CN209193969U - A kind of electronics classification inoculation apparatus of self-heating sterilizing - Google Patents
A kind of electronics classification inoculation apparatus of self-heating sterilizing Download PDFInfo
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- CN209193969U CN209193969U CN201821974157.4U CN201821974157U CN209193969U CN 209193969 U CN209193969 U CN 209193969U CN 201821974157 U CN201821974157 U CN 201821974157U CN 209193969 U CN209193969 U CN 209193969U
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Abstract
The utility model discloses a kind of electronics classification inoculation apparatus of self-heating sterilizing, it includes main body, the main body includes: handheld component, insulating layer, heating element and inoculation component, one end of the handheld component is connect with one end of the insulating layer, the other end of the insulating layer is connect with the heating element, the inoculation component is arranged in the end connection of the heating element, is provided with sterilizing control assembly in the handheld component, power supply component and charging assembly are provided in the handheld component.The utility model structure is simple, it is convenient to operate, it is reusable, Special sterilizing sterilizing installation is eliminated the reliance on, experimental implementation is greatly facilitated, risk is low, it is provided simultaneously with overcharge and over-discharge Electricity Functional, securely and reliably, and using the design of replaceable oese/needle, it is extensive suitable for multiple uses, the application prospect such as plate streaking and puncture bacterium preparation.
Description
Technical field
The utility model relates to a kind of electronics classification inoculation apparatus of self-heating sterilizing, in particular to a kind of reusable, no
The common experimental instrument electronics oese or transfer needle for relying on alcolhol burner (open fire) sterilization belong to Protocols in Molecular Biology neck
Domain.
Background technique
Common a kind of inoculating tool when oese (needle) is Bacteria Culture, it is micro- to be widely used in microorganism detection, cell
Numerous ambits such as biology, molecular biology.Using in escherichia expression system expression and purification Protein Assav, using connecing
It is extremely common step that kind ring (needle) does puncture to protect bacterium and scribing line recovery to bacterial strain.Existing oese (needle) presses material not
It is same to generally can be divided into plastics oese (needle) and metal oese (needle).Disposable plastics oese (needle) is easy to use, but
As medical disposable material higher operating costs, and using it is rear it is discarded before need to do harmless processing, seldom become preferred experiment equipment；
Metal oese (needle) is reusable, with preceding and must carry out after flame calcination sterilizing, usually uses alcolhol burner or coal gas
Pass through on the flame of lamp, sterilized rear can be used, and due to complicated for operation cumbersome, and contact open fire, exist in experimentation compared with
High security risk.And since the safety problem of alcolhol burner is often to denounce, and the bacterium during calcination on oese (needle)
It is easy to flash, easily pollution Experimental Area and sample.It is popular in recent years to use electric heating oese sterilizer to metal oese (needle)
Carry out disinfection sterilizing, although avoiding open fire operation, due to ceramics heating is slow and application method is cumbersome etc., still cannot
The selection best as experiment.
Utility model content
The main purpose of the utility model is to provide a kind of electronics classification inoculation apparatus of self-heating sterilizing, to overcome existing skill
Deficiency in art.
For realization foregoing purpose, the technical solution adopted in the utility model includes:
The utility model embodiment provides a kind of electronics classification inoculation apparatus of self-heating sterilizing, including main body, the main body
It include: handheld component, insulating layer, heating element and inoculation component, one end of one end of the handheld component and the insulating layer
Connection, the other end of the insulating layer are connect with the heating element, and the inoculation is arranged in the end connection of the heating element
Component is provided with sterilizing control assembly in the handheld component, power supply component and charging is provided in the handheld component
Component.
In some embodiments, one end of the handheld component is connect with one end of the heating element, also, described
Insulating layer is covered in the regional area of the heating element.
In some embodiments, the power supply component includes rechargeable lithium battery.
Further, the sterilizing control assembly includes sterilizing switch.
Further, the insulating layer includes ceramic insulating layer.
In some embodiments, the inoculation component is flexibly connected with the heating element, and the inoculation component includes
Transfer needle or oese.
In some embodiments, the electronics classification inoculation apparatus of self-heating sterilizing further include: rotary seal cap, it is described
The first matching screw thread is provided on insulating layer, the rotary seal cap is internally provided with the second matching screw thread, the rotatory sealing
It covers and matches the mutual cooperation realization sealed connection that screw thread matches screw thread with second by first with the insulating layer.
Further, the heating element includes heater strip, and the heater strip includes nickel filament.
Further, the electronics classification inoculation apparatus of self-heating sterilizing further include: intelligent UI control system is at least used
With anti-overcharge, control heating time or amplification operating current.
Further, the electronics classification inoculation apparatus of self-heating sterilizing further include: telescopic component, the telescopic component are set
It is placed between the handheld component and heating component.
Further, the electronics classification inoculation apparatus of self-heating sterilizing further include: guard assembly, the guard assembly set
Set on the body exterior, at least to protect the main body.
Further, the rotary seal cap medial end is additionally provided with limited block, and buffering is coated on the limited block
Layer, at least the inoculation component is limited and be buffered.
Compared with prior art, the utility model has the beneficial effects that:
1) the electronics classification inoculation apparatus structure of self-heating sterilizing provided by the utility model is simple, and it is convenient to operate, repeatable to make
With eliminating the reliance on Special sterilizing sterilizing installation, risk is low, securely and reliably, greatly facilitates experimental implementation；
2) rechargeable lithium battery built in the electronics classification inoculation apparatus of self-heating sterilizing provided by the utility model, it is light small and exquisite,
It is easy to use, it is charged using USB interface, versatility is good；
3) UI control system of the electronics classification inoculation apparatus of self-heating sterilizing provided by the utility model using intelligence, heating
15s is automatically powered off, more safe, also extends the service life of battery, and thermal transition is high-efficient, and low energy consumption, is provided simultaneously with
Charging and over-discharge Electricity Functional, securely and reliably；
4) the electronics classification inoculation apparatus of self-heating sterilizing provided by the utility model uses the design of replaceable oese/needle,
Suitable for plate streaking and the multiple uses such as bacterium preparation are punctured, it is widely used；
5) the electronics classification inoculation apparatus of self-heating provided by the utility model sterilizing configures sealing cover, effective protection heating part
Part, while preventing operator from accidentally touching heating region and causing to scald.
Detailed description of the invention
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The some embodiments recorded in application, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings.
Fig. 1 is that a kind of main structure of the electronics classification inoculation apparatus of self-heating sterilizing in one exemplary embodiments of the utility model is shown
It is intended to.
Fig. 2 be in one exemplary embodiments of the utility model a kind of insulating layer of the electronics classification inoculation apparatus of self-heating sterilizing and plus
The structural schematic diagram of thermal part.
Fig. 3 is a kind of rotary seal cap of the electronics classification inoculation apparatus of self-heating sterilizing in one exemplary embodiments of the utility model
Structural schematic diagram.
Description of symbols: 1- handheld component, 1a- sterilizing switch, 1b-USB charge port, 2- ceramic insulating layer, 2a- first
Match screw thread, 2b- extensible member, 3- heater strip, 4a- oese, 4b- transfer needle, 5- rotary seal cap, the matching spiral shell of 5a- second
Line.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Detailed description, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole implementation
Example.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work
The every other embodiment obtained, fall within the protection scope of the utility model.
Please refer to the electronics classification inoculation apparatus that Fig. 1-Fig. 3 show the sterilizing of one of the present embodiment self-heating comprising hand
Hold component 1 (i.e. hand grip), ceramic insulating layer 2, heater strip 3, replaceable inoculation component and rotary seal cap 5.The hand
The one end for holding component 1 is connect with one end of ceramic insulating layer 2, and the other end of ceramic insulating layer 2 is connect with the heater strip 3, institute
The end for stating heater strip 3 connects replaceable inoculation component, and the inoculation component includes transfer needle 4b or oese 4a.The hand
It holds and is provided with sterilizing control assembly on component 1, is i.e. sterilizing switch 1a, power supply component is provided in the handheld component and fills
Electrical component, specially USB charge port 1b, are charged using USB interface, and versatility is good.
It wherein, include a rechargeable lithium battery, and corresponding smart circuit inside the hand grip of main body with anti-
It overcharges, control the functions such as heating time, amplification operating current.Rechargeable lithium battery built in the utility model, it is light small and exquisite, it uses
It is convenient.
3 part of heater strip connecting with replaceable transfer needle 4b is made of nickel filament, and intensity highly corrosion resistant has both replaceable
Oese (needle) function, enough length can cope with the single tube of plurality of specifications.3 leading portion of heater strip is wrapped in ceramic insulating layer 2
It is interior, operator's accidentally touching scald is prevented while protecting heater strip.
In the present embodiment, the rotary seal cap 5 is made of heat resistant plastice, by configuring sealing cover, effective protection
Heater strip can further protect heating part, while preventing operator from accidentally touching heating region and causing to scald.
In the present embodiment, using the UI control system of intelligence, heating 15s is automatically powered off, more safe, is also extended
The service life of battery.Using smart circuit, thermal transition is high-efficient, and low energy consumption.It is provided simultaneously with overcharge and over-discharge Electricity Functional, is pacified
It is complete reliable.
In the present embodiment, using the design of replaceable oese/needle, it is more to be suitable for plate streaking and puncture bacterium preparation etc.
Kind purposes.
Further, it is provided with the first matching screw thread 2a on the ceramic insulating layer, is arranged inside the rotary seal cap 5
There is the second matching screw thread 5a, the rotary seal cap 5 matches screw thread 2a by first with the ceramic insulating layer 2 and matches with second
The mutual cooperation of screw thread 5a, which is realized, to be tightly connected.
Further, the also settable telescopic component of electronics classification inoculation apparatus of the self-heating sterilizing of the present embodiment, it is described flexible
Component is set between the handheld component and heater strip, at least to the length for adjusting heater strip, can according to the actual situation into
Row adjustment in time, applicability is wider, operates more flexible.
Further, the also settable guard assembly of electronics classification inoculation apparatus of the self-heating sterilizing of the present embodiment, the protection
Assembly set is set to the body exterior, at least to protect the main body.
Further, 5 medial end of rotary seal cap is additionally provided with limited block, and buffering is coated on the limited block
Layer, at least the inoculation component is limited and be buffered.
The electronics classification inoculation apparatus of the self-heating sterilizing of the present embodiment needs to charge before first use, can connect after primary charging
It is continuous to use up to tens of times.It impregnates after 75% ethyl alcohol flicking sterilizing switch several seconds, until oese (needle) position is heated to burn red, pine
Switch, ring (needle) to be seeded can pick bacterium solution after slightly cooling down at room temperature, cross or puncture to solid medium.Long-pressing
Sterilizing switch is more than 15s, and protection circuit can be automatically cut off the power, and prevents unexpected generation.
Using the electronics classification inoculation apparatus of the self-heating sterilizing in the present embodiment, with pet28a (+) escherichia expression system
For expression and purification MMLV-RT Protein Assav, the concrete operation step when carrying out scribing line screening and cloning and preparation punctures bacterium is such as
Under:
1. preparation: it is carried out disinfection using the sprinkling of 75% ethyl alcohol or ultraviolet light irradiation to Experimental Area, electronics oese
It is charged in advance；
2. taking out a glycerol stock frozen from -80 DEG C of refrigerators, slightly thaw at room temperature；
3. unscrewing oese sealing cover, 75% ethyl alcohol will be soaked in possibly into the part in cryopreservation tube when all inoculations
In；
Rubescent, the trip switch 4. long-pressing sterilizing switch to oese position is heated, it is slightly cooling at room temperature；
5. oese is protruded into cryopreservation tube, first picking a small amount of bacterium solution in nozzle is fully cooled oese, then is deeply stained with
Take bacterium solution；
6. a pair partial region is crossed, 4 are then repeated the above steps；
7. oese is in Kan+Plating medium edge is cooling once, then successively crosses to other regions；
8. repeating step 3 and 4 back spins closely covering, the plate for pulling line is placed in 37 DEG C of incubators and is incubated overnight；
9. next day makees the disinfection treatment of step 1 to test area, electronics transfer needle is charged in advance；
10. 75% ethanol disinfection is immersed at transfer needle position, then repeatedly step 4；
11. transfer needle is cooling once at plating medium edge, individually full stalwartness is cloned in Kan to picking+Single tube culture
In base, repeats step 3 and 4 back spins closely cover；
12. the single tube for connecting bacterium is placed in shaking table, 37 DEG C of 220rpm are cultivated to OD600=0.6；
13. unscrewing transfer needle sealing cover, will be soaked in 75% ethyl alcohol when all inoculations possibly into the part in single tube,
Repeat step 4；
14. transfer needle is protruded into single tube, first picking on a small quantity on bacterium solution top is fully cooled transfer needle, then deeply picks
Bacterium solution；
15. unscrewing a new Kan+Bacterium culture medium is punctured, is pierced into vertically 2~3 times with the puncture needle for being stained with bacterium, step is repeated
3 and 4 back spins closely cover；
16. the puncture culture medium for connecting bacterium is placed in 37 DEG C of incubator cultures to the visible obvious mycelia of naked eyes.
By above-mentioned technical proposal, the electronics classification inoculation apparatus structure of the self-heating sterilizing of the utility model is simple, and operation is just
Victory, it is reusable, Special sterilizing sterilizing installation is eliminated the reliance on, experimental implementation is greatly facilitated, risk is low, is provided simultaneously with
Charging and over-discharge Electricity Functional securely and reliably, and using the design of replaceable oese/needle are suitable for plate streaking and puncture
Multiple uses, the application prospects such as bacterium preparation are extensive.
The technology contents and technical characteristic of the utility model have revealed that as above, however those skilled in the art still may be used
Can the announcement based on the utility model and make various replacements and modification without departing substantially from the spirit of the present invention, therefore, this is practical new
Type protection scope should be not limited to the revealed content of embodiment, and including the various replacements without departing substantially from the utility model and should repair
Decorations, and covered by present patent application claim.
Claims (10)
1. a kind of electronics classification inoculation apparatus of self-heating sterilizing, including main body, which is characterized in that the main body include: handheld component,
Insulating layer, heating element and inoculation component, one end of the handheld component are connect with one end of the insulating layer, the insulating layer
The other end connect with the heating element, the inoculation component, the hand-held part is arranged in the connection of the end of the heating element
It is provided with sterilizing control assembly on part, power supply component and charging assembly are provided in the handheld component.
2. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1, it is characterised in that: the one of the handheld component
End is connect with one end of the heating element, also, the insulating layer is covered in the regional area of the heating element.
3. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1 or 2, it is characterised in that: the power supply
Component includes rechargeable lithium battery.
4. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1, it is characterised in that: the sterilizing control assembly
Including sterilizing switch；And/or the insulating layer includes ceramic insulating layer.
5. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1, it is characterised in that: the inoculation component and institute
Heating element flexible connection is stated, the inoculation component includes transfer needle or oese.
6. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1, it is characterised in that further include: rotatory sealing
It covers, is provided with the first matching screw thread on the insulating layer, the rotary seal cap is internally provided with the second matching screw thread, the rotation
Turn sealing cover and matches the mutual cooperation realization sealed connection that screw thread matches screw thread with second by first with the insulating layer.
7. the electronics classification inoculation apparatus of self-heating according to claim 1 sterilizing, it is characterised in that: the heating element includes
Heater strip, the heater strip include nickel filament.
8. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1, it is characterised in that further include: intelligent UI control
System, at least to anti-overcharge, control heating time or amplification operating current.
9. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 1, it is characterised in that further include: telescopic component,
The telescopic component is set between the handheld component and heating component.
10. the electronics classification inoculation apparatus of self-heating sterilizing according to claim 6, it is characterised in that further include: guard assembly,
The guard assembly is sheathed on the body exterior, at least to protect the main body；On the inside of the rotary seal cap
End is additionally provided with limited block, and buffer layer is coated on the limited block, at least to the inoculation component carry out limit and
Buffering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821974157.4U CN209193969U (en) | 2018-11-28 | 2018-11-28 | A kind of electronics classification inoculation apparatus of self-heating sterilizing |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821974157.4U CN209193969U (en) | 2018-11-28 | 2018-11-28 | A kind of electronics classification inoculation apparatus of self-heating sterilizing |
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| CN209193969U true CN209193969U (en) | 2019-08-02 |
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| CN201821974157.4U Active CN209193969U (en) | 2018-11-28 | 2018-11-28 | A kind of electronics classification inoculation apparatus of self-heating sterilizing |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234577A (en) * | 2021-04-14 | 2021-08-10 | 齐齐哈尔医学院 | Inoculating loop for microbial culture in microbial detection |
| CN113337379A (en) * | 2021-07-09 | 2021-09-03 | 青岛滨海学院 | Intelligent adjustable self-heating inoculator for microbial experiment |
-
2018
- 2018-11-28 CN CN201821974157.4U patent/CN209193969U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234577A (en) * | 2021-04-14 | 2021-08-10 | 齐齐哈尔医学院 | Inoculating loop for microbial culture in microbial detection |
| CN113337379A (en) * | 2021-07-09 | 2021-09-03 | 青岛滨海学院 | Intelligent adjustable self-heating inoculator for microbial experiment |
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