CN209584269U

CN209584269U - A kind of water-bath suitable for Cytology Lab cell culture

Info

Publication number: CN209584269U
Application number: CN201920207950.XU
Authority: CN
Inventors: 易小厅; 尹利萍; 黄珂
Original assignee: Chengdu Hui Hui Biotechnology Co Ltd
Current assignee: Chengdu Hui Hui Biotechnology Co Ltd
Priority date: 2019-02-18
Filing date: 2019-02-18
Publication date: 2019-11-05
Anticipated expiration: 2029-02-18

Abstract

The utility model belongs to Bioexperiment equipment technical field, discloses a kind of water-bath suitable for Cytology Lab cell culture, including water-bath ontology and setting in the intrinsic revolving part of the water-bath；The revolving part includes support rod, cell fixed disk and the cell cryopreservation pipe cap being independently arranged；One end of the support rod is connect with the bottom wall of the sink of the water-bath ontology；The upper end of the cell fixed disk and the support rod rotates and is detachably connected, and the cell fixed disk is provided with multiple cell pipe through-holes.Utility model has the advantages that by the way that rotatable cell fixed disk is arranged, and it is independently arranged cell cryopreservation pipe cap, when carrying out cell recovery, cell pipe through-hole is placed in after cell cryopreservation tube directly being put on cell cryopreservation pipe cap, cell fixed disk is rotated, it can fast melt recovery cell.

Description

A kind of water-bath suitable for Cytology Lab cell culture

Technical field

The utility model belongs to Bioexperiment equipment technical field, and in particular to a kind of suitable for Cytology Lab cell culture Water-bath.

Background technique

Water-bath is mainly used for distillation in laboratory, dry, concentration and heating water bath chemicals or biological products, wherein Using most wide for thermostat water bath, existing thermostat water bath is general are as follows: the stainless steel tubular heater of built-in level, sink Inside be placed with aluminum shelf with holes, be furnished with the combination lasso of different bores on upper lid, be suitable for the flask of different bores.Water There is discharge tube on the left of bath, has electric box on the right side of thermostat water bath, temperature control instrument and power supply are housed on electric box front panel Switch etc., electric box is interior, and there are also electrothermal tube and sensors.Thermostat water bath has that temperature control is sensitive, reliable performance, easy to use Feature is biology, heredity, virus, aquatic products, environmental protection, medicine, health, laboratory, the indispensable work in analysis room and education and scientific research Tool.

In the analyses experiments such as biology, heredity, virus or scientific research activity, cell culture is often referred to, cell culture needs Excellent cell recovery is wanted to operate, to guarantee the in good condition of initial cell, while the sterile working that subsequent needs are stringent, simultaneously It also include being had the disadvantage that the stringent sterilization for using article using existing thermostat water bath auxiliary cell culture

(1) one of most important link is cell recovery in cell culture.Cell recovery needs (- 196 from liquid nitrogen DEG C) take out the cell that freezes and be directly immersed in 37 DEG C of warm water, and shake frequently and it is enabled to melt as early as possible, it is used immediately after cell thawing Suction pipe is sucked out cell suspension and carries out subsequent operation, and the core element of the process is " speed is melted ", i.e. heating is fast, prevents from thawing Moisture enters cell in journey, forms ice crystal, influences cell survival.Currently, laboratory technician's recovery cell generally uses tweezers to clamp jelly Pipe pipe cap is deposited, is shaken in water-bath to melt cell rapidly.Since cell cryopreservation tube is to be removed from liquid nitrogen to be immediately placed in water In bath, pipe cap may occur due to the temperature difference and not screw, clamp cryopreservation tube using tweezers and shake, be easy to make to freeze water inlet tube, It is abandoned so that valuable cell is contaminated.In addition, being shaken if screwing cryopreservation tube before being put into water-bath being clamped with tweezers When dynamic, spray is also easy to splash cryopreservation tube nozzle, since the indoor thermostat water bath of cell shares for many people, it is understood that there may be one Fixed pollution is easy cell contamination occur in subsequent cell incubation.It is answered in addition, clamping cryopreservation tube using tweezers Soviet Union is once only capable of one cryopreservation tube of folder, and conventional efficient is lower, or only allows most two people while water-bath being used to carry out cell Recovery arranges experiment more Cytology Lab, hence it is evident that be an impediment to the experiment arrangement of most people.

(2) other than cell recovery, the sterile working in cell cultivation process is also the successful key factor of cell culture. It in cell cultivation process, generally requires and cell culture medium or reagent treatment is preheated, to reduce the stimulation to cell.So And current thermostat water bath generally only has a groove, when laboratory technician needs to preheat, culture bottle is put into and is wherein carried out in advance Heat.Since in the same Cytology Lab, the experiment type or progress of each laboratory technician is different, and the reagent or culture medium for needing to preheat are not Identical to the greatest extent, that is, the water possessed in the groove needed is different.For convenience of everybody operation, can generally keep in groove in water level.This So that there are the following problems when pre- thermal agent such as cell culture medium: when preheating one bottle of new culture medium, bottle can sink to water Trench bottom, the water in groove may be contacted directly with bottleneck, be polluted；When the culture medium that preheating has used fastly, bottle meeting It swims on the water surface, will also result in certain pollution.

Therefore, if a kind of thermostat water bath suitable for cell culture chamber can be developed, the behaviour of laboratory technician will be greatly reduced It makes mistakes.

Utility model content

In order to solve the problems, such as above-mentioned " being easy water inlet when cell recovery " of the existing technology, the utility model aim is A kind of water-bath suitable for Cytology Lab cell culture is provided.

The technology employed by the present utility model is

A kind of water-bath suitable for Cytology Lab cell culture, including water-bath ontology and setting are in the water-bath ontology Interior revolving part；The revolving part includes support rod, cell fixed disk and the cell cryopreservation pipe cap being independently arranged；The support rod One end connect with the bottom wall of the sink of the water-bath ontology；The upper end rotation of the cell fixed disk and the support rod and It is detachably connected, the cell fixed disk is provided with multiple cell pipe through-holes, and the outer diameter of the cell cryopreservation pipe cap is greater than described The diameter of cell pipe through-hole.

Preferably, above-mentioned water-bath is additionally provided with multiple centrifugation pipe through-holes in the cell fixed disk.

Preferably, above-mentioned water-bath, the revolving part further include that the culture solution being slidably arranged on the support rod is fixed Disk is provided with the spring mountings of fixed culture liquid in the culture solution fixed disk.

Preferably, above-mentioned water-bath, the culture solution fixed disk are provided centrally with through-hole, set on the side wall of the through-hole It is equipped with slide protrusion, is arranged fluted on the side wall of the support rod, spring, one end of the spring are provided in the groove It is fixedly connected with the lower wall of the groove, the other end of the spring is fixedly connected with the lower bottom part of the slide protrusion.

Preferably, the quantity of above-mentioned water-bath, the slide protrusion, groove and spring is equal and is two or more.

Preferably, above-mentioned water-bath, the quantity of the revolving part are two or more.

Preferably, above-mentioned water-bath is provided with more than one hook on the side wall of the water-bath ontology.

The utility model has the following beneficial effects:

Water-bath provided by the utility model passes through the cell cryopreservation that rotatable cell fixed disk is arranged and is independently arranged Pipe cap is placed in cell pipe through-hole after cell cryopreservation tube directly being put on cell cryopreservation pipe cap when carrying out cell recovery, rotates Cell fixed disk, can fast melt recovery cell.Water-bath provided by the utility model can not only solve appearance when cell recovery The problem of easily intaking, moreover it is possible to which difficulty is fixed when reagent heating water bath needed for solving the problems, such as culture medium or other cell culture.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of the water-bath of the utility model；

Fig. 2 is the structural schematic diagram of the revolving part of the water-bath of the utility model.

In figure: 1- water-bath ontology；11- hook；2- revolving part；21- support rod；211- groove；22- cell fixed disk； 221- cell pipe through-hole；222- is centrifuged pipe through-hole；23- cell cryopreservation pipe cap；24- culture solution fixed disk；241- slide protrusion； 242- spring mountings；242- spring mountings；25- spring.

Specific embodiment

With reference to the accompanying drawing and specific embodiment is further elaborated the utility model.

Embodiment 1

As shown in Figure 1, water-bath provided in this embodiment, specific as follows:

A kind of water-bath suitable for Cytology Lab cell culture, including water-bath ontology 1 and setting are in the water-bath sheet Revolving part 2 in body 1；The revolving part 2 includes support rod 21, cell fixed disk 22 and the cell cryopreservation pipe cap being independently arranged 23；The upper end of the cell fixed disk 22 and the support rod 21 rotates and is detachably connected, and sets in the cell fixed disk 22 It is equipped with multiple cell pipe through-holes 221.

Rotating and being detachably connected between support rod 21 and cell fixed disk 22 to be arranged as follows:

Mode one: groove is arranged in the top of support rod 21, and cylindrical bump is arranged in the lower wall of cell fixed disk 22, cylindric Protrusion and groove are adapted, and the diameter of cylindrical bump is slightly less than groove, allow the cell fixed disk 22 to be with support rod 21 Axis arbitrarily rotates.

Mode two: groove is arranged in the lower wall of cell fixed disk 22, and support rod 21 is cylinder, and top is embedded in groove, branch Strut 21 and groove are adapted, and the diameter of support rod 21 is slightly less than groove, allow the cell fixed disk 22 to be with support rod 21 Axis arbitrarily rotates.

When do not use cell fixed disk 22 when, can be removed, so as to preheat other reagents such as culture medium, physiological saline and Serum etc..

One end of support rod 21 is connect with the bottom wall of the sink of the water-bath ontology 1, and connection type between the two is excellent Be selected as: the inner surface of the bottom wall of sink is arranged to lower groove or upward protrusion, and groove is arranged in raised upper surface, these two types of recessed The surface of slot is respectively provided with internal screw thread or external screw thread, and the surface of the lower end of support rod 21 is also provided with external screw thread or internal screw thread, the two spiral shell Line connection.

In addition, the outer diameter of the cell cryopreservation pipe cap 23 is greater than the diameter of the cell pipe through-hole 221, cell cryopreservation pipe cap 23 are preferably made of an elastomeric material, and internal diameter should be less than the outer diameter of cell cryopreservation tube, tightly to cover on cell cryopreservation tube. The cell cryopreservation tube just taken out is put on into cell cryopreservation pipe cap 23, is put into cell pipe through-hole 221, the bottom of cryopreservation tube enters 37 DEG C Water in, top is cased with cell cryopreservation pipe cap 23, will not slide entirely over cell pipe through-hole 221 enter water in, i.e., will not intake, protect When demonstrate,proving cell recovery, the cleaning of cell cryopreservation tube nozzle reduces the possibility of pollution.

Embodiment 2

A kind of water-bath suitable for Cytology Lab cell culture, including water-bath ontology 1 and setting are in the water-bath sheet Revolving part 2 in body 1；The revolving part 2 includes support rod 21, cell fixed disk 22 and the cell cryopreservation pipe cap being independently arranged 23；One end of the support rod 21 is connect with the bottom wall of the sink of the water-bath ontology 1；The cell fixed disk 22 with it is described The upper end of support rod 21 rotates and is detachably connected, and multiple cell pipe through-holes 221 are provided in the cell fixed disk 22.

Due to not having cell recovery singly in Cytology Lab, the preheating of other reagents is also related in cell cultivation process, this A little reagents can use the pipe of the different sizes such as 1.5mL centrifuge tube, 2mL centrifuge tube, 15mL centrifuge tube and 50mL centrifuge tube, therefore, The centrifugation pipe through-hole 222 of multiple and different specifications is additionally provided in the cell fixed disk 22, to be suitable for these centrifuge tubes Preheating.

Embodiment 3

As shown in Figs. 1-2, water-bath provided in this embodiment, specific as follows:

Preferably, above-mentioned water-bath, the revolving part 2 further include that the culture solution being slidably arranged on the support rod 21 is consolidated Price fixing 24.The culture solution fixed disk 24 is provided centrally with through-hole, and slide protrusion 241 is provided on the side wall of the through-hole, Fluted 211 are arranged on the side wall of the support rod 21, spring 25, one end of the spring 25 are provided in the groove 211 It is fixedly connected with the lower wall of the groove, the lower bottom part of the other end of the spring 25 and the slide protrusion 241 is fixed to be connected It connects.

In addition, being provided with spring mountings 242 in culture solution fixed disk 24, the purpose of spring mountings 242 is to block The reagent bottle of large volume, prevents it because buoyancy moves up, bottleneck is caused to pollute.

Spring mountings 242 are preferably such as under type:

Spring mountings 242 can be ring-spring, be connected with connecting spring on ring-spring, the other end of connecting spring with Culture solution fixed disk 24 is fixedly connected, and the other end is fixedly connected with ring-spring, ring-spring for block culture bottle or other Reagent bottle.

Spring mountings 242 can be separately positioned on square or rectangular for four fastening pawls of sliding, four fastening pawls Four points on, can be in culture solution fixed disk 24 along the diagonal slide of square or rectangular.Fastening pawl pawl be preferably Arc.

Embodiment 4

Preferably, above-mentioned water-bath, the revolving part 2 further include that the culture solution being slidably arranged on the support rod 21 is consolidated Price fixing 24.The culture solution fixed disk 24 is provided centrally with through-hole, and slide protrusion 241 is provided on the side wall of the through-hole, It is arranged fluted on the side wall of the support rod 21, is provided with spring in the groove, one end of the spring and the groove Lower wall be fixedly connected, the other end of the spring is fixedly connected with the lower bottom part of the slide protrusion.The slide protrusion 241, the two or more equal and equal with the quantity of spring 25 of groove 211.I.e. multiple slide protrusions 241 are with culture solution fixed disk 24 Center be axisymmetrical be arranged on the side wall of through-hole, the groove 211 on support rod 21 also with support rod 21 in the vertical direction Axisymmetrical be arranged on the side wall of support rod 21, the position of groove 211 and slide protrusion is adapted.

For the ease of carrying out the preheating of plurality of reagents or cell simultaneously, the quantity of the revolving part 2 is two or more.

More than one hook 11 is provided on the side wall of the water-bath ontology 1, not need cell fixed disk 22 When, it can be removed and be suspended on hook 11, linked up with 11 quantity and be adapted with the quantity of cell fixed disk 22.

Embodiment 5

The centrifugation pipe through-hole 222 of multiple and different specifications is additionally provided in the cell fixed disk 22.The revolving part 2 also wraps Include the culture solution fixed disk 24 being slidably arranged on the support rod 21.Being provided centrally with for the culture solution fixed disk 24 is logical Hole is provided with slide protrusion on the side wall of the through-hole, is arranged fluted on the side wall of the support rod 21, sets in the groove It is equipped with spring, one end of the spring is fixedly connected with the lower wall of the groove, the other end of the spring and the sliding The lower bottom part of protrusion is fixedly connected.The quantity of the slide protrusion, groove and spring is equal and equal two or more.The water-bath More than one hook 11 is provided on the side wall of ontology 1.The quantity of the revolving part 2 is two or more.The support training of spring 25 Nutrient solution fixed disk 24 is located at the top of groove 211, when putting liquid in culture solution fixed disk 24, culture solution fixed disk 24 can glide a certain distance under the support of spring 25, but not be snapped down to the bottom of groove 211 completely, can guarantee culture solution The bottleneck of bottle avoids bottleneck from gluing water contaminated in water-bath ullage.

The application method for the water-bath that embodiment 5 provides is as follows:

Recovery cell: putting on cell cryopreservation pipe cap 23 for the cell cryopreservation tube just taken out, and is put into cell pipe through-hole 221, rotation Turn cell fixed disk 22, melts cell.

Pre- thermal agent: the centrifuge tube for filling reagent or other pipes are put into the centrifugation pipe through-hole that suitable specification matches 222, Spin cells fixed disk 22, pre- thermal agent.

It heats culture medium biggish reagent in equal volume: removing cell fixed disk 22, the reagent bottle of large volume is placed on training In nutrient solution fixed disk 24, the spring mountings 242 in culture solution fixed disk 24 are pulled open, make the reagent bottle subcard of large volume at it In, prevent it because buoyancy moves up, it can pre- thermal agent.

The utility model is not limited to above-mentioned optional embodiment, anyone can obtain under the enlightenment of the utility model Other various forms of products, however, making any variation in its shape or structure, all the utility model rights that falls into are wanted The technical solution in confining spectrum is sought, is all fallen within the protection scope of the utility model.

Claims

1. a kind of water-bath suitable for Cytology Lab cell culture, it is characterised in that: including water-bath ontology (1) and be arranged in institute State the revolving part (2) in water-bath ontology (1)；The revolving part (2) includes support rod (21), cell fixed disk (22) and independence The cell cryopreservation pipe cap (23) of setting；The bottom wall of the sink of one end of the support rod (21) and the water-bath ontology (1) connects It connects；The upper end of the cell fixed disk (22) and the support rod (21) rotates and is detachably connected, the cell fixed disk (22) multiple cell pipe through-holes (221) are provided on, the outer diameter of the cell cryopreservation pipe cap (23) is greater than the cell pipe through-hole (221) diameter.

2. water-bath according to claim 1, it is characterised in that: be additionally provided on the cell fixed disk (22) it is multiple from Heart pipe through-hole (222).

3. water-bath according to claim 1 or 2, it is characterised in that: the revolving part (2) further includes being slidably arranged in institute The culture solution fixed disk (24) on support rod (21) is stated, the bullet of fixed culture liquid is provided on the culture solution fixed disk (24) Spring fixing piece (242).

4. water-bath according to claim 3, it is characterised in that: being provided centrally with for the culture solution fixed disk (24) is logical Hole is provided with slide protrusion (241) on the side wall of the through-hole, fluted (211) is arranged on the side wall of the support rod (21), It is provided with spring (25) in the groove (211), one end of the spring (25) and the fixed company of the lower wall of the groove (211) It connects, the other end of the spring (25) is fixedly connected with the lower bottom part of the slide protrusion (241).

5. water-bath according to claim 4, it is characterised in that: the slide protrusion (241), groove (211) and spring (25) quantity is equal and is two or more.

6. water-bath according to claim 1, it is characterised in that: the quantity of the revolving part (2) is two or more.

7. water-bath according to claim 1, it is characterised in that: be provided with one on the side wall of the water-bath ontology (1) A above hook (11).