CN204281757U - A kind of homogenizer for separating of subcellular components - Google Patents
A kind of homogenizer for separating of subcellular components Download PDFInfo
- Publication number
- CN204281757U CN204281757U CN201420792054.1U CN201420792054U CN204281757U CN 204281757 U CN204281757 U CN 204281757U CN 201420792054 U CN201420792054 U CN 201420792054U CN 204281757 U CN204281757 U CN 204281757U
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- homogenate
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- homogenizer
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Abstract
The utility model discloses a kind of homogenizer for separating of subcellular components, cell or tissue block can be prepared into the homogenate comprising various subcellular components, described homogenizer comprises homogenate tube, homogenate tube comprises tube wall and is positioned at the tube core of tube wall, described tube wall top seal, pipe lid is stamped in bottom, described tube core comprises stator sleeve pipe and is arranged in the rotor of sleeve pipe, described pipe covers and is provided with rotor connection, rotor is connected with rotor connection, described homogenizer also comprises the motor being positioned at homogenate tube outside, motor by rotor connection for rotor provides rotary power.Described homogenate tube can be designed to tissue homogenate pipe or cell homogenates pipe as required, conveniently switch use, the utility model uses the automatic system with special tube core, microprocessor defines special application program by working specification optimization, make sample preparation procedure stdn, sequencing, reduce the impact of human factor, obtain subcellular structure homogenate, be separated subcellular structure for density gradient centrifugation with differential centrifugation.
Description
Technical field
The utility model relates to the separation of subcellular structure, particularly a kind of homogenizer for separating of subcellular components.
Background technology
Cell is made up of various subcellular structure, comprise nucleus, plastosome, lysosome, autophagosome, endoplasmic reticulum, golgi body etc., observe these subcellular structures or carry out biochemical function analysis, need the various subcellular components of separation and purification, centrifugation technique is the basic means of realizing this goal.Before centrifugal, need first to prepare cell homogenates or tissue homogenate.Homogenate is under cryogenic, be placed on by cell or tissue in homogenizer and add isotonic homogenate medium (i.e. 0.25mol/L sucrose-0.003mol/L calcium chloride solution) grinding, cell or tissue is mechanically ground becomes the mixture comprising various subcellular components and inclusion.
The mode of homogenate has multiple: manual homogenization, machine homogenate, ultrasonic homogenate, multigelation etc., can carry out soft homogenize process to animal vegetable tissue or cell, obtains tissue homogenate or the cell homogenates of testing needs.
Manual homogenization tissue pieces or cell is poured in glass homogenate tube together with homogenate medium or physiological saline to carry out homogenate, left hand is held homogenate tube and is filled in the vessel of mixture of ice and water by lower end insertion, stamp stem vertically inserts in sleeve pipe by the right hand, rotate tens of times of grinding (6 ~ 8 minutes) up and down, abundant grinding, makes tissue homogenate.Use glass homogenizer manual homogenization both to require great effort, time-consuming, even not again, also add the probability of pollution.Machine homogenate utilizes lapping principle, and employing machine is power, drives homogenate bar to move in homogenizer, carry out soft homogenize process to animal vegetable tissue, obtains cell homogenates or the tissue homogenate of testing needs.Although manpower has been liberated in machine homogenate, be still open-sky technique, be easy to pollute.Ultrasonication, is make cytoclasis with ultrasonic cell disruption instrument, is easy to damaging cells device, and can affects the activity of some enzyme.The cell cultivated or be separated can with above method homogenate, also can repeatedly freeze-thaw about 3 times (namely allow cell add appropriate hypotonic medium or distilled water lower in temperature refrigerator and freeze, dissolve, freeze again, dissolve again, about 3 times repeatedly), but have part enzyme activity can be influenced, and organoid release is not exclusively.
utility model content
The utility model object is: provide a kind of tissue for separating of subcellular structure or cell homogeniser, easy, quick, efficient preparation tissue or cell homogenates.
The technical solution of the utility model is:
A kind of homogenizer for separating of subcellular components, cell or tissue block is separated into subcellular structure, described homogenizer comprises homogenate tube, described homogenate tube comprises tube wall and is positioned at the tube core of tube wall, described tube wall top seal, pipe lid is stamped in bottom, described tube core comprises stator sleeve pipe and is arranged in the rotor of sleeve pipe, described pipe covers and is provided with rotor connection, rotor is connected with rotor connection, described separator also comprises the motor being positioned at homogenate tube outside, described motor by rotor connection for rotor provides rotary power.
A kind of preferred version is: be divided into Archimedean screw area under control and homogenate milling zone from top to bottom successively in the tube core of described tissue homogenizer, described Archimedean screw area under control is built with screw rod, homogenate milling zone is built with grinding rod, described screw rod and grinding rod connect to form rotor, connect rotor connection bottom grinding rod, the position of the corresponding grinding rod of stator sleeve inside pipe wall of described homogenate milling zone is provided with grinding layer.Described stator sleeve pipe base portion is provided with some equally distributed holes.Preferred further, the diameter of described hole is 2.0-3.0mm.
Another kind of preferred version is: be divided into Archimedean screw area under control, homogenate milling zone and negative pressure rotary-cut district in the tube core of described cell homogenates pipe from top to bottom successively, described Archimedean screw area under control is built with screw rod, homogenate milling zone is built with grinding rod, negative pressure rotary-cut district is built with rotary-cut cutter head, described screw rod, grinding rod and rotary-cut cutter head connect to form rotor successively, connect rotor connection bottom rotary-cut cutter head.The position of the corresponding grinding rod of stator sleeve inside pipe wall of described homogenate milling zone is provided with grinding layer.The part of the corresponding rotary-cut cutter head of stator sleeve pipe in described negative pressure rotary-cut district is provided with some equally distributed holes.Preferred further, the diameter of described hole is 0.8-1.2mm.
For above-mentioned two schemes:
Preferably, described rotor is sealed by the joint of rotor connection and pipe lid.
Preferably, described homogenate tube outside is also provided with protection stay-warm case.
Preferred further, described protection stay-warm case is built with temp probe and refrigeration compressor, and described temp probe and refrigeration compressor are connected with a microprocessor respectively, and described refrigeration compressor is also connected with air-circulating fan; Described microprocessor is also connected to motor and a touch screen.
The utility model has the advantages that:
1. the homogenizer for separating of subcellular structure provided by the utility model, with the use of tissue homogenate pipe or cell homogenates pipe, built-in special tube core, composition automatic system, microprocessor defines special application program (time, speed, direction) by working specification optimization, can process sample easily, make sample preparation procedure stdn, sequencing, reduce the impact of human factor, becoming operator's dependency is no-operation person dependency.
2. homogenizer of the present utility model can select different homogenate tubes as required, when sample is tissue block, can adopt with the trizonal tissue homogenate pipe in Archimedean screw area under control, homogenate milling zone and negative pressure rotary-cut district, directly can select the cell homogenates pipe with Archimedean screw area under control, Liang Ge region, homogenate milling zone when sample is single cell suspension, switch and use flexibly.During using-system homogenate tube, required tissue samples only simply need be prepared into the tissue block of several mm in size before separation, and the peeling knife in tissue homogenate pipe can be peeled into 1.0mm automatically
3the tissue fragments of size, and then be prepared into subcellular structure homogenate by homogenate milling zone, can be used for density gradient centrifugation and be separated subcellular structure with differential centrifugation.
3. the utility model is by the specific program of setting, and control speed, time and direction, the utility model also can be used for preparing tissue protein homogenate.
4. the utility model has protection stay-warm case, and control realization protection insulation case temperature is at 0-4 DEG C, and Cell protection device and protein function, provide sealed aseptic Working environment simultaneously, ensures user's safety in utilization.
5. the utility model operation is gentle, and easy, quick, efficient.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is further described:
Fig. 1 is the structural principle of the homogenizer for separating of subcellular structure described in the utility model;
Fig. 2 is the structural representation of tissue homogenate pipe described in the utility model;
Fig. 3 is the exploded view of the pipe of tissue homogenate shown in Fig. 2;
Fig. 4 is the structural representation of cell homogenates pipe described in the utility model;
Fig. 5 is the exploded view of the pipe of cell homogenates shown in Fig. 4;
Fig. 6 is the schematic diagram of temp probe described in the utility model.
Embodiment
As shown in Figure 1, the homogenizer for tissue block or cell homogenates being separated into subcellular structure that the utility model discloses, comprises homogenate tube, below homogenate tube, motor is housed, motor speed is adjustable, can reverse, and can operate according to set program (time, speed, direction).Described homogenate tube outside is also provided with protection stay-warm case; described protection stay-warm case is built with temp probe, refrigeration compressor and air-circulating fan; described temp probe, refrigeration compressor and air-circulating fan are connected with a microprocessor respectively; realize temperature automatically controlled 0-4 DEG C in protection stay-warm case, Cell protection device and protein function.Microprocessor also connects motor, controls speed of rotation and the direction of motor, described microprocessor also connects a touch screen, is used for inputting situation in operating parameter and display operational process.
embodiment 1
As shown in Figures 2 and 3, provide a kind of tissue homogenate pipe, when sample is tissue block, this tissue homogenate pipe can be adopted, described tissue homogenate pipe, comprise tube wall 1 and be positioned at the tube core 2 of tube wall, described tube wall top seal, pipe lid 3 is stamped in bottom, described tube core 2 comprises stator sleeve pipe 4 and is arranged in the rotor 5 of sleeve pipe 4, described pipe lid 3 is provided with rotor connection, rotor is connected with rotor connection, described rotor is sealed by the joint of rotor connection and pipe lid, motor below homogenate tube by rotor connection for rotor provides rotary power, stator sleeve pipe 4 and tube wall are fixed on motor by bayonet socket.
Described tube core 2 is divided into Archimedean screw area under control, homogenate milling zone and negative pressure rotary-cut district from top to bottom successively, and the corresponding negative pressure rotary-cut district of stator casing part of tube core is provided with the hole 6 that some equally distributed diameters are 0.8-1.2mm.Described Archimedean screw area under control is built with screw rod 51, homogenate milling zone is built with grinding rod 52, negative pressure rotary-cut district is built with rotary-cut cutter head 53, and described screw rod 51, grinding rod 52 and rotary-cut cutter head 53 connect to form rotor 5 successively, connect rotor connection bottom rotary-cut cutter head 53.
Wherein: Archimedean screw area under control: the screw rod structure of rotor forms Archimedean screw pipe jointly with stator sleeve pipe, utilize sleeve pipe cylinder internal screw to take turns turn rising and promote liquid, its principle of work is when internal screw rotates, screw rod rotates around the axis of itself on the one hand, it rolls along sleeve inner surface again on the other hand, so form the sealed chamber of liquid.Screw rod often circles, and the water in annular seal space pushes ahead a pitch, and with the continuous rotation of work screw rod, water presses to another annular seal space from an annular seal space, is finally extruded from cannula tip by liquid, meanwhile, forms negative pressure, cause suction at sleeve bottom.
Negative pressure rotary-cut district: the suction that negative pressure is formed makes tissue block be close to casing wall, and sinks into hole, and the tissue cutting be absorbed in from cavity gets off by the rotary-cut cutter head of high speed rotating, forms 1.0mm
3the tissue fragments of size.
Homogenate milling zone: tissue fragments, under the extracting effect of negative pressure, rises with liquid stream, through homogenate milling zone, the teflon grinding layer grinding of the teflon homogenate working face on polished rod and tube core inwall is broken for subcellular structure homogenate.
Liquid in homogenate tube carries tissue fragments and constantly enters from the aperture of stator sleeve bottom, respectively through negative pressure rotary-cut district, homogenate milling zone and Archimedean screw area under control, flow out from cannula tip, so go round and begin again, tissue grinder is broken for subcellular structure homogenate the most at last.Be designed with Umklapp process in tissue homogenate program, when rotor reverses, the flow direction in tube core reverses, and tissue pieces can be avoided to block the hole in negative pressure rotary-cut district.
The sample quality scope that the utility model is separated: 20-4000mg; Rotor speed: 50-1000rpm, can rotate by both forward and reverse directions; Time: 1-600 second; Temperature: temperature controllable is in 0-4 DEG C; Application program: require working specification and homogenate program that optimization is provided according to different tissue block features and homogenate; Sample disposal: be aseptic, airtight space in tissue homogenate pipe.
Temp probe of the present utility model, refrigeration compressor and air-circulating fan realize object of freezing jointly.
As shown in Figure 6, the temperature measuring head of temp probe of the present utility model adopts digital temperature sensor.This sensor is by function i ntegration such as temperature sensing, signal conversion, A/D conversions on a chip, and its internal structure as shown in Figure 6.Temperature transition is become electrical signal by this chip, and first this electrical signal enters small signal amplifier and amplify; Then the A/D converter of 14 is entered; Eventually pass two-wire serial digital interface output digit signals.This sensor has a calibration register can in measuring process, and calibration factor can carry out the signal of sensor by automatic calibration.
embodiment 2
As shown in Figures 4 and 5, provide a kind of cell homogenates pipe, when sample is single cell suspension, this cell homogenates pipe can be adopted.Described cell homogenates pipe comprises tube wall 1 ' and is positioned at the tube core 2 ' of tube wall, described tube wall top seal, pipe lid 3 ' is stamped in bottom, described tube core 2 ' comprises stator sleeve pipe 4 ' and is arranged in the rotor 5 ' of sleeve pipe 4 ', described pipe lid 3 ' is provided with rotor connection, and rotor is connected with rotor connection, and described rotor is sealed by the joint of rotor connection and pipe lid, motor below separator tube is by rotor connection for rotor provides rotary power, and stator sleeve pipe and tube wall are fixed on motor by bayonet socket.
In described tube core, 2 ' is divided into Archimedean screw area under control and homogenate milling zone from top to bottom successively, and it is the hole 6 ' of 2-3mm that the stator sleeve pipe base portion of tube core is provided with some equally distributed diameters.Described Archimedean screw area under control is built with screw rod 51 ', and homogenate milling zone is built with grinding rod 52 ', and described screw rod 51 ' and grinding rod 52 ' connect to form rotor 5 ', and bottom connects rotor connection.Archimedean screw area under control is identical with embodiment 1 with the function of homogenate milling zone.
The size of a sample scope of cell homogenates pipe: 20-40ml; Rotor speed: 50-1000rpm, can rotate by both forward and reverse directions; Time: 1-600 second; Temperature: temperature controllable is in 0-4 DEG C; Application program: working specification and homogenate program that optimization is provided according to different subcellular structure separation requirement; Sample disposal: be aseptic, airtight space in homogenate tube.
The temprature control unit of the present embodiment is identical with embodiment 1.
Above-described embodiment, only for technical conceive of the present utility model and feature are described, its object is to person skilled in the art can be understood content of the present utility model and implement according to this, can not limit protection domain of the present utility model with this.All modifications done according to the spirit of the utility model main technical schemes, all should be encompassed within protection domain of the present utility model.
Claims (10)
1. the homogenizer for separating of subcellular components, cell or tissue block is prepared into the cell homogenates or tissue homogenate that comprise various subcellular components, described homogenizer comprises homogenate tube, homogenate tube comprises tissue homogenate pipe and cell homogenates pipe, its common trait is: described homogenate tube comprises tube wall and is positioned at the tube core of tube wall, described tube wall top seal, pipe lid is stamped in bottom, described tube core comprises stator sleeve pipe and is arranged in the rotor of sleeve pipe, described pipe covers and is provided with rotor connection, rotor is connected with rotor connection, described homogenizer also comprises the motor being positioned at homogenate tube outside, described motor by rotor connection for rotor provides rotary power.
2. the cell homogeniser for separating of subcellular components according to claim 1, it is characterized in that: in described tube core, be divided into Archimedean screw area under control and homogenate milling zone from top to bottom successively, described Archimedean screw area under control is built with screw rod, homogenate milling zone is built with grinding rod, described screw rod and grinding rod connect to form rotor, connect rotor connection bottom grinding rod, the region of the corresponding grinding rod of stator sleeve inside pipe wall of described homogenate milling zone is provided with grinding layer.
3. the tissue homogenizer for separating of subcellular components according to claim 1, it is characterized in that: in described tube core, be divided into Archimedean screw area under control and homogenate milling zone and negative pressure rotary-cut district from top to bottom successively, described Archimedean screw area under control is built with screw rod, homogenate milling zone is built with grinding rod, negative pressure rotary-cut district is built with rotary-cut cutter head, described screw rod, grinding rod and rotary-cut cutter head connect to form rotor successively, connect rotor connection bottom rotary-cut cutter head, the position of the corresponding grinding rod of stator sleeve inside pipe wall of described homogenate milling zone is provided with grinding layer.
4. the cell homogeniser for separating of subcellular components according to claim 2, is characterized in that: described stator sleeve pipe base portion is provided with some equally distributed holes.
5. the homogenizer for separating of subcellular components according to claim 4, is characterized in that: the diameter of described hole is 2.0-3.0mm.
6. the tissue homogenizer for separating of subcellular components according to claim 3, is characterized in that: the part in the corresponding negative pressure rotary-cut district of described stator sleeve pipe is provided with some equally distributed holes.
7. the tissue homogenizer for separating of subcellular components according to claim 6, is characterized in that: the diameter of described hole is 0.8-1.2mm.
8. the homogenizer for separating of subcellular components according to claim 1, is characterized in that: described rotor is sealed by the joint of rotor connection and pipe lid.
9. the homogenizer for separating of subcellular components according to Claims 2 or 3, is characterized in that: described homogenate tube outside is also provided with protection stay-warm case.
10. the homogenizer for separating of subcellular components according to Claims 2 or 3, it is characterized in that: described protection stay-warm case is built with temp probe and refrigeration compressor, described temp probe and refrigeration compressor are connected with a microprocessor respectively, and described refrigeration compressor is also connected with air-circulating fan; Described microprocessor is also connected to motor and a touch screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420792054.1U CN204281757U (en) | 2014-12-16 | 2014-12-16 | A kind of homogenizer for separating of subcellular components |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420792054.1U CN204281757U (en) | 2014-12-16 | 2014-12-16 | A kind of homogenizer for separating of subcellular components |
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| CN204281757U true CN204281757U (en) | 2015-04-22 |
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| CN201420792054.1U Expired - Fee Related CN204281757U (en) | 2014-12-16 | 2014-12-16 | A kind of homogenizer for separating of subcellular components |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498350A (en) * | 2014-12-16 | 2015-04-08 | 何向锋 | Homogenizer for separating subcellular fractions |
| CN107497570A (en) * | 2017-09-21 | 2017-12-22 | 贵州大学 | Homogenate tube is organized to fix lapping device |
| US11033295B2 (en) | 2019-05-06 | 2021-06-15 | Tissuemill Technologies Llc | Atraumatically formed tissue composition, devices and methods of preparation and treatment |
-
2014
- 2014-12-16 CN CN201420792054.1U patent/CN204281757U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498350A (en) * | 2014-12-16 | 2015-04-08 | 何向锋 | Homogenizer for separating subcellular fractions |
| CN107497570A (en) * | 2017-09-21 | 2017-12-22 | 贵州大学 | Homogenate tube is organized to fix lapping device |
| US11033295B2 (en) | 2019-05-06 | 2021-06-15 | Tissuemill Technologies Llc | Atraumatically formed tissue composition, devices and methods of preparation and treatment |
| US11918248B2 (en) | 2019-05-06 | 2024-03-05 | Tissuemill Technologies Llc | Atraumatically formed tissue compositions, devices and methods of preparation and treatment |
| US11963695B2 (en) | 2019-05-06 | 2024-04-23 | Tissuemill Technologies Llc | Atraumatically formed tissue compositions, devices and methods of preparation and treatment |
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150422 Termination date: 20161216 |