CN208803095U - Cell culture tube - Google Patents
Cell culture tube Download PDFInfo
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- CN208803095U CN208803095U CN201821426564.1U CN201821426564U CN208803095U CN 208803095 U CN208803095 U CN 208803095U CN 201821426564 U CN201821426564 U CN 201821426564U CN 208803095 U CN208803095 U CN 208803095U
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- cell culture
- bottom wall
- tube body
- accommodating chamber
- elastic piece
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Abstract
The utility model relates to a kind of cell culture tubes, transparent tube body and pipe cap assemblies including being in tubular structure open at one end.Pipe cap assemblies include lid and elastic piece.Lid includes bottom wall and is circumferentially arranged along bottom wall and the side wall that connect with bottom wall.Bottom wall and side wall, which enclose, to be set to form the accommodating chamber that one end has opening.Bottom wall offers the well through bottom wall, and the opening of well and accommodating chamber is oppositely arranged.One end of transparent tube body opening is removably contained in accommodating chamber.Elastic piece is accommodated and is fixed in accommodating chamber.The setting of elastic piece effectively reduces the time being loaded to cell culture tube, is effectively shortened the time of cell preparation.Moreover, the culture and centrifugation of the cell in culture solution can be completed in cell culture tube, therefore the step of transfer culture solution is eliminated between cell culture and centrifugation, further shorten the preparation time of cell.Therefore, the setting of above-mentioned cell culture tube, so that the working efficiency of cell preparation is higher.
Description
Technical field
The utility model relates to technical field of bioengineering, more particularly to a kind of cell culture tube.
Background technique
During cell preparation, the culture and centrifugation of cell carry out in culture bottle and centrifuge tube respectively.Mesh
Before, when cell harvests after the completion of culture in culture solution, culture solution can be transferred in centrifuge tube from culture bottle, while will training
The label supported on bottle is also transferred to centrifuge tube together.
But since cell culture needs to carry out in an aseptic environment, when culture solution is shifted from culture bottle, need to beat
The bottle cap of culture bottle and the pipe lid of centrifuge tube are opened, therefore the transfer need of work of culture solution is extremely careful, so the transfer of culture solution
The time needed is longer, so that cell preparation takes a long time.Further, the label on culture bottle is transferred to centrifuge tube
Movement, easily there is a situation where labeing mistake, therefore the process of label transfer needs continuous confirmation, further extends cell
The time of preparation leverages the working efficiency of cell preparation
Utility model content
Based on this, it is necessary to for the not high problem of the working efficiency of traditional cell preparation, provide a kind of working efficiency
High Tissue Culture Flask.
A kind of cell culture tube characterized by comprising
Transparent tube body, in tubular structure open at one end；
Pipe cap assemblies, comprising:
Lid, including bottom wall and the side wall for being circumferentially arranged along the bottom wall and being connect with the bottom wall, the bottom wall and institute
It states side wall and encloses and set to form the accommodating chamber that one end has opening, the bottom wall offers the well through the bottom wall, and described
The opening of well and the accommodating chamber is oppositely arranged, and one end of the transparent tube body opening is removably contained in the receiving
Chamber；
Elastic piece is accommodated and is fixed in the accommodating chamber.
The side chamber wall of the accommodating chamber offers card slot along the circumferential direction of the lid in one of the embodiments, described
Elastic piece is fastened in the card slot.
The card slot is rectangular channel in one of the embodiments,.
The surface of the elastic piece is bonded with the bottom wall in one of the embodiments,.
The outer wall of the transparent tube body open at one end is provided with external screw thread, the accommodating chamber in one of the embodiments,
Inner wall be provided with internal screw thread, the external screw thread and the screw-internal thread fit, so that the pipe cap assemblies are covered on the tube body.
The lateral surface of the lid is provided with skid resistance strip in one of the embodiments, and the skid resistance strip is described in
Lid it is axially extending.
The transparent tube body is provided with scale along its axial direction in one of the embodiments,.
The one end of the transparent tube body far from its opening is in truncated cone-shaped in one of the embodiments,.
The transparent tube body is polycarbonate tube body in one of the embodiments,.
The elastic piece is sheet rubber in one of the embodiments,.
Above-mentioned cell culture tube, when sample-adding, sample-adding syringe needle insertion well simultaneously pierces through elastic piece, can inject culture solution
In transparent tube body；After extracting syringe needle, elastic piece then returns to the state for sealing well by the elastic deformation performance of itself.By
This, elastic piece is accommodated and is fixed in accommodating chamber, so that cell culture tube is constantly in sealing state during sample-adding, and
And no longer need to open pipe lid sample-adding when being loaded to cell culture tube, the time being loaded to cell culture tube is greatly reduced, effectively
Ground shortens the time of cell preparation.And it can be directly centrifuged, after taking-up cell culture tube so that carefully in cell harvest
The culture and centrifugation of born of the same parents can be completed in cell culture tube, therefore eliminate transfer culture solution between the culture and centrifugation of cell
The step of, further shorten the preparation time of cell.Therefore, the setting of above-mentioned cell culture tube, so that the work of cell preparation
Make more efficient.
Detailed description of the invention
Fig. 1 is the explosive view of the cell culture tube in the utility model preferred embodiment；
Fig. 2 is the structural schematic diagram of the lid in cell culture tube shown in Fig. 1；
Fig. 3 is the cross-sectional view of lid shown in Fig. 2.
Specific embodiment
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms
It realizes, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes to the utility model
The understanding of disclosure is more thorough and comprehensive.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term " and or " used herein includes
Any and all combinations of one or more related listed items.
Also referring to Fig. 1, the cell culture tube 100 in the utility model preferred embodiment include transparent tube body 110 and
Pipe cap assemblies 120.Wherein, pipe cap assemblies 120 include lid 121 and elastic piece 122.
Transparent tube body 110 is in tubular structure open at one end.Transparent tube body 110 is for holding culture solution, to complete cell
Culture and centrifugation.For the ease of observation transparent tube body 110 in culture solution number and cell culture situation, transparent tube body
110 materials usually good by translucency such as glass, transparent plastics and good toughness are made.
Further, in the present embodiment, transparent tube body 110 is polycarbonate tube body.Polycarbonate (abbreviation PC plastic)
It is a kind of armorphous thermoplastic resin of high comprehensive performance, there is excellent ductility, dimensional stability, translucency, resistance toization
Corrosivity, higher intensity, preferable toughness, heat resistance and cold resistance are learned, therefore transparent tube body 110 also has good light transmission
The advantages that property, higher intensity, preferable toughness, chemical corrosion resistance, heat resistance, cold resistance.Therefore, by transparent tube body 110
Material be set as polycarbonate, significantly reduce transparent tube body 110 occur low temperature brittleness, high temperature melt, break into pieces, chemistry it is rotten
The probability of situations such as erosion substantially prolongs the service life of transparent tube body 110.
Further, in the present embodiment, transparent tube body 110 is provided with scale along its axial direction.It is needing to cell culture
When pipe 100 is loaded, it is only necessary to read calibration data while adding sample, so that it may fast and accurately complete sample-adding work.
Therefore, scale is set on transparent tube body 110, so that it is more convenient accurate to be loaded work to cell culture tube 100.
Please refer to figs. 2 and 3 together, and lid 121 is including bottom wall 1211 and along the circumferential setting of bottom wall 1211 and and bottom wall
The side wall 1212 of 1211 connections.Bottom wall 1211 and side wall 1212, which surround, forms the accommodating chamber 1213 that one end has opening.General feelings
Under condition, lid 121 is made of transparent plastic, aluminium plastic material etc..Bottom wall 1211 offers the well 1214 through bottom wall 1211,
And well 1214 and the opening of accommodating chamber 1213 are oppositely arranged.Well 1214 can be round hole, slotted eye, square hole
Or the hole of other shapes.Specifically in the present embodiment, well 1214 is round hole.
One end that transparent tube body 110 is open removably is contained in accommodating chamber 1213.Therefore, lid 121 and transparent pipe
Body 110 is detachably connected.Lid 121 mainly seals.Lid 121 can removably be pacified by being clamped, being spirally connected etc.
Dress mode is connect with transparent tube body 110, to guarantee the leakproofness of cell culture tube 100.Before cell preparation and after completing,
It requires that cell culture tube 100 is cleaned and sterilized, lid 121 and transparent tube body 110 are detachably connected, so that cell
The cleaning of culture tube 100 and disinfection are more convenient.
Further, in the present embodiment, one end that transparent tube body 110 is open is provided with external screw thread.Accommodating chamber 1213
Inner wall is provided with internal screw thread.External screw thread and screw-internal thread fit, so that pipe cap assemblies 120 are covered on transparent tube body 110.It covers as a result,
Body 121 is spirally connected with transparent tube body 110, while so that cell culture tube 100 uses more convenient, has better seal effect
Fruit.
Further, in the present embodiment, the lateral surface of lid 121 is provided with skid resistance strip 1215.And skid resistance strip
1215 along the axially extending of lid 121.It is all to grasp lid 121 by manpower come real that the opening and lid of cell culture tube 100, which are closed,
Existing.Open or cover close cell culture tube 100 during, the setting of skid resistance strip 1215, considerably increase manpower with
The frictional force of 121 lateral wall of lid, in order to turn lid 121, so that the opening of cell culture tube 100 and lid conjunction are more square
Just.
Elastic piece 122 is accommodated and is fixed in accommodating chamber 1213.When pipe cap assemblies 120 are covered on transparent tube body 110, bullet
Property piece 122 is located at bottom wall 1211 and transparent tube body 110 between the end face of its open at one end.Elastic piece 122 can be by viscous
It the modes such as connects, be clamped to be fixed in accommodating chamber 1213.Elastic piece 122 is fixed in accommodating chamber 1213, so that cell culture tube
100 better tightness.
Further, in the present embodiment, elastic piece 122 is sheet rubber.Since rubber is a kind of with reversible deformation
High elastic polymer material has the characteristics such as elasticity is good, intensity height, good combination property, chemical stability are good.So elastic piece
122 also with elasticity is good, intensity height, good combination property, good chemical stability.Therefore, rubber is set by elastic piece 122
Film, so that the elastically deformable performance of elastic piece 122 is more preferable, so that the better tightness of cell culture tube 100.
Elastic piece 122 is accommodated and is fixed in accommodating chamber 1213, so that cell culture tube 100 is before guaranteeing leakproofness
Putting keeps sample-adding work more convenient, substantially reduces the loading time of cell culture tube 100.Moreover, being harvested in cell
When take out cell culture tube 100 after, can be directly centrifuged, eliminate transfer culture solution the step of, substantially reduce cell
Preparation time.Therefore, the setting of above-mentioned cell culture tube 100, so that the working effect of cell preparation is higher.
In the present embodiment, the side chamber wall of accommodating chamber 1213 offers card slot 1216 along the circumferential direction of lid 121.Card slot 1216
Shape can be rectangle, arc, taper or other shapes.Elastic piece 122 is fastened in card slot 1216.Card slot 1216 is set
It sets, as long as so that elastic piece 122 is caught in the installation that elastic piece 122 can be completed in card slot 1216, therefore the setting of card slot 1216 makes
It is more convenient to obtain installation of the elastic piece 122 on lid 121.And elastic piece 122 is fastened in card slot 1216, so that elastic piece
122 is more secured with the connection of lid 121.
Further, in the present embodiment, card slot 1216 is rectangular channel.Card slot 1216 is shaped to rectangle, is made
Obtaining has biggish contact area between elastic piece 122 and card slot 1216, so that the connection of elastic piece 122 and lid 121
It is relatively firm, to be effectively guaranteed the leakproofness of cell culture tube 100.
Further, in the present embodiment, the surface of elastic piece 122 is bonded with bottom wall 1211.Work as bottle cap component as a result,
120 when being covered on transparent tube body 110, and elastic piece 122 is held on end face and bottom wall of the transparent tube body 110 close to its open at one end
Between 1211, fixed function is further served as to elastic piece 122, so that elastic piece 122 and the connection of lid 121 are more secured, into
And make the better tightness of cell culture tube 100.
In the present embodiment, the one end of transparent tube body 110 far from its opening is in truncated cone-shaped.So transparent tube body 110 is separate
The side wall of its open at one end has taper.Cell after centrifugation is all deposited in the bottom of transparent tube body 110 substantially, and sees after being centrifuged
It needs to tilt cell culture tube 100 when examining cell quantity, therefore sets rotary table far from one end of its opening for transparent tube body 110
Shape, so that the cell precipitation after centrifugation is observed on the side wall that transparent tube body 110 has taper far from its open at one end with facilitating
Cell concentration after centrifugation.
In addition, one end due to transparent tube body 110 far from its opening is in truncated cone-shaped, then transparent tube body 110 is far from its opening
The end face of one end is plane.Plane is set far from the end face of its open at one end by transparent tube body 110, to thin when can prevent from being centrifuged
The excessive friction of born of the same parents, and then can reduce because of situations such as excessive friction and cause the serious probability occurred of cell damage, so that carefully
The reliability of born of the same parents' preparation is higher.
Above-mentioned cell culture tube 100, when sample-adding, sample-adding syringe needle insertion well 1214 simultaneously pierces through elastic piece 122, can will
Culture solution injects in transparent tube body 110；After extracting syringe needle, elastic piece 122 then returns to envelope by the elastic deformation performance of itself
The firmly state of well 1214.Elastic piece 122 is accommodated as a result, and is fixed in accommodating chamber 1213, so that cell culture tube 100
It no longer needs to open the sample-adding of lid 121 when being constantly in sealing state during sample-adding, and being loaded to cell culture tube 100,
The time being loaded to cell culture tube 100 is greatly reduced, the time of cell preparation is effectively shortened.And it is harvested in cell
When take out cell culture tube 100 after, can be directly centrifuged so that cell culture and centrifugation can be in cell culture tube 100
Interior completion, therefore the step of transfer culture solution is eliminated between the culture and centrifugation of cell, further shorten the preparation of cell
Time.Therefore, the setting of above-mentioned cell culture tube 100, so that the working efficiency of cell preparation is higher.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (10)
1. a kind of cell culture tube characterized by comprising
Transparent tube body, in tubular structure open at one end；
Pipe cap assemblies, comprising:
Lid, including bottom wall and the side wall for being circumferentially arranged along the bottom wall and being connect with the bottom wall, the bottom wall and the side
Wall, which encloses, to be set to form the accommodating chamber that one end has opening, and the bottom wall offers the well through the bottom wall, and the sample-adding
Hole and the opening of the accommodating chamber are oppositely arranged, and one end of the transparent tube body opening is removably contained in the accommodating chamber；
Elastic piece is accommodated and is fixed in the accommodating chamber.
2. cell culture tube according to claim 1, which is characterized in that the side chamber wall of the accommodating chamber is along the lid
Card slot circumferentially is offered, the elastic piece is fastened in the card slot.
3. cell culture tube according to claim 2, which is characterized in that the card slot is rectangular channel.
4. cell culture tube according to claim 2, which is characterized in that the surface of the elastic piece and the bottom wall paste
It closes.
5. cell culture tube according to claim 1, which is characterized in that the outer wall of the transparent tube body open at one end is arranged
There is external screw thread, the inner wall of the accommodating chamber is provided with internal screw thread, the external screw thread and the screw-internal thread fit, so that the pipe lid
Component is covered on the tube body.
6. cell culture tube according to claim 5, which is characterized in that the lateral surface of the lid is provided with tread plate
Line, the skid resistance strip are axially extending along the lid.
7. cell culture tube according to claim 1, which is characterized in that the transparent tube body is provided with quarter along its axial direction
Degree.
8. cell culture tube according to claim 1, which is characterized in that the one end of the transparent tube body far from its opening is in
Truncated cone-shaped.
9. cell culture tube according to claim 1, which is characterized in that the transparent tube body is polycarbonate tube body.
10. cell culture tube according to claim 1, which is characterized in that the elastic piece is sheet rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821426564.1U CN208803095U (en) | 2018-08-31 | 2018-08-31 | Cell culture tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821426564.1U CN208803095U (en) | 2018-08-31 | 2018-08-31 | Cell culture tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208803095U true CN208803095U (en) | 2019-04-30 |
Family
ID=66236827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821426564.1U Active CN208803095U (en) | 2018-08-31 | 2018-08-31 | Cell culture tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208803095U (en) |
-
2018
- 2018-08-31 CN CN201821426564.1U patent/CN208803095U/en active Active
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