CN209433116U - Slicer cell and inverted microscope observation system and Patch Clamp System - Google Patents
Slicer cell and inverted microscope observation system and Patch Clamp System Download PDFInfo
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- CN209433116U CN209433116U CN201920050761.6U CN201920050761U CN209433116U CN 209433116 U CN209433116 U CN 209433116U CN 201920050761 U CN201920050761 U CN 201920050761U CN 209433116 U CN209433116 U CN 209433116U
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Abstract
The utility model provides a kind of slicer cell and inverted microscope observation system and Patch Clamp System, it is related to histotomy observation device technical field, slicer cell includes the lead channel being sequentially communicated, it is sliced placing groove and imbibition slot, the bottom of slice placing groove is provided with peephole, creep plate is fixed on peephole, creep plate is for placing slicer, it improves traditional slicer record and imaging will use the vessel or glass slide of ordinary cells culture, perfusion or not perfusion will affect the technical issues of record and imaging effect, slicer cell provided by the utility model is placed in slicer on the creep plate in slice placing groove, slicer can be recorded and be imaged during continuous perfusion, it effectively prevent perfusion liquid to the disturbance of slicer and blotting for liquid, add simultaneously It is also easy to clean after medicine, ensure that the record and imaging effect of slicer.
Description
Technical field
The utility model relates to histotomy observation device fields, more particularly, to a kind of slicer cell and inversion
Microscopic system and Patch Clamp System.
Background technique
Compared to the culture and living animal of cell in vitro, slicer especially acute organ slice has its special
Advantage, it had not only been able to maintain the characteristic in cell body, but also operated and study all relatively easy than living animal.So living tissue is cut
The external record of piece and the research that the related fieldss such as drug toxicology, pharmaceutical research and ion-channel switch in vitro are imaged have
Very important effect.
In the external record and imaging process of slicer, especially nervous system, such as slice of brain are carried out
During electrophysiological recording and Calcium imaging, generally requires and extracellular fluid perfusion is carried out to slice, guarantee the activity of cell, together
When can also use various agonists, inhibitor or various drugs during the experiment, sample can be also added to by way of perfusion
In product.
Traditional slicer record and imaging will use the vessel or glass slide of ordinary cells culture, record in slice
In imaging process, perfusion not can guarantee the activity of cell and the homogeneity of dosing, and perfusion system is added and is easy to disturb again to cut
Piece blots liquid, while being also not easy to thoroughly wash after dosing, influences the effect for recording and being imaged.
In view of this, special propose the utility model.
Utility model content
One of the purpose of this utility model is to provide a kind of slicer cell, to improve traditional slicer note
Record and imaging will use the vessel or glass slide of ordinary cells culture, and perfusion or not perfusion will affect record and imaging effect
Technical problem.
Slicer cell provided by the utility model, including lead channel, slice placing groove and the imbibition being sequentially communicated
The bottom of slot, the slice placing groove is provided with peephole, is fixed with creep plate on the peephole, the creep plate for placing or
Histotomy.
Further, the slicer cell further includes sealing rubber ring, and the creep plate is solid by the sealing rubber ring
Due on the peephole.
Further, the bottom of the lead channel and the bottom of the imbibition slot are above the bottom of the slice placing groove
Portion.
Further, the bottom of the lead channel is higher than the bottom 1-10mm of the slice placing groove, the imbibition slot
Bottom is higher than the bottom 1-10mm of the slice placing groove.
Further, it is provided with feed liquor fixing pipe in the lead channel, imbibition fixing pipe, institute are provided in the imbibition slot
Feed liquor fixing pipe is stated for fixing inlet tube, the imbibition fixing pipe is for fixing pipette.
Further, the diameter of the feed liquor fixing pipe is 3-10mm, and the height of the feed liquor fixing pipe is 5-20mm.
Further, the cross section of the slice placing groove is round or rectangular.
Further, the depth of the lead channel is 4-15mm, and the depth of the imbibition slot is 4-15mm, and it is described into
The bottom of liquid bath is flushed with the bottom of the imbibition slot.
The two of the purpose of this utility model are to provide a kind of inverted microscope observation system, including the utility model provides
Slicer cell and inverted microscope, the inverted microscope is provided with object lens, and the object lens and the peephole connect
It connects.
The three of the purpose of this utility model are to provide a kind of Patch Clamp System, including provided by the utility model be inverted shows
Micro mirror observation system.
Lead channel, slice placing groove and the suction that slicer cell provided by the utility model is sequentially communicated by setting
Liquid bath, slicer are placed on the creep plate in slice placing groove, and during carrying out perfusion, liquid is flowed into from lead channel
It is sliced placing groove, then liquid is sucked out by imbibition slot, slicer is remembered during continuous perfusion
Record and imaging, effectively prevent perfusion liquid to the disturbance of slicer and blotting for liquid, while being also beneficial to be perfused
The mixing and subsequent cleaning removal of drug are added in the process, so that the record and imaging effect of slicer be effectively ensured
Fruit.
In addition, slicer cell provided by the utility model, not only can requirement selection according to specific experiments not
The creep plate of stack pile and optical property, and the replacement of creep plate can be carried out at any time during the experiment, to improve imaging clearly
Degree, while being updated by way of replace creep plate and the cost of cell is imaged also is far below common culture vessel with glass bottom and is replaced with
This, to effectively increase the ease of use of slicer cell, has expanded its application range.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the structural schematic diagram for the slicer cell that the utility model embodiment 1 provides；
Fig. 2 is the decomposition texture schematic diagram in slicer cell shown in Fig. 1；
Fig. 3 is the top view of slicer cell shown in Fig. 1；
Fig. 4 is slicer cell sectional view along A-A shown in Fig. 3.
Icon: 101- lead channel；102- is sliced placing groove；103- imbibition slot；104- peephole；105- creep plate；106- is close
Sealing rubber ring；107- feed liquor fixing pipe；108- imbibition fixing pipe.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical
Novel protected range.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
The concrete meaning of language in the present invention.
The embodiments of the present invention are described in detail below in conjunction with embodiment, but those skilled in the art
It will be understood that the following example is merely to illustrate the utility model, and it is not construed as limitation the scope of the utility model.Embodiment
In the person that is not specified actual conditions, carry out according to conventional conditions or manufacturer's recommended conditions.Life is not specified in agents useful for same or instrument
Manufacturer person is produced, is the conventional products that can be obtained by commercially available purchase.
Embodiment 1
Fig. 1 is the structural schematic diagram for the slicer cell that the utility model embodiment 1 provides；Fig. 2 is living shown in Fig. 1
Decomposition texture schematic diagram in histotomy cell；Fig. 3 is the top view of slicer cell shown in Fig. 1；Fig. 4 is Fig. 3 institute
Show slicer cell sectional view along A-A；As shown in Figs 1-4, slicer cell provided in this embodiment includes successively
Lead channel 101, slice placing groove 102 and the imbibition slot 103 of connection, the bottom of slice placing groove 102 are provided with peephole 104,
Creep plate 105 is fixed on peephole 104.
Slicer cell provided in this embodiment by slice placing groove 102 bottom be provided with peephole 104,
So that the object lens of inverted microscope can by the slicer placed in 104 observed slice placing groove 102 of peephole, from
And it is recorded and is imaged；By fixing creep plate 105 on peephole 104, on the one hand avoid the liquid of perfusion from peephole 104
On the other hand water clock is also conducive to for slicer being placed on creep plate 105, to be more conducive to carry out through 104 He of peephole
Creep plate 105 carries out record and the imaging of slicer.Lead channel 101 with inlet tube for being connected, so that perfusion liquid is first
Imbibition slot 103 is flowed into again by lead channel 101, and imbibition slot 103 is used to hold the liquid that slice placing groove 102 flows out, and imbibition
Slot 103 liquid for being sliced the inflow imbibition slot 103 of placing groove 102 is discharged, for being connected with pipette so that perfusion
Process can continue to carry out, and avoid perfusion liquid from overflowing, damage microscope, cause environmental pollution.
Slicer cell provided by the embodiment of the utility model is by being arranged the lead channel 101 being sequentially communicated, slice
Placing groove 102 and imbibition slot 103, slicer are placed on the creep plate 105 in slice placing groove 102, are carrying out perfusion
In the process, liquid flows into slice placing groove 102 from lead channel 101, then liquid is sucked out by imbibition slot 103, effectively prevents
Perfusion liquid to the disturbance of slicer and blotting for liquid is also beneficial to that the mixing of drug is added in filling process
It is removed with subsequent cleaning, to ensure that the record and imaging effect of slicer.
In a kind of preferred embodiment of the present embodiment, creep plate 105 is sterile creep plate 105, and 105 surface of creep plate is preparatory
The processing of the coatings such as poly-D-lysine or poly-ornithine, laminin or collagen is carried out, to promote slicer
Adherency improves stability of slicer during liquid is perfused, to improve the stability of record and imaging.
In a kind of preferred embodiment of the present embodiment, card slot (not shown) is provided on the hole wall of peephole 104, with
It convenient for creep plate 105 to be fixed in card slot, while preventing creep plate 105 from moving under the action of liquid is perfused, influences group living
Knit the record and imaging effect of slice.
As shown in Fig. 2, slicer cell further includes sealing rubber ring in a kind of preferred embodiment of the present embodiment
106, creep plate 105 is fixed on peephole 104 by sealing rubber ring 106.
Sealing rubber ring 106 is used to creep plate 105 being sealingly fastened in peephole 104, so as to effectively avoid due to climbing
There are gap between 105 edge of piece and peephole 104, caused by liquid drip leakage is perfused.In addition, passing through setting sealing rubber ring 106
Creep plate 105 can also be avoided to disturb during pouring liquid, influence the record and imaging effect of slicer.
In a kind of preferred embodiment of the present embodiment, the bottom of lead channel 101 and the bottom of imbibition slot 103 are high
In the bottom of slice placing groove 102.
By setting the bottom of the bottom of lead channel 101 and imbibition slot 103 to be above the bottom of slice placing groove 102
Portion, so that perfusion liquid flows into after slice placing groove 102 from lead channel 101 and flows into imbibition slot 103 from slice placing groove 102
When liquid flow velocity it is more steady, effectively avoid vortex or turbulent flow appearance, so as to avoid perfusion liquid to slicer
It causes to disturb, further improves the stability of record and imaging.
In a kind of preferred embodiment of the present embodiment, the bottom of lead channel 101 is higher than the bottom of slice placing groove 102
1-10mm, the bottom of imbibition slot 103 are higher than the bottom 1-10mm of slice placing groove 102.
In the preferred embodiment of the present embodiment, slightly above slice is placed for the bottom of lead channel 101 and imbibition slot 103
The bottom of slot 102 can either avoid perfusion liquid from forming turbulent flow or vortex in slice placing groove 102, and can guarantee to be sliced
Liquid in placing groove 102 can be blotted with the liquid in placing groove 102 that replace and be sliced of filling process.Test of many times
It proves, when the bottom of the bottom of lead channel 101 and imbibition slot 103 is above the bottom 1-10mm of slice placing groove 102, both
It can guarantee that the flow rate of liquid for entering slice placing groove 102 is steady, and can guarantee that the liquid in slice placing groove 102 is able to carry out
Replacement in time, to be effectively ensured, slicer is recorded and what is be imaged goes on smoothly.
It is typical but non-limiting, the difference in height for example 1mm of the bottom of lead channel 101 and slice 102 bottom of placing groove,
2mm, 3mm, 4mm, 5mm, 6mm, 7mm, 8mm, 9mm or 10mm.
It is typical but non-limiting, the difference in height for example 1mm of the bottom of imbibition slot 103 and slice 102 bottom of placing groove,
2mm, 3mm, 4mm, 5mm, 6mm, 7mm, 8mm, 9mm or 10mm.
As shown in Figs 1-4, in a kind of preferred embodiment of the present embodiment, feed liquor is provided in lead channel 101 and is fixed
Pipe 107 is provided with imbibition fixing pipe 108 in imbibition slot 103, and feed liquor fixing pipe 107 is for fixing inlet tube, imbibition fixing pipe
108 for fixing pipette.
By the way that feed liquor fixing pipe 107 is arranged in lead channel 101, so that inlet tube can be by being inserted into feed liquor fixing pipe
107 and be fixed in lead channel 101, by imbibition slot 103 be arranged imbibition fixing pipe 108 so that pipette can lead to
It crosses in insertion imbibition fixing pipe 108 and is fixed in imbibition slot 103, to avoid because inlet tube and/or pipette are fixed unstable
Perfusion liquid perturbation caused by and blots, so that the stability of slicer record and imaging be effectively ensured.
In a kind of preferred embodiment of the present embodiment, feed liquor fixing pipe 107 and imbibition fixing pipe 108 set for opposite side
It sets, to guarantee that perfusion liquid is able to carry out abundant exchange in filling process.Such as when 103 side of being of lead channel 101 and imbibition slot
When shape slot, feed liquor fixing pipe 107 is set to the side of lead channel 101, then imbibition fixing pipe 108 is then set to imbibition slot 103
In the other side opposite with the side of lead channel 101.
In a kind of preferred embodiment of the present embodiment, it is highly 5- that the diameter of feed liquor fixing pipe 107, which is 3-10mm,
20mm。
In the preferred embodiment of the present embodiment, the diameter of feed liquor fixing pipe 107 is slightly coarser than the diameter of inlet tube, so as to
It is fixed in inlet tube to be inserted into feed liquor fixing pipe 107；The height of inlet tube is slightly above the depth of lead channel 101, with benefit
In the insertion of inlet tube.
It is typical but non-limiting, the diameter of feed liquor fixing pipe 107 for example 3mm, 4mm, 5mm, 6mm, 7mm, 8mm, 9mm
Or 10mm；Height for example 5mm, 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm, 15mm, 16mm, 17mm,
18mm, 19mm or 20mm.
In a kind of preferred embodiment of the present embodiment, it is highly 5- that the diameter of imbibition fixing pipe 108, which is 3-10mm,
20mm。
In the preferred embodiment of the present embodiment, the diameter of imbibition fixing pipe 108 is slightly coarser than the diameter of pipette, so as to
It is fixed in pipette to be inserted into imbibition fixing pipe 108；The height of pipette is slightly above the depth of imbibition slot 103, with benefit
In the insertion of pipette.
It is typical but non-limiting, the diameter of imbibition fixing pipe 108 for example 3mm, 4mm, 5mm, 6mm, 7mm, 8mm, 9mm
Or 10mm；Height for example 5mm, 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm, 15mm, 16mm, 17mm,
18mm, 19mm or 20mm.
In a kind of preferred embodiment of the present embodiment, the cross section of slice placing groove 102 is circle or rectangular, that is, is cut
Piece placing groove 102 is circular trough or square groove.
Slice placing groove 102 is circular trough or square groove in order to place creep plate 105 and slicer.When slice is placed
When slot 102 is circular trough, a diameter of 5-35mm, when being sliced placing groove 102 is square groove, its side length is 5-35mm, with benefit
In the placement of creep plate 105 and slicer.
It is typical but non-limiting, when slice placing groove 102 is circular trough, the diameter of circular trough for example 5mm, 8mm,
10mm, 12mm, 15mm, 18mm, 20mm, 22mm, 25mm, 28mm, 30mm, 32mm or 35mm.
It is typical but non-limiting, when slice placing groove 102 is square groove, the side length for example 5mm of slice placing groove 102,
8mm, 10mm, 12mm, 15mm, 18mm, 20mm, 22mm, 25mm, 28mm, 30mm, 32mm or 35mm.
In a kind of preferred embodiment of the present embodiment, the depth of lead channel 101 is 4-15mm, the depth of imbibition slot 103
Degree is 4-15mm, and the depth of imbibition slot 103 is 4-15mm, and the bottom of lead channel 101 is flushed with the bottom of imbibition slot 103.
By controlling the depth of lead channel 101 and the depth of imbibition slot 103, it is added in slice placing groove 102 with control
Perfusion liquid total amount, with its can satisfy slicer perfusion needs.By the bottom and the imbibition that control lead channel 101
The bottom of slot 103 flushes, so that perfusion liquid flows into behind cell through slice placing groove 102 via lead channel 101 again from suction
Liquid bath 103 is smoothly discharged, and guarantees that the liquid in slice placing groove 102 can be exchanged sufficiently.
It is typical but non-limiting, the depth of lead channel 101 for example 4mm, 5mm, 6mm, 7mm, 8mm, 9mm, 10mm,
11mm, 12mm, 13mm, 14mm or 15mm.
It is typical but non-limiting, the depth of imbibition slot 103 for example 4mm, 5mm, 6mm, 7mm, 8mm, 9mm, 10mm,
11mm, 12mm, 13mm, 14mm or 15mm.
In a kind of preferred embodiment of the present embodiment, the material of slicer cell is stainless steel, titanium alloy, modeling
Material or glass.
By using stainless steel, titanium alloy, plastics or glass system at slicer cell, it is made to be more conducive to clean and disappear
Poison realizes recycling, saves testing cost.
In a kind of preferred embodiment of the present embodiment, the volume-tunable for being sliced placing groove 102 is whole for 100mm3Left and right,
In favor of the stimulation and cleaning of drug (including inhibitor and activator).In the external record and imaging process of slicer
In, the stimulation of various drugs or agonist and inhibitor is often used, this is just needed using perfusion system perfusion buffer
It is replaced as drug or agonist and inhibitor solution, small-sized slice holding chamber is not only advantageous to both quickly and thoroughly change liquid,
The usage amount of drug, save the cost can be greatlyd save.
Typical but non-limiting, the application method of slicer cell provided in this embodiment is as follows: by creep plate 105
It is placed on the peephole 104 of slice 102 bottom of placing groove, and creep plate 105 and peephole 104 is sealed using sealing rubber ring 106
Between gap so that creep plate 105 is fixed on peephole 104, then by inlet tube by pouring liquid to lead channel 101
In, perfusion liquid flows into slice placing groove 102 by lead channel 101, with the continuous injection of perfusion liquid, is sliced placing groove
Liquid is increasing in 102, is flowed into imbibition slot 103, and the liquid in imbibition slot 103 is discharged by pipette again, guarantees perfusion
The lasting progress of process.
It should be noted that slicer cell provided in this embodiment is also applied for record and the imaging of living cells,
When the record for living cells and when imaging, by living cells culture on creep plate.
Embodiment 2
A kind of inverted microscope observation system is present embodiments provided, the work provided including inverted microscope and embodiment 1
Histotomy cell, wherein inverted microscope includes object lens, and object lens are connect with peephole 104, to observe slicer in real time
Change procedure, to be recorded and be imaged more acurrate clearly data.
Embodiment 3
A kind of Patch Clamp System is present embodiments provided, it is described including the inverted microscope observation system that embodiment 2 provides
Patch Clamp System includes reference electrode and recording electrode, and the reference electrode is used to be placed on the perfusion in slice placing groove 102
In liquid, recording electrode is for being placed on slicer, to record the signals such as field potential, film potential and full cell currents.
Multiple Patch Clamp System test experience is the results show that lead channel, slice placing groove and imbibition slot are sequentially communicated setting
Effectively prevent the interference that perfusion records small electrical signals, for dosing test, the lesser slice placing groove of preferred volume with
Into, imbibition slot, to improve the speed of drug load and removal while having saved drug.In addition it is possible to effectively improve
Patch-clamp techniques success rate reduces signal-to-noise ratio.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should
Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of
Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new
The range of each embodiment technical solution of type.
Claims (10)
1. a kind of slicer cell, which is characterized in that including be sequentially communicated lead channel, slice placing groove and imbibition slot,
The bottom of the slice placing groove is provided with peephole, creep plate is fixed on the peephole, the creep plate is for placing living group
Knit slice.
2. slicer cell according to claim 1, which is characterized in that further include sealing rubber ring, the creep plate is logical
The sealing rubber ring is crossed to be fixed on the peephole.
3. slicer cell according to claim 1, which is characterized in that the bottom of the lead channel and the imbibition
The bottom of slot is above the bottom of the slice placing groove.
4. slicer cell according to claim 1, which is characterized in that the bottom of the lead channel is higher than described cut
The bottom 1-10mm of piece placing groove, the bottom of the imbibition slot are higher than the bottom 1-10mm of the slice placing groove.
5. slicer cell according to claim 1, which is characterized in that be provided with feed liquor in the lead channel and fix
It manages, imbibition fixing pipe is provided in the imbibition slot, for the feed liquor fixing pipe for fixing inlet tube, the imbibition fixation is effective
In fixed pipette.
6. slicer cell according to claim 5, which is characterized in that the diameter of the feed liquor fixing pipe is 3-
10mm, the height of the feed liquor fixing pipe are 5-20mm.
7. slicer cell according to claim 1, which is characterized in that the cross section of the slice placing groove is circle
Shape is rectangular.
8. slicer cell according to claim 1-7, which is characterized in that the depth of the lead channel is
The depth of 4-15mm, the imbibition slot are 4-15mm, and the bottom of the lead channel is flushed with the bottom of the imbibition slot.
9. a kind of inverted microscope observation system, which is characterized in that including the described in any item slicers of claim 1-8
Cell and inverted microscope, the inverted microscope are provided with object lens, and the object lens are connect with the peephole.
10. a kind of Patch Clamp System, which is characterized in that including inverted microscope observation system as claimed in claim 9.
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| CN201920050761.6U CN209433116U (en) | 2019-01-11 | 2019-01-11 | Slicer cell and inverted microscope observation system and Patch Clamp System |
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| CN201920050761.6U CN209433116U (en) | 2019-01-11 | 2019-01-11 | Slicer cell and inverted microscope observation system and Patch Clamp System |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112056306A (en) * | 2020-06-22 | 2020-12-11 | 南京易太斯生物科技有限公司 | Brain slice perfusion tank suitable for electrophysiological experiments |
| CN113589515A (en) * | 2021-08-19 | 2021-11-02 | 绍兴格物光学有限公司 | Portable microscope observation set |
| CN113589508A (en) * | 2021-08-19 | 2021-11-02 | 绍兴格物光学有限公司 | Image acquisition method based on portable microscope |
-
2019
- 2019-01-11 CN CN201920050761.6U patent/CN209433116U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112056306A (en) * | 2020-06-22 | 2020-12-11 | 南京易太斯生物科技有限公司 | Brain slice perfusion tank suitable for electrophysiological experiments |
| CN113589515A (en) * | 2021-08-19 | 2021-11-02 | 绍兴格物光学有限公司 | Portable microscope observation set |
| CN113589508A (en) * | 2021-08-19 | 2021-11-02 | 绍兴格物光学有限公司 | Image acquisition method based on portable microscope |
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