CN110220758A - Sputum sample leaves and takes the method that mycobacterium tuberculosis is separated in cup and sputum - Google Patents
Sputum sample leaves and takes the method that mycobacterium tuberculosis is separated in cup and sputum Download PDFInfo
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- CN110220758A CN110220758A CN201910518508.3A CN201910518508A CN110220758A CN 110220758 A CN110220758 A CN 110220758A CN 201910518508 A CN201910518508 A CN 201910518508A CN 110220758 A CN110220758 A CN 110220758A
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- sputum sample
- cup
- sputum
- lid
- protrusion
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0096—Casings for storing test samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
- G01N2001/1427—Positive displacement, piston, peristaltic
Abstract
The invention discloses a kind of sputum samples to leave and take the method that mycobacterium tuberculosis is separated in cup and sputum, it includes the cup body with accommodating cavity that sputum sample, which leaves and takes cup, offer the first lid of liquid outlet, second lid of lid envelope liquid outlet, connect the link block and block of cup body, and the drainage elastic slice having a first end and a second end, first end connects link block, second end is supported with block from below, mutual disconnected first chamber and second chamber are divided into so that cavity will be accommodated, second end can be under the gravity of sputum sample far from block, and by sputum sample pilot flow direction second chamber, second end can be sprung back after sputum sample flows into second chamber to be resetted.In this way, the sputum sample being stored in second chamber will not cause nausea etc. to experience to personnel, sample will not pollute the first lid or the second lid when shaking up, and reduce the excessive infected person of sample or pollute the risk of table top.The method that mycobacterium tuberculosis is separated in the sputum, leaves and takes cup using above-mentioned sputum sample.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of sputum sample to leave and take in cup and sputum and separate tuberculosis
The method of mycobacteria.
Background technique
In medicine or biological research fields, sputum sample leaves and takes cup and refers to the sputum to be detected taken for storing acquisition
The container of sample.Existing sputum sample leaves and takes cup, no matter which kind of specification and size, because of the limitation of itself structure, right
When the sputum sample for class of volatilizing carries out accessing operation, often there is higher infection risk.
For example, in the process for separating mycobacterium tuberculosis from sputum sample, it is necessary first to fetch sputum from patient
Deposit in and leave and take in cup, cup then will be left and taken by experimenter and open and inject liquefied reagent, by shaking up, then sample again into
Row centrifuge separation, wherein in injection liquefied reagent and sampling process, the rim of a cup for leaving and taking cup is all opened wide, and there are biggish
Infection risk, and experimenter can be easily seen the sputum left and taken in cup, cause the bad experience such as nausea；During shaking up,
Sputum can also adhere to and on cup lid, improve sputum volatilization infection experiment personnel and pollute the risk of experiment table top.
Summary of the invention
The purpose of the present invention is to provide a kind of sputum samples to leave and take cup, leaves and takes phlegm in cup to solve current sputum sample
Liquid sample is easy the Infection Action personnel in access procedure, and the technical problem on cup lid can be attached to during shaking up.
To achieve the above object, the technical solution adopted by the present invention is that: a kind of sputum sample is provided and leaves and takes cup, comprising:
Cup body has accommodating cavity；
First lid seals the accommodating cavity for covering, offers liquid outlet on first lid；
Second lid, removably lid seals the liquid outlet；
Link block is connected to the cup body, and is located in the accommodating cavity；
Block is connected to the cup body, and is located in the accommodating cavity；And
Elastic slice is drained, the drainage elastic slice has the first end and second end being oppositely arranged, and the first end is connected to institute
Link block is stated, the second end is supported with the block from below, and the accommodating cavity is divided into mutual disconnected first
Chamber and second chamber, the second end can be under the gravity of the sputum sample far from the block, and by the phlegm
Second chamber described in liquid sample pilot flow direction, the drainage elastic slice can be automatic after the sputum sample flows into the second chamber
Rebound resets.
Further, first lid is detachably connected with the cup body, seals the accommodating cavity with lid.
Further, the link block and the block form difference in height on gravity direction, and the link block is compared with institute
State the top that block is closer to the cup body.
Further, on gravity direction, the link block and the block are mutually staggered with the liquid outlet.
Further, the oval sheet of the drainage elastic slice.
Further, it further includes being located in the second chamber to stay liquid raised that the sputum sample, which leaves and takes cup, described
Liquid protrusion is stayed to connect with the cup body.
Further, described to stay liquid protrusion and the liquid outlet facing each other on gravity direction.
Further, described that liquid protrusion is stayed to include the first protrusion, the second protrusion and third protrusion, first protrusion, institute
It states the second protrusion and the third protrusion is connected with each other, and extend respectively along first direction, second direction and third direction, it is described
First direction, the second direction and the third direction are mutually perpendicular to two-by-two；
First protrusion, second protrusion and the third protrusion are equipped with multiple, and each first protrusion is mutually
Interval setting, each second protrusion are spaced apart from each other setting, and each third protrusion is spaced apart from each other setting, each first protrusion
It is connected to the bottom of the cup body.
The present invention also provides a kind of methods that mycobacterium tuberculosis is separated in sputum, include the following steps:
Preparing above-mentioned sputum sample and leaves and takes cup, suction pipe and centrifuge tube, it is ensured that first cover cap seals the accommodating cavity,
And second cover cap seals the liquid outlet, is placed with the sputum sample in the second chamber memory；
Second lid is removed；
4% sodium hydroxide with the sputum sample equivalent is added toward the liquid outlet；
The liquid outlet is sealed with second cover cap；
The sputum sample is left and taken cup to turn upside down mixing；
The sputum sample is left and taken into cup and stands 15-20 minutes under room temperature environment, the sputum sample is made sufficiently to liquefy；
Second lid is removed；
The second chamber is protruded into from the liquid outlet with the suction pipe, and draws the sputum sample after liquefaction；
The sputum sample after liquefaction is injected in the centrifuge tube；
Concussion centrifugation；
Supernatant is abandoned, stays sediment in the centrifuge tube.
Further, supernatant is being abandoned, is staying sediment after the step in the centrifuge tube, further includes following steps:
Add physiological saline in the centrifuge tube；
Concussion centrifugation；
Supernatant is abandoned, stays sediment in the centrifuge tube；
Add lysate in the centrifuge tube；
Concussion centrifugation, and the liquid for forming cracking is located at centrifugation bottom of the tube；
Metal bath 8-12 minutes；
Centrifugation.
Implement the embodiment of the present invention, will have the following beneficial effects:
After leaving and taking cup using above-mentioned sputum sample, when being stored in sputum sample, the first lid can be opened, from cup
The opening of body pours into sputum sample, and sputum sample flows into second chamber under the guiding function of drainage elastic slice, and at this time
The sputum sample of second chamber is stored in because there is the barrier effect of drainage elastic slice without evaporating, can not only be effectively reduced
Infection experiment personnel or a possibility that pollution environment, because drainage elastic slice blocks, experimenter or patient not can be appreciated that the
Existing sputum in two chambers, it is all more friendly to experimenter and patient because of the experience such as nausea without caused by；Moreover, by
Be resisted against block from below in the second end of drainage elastic slice, though turn upside down shake up during, phlegm in second chamber
Liquid sample will not enter first chamber, will not more pollute the first lid or the second lid, it is excessive or dirty to greatly reduce sample
Contaminate the risk of table top；Certainly, when absorption sputum sample leaves and takes the sputum sample in cup, user can not open the first lid
In the case where, suction pipe is passed through into liquid outlet, and support the drainage elastic slice, made to drain between the second end and block of elastic slice between formation
Gap, then suction pipe continues to be passed down through the gap, just can enter and draws sputum sample in second chamber, greatly reduces and sampling
Infection risk in the process.
The method of mycobacterium tuberculosis is separated in sputum provided by the invention due to using above-mentioned sputum sample and leaving and taking cup
Have above-mentioned whole beneficial effects, details are not described herein again.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Wherein:
Fig. 1 is the schematic cross-sectional view that sputum sample leaves and takes cup in one embodiment of the present of invention；
Fig. 2 leaves and takes cup for sputum sample in Fig. 1 and removes the schematic cross-sectional view after the second lid；
Fig. 3 is sectional view along A-A in Fig. 2.
Each appended drawing reference in figure are as follows:
10, sputum sample leaves and takes cup；
100, cup body；110, cavity is accommodated；111, first chamber；112, second chamber；
200, the first lid；210, liquid outlet；
300, the second lid；
400, link block；
500, block；
600, elastic slice is drained；610, first end；620, second end；
700, stay liquid raised；710, the first protrusion；720, the second protrusion；730, third protrusion.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
It should be noted that it can be directly another when element is referred to as " being fixed on " or " being set to " another element
On one element or indirectly on another element.When an element is known as " being connected to " another element, it can
To be directly to another element or be indirectly connected on another element.
It is to be appreciated that term " length ", " width ", "upper", "lower", "front", "rear", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "top", "bottom", "inner", "outside" is that orientation based on the figure or position are closed
System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have
Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more of the features.In the description of the present invention, the meaning of " plurality " is two or more,
Unless otherwise specifically defined.
Sputum sample provided by the invention leaves and takes cup 10, particularly for the preservation and acquisition to sputum, naturally it is also possible to use
In leaving and taking for other sample liquid, unique restriction is not done herein.
Also referring to Fig. 1 to Fig. 3, cup 10 now is left and taken to sputum sample provided by the invention and is illustrated.Sputum sample
Cup 10 is left and taken to include cup body 100, the first lid 200, the second lid 300, link block 400, block 500 and drain elastic slice 600,
Wherein, cup body 100 has accommodating cavity 110, and the first lid 200 is covered on cup body 100, seals the accommodating cavity 110 to cover,
Liquid outlet 210 is offered on first lid 200, which is connected with accommodating cavity 110, and user can take liquid by this
Hole 210 extend into sampling in accommodating cavity 110, and the second lid 300 can cover removably and be enclosed on liquid outlet 210, cover this
It can avoid sputum sample during transportation when two lids 300 to overflow from liquid outlet 210, in this way, the sputum sample leaves and takes cup 10
Meet the condition that also can be carried out sputum sample accessing operation in the case where only opening the second lid 300.
In addition, link block 400 and block 500 are all connected to the inner wall of cup body 100, drainage elastic slice 600 has opposite set
The first end 610 and second end 620 set, the first end 610 of the drainage elastic slice 600 are fixedly connected with link block 400, drain elastic slice
600 second end 620 is resisted against the block 500 from below, and above-mentioned accommodating cavity 110 is divided into first chamber 111 and
Two chambers 112, which is not connected to mutually with second chamber 112 and first chamber 111 is located at the upper of second chamber 112
Side, when sputum sample fall in drainage elastic slice 600 on when, due to drainage elastic slice 600 second end 620 from below with 500 phase of block
It supports, under the gravity of sputum sample, the second end 620 of drainage elastic slice 600 can be detached from block 500, make first chamber 111
It is connected to second chamber 112, sputum sample then can flow to second chamber 112 along drainage elastic slice 600 under the effect of gravity, to phlegm
Liquid sample flow into second chamber 112 after, drainage elastic slice 600 be no longer influenced by the gravity of sputum sample and spring back reset, again with gear
Block 500 is supported, and is connected to first chamber 111 mutually not with second chamber 112.
In this way, the first lid 200 can be opened when being stored in sputum sample, pour into sputum from the opening of cup body 100
Sample, sputum sample flows into second chamber 112 under the guiding function of drainage elastic slice 600, and has been stored in second chamber at this time
It is real can not only to effectively reduce infection because there is the barrier effect of drainage elastic slice 600 without evaporating for 112 sputum sample
A possibility that testing personnel or pollution environment, because drainage elastic slice 600 blocks, experimenter or patient not can be appreciated that the second chamber
Existing sputum in room 112, it is all more friendly to experimenter and patient because of the experience such as nausea without caused by；Moreover, because
Drainage elastic slice 600 second end 620 be resisted against block 500 from below, even if turn upside down shake up during, second chamber
Sputum sample in 112 will not enter first chamber 111, will not more pollute the first lid 200 or the second lid 300, significantly
Reduce sample it is excessive or pollution table top risk；Certainly, when absorption sputum sample leaves and takes the sputum sample in cup 10, user
Suction pipe can be passed through into liquid outlet 210 in the case where not opening the first lid 200, and support the drainage elastic slice 600, make to draw
Gap is formed between the second end 620 and block 500 of stray bullet piece 600, then suction pipe continues to be passed down through the gap, just can enter
Sputum sample is drawn in second chamber 112, greatly reduces the infection risk in sampling process.
In one embodiment, the first lid 200 is detachably connected with cup body 100, is sealed up with lid and is stated accommodating cavity 110.?
In the present embodiment, the first lid 200 is tightly connected by the way of being threadedly engaged with cup body 100, certain first lid 200 and cup
Body 100 can also be connected using modes such as sealing clampings, not do unique restriction herein.
In one embodiment, the second lid 300 is threadedly coupled with the first lid 200, is sealed up with lid and is stated liquid outlet 210,
In the present embodiment, which is preferably rubber parts.Certainly in other embodiments of the invention, 300 He of the second lid
First lid 200 can also be connected using other modes, such as sealing clamping.
In one embodiment, link block 400 and block 500 form difference in height on gravity direction, and link block 400 is relatively kept off
Block 500 is closer to the top of cup body 100.Specifically, on gravity direction, which is located at the top of block 500,
In this way, the first end 610 of drainage elastic slice 600 is located at the top of second end 620, therefore when sputum sample is fallen on sputum sample,
Second end 620 can be flowed to rapidly, to flow to second chamber 112 at faster speed, be further reduced the volatilization of sputum sample, be
To pollution environment or the possibility of infection experiment personnel.
In one embodiment, on gravity direction, link block 400 and block 500 are mutually staggered with liquid outlet.In this way,
It collides when can be moved downward to avoid the suction pipe protruded into from liquid outlet with link block 400 or block 500, to conveniently take
Sample, and help to improve sampling efficiency.
In one embodiment, the oval sheet of elastic slice 600 is drained.The size of the drainage elastic slice 600 is suitable with 100 phase of cup body
Match, first chamber 111 and second chamber 112 can be separated by being subject to, and not do unique restriction herein.Preferably, the drainage bullet
Piece 600 is chosen as rubber parts
In one embodiment, it further includes being located in second chamber 112 to stay liquid protrusion 700 that sputum sample, which leaves and takes cup 10,
Liquid protrusion 700 is stayed to connect with the bottom of cup body 100.This stays liquid protrusion 700 to have fixed make to viscous liquid to a certain extent
With so that sputum sample be made to be not easy reverse flow to 100 opening of cup body.Certainly in other embodiments of the invention, this is stayed
Liquid protrusion 700 can also be arranged on the inner sidewall of cup body 100, not do unique restriction herein.
Further, it includes first the 710, second protrusion 720 of protrusion and third protrusion 730 that this, which stays liquid protrusion 700, and first is convex
It plays the 710, second protrusion 720 and third protrusion 730 is connected with each other, and prolong respectively along first direction, second direction and third direction
It stretches, first direction, second direction and third direction are mutually perpendicular to two-by-two；And first the 710, second protrusion 720 of protrusion and third
Protrusion 730 is equipped with multiple, and each first protrusion 710 is spaced apart from each other setting, and each second protrusion 720 is spaced apart from each other setting, each third
Protrusion 730 is spaced apart from each other setting, and each first protrusion 710 is connected to the bottom of cup body 100.It is wherein a, first direction, second direction
And third direction refers to direction parallel with X-axis in figure, Y-axis and Z axis respectively, in this way, this stays liquid protrusion 700 in three-dimensional framework
Structure can advanced optimize a year old fixed effect for sputum sample.
In one embodiment, on gravity direction, stay liquid protrusion 700 facing each other with liquid outlet.It can make so more
Sputum sample quickly contacted with liquid protrusion 700 is stayed, advanced optimize its fixed effect to sputum sample.
The present invention also provides a kind of methods that mycobacterium tuberculosis is separated in sputum, include the following steps:
Prepare above-mentioned sputum sample and leave and take cup 10, suction pipe and centrifuge tube, and ensures the first lid 200 lid envelope accommodating cavity
110, and the second lid 300 lid envelope liquid outlet 210, it has been stored in second chamber 112 and has acquired the sputum of taking at patient
Sample；Preferably, above-mentioned suction pipe is plastic suction pipe.
Second lid 300 is removed；
4% sodium hydroxide with sputum sample equivalent is added toward liquid outlet 210；
Envelope liquid outlet 210 is covered with the second lid 300；
Sputum sample is left and taken cup 10 to turn upside down mixing；
Sputum sample is left and taken into cup 10 under room temperature environment and stands 15-20 minutes, sputum sample is made sufficiently to liquefy；
Second lid 300 is removed；
Second chamber 112 is protruded into from liquid outlet 210 with suction pipe, and draws the sputum sample after liquefaction；
It will be in the sputum sample injection centrifuge tube after liquefaction；
Concussion centrifugation；Preferably, in this step, with the speed concussion of 13000r/min centrifugation 8-12 minutes, preferably
10 minutes；
Supernatant liquor is abandoned, stays sediment in centrifuge tube.At this point, mycobacterium tuberculosis is located in sediment.
The method that mycobacterium tuberculosis is separated in sputum provided by the invention leaves and takes cup 10 because using above-mentioned sputum sample
And have whole beneficial effects that above-mentioned sputum sample leaves and takes cup, details are not described herein again.
It further, further include walking as follows after above-mentioned " abandoning supernatant, stay sediment in centrifuge tube " the step of
It is rapid:
Add 1-2 milliliters of physiological saline in centrifuge tube；
Concussion centrifugation；Preferably, in this step, with the speed concussion of 13000r/min centrifugation 8-12 minutes, preferably
10 minutes；
Supernatant liquor is abandoned, stays sediment in centrifuge tube；
Add lysate in centrifuge tube；Preferably, in this step, the amount that lysate is added is 50 microlitres；
Concussion centrifugation, and the liquid for forming cracking is located at centrifugation bottom of the tube；
Metal bath 8-12 minutes；
Centrifugation；Preferably, in this step, it is centrifuged 4-6 minutes, preferably 5 minutes with the speed of 13000r/min.Such as
This, that is, obtain the sample of nucleic acid of mycobacterium tuberculosis.
The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly
It encloses, therefore equivalent changes made in accordance with the claims of the present invention, is still within the scope of the present invention.
Claims (10)
1. sputum sample leaves and takes cup characterized by comprising
Cup body has accommodating cavity；
First lid seals the accommodating cavity for covering, offers liquid outlet on first lid；
Second lid, removably lid seals the liquid outlet；
Link block is connected to the cup body, and is located in the accommodating cavity；
Block is connected to the cup body, and is located in the accommodating cavity；And
Elastic slice is drained, the drainage elastic slice has the first end and second end being oppositely arranged, and the first end is connected to the company
Block is connect, the second end is supported with the block from below, and the accommodating cavity is divided into mutual disconnected first chamber
And second chamber, the second end can be under the gravity of the sputum sample far from the block, and by the sputum sample
Second chamber described in this pilot flow direction, the drainage elastic slice can automatic reboundings after the sputum sample flows into the second chamber
It resets.
2. sputum sample as described in claim 1 leaves and takes cup, it is characterised in that: first lid and the cup body are detachable
Connection seals the accommodating cavity with lid.
3. sputum sample as described in claim 1 leaves and takes cup, it is characterised in that: the link block and the block are in gravity side
It is upwardly formed difference in height, and the link block block is closer to the top of the cup body.
4. sputum sample as described in claim 1 leaves and takes cup, it is characterised in that: on gravity direction, the link block and institute
Block is stated to mutually stagger with the liquid outlet.
5. sputum sample as described in claim 1 leaves and takes cup, it is characterised in that: the oval sheet of the drainage elastic slice.
6. as sputum sample described in any one of claim 1 to 5 leaves and takes cup, it is characterised in that: the sputum sample leaves and takes cup
It further include being located in the second chamber to stay liquid raised, it is described that liquid protrusion is stayed to connect with the cup body.
7. sputum sample as claimed in claim 6 leaves and takes cup, it is characterised in that: on gravity direction, it is described stay liquid protrusion with
The liquid outlet face.
8. sputum sample as claimed in claim 6 leaves and takes cup, it is characterised in that: described to stay liquid protrusion include the first protrusion, the
Two protrusions and third protrusion, first protrusion, second protrusion and the third protrusion are connected with each other, and respectively along first
Direction, second direction and third direction extend, and the first direction, the second direction and the third direction mutually hang down two-by-two
Directly；
First protrusion, second protrusion and the third protrusion are equipped with multiple, and each first protrusion is spaced apart from each other
It is arranged and is connected to the bottom of the cup body, each second protrusion is spaced apart from each other setting, and each third protrusion is spaced apart from each other
Setting.
9. separating the method for mycobacterium tuberculosis in sputum, which comprises the steps of:
Prepare sputum sample as claimed in any one of claims 1 to 8 and leave and take cup, suction pipe and centrifuge tube, it is ensured that first lid
Body lid seals the accommodating cavity, and second cover cap seals the liquid outlet, is placed with the phlegm in the second chamber memory
Liquid sample；
Second lid is removed；
4% sodium hydroxide with the sputum sample equivalent is added toward the liquid outlet；
The liquid outlet is sealed with second cover cap；
The sputum sample is left and taken cup to turn upside down mixing；
The sputum sample is left and taken into cup and stands 15-20 minutes under room temperature environment, the sputum sample is made sufficiently to liquefy；
Second lid is removed；
The second chamber is protruded into from the liquid outlet with the suction pipe, and draws the sputum sample after liquefaction；
The sputum sample after liquefaction is injected in the centrifuge tube；
Concussion centrifugation；
Supernatant is abandoned, stays sediment in the centrifuge tube.
10. separating the method for mycobacterium tuberculosis in sputum as claimed in claim 9, which is characterized in that abandoning supernatant, staying
Sediment further includes following steps after the step in the centrifuge tube:
Add physiological saline in the centrifuge tube；
Concussion centrifugation；
Supernatant is abandoned, stays sediment in the centrifuge tube；
Add lysate in the centrifuge tube；
Concussion centrifugation, and the liquid for forming cracking is located at centrifugation bottom of the tube；
Metal bath 8-12 minutes；
Centrifugation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910518508.3A CN110220758A (en) | 2019-06-15 | 2019-06-15 | Sputum sample leaves and takes the method that mycobacterium tuberculosis is separated in cup and sputum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910518508.3A CN110220758A (en) | 2019-06-15 | 2019-06-15 | Sputum sample leaves and takes the method that mycobacterium tuberculosis is separated in cup and sputum |
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Cited By (1)
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| CN114887535A (en) * | 2022-05-23 | 2022-08-12 | 四川省天晟源环保股份有限公司 | Oscillation container and soil pH value determination method |
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