CN209010520U - Adjustable speed constant temperature tissue dissociation device - Google Patents
Adjustable speed constant temperature tissue dissociation device Download PDFInfo
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- CN209010520U CN209010520U CN201821409660.5U CN201821409660U CN209010520U CN 209010520 U CN209010520 U CN 209010520U CN 201821409660 U CN201821409660 U CN 201821409660U CN 209010520 U CN209010520 U CN 209010520U
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- tube body
- constant temperature
- adjustable speed
- speed constant
- pipe
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Abstract
The utility model discloses a kind of adjustable speed constant temperature tissue dissociation device, including dissociation pipe and reaction unit, pipe is dissociated by tube body and pipe Gai Zucheng, reaction unit includes crust of the device, the display panel being installed on crust of the device, the control module being connect with display panel and the reaction plate being placed in crust of the device, reaction plate is equipped with reacting hole, tube body inner circumferential is equipped at intervals with several raised structures, raised structures are located at the neighbouring one end of tube body far from pipe lid, the fixed structure that the bottom of reacting hole is provided with rotating device and is fixedly connected with rotating device, tube body is connected and fixed with fixed structure, control module is used to control the temperature of reaction plate, heating time, the revolving speed of rotating device, rotational time.The adjustable speed constant temperature tissue dissociation device of the utility model is applicable to the dissociation of the tissue of different type and volume, reduces the manual operation in experimentation, more effectively forms single cell suspension, improves conventional efficient.
Description
Technical field
The utility model relates to Bioexperiment instrument fields, and in particular to a kind of adjustable speed constant temperature tissue dissociation device.
Background technique
Originally culture is directly to be cultivated from a part that organism obtains tissue or organ, also referred to as Initial culture.?
The tissue dispersion for needing will acquire during this is at individual cells.There are mainly two types of the methods of dispersion, and one is with physics
Method directly pulverizes tissue, and the cell dispersion efficiency of this mode is lower, and may cause one to cell in operating process
Fixed mechanical damage, final available cell concentration are less；Another is to be handled using reagents such as enzymes tissue block, processing
It is allowed to be dispersed into individual cells by mechanical method again afterwards, this method is relatively mild, but time-consuming relatively long, it is also desirable to
A large amount of manual operation.
Utility model content
The purpose of this utility model is to provide a kind of adjustable speed constant temperature tissue dissociation devices, can be according to the need of experimenter
The cell by histological types and volume is asked to be dispersed into single cell suspension automatically, dissociation process high degree of automation, more rapidly,
Effect is more preferable.
For this purpose, the utility model uses following technical scheme:
There is provided a kind of adjustable speed constant temperature tissue dissociation device, including dissociation pipe and reaction unit, the dissociation pipe by tube body with
Pipe Gai Zucheng, the reaction unit include crust of the device, the display panel being installed on described device shell and the display surface
The control module of plate connection and the reaction plate being placed in described device shell, the reaction plate are equipped with reacting hole, feature
It is that the tube body inner circumferential is equipped at intervals with several raised structures, the raised structures are located at the neighbouring tube body far from the pipe lid
One end, the fixed structure that the bottom of the reacting hole is provided with rotating device and is fixedly connected with the rotating device is described
Tube body is connected and fixed with the fixed structure, and the control module is used to control the temperature of the reaction plate, heating time, described
The revolving speed and rotational time of rotating device.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, axis of the raised structures relative to the tube body
Line is centrosymmetric, and the quantity of the raised structures is no less than 8.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, axis of the raised structures close to the tube body
One side be zigzag.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, axis of the raised structures close to the tube body
One side and be serrated along the axial direction of the tube body.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, axis of the raised structures close to the tube body
One side and be serrated along the radial direction of the tube body.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, the material of the dissociation pipe tube body and the pipe lid
For polypropylene.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, the display panel is LED display.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, the fixed structure passes through with the rotating device
Screw is fixedly connected.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, the diameter of the reacting hole is than the fixed structure
Diameter it is big, the diameter of the tube body is smaller than the diameter of the reacting hole, and the tube body and the fixed structure are interference fitted.
As a kind of preferred embodiment of adjustable speed constant temperature tissue dissociation device, the fixed structure is TPE material.
The utility model has the beneficial effects that the adjustable speed constant temperature tissue dissociation device of the utility model is mainly made using water flow
Tissue block is driven all around to rotate around center in tube wall at being vortexed, scratching and rubbing by tissue block and tube wall protrusion, from
And gradually by tissue dispersion at individual cells, reaction temperature, rotation speed and reaction time can be carried out during reaction
Personalization is adjusted, to meet the dissociation demand of the tissue of different type, volume, the both hands of thorough liberation experimenter, and can
Single cell suspension is fast and effeciently formed, subsequent operation is convenient for.
Detailed description of the invention
It, below will be in the utility model embodiment in order to illustrate more clearly of the technical solution of the utility model embodiment
Required attached drawing is briefly described.It should be evident that drawings described below is only the one of the utility model
A little embodiments for those of ordinary skill in the art without creative efforts, can also be according to these
Attached drawing obtains other attached drawings.
Fig. 1 is the stereoscopic schematic diagram of adjustable speed constant temperature tissue dissociation device described in an embodiment of the present invention.
Fig. 2 is the structural schematic diagram that pipe is dissociated in Fig. 1.
Fig. 3 is the partial enlarged view of part A in Fig. 2.
Fig. 4 is the diagrammatic cross-section of fixed structure and rotating device in reacting hole in Fig. 1.
In figure:
1, dissociation pipe；11, tube body；12, Guan Gai；13, raised structures；2, reaction unit；21, crust of the device；22, display surface
Plate；23, control module；24, reaction plate；25, reacting hole；251, fixed structure；252, rotating device.
Specific embodiment
Further illustrate the technical solution of the utility model below with reference to the accompanying drawings and specific embodiments.
Wherein, the drawings are for illustrative purposes only and are merely schematic diagrams, rather than pictorial diagram, should not be understood as to this
The limitation of patent；In order to better illustrate the utility model embodiment, the certain components of attached drawing have omission, zoom in or out,
The size of actual product is not represented；It will be understood by those skilled in the art that certain known features and its explanation may save in attached drawing
Slightly it will be understood by.
The same or similar label correspond to the same or similar components in the attached drawing of the utility model embodiment；It is practical at this
In novel description, it is to be understood that if there is the orientation of the instructions such as term " on ", "lower", "left", "right", "inner", "outside"
Or positional relationship is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of description the utility model and simplification is retouched
It states, rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation,
Therefore the terms describing the positional relationship in the drawings are only for illustration, should not be understood as the limitation to this patent, for this
For the those of ordinary skill in field, the concrete meaning of above-mentioned term can be understood as the case may be.
In the description of the present invention, unless otherwise clearly defined and limited, if there is the instruction such as term " connection "
Connection relationship between component, which shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection,
Or it is integral；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be indirect by intermediary
It is connected, can be the connection of two components interiors or the interaction relationship of two components.For the ordinary skill people of this field
For member, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As shown in Figure 1, the embodiments of the present invention provide a kind of adjustable speed constant temperature tissue dissociation device, including dissociation pipe 1
And reaction unit 2, dissociation pipe 1 are made of tube body 11 and pipe lid 12, reaction unit 2 includes crust of the device 21, is installed on outside device
Display panel 22, the control module 23 being connect with display panel 22 on shell 21 and the reaction plate being placed in crust of the device 21
24, reaction plate 24 is equipped with reacting hole 25, and 11 inner circumferential of tube body is equipped at intervals with several raised structures 13, and raised structures 13 are located at neighbouring
The one end of tube body 11 far from pipe lid 12, the bottom of reacting hole 25 are provided with rotating device 252 and are fixedly connected with rotating device 252
Fixed structure 251, tube body 11 connects and fixes with fixed structure 251, control module 23 be used to control reaction plate 24 temperature,
Heating time, rotating device 252 revolving speed and rotational time, operator will be cut into tissue block, the enzyme of several fritters when operation
And reaction solution is put into dissociation pipe 1, tightens pipe lid 12, and dissociation pipe 1 is fixed in the fixed structure 251 of reacting hole 25,
Be arranged suitable reaction temperature, revolving speed and time start experiment reaction: tissue block structure under the action of enzyme become loosely, due to
Dissociating the spin of pipe 1 drives the liquid in pipe that will form vortex, and the intracorporal tissue block of liquid is as vortex rotation is by the prominent of tube wall surrounding
It plays structure 13 gradually to scrape, and is finally dispersed into individual cells, which avoids strong mechanism to thin
Born of the same parents cause to damage, while reducing the manual operation of experimenter.
Control module 23 in the present embodiment is PLC controller, the temperature control module including being connect with reaction plate 24 and with
The speed control module that rotating device 252 connects, is the prior art, is specifically no longer described in detail.
Raised structures 13 can be there are many form be arranged, in the present embodiment, as shown in Fig. 2, raised structures 13 are relative to pipe
The axis of body 11 is centrosymmetric, and the quantity of raised structures 13 is no less than 8, as the quantity that raised structures 13 are arranged increases,
The effect number that protrusion and tissue block can be increased, quickly peels off structural cell.
Specifically, raised structures 13 are set as base portion (raised structures 13 and 11 junction of tube body) width, intermediate to inner process
Class triangle, the cell that can avoid scraping is stuck in base portion.
As shown in figure 3, raised structures 13 are serrated close to the one side of the axis of tube body 11 in the present embodiment, can add
Strong raised structures 13 scrape effect to histiocytic, quickly cell dispersion.
Specifically, raised structures 13 close to the axis of tube body 11 one side and be serrated along the axial direction of tube body 11.
Further, raised structures 13 close to the axis of tube body 11 one side and be serrated along the radial direction of tube body 11
(not shown) can further strengthen raised structures 13 and scrape effect to histiocytic.
In the present embodiment, reaction plate 24 is made of aluminium alloy, and faster, crust of the device 21 is made of heatpiston, can for heat transfer
Heat loss is effectively reduced, guarantees that reaction maintains required temperature progress, is conducive to enzyme reaction effect.
In the present embodiment, tube body 11 is screwed on pipe lid 12 by screw thread, and pipe lid 12 and tube body 11 form enclosed construction, can
It effectively prevent sample spillage or outside contamination source in experimentation to enter.
Specifically, dissociation 1 appearance of pipe is no different with common experimental centrifuge tube, and the material of tube body 11 and pipe lid 12 is poly- third
Alkene, tissue or cell will not be adhered on tube body 11 or pipe lid 12, and the diameter for dissociating pipe 1 can be fabricated to according to different demands
A variety of regular sizes such as 15mL, 50mL are adapted to related experiments supporting equipment (such as centrifugal device), facilitate subsequent to slender
Experiment consumptive material is saved in the operation that born of the same parents' dispersion liquid is precipitated and is resuspended.
In the present embodiment, display panel 22 is LED display, when can show reaction temperature, heating time, revolving speed and rotation
Between.
Such as Fig. 4, in the present embodiment, fixed structure 251 is fixed by screws with rotating device 252, fixed structure 251
Raw spin motion can be issued in the drive of rotating device 252.
Specifically, the diameter of fixed structure 251 is big in the diameter than hole of reacting hole 25, and the diameter of tube body 11 is than reacting hole 25
Diameter it is small, tube body 11 and fixed structure 251 are interference fitted, and the fixed structure 251 connect with rotating device 252 is TPE material,
It is heat-resisting, and have certain elasticity, dissociation pipe 1 can be wrapped up after stretching, extension, to have certain fixed effect to dissociation pipe 1, work as rotation
Fixed device is driven to rotate together with when device 252 rotates together with dissociation pipe 1.
The present embodiment adjustable speed constant temperature tissue dissociation device has the advantage that
1, the structure of the present apparatus is simple, and manufacturing cost is low；
2, the dissociation pipe plasticity of the utility model is strong, may be produced that specification identical with existing consumptive material, is suitable for a variety of
Existing laboratory apparatus is convenient for subsequent operation, saves experiment consumables cost；
3, personalized adjusting can be carried out to reaction temperature, rotation speed and reaction time, meet different type, the group of volume
The dissociation demand knitted；
4, the both hands of thorough liberation experimenter reduce manual operation, and can fast and effeciently form single cell suspension.
It is to be understood that above-mentioned specific embodiment is only the preferred embodiment and institute's application technology of the utility model
Principle.It will be understood by those skilled in the art that various modifications, equivalent replacement, variation etc. can also be done to the utility model.But
It is that these transformation should be all within the protection scope of the utility model without departing from the spirit of the utility model.In addition, this
Some terms used in application specification and claims are not limitation, it is only for convenient for description.
Claims (10)
1. a kind of adjustable speed constant temperature tissue dissociation device, including dissociation pipe (1) and reaction unit (2), the dissociation pipe (1) is by tube body
(11) include crust of the device (21), be installed on described device shell (21) with pipe lid (12) composition, the reaction unit (2)
It display panel (22), the control module (23) being connect with the display panel (22) and is placed in described device shell (21)
Reaction plate (24), the reaction plate (24) are equipped with reacting hole (25), which is characterized in that tube body (11) inner circumferential is equipped at intervals with
Several raised structures (13), the raised structures (13) are located at the one end of neighbouring tube body (11) far from the pipe lid (12),
The fixed knot that the bottom of the reacting hole (25) is provided with rotating device (252) and is fixedly connected with the rotating device (252)
Structure (251), the tube body (11) connect and fix with the fixed structure (251), and the control module (23) is described for controlling
The temperature of reaction plate (24), heating time, the rotating device (252) revolving speed and rotational time.
2. adjustable speed constant temperature tissue dissociation device according to claim 1, which is characterized in that several raised structures (13)
Axis relative to the tube body (11) is centrosymmetric, and the quantity of the raised structures (13) is no less than 8.
3. adjustable speed constant temperature tissue dissociation device according to claim 1, which is characterized in that the raised structures (13) are close
The one side of the axis of the tube body (11) is zigzag.
4. adjustable speed constant temperature tissue dissociation device according to claim 3, which is characterized in that the raised structures (13) are close
It the one side of the axis of the tube body (11) and is serrated along the axial direction of the tube body (11).
5. adjustable speed constant temperature tissue dissociation device according to claim 3, which is characterized in that the raised structures (13) are close
It the one side of the axis of the tube body (11) and is serrated along the radial direction of the tube body (11).
6. adjustable speed constant temperature tissue dissociation device according to claim 1, which is characterized in that the tube body (11) and the pipe
The material for covering (12) is polypropylene.
7. adjustable speed constant temperature tissue dissociation device according to claim 1, which is characterized in that the display panel (22) is
LED display.
8. adjustable speed constant temperature tissue dissociation device according to claim 1, which is characterized in that the fixed structure (251) with
The rotating device (252) is fixed by screws.
9. adjustable speed constant temperature tissue dissociation device according to claim 1, which is characterized in that the diameter of the reacting hole (25)
Diameter than the fixed structure (251) is big, and the diameter of the tube body (11) is smaller than the diameter of the reacting hole (25), the pipe
Body (11) and the fixed structure (251) are interference fitted.
10. adjustable speed constant temperature tissue dissociation device according to claim 9, which is characterized in that the fixed structure (251) is
TPE material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821409660.5U CN209010520U (en) | 2018-08-29 | 2018-08-29 | Adjustable speed constant temperature tissue dissociation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821409660.5U CN209010520U (en) | 2018-08-29 | 2018-08-29 | Adjustable speed constant temperature tissue dissociation device |
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| Publication Number | Publication Date |
|---|---|
| CN209010520U true CN209010520U (en) | 2019-06-21 |
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ID=66831911
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821409660.5U Active CN209010520U (en) | 2018-08-29 | 2018-08-29 | Adjustable speed constant temperature tissue dissociation device |
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| CN (1) | CN209010520U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106982A (en) * | 2021-12-28 | 2022-03-01 | 成都诺医德医学检验实验室有限公司 | Tissue separator and method of use |
-
2018
- 2018-08-29 CN CN201821409660.5U patent/CN209010520U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106982A (en) * | 2021-12-28 | 2022-03-01 | 成都诺医德医学检验实验室有限公司 | Tissue separator and method of use |
| CN114106982B (en) * | 2021-12-28 | 2023-11-10 | 成都诺医德医学检验实验室有限公司 | Tissue separator and method of use thereof |
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