CN209669236U - A kind of mescenchymal stem cell separating extraction device - Google Patents
A kind of mescenchymal stem cell separating extraction device Download PDFInfo
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- CN209669236U CN209669236U CN201920072087.1U CN201920072087U CN209669236U CN 209669236 U CN209669236 U CN 209669236U CN 201920072087 U CN201920072087 U CN 201920072087U CN 209669236 U CN209669236 U CN 209669236U
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Abstract
The utility model discloses a kind of mescenchymal stem cell separating extraction devices, belong to technical field of cell separation, device includes cylinder, lid and stem cell separating bottle, lid is set at the top of cylinder, rotary electric machine and rotating cylinder are set in cylinder, radial on the outer wall of rotating cylinder to set several connecting columns for installing stem cell separating bottle, the free end of connecting column is oliquely downward extended；Stem cell separating bottle includes bottle cap, tissue bottle body and cell bottle body, and the both ends of bottle body is organized to connect respectively with bottle cap and cell bottle body, and one end that tissue bottle body is connect with cell bottle body sets strainer.The rotary electric machine of the utility model drives rotating cylinder rotation, under the influence of centrifugal force, the cell in the tiny bone tissue being placed in stem cell separating bottle is set to pass through strainer, enter in cell bottle body from tissue bottle body, and tiny bone tissue is left in tissue bottle body under the obstruction of strainer, to fast and effeciently separate cell from tiny bone tissue, structure is simple, full-featured, practical.
Description
Technical field
The utility model belongs to technical field of cell separation, and in particular to a kind of mescenchymal stem cell separating extraction device.
Background technique
At home and abroad there are the in-vitro separation and cultural method of numerous research and probe mesenchymal stem cells in recent years, it is main
There are the methods of the adherent separation of cell, density gradient centrifugation and cell sorting.The precondition of these methods is first to obtain
The marrow in ossis is taken, it is not difficult for this animal long bone such as big for big section bone, but for tiny bone, especially
It is for the bone of toy, the marrow obtained in ossis is but not easy to.In order to solve this problem, currently used method
There are two types of, one is being washed away by hand with syringe, but the operation of this method require it is fine, expend time energy, efficiency
It is not high；Such as when taking the mesenchymal stem cell of 1 week old mouse, humerus, ulna, radius, shoulder blade, femur, shin bone, calf
Bone requires to wash away, and general every bone at least needs to wash away 3-5 times repeatedly, probably needs 40-90 minutes, and since bone is thin
Small, every section of bone each section can not be thoroughly flushed to by washing away；Another kind is to use tissue adherent method, and this method is relatively easy,
Although simple, time-consuming short, its cell acquisition rate is not high, and due to adhere-wall culture, the cells such as fibroblast are easy patch
Wall climbs out of, and causes the reduction of mesenchymal stem cell purity.The measure in addition, some stem cell separation equipments do not freeze is done thin
Born of the same parents' survival rate under the higher environment of temperature is not high.
Utility model content
The purpose of the utility model is to provide a kind of mescenchymal stem cell separating extraction devices, to solve the above problems.
In order to achieve the above object, the technical solution adopted in the utility model are as follows:
A kind of mescenchymal stem cell separating extraction device, including cylinder, lid and stem cell separating bottle, the cylinder include
Lid, setting rotary electric machine and rotating cylinder in inner cylinder, the shaft edge of rotary electric machine is arranged in inner cylinder and outer barrel, the top of cylinder
The central axis direction of rotating cylinder is arranged in rotating cylinder, and several stopping-off pieces, the outer rim of stopping-off piece and the inner wall of rotating cylinder is arranged in the periphery of shaft
It is fixedly connected, radial several connecting columns of setting are for installing stem cell separating bottle, the free end of connecting column on the outer wall of rotating cylinder
Oliquely downward it is extended；The stem cell separating bottle includes bottle cap, tissue bottle body and cell bottle body, organizes the both ends point of bottle body
It is not connect with bottle cap and cell bottle body, one end that tissue bottle body is connect with cell bottle body is additionally provided with strainer.
Groove is arranged in the free end of the connecting column, and the inner sidewall of groove and the periphery of bottle cap are respectively set mutually matched
It is threadedly coupled；It is connected through a screw thread respectively between the bottle cap and tissue bottle body, between tissue bottle body and cell bottle body.
The long axis of the shaft and the central axes of rotating cylinder are overlapped, and stopping-off piece is mutually perpendicular to shaft.When guaranteeing centrifugation
Rotating cylinder keeps horizontal direction rotation, improves the centrifugation uniformity；Stopping-off piece can effectively improve the bonding strength of shaft and rotating cylinder, prevent
Only rotating cylinder glances off in centrifugal process.
Setting refrigeration sleeve on the outer wall of the inner cylinder.Refrigeration sleeve can reduce the intracorporal ring of cylinder in centrifugal operation
Border temperature improves the survival rate of cell.
Control panel is additionally provided on the outer barrel, temperature sensor, temperature sensor, refrigeration set is arranged in the bottom surface of lid
Cylinder and rotary electric machine are electrically connected with control panel respectively.Temperature sensor can monitor the intracorporal environment temperature of cylinder in real time, and
Control panel is sent data to, is made by determining whether to start by controller according to preset temperature after the analysis of microprocessor
Cold sleeve, to regulate and control the problem in cylinder；Control panel can be carried out with the experimental default revolving speed to rotary electric machine
Setting makes centrifugation reach requirement of experiment.
Microprocessor and controller are equipped with inside the control panel.
It is connected through a screw thread between the lid and cylinder, handle is arranged in the top surface of lid.Can be connected through a screw thread by
Lid is closely connect with cylinder, is prevented from throwing away at cartridge openings in centrifugal process middle cylinder body inner part and be caused danger.
Angle between the stem cell separating bottle and rotating cylinder is not more than 45 °.Guarantee that cell can stop at the end of centrifugation
Cell is caused to waste without flowing backward to strainer in the bottom for staying in cell bottle body.
Supporting leg, the bottom end setting sucker that Open Side Down of supporting leg is arranged in the bottom symmetrical of the cylinder.Sucker can be over the ground
Face is centainly adsorbed, and prevents from occurring excessively to shake in the drive lower shell of rotary electric machine and rotating cylinder in centrifugal process causing to fill
The overturning set.
The inner cylinder includes upper shell and lower shell, and rotating cylinder is arranged in upper shell, rotary electric machine is arranged in lower shell, turns
Partition of the shaft of dynamic motor between upper shell and lower shell is arranged in rotating cylinder.Lower shell to rotary electric machine and turns in separation
Cylinder is separated, and rotary electric machine can be rotated the heat generated and is relatively isolated with upper shell, the temperature in reduction between lower shell
Degree transmission influences, and the periphery that upper shell is arranged in the sleeve that freezes only only can be carried out temperature to upper shell i.e. stem cell separating bottle
Degree is adjusted, and reduces energy consumption.
The utility model having the beneficial effect that compared to the prior art
The utility model drives rotating cylinder rotation using rotary electric machine, since stem cell separating bottle is by radial installation, from
Under the action of mental and physical efforts, cell in the tiny bone tissue being placed in stem cell separating bottle is made to pass through strainer, from tissue bottle body into
Enter in cell bottle body, and tiny bone tissue is left in tissue bottle body under the obstruction of strainer, thus fast and effeciently by cell
It is separated from tiny bone tissue；
Connecting column is directly fixedly installed on the periphery of rotating cylinder by the utility model, and is uniformly arranged several, can be made
The quantity of used time Centrifuge A sample as needed, stem cell separating bottle is uniformly mounted on connecting column, due to connecting column and is done thin
Born of the same parents separate to be connected through a screw thread between flat bottle cap, and not only connection is installed and unloaded and is very convenient, will not be generated excessive
Vibration so that cell bottle intracorporal cell is caused loss cell on whipping to strainer；
The bottle cap of the utility model is organized to be connected through a screw thread respectively between bottle body and cell bottle body, convenient for it will separate before
Tiny bone tissue afterwards is put into or pours out out of tissue bottle body, and after centrifuge separation, vertical stem cell separating bottle then will be thin
Born of the same parents' bottle body is backed out from tissue bottle body bottom, does not also easily cause the unrestrained or excessive vibration of cell, and be highly convenient for by cell from
The opening of cell bottle body is poured out.
The utility model structure is simple, full-featured, practical.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the stem cell separating bottle structural schematic diagram in the utility model；
Fig. 3 is the longitudinal sectional view of the connecting column and stem cell separating bottle in the utility model；
Appended drawing reference meaning is as follows: 1, cylinder；2, inner cylinder；3, outer barrel；4, lid；5, rotary electric machine；6, rotating cylinder；7,
Shaft；8, stopping-off piece；9, connecting column；10, stem cell separating bottle；11, bottle cap；12, bottle body is organized；13, cell bottle body；14, it filters
Net；15, groove；16, freeze sleeve；17, control panel；18, temperature sensor；19, handle；20, supporting leg；21, sucker；22,
Upper shell；23, lower shell；24, partition.
Specific embodiment
The utility model is described further with reference to the accompanying drawings and detailed description.
As shown in Figure 1-3, a kind of mescenchymal stem cell separating extraction device, including cylinder, lid and stem cell separating bottle,
The cylinder 1 includes inner cylinder 2 and outer barrel 3, and lid 4 is arranged in the top of cylinder 1, and rotary electric machine 5 is arranged in inner cylinder 4 and turns
Cylinder 6, the shaft 7 of rotary electric machine 5 is arranged in rotating cylinder 6 along the central axis direction of rotating cylinder 6, and several stopping-off pieces are arranged in the periphery of shaft 7
8, the outer rim of stopping-off piece 8 is fixedly connected with the inner wall of rotating cylinder 6, and radial several connecting columns 9 of setting are for pacifying on the outer wall of rotating cylinder 6
Stem cell separating bottle 10 is filled, the free end of connecting column 9 is oliquely downward extended；The stem cell separating bottle 10 includes bottle cap
11, bottle body 12 and cell bottle body 13 are organized, the both ends of tissue bottle body 12 are connect with bottle cap 11 and cell bottle body 13 respectively, organize bottle
One end that body 12 is connect with cell bottle body 13 is additionally provided with strainer 14.
Groove 15 is arranged in the free end of the connecting column 9, and phase is respectively set in the inner sidewall of groove 15 and the periphery of bottle cap 11
The threaded connection mutually cooperated；Lead to respectively between the bottle cap 11 and tissue bottle body 12, between tissue bottle body 12 and cell bottle body 13
Cross threaded connection.
The central axes of the long axis and rotating cylinder 6 of the shaft 7 are overlapped, and stopping-off piece 8 is mutually perpendicular to shaft 7.
Setting refrigeration sleeve 16 on the outer wall of the inner cylinder 2.
Control panel 17 is additionally provided on the outer barrel 3, temperature sensor 18, temperature sensor is arranged in the bottom surface of lid 4
18, refrigeration sleeve 16 and rotary electric machine 5 are electrically connected with control panel 17 respectively.
Microprocessor and controller are equipped with inside the control panel 17.
It is connected through a screw thread between the lid 4 and cylinder 1, handle 19 is arranged in the top surface of lid 4.
Angle between the stem cell separating bottle 10 and rotating cylinder 6 is not more than 45 °.
Supporting leg 20, the bottom end setting sucker 21 that Open Side Down of supporting leg 20 is arranged in the bottom symmetrical of the cylinder 1.
The inner cylinder 2 includes upper shell 22 and lower shell 23, and rotating cylinder 6, setting in lower shell 23 are arranged in upper shell 22
Rotary electric machine 5, partition 24 of the shaft 7 of rotary electric machine 5 between upper shell 22 and lower shell 23 are arranged in rotating cylinder 6.
Before use, it is first that tiny bone shears is broken and expose myeloid tissue, need stem cell selected to use to separate according to experiment
The quantity of bottle 10 opens bottle cap 11 and tiny bone is put into tissue bottle body 12, bottle cap is caught in groove 15 after screwing on bottle cap 11
And be tightened against in connecting column 9, then lid 14 is screwed on cylinder 1, is respectively turned on control panel 17, rotary electric machine 5, refrigeration set
Cylinder 16 and temperature sensor 18(dsl8b20) power supply, temperature sensor 18 monitor cylinder 1 in environment temperature, data are passed
The microprocessor (single-chip microcontroller stm32) of control panel 17 is transported to, determines whether to lead to according to preset temperature after microprocessor analysis
Controller starting refrigeration sleeve 16 is crossed, if temperature is more than preset value in cylinder 1, controller starting refrigeration sleeve 16 is to inner cylinder
Body 2 cools down, and stops cooling when temperature drops to preset value, and temperature sensor 18 is kept to cylinder 1 in centrifugal process
Interior temperature real time monitoring carries out temperature adjustment.Experimenter can be according to requirement of experiment on control panel 17 after temperature reaches preset value
The revolving speed of rotary electric machine 5 is set and the time and is started, rotary electric machine 5 drives rotating cylinder 6 to rotate, and under the influence of centrifugal force, places
The cell in tiny bone tissue in stem cell separating bottle 10 passes through strainer 14, enters cell bottle body 13 from tissue bottle body 12
It is interior, and tiny bone tissue is left in tissue bottle body 12 under the obstruction of strainer 14, is beaten after the completion of the stalling of rotary electric machine 5 i.e. separation
It uncaps body 14, stem cell separating bottle 10 is backed out from connecting column 9 respectively, stem cell separating bottle 10 is adjusted to vertical state
Collect its content after backing out cell bottle body 13, then screw the cap off a bottle 11 will organize bottle body 12 in tiny bone do waste disposal i.e.
It can.
Claims (10)
1. a kind of mescenchymal stem cell separating extraction device, including cylinder, lid and stem cell separating bottle, it is characterised in that: institute
Stating cylinder (1) includes inner cylinder (2) and outer barrel (3), and lid (4) are arranged in the top of cylinder (1), and setting turns in inner cylinder (4)
The shaft (7) of dynamic motor (5) and rotating cylinder (6), rotary electric machine (5) is arranged in rotating cylinder (6) along the central axis direction of rotating cylinder (6), is turned
Several stopping-off pieces (8) are arranged in the periphery of axis (7), and the outer rim of stopping-off piece (8) is fixedly connected with the inner wall of rotating cylinder (6), rotating cylinder (6)
Radial several connecting columns (9) of setting are for installing stem cell separating bottle (10) on outer wall, and the free end of connecting column (9) is to obliquely downward
Side is extended；The stem cell separating bottle (10) includes bottle cap (11), tissue bottle body (12) and cell bottle body (13), organizes bottle
The both ends of body (12) are connect with bottle cap (11) and cell bottle body (13) respectively, what tissue bottle body (12) was connect with cell bottle body (13)
One end is additionally provided with strainer (14).
2. mescenchymal stem cell separating extraction device as described in claim 1, it is characterised in that: the connecting column (9) from
Groove (15) are arranged by end, mutually matched threaded connection is respectively set in the inner sidewall of groove (15) and the periphery of bottle cap (11)；
Connected respectively by screw thread between the bottle cap (11) and tissue bottle body (12), between tissue bottle body (12) and cell bottle body (13)
It connects.
3. mescenchymal stem cell separating extraction device as claimed in claim 1 or 2, it is characterised in that: the length of the shaft (7)
Axis and the central axes of rotating cylinder (6) are overlapped, and stopping-off piece (8) is mutually perpendicular to shaft (7).
4. mescenchymal stem cell separating extraction device as claimed in claim 3, it is characterised in that: outside the inner cylinder (2)
Setting refrigeration sleeve (16) on wall.
5. mescenchymal stem cell separating extraction device as claimed in claim 4, it is characterised in that: on the outer barrel (3) also
Equipped with control panel (17), temperature sensor (18) are arranged in the bottom surface of lid (4), temperature sensor (18), refrigeration sleeve (16)
It is electrically connected respectively with control panel (17) with rotary electric machine (5).
6. mescenchymal stem cell separating extraction device as claimed in claim 5, it is characterised in that: in the control panel (17)
Portion is equipped with microprocessor and controller.
7. mescenchymal stem cell separating extraction device as claimed in claim 6, it is characterised in that: the lid (4) and cylinder
(1) it is connected through a screw thread between, handle (19) are arranged in the top surface of lid (4).
8. mescenchymal stem cell separating extraction device as claimed in claim 7, it is characterised in that: the stem cell separating bottle
(10) angle between rotating cylinder (6) is not more than 45 °.
9. mescenchymal stem cell separating extraction device as claimed in claim 8, it is characterised in that: the bottom of the cylinder (1)
It is symmetrical arranged supporting leg (20), the bottom end setting sucker (21) that Open Side Down of supporting leg (20).
10. mescenchymal stem cell separating extraction device as claimed in claim 9, it is characterised in that: the inner cylinder (2) includes
Upper shell (22) and lower shell (23), upper shell (22) is interior to be arranged rotating cylinder (6), and rotary electric machine (5) are arranged in lower shell (23), turn
Partition (24) of the shaft (7) of dynamic motor (5) between upper shell (22) and lower shell (23) is arranged in rotating cylinder (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920072087.1U CN209669236U (en) | 2019-01-16 | 2019-01-16 | A kind of mescenchymal stem cell separating extraction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920072087.1U CN209669236U (en) | 2019-01-16 | 2019-01-16 | A kind of mescenchymal stem cell separating extraction device |
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| Publication Number | Publication Date |
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| CN209669236U true CN209669236U (en) | 2019-11-22 |
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| CN201920072087.1U Active CN209669236U (en) | 2019-01-16 | 2019-01-16 | A kind of mescenchymal stem cell separating extraction device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607508A (en) * | 2020-04-28 | 2020-09-01 | 深圳大学 | Automatic mesenchymal stem cell separation device |
| CN114591832A (en) * | 2022-03-28 | 2022-06-07 | 四川大学 | Animal tissue aseptic decellularization device |
-
2019
- 2019-01-16 CN CN201920072087.1U patent/CN209669236U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607508A (en) * | 2020-04-28 | 2020-09-01 | 深圳大学 | Automatic mesenchymal stem cell separation device |
| CN111607508B (en) * | 2020-04-28 | 2023-03-10 | 深圳大学 | Automatic mesenchymal stem cell separation device |
| CN114591832A (en) * | 2022-03-28 | 2022-06-07 | 四川大学 | Animal tissue aseptic decellularization device |
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