CN208815010U - A kind of device continuously preparing amino acid strain - Google Patents

A kind of device continuously preparing amino acid strain Download PDF

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Publication number
CN208815010U
CN208815010U CN201821044312.2U CN201821044312U CN208815010U CN 208815010 U CN208815010 U CN 208815010U CN 201821044312 U CN201821044312 U CN 201821044312U CN 208815010 U CN208815010 U CN 208815010U
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China
Prior art keywords
seed culture
culture tank
screw
amino acid
fastened together
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CN201821044312.2U
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Chinese (zh)
Inventor
高世军
吴泽华
王志强
李刚
罗明刚
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LINQING DENENG GOLDENCORN BIOLOGICAL Co Ltd
Shouguang Golden Corn Biotechnology Co Ltd
Shouguang Golden Far East Starch Co Ltd
SHANDONG SHOUGUANG JUNENG GROUP GOLDEN CORN CO Ltd
Original Assignee
LINQING DENENG GOLDENCORN BIOLOGICAL Co Ltd
Shouguang Golden Corn Biotechnology Co Ltd
Shouguang Golden Far East Starch Co Ltd
SHANDONG SHOUGUANG JUNENG GROUP GOLDEN CORN CO Ltd
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Priority to CN201821044312.2U priority Critical patent/CN208815010U/en
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Abstract

The utility model discloses a kind of devices for continuously preparing amino acid strain, including support base, seed culture tank, seeding tank, the seed culture tank is provided with above the support base, pressure inductor is provided with above the seed culture tank, the pressure inductor side is provided with filter, the filter side is provided with feed inlet, the seed culture tank is internally provided with temperature control plate, oxygen-supplying tube is provided on the temperature control plate, temperature inductor is provided with above the oxygen-supplying tube, exhaust valve is provided with above the temperature inductor, the seed culture tank side is provided with quick coupling, the quick coupling side is provided with inoculation mouth, pressure pump is provided with above the quick coupling.Beneficial effect is: solve microbiological contamination risk and the calcination loss to strain existing for shaking flask flame inoculation, this device ensure that the stability of strain, and it is simple to operate, inoculation efficiency is substantially increased, toxigenic capacity is reduced, it is practical.

Description

A kind of device continuously preparing amino acid strain
Technical field
The utility model relates to Spawn incubation equipment technical fields, continuously prepare amino acid strain more particularly to a kind of Device.
Background technique
Strain refers to the propagation material of edible mushroom mycelium and its growth substrate composition.Strain be divided into parent species (level-one kind), Original seed (second level kind) and cultivar (three-level kind) three-level.The useful strain of industrial fermentation, screening step include strain separating, just Sieve and secondary screening.The useful strain with certain ability is selected, prepared by also referred to as seed, refer to strain under certain condition, by expanding Big culture becomes the preparation process of the pure production strain with certain amount and quality.To make further expansion in access fermentor Biomass and synthetic product are used.The method that people take sterile working, certain edible mushroom from the microorganism mixed, individually Ground separates, this process is called strain separating.The mycelium obtained in the separation process is exactly pure culture after purification.? Under laboratory condition, method manually to make pure culture raised growth and breeding is called culture.
Amino acid fermentation culture at present is generally carried out by the way of batch inoculation, i.e., batch of seeds shaking flask is inoculated with one kind Sub- tank, vaccination ways are generally accessed using several shaking flasks under the protection of flames, main problem existing for such Spawn incubation equipment It is that vaccination ways are complicated, and operation difficulty is big, and there are microbiological contamination risks.It is greatly improved the operating cost of culture, therefore is needed A kind of novel device is invented to solve the above problems.
Utility model content
The purpose of this utility model is that solve the above-mentioned problems and provides one kind and continuously prepare amino acid strain Device.
The utility model achieves the above object through the following technical schemes:
A kind of device continuously preparing amino acid strain, including support base, seed culture tank, seeding tank, the support base Top is provided with the seed culture tank, is provided with pressure inductor, the pressure inductor one above the seed culture tank Side is provided with filter, and the filter side is provided with feed inlet, and the seed culture tank is internally provided with temperature control plate, described It is provided with oxygen-supplying tube on temperature control plate, temperature inductor is provided with above the oxygen-supplying tube, is provided with above the temperature inductor Exhaust valve, the seed culture tank side are provided with quick coupling, and the quick coupling side is provided with inoculation mouth, described quick Pressure pump is provided with above connector, the fast interface side is provided with stainless steel connecting tube, the stainless steel connecting tube top It is provided with steam sterilizer, the steam sterilizer side is provided with the seeding tank, is arranged on the seed culture tank wall There is observation window, is provided with master station below the observation window.
In above structure, when carrying out the operation of equipment strain, the support base can be first passed through by equipment and be mounted on specified training Position is supported, sets relative program by the master station later, operator can be put into related culture by the feed inlet Raw material, while the temperature in the seed culture tank is carried out by the temperature control plate and is adjusted, the oxygen-supplying tube can control in it Oxygen state, when carrying out the inoculation operation of strain, the pressure pump work carries out the tune of the seed culture tank internal pressure Section is imported strain in the seeding tank by the stainless steel connecting tube by the variation of pressure, to guarantee inoculation environment Stablize, the steam sterilizer can control the quick coupling and the stainless steel connecting tube, that is, complete entire inoculation Process.
In order to further increase the culture effect for the device for continuously preparing amino acid strain, the support base and the seed Culture tank is fastened together by screw, and the pressure inductor and the seed culture tank are fastenedly connected by screw Together, the pressure inductor operating temperature range is -10 DEG C~80 DEG C.
In order to further increase the culture effect for the device for continuously preparing amino acid strain, the filter and the seed Culture tank is fastened together by screw, and the feed inlet takes shape in the seed culture tank top, the filter mistake Filter accuracy rating is 20-75um.
In order to further increase the culture effect for the device for continuously preparing amino acid strain, the temperature control plate and the seed Culture tank is fastened together by screw, and the oxygen-supplying tube is fastened together with the temperature control plate by screw, institute Stating temperature control plate adjustable range is -5 DEG C~50 DEG C.
In order to further increase continuously prepare amino acid strain device culture effect, the temperature inductor with it is described Temperature control plate is fastened together by screw, and the exhaust valve is linked together with the temperature control plate by screw anchor, The quantity of the exhaust valve is 3.
In order to further increase continuously prepare amino acid strain device culture effect, the quick coupling with described kind Sub- culture tank is fastened together by screw, and the pressure pump is linked together with the seed culture tank by pipe collar.
In order to further increase the culture effect for the device for continuously preparing amino acid strain, the stainless steel connecting tube and institute It states quick coupling to be fastened together by screw, the steam sterilizer and the stainless steel connecting tube are fastened by screw It links together.
In order to further increase continuously prepare amino acid strain device culture effect, the seeding tank and it is described quickly Connector is fastened together by screw, and the observation window is embedded on the seed culture tank wall, the master station with The seed culture tank is fastened together by screw.
The utility model has the beneficial effects that: solving microbiological contamination risk existing for shaking flask flame inoculation and burning to strain Scaling loss is lost, this device ensure that the stability of strain, simple to operate, substantially increases inoculation efficiency, reduces and be trained This, it is practical.
Detailed description of the invention
Fig. 1 is a kind of main view for the device for continuously preparing amino acid strain of the utility model;
Fig. 2 is a kind of left view for the device for continuously preparing amino acid strain of the utility model;
Fig. 3 is a kind of top view for the device for continuously preparing amino acid strain of the utility model.
The reference numerals are as follows:
1, seeding tank;2, steam sterilizer;3, quick coupling;4, pressure pump;5, observation window;6, feed inlet;7, filter; 8, pressure inductor;9, exhaust valve;10, temperature inductor;11, oxygen-supplying tube;12, master station;13, temperature control plate;14, seed culture Tank;15, support base;16, inoculation mouth;17, stainless steel connecting tube.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing:
As shown in Figure 1-Figure 3, a kind of continuously to prepare the device of amino acid strain, including support base 15, seed culture tank 14, Seeding tank 1 is provided with seed culture tank 14 above support base 15, pressure inductor 8, pressure is provided with above seed culture tank 14 8 side of inductor is provided with filter 7, and 7 side of filter is provided with feed inlet 6, and seed culture tank 14 is internally provided with temperature control Plate 13, temperature control plate 13 are controlled for temperature, and oxygen-supplying tube 11 is provided on temperature control plate 13, and oxygen-supplying tube 11 contains for controlling oxygen in tank Amount, is provided with temperature inductor 10 above oxygen-supplying tube 11, and temperature inductor 10 is for detecting temperature in tank, on temperature inductor 10 Side is provided with exhaust valve 9, and 14 side of seed culture tank is provided with quick coupling 3, and 3 side of quick coupling is provided with inoculation mouth 16, Pressure pump 4 is provided with above quick coupling 3, for pressure pump 4 for changing pressure inside the tank, fast interface side is provided with stainless steel company Adapter tube 17, steam sterilizer 2 is provided with above stainless steel connecting tube 17, and steam sterilizer 2 is used for wet sterilization, steam sterilizer 2 sides are provided with seeding tank 1, and observation window 5 is provided on 14 outer wall of seed culture tank, master station 12 is provided with below observation window 5, Master station 12 is controlled for equipment.
In above structure, when carrying out the operation of equipment strain, support base 15 can be first passed through by equipment and be mounted on specified culture Position sets relative program by master station 12 later, and operator can be put into related culture raw material by feed inlet 6, together When the temperature in seed culture tank 14 carried out by temperature control plate 13 adjust, oxygen-supplying tube 11 can control the oxygen state in it, when into When the inoculation operation of row strain, the work of pressure pump 4 carries out the adjusting of 14 internal pressure of seed culture tank, will by the variation of pressure Strain is imported in seeding tank 1 by stainless steel connecting tube 17, and for the stabilization for guaranteeing inoculation environment, steam sterilizer 2 can be to quick Connector 3 and stainless steel connecting tube 17 are controlled, that is, complete entire seeded process.
In order to further increase the culture effect for the device for continuously preparing amino acid strain, support base 15 and seed culture tank 14 are fastened together by screw, and pressure inductor 8 is fastened together with seed culture tank 14 by screw, pressure 8 operating temperature range of inductor is -10 DEG C~80 DEG C, and filter 7 is fastened together with seed culture tank 14 by screw, Feed inlet 6 takes shape in 14 top of seed culture tank, and 7 filtering accuracy range of filter is 20-75um, and temperature control plate 13 and seed are trained It supports tank 14 to be fastened together by screw, oxygen-supplying tube 11 is fastened together with temperature control plate 13 by screw, temperature control plate 13 adjustable ranges be -5 DEG C~50 DEG C, temperature inductor 10 is fastened together with temperature control plate 13 by screw, exhaust valve 9 and Temperature control plate 13 is linked together by screw anchor, and the quantity of exhaust valve 9 is 3, and quick coupling 3 and seed culture tank 14 are logical Screw is crossed to be fastened together, pressure pump 4 is linked together with seed culture tank 14 by pipe collar, stainless steel connecting tube 17 with Quick coupling 3 is fastened together by screw, and steam sterilizer 2 and stainless steel connecting tube 17 are fastenedly connected by screw Together, seeding tank 1 is fastened together with quick coupling 3 by screw, and observation window 5 is embedded in 14 outer wall of seed culture tank On, master station 12 is fastened together with seed culture tank 14 by screw.
The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change And improvement, these various changes and improvements fall within the scope of the claimed invention.

Claims (8)

1. a kind of device for continuously preparing amino acid strain, it is characterised in that: including support base (15), seed culture tank (14), Seeding tank (1), support base (15) top are provided with the seed culture tank (14), set above the seed culture tank (14) It is equipped with pressure inductor (8), pressure inductor (8) side is provided with filter (7), filter (7) the side setting Have feed inlet (6), the seed culture tank (14) is internally provided with temperature control plate (13), is provided with logical oxygen on the temperature control plate (13) It manages (11), is provided with temperature inductor (10) above the oxygen-supplying tube (11), the row of being provided with above the temperature inductor (10) Air valve (9), seed culture tank (14) side are provided with quick coupling (3), and quick coupling (3) side is provided with inoculation Mouth (16), quick coupling (3) top are provided with pressure pump (4), and the quick coupling side is provided with stainless steel connecting tube (17), it is provided with steam sterilizer (2) above the stainless steel connecting tube (17), steam sterilizer (2) side is provided with The seeding tank (1) is provided with observation window (5) on seed culture tank (14) outer wall, is provided with below the observation window (5) Master station (12).
2. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the support base (15) it is fastened together with the seed culture tank (14) by screw, the pressure inductor (8) and the seed are trained It supports tank (14) to be fastened together by screw, pressure inductor (8) operating temperature range is -10 DEG C~80 DEG C.
3. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the filter (7) it is fastened together with the seed culture tank (14) by screw, the feed inlet (6) takes shape in the seed culture At the top of tank (14), filter (7) the filtering accuracy range is 20-75um.
4. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the temperature control plate (13) it is fastened together with the seed culture tank (14) by screw, the oxygen-supplying tube (11) and the temperature control plate (13) It is fastened together by screw, temperature control plate (13) adjustable range is -5 DEG C~50 DEG C.
5. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the temperature sense Device (10) is fastened together with the temperature control plate (13) by screw, and the exhaust valve (9) and the temperature control plate (13) are logical It crosses screw anchor to link together, the quantity of the exhaust valve (9) is 3.
6. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the quick coupling (3) it is fastened together with the seed culture tank (14) by screw, the pressure pump (4) and the seed culture tank (14) it is linked together by pipe collar.
7. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the stainless steel connects Adapter tube (17) is fastened together with the quick coupling (3) by screw, the steam sterilizer (2) and the stainless steel Connecting tube (17) is fastened together by screw.
8. a kind of device for continuously preparing amino acid strain according to claim 1, it is characterised in that: the seeding tank (1) it is fastened together with the quick coupling (3) by screw, the observation window (5) is embedded in the seed culture tank (14) on outer wall, the master station (12) is fastened together with the seed culture tank (14) by screw.
CN201821044312.2U 2018-07-03 2018-07-03 A kind of device continuously preparing amino acid strain Active CN208815010U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821044312.2U CN208815010U (en) 2018-07-03 2018-07-03 A kind of device continuously preparing amino acid strain

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821044312.2U CN208815010U (en) 2018-07-03 2018-07-03 A kind of device continuously preparing amino acid strain

Publications (1)

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Country Status (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669659A (en) * 2019-07-05 2020-01-10 青海华牛生物科技有限公司 Matrimony vine ferment feed liquid inoculation jar

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669659A (en) * 2019-07-05 2020-01-10 青海华牛生物科技有限公司 Matrimony vine ferment feed liquid inoculation jar

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