CN209508284U - The electrical combined stimulating apparatus of multichannel cell culture - Google Patents

Abstract

The utility model relates to technical field of cell culture, and disclose the electrical combined stimulating apparatus of multichannel cell culture, including photoelectricity boost controller, the bottom of the photoelectricity boost controller is fixedly connected with matrix, and the front of the photoelectricity boost controller is fixedly installed with LCD program interface screen.The electrical combined stimulating apparatus of multichannel cell culture, it can be in cell cultivation process by giving electro photoluminescence cell, to prevent its dedifferentiation, electro photoluminescence will keep the rod-shaped form and line shape form of myocyte, at most worked at the same time up to eight channels, each channel can be independently arranged stimulation protocol, efficiency operation can be achieved for mass cell culture, by the way that slotting photosignal output module output signal can be dialled, then cytositimulation plate is connected by 24 needles, cytositimulation plate is placed in Tissue Culture Dish, cytositimulation plate and Tissue Culture Dish can be placed in carbon dioxide incubator together, the work of incubator is not influenced.

Description

The electrical combined stimulating apparatus of multichannel cell culture

Technical field

The utility model relates to technical field of cell culture, specially a kind of electrical combined stimulation dress of multichannel cell culture It sets.

Background technique

Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology, no matter for For entire biotechnology, or one of them biological cloning technology, cell culture is all an essential mistake Journey, the extensive clone of cell culture inherently cell, cell culture technology can by a cell by mass propgation at For simple many cells that are unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture itself It is exactly the clone of cell, a large amount of cell or its metabolite is obtained by cell culture, because biological product is all from cell It gets, it is possible to say that cell culture technology is most crucial and most basic technology in biotechnology.

For cell in conventional incubator long term culture, the external condition generally given is temperature, carbon dioxide, oxygen at present Gas and culture solution, and certain cells such as cardiac muscle cell, Skeletal Muscle Cell and neural class cell excitation multipotential stem cell culture Need more outside stimulus conditions in journey, the combined stimulation of light and electricity is no, and cardiac muscle cell, Skeletal Muscle Cell And multipotency Cardiac Stem Cells etc. during culture by dedifferentiation, its shrinkage is lost, so propose that a kind of multichannel is thin Born of the same parents cultivate electrical combined stimulating apparatus to solve the above problems.

Utility model content

(1) the technical issues of solving

In view of the deficiencies of the prior art, the utility model provides a kind of electrical combined stimulation dress of multichannel cell culture It sets, has the advantages that photoelectricity stimulation capable of being carried out to cell in cell cultivation process, keep the form of cell, solve certain More outsides are needed in cell such as cardiac muscle cell, Skeletal Muscle Cell and neural class cell excitation multipotential stem cell incubation The combined stimulation of incentive condition, light and electricity is no, and cardiac muscle cell, Skeletal Muscle Cell and multipotency Cardiac Stem Cells , by dedifferentiation, its shrinkage problem is lost Deng during culture.

(2) technical solution

Photoelectricity stimulation can be carried out to cell in cell cultivation process for realization is above-mentioned, keep the mesh of the form of cell , the utility model provides the following technical solutions: the electrical combined stimulating apparatus of multichannel cell culture, including photoelectricity stimulation control Device, the bottom of the photoelectricity boost controller are fixedly connected with matrix, and the front of the photoelectricity boost controller is fixedly installed with LCD program interface screen, the front of the LCD program interface screen are fixedly installed with display screen, the LCD program interface screen Front and be located at display screen on the right side of be fixedly installed with manipulation screen, the front of the photoelectricity boost controller and be located at LCD journey It is fixedly installed with switch on the left of sequence control interface, the front of the photoelectricity boost controller and is located at LCD program interface screen Right side photosignal output module is fixedly mounted, the front of the photosignal output module is fixedly connected with optic electric interface, The front of the photoelectricity boost controller and the bottom for being located at photosignal output module are fixedly connected with digital signal output and connect Mouthful, the front of the photoelectricity boost controller and be located at digital signal output interface on the right side of be fixedly connected with digital signal input Interface, the front of the photoelectricity boost controller and be located at digital signal input interface on the right side of be fixedly connected with USB interface, institute It states and is provided with carbon dioxide incubator on the right side of photoelectricity boost controller, the bottom of the carbon dioxide incubator is fixedly connected with Support base, the inside of the carbon dioxide incubator are fixedly connected with partition, and the top movable of the partition is connected with cell training Ware is supported, cytositimulation plate is placed at the top of the Tissue Culture Dish, the bottom of the cytositimulation plate is fixedly connected with carbon electricity Pole.

Preferably, the photoelectricity boost controller is horizontal cuboid, and the quantity of the matrix is four, is located at The quadrangle of photoelectricity boost controller, matrix are rubber block.

Preferably, in photoelectricity boost controller, the display screen can be the LCD program interface screen installation by adhering Light-emitting diode display, the switch are button.

Preferably, the photosignal output module is being fixed on photoelectricity boost controller just by 16 Screws Face, the quantity of the optic electric interface are eight, and optic electric interface is 24 pin electrode interfaces.

Preferably, the digital signal output interface is identical with digital signal input interface, the carbon dioxide incubator For the cuboid being vertically arranged, the front of carbon dioxide incubator is hinged with chamber door, and the quantity of the support base is four, is located at The quadrangle of carbon dioxide incubator bottom.

Preferably, the quantity of the partition is eight, and the distance between eight partitions are equal, and partition is fixed on carbon dioxide On the inner wall of incubator, the left side of the cytositimulation plate is connect by connecting line with optic electric interface.

Preferably, the Tissue Culture Dish is six orifice plate culture dish of standard, and the carbon electrode two are one group, cytositimulation The bottom of plate is fixedly connected with six groups of carbon electrodes.

(3) beneficial effect

Compared with prior art, the utility model provides a kind of electrical combined stimulating apparatus of multichannel cell culture, tool It is standby following the utility model has the advantages that

It is defeated to control eight optic electric interfaces by photoelectricity boost controller for the electrical combined stimulating apparatus of multichannel cell culture Photosignal out applies the stimulation of electricity and light to outside, and the size of photoelectricity stimulation, frequency of stimulation, time-histories all can be by can Programmable controller setting, can be in cell cultivation process by giving electro photoluminescence cell, and to prevent its dedifferentiation, electro photoluminescence will be kept The rod-shaped form and line shape form of myocyte is at most worked at the same time up to eight channels, and each channel can be independently arranged stimulation side Efficiency operation can be achieved for mass cell culture, by that can dial slotting photosignal output module output signal, then in case Cytositimulation plate is connected by 24 needles, cytositimulation plate is placed in Tissue Culture Dish, cytositimulation plate and cell culture Ware can be placed in carbon dioxide incubator together, by the photoelectricity boost controller outside flat wire connection, cultivate chamber door It can directly shut, not influence the work of incubator.

Detailed description of the invention

FIG. 1 is a schematic structural view of the utility model.

Fig. 2 is the utility model photoelectricity boost controller structural schematic diagram.

Fig. 3 is the utility model carbon dioxide incubator structural schematic diagram.

In figure: 1 photoelectricity boost controller, 2 matrixs, 3 LCD program interface screens, 4 display screens, 5 manipulation screens, 6 switches, 7 Photosignal output module, 8 optic electric interfaces, 9 digital signal output interfaces, 10 digital signal input interfaces, 11 USB interfaces, 12 Carbon dioxide incubator, 13 support bases, 14 partitions, 15 Tissue Culture Dish, 16 carbon electrodes, 17 cytositimulation plates.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.

Referring to Fig. 1, the electrical combined stimulating apparatus of multichannel cell culture, including photoelectricity boost controller 1, photoelectricity stimulation The bottom of controller 1 is fixedly connected with matrix 2, and photoelectricity boost controller 1 is horizontal cuboid, and the quantity of matrix 2 is four, It is located at the quadrangle of photoelectricity boost controller 1, matrix 2 is rubber block, and the front of photoelectricity boost controller 1 is fixedly installed with LCD program interface screen 3, the front of LCD program interface screen 3 are fixedly installed with display screen 4, and LCD program interface screen 3 is just Face and be located at display screen 4 right side be fixedly installed with manipulation screen 5, the front of photoelectricity boost controller 1 and be located at LCD process control The left side at interface 3 is fixedly installed with switch 6, and 3 installation by adhering of LCD program interface screen is in photoelectricity boost controller 1, display Screen 4 can be light-emitting diode display, and switch 6 is button, the front of photoelectricity boost controller 1 and the right side for being located at LCD program interface screen 3 Photosignal output module 7 is fixedly mounted in side, and the front of photosignal output module 7 is fixedly connected with optic electric interface 8, optical telecommunications Number output module 7 is fixed on the front of photoelectricity boost controller 1 by 16 Screws, and the quantity of optic electric interface 8 is eight, Optic electric interface 8 is 24 pin electrode interfaces, the front of photoelectricity boost controller 1 and the bottom for being located at photosignal output module 7 Portion is fixedly connected with digital signal output interface 9, the front of photoelectricity boost controller 1 and is located at digital signal output interface 9 Right side is fixedly connected with digital signal input interface 10, the front of photoelectricity boost controller 1 and is located at digital signal input interface 10 right side is fixedly connected with usb 11, and the right side of photoelectricity boost controller 1 is provided with carbon dioxide incubator 12, dioxy The bottom for changing carbon incubator 12 is fixedly connected with support base 13,10 phase of digital signal output interface 9 and digital signal input interface Together, carbon dioxide incubator 12 is the cuboid being vertically arranged, and the front of carbon dioxide incubator 12 is hinged with chamber door, support base 13 Quantity is four, is located at the quadrangle of 12 bottom of carbon dioxide incubator, the inside of carbon dioxide incubator 12 is fixedly connected There is partition 14, the top movable of partition 14 is connected with Tissue Culture Dish 15, and the top of Tissue Culture Dish 15 is placed with cytositimulation Plate 17, the quantity of partition 14 are eight, and the distance between eight partitions 14 are equal, and partition 14 is fixed on carbon dioxide incubator 12 Inner wall on, the left side of cytositimulation plate 17 connect by connecting line with optic electric interface 8, the bottom fixation company of cytositimulation plate 17 It is connected to carbon electrode 16, Tissue Culture Dish 15 is six orifice plate culture dish of standard, and carbon electrode 16 two are one group, cytositimulation plate 17 Bottom is fixedly connected with six groups of carbon electrodes 16.

In conclusion the electrical combined stimulating apparatus of multichannel cell culture, controls eight by photoelectricity boost controller 1 8 output photoelectric signal of optic electric interface applies the stimulation of electricity and light, the size of photoelectricity stimulation, frequency of stimulation, time-histories to outside It can be all arranged by programmable controller, can be in cell cultivation process by giving electro photoluminescence cell, to prevent its dedifferentiation, electricity Stimulation will keep the rod-shaped form and line shape form of myocyte, at most work at the same time up to eight channels, each channel can be independent Stimulation protocol is set, efficiency operation can be achieved for mass cell culture, it is defeated by the way that slotting photosignal output module 7 can be dialled Then signal out connects cytositimulation plate 17 by 24 needles, cytositimulation plate 17 is placed in Tissue Culture Dish 15, cell Stimulus board 17 and Tissue Culture Dish 15 can be placed in carbon dioxide incubator together, by the photoelectricity outside flat wire connection Boost controller 1, culture chamber door can directly be shut, and not influence the work of incubator.

It should be noted that the terms "include", "comprise" or its any other variant are intended to the packet of nonexcludability Contain, so that the process, method, article or equipment for including a series of elements not only includes those elements, but also including Other elements that are not explicitly listed, or further include for elements inherent to such a process, method, article, or device. In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that including the element Process, method, article or equipment in there is also other identical elements.

While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (7)

1. the electrical combined stimulating apparatus of multichannel cell culture, including photoelectricity boost controller (1), it is characterised in that: the light The bottom of electric-stimulation controller (1) is fixedly connected with matrix (2), and the front of the photoelectricity boost controller (1) is fixedly installed with The front of LCD program interface screen (3), the LCD program interface screen (3) is fixedly installed with display screen (4), the LCD journey The front of sequence control interface (3) and it is located on the right side of display screen (4) and is fixedly installed with manipulation screen (5), photoelectricity stimulation control The front of device (1) and be located at LCD program interface screen (3) on the left of be fixedly installed with switch (6), the photoelectricity boost controller (1) front and photosignal output module (7) are fixedly mounted in the right side for being located at LCD program interface screen (3), the optical telecommunications The front of number output module (7) is fixedly connected with optic electric interface (8), the front of the photoelectricity boost controller (1) and is located at light The bottom of electric signal output module (7) is fixedly connected with digital signal output interface (9), the photoelectricity boost controller (1) Front and being located on the right side of digital signal output interface (9) is fixedly connected with digital signal input interface (10), photoelectricity thorn Swash the front of controller (1) and be located on the right side of digital signal input interface (10) and is fixedly connected with USB interface (11), the light It is provided on the right side of electric-stimulation controller (1) carbon dioxide incubator (12), the bottom of the carbon dioxide incubator (12) is solid Surely it is connected with support base (13), the inside of the carbon dioxide incubator (12) is fixedly connected with partition (14), the partition (14) top movable is connected with Tissue Culture Dish (15), is placed with cytositimulation plate at the top of the Tissue Culture Dish (15) (17), the bottom of the cytositimulation plate (17) is fixedly connected with carbon electrode (16).

2. the electrical combined stimulating apparatus of multichannel cell culture according to claim 1, it is characterised in that: the photoelectricity thorn Swashing controller (1) is horizontal cuboid, and the quantity of the matrix (2) is four, is located at photoelectricity boost controller (1) Quadrangle, matrix (2) are rubber block.

3. the electrical combined stimulating apparatus of multichannel cell culture according to claim 1, it is characterised in that: the LCD journey For sequence control interface (3) installation by adhering in photoelectricity boost controller (1), the display screen (4) can be light-emitting diode display, described Switching (6) is button.

4. the electrical combined stimulating apparatus of multichannel cell culture according to claim 1, it is characterised in that: the optical telecommunications Number output module (7) is fixed on the front of photoelectricity boost controller (1) by 16 Screws, the optic electric interface (8) Quantity is eight, and optic electric interface (8) is 24 pin electrode interfaces.

5. the electrical combined stimulating apparatus of multichannel cell culture according to claim 1, it is characterised in that: the number letter Number output interface (9) and digital signal input interface (10) are identical, and the carbon dioxide incubator (12) is the cuboid being vertically arranged, The front of carbon dioxide incubator (12) is hinged with chamber door, and the quantity of the support base (13) is four, is located at titanium dioxide The quadrangle of carbon incubator (12) bottom.

6. the electrical combined stimulating apparatus of multichannel cell culture according to claim 1, it is characterised in that: the partition (14) quantity is eight, and the distance between eight partitions (14) are equal, and partition (14) is fixed on carbon dioxide incubator (12) Inner wall on, the left side of the cytositimulation plate (17) is connect by connecting line with optic electric interface (8).

7. the electrical combined stimulating apparatus of multichannel cell culture according to claim 1, it is characterised in that: the cell training Supporting ware (15) is six orifice plate culture dish of standard, and the carbon electrode (16) two are one group, and the bottom of cytositimulation plate (17) is fixed It is connected with six groups of carbon electrodes (16).