CN209764704U - photoacoustic detection experimental device for adolescent rat spermatic varicocele model - Google Patents
photoacoustic detection experimental device for adolescent rat spermatic varicocele model Download PDFInfo
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- CN209764704U CN209764704U CN201920280241.4U CN201920280241U CN209764704U CN 209764704 U CN209764704 U CN 209764704U CN 201920280241 U CN201920280241 U CN 201920280241U CN 209764704 U CN209764704 U CN 209764704U
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Abstract
The utility model discloses an optoacoustic detection experimental device of a rat varicocele model in adolescence, which comprises a water container, wherein a rat testis is placed in water; the laser emitter emits laser beams to irradiate the testis of the rat; a needle-shaped hydrophone rotating around the rat testis to obtain a photoacoustic signal; the driver provides power for the needle-shaped hydrophone to rotate; and the computer controls the laser transmitter, receives and calculates the photoacoustic signals obtained by the needle-shaped hydrophones so as to reconstruct a blood vessel network image. The utility model discloses can carry out the detection experiment of rat spermatic cord varicosity through optoacoustic imaging to supply teaching and verification.
Description
Technical Field
The utility model belongs to optoacoustic detection experiment technique, concretely relates to optoacoustic detection experimental apparatus of adolescent rat varicocele model of spermocele's varicocele.
background
Varicocele (VC) refers to the stretching, dilation, and tortuosity of the tendrilous venous plexus due to factors such as blocked venous return or valve failure and blood reflux. In recent 20 years of clinical research, the varicocele is one of the most important causes of male infertility, the incidence rate of the patient is 10-15%, and in about 40% of adult males suffering from the primary infertility and 80% of adult males suffering from the varicocele, the fertility of 20-50% of patients is restored through surgical operation and other treatment means. However, the current auxiliary diagnosis mode cannot reflect the state of an illness in time, a more timely and noninvasive detection method is found, and the method has important significance for judging the operation time of varicocele.
Tissue hypoxia caused by venous blood stasis is an important expression of varicocele, and the photoacoustic detection applied to the medical field in recent years has unique advantages on the change of tissue blood oxygen, so that the method is presumed as follows: the photoacoustic detection can timely and effectively evaluate the state of illness of varicocele.
the basic principle of medical optoacoustic is as follows: the pulse laser with different wavelengths irradiates the biological tissue and is absorbed to generate transient micro temperature rise, and the biological tissue releases ultrasonic signals, namely photoacoustic signals, due to the thermoelastic effect; the signals carry abundant information of optical, elastic, thermodynamic and structural of tissues and can be detected by an ultrasonic transducer with high sensitivity; the received photoacoustic signals can be used to reconstruct images of biological tissue for disease diagnosis and tissue assessment:
The method comprises the steps of firstly, displaying the shapes of a testicular vascular network and an epididymal duct with high resolution by using photoacoustic imaging, and secondly, further measuring the blood oxygen metabolism in the testicular vascular network by respectively observing aerobic and deoxygenated red blood cells by using the photoacoustic imaging in functional imaging. The photoacoustic can simultaneously detect parameters of the cross section area of the blood vessel, the concentration of hemoglobin, the oxidation level of hemoglobin and the blood flow velocity, so the photoacoustic has the advantage of reflecting the metabolism level of the testis, which is not available in other technologies. Moreover, the photoacoustic detection is convenient, low in price and free of radiation, and is completely suitable for timely evaluating the state of the varicocele in theory and application.
Therefore, by establishing a pubertal rat varicocele model and verifying the feasibility and the effect of photoacoustic detection by using the animal model, a research and development basis is provided for establishing an evaluation system of high-resolution functional imaging of juvenile varicocele in the future, and the method is a problem which needs to be solved urgently at present.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a detection experiment of rat spermatic varicocele can be carried out through optoacoustic imaging to the optoacoustic detection experimental apparatus of the rat spermatic varicocele model of puberty is provided.
In order to solve the technical problem, the utility model discloses a following technical scheme:
An experimental apparatus for photoacoustic detection of adolescent rat varicocele model comprises:
A water container which contains water for placing the tested rat testis;
The laser emitter emits laser beams to irradiate the testis of the rat;
The needle-shaped hydrophone is placed in water and horizontally rotates around a rat testis to obtain a photoacoustic signal;
the driver is connected with the needle-shaped hydrophone by adopting a stepping motor and provides power for the needle-shaped hydrophone to rotate;
and the computer controls the laser transmitter, receives and calculates the photoacoustic signals obtained by the needle-shaped hydrophones so as to reconstruct a blood vessel network image.
The laser triggering device also comprises a signal amplifier and a digital oscilloscope, wherein the input end of the signal amplifier is connected with the needle-shaped hydrophone, the output end of the signal amplifier is connected with the digital oscilloscope, and the digital oscilloscope simultaneously receives the photoacoustic signal amplified by the signal amplifier and the laser triggering signal of the laser emitter and outputs the photoacoustic signal and the laser triggering signal to the computer.
the rated bandwidth of the hydrophone is 0.1-10 MHz.
Adopt the utility model discloses a light acoustic detection experimental apparatus of adolescent rat spermatic varicosity model has following advantage:
1. The detection experiment of rat varicocele can be realized through photoacoustic imaging for teaching and research (non-disease diagnosis and treatment purpose);
2. The method is beneficial to the verification and research (non-disease diagnosis and treatment purposes) of the photoacoustic imaging technology of the varicocele of teenagers;
3. According to the volume and the blood vessel distribution of the testis of the rat, the photoacoustic detection system capable of rotating around the testis of the rat is designed, and the shape and the thickness of the spermatic cord blood vessel and the spermatic cord blood vessel network can be displayed in a non-invasive and non-radiative manner, so that the change of the spermatic cord blood vessel and the spermatic cord blood vessel network of the rat can be dynamically observed.
drawings
Fig. 1 is a schematic diagram of the photoacoustic detection experimental apparatus of the present invention.
Detailed Description
The utility model discloses a photo-acoustic detection experimental device of a rat spermatic varicocele model in adolescence, which is shown in figure 1 and mainly comprises a water container 1, such as a water jar and the like, wherein water is contained in the water container and is used for placing a tested rat testis 2 in the water; a laser emitter 3 for emitting a laser beam of 532nm to irradiate the rat testis 2 placed in the water container 1; a needle-shaped hydrophone 4 with the rated bandwidth of 0.1-10MHz is horizontally arranged in the water of the water container 1 and horizontally rotates around a rat testis 2 to obtain a photoacoustic signal; the driver 5 can adopt a stepping motor, can be connected with the needle-shaped hydrophone 4 in a connecting mode such as a connecting bracket 6 and the like, and provides power for the horizontal revolution of the needle-shaped hydrophone 4; and the computer 7 controls the light energy density and the like of the laser transmitter 3, simultaneously receives the photoacoustic signals obtained by the needle-shaped hydrophones 4 and carries out corresponding calculation so as to reconstruct a blood vessel network image. The detection experimental device also comprises a signal amplifier 8 and a digital oscilloscope 9, wherein the input end of the signal amplifier 8 is connected with the needle-shaped hydrophone 4, and the output end of the signal amplifier 8 is connected with the digital oscilloscope 9 and is used for amplifying the received photoacoustic signals. And the digital oscilloscope 9 has a sampling rate of 250MHz, has an input end connected with the signal amplifier 8 for acquisition and display, has an output end connected with the computer 7, and outputs the photoacoustic signal amplified by the signal amplifier 8 and the laser trigger signal of the laser emitter 3 to the computer 7.
Adopt the utility model discloses a detection experiment device's experimental method as follows:
A. Killing the mouse, taking out the testis 2, embedding and fixing the testis 2 by gel, and placing the testis in a water container 1;
B. a laser emitter 3 is used for emitting laser beams, the testis 2 in the water container 1 is irradiated by the laser beams, and the laser beams are adjusted to completely cover the testis; the laser transmitter 3 emits 532nm laser beams, and the light energy density is controlled to be 6mJ/cm2；
C. Driving a hydrophone to horizontally rotate around a testis through a stepping motor so as to acquire a photoacoustic signal;
D. after the obtained photoacoustic signal is amplified to 40dB by a signal amplifier 8, a digital oscilloscope 9 with the sampling rate of 250MHz is used for acquisition and display;
E. Adopt computer 7 will use the laser energy who records in the experiment to carry out the normalization to the photoacoustic signal intensity of gathering to calculate the result in Matlab software (this calculating method is not the innovation point of the utility model, no longer describe here) with rebuild the blood vessel net image.
however, those skilled in the art should realize that the above embodiments are only used for illustrating the present invention and not used as a limitation of the present invention, and that the changes and modifications to the above embodiments are within the scope of the appended claims as long as they are within the true spirit of the present invention.
Claims (3)
1. An experimental apparatus for photoacoustic detection of adolescent rat varicocele model is characterized by comprising:
A water container which contains water for placing the tested rat testis;
The laser emitter emits laser beams to irradiate the testis of the rat;
the needle-shaped hydrophone is placed in water and horizontally rotates around a rat testis to obtain a photoacoustic signal;
The driver is connected with the needle-shaped hydrophone by adopting a stepping motor and provides power for the needle-shaped hydrophone to rotate;
And the computer controls the laser transmitter, receives and calculates the photoacoustic signals obtained by the needle-shaped hydrophones so as to reconstruct a blood vessel network image.
2. The experimental apparatus for photoacoustic detection of varicocele model in adolescent rat according to claim 1, further comprising a signal amplifier and a digital oscilloscope, wherein the input end of the signal amplifier is connected to the needle-shaped hydrophone, and the output end of the signal amplifier is connected to the digital oscilloscope, and the digital oscilloscope receives the photoacoustic signal amplified by the signal amplifier and the laser trigger signal of the laser emitter at the same time and outputs the photoacoustic signal and the laser trigger signal to the computer.
3. The experimental apparatus for photoacoustic detection of the adolescent rat varicocele model according to claim 1, wherein the nominal bandwidth of the hydrophone is 0.1-10 MHz.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109870413A (en) * | 2019-03-05 | 2019-06-11 | 上海市同济医院 | The photo-acoustic detection experimental provision and method of Adolescent Rats varicocele model |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109870413A (en) * | 2019-03-05 | 2019-06-11 | 上海市同济医院 | The photo-acoustic detection experimental provision and method of Adolescent Rats varicocele model |
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