CN209559810U - Micro-fluidic electrochemica biological sensor - Google Patents

Abstract

The utility model is bright to disclose micro-fluidic electrochemica biological sensor, including electrode group and insulator, and electrode group includes working electrode and to electrode, and insulator is equipped at least one detection slot, and electrode group is located in detection slot.The utility model provides one kind can be simultaneously to multiple samples or a kind of micro-fluidic electrochemica biological sensor that many kinds of parameters of sample is detected.

Description

Micro-fluidic electrochemica biological sensor

Technical field

The utility model relates to biochemistry detection fields, more specifically, it is related to micro-fluidic electrochemica biological sensor.

Background technique

In the field of medicine, micro-fluidic electrochemica biological sensor is widely used in analyzing the biological substance including blood, Such as test urine, blood, blood plasma or organize glucose, ketoboidies, cholesterol, lipoprotein, three acid glycerols in the liquid samples such as also Rouge, acetaminophen, Hematocrit and/or HbAlc etc..In this sensor, the micro-fluidic electrochemica biological sensor of enzyme is utilized Measurement sensitivity is high, and can be quickly obtained test result, is commonly applied to the places such as hospital clinic, laboratory or family.

For example, in the electrode system manufactured with silk-screen printing, it is fixed on the electrode by the reagent that will include enzyme, it is added Then sample adds voltage, the interested analyte of quantitative measurment at electrode both ends.United States Patent (USP) US5437999 is draped over one's shoulders Revealed it is a kind of manufacture electrochemica biological sensor calibration tape method, this method using photolithography to be fixed on insulation Conductive material on substrate makes figure, and conductive material is directly printed on to the printed circuit base of standard by silk-screen printing Piece.No. US5437999 also discloses and will separate above added with the insulating substrate of electrode and another insulating substrate as lid Spacer come formed one absorption sample capillary space.

The high production cost of photolithography, and photolithography makes fine figure in a large area The effect of shape is bad, it is difficult to for being mass produced.Silk-screen printing needs the conductive material of liquid phase, such as gold, palladium, platinum inertia gold Belong to, detection performance and chemical corrosion resistance are superior, are suitble to be used as the material of manufacture electrode, but its manufacturing process and technique are all It is very complicated, and they are very expensive.Therefore, the inert metal for all replacing these expensive in practice using carbon.But use silk screen The surface for printing electrode band made from the method for carbon is very uneven, therefore detection performance is bad.There are also one kind pass through by inertia gold Category is deposited on the heavy gauge wire obtained on copper, is bonded on such as substrate of plastic film one kind using the method for heating.This side The shortcomings that method is to be difficult that narrow and thin shape for electrode band is made.And the charge as caused by reacting between reagent and sample is more Close to electrode, these charges are more likely captured and are detected by electrode.In addition, on a plastic film by heavy gauge wire bonding, can make The calibration tape detection efficiency of the biosensor of electrochemistry reduces.

Also, common commercialization electrochemical test bar only one reaction chamber mostly at present, and measurement is only capable of pair every time One-component detected or one bleed or one drop sample liquid can only detect a kind of parameter, be unable to satisfy multisample or more The detection demand of parameter.

Summary of the invention

The purpose of this utility model is to provide a kind of a kind of many kinds of parameters progress that can simultaneously to multiple samples or sample The micro-fluidic electrochemica biological sensor of detection.

The technical scheme adopted by the utility model to solve the technical problem is as follows: micro-fluidic electrochemica biological sensor, packet Include electrode group and insulator, electrode group includes working electrode and to electrode, and insulator is equipped at least one detection slot, electrode group position In in detection slot.

As a preferred option, electrode group has energization area, conducting region and biochemical sensing area, energization area, conducting region and life Change sensing area to be molded in insulator.

As a preferred option, the insulator has obverse and reverse, and front is equipped at least one detection slot, detection slot With adding mouth, all detection slots share an adding mouth or each detection slot is respectively equipped with adding mouth；Reverse side is in adding mouth Corresponding position is provided with groove.As a preferred option, detection slot is separated by separator.Preferably, it is put down between detection slot Row arrangement.Preferably, the depth of each detection slot is identical and of same size.

As a preferred option, adding mouth edge is equipped with protrusion.

As a preferred option, biochemical sensing area is located in detection slot and has the exposed face contacted with substance to be detected.

As a preferred option, each electrode group have one or more working electrode and one or one with On to electrode, each working electrode has a respective lead-out wire, more than one to electrode parallel connection.Preferably, to the life of electrode Change sensing area to be alternately interspersed between the biochemical sensing area of working electrode.Preferably, each electrode group includes five electrodes, Respectively the first working electrode, the second working electrode, third working electrode, a pair of electrodes and second pair of electrode；Five electricity Pole is arranged successively as the first working electrode, a pair of electrodes, second pair of electrode, the second working electrode and third working electrode.Often The electrode number of a electrode group is unlimited.Preferably, the conducting region of a pair of electrodes and second pair of electrode is extended by energization area And it is linked together.Preferably, the n times side that the quantity of electrode group is 2, n=1,2.One time punching molded 8 groups, 16 groups, The even numbers (i.e. 2 n times side, n=1,2) such as 32 groups organize electrode, and speed of production is fast, and yield is high, at low cost.

As a preferred option, detection slot two sides are equipped with the slot for closing with cover plate lid, and the opposite two sidewalls of slot are Miter.

As a preferred option, the surface of insulator has multiple location holes, and location hole is corresponding with electrode position, outside electrode It is exposed in location hole.

As a preferred option, detection rooved face is placed with biochemistry detection piece.

As a preferred option, the both ends of conducting region are connect with energization area and biochemical sensing area respectively；Biochemical sensing area is curved It rolls in conducting region；Energization area and conducting region are generally aligned in the same plane, and biochemical sensing area protrudes from energization area and conducting region.

As a preferred option, energization area plating nickel on surface material.

As a preferred option, electrode group has biochemical sensing area, and biochemical sensing area is successively metal base from inside to outside With inert metal layer.Preferably, biochemical sensing also has dielectric layer, and dielectric layer is between metal base and inert metal layer. Preferably, the material of metal base is silver or copper, and the material of dielectric layer is nickel, and the material of inert metal layer is that palladium, gold or gold close Gold.Preferably, biochemical sensing area is equipped with multiple inert metal test points.Preferably, inert metal test point is 2 or more.It is lazy Property metal there is stronger chemical stability, such as gold, palladium etc..

Utility model has the advantages that

1, sensor is equipped with 1 or more detection slot, can carry out multiple samples or a kind of inspection of sample many kinds of parameters simultaneously It surveys.

2, the biochemical sensing area of electrode group is equipped with 2 or more inert metal test points, it is ensured that has in each detection slot Inert metal test point.

3, electrode group and insulator integrated injection molding, rigidity is strong, good stability, and precision is high.

4, inert metal electrode is molded in insulator, is processed without second, and area dimensionally stable is sensed, thus Induction is accurate.

5, surface metal-layer is followed successively by copper, nickel, inert metal, conduction signal stabilization and surface fine, area from inside to outside With volume accurate stable, so that response error is small.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of the utility model.

Fig. 2 is the structural schematic diagram for testing electrode.

Fig. 3 is the perspective view of sensor.

Fig. 4 is the top view of sensor.

Fig. 5 is the sectional view of Fig. 4 sensor C-C.

Fig. 6 is the schematic diagram of one end face of sensor.

Fig. 7 is the schematic diagram of sensor other end.

It is identified in figure: binder band 1, electrode 2, insulator 3, pre- fault trough 11, working electrode 21, the first working electrode 211, Second working electrode 212, third working electrode 213, to electrode 22, a pair of electrodes 221, second pair of electrode 222, energization area 25, conducting region 26, biochemical sensing area 27, location hole 12, locating plate 13, detection slot 31, the first detection slot 311, the second detection slot 312, separator 32, adding mouth 313, protrusion 3131, groove 314, slot 33, miter 331, cave area 34, pattern number area 35, life Change detection lug 4.

Specific embodiment

The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

As shown in Figure 1, electrochemica biological sensor, including binder band 1, electrode group and insulator 3, electrode group insertion In insulator 3；There are two the connections of binder band 1 and more than two electrode groups, and each electrode group includes at least working electrode 21 A biosensor is formed with to electrode 22, each electrode group and the insulator 3 for wrapping up it.

The production method of micro-fluidic electrochemica biological sensor

Micro-fluidic electrochemica biological sensor production method, comprising the following steps:

(1) substrate is chosen, to substrate punch forming, forms the binder band connected with pre- fault trough and multiple electrodes group；

(2) winding belt package is carried out to binder band and multiple electrodes group；

(3) the energization area surface for cleaning each electrode group carries out nickel plating to energization area surface；

(4) the biochemical sensing area surface for cleaning each electrode group, to biochemical sensing area successively electro-coppering, nickel, inert metal；

(5) surface for cleaning each electrode group, carries out winding belt package to electrode group and binder band again；

(6) by insulator and multiple electrodes group integrated injection molding, multiple sensors are formed.

Test electrode

As shown in Fig. 2, test electrode, including binder band 1 and two or more electrode groups, electrode group include work electricity Pole and to electrode, electrode group is connect with binder band 1 by pre- fault trough 11.In use, the pre- fault trough 11 that fractures separates electrode group With binder band 1.

As shown in Fig. 2, electrode group has energization area 25, pre- fault trough 11 is between energization area 25 and binder band 1.Connection Material strip 1 is equipped with location hole 12.Location hole 12 is positioned for testing electrode group and injection mold.As a specific embodiment party Formula, each electrode group are corresponding with a location hole 12.25 plating nickel on surface material of energization area.Electrode group also has biochemical sensing area 27, biochemical sensing area is successively electroplate with metal base and inert metal floor from inside to outside, and inert metal is inert metal.As One specific embodiment, biochemical sensing area also have dielectric layer, and dielectric layer is between metal base and inert metal layer. Metal base is silver or copper, and the material of dielectric layer is nickel, and the material of inert metal layer is palladium, gold or billon.Currently, inertia is golden Category can not be directly plated on metal base, need nickel as medium, copper and inert metal are combined.With the development of science and technology, can The method that can directly combine metal base and inert metal layer without dielectric layer can be will appear.In electroplating process, inertia Metal layer need it is fully wrapped around live metal base and dielectric layer, specifically, inert metal layer equably wrap metal base and Dielectric layer.It is silver-colored, copper property is unstable this is because metal base is the materials such as silver, copper, if the silver or copper after being oxidized with it is to be checked Substance contact is surveyed, will affect the testing result of substance to be detected.Specifically, inertia gold of the biochemical sensing area 27 equipped with 2 or more Belong to test point.Inert metal has stronger chemical stability, such as gold, palladium etc..It is multiple due to being provided in biochemical sensing area 27 The inert metal test point of detection slot 31, setting 2 or more can be distributed in multiple detection slots 31, it is ensured that each detection slot 31 Inside there is inert metal test point.

As a specific embodiment, the edge of binder band 1 is equipped with locating plate 13, has between each electrode group There is locating plate 13.Locating plate 13 is that convex block or locating plate 13 are folded upward at, when to multiple electrodes group punch forming, positioning Piece 13 is conducive to separate between multiple electrodes group, while having the function of limit, when preventing from carrying out injection molding to electrode group Electrode group and binder band 1 are mobile.

As one embodiment, the quantity of electrode group be the even numbers such as 8 groups, 16 groups, 32 groups (i.e. 2 n times side, n=1, 2) group.One time punching molded multiple electrodes group, speed of production is fast, and yield is high, so that cost greatly reduces.

As shown in Figures 2 and 3, as one embodiment, electrode group further includes conducting region 26, the both ends difference of conducting region 26 It is connect with energization area 25 and biochemical sensing area 27；Biochemical sensing area 27 is bent in conducting region 26.Biochemical sensing area 27 is bent in biography Area 26 is led to be conducive to make detection slot 31 on insulator 3.As a specific embodiment, the biochemical sensing of each electrode group Area 27 is generally aligned in the same plane.

As shown in Fig. 2, conducting region 26 and energization area 25 are generally aligned in the same plane, biochemical sensing area 27 as one embodiment Protrude from conducting region 26 and energization area 25.Insulator 3 uses plastic material.Specifically, insulator 3 uses PBT material.Biochemistry sense Survey that area 27 protrudes from conducting region 26 and energization area 25 is thickness in order to increase plastic wraps and for controlling the depth of detection slot 31 Shallowly, reach most suitably used 31 depth of detection slot.

As a preferred option, each electrode group include one or more working electrode 21 and one or More than one to electrode 22, each working electrode 21 has respective lead-out wire, and multiple pairs of electrodes 22 are in parallel.It is specific as one Embodiment, working electrode 21 and the biochemical sensing area 27 of electrode 22 is alternately arranged, the biochemical sensing area 27 of electrode 22 is worn It inserts between the biochemical sensing area 27 of working electrode 21.Specifically, each electrode group includes five electrodes 2, respectively the One working electrode 211, the second working electrode 212, third working electrode 213, a pair of electrodes 221 and second pair of electrode 222； Five electrodes are arranged successively as the first working electrode 211,221, second pairs of electrodes 222 of a pair of electrodes, the second working electrode 212 With third working electrode 213.The working electrode of the same electrode group and electrode can be combined with each other, for example, the first work electricity Pole 211 and the combination of a pair of electrodes 221 form measure loop, and a pair of electrodes 221 and the combination of the second working electrode 212 form another One measure loop.The electrode number of each electrode group is unlimited, can answer the different number of electricity of electrochemical action different demands collocation Pole.It forms and combines as a whole specifically, the conducting region of a pair of electrodes 221 and second pair of electrode 222 is extended by energization area, referring to Fig. 2.

Micro-fluidic electrochemica biological sensor

As shown in figure 3, micro-fluidic electrochemica biological sensor, including electrode group and insulator 3, insulator 3 are equipped at least 1 A detection slot 31, electrode group are located in detection slot 31.Specifically, detection slot 31 is arranged in parallel, pass through between multiple detection slots 31 Separator 32 is separated, specifically, insulator 3 is opened, there are two detection slots 31, respectively the first detection slot 311 and the second detection Slot 312, referring to fig. 4.A kind of corresponding substance to be detected of each detection slot 31, only one interior electricity that effectively works of each detection slot 31 Pole, effective working electrode refer in each detection slot 31 only have 1 working electrode be covered with enzyme layer, when into detection slot 31 addition to When detecting substance, the working electrode 21 for being covered with enzyme layer constitutes measure loop with to electrode 22.Simultaneously to one or more to be detected When substance is detected, effective working electrode of each detection slot 31 is staggered.

As a specific embodiment, the depth of each detection slot 31 is all the same and width is all the same.Detection slot 31 consistency are good, and error is small between batch.

As shown in figure 3,31 one end of detection slot has adding mouth 313, substance to be detected as a specific embodiment Entered in detection slot 31 by adding mouth 313, all detection slots 31 can share an adding mouth 313 or each detection slot 31 are respectively equipped with adding mouth 313.As shown in figure 3, arrow A show be cholesterol sample loading alternative, i.e., it is straight above detection slot Connect the addition cholesterol substance into detection slot.The insulator 3 has obverse and reverse, detection slot 31 and the equal position of adding mouth 313 In front, reverse side is provided with groove 314 in 313 corresponding position of adding mouth, groove 314 be in order to prevent substance to be detected from adding mouth 313 flow into the reverse side of insulator 3, influence the detection of substance to be detected.As a specific embodiment, 313 side of adding mouth Edge is equipped with protrusion 3131, and protrusion 3131 is that substance to be detected is flowed out from adding mouth 313 in order to prevent.

As a specific embodiment, each electrode group all has energization area 25, conducting region 26 and biochemical sensing area 27, conducting region 26 and biochemical sensing area 27 are molded in insulator 3.Insulator 3 and electrode group are integrated injection molding, Rigidity is strong, good stability, and precision is high.

As a specific embodiment, biochemical sensing area 27 is located in detection slot 31, and biochemical sensing area 27 exposes to Insulator 3.The biochemical sensing area 27 of one electrode group is respectively positioned in detection slot 31.

As a specific embodiment, insulator 3 is set there are two slot 33, and all detection slots 31 are respectively positioned on two and insert Between slot 33, as shown in figure 5,33 two sidewalls of slot are miter 331.When hydrophilic cover plate lid closes detection slot 31,331 side of miter Just slot 33 is bonded with hydrophilic cover plate, and the difference of height that when fitting generates can improve adaptation.Hydrophilic cover plate be soft plastic or its He is suitble to material to be made.

As a specific embodiment, as shown in Figure 6 and Figure 7, the surface of insulator 3 has multiple location holes 12, Location hole 12 is corresponding with electrode group position, and electrode group is exposed in location hole 12.As a specific embodiment, insulator 3 obverse and reverse is equipped with multiple location holes 12 of different sizes.

As a more specifically embodiment, multiple electrodes group can be packed with rolling, it is therefore desirable to which location hole 12 will Electrode group is fixed.The electrode of each electrode group is arranged successively as 221, second pairs of the first working electrode 211, a pair of electrodes electrodes 222, the second working electrode 212 and third working electrode 213.As shown in fig. 6, the front of insulator 3 has 15 location holes, it should End face has detection slot 31.The positive energization area 25 exposes to insulator 3.The conducting region 26 of working electrode 211 is with certain Spacing is vertically equipped with 3 location holes 12.2 location holes 12 are vertically equipped with a fixed spacing to the conducting region 26 of electrode 22.Work The conducting region 26 of electrode 212 is vertically equipped with 2 location holes 12 with a fixed spacing.Wherein, conducting region 26 is laterally in two rows The location hole 12 of line-styled arrangement, wherein row's location hole 12 is hollow out setting, electrode group is located therein, the location hole 12 Size is less than remaining location hole 12.

As shown in fig. 7, the reverse side of insulator 3 has 18 location holes 12.The 2 energization area of electrode of each electrode group is correspondingly Equipped with location hole 12, the location hole 12 in line-styled arrange, location hole 12 be rectangle, be used for injection molding when positioning and Fixed energization area 25.Conducting region 26 is equipped with three row's location holes 12, and every row's location hole 12 is arranged in line-styled.Each electrode 2 Biochemistry detection area 27 is equipped with a location hole 12,12 vertical array of location hole, for positioning biochemistry detection area 27.The end Face is equipped with cave area 34 and pattern number area 35 for label, and cave area 34 is corresponding with 25 position of energization area, pattern number area 35 and conduction 26 position of area is corresponding.

As one embodiment, 31 surface of detection slot is placed with biochemistry detection piece 4, as shown in Figure 3.In use, respectively will Two biochemistry detection pieces 4 are put into corresponding detection slot 31, are carried out subregion placement, are marked on cover plate by color or text Note, one drips detected liquid by being further separated into detection slot 31, passes to signal through electrode after reacting through biochemistry detection piece 4 Check that equipment will inform testing result.

In the case where lacking any element specifically disclosed herein, limitation, may be implemented illustrated and described herein Utility model.Used terms and expressions method is used as the term of explanation rather than limits, and is not intended in these terms Any equivalent of shown and described feature or part thereof with exclusion in the use of representation, and should be realized that various change Type is all feasible in the scope of the utility model.It is therefore to be understood that although passing through various embodiments and optional feature The utility model is specifically disclosed, but the modifications and variations of concept as described herein can be by those of ordinary skill in the art institute Using, and think that these modifications and variations are fallen within the scope of the utility model of the appended claims restriction.

It is described herein or record article, patent, patent application and every other document and can electronically obtain The content of information to a certain extent in full include herein by reference, just as each individual publication by specific and single Solely point out by reference.Applicant retains from any of any this article, patent, patent application or other documents And all material and information are incorporated into the right in the application.

Claims (10)

1. micro-fluidic electrochemica biological sensor, including electrode group and insulator, electrode group includes working electrode and to electrode, It is characterized in that, insulator is equipped at least one detection slot, and electrode group is located in detection slot.

2. micro-fluidic electrochemica biological sensor as described in claim 1, which is characterized in that working electrode has one or one More than, there are one or more to electrode；Multiple pairs of electrode parallel connections, each working electrode have respective lead-out wire.

3. micro-fluidic electrochemica biological sensor as claimed in claim 2, which is characterized in that electrode group has biochemical sensing Area, biochemical sensing area are located in detection slot and have the exposed face contacted with substance to be detected.

4. micro-fluidic electrochemica biological sensor as claimed in claim 3, which is characterized in that pass through separator between detection slot It separates.

5. micro-fluidic electrochemica biological sensor as claimed in claim 4, which is characterized in that detection slot has adding mouth, institute There are the shared adding mouth of detection slot or each detection slot to be respectively equipped with adding mouth；The edge of adding mouth is equipped with protrusion.

6. micro-fluidic electrochemica biological sensor as claimed in claim 5, which is characterized in that the insulator have front and Reverse side, adding mouth are located at front, and reverse side is provided with groove in adding mouth corresponding position.

7. micro-fluidic electrochemica biological sensor as described in claim 1, which is characterized in that detection slot two sides are equipped with slot, Socket sidewalls are miter.

8. micro-fluidic electrochemica biological sensor as described in claim 1, which is characterized in that detection rooved face is placed with biochemistry Detection lug.

9. micro-fluidic electrochemica biological sensor as described in claim 1, which is characterized in that it is multiple fixed that insulator surface has Position hole, location hole is corresponding with electrode group position, and electrode group is exposed in location hole.

10. micro-fluidic electrochemica biological sensor as claimed in claim 3, which is characterized in that electrode group have energization area and Conducting region, the both ends of conducting region are connect with energization area and biochemical sensing area respectively；Biochemical sensing area is bent in conducting region；Energization area It is generally aligned in the same plane with conducting region, biochemical sensing area protrudes from energization area and conducting region.