A kind of localized cell culture ware
Technical field
The utility model relates to Bioexperiment fixture fields, in particular to a kind of localized cell culture ware.
Background technique
Currently, using culture dish often in various medical experiments to cultivate microorganism or cell.Usual situation
Under, after cell inoculation is into culture dish, due to the flowing of culture solution non-directional or the plane inequality various reasons of culture dish holding
It is difficult to ensure that the cell density of culture dish different location is completely the same, such phenomenon is outstanding before adherent for adherent growth cell
It is obvious.It is that the several visuals field of random observation are come greatly every time when experimenter needs dynamic observation cell or microbial morphology
Body determines cellular morphology or cell density, does not ensure that is observed every time is all the target of the same area, in this way since also hardly possible
To accomplish that a certain determining regional aim carries out accurately dynamic observation.
In another example needing to mark multiple scratches in culture dish in scratch experiment, to verify proliferation, the migration feelings of cell
Condition.Using conventional cell culture dish, when cell carries out dynamic observation to scratch, a visual field can only observe a scratch
Part, be difficult positioning when specific location of the downward view inner cell in this scratch, when microscope magnification increases, this
Kind phenomenon is more obvious.So, just it is difficult to ensure that is observed every time is all the cell of same scratch same position, it is different
Observation result may lack comparativity and continuity because of the position of observation difference.
Utility model content
The purpose of this utility model is to provide a kind of localized cell culture wares, are implementing cell correlation using the localized cell culture ware
During experiment, staff can target area in localized cell culture ware, consequently facilitating experimenter is accurately to a certain solid
Determining region cell or microorganism is stimulated, and is also convenient for carrying out dynamic sight to the form of this fixed area cell or microorganism
It examines.Thereby, it is possible to convenient for the accurate recording to Experimental Area, avoid causing to test number because stimulating every time or viewing area is different
According to error is generated, so as to obtain more accurate experimental result.
The embodiments of the present invention are achieved in that
A kind of localized cell culture ware, including upper cover, ontology and the positioning disk set gradually;Upper cover removably connects with ontology
It connects, ontology is detachably connected with positioning disk;
Ontology is made of clear material, and configured with culture cavity and opening, upper cover is used for opening cooperation to close ontology
Cultivate cavity;
Positioning disk includes rectangular frame, the first adjustment bar, second adjustment bar and positioning sleeve;Positioning sleeve is right against ontology
One end is provided with positioning surface;
The first adjustment bar and second adjustment bar are with the mobilizable connection of rectangular frame, and the first adjustment bar is along the first default side
To setting, and the first adjustment bar is used for along the second preset direction relative to frame movement;Second adjustment frame is along the second default side
To setting, and second adjustment bar is used for along the first preset direction relative to frame movement;First preset direction and second is preset
Direction is vertical;
Positioning sleeve is flexibly connected with the first adjustment bar and second adjustment bar, so that the first adjustment bar can drive positioning sleeve phase
Second adjustment bar is moved along the second preset direction, second adjustment bar can drive positioning sleeve relative to the first adjustment bar along first
Preset direction movement.
The localized cell culture ware includes the upper cover set gradually, ontology and positioning disk.Wherein, it is provided in ontology for training
Support the culture cavity of microorganism or cell.In addition, upper cover is detachably connected with ontology, ontology removably connects with positioning disk
It connects, is cooperated by the opening at upper cover and culture cavity, culture cavity can be closed;And pass through positioning disk and ontology
Connection can position, in order to specific in the process of test to testing or observing position in culture cavity
Position is observed, and is especially recorded to the position of experiment, in order to obtain accurate experimental data.
Specifically, for convenient for the positioning to experimental site, positioning disk includes rectangular frame, the first adjustment bar, second adjustment
Bar and positioning sleeve;One end that positioning sleeve is right against ontology is provided with positioning surface, since ontology is made of clear material, so energy
Enough the position of the positioning surface positioned at body bottom portion is observed through the bottom of ontology, from there through the position of positioning surface, so as to
Enough determine specific experimental site.Outside it, in order to enable the position of positioning surface, can be located at the experimental site in culture cavity
Lower section, therefore need to be adjusted the position of positioning surface, the first adjustment bar and second adjustment bar can live with rectangular frame as a result,
Dynamic connection, the first adjustment bar are arranged along the first preset direction, and the first adjustment bar be used for along the second preset direction relative to
Frame movement;Second adjustment frame is arranged along the second preset direction, and second adjustment bar be used for along the first preset direction relative to
Frame movement;First preset direction is vertical with the second preset direction.Positioning sleeve and the first adjustment bar and second adjustment bar activity connect
It connects, so that the first adjustment bar can drive positioning sleeve to move relative to second adjustment bar along the second preset direction, second adjustment bar
Positioning sleeve can be driven to move relative to the first adjustment bar along the first preset direction.Pass through the first adjustment bar and second adjustment as a result,
Bar is respectively adjusted position of the positioning surface on the first preset direction and the second preset direction, can be to the position of positioning surface
The corrective action realized planar is set, above-mentioned positioning action thus can be played.
In an embodiment of the present invention:
It is provided with and is respectively used to for the first adjustment bar and the movable first sliding groove of second adjustment bar and second sliding slot on frame,
First sliding groove is arranged along the second preset direction, and second sliding slot is arranged along the first preset direction.
In an embodiment of the present invention:
First sliding groove and second sliding slot are rectangular channel;
The body of rod of the first adjustment bar and second adjustment bar is respectively the rectangle with first sliding groove and second sliding slot corresponding matching
The body of rod.
In an embodiment of the present invention:
The be arranged respectively along the second preset direction and the first preset direction on the side wall of first sliding groove and second sliding slot
One guide groove and the second guide groove;
The first guide pad for cooperating with the first guide groove is provided on the first adjustment bar;
The second guide pad for cooperating with the second guide groove is provided on second adjustment bar.
In an embodiment of the present invention:
The both ends of the first adjustment bar and second adjustment bar include the hand-held part stretched out outside frame.
In an embodiment of the present invention:
Outer peripheral surface in multiple hand-held parts is provided with external screw thread, and the lock sleeve with external screw thread cooperation;
Lock sleeve is used for and frame is against to limit the activity of the first adjustment bar and second adjustment bar relative to frame.
In an embodiment of the present invention:
The side that hand-held part is right against ontology is provided with reference line, is provided with graduation mark on frame, to pass through reference line phase
The operating range of the first adjustment bar and second adjustment bar is measured for the position of graduation mark.
In an embodiment of the present invention:
When ontology is connect with positioning disk, positioning surface is bonded with the bottom of ontology.
In an embodiment of the present invention:
Rectangle or round wire are labeled on positioning surface around the profile of positioning surface.
In an embodiment of the present invention:
Positioning disk is made of clear material.
The technical solution of the utility model at least has the following beneficial effects:
Localized cell culture ware provided by the utility model can be during implementing cell related experiment, in localized cell culture ware
Target area be also convenient for pair consequently facilitating experimenter accurately stimulates a certain fixed area cell or microorganism
The form of this fixed area cell or microorganism carries out dynamic observation.Thereby, it is possible to be convenient for the accurate recording to Experimental Area,
It avoids causing experimental data to generate error because stimulating every time or viewing area is different, so as to obtain more accurate experiment
As a result.
Detailed description of the invention
It, below will be to use required in embodiment in order to illustrate more clearly of the technical solution of the utility model embodiment
Attached drawing be briefly described, it should be understood that the following drawings illustrates only some embodiments of the utility model, therefore should not be by
Regard the restriction to range as, for those of ordinary skill in the art, without creative efforts, may be used also
To obtain other relevant attached drawings according to these attached drawings.
Fig. 1 is the structural schematic diagram of localized cell culture ware in the utility model embodiment;
Fig. 2 is the structural schematic diagram of ontology in the utility model embodiment;
Fig. 3 is the structural schematic diagram at the first visual angle of rectangular frame in the utility model embodiment;
Fig. 4 is the structural schematic diagram at the first visual angle of rectangular frame in the utility model embodiment;
Fig. 5 is the structural schematic diagram at the second visual angle of rectangular frame in the utility model embodiment;
Fig. 6 is the partial enlargement diagram in Fig. 5 at VI.
Icon: 200- localized cell culture ware;210- upper cover;220- ontology;230- positioning disk;221- cultivates cavity;222- is opened
Mouthful;231- rectangular frame;232- the first adjustment bar;233- second adjustment bar;234- positioning sleeve;235- positioning surface;236- first
Sliding slot;The 237- rectangle body of rod;The hand-held part 238-;239- external screw thread;241- lock sleeve;242- reference line;243- graduation mark;244-
Rectangle wire.
Specific embodiment
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is the utility model a part of the embodiment, instead of all the embodiments.Usually here in attached drawing description and
The component of the utility model embodiment shown can be arranged and be designed with a variety of different configurations.
Therefore, requirement is not intended to limit to the detailed description of the embodiments of the present invention provided in the accompanying drawings below
The scope of the utility model of protection, but it is merely representative of the selected embodiment of the utility model.Based in the utility model
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the range of the utility model protection.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the utility model embodiment, it should be noted that the orientation of the instructions such as term "inner", "lower" or
Positional relationship be based on the orientation or positional relationship shown in the drawings or the utility model product using when the side usually put
Position or positional relationship, are merely for convenience of describing the present invention and simplifying the description, rather than the device of indication or suggestion meaning
Or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to the utility model
Limitation.In addition, term " first ", " second " etc. are only used for distinguishing description, it is not understood to indicate or imply relative importance.
In the description of the utility model embodiment, it is also necessary to which explanation is unless specifically defined or limited otherwise,
Term " setting ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection;It can be mechanical connection, can be directly connected, two elements can also be can be indirectly connected through an intermediary
Internal connection.For the ordinary skill in the art, above-mentioned term can be understood in the utility model with concrete condition
In concrete meaning.
Embodiment
Currently, using culture dish often in various medical experiments to cultivate microorganism or cell.Usual situation
Under, after cell inoculation is into culture dish, due to the flowing of culture solution non-directional or the plane inequality various reasons of culture dish holding
It is difficult to ensure that the cell density of culture dish different location is completely the same, such phenomenon is outstanding before adherent for adherent growth cell
It is obvious.It is that the several visuals field of random observation are come greatly every time when experimenter needs dynamic observation cell or microbial morphology
Body determines cellular morphology or cell density, does not ensure that is observed every time is all the target of the same area, in this way since also hardly possible
To accomplish that a certain determining regional aim carries out accurately dynamic observation.
In another example needing to mark multiple scratches in culture dish in scratch experiment, to verify proliferation, the migration feelings of cell
Condition.Using conventional cell culture dish, when cell carries out dynamic observation to scratch, a visual field can only observe a scratch
Part, be difficult positioning when specific location of the downward view inner cell in this scratch, when microscope magnification increases, this
Kind phenomenon is more obvious.So, just it is difficult to ensure that is observed every time is all the cell of same scratch same position, it is different
Observation result may lack comparativity and continuity because of the position of observation difference.
For these reasons, the purpose of this utility model is to provide a kind of localized cell culture ware 200, the localized cell culture is utilized
Ware 200 during implementing cell related experiment, staff can target area in localized cell culture ware 200, consequently facilitating
Experimenter accurately stimulates a certain fixed area cell or microorganism, is also convenient for this fixed area cell or micro-
The form of biology carries out dynamic observation.Thereby, it is possible to convenient for the accurate recording to Experimental Area, avoid because of stimulation or observation every time
Region is different and experimental data is caused to generate error, so as to obtain more accurate experimental result.
Fig. 1-6 is please referred to, Fig. 1-6 shows the specific structure of the localized cell culture ware 200 provided in embodiment.
Include the upper cover 210 set gradually, ontology 220 and determine from can be seen that the localized cell culture ware 200 in Fig. 1-6
Position disk 230.
Wherein, upper cover 210 and ontology 220 are detachably connected, and ontology 220 and positioning disk 230 are detachably connected.
Specifically, please referring to Fig. 1-2, ontology 220 is made of clear material, and ontology 220 is configured with culture cavity 221 and opens
Mouth 222, upper cover 210 are used to cooperate with opening 222 to close culture cavity 221.
Referring to figure 2. -5, positioning disk 230 include rectangular frame 231, the first adjustment bar 232, second adjustment bar 233 and
Positioning sleeve 234;One end that positioning sleeve 234 is right against ontology 220 is provided with positioning surface 235.
The first adjustment bar 232 and second adjustment bar 233 with the mobilizable connection of rectangular frame 231, the first adjustment bar 232
It is arranged along the first preset direction (in Fig. 5 shown in arrow direction), and the first adjustment bar 232 is used for along second preset direction (Fig. 5
Shown in middle arrow direction) relative to frame movement;Second adjustment frame is arranged along the second preset direction, and second adjustment bar 233
For along the first preset direction relative to frame movement;First preset direction is vertical with the second preset direction.
Positioning sleeve 234 is flexibly connected with the first adjustment bar 232 and second adjustment bar 233, so that the first adjustment bar 232 can
Positioning sleeve 234 is driven to move relative to second adjustment bar 233 along the second preset direction, second adjustment bar 233 can drive positioning sleeve
234 move relative to the first adjustment bar 232 along the first preset direction.
The localized cell culture ware 200 includes upper cover 210, ontology 220 and the positioning disk 230 set gradually.Wherein, ontology
The culture cavity 221 for cultivating microorganism or cell is provided in 220.In addition, upper cover 210 and ontology 220 are removably
Connection, ontology 220 and positioning disk 230 are detachably connected, are cooperated by the opening 222 at upper cover 210 and culture cavity 221,
Culture cavity 221 can be closed;And pass through the connection of positioning disk 230 and ontology 220, it can work as in the process of test
In, it is positioned to testing or observing position in culture cavity 221, in order to observe specific position, especially
The position of experiment is recorded, in order to obtain accurate experimental data.
Specifically, for convenient for the positioning to experimental site, positioning disk 230 include rectangular frame 231, the first adjustment bar 232,
Second adjustment bar 233 and positioning sleeve 234;One end that positioning sleeve 234 is right against ontology 220 is provided with positioning surface 235, due to this
Body 220 is made of clear material, so the positioning surface 235 positioned at 220 bottom of ontology can be observed through the bottom of ontology 220
Position, from there through the position of positioning surface 235, so as to the specific experimental site of determination.Outside it, in order to enable positioning surface
235 position, can be located at the lower section of the experimental site in culture cavity 221, therefore the position to positioning surface 235 is needed to adjust
It is whole, as a result, the first adjustment bar 232 and second adjustment bar 233 with the mobilizable connection of rectangular frame 231, the first adjustment bar 232
It is arranged along the first preset direction, and the first adjustment bar 232 is used for along the second preset direction relative to frame movement;Second adjustment
Frame is arranged along the second preset direction, and second adjustment bar 233 is used for along the first preset direction relative to frame movement;First is pre-
Set direction is vertical with the second preset direction.Positioning sleeve 234 is flexibly connected with the first adjustment bar 232 and second adjustment bar 233, so that
Obtaining the first adjustment bar 232 can drive positioning sleeve 234 to move relative to second adjustment bar 233 along the second preset direction, second adjustment
Bar 233 can drive positioning sleeve 234 to move relative to the first adjustment bar 232 along the first preset direction.Pass through the first adjustment bar as a result,
232 and second adjustment bar 233 position of the positioning surface 235 on the first preset direction and the second preset direction is adjusted respectively
It is whole, corrective action planar can be realized to the position of positioning surface 235, thus can play above-mentioned positioning action.
It in the present embodiment, referring to figure 2. -5, is the movement convenient for the first adjustment bar 232 and second adjustment bar 233, therefore
It is provided with and is respectively used to for the first adjustment bar 232 and the movable first sliding groove 236 of second adjustment bar 233 and second sliding slot on frame
(not shown), first sliding groove 236 are arranged along the second preset direction, and second sliding slot is arranged along the first preset direction.Also, it is
Prevent the first adjustment bar 232 and second adjustment bar 233 from rotating in first sliding groove 236 and second sliding slot respectively, therefore first
Sliding slot 236 and second sliding slot are rectangular channel;The body of rod of the first adjustment bar 232 and second adjustment bar 233 is respectively to slide with first
The rectangle body of rod 237 of slot 236 and second sliding slot corresponding matching.
In addition, since the first adjustment bar 232 and second adjustment bar 233 are long rod type structure, therefore in order to keep the first tune
The both ends of whole bar 232 and second adjustment bar 233 are moved synchronously along the second preset direction and the first preset direction respectively, therefore first
It is respectively arranged with guide frame between sliding slot 236 and the first adjustment bar 232 and between second sliding slot and second adjustment bar 233,
To guide the movement of the first adjustment bar 232 and second adjustment bar 233, therefore divide on the side wall of first sliding groove 236 and second sliding slot
The first guide groove and the second guide groove not being arranged along the second preset direction and the first preset direction;On the first adjustment bar 232
It is provided with the first guide pad for cooperating with the first guide groove;It is provided on second adjustment bar 233 for being oriented to second
Second guide pad of slot cooperation.
In addition, please refer to Fig. 6, for convenient for operation, with to the first adjustment bar 232 and second adjustment bar 233 on frame
Position is adjusted, to play the role of adjusting the position of positioning surface 235, therefore the first adjustment bar 232 and second adjustment bar 233
Both ends include the hand-held part 238 stretched out outside frame.
Further, it is being adjusted by position of the hand-held part 238 to the first adjustment bar 232 and second adjustment bar 233
Meanwhile for convenient for the position of positioning surface 235 determine after, avoid the position of positioning surface 235 from changing, thus need to first adjust
Whole bar 232 and second adjustment bar 233 are limited relative to the movement of frame, therefore the outer peripheral surface in multiple hand-held parts 238 is all provided with
It is equipped with external screw thread 239, and the lock sleeve 241 cooperated with external screw thread 239;Lock sleeve 241 is used for and frame is against to limit
Activity of one the adjustment bar 232 and second adjustment bar 233 relative to frame.
Further, referring to figure 2. -5, it should be noted that during use, for convenient for the first adjustment bar
232 and the position of second adjustment bar 233 accurately recorded, it can it is marked by corresponding positional distance, therefore
The side that hand-held part 238 is right against ontology 220 is provided with reference line 242, graduation mark 243 is provided on frame, to pass through base
Directrix 242 measures the operating range of the first adjustment bar 232 and second adjustment bar 233 relative to the position of graduation mark 243.In addition,
It should be noted that in the present embodiment, the first preset direction and the second preset direction are equal to X-direction and Y direction.
Further, in the present embodiment, referring to figure 5., in order to facilitate observation of, therefore when ontology 220 is connect with positioning disk 230
When, positioning surface 235 is bonded with the bottom of ontology 220.Secondly, for the orientation range convenient for determining positioning surface 235, therefore around positioning surface
235 profile is labeled with rectangle or round wire on positioning surface 235.
In addition, to avoid the other structures of positioning disk 230 from influencing observation, therefore the structure of positioning disk 230 can be by transparent material
Material is made.
The above descriptions are merely preferred embodiments of the present invention, is not intended to limit the utility model, for this
For the technical staff in field, various modifications and changes may be made to the present invention.It is all in the spirit and principles of the utility model
Within, any modification, equivalent replacement, improvement and so on should be included within the scope of protection of this utility model.