CN209333776U - A kind of combined micro-fluidic chip nested arrangement - Google Patents
A kind of combined micro-fluidic chip nested arrangement Download PDFInfo
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- CN209333776U CN209333776U CN201822024226.1U CN201822024226U CN209333776U CN 209333776 U CN209333776 U CN 209333776U CN 201822024226 U CN201822024226 U CN 201822024226U CN 209333776 U CN209333776 U CN 209333776U
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Abstract
The utility model provides a kind of combined micro-fluidic chip nested arrangement, including pedestal and the upper cover being fixedly arranged on pedestal, the side wall of pedestal is equipped at least two nested channels, the top of nested channels communicates with the outside world, the upper surface of upper cover is equipped with a sample-adding groove, upper cover offers the aggregation pond of several perforation upper covers, assemble pond behind the outside of sample-adding groove and the sample-adding end insertion nested channels of micro-fluidic chip, aggregation pond is located at the surface of the well of micro-fluidic chip, is loaded between groove and aggregation pond and is communicated with separating liquid channel.By the way that the sample-adding end of micro-fluidic chip is nested in the device, primary sample-adding just can quickly measure many indexes, and also achieve while detecting the content of the Multiple components interfered there are immunological cross, furthermore, the device is with its flexible assembling mode, the micro-fluidic chip that different indexs can be detected according to the different demands nesting of hospital, carries out the combination of different Testing index, provides convenience for user terminal.
Description
Technical field
The utility model belongs to immune device for fast detecting field, embedding more particularly, to a kind of combined micro-fluidic chip
Covering device.
Background technique
Microfluidic chip technology proposes have basic operation units such as conventional chemical and biology from the nineties in last century
Integrated function on one chip.It is flowed in microchannel using controlled fluid, to realize in biological and chemical field
Detection.Development at full speed is obtained in the fields such as medicine and life science with its apparent advantage in recent years, in particular immediately, now
Field external diagnosis reagent industry provides new bright prospects.
Micro-fluidic immunofluorescence technique is that one in conjunction with immunological response principle in conjunction with micro-fluidic chip is used in vitro
The new technique of fast diagnosis reagent.The technology is mainly characterized by: high sensitivity, reproducible, reaction speed is fast.
Currently used for medicine field of fast detection micro-fluidic, quick detection chip is immunized is mostly single sense channel.I
The single channel micro-fluidic chip that company uses is shown in the Chinese patent application No. is CN201110132696.X, and presently, there are disadvantages
Are as follows: the content of the plurality of target ingredient interfered there are immunological cross can not be detected, and single channel is inadequate for reagent exploitation combination
Flexibly, it will increase and grind again then due to the intrinsic demand of high precision inside micro-fluidic chip such as exploitation multichannel micro-fluidic chip
The difficulty and cost for sending out design, considerably increase the time cost and economic cost of enterprise's technology development.
Utility model content
In view of this, the utility model is directed to a kind of combined micro-fluidic chip nested arrangement, by by miniflow
The sample-adding end of control chip is nested in the device, and primary sample-adding just can quickly measure many indexes, not only can solve multiple fingers
The work for the very complicated being repeatedly loaded is marked, saves manpower and the cost of time, and with its flexible assembling mode, Ke Yigen
The micro-fluidic chip of different indexs is detected according to the different demands nesting of hospital, is neatly carried out the combination of different Testing index, is
User terminal provides convenience.
In order to achieve the above objectives, the technical solution of the utility model is achieved in that
A kind of combined micro-fluidic chip nested arrangement, including pedestal and the upper cover being fixedly arranged on pedestal, the side of pedestal
Wall is equipped at least two nested channels, and the top of nested channels communicates with the outside world, and the upper surface of upper cover is equipped with a sample-adding groove,
Upper cover offers the aggregation pond of several perforation upper covers, and aggregation pond is located at the outside of sample-adding groove and the sample-adding end of micro-fluidic chip and inserts
After entering nested channels, aggregation pond is located at the surface of the well of micro-fluidic chip, is loaded between groove and aggregation pond and is communicated with
Separating liquid channel.
Further, it is loaded between groove and separating liquid channel and is equipped with sample buffer, sample buffer includes buffering
Groove, the interior obstacles protrusion equipped with several for slowing down the flowing velocities of sample solution of buffer groove, obstacle protrusion is cylindrical,
Sample-adding groove, buffer groove, separating liquid channel are sequentially communicated.
Further, the cross section of pedestal is rectangle, nested channels altogether there are two, two nested channels are opened in side by side
Where the long side of pedestal on side wall, aggregation pond altogether there are two, two aggregation ponds are to being loaded being equidistant for groove.
Further, the cross section of pedestal is in equilateral triangle, and the number of nested channels is three, three nested channels difference
On three side walls of pedestal, assembles pond and separating liquid channel is circumferentially distributed in the outside of sample-adding groove.
Further, the cross section of pedestal is square, and the number of nested channels is four, and four nested channels distinguish position
In on four side walls of pedestal, aggregation pond and separating liquid channel are circumferentially distributed in the outside for being loaded groove.
Further, the cross section of pedestal is in regular pentagon, and the number of nested channels is five, five nested channels difference
On five side walls of pedestal, assembles pond and separating liquid channel is circumferentially distributed in the outside of sample-adding groove.
Further, the bottom parallel interval of nested channels is equipped with the elastic strip of two strip protrusions, the material of elastic strip
Matter is rubber.
Further, aggregation pond is in rounding mesa-shaped, and the upper cover of aggregation pond edge is downwardly extending lug boss, lug boss
Center be liquid channel, cross liquid channel and communicated with nested channels, the lowermost diameter of lug boss is less than micro-fluidic chip
The diameter of well.
Further, the volume for being loaded groove is 80-200 μ L, and the section semicircular in shape in separating liquid channel, aggregation pond is most
The diameter of upper end is 2mm, and the lowermost diameter is 1mm.
Further, the cross section of upper cover and the cross-sectional shape of pedestal are identical and equal in magnitude.
Compared with the existing technology, combined micro-fluidic chip nested arrangement described in the utility model has following excellent
Gesture:
(1) the utility model optimizes the micro-fluidic immunofluorescence chip of traditional single channel, by by micro-fluidic chip
Sample-adding end is nested in the device, primary sample-adding just can quickly measure many indexes, and development cost is low, is eliminated and is repeatedly loaded
Troublesome operation, and also achieve while detecting there are the content of the Multiple components of immunological cross interference, it is interfered for immunological cross
Or there are the Testing index of potential cross jamming to provide accurate and glitch-free detection mode, in addition, the device is flexible with it
Assembling mode can detect the micro-fluidic chip of different indexs according to the different demands nesting of hospital, carry out different Testing index
Combination, provide convenience for user terminal；
(2) the utility model can slow down the flowing velocity of sample solution, be conducive to sample by setting sample buffer
Solution is averagely diverted to separating liquid channel；
(3) the utility model is provided with elastic strip, and the sample-adding end of micro-fluidic chip can pass through bullet when being inserted into nested channels
Property item, elastic strip on the one hand can uniformly micro-fluidic chip insertion speed, on the other hand can make micro-fluidic chip with it is embedding
Agree with closer in set channel；
(4) the utility model by setting lug boss and crosses liquid channel, and sample is avoided to flow out into the lower surface of upper cover, and
And the lowermost diameter of lug boss is less than the diameter of the well of micro-fluidic chip, and sample is made accurately to be added drop-wise to micro-fluidic chip
It is loaded in the well at end.
Detailed description of the invention
The attached drawing for constituting a part of the utility model is used to provide a further understanding of the present invention, this is practical new
The illustrative embodiments and their description of type are not constituteed improper limits to the present invention for explaining the utility model.?
In attached drawing:
Fig. 1 is the overall structure diagram of combined micro-fluidic chip nested arrangement described in embodiment 1；
Fig. 2 is the enlarged drawing of part A in Fig. 1；
Fig. 3 is the cross-sectional view of combined micro-fluidic chip nested arrangement described in embodiment 1；
Fig. 4 is the top view of the pedestal of combined micro-fluidic chip nested arrangement described in embodiment 1；
Fig. 5 is that (elasticity is omitted in the top view of the pedestal of combined micro-fluidic chip nested arrangement as described in example 2
Item)；
Fig. 6 is the cross-sectional view of combined micro-fluidic chip nested arrangement as described in example 2；
Fig. 7 is the top view of the upper cover of combined micro-fluidic chip nested arrangement as described in example 2；
Fig. 8 is that (elasticity is omitted in the top view of the pedestal of combined micro-fluidic chip nested arrangement described in embodiment 3
Item)；
Fig. 9 is the top view of the upper cover of combined micro-fluidic chip nested arrangement described in embodiment 3；
Figure 10 is that (bullet is omitted in the top view of the pedestal of combined micro-fluidic chip nested arrangement as described in example 4
Property item)；
Figure 11 is the top view of the upper cover of combined micro-fluidic chip nested arrangement as described in example 4.
Description of symbols:
1, pedestal；11, nested channels；111, elastic strip；2, upper cover；21, it is loaded groove；22, sample buffer；221, delay
Rush groove；222, obstacle protrusion；23, separating liquid channel；24, assemble pond；241, lug boss.
Specific embodiment
It should be noted that in the absence of conflict, the feature in the embodiments of the present invention and embodiment can
To be combined with each other.
In the description of the present invention, it should be understood that term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of describing the present invention and simplifying the description, rather than indicate
Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand
For limitations of the present invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition
State the concrete meaning of term in the present invention.
The utility model will be described in detail below with reference to the accompanying drawings and embodiments.
Embodiment 1
A kind of combined micro-fluidic chip nested arrangement, as shown in Figure 1, including pedestal 1 and being fixedly arranged on upper on pedestal 1
Lid 2, the cross section of upper cover 2 are identical and equal in magnitude with the cross-sectional shape of pedestal 1.It is nested there are two being set on the side wall of pedestal 1
The top in channel 11, nested channels 11 communicates with the outside world, and the sample-adding end of micro-fluidic chip is inserted into nested channels 11.Two
Where a nested channels 11 are opened in the long side of pedestal 1 side by side on side wall.The upper surface of upper cover 2 is equipped with a sample-adding groove 21, adds
The volume of sample groove 21 is 80-200 μ L, preferably 100 μ L.The upper surface of upper cover 2 open up there are two perforation upper cover 2 aggregation pond
24, two aggregation ponds 24 are located at the outside of sample-adding groove 21 and two aggregation pond 24 being equidistant to sample-adding groove 21.
The upper surface of upper cover 2 is additionally provided with several connection sample-adding grooves 21 and assembles the separating liquid channel 23 in pond 24, liquid
The section semicircular in shape of split channel 23.Assemble pond 24 and nested channels 11 correspond, it, will after upper cover 2 is fixedly arranged on pedestal 1
In the sample-adding end insertion nested channels 11 of micro-fluidic chip, aggregation pond 24 is located at the surface of the well of micro-fluidic chip.To
The sample of detection is added sample-adding groove 21 and reaches aggregation pond 24 through the orientation drainage of split channel stream, then naturally falls in micro-fluidic
In the well at the sample-adding end of chip, to realize the shunting sample-adding of sample.
Referring to figs. 1 and 2, it is loaded between groove 21 and separating liquid channel 23 and is equipped with sample buffer 22, sample
Buffer area 22 includes buffer groove 221, is equipped with several columned obstacle protrusions 222, obstacle protrusion 222 in buffer groove 221
The flowing velocity that sample solution can be slowed down is conducive to sample solution and is averagely diverted to separating liquid channel 23, sample-adding groove 21,
Buffer groove 221, separating liquid channel 23 are sequentially communicated.
In conjunction with shown in Fig. 1 and Fig. 4, the bottom parallel interval of nested channels 11 is equipped with the elastic strip of two strip protrusions
111, the material of elastic strip 111 is rubber or other have the material of certain elasticity.When the sample-adding end of micro-fluidic chip is inserted into
In nested channels 11 can pass through elastic strip 111,111 one side of elastic strip can uniformly micro-fluidic chip insertion speed, it is another
It is closer that aspect can make micro-fluidic chip agree with nested channels 11.
As shown in figure 3, aggregation pond 24 is in rounding mesa-shaped, the diameter of aggregation 24 the top of pond is 2mm, the lowermost diameter is
1mm.The upper cover 2 of aggregation 24 edge of pond is downwardly extending lug boss 241, and the center of lug boss 241 was liquid channel, crosses liquid
Channel is communicated with nested channels 11, so set, sample is avoided to flow out into the lower surface of upper cover 2.The bottom of lug boss 241
Diameter be less than micro-fluidic chip well diameter, cannot accurately be added drop-wise to micro-fluidic chip sample-adding end to avoid sample
In well.
Working principle: before test sample, the present invention being horizontally arranged on workbench, and the sample-adding end of micro-fluidic chip is inserted into
In nested channels 11.When test sample, sample to be detected is added in sample-adding groove 21, sample is split through sample buffer 22
To separating liquid channel 23, aggregation pond 24 is reached through separating liquid channel 23, aggregated pond 24 and crosses liquid channel, is flowed into micro-fluidic
Chip is loaded in the well at end, is completed the shunting sample-adding process of sample, then can be completed correlation in micro-fluidic chip and refer to
Target detection.
Embodiment 2
In conjunction with shown in Fig. 5, Fig. 6 and Fig. 7, this embodiment differs from embodiment 1 in that, the cross section of pedestal 1 is in just
Triangle, the number of nested channels 11 are three, and three nested channels 11 are located on three side walls of pedestal 1, liquid point
The circumferential outside for being distributed in sample-adding groove 21 of circulation road 23.
Embodiment 3
In conjunction with shown in Fig. 8 and Fig. 9, this embodiment differs from embodiment 1 in that, the cross section of pedestal 1 is square,
The number of nested channels 11 is four, and four nested channels 11 are located on four side walls of pedestal 1, separating liquid channel 23
Circumferentially it is distributed in the outside of sample-adding groove 21.
Embodiment 4
In conjunction with shown in Figure 10 and Figure 11, this embodiment differs from embodiment 1 in that, the cross section of pedestal 1 is in positive five
Side shape, the number of nested channels 11 are five, and five nested channels 11 are located on five side walls of pedestal 1, separating liquid
The circumferential outside for being distributed in sample-adding groove 21 in channel 23.
Verify the homogeneity of combined micro-fluidic chip nested arrangement diverted liquid of the present invention:
After the combined micro-fluidic chip nested arrangement of 1~embodiment of embodiment 4 is inserted into micro-fluidic chip respectively, it is added
Enterprise's internal control product detect reaction signal value.In order to verify the combined micro-fluidic chip nesting dress of 1~embodiment of embodiment 4
The homogeneity of diverted liquid is set, is inserted into each nested channels respectively with the micro-fluidic chip of single index, detects same index respectively
Signal results.Using cTnI micro-fluidic chip as test object, 1ng/ml, the internal control of 10ng/ml, 50ng/ml cTnI enterprise are taken
Product, each point set 10 repetitions, count the mean concentration measured and the coefficient of variation (english abbreviation CV) value, as a result such as table 1
Shown in~table 4.
The testing result of 1 embodiment 1 of table
The testing result of 2 embodiment 2 of table
The measurement result of 3 embodiment 3 of table
The testing result of 4 embodiment 4 of table
By the above testing result it is found that the combined micro-fluidic chip nested arrangement of 1~embodiment of embodiment 4 with enterprise
The numerical value for controlling product in the industry is not much different, and illustrates there is good homogeneity using diverted liquid of the present invention.And the coefficient of variation is equal
Less than 10%, meet the precision requirement of detection.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model
Protection scope within.
Claims (10)
1. a kind of combined micro-fluidic chip nested arrangement, it is characterised in that: including pedestal (1) and be fixedly arranged on pedestal (1)
Upper cover (2), the side wall of pedestal (1) is equipped at least two nested channels (11), top and the external world's phase of nested channels (11)
Logical, the upper surface of upper cover (2) is equipped with sample-adding groove (21), and upper cover (2) offers the aggregation pond of several perforations upper cover (2)
(24), after aggregation pond (24) is located at the outside of sample-adding groove (21) and sample-adding end insertion nested channels (11) of micro-fluidic chip,
Aggregation pond (24) is located at the surface of the well of micro-fluidic chip, is loaded between groove (21) and aggregation pond (24) and is communicated with liquid
Body split channel (23).
2. combined micro-fluidic chip nested arrangement according to claim 1, it is characterised in that: sample-adding groove (21) and
Sample buffer (22) are equipped between separating liquid channel (23), sample buffer (22) include buffer groove (221), are buffered recessed
Obstacle in slot (221) equipped with several for slowing down the flowing velocity of sample solution is raised (222), and obstacle protrusion (222) is in circle
Column, sample-adding groove (21), buffer groove (221), separating liquid channel (23) are sequentially communicated.
3. combined micro-fluidic chip nested arrangement according to claim 2, it is characterised in that: pedestal (1) it is transversal
Face is rectangle, nested channels (11) altogether there are two, two nested channels (11) are opened in side where the long side of pedestal (1) side by side
On wall, there are two aggregations pond (24) altogether, two aggregation pond (24) being equidistant to sample-adding groove (21).
4. combined micro-fluidic chip nested arrangement according to claim 2, it is characterised in that: pedestal (1) it is transversal
Face is in equilateral triangle, and the number of nested channels (11) is three, and three nested channels (11) are located at three sides of pedestal (1)
On wall, aggregation pond (24) and separating liquid channel (23) is circumferentially distributed in the outside of sample-adding groove (21).
5. combined micro-fluidic chip nested arrangement according to claim 2, it is characterised in that: pedestal (1) it is transversal
Face is square, and the number of nested channels (11) is four, and four nested channels (11) are located at four side walls of pedestal (1)
On, aggregation pond (24) and separating liquid channel (23) is circumferentially distributed in the outside of sample-adding groove (21).
6. combined micro-fluidic chip nested arrangement according to claim 2, it is characterised in that: pedestal (1) it is transversal
Face is in regular pentagon, and the number of nested channels (11) is five, and five nested channels (11) are located at five sides of pedestal (1)
On wall, aggregation pond (24) and separating liquid channel (23) is circumferentially distributed in the outside of sample-adding groove (21).
7. according to combined micro-fluidic chip nested arrangement described in 3,4,5,6 any one of claim, it is characterised in that:
The bottom parallel interval of nested channels (11) is equipped with the elastic strip (111) of two strip protrusions, and the material of elastic strip (111) is
Rubber.
8. according to combined micro-fluidic chip nested arrangement described in 3,4,5,6 any one of claim, it is characterised in that:
Assembling pond (24) is in rounding mesa-shaped, and the upper cover (2) of aggregation pond (24) edge is downwardly extending lug boss (241), lug boss
(241) center was liquid channel, crosses liquid channel and communicates with nested channels (11), the lowermost diameter of lug boss (241) is small
In the diameter of the well of micro-fluidic chip.
9. combined micro-fluidic chip nested arrangement according to claim 8, it is characterised in that: sample-adding groove (21)
Volume is 80-200 μ L, and the section semicircular in shape of separating liquid channel (23), the diameter of aggregation pond (24) the top is 2mm, most
The diameter of lower end is 1mm.
10. according to combined micro-fluidic chip nested arrangement described in 3,4,5,6 any one of claim, feature exists
In: the cross section of upper cover (2) is identical and equal in magnitude with the cross-sectional shape of pedestal (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201822024226.1U CN209333776U (en) | 2018-12-04 | 2018-12-04 | A kind of combined micro-fluidic chip nested arrangement |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201822024226.1U CN209333776U (en) | 2018-12-04 | 2018-12-04 | A kind of combined micro-fluidic chip nested arrangement |
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| Publication Number | Publication Date |
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| CN209333776U true CN209333776U (en) | 2019-09-03 |
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ID=67750496
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112221545A (en) * | 2020-12-10 | 2021-01-15 | 天津德祥生物技术有限公司 | Multi-channel microfluidic sample adding device and application thereof |
-
2018
- 2018-12-04 CN CN201822024226.1U patent/CN209333776U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112221545A (en) * | 2020-12-10 | 2021-01-15 | 天津德祥生物技术有限公司 | Multi-channel microfluidic sample adding device and application thereof |
| CN112221545B (en) * | 2020-12-10 | 2021-03-16 | 天津德祥生物技术有限公司 | Multi-channel microfluidic sample adding device and application thereof |
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