CN208829650U - Primer case - Google Patents

Abstract

The utility model relates to a kind of primer cases, and for storing centrifuge tube and PCR pipe, primer case includes box body and supporting element.Box body is in hollow structure, has accommodating chamber, the side of box body offers the opening being connected to accommodating chamber.Supporting element is accommodated and is fixed in accommodating chamber, the surface of supporting element offers multiple through-hole units, through-hole unit includes first through hole and the second through-hole corresponding with first through hole, the diameter of first through hole is greater than the diameter of the second through-hole, centrifuge tube can be arranged in first through hole, and PCR pipe can be arranged in the second through-hole.Primer case provided by the utility model has preferable preservation effect.

Description

Primer case

Technical field

The utility model relates to field of biotechnology more particularly to a kind of primer cases.

Background technique

In PCR (gene magnification) experiment, primer stoste and primer working solution need to be usually made.Primer divides positive primer and bears Primer.When amplification, the hybrid working liquid of positive primer and negative primer need to be added in DNA solution, positive primer is used for fixed purpose piece The starting point of section, negative primer are used for the terminal of fixed purpose segment, so can be fixed to positive and negative primer DNA intermediate segment into Row amplification.

And general company synthesis positive primer and negative primer be it is powdered, therefore, before experiment, primer solution need to be carried out Preparation.Firstly, the primer stoste that solubility is 100UM is respectively prepared in positive primer and the dissolution of negative primer powder.Further take out part Positive primer stoste and negative primer stoste are diluted to the positive primer working solution and negative primer working solution of 20UM respectively.Finally, taking-up portion Divide positive primer working solution to be mixed with negative primer working solution, is fabricated to the hybrid working liquid of positive and negative primer, then carry out PCR amplification Experiment.Remaining positive primer stoste and negative primer stoste are usually stored in centrifuge tube, remaining positive primer working solution and negative are drawn Object working solution is usually stored in PCR pipe, in case the needs of subsequent experimental.However, in the prior art, PCR pipe and centrifuge tube Preserving type it is complicated, preservation effect is poor, leads to the storage of stoste and working solution and inconvenient to use.

Utility model content

Based on this, it is necessary to for traditional PCR pipe and the problem of centrifuge tube preserving type complexity, provide a kind of preservation effect The good primer case of fruit.

A kind of primer case, for storing centrifuge tube and PCR pipe, the primer case includes:

Box body in hollow structure has accommodating chamber, and the side of the box body is offered opens with what the accommodating chamber was connected to Mouthful；

Supporting element is accommodated and is fixed in the accommodating chamber, and the surface of the supporting element offers multiple through-hole units, institute Stating through-hole unit includes first through hole and the second through-hole corresponding with the first through hole, and the diameter of the first through hole is greater than institute The diameter of the second through-hole is stated, the centrifuge tube can be arranged in the first through hole, and it is logical that the PCR pipe can be arranged in described second Hole.

The supporting element is elastic rubber in one of the embodiments,.

The surface to the bottom of the accommodating chamber of the first through hole is arranged in the supporting element in one of the embodiments, Distance be equal to the box opening end bottom of the end face to the accommodating chamber distance.

The edge contour of the supporting element and the inner surface contour of the accommodating chamber match in one of the embodiments, So that the supporting element is fastened in the accommodating chamber.

The box body and the supporting element are rectangle in one of the embodiments, multiple through-hole unit edges The length direction interval of the box body is arranged to form sets of vias, the sets of vias be it is multiple, multiple sets of vias are along the box The width direction interval of body is arranged.

The protrusion of surface of the supporting element forms the spine of arc, the both ends of the spine in one of the embodiments, It is connect respectively with the edge of the first through hole and second through-hole in the same through-hole unit.

The edge protrusion of the first through hole forms first annular step in one of the embodiments, and described second is logical The edge protrusion in hole forms the second ring-shaped step.

It in one of the embodiments, further include end cap, the end cap is installed on the edge of the opening, to open or cover Cover the opening.

The side of the end cap is connect with the box body by articulate in one of the embodiments,.

The end cap side opposite with the hinge is provided with adsorption piece, the box body in one of the embodiments, The position opposite with the adsorption piece is provided with matching piece, and the adsorption piece is operationally adsorbed or separated with the matching piece.

Above-mentioned primer case, through-hole unit include first through hole and the second through-hole.In general, the diameter of centrifuge tube is greater than The diameter of PCR pipe.Therefore, by the way that the diameter of first through hole to be set greater than to the diameter of the second through-hole, and centrifuge tube is worn In in first through hole, PCR pipe is arranged in the second through-hole, and centrifuge tube and PCR pipe are accommodated in same primer case simultaneously It is interior.Further, the second through-hole is corresponding with first through hole, therefore same stoste and the working solution being equipped with by same stoste can be accommodated in In same through-hole unit, to prevent, stoste is not corresponding with working solution and consumes a longer time the problem of finding corresponding primer generation. Therefore, above-mentioned primer case can also can clearly be distinguished while realizing the centralized management and stacking of centrifuge tube and PCR pipe Stoste and working solution, and be allowed to correspond, thus there is preferable preservation effect.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of primer case in the utility model preferred embodiment；

Fig. 2 is the structural schematic diagram of the supporting element in primer case shown in FIG. 1.

Specific embodiment

The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model, It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms It realizes, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes to the utility model The understanding of disclosure is more thorough and comprehensive.

It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ", " right side " and similar statement are for illustrative purposes only.

Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term " and or " used herein includes Any and all combinations of one or more related listed items.

Referring to Fig. 1, the primer case 100 in the utility model preferred embodiment includes box body 110 and supporting element 120.Primer case 100 is for storing centrifuge tube 101 and PCR pipe 103.In PCR sequencing experiment, centrifuge tube 101 is 1.5ml EP (epoxy resin) centrifuge tube 101.The EP that PCR pipe 103 is 200ul is managed.The diameter of centrifuge tube 101 is greater than the straight of PCR pipe 103 Diameter.

Box body 110 is in hollow structure, has accommodating chamber 112.Box body 110 is for accommodating centrifuge tube 101 and PCR pipe 103.Tool Body, box body 110 can be made of metal, paper, glass or other high molecular materials.Specifically in the present embodiment, box body 110 be transparent EP pipe box.The pipe box made of EP material has preferable chemical-resistance, heat-resisting and electrical apparatus insulation performance, The service life of box body 110 can effectively be extended.In addition, set transparent EP pipe box for box body 110, operator can be with That more intuitively observes centrifuge tube 101 box body 110 inside and PCR pipe 103 from outside holds situation, so that operating Simply, convenient.The side of box body 110 offers the opening 114 being connected to accommodating chamber 112.Operator can will be centrifuged from opening 114 Pipe 101 and PCR pipe 103 are put into accommodating chamber 112.

Referring to Figure 2 together, supporting element 120 is accommodated and is fixed in accommodating chamber 112.Specifically, supporting element 120 can pass through Fastener is fixed in accommodating chamber 112, fixed knot can also partially be arranged accordingly in the inner wall of supporting element 120 and accommodating chamber 112 Structure, to realize the fixation of supporting element 120.Specifically in the present embodiment, the edge contour of supporting element 120 and accommodating chamber 112 is interior Wall profile matches, so that supporting element 120 is fastened in accommodating chamber 112.

By matching supporting element 120 with the inner wall of accommodating chamber 112, supporting element 120 is fastened in accommodating chamber 112, it can It avoids causing the cost of primer case 100 to increase because using extra fastener.

The surface of supporting element 120 offers multiple through-hole units 123.Through-hole unit 123 includes first through hole 121 and with the Corresponding second through-hole 122 of one through-hole 121.The diameter of first through hole 121 is greater than the diameter of the second through-hole 122.Centrifuge tube 101 can It is arranged in first through hole 121, PCR pipe 103 can be arranged in the second through-hole 122.Specifically, first through hole 121 and the second through-hole 122 Space set can also be closely arranged with edge.It can be spaced setting between multiple through-hole units 123, it can also be with side Edge is closely arranged.Specifically in the present embodiment, first through hole 121 and the second through-hole 122 in same through-hole unit 123 Interval is arranged, also interval setting between multiple through-hole units 123.Therefore, centrifuge tube 101 and PCR pipe 103 are respectively placed in When one through-hole 121 and the second through-hole 122, can prevent first through hole 121 and the second through-hole 122 because setting too closely due to cause Centrifuge tube 101 and PCR pipe 103 pick and place inconvenience.

When with stoste configuration work liquid, due to the presence of error, while the concentration between the more bottles of stostes configured is to deposit In difference, the concentration between more bottles of working solutions configured with different stoste is also to have differences.Therefore, in order to reduce reality Error is tested, so that test is more accurate, when by stoste configuration work liquid, optimal configuration method is in the same centrifuge tube 101 Stoste only configure the working solution in the same PCR pipe 103 always.Therefore in setting, first in same through-hole unit 123 is logical It hole 121 need to be corresponding with the second through-hole 122.Specifically, corresponded manner can be to be a variety of, for example, the in same through-hole unit 123 One through-hole 121 and the second through-hole 122 can be connected to by a relatively narrow groove and be corresponded to, can also will be in same through-hole unit 123 First through hole 121 and the spacing of the second through-hole 122 be set as relatively narrow, the spacing between different through-hole units 123 is set as It is wider.Or the edge shape of first through hole 121 and the second through-hole 122 in same through-hole unit 123 can also be arranged To be consistent, 121 shape of first through hole between different through-hole units 123 is different.Specifically in the present embodiment, supporting element 120 Protrusion of surface formed arc spine 125.The both ends of spine 125 respectively with the first through hole 121 in same through-hole unit 123 And second through-hole 122 edge connection.

Therefore the first through hole 121 and the second through-hole 122 in same through-hole unit 123 are corresponding by spine 125.Different is logical First through hole 121 and the second through-hole 122 in hole unit 123 is corresponding by different spines 125.Therefore, by the way that spine is arranged 125, the identification between same stoste and same working solution is improved, can prevent other stostes from obscuring with this working solution and leading Cause biggish experimental error.

In addition, the shape and diameter of first through hole 121 also need to match with centrifuge tube 101, the shape of the second through-hole 122 and Diameter matches with PCR pipe 103.To avoid liquid from flowing out out of centrifuge tube 101 and PCR pipe 103, in general, in centrifuge tube 101 and Centrifuge tube lid 102 and PCR pipe lid 104 are also respectively provided at the nozzle of PCR pipe 103.The diameter of centrifuge tube lid 102 is greater than centrifugation The diameter of the nozzle of pipe 101, and by the way of pressing, it is covered in the nozzle of centrifuge tube 101.Likewise, PCR pipe lid 104 Diameter is greater than the nozzle diameter of PCR pipe 103, and the mode of pressing also can be used, and is covered in the nozzle of PCR pipe 103.

In the present embodiment, the diameter of first through hole 121 is set smaller than to the diameter of centrifuge tube lid 102, second is led to The diameter in hole 122 is set smaller than the diameter of PCR pipe lid 104.Therefore, when centrifuge tube 101 is sealed and is arranged in first through hole When in 121, centrifuge tube lid 102 can be supported with the edge of first through hole 121, logical to prevent centrifuge tube 101 to be contained in first completely Cause pick-and-place difficulty big in hole 121.Similarly, also the diameter of the second through-hole 122 can be set smaller than the straight of PCR pipe lid 104 Diameter, it is difficult to prevent PCR pipe from picking and placing.

Therefore, by the way that the diameter of first through hole 121 to be set greater than to the diameter of the second through-hole 122, and by centrifuge tube 101 It is arranged in first through hole 121, PCR pipe 103 is arranged in the second through-hole 122, and centrifuge tube 101 and PCR pipe 103 are received simultaneously It is contained in same primer case 100.Further, the second through-hole 122 is corresponding with first through hole 121, thus same stoste and by The working solution that same stoste is equipped with can be accommodated in same through-hole unit 123, and to prevent, stoste is not corresponding with working solution and expends The problem of long period searching corresponding primer, occurs.Therefore, above-mentioned primer case 100 can realize centrifuge tube 101 and PCR pipe While 103 centralized management and stacking, stoste and working solution can be also clearly distinguished, and be allowed to correspond, thus had Preferable preservation effect.

In the present embodiment, supporting element 120 is elastic rubber.

Therefore, the diameter of settable first through hole 121 is slightly less than the diameter of centrifuge tube 101, and the diameter of the second through-hole 122 omits Less than the diameter of PCR pipe 103.When placing centrifuge tube 101 into primer case 100, bullet occurs for the inner wall of first through hole 121 Property deformation, expanded in diameter may make centrifuge tube 101 to be arranged in first through hole 121.Simultaneously as deformation effect, first through hole 121 inner wall will also generate extruding to centrifuge tube 101 to the elastic-restoring force of centrifuge tube 101, consolidate so that centrifuge tube 101 is stable Due in first through hole 121.Therefore can prevent under the influence of external environment, centrifuge tube 101 falls out of first through hole 121 and is led It causes the stoste in centrifuge tube 101 to pour out, pollutes working environment.Similarly, supporting element 120 is that elastic rubber can also be by PCR pipe 103 Stable is fixed in the second through-hole 122, causes working solution to fall to prevent PCR pipe 103 from falling out of primer case 100 Out, working environment is polluted.

In the present embodiment, the surface of first through hole 121 is arranged to the distance etc. of the bottom of accommodating chamber 112 in supporting element 120 In box body 110 be open 114 ends end face to the bottom of accommodating chamber 112 distance.

If being reference with ground, i.e., the height of supporting element 120 is equal with the height of box body 110.Therefore, supporting element 120 is received When being dissolved in accommodating chamber 112, the end face at 114 end of opening at the edge and box body 110 of first through hole 121 and the second through-hole 122 is In same level.It is placed in first through hole 121 when by centrifuge tube 101, PCR pipe 103 is placed in the second through-hole 122 When interior, the inner wall of accommodating chamber 112, which places centrifuge tube 101 and PCR pipe 103 to operator, can not generate barrier effect, consequently facilitating Operator's quick fetching centrifuge tube 101 and PCR pipe 103.

Further, in the present embodiment, the edge protrusion of first through hole 121 forms first annular step 126, and second is logical The edge protrusion in hole 122 forms the second ring-shaped step 127.

Specifically, the internal diameter of first annular step 126 be less than centrifuge tube lid 102 diameter, the second ring-shaped step 127 it is interior Diameter is less than the diameter of PCR pipe lid 104.When centrifuge tube 101 is contained in first through hole 121, centrifuge tube lid 102 is logical towards first The surface in hole 121 will be supported with the end of first annular step 126.First annular step 126 has support to centrifuge tube lid 102 And fixed function, may make centrifuge tube lid 102 be detached from supporting element 120 surface, and with the surface interval of supporting element 120 be arranged with Form gap.It, can be from gap to centrifuge tube 101 if centrifuge tube 101 is bonded closer with the end of first annular step 126 Apply an active force backwards to supporting element 120, centrifuge tube 101 is taken out.Therefore, by the way that first annular step 126 is arranged, It can prevent what centrifuge tube lid 102 was bonded with the surface of supporting element 120 too closely centrifuge tube 101 to be caused to pick and place difficulty.Similarly, The setting of second ring-shaped step 127 also may make the pick-and-place of PCR pipe 103 simple and easy.

In the present embodiment, box body 110 and supporting element 120 are rectangle, and multiple through-hole units 123 are along box body 110 Length direction interval is arranged to form sets of vias 124, sets of vias 124 be it is multiple, multiple sets of vias 124 are along the width side of box body 110 It is arranged to interval.

Rectangle is the conventional shape of box body 110 and supporting element 120.By setting long for box body 110 and supporting element 120 It is rectangular, it may make box body 110 and supporting element 120 to make simply, convenient for reducing production cost.It should be noted that in other realities It applies in example, box body 110 and supporting element 120 may be round, polygon or other irregular shapes.

Multiple sets of vias 124 are arranged along the width direction interval of box body 110, and the receiving of supporting element 120 can be made full use of empty Between, setting first through hole 121 as much as possible and the second through-hole 122, to store centrifuge tube 101 and PCR pipe 103, so as to reality The existing higher utilization efficiency of primer case 100.

In the present embodiment, primer case 100 further includes end cap 130.End cap 130 is installed on the edge of opening 114, with Open or cover opening 114.

Accommodating chamber 112 can be formed a closed storage environment by the end cap 130 for being covered in opening 114, to prevent the external world Steam, impurity etc. enter in accommodating chamber 112 storage for influencing the stoste in centrifuge tube 101 and the working solution in PCR pipe 103. When opening end cap 130, supporting element 120, centrifuge tube 101 and PCR pipe 103 can be dismantled or be installed from opening 114 by operator.

To achieve the effect that good fixed centrifuge tube 101 and PCR pipe 103, the height of end cap 130 can be with centrifuge tube lid The thickness of 102 thickness matching, the thickness and PCR pipe lid 104 of centrifuge tube lid 102 is consistent.Therefore, when end cap 130 covers When at opening 114, end cap 130 will be supported towards the surface of box body 110 with the surface of centrifuge tube lid 102 and PCR pipe lid 104, To be fixed on centrifuge tube 101 and PCR pipe 103 are stable in accommodating chamber 112.

Further, in the present embodiment, the side of end cap 130 is flexibly connected with box body 110 by hinge 140.

Hinge 140 drives the side of end cap 130 to rotate opening and closing, it can be achieved that opening 114.In addition, end cap 130 is logical Hinge 140 is crossed to be flexibly connected with box body 110, it can also be when opening opening 114 without dismantling end cap 130, so as to accelerate to draw The service speed of object case 100.

It should be noted that in other embodiments, the side of end cap 130 can also pass through shaft, connecting rod or other turns Dynamic structure is flexibly connected with the realization of box body 110.

Further, in the present embodiment, the side opposite with hinge 140 of end cap 130 is provided with adsorption piece 150.Box body 110 positions opposite with adsorption piece 150 are provided with matching piece 160, and adsorption piece 150 operationally adsorbs or divides with matching piece 160 From.

Adsorption piece 150 and matching piece 160 adsorb, and end cap 130 can be stably fixed to the edge of opening 114, to prevent from drawing When object case 100 vibrates, end cap 130 is opened, and centrifuge tube 101 and PCR pipe 103 are fallen out of box body 110.When adsorption piece 150 It separates, end cap 130 can be opened with matching piece 160.Specifically, adsorption piece 150 is not limited to be set to end cap 130 and hinge 140 Opposite side, in other embodiments, adsorption piece 150 may also be disposed on end cap 130 and remove any of setting 140 side of hinge Side, any two sides or even three sides can be with.The quantity of matching piece 160 and position need to be corresponding with adsorption piece 150.

Specifically, adsorption piece 150 and matching piece 160 can be magnet, velcro or other adsorption pieces 150 and matching piece 160 forms need to only ensure that adsorption piece 150 can be adsorbed and be separated with matching piece 160.

Above-mentioned primer case 100, through-hole unit 123 include first through hole 121 and the second through-hole 122.In general, centrifuge tube 101 diameter is greater than the diameter of PCR pipe 103.Therefore, by the way that the diameter of first through hole 121 is set greater than the second through-hole 122 Diameter, and centrifuge tube 101 is arranged in first through hole 121, PCR pipe 103 is arranged in the second through-hole 122, will be centrifuged Pipe 101 and PCR pipe 103 are accommodated in same primer case 100 simultaneously.Further, the second through-hole 122 and first through hole 121 is corresponding, therefore same stoste and the working solution being equipped with by same stoste can be accommodated in same through-hole unit 123, to prevent original Liquid is not corresponding with working solution and consumes a longer time the problem of finding corresponding primer generation.Therefore, above-mentioned primer case 100 can While realizing the centralized management and stacking of centrifuge tube 101 and PCR pipe 103, stoste and working solution can be also clearly distinguished, and It is allowed to correspond, thus there is preferable preservation effect.

Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited In contradiction, all should be considered as described in this specification.

Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed, But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.

Claims (10)

1. a kind of primer case, for storing centrifuge tube and PCR pipe, which is characterized in that the primer case includes:

Box body in hollow structure has accommodating chamber, and the side of the box body offers the opening being connected to the accommodating chamber；

Supporting element is accommodated and is fixed in the accommodating chamber, and the surface of the supporting element offers multiple through-hole units, described logical Hole unit includes first through hole and the second through-hole corresponding with the first through hole, and the diameter of the first through hole is greater than described the The diameter of two through-holes, the centrifuge tube can be arranged in the first through hole, and the PCR pipe can be arranged in second through-hole.

2. primer case according to claim 1, which is characterized in that the supporting element is elastic rubber.

3. primer case according to claim 1, which is characterized in that the table of the first through hole is arranged in the supporting element Face is equal to the distance of bottom of the end face at the box opening end to the accommodating chamber to the distance of the bottom of the accommodating chamber.

4. primer case according to claim 1, which is characterized in that the edge contour of the supporting element and the receiving The inner surface contour of chamber matches, so that the supporting element is fastened in the accommodating chamber.

5. primer case according to claim 4, which is characterized in that the box body and the supporting element are rectangular Shape, multiple through-hole units are arranged to form sets of vias along the length direction interval of the box body, the sets of vias be it is multiple, it is more A sets of vias is arranged along the width direction interval of the box body.

6. primer case according to claim 5, which is characterized in that the protrusion of surface of the supporting element forms arc Spine, the both ends of the spine edge with the first through hole and second through-hole in the same through-hole unit respectively Connection.

7. primer case according to claim 1, which is characterized in that the edge protrusion of the first through hole forms first The edge protrusion of ring-shaped step, second through-hole forms the second ring-shaped step.

8. primer case according to claim 1, which is characterized in that further include end cap, the end cap is installed on described The edge of opening, to open or cover the opening.

9. primer case according to claim 8, which is characterized in that the side of the end cap and the box body pass through hinge Chain is flexibly connected.

10. primer case according to claim 9, which is characterized in that the end cap side opposite with the hinge Be provided with adsorption piece, the box body position opposite with the adsorption piece is provided with matching piece, the adsorption piece operationally with The matching piece absorption or separation.