CN208725934U - A kind of fixed device of living animal micro-imaging - Google Patents
A kind of fixed device of living animal micro-imaging Download PDFInfo
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- CN208725934U CN208725934U CN201721149538.4U CN201721149538U CN208725934U CN 208725934 U CN208725934 U CN 208725934U CN 201721149538 U CN201721149538 U CN 201721149538U CN 208725934 U CN208725934 U CN 208725934U
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Abstract
The utility model discloses a kind of fixed device of living animal micro-imaging, including bottom plate, fixed station, adsorption plate, fixed station is connect with bottom plate, cavity is equipped at the top of fixed station, several segmentations are equipped in cavity, the lower section of several segmentations is equipped with adjusting stud, stud is adjusted to connect with base thread, stud upper end is adjusted to contact with segmentation bottom, it is equipped at the top of fixed station along fixed station groove in the front-back direction, groove runs through the top of several segmentations, the front end of fixed station and the rear end of fixed station, texturearunaperpendicular is in several segmentations, adsorption plate is connect with fixed station top slide, first through hole is equipped at the top of adsorption plate, first through hole is located at the top of cavity, the side wall of first through hole is equipped with several ventholes, several ventholes are connect with negative pressure device, adsorption plate upper surface is equipped with coverslip, first through hole is located at below coverslip；The utility model can obtain the micro-image that stability is high, clarity is high.
Description
Technical field
The utility model relates to medical domain more particularly to a kind of fixed devices of living animal micro-imaging.
Background technique
It usually needs to carry out zoopery in modern biology and medical research, the zoopery of the past is all will be at animal
After death, the tissue for removing animal is just observed after handling tissue with plurality of reagents, the loss of energy and can be changed in this way
Therefore, under condition of living organism truth under animal survival state observes the vital movement in animal tissue, would be even more beneficial to me
Understand true vital movement process.But under condition of living organism, due to the breathing of animal, heartbeat, pulse and some tissues
Non-autonomous wriggling, contracture make object observing be difficult to stablize in a position (on tri- directions X, Y, Z all fixed), Z-direction
On displacement fix target can not to the distance of micro objective, so being difficult to obtain clearly image；X Y direction shifting
Position will lead to scalloping, hangover；And the visual field can not be fixed, then and between different samples, comparativity is poor between different time points.By
It is substantially reduced in the repeatability of above-mentioned reason, living animal experiment.Existing living animal micro imaging method is difficult to solve
The above problem can not observe the biological phenomena of cellular level especially for the heart, the organ of this strenuous exercise of lung completely.
Utility model content
The utility model is intended to provide a kind of fixed device of living animal micro-imaging, for solving in the prior art because dynamic
Micro-image stability caused by object organ movement is low, poor definition, the problem of cannot observing for a long time, to widen significantly
Living animal tests enforceable experiment type, improves observation depth and precision, and have good repeatability, greatly
Improve the accuracy and confidence level of research.
In order to achieve the above objectives, the technical solution adopted in the utility model is as follows: a kind of living animal micro-imaging is fixed
Device, including bottom plate, fixed station, adsorption plate, the fixed station bottom are connect with plate upper surface, and sky is equipped at the top of fixed station
Chamber, cavity is interior to be equipped with several segmentations, and lower sections of several segmentations are equipped with adjusting stud, adjust stud and base thread
Connection adjusts stud upper end and contacts with segmentation bottom, described equipped with along fixed station groove in the front-back direction at the top of the fixed station
Groove runs through top, the front end of fixed station and the rear end of fixed station of several segmentations, and texturearunaperpendicular is described in several segmentations
Adsorption plate is connect with fixed station top slide, and absorption plate surface is equipped with first through hole, and first through hole is located at the top of cavity, and first
The side wall of through-hole is equipped with several ventholes, several described ventholes are connect with negative pressure device, and adsorption plate upper surface is equipped with
Coverslip, first through hole are located at below coverslip.
Further, two sliding slots parallel to each other are additionally provided at the top of the fixed station, the groove is located at two sliding slots
Between, groove is parallel to sliding slot, is equipped with sliding block in two sliding slots, and upper end of slide block is threadedly coupled with positioning stud, the absorption
The left and right ends of plate are symmetrically equipped with open slot, and the first through hole is located between two open slots, and it is fixed that opening groove depth is greater than
Position stud diameter, opening groove width are adapted with positioning stud diameter, less than two cunnings of the distance between two opening trench bottoms
The distance between slot, positioning stud top pass through open slot and connect with location nut.
Preferably, two sliding slots are symmetrically set to the two sides of the groove, and groove is arc.
The bottom plate is threadedly coupled with fixed station, and bottom plate is equipped with several threaded holes, and the adjusting stud and threaded hole connect
It connects, the fixed station both ends are symmetrically set there are four the second through-hole, and fixed bolt upper end passes through threaded hole, the second through-hole and fixation
Nut connection, fixed station top are additionally provided with counterbore compatible with the fixture nut shape, and fixture nut is located at the counterbore
Interior, counterbore depth is not less than fixture nut height, and fixed bolt is adapted with the second through-hole.
Preferably, the length of the fixed bolt is greater than the sum of height and thickness of bottom plate of fixed station.
Preferably, the fixed station is rectangle, and the cavity is rectangle, and the bottom of cavity left and right sidewall is equipped with boss,
Described several are segmented into cuboid.
Further, the length of the segmentation left and right directions is adapted with the spacing of cavity left and right sidewall, the height of segmentation
It is not more than the height of fixed station with the sum of the height of boss, the adjustings stud is located at segmentation underface.
Preferably, there are five the segmentation is set, spacing of the sum of the five segmentation front-rear direction length with cavity front-rear side walls
It is adapted.
Preferably, there are four the ventholes, four ventholes are uniformly set on the same circumference of first through hole side wall.
Preferably, the venthole is connect by snorkel with negative pressure device, and snorkel one end connects negative pressure device, ventilation
The side wall that the pipe other end passes through adsorption plate is connected to venthole.
Preferably, the adsorption plate, fixed station are the low material of thermal coefficient.
The utility model has the following beneficial effects:
1, arc groove, cavity, several segmentations in cavity, the setting of the adjusting screw rod immediately below segmentation, make animal
Can be stable lie in groove, and can be adjusted according to the needs the height of different parts, enable the tissue for needing to observe
It is contacted with adsorption plate；
2, the setting of first through hole, venthole, coverslip and negative pressure device enables animal blood vessels tissue to be adsorbed, is solid
It is scheduled at first through hole, micro-image is made to keep stablizing, clean；
3, it is uniformly distributed in the setting of four ventholes on the same circumference of first through hole side wall, is conducive to vascular tissue and stablizes
At the first through hole center, facilitate micro- sem observation；
4, two sliding slots parallel to each other, the setting of the sliding block, positioning stud and open slot of threaded connection, make to adsorb
Plate can be before and after fixed station and left and right directions slides, and is conducive to carry out micro-imaging to different parts vascular tissue；
5, the setting of counterbore and the fixture nut in counterbore makes adsorption plate not will receive the limit of location nut when sliding
System；
6, on bottom plate several threaded holes setting, so that fixed station is had different installation sites, can also use
In fixing other experiment connecting tubes；
7, adsorption plate, fixed station made of low thermal conductivity material, can effectively reduce the reduction of experimental animal body temperature, subtract
Few influence because of hypothermia to image tissue, improves the confidence level of micro-imaging result.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is fixed station sliding slot and positioning stud schematic diagram；
Fig. 3 is adsorption plate structural schematic diagram；
Fig. 4 is fixed station and bottom plate connection schematic diagram；
Fig. 5 is the utility model assembling schematic diagram.
In figure: 1- bottom plate, 2- fixed station, 3- adsorption plate, 11- threaded hole, 20- counterbore, 21- groove, 22- segmentation, 23-
Second through-hole, 24- fixture nut, 25- fixed bolt, 26- sliding slot, 27- sliding block, 28- positioning stud, 29- location nut, 31-
First through hole, 32- snorkel, 33- open slot, 311- venthole, 221- adjust stud.
Specific embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing, to this reality
It is further elaborated with novel.
As shown in Figure 1, a kind of fixed device of living animal micro-imaging, including bottom plate 1, fixed station 2, adsorption plate 3, it is described
Adsorption plate 3 and fixed station 2 are that the low material of thermal coefficient is made or is insulated material and is made, such as adsorption plate 3 and fixed station 2
For materials such as plastics, timber.As shown in figure 4, the bottom plate 1 is equipped with several threaded holes 11, the fixed station 2 is rectangle, Gu
The quadrangle for determining platform 2 is equipped with the second through-hole 23, and the top of the second through-hole 23 is additionally provided with counterbore 20, and counterbore 20 and the second through-hole 23 are total
Axis, counterbore 20 is interior to be equipped with fixture nut 24, and the depth of counterbore 20 is equal to the thickness of fixture nut 24, and fixture nut 24 is hexagonal
The shape of nut, counterbore 20 is adapted with fixture nut 24, and bottom plate 1 is connect with fixed station 2 by fixed bolt 25, fixed bolt
25 upper end is connect after passing through threaded hole 11, the second through-hole 23 with fixture nut 24, and the top of fixed bolt 25, which is no more than, to be fixed
The length of the upper surface of platform 2, fixed bolt 25 is greater than the height of fixed station 2 and the sum of the thickness of bottom plate 1.
As shown in Fig. 2, the top of fixed station 2 is equipped with rectangular enclosure, rectangular enclosure includes open top, rectangular enclosure or so
The bottom of side wall is equipped with boss along the longitudinal direction, is set in rectangular enclosure there are five segmentation 22, and segmentation 22 is cuboid, segmentation
The length of 22 left and right directions is adapted with the spacing of rectangular cavity left and right sidewall, the sum of five 22 front-rear direction length of segmentation and rectangle
The spacing of cavity front-rear side walls is adapted, and is segmented the height that the sum of 22 height and the height of boss are less than fixed station 2.Described five
The lower section of a segmentation 22, which is equipped with, adjusts stud 221, adjusts stud 221 and connect with the threaded hole 11 of bottom plate 1, adjusts stud 221
Upper end contacted with the bottom of segmentation 22, the lower end for adjusting stud 221 is located at the lower section of bottom plate 1.It is equipped at the top of the fixed station 2
Along the groove 21 of the arc in the front-back direction of fixed station 2, the groove 21 is before the top of five segmentations 22, fixed station 2
The rear end at end and fixed station 2, groove 21 is perpendicular to five segmentations 22.
As shown in figure 3,3 surface of adsorption plate be equipped with first through hole 31, the side wall of first through hole 31 set there are four uniformly
The venthole 311 of distribution, four ventholes 311 are uniformly set on the same circumference of 31 side wall of first through hole, venthole
311 are connect by snorkel 32 with negative pressure device, and one end of snorkel 32 connects negative pressure device, and the other end of snorkel 32 is worn
The side wall for crossing adsorption plate 3 is connected to venthole 311, and the upper surface of adsorption plate 3 is additionally provided with coverslip, and first through hole 31 is located at lid
Below slide, snorkel 32 is arranged in a staggered manner with groove 21.
As shown in figure 5, the top of the fixed station 2 is additionally provided with two sliding slots 26 parallel to each other, the groove 21 is located at
The centre of two sliding slots 26, two sliding slots 26 are symmetrically set to the two sides of groove 21, and groove 21 is parallel to sliding slot 26, two sliding slots
Sliding block 27 is equipped in 26, sliding slot 26 is T-slot, and sliding block 27 is T-block, and the upper end of sliding block 26 and 28 screw thread of positioning stud connect
It connects, the left and right ends of the adsorption plate 3 are symmetrically equipped with open slot 33, and the first through hole 31 is located in two open slots 33
Between, the depth of open slot 33 is greater than the diameter of positioning stud 28, and the width of open slot 33 is mutually fitted with the diameter of positioning stud 28
Match, less than the distance between two sliding slots 26, the top of positioning stud 28, which passes through, is opened for the distance between two 33 bottoms of open slot
Mouth slot 33 is connect with location nut 29.
In use, the animal of experiment made on the living is placed in arc groove 21, adsorption plate 3 is placed on table on fixed station 2
Adsorption plate 3 is slided in face, then front and rear, left and right, makes the tissue face first through hole 31 for needing to carry out micro-imaging, screwed in place nut
29 keep adsorption plate 3 fixed, and coverslip is placed on 31 top of first through hole, and coverslip is fixed on adsorption plate 3 by water seal method,
Adjustment adjusts stud 211, makes that the vascular tissue for carrying out micro-imaging or animal tissue is needed to contact with adsorption plate 3, will be micro-
Mirror is directed at the tissue for needing to carry out micro-imaging, opens negative pressure device and needs to carry out the group of micro-imaging under the action of negative pressure
Knitting can be attracted in first through hole 31, and the group being adsorbed at this time, which is woven on tri- directions X, Y, Z, will all keep stablizing, it will
The micro-image high to stability, clarity is high.
Certainly, the utility model can also have other various embodiments, without departing substantially from the spirit of the present invention and its essence
In the case of, those skilled in the art can make various corresponding changes and modifications, but these phases according to the utility model
The change and modification answered all should belong to the protection scope of the utility model the attached claims.
Claims (10)
1. a kind of fixed device of living animal micro-imaging, it is characterised in that: including bottom plate (1), fixed station (2), adsorption plate
(3), fixed station (2) bottom is connect with bottom plate (1) upper surface, and cavity is equipped at the top of fixed station (2), is equipped in cavity several
A segmentation (22), described several, which are segmented to be equipped with below (22), adjusts stud (221), adjusts stud (221) and bottom plate (1)
It is threadedly coupled, adjusts stud (221) upper end and contacted with segmentation (22) bottom, is equipped at the top of the fixed station (2) along fixed station (2)
Groove (21) in the front-back direction, the groove (21) is through the top of several segmentations (22), the front end of fixed station (2) and fixation
The rear end of platform (2), groove (21) are segmented (22) perpendicular to several, sliding at the top of adsorption plate (3) lower surface and fixed station (2)
Dynamic connection, adsorption plate (3) surface are equipped with first through hole (31), and first through hole (31) is located at the top of cavity, first through hole (31)
Side wall be equipped with several ventholes (311), several described ventholes (311) are connect with negative pressure device, on adsorption plate (3)
Surface is additionally provided with coverslip, and first through hole (31) is located at below coverslip.
2. the fixed device of a kind of living animal micro-imaging according to claim 1, it is characterised in that: the fixed station
(2) top is additionally provided with two sliding slots (26) parallel to each other, and the groove (21) is located between two sliding slots (26), groove (21)
It is parallel to sliding slot (26), is equipped with sliding block (27) in two sliding slots (26), sliding block (27) upper end and positioning stud (28) screw thread connect
It connects, the left and right ends of the adsorption plate (3) are symmetrically equipped with open slot (33), and the first through hole (31) is located at two open slots
(33) between, open slot (33) depth is greater than positioning stud (28) diameter, open slot (33) width and positioning stud (28) diameter
It is adapted, the distance between less than two sliding slots (26) in the distance between two open slot (33) bottoms, positioning stud (28) top
End is connect across open slot (33) with location nut (29).
3. the fixed device of a kind of living animal micro-imaging according to claim 2, it is characterised in that: two sliding slots
(26) two sides of the groove (21) are symmetrically set to, groove (21) is arc.
4. the fixed device of a kind of living animal micro-imaging according to claim 1, it is characterised in that: the bottom plate (1)
It is threadedly coupled with fixed station (2), bottom plate (1) is equipped with several threaded holes (11), the adjustings stud (221) and threaded hole (11)
Connection, fixed station (2) top are equipped with the second through-hole (23), and fixed bolt (25) upper end leads to across threaded hole (11), second
Hole (23) is connect with fixture nut (24), and it is compatible with the fixture nut (24) shape heavy to be additionally provided at the top of fixed station (2)
Hole, fixture nut (24) are located in the counterbore, and counterbore depth is not less than fixture nut (24) height, fixed bolt (25) and the
Two through-holes (23) are adapted.
5. the fixed device of a kind of living animal micro-imaging according to claim 2, it is characterised in that: the sliding slot (26)
For T-slot, the sliding block (27) is T-block or hexagon block.
6. the fixed device of a kind of living animal micro-imaging according to claim 1, it is characterised in that: the fixed station
It (2) is rectangle, the cavity is rectangle, and the bottom of cavity left and right sidewall is equipped with boss, and the segmentation (22) is cuboid, point
There are five sections (22), and the length of segmentation (22) left and right directions is adapted with the spacing of cavity left and right sidewall, before five segmentations (22)
The sum of length of rear direction is adapted with the spacing of cavity front-rear side walls, is segmented the sum of the height of (22) and the height of boss less
Height in fixed station (2), the adjusting stud (221) are located at immediately below segmentation (22).
7. the fixed device of a kind of living animal micro-imaging according to claim 4, it is characterised in that: second through-hole
(23) there are four, four the second through-holes (23) are symmetrically set to the both ends of fixed station (2).
8. the fixed device of a kind of living animal micro-imaging according to claim 4, it is characterised in that: the fixed bolt
(25) length is greater than the height of fixed station (2) and the sum of the thickness of bottom plate (1).
9. the fixed device of a kind of living animal micro-imaging according to claim 1, it is characterised in that: the venthole
(311) there are four, four ventholes (311) are uniformly set on the same circumference of first through hole (31) side wall, venthole (311)
It is connect by snorkel (32) with negative pressure device, snorkel (32) one end connects negative pressure device, and snorkel (32) other end passes through
The side wall of adsorption plate (3) is connected to venthole (311), and snorkel (32) is arranged in a staggered manner with groove (21).
10. the fixed device of a kind of living animal micro-imaging according to claim 1, it is characterised in that: the adsorption plate
(3), fixed station (2) is the low material of thermal coefficient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721149538.4U CN208725934U (en) | 2017-09-08 | 2017-09-08 | A kind of fixed device of living animal micro-imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721149538.4U CN208725934U (en) | 2017-09-08 | 2017-09-08 | A kind of fixed device of living animal micro-imaging |
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| Publication Number | Publication Date |
|---|---|
| CN208725934U true CN208725934U (en) | 2019-04-12 |
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| CN201721149538.4U Active CN208725934U (en) | 2017-09-08 | 2017-09-08 | A kind of fixed device of living animal micro-imaging |
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| CN110286016A (en) * | 2019-07-11 | 2019-09-27 | 云南大学 | A kind of living insects feeler directed fixing device in irregular shape and preparation method thereof |
| CN111012524A (en) * | 2019-12-27 | 2020-04-17 | 苏州溢博伦光电仪器有限公司 | Separated optical vibration reduction platform |
| CN111024934A (en) * | 2019-12-27 | 2020-04-17 | 苏州溢博伦光电仪器有限公司 | Vibration reduction type microscopic observation method |
| CN111419181A (en) * | 2020-03-31 | 2020-07-17 | 浙江大学 | Device for long-time living body imaging of small animal and using method thereof |
| CN113679338A (en) * | 2020-05-18 | 2021-11-23 | 北京生命科学研究所 | Animal live body imaging fixing device |
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2017
- 2017-09-08 CN CN201721149538.4U patent/CN208725934U/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286016A (en) * | 2019-07-11 | 2019-09-27 | 云南大学 | A kind of living insects feeler directed fixing device in irregular shape and preparation method thereof |
| CN110286016B (en) * | 2019-07-11 | 2023-08-04 | 云南大学 | Irregular antenna directional fixing device for living fruit fly insects and manufacturing method thereof |
| CN111012524A (en) * | 2019-12-27 | 2020-04-17 | 苏州溢博伦光电仪器有限公司 | Separated optical vibration reduction platform |
| CN111024934A (en) * | 2019-12-27 | 2020-04-17 | 苏州溢博伦光电仪器有限公司 | Vibration reduction type microscopic observation method |
| CN111024934B (en) * | 2019-12-27 | 2021-12-07 | 苏州溢博伦光电仪器有限公司 | Vibration reduction type microscopic observation method |
| CN111419181A (en) * | 2020-03-31 | 2020-07-17 | 浙江大学 | Device for long-time living body imaging of small animal and using method thereof |
| CN111419181B (en) * | 2020-03-31 | 2023-07-25 | 浙江大学 | Device for long-time living body imaging of small animals and application method thereof |
| CN113679338A (en) * | 2020-05-18 | 2021-11-23 | 北京生命科学研究所 | Animal live body imaging fixing device |
| CN113679338B (en) * | 2020-05-18 | 2023-07-18 | 北京生命科学研究所 | Animal live body formation of image fixing device |
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