CN209685786U - A kind of culture apparatus of Anaerobic culturel - Google Patents

Abstract

The utility model relates to biological bacterium culture technique field more particularly to a kind of culture apparatus of Anaerobic culturel, including ware body and ware lid, ware lid is threadedly coupled with the ware body, filled chamber with an open mouth is provided in ware lid, and filled chamber recess is set in ware lid；Filled chamber is provided with the hermatic door for blocking the upper opening, and hermatic door is concordant with the cover board of ware lid；Hermatic door and the filled chamber are rotatablely connected, the bottom of filled chamber offers several through-holes, the strip concave block of anaerobism bar is placed in setting one on hermatic door inner wall, the opening of strip concave block is towards the bottom of filled chamber, it surrounds and is opened up on the side wall of strip concave block opening there are two card slot, two card slots are oppositely arranged along the length direction of strip concave block.The advantages of the utility model: ingenious in design, the amount for the anaerobism agent that more preferable, easy to operate, independent, Anaerobic culturel process uses with sealing performance is reduced, and is improved Anaerobic culturel effect, efficiency to reach, is reduced Anaerobic culturel cost.

Description

A kind of culture apparatus of Anaerobic culturel

Technical field

The utility model relates to biological bacterium culture technique field more particularly to a kind of culture apparatus of Anaerobic culturel.

Background technique

(mainly there is clostridium botulinum, C.perfringens, clostridium tetani, difficult in the microbial bacteria for doing anaerobic gas generation at present Difficult clostridium etc.) culture when, adoptable method has: the methods of anaerobism cylinder method, airbag method, anaerobism glove box, for existing Common airbag method, generally use transparent plastic bag, built-in gas generating tube (have sodium borohydride sodium bicarbonate solid and 5% citric acid ampoule), methylene blue indicator pipe, palladium catalyst pipe, desiccant.After being put into inoculated plate, bag is squeezed out as far as possible Interior air, then seals sack.First fracture gas generating tube, after fracture methylene blue indicator pipe, nothing is caused within half an hour in bag Oxygen environment.But airbag also has certain spatial dimension, and anaerobism agent market price is expensive, and plate is placed an airbag and just needed using falling one Packet anaerobism agent ensures anaerobic culture environment.If using filling CO₂Method carry out, need additionally be arranged be equipped with individually The container conduit of carbon dioxide, and pure CO is reported without correlative study₂Condition is suitable for the growth of anaerobic bacteria.In addition it many times needs It once to carry out the plate of several repetitions and/or several gradients or the culture plate of several different specimens while cultivate, if in Way takes out one of culture plate and checks cultivation results, other culture plates will touch oxygen, to other culture plates Culture environment impact.

Therefore, design it is a kind of with good seal performance, independent, Anaerobic culturel process using anaerobism agent amount it is few, thus Anaerobic culturel effect and efficiency are improved, and the culture apparatus for reducing Anaerobic culturel cost is particularly important.

Summary of the invention

In view of above content, it is necessary to a kind of culture apparatus of Anaerobic culturel is provided, it is more preferable with sealing performance, independent , easy to operate, Anaerobic culturel process reduced using the amount of anaerobism agent, to improve Anaerobic culturel effect and efficiency, and reduce Anaerobic culturel cost.

In order to achieve the above object, the technical scheme adopted by the utility model is

A kind of culture apparatus of Anaerobic culturel, including ware body and ware lid, the ware lid is threadedly coupled with the ware body, described Filled chamber with an open mouth is provided in ware lid, and filled chamber recess is set in ware lid；The filled chamber is provided with For blocking the hermatic door of the upper opening, the hermatic door is concordant with the capping of ware lid；The hermatic door and the filled chamber Rotation connection, the bottom of the filled chamber offer several through-holes, and anaerobism bar is placed in setting one on the hermatic door inner wall Strip concave block, the opening of the strip concave block is towards the bottom of filled chamber, on the side wall for surrounding strip concave block opening It opens up there are two card slot, two card slots are oppositely arranged along the length direction of the strip concave block.

Further, the hermatic door is rotatablely connected by rotation axis and the filled chamber, and torsion is socketed in rotation axis Turn torsional spring, and torsionspring one end connects hermatic door, other end connects filled chamber, so that hermatic door is resilient with filled chamber Folding condition.

Further, the upper limb of the upper opening is fixed with rubber ring, and the edge of the hermatic door is also fixed with corresponding Rubber ring.

Further, also settable 1-3 millimeters of capping lower than ware lid of the hermatic door.

Further, the flush type handle for pulling the hermatic door is provided on the hermatic door.

Further, the material of the hermatic door, strip concave block and filled chamber is thermostable transparent plastic material system At.

Further, the material of the ware lid is made of thermostable transparent plastics or transparent glass material.

It further illustrates, rubber ring involved in the application is that high temperature resistant material is made.

Further, the height of the filled chamber is the 1/3-1/2 of ware lid height.

Due to the adoption of the above technical scheme, the utility model has the following beneficial effects:

1, better seal can be played with ware body threaded connection using ware lid to connect, isolation air enters culture dish It is interior, it is provided with the filled chamber for placing anaerobism agent in ware lid, opens hermatic door when needing to place anaerobism agent in filled chamber, Then hermatic door is closed, the bottom of filled chamber offers several through-holes, the space in the culture apparatus of through-hole and Anaerobic culturel Connection, can absorb the oxygen in space, can continuous adsorption of oxygen during Anaerobic culturel；By anaerobism bar along in ware lid Along the card slot insertion of card slot and the lower edge setting of setting on groove, can be fixed in ware lid, for checking the training of Anaerobic culturel Support the anaerobic environment situation in device.

2, hermatic door and filled chamber are rotatablely connected by torque spring, the switch that hermatic door can be more convenient.

3, the upper limb of upper opening is fixed with rubber ring, and the edge of hermatic door is also fixed with corresponding rubber ring, can guarantee Filled chamber better tightness when sealing under door closure state.

4, ware lid, filled chamber, hermatic door are all made of transparent material, in the culture apparatus for facilitating observation Anaerobic culturel The case where.

5, the smart structural design of the application, device can independently, there is better sealing, and exempted from set anaerobism bag, squeezed Air, then sack is sealed, so that Anaerobic culturel is easy to operate.And Anaerobic culturel airbag method, which is inconvenient to gather into folds, puts It sets, irregular also to take up space very much, using the design structure of the application, plate is independent, can stack while cultivate, save culture Space.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of the culture apparatus disassembled form of 1 Anaerobic culturel of embodiment；

Fig. 2 is the structural schematic diagram of concave block；

Fig. 3 is the structural schematic diagram of filled chamber and hermatic door opening state.

In figure, 1- hermatic door, 2- flush type handle, 3- ware lid, 31- cover board, 32- cover sidewall, 4- external screw thread, 5- ware Body, 6- internal screw thread, 7- card slot, 8- through-hole, 9- strip concave block, 10- filled chamber, the bottom of 11- filled chamber, 12- rubber ring, 13- shaft, 14- torsionspring.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.

It should be noted that it can be directly on another component when component is referred to as " being fixed on " another component Or there may also be components placed in the middle.When a component is considered as " connection " another component, it, which can be, is directly connected to To another component or it may be simultaneously present component placed in the middle.When a component is considered as " being set to " another component, it It can be and be set up directly on another component or may be simultaneously present component placed in the middle.Term as used herein is " vertical ", " horizontal ", "left", "right" and similar statement for illustrative purposes only.

Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term " and or " used herein includes Any and all combinations of one or more related listed items.

Referring to Figure 1 to Fig. 3, one better embodiment of the utility model provides a kind of culture apparatus of Anaerobic culturel, packet Ware body 5 and ware lid 3 are included, the ware lid 3 is threadedly coupled with the ware body 5,

Ware body 5 includes a bottom plate and surround the side wall for being connected to base portion peripheral, and the middle and upper part of ware body 5 is provided with external screw thread 4 (being as provided with external screw thread 4 on the outer wall of the middle and upper part of side wall)；

Ware lid 3 is including a cover board 31 and around the cover sidewall 32 for being connected to 31 periphery of cover board, the peripheral wall of filled chamber 10 It is fixedly connected with cover board 31；

(opening and separate cover board 31 close to ware lid 3) is correspondingly arranged on and ware body 5 on the inner wall of 3 cover sidewall 32 of ware lid The internal screw thread 6 that the external screw thread 4 of upper setting is adapted to；

External screw thread 4 and internal screw thread 6 are all made of existing technique processing and fabricating；

Filled chamber 10 with an open mouth is provided on 3 inner wall of ware lid, and the recess of the filled chamber 10 is set to ware In lid 3；As ware lid 3 includes a cover board 31 and around being connected to the cover sidewall 32 of 31 periphery of cover board, cover board 31 and is surround Be connected to 31 periphery of cover board cover sidewall 32 formed it is interior it is intracavitary be fixed with one have opening filled chamber 10, filled chamber 10 Peripheral wall apical position is fixedly connected with cover board 31, and the opening of filled chamber 10 is close to cover board 31, and the bottom of filled chamber 10 is far from cover board 31；The shape of the opening of the corresponding filled chamber 10 of cover board 31 is correspondingly arranged on opening, the 1 Corresponding matching filled chamber of hermatic door being arranged afterwards 10 opening setting；The shape of filled chamber 10 can be cuboid and be also possible to cylindric or square；

The filled chamber 10 is provided with the hermatic door 1 for blocking the upper opening, the lid of the hermatic door 1 and ware lid 3 Plate 31 is concordant；The area of upper opening is the 1/3-1/2 of 31 area of cover board of ware lid 3；Filled chamber 10 is preferably fixed on ware lid 3 The middle position of cover board 31；The height of preferred filled chamber 10 is the 1/3-1/2 of 3 height of ware lid.

Hermatic door 1 is made of transparent plastic, and the top surface of hermatic door 1 wants low with the cover board 31 of ware lid 3, in this way can be To culture apparatus in the case where being buckled to culture, the problem of will not influence placement.

Hermatic door 1 is rotatablely connected by rotation axis and the filled chamber 10, and torsion torsional spring is socketed in rotation axis, and 14 one end of torsionspring connects hermatic door 1, and other end connects filled chamber 10, so that hermatic door 1 is resilient out with filled chamber 10 Conjunction state.

The bottom 11 of filled chamber offers several through-holes 8, through-hole 8 be able to achieve filled chamber 10 be threadedly coupled after the training that is formed The inner cavity (as in the space of culture anaerobe) that device is formed is supported, after placing anaerobism agent in filled chamber 10, can be inhaled The oxygen in space is received, anaerobic environment is manufactured.

The strip concave block 9 of anaerobism bar, strip concave block 9 and sealing are placed in setting one on 1 inner wall of hermatic door 1 inner wall of door is fixedly connected, and the opening of the strip concave block 9 surrounds the opening of strip concave block 9 towards the bottom of filled chamber 1 It is opened up on side wall there are two card slot 7, two card slots 7 are oppositely arranged along the length direction of the strip concave block 9.It can be by anaerobism Bar is inserted into along the length direction of card slot 7, and anaerobism bar is strip, and the width of anaerobism bar can be just adapted to Opened up on the two sidewalls that strip concave block 9 is open it is the distance between opposite there are two card slot 7 so that anaerobism bar is fixed on length In bar shaped concave block 9, convenient replacement anaerobism bar every time, the effect of anaerobism bar is the culture apparatus that can indicate Anaerobic culturel Interior oxygen-free environment situation.

The upper limb of upper opening is fixed with rubber ring 12, and the edge of hermatic door 1 is also fixed with corresponding rubber ring 12, two rubber The effect of circle 12 is to can be improved the leakproofness of filled chamber 10 in the case where hermatic door 1 and filled chamber 10 are in lid conjunction state.Rubber The fixation of circle 12 is fixed using the processing technology processing of the prior art.

Also settable 1-3 millimeters of capping lower than ware lid 3 of hermatic door 1.

The side of ware lid 3 and the ware body 5 is provided with handle.It is convenient more convenient in torsion, Fast Installation and It splits.This Technology design can be used as other preferred embodiment, in figure and be not drawn into.

The handle for pulling the hermatic door 1 is provided on hermatic door 1.The handle is flush type handle 2.

Hermatic door 1, strip concave block 9, ware lid 3, flush type handle 2, filled chamber 10 material be transparent plastics material It is made.It is made of transparent plastics material, to facilitate the state for cultivating bacterium in observation culture apparatus, observation anaerobism bar is shown Show state to determine the anaerobic conditions in culture apparatus.Transparent plastics material is thermostable transparent plastic material；High temperature resistant is saturating Bright material is processed into using prior art and formula.

Work step:

1. culture apparatus is cleaned, it is put into baking oven and is dried for standby；

2. culture apparatus is combined by a ware lid and a ware body spiral a set of, a set of culture can will be formed with newspaper and filled It sets and is bundled into a packet；

3. culture medium needed for configuration is packed into conical flask, after jumping a queue, wrapped with newspaper；

4. the above-mentioned conical flask wrapped, culture apparatus are carried out high pressure sterilization using routine techniques；

5. the culture apparatus after sterilizing and the conical flask equipped with culture medium are taken out from high-temperature sterilization pot, room temperature is placed；

6. opening inoculating hood, opens ultraviolet lamp and sterilizing 10-20 minutes is carried out to inoculating hood；Using alcohol by both hands, wrap After culture apparatus and conical flask equipped with culture medium carry out spray disinfectant, quickly by culture apparatus and equipped with the taper of culture medium Bottle is put into inoculating hood；

7. sterilized culture medium will melt in conical flask, it is cooled to 50 DEG C or so hypsokinesis and enters the culture dress after sterilizing In setting.Culture apparatus spiral is turned on, left hand holds culture dish, and the right hand holds the bottom of conical flask, left hand use simultaneously little finger of toe and Palm opens tampon, calcination bottleneck, the ware body and ware lid of culture apparatus is opened a seam with left hand thumb, until bottleneck is just It protrudes into, being poured into the amount of culture medium with culture medium thickness after solidifying is about that 4mm is advisable, and covers ware lid rapidly, is placed on table, gently revolves Turn plate, be uniformly distributed in culture medium in entire culture apparatus, after condensation plate；

8. plate culture did not occurred varied bacteria growing after 1-2 days, inoculation anaerobe can be carried out in inoculating hood, be inoculated with After ring inoculation, after ware body is connected closing into the culture plate of anaerobe by screw thread rotation with ware lid, passed through with hand interior Embedded handle pulls open rapidly sealing cover, and anaerobism agent is put into filled chamber, with i.e. by ready anaerobism bar insertion concave block Upper edge, lower edge card slot in, then let go at once, hermatic door is closed, and the oxygen in space can be realized after being adsorbed completely to be detested The Anaerobic culturel of oxygen animalcule.

Above description is the detailed description for the preferable possible embodiments of the utility model, but embodiment is not limited to The patent claim of the utility model, the same changes or modifications completed under technical spirit suggested by all the utility model Change, should belong to the covered the scope of the patents of the utility model.

Claims (7)

1. a kind of culture apparatus of Anaerobic culturel, including ware body and ware lid, it is characterised in that: the ware lid and the ware body screw thread It connects, filled chamber with an open mouth is provided in the ware lid, and filled chamber recess is set in ware lid；The filling Room is provided with the hermatic door for blocking the upper opening, and the hermatic door is concordant with the cover board of ware lid；The hermatic door and institute Filled chamber rotation connection is stated, the bottom of the filled chamber offers several through-holes, and setting one, which is placed, on the hermatic door inner wall detests The strip concave block of oxygen bar, the opening of the strip concave block surround strip concave block opening towards the bottom of filled chamber Side wall on open up there are two card slot, two card slots are oppositely arranged along the length direction of the strip concave block.

2. a kind of culture apparatus of Anaerobic culturel as described in claim 1, it is characterised in that: the hermatic door passes through rotation axis It is rotatablely connected with the filled chamber, and is socketed with torsion torsional spring in rotation axis, and torsionspring one end connects hermatic door, in addition One end connects filled chamber, so that hermatic door and filled chamber are resilient folding condition.

3. a kind of culture apparatus of Anaerobic culturel as described in claim 1, it is characterised in that: the upper limb of the upper opening is fixed There is rubber ring, the edge of the hermatic door is also fixed with corresponding rubber ring.

4. a kind of culture apparatus of Anaerobic culturel as described in claim 1, it is characterised in that: the hermatic door is also settable low In 1-3 millimeters of capping of ware lid.

5. a kind of culture apparatus of Anaerobic culturel as described in claim 1, it is characterised in that: be arranged on the hermatic door useful In the flush type handle for pulling the hermatic door.

6. a kind of culture apparatus of Anaerobic culturel as described in claim 1, it is characterised in that: the hermatic door, strip are recessed The material of block and filled chamber is that thermostable transparent plastic material is made.

7. a kind of culture apparatus of Anaerobic culturel as described in claim 1, it is characterised in that: the material of the ware lid is resistance to Temperature resistant transparent plastics or transparent glass material are made.