 | 2019-04-19 | 沈阳工业大学 | Experimental rat Single Cardiac Cell acquisition device |
| CN109645982B (en) * | 2019-01-07 | 2024-04-30 | 沈阳工业大学 | Experimental rat myocardial cell action potential acquisition device |
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| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191224 Termination date: 20220107 |