CN110286016A - A kind of living insects feeler directed fixing device in irregular shape and preparation method thereof - Google Patents
A kind of living insects feeler directed fixing device in irregular shape and preparation method thereof Download PDFInfo
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- CN110286016A CN110286016A CN201910625649.5A CN201910625649A CN110286016A CN 110286016 A CN110286016 A CN 110286016A CN 201910625649 A CN201910625649 A CN 201910625649A CN 110286016 A CN110286016 A CN 110286016A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The present invention discloses a kind of living insects feeler directed fixing device in irregular shape and preparation method thereof, belongs to laboratory sample processing technology field.The device includes glass slide, feeler pad, polypide pad, water storage The Small Well device, ultraviolet glue, water storage The Small Well device is fixed at the middle part of glass slide, feeler pad is arranged on glass slide and feeler pad is located in water storage The Small Well device, polypide pad is arranged on glass slide and polypide pad is located in water storage The Small Well device, polypide pad is located at feeler pad side and contacts with feeler pad side, adhesive-layer is arranged in polypide pad upper surface, and coating is provided with ultraviolet glue on the junction and feeler pad of feeler pad and polypide pad.The present invention passes through the integration realization using multiple devices to the directional at-tachment of feeler in irregular shape, and convenient for single sensor observation, positioning and record, experimental efficiency is high, low in cost, reusable.
Description
Technical field
The present invention relates to a kind of directed fixing devices and preparation method thereof of living insects feeler in irregular shape, belong to reality
Test sample treatment technical field.
Background technique
Feeler is the important sense organ of insect.Insect feeler surface dense distribution various types of micron order sensors,
Single sensor is the basic structural unit of Insect Antennal Sensilla official, is the information that insect body perceives external environment, communicated
Reception device can experience physical mechanical or chemical stimulation.Insect is main during seeking spouse, finding host or prey habitat
If extra-sexual hormone, host plant volatile, sound and air etc. perceived chemically and physically by Antennal Sensilla official stimulate come
It completes, it is insect is looked for food, host identifies, is laid eggs, is mated, is hidden in the action processes such as enemy and is played an important role.For example,
The mustard oil odor-sensitive that cabbage butterfly (Pieris rapae) mediates the air that crucifer distributes.Many insects can quilt
The sour-sweet wine flavour that damage fruit is distributed is lured.The sex hormone of the female adult secretion of many moth class can attract the male worm outside km
Sudden mating.Therefore, the detected by scent of chemical substance observes and predicts pest population dynamics using insect feeler, to evil
Worm trapped, mass trapping etc..
The different sensory receptors neuron of function is distributed with inside antennal sensilla.By taking smell sensor as an example, extraneous smell object
Matter passes through sensor surfaces nano grade pore, identifies that transportational process eventually arrives at Olfactory receptor neurons by series, the final external worldization
It learns signal and is converted into electric signal, reach insect cerebral central nervous system so as to cause insect lipids reaction.Study insect touching
The electrophysiologic response of the sensory a certain seed type list sensor in angle, for understanding insect to change from deeper microcosmic point
Learn the information coding rule of smell and mechanical stimulus, ecological behavior is of great significance.
Now for the technique for fixing of the more regular feeler (feeler of model insects drosophila is flat) of form, usually adopt
Polypide is fixed with the pipette tips of micro sample adding appliance, is then carried out with the back side that flat feeler on coverslip is pushed down at glass electrode tip
It is fixed.Therefore, the sensor in back side direction can only be exposed, record, single sensor experiment in other directions can not achieve；For very small
Insect (mosquito) feeler fixing means it is then more simple, polypide and feeler are directly adhered to double-sided adhesive and are pressed in hair
On polypide and double-sided adhesive.
But some insects, such as Tephritidae insect, polypide and feeler size are larger, and cuticula is thicker, and translucency is lower；
It is irregular that feeler respectively saves form, and majority is ellipse；Feeler respectively saves thickness difference, and height difference is different；On feeler sensor length between
5-20 microns；A plurality of types of sensors often intersect dense distribution, and only 2-3 micron of the distance between single sensor, identification and
Location difficulty.It is anti-to the electro physiology of different physics and chemical stimulation in order to study different type sensor on Tephritidae insect feeler
Answer, need using living insects, need to differentiate at least under 1000 enlargement ratios with positioning sensor, and by the note at 1 micron of tip
Record electrode is accurately inserted into certain types of single sensor base portion (single sensor record experiment).Also, feeler cannot be sent out during recording
Raw slight mobile, otherwise electrode displacement will lead to target electric signal loss.It is therefore desirable to which living insects and feeler are carried out simultaneously
It is fixed.More importantly, it is contemplated that insect feeler is divided into back side, veutro, outside and interior direction, sensor Spatial Distribution Pattern
Difference, function is different, is the working method of comprehensive understanding feeler, needs to realize single sensor record of each angle, needs
To be directed to the directional at-tachment of irregular some angle of feeler of living insects form.In addition, in order to differentiate and be accurately positioned single sense
Device must use × 100 hydroscopes in the presence of water, observe various types of single sensors, and the micro-electrodes such as tungsten wire are passed through
The base portion of certain class sensor is inserted into micromanipulation, therefore while fixed polypide and feeler, palpus special designing insect shape is not advised
Then feeler directed fixing device.
Summary of the invention
The purpose of the present invention is to provide a kind of living insects feeler directed fixing device in irregular shape and its production sides
The quick and synchronous fixation to insect body and form irregular feeler can be achieved in method, the present invention, especially can be according to single sensor
The position of experiment requires, and realizes the directional at-tachment to the special angle of irregular feeler, is satisfied with single sense of more highly difficult rank
Device test micromanipulation, solve special angle is fixed, feeler cuticula is thicker, light transmittance is low, recording electrode puncture easily lead to
Target movement and single only 2-3 microns of spacing of sensor, the problem of exact localization operation hardly possible.
The present invention passes through the integration realization using multiple devices to the directional at-tachment of feeler in irregular shape, convenient for single sense
Device observation, positioning and record, experimental efficiency is high, low in cost, reusable.
A kind of insect feeler directed fixing device in irregular shape, including glass slide 1, feeler pad 6, polypide pad 3, storage
Water The Small Well device 2, ultraviolet glue 5, water storage The Small Well device 2 are fixed at the middle part of glass slide 1, and the setting of feeler pad 6 is carrying glass
On piece 1 and feeler pad 6 is located in water storage The Small Well device 2, and polypide pad 3 is arranged on glass slide 1 and polypide pad 3 is located at storage
In water The Small Well device 2, polypide pad 3 is located at 6 side of feeler pad and contacts with 6 side of feeler pad, 3 upper surface of polypide pad
Adhesive-layer 7 is set, and coating is provided with ultraviolet glue 5 on the junction and feeler pad 6 of feeler pad 6 and polypide pad 3.
The structure of the water storage The Small Well device 2 is annulus column or hollow polygon column.
Nearly feeler pad one end of the adhesive-layer 7 is less than the 1/2 of insect head length away from the distance of feeler pad 6, glues
The width and 3 equivalent width of polypide pad of glue-line 7.The height of the water storage The Small Well device 2 is 2~3mm.
The width of the ultraviolet glue 5 is greater than polypide head width.
It preferably, further include polypide fixing line 4, one end of polypide fixing line 4 is fixed at the adhesive-layer of polypide pad 3
On 7；Polypide fixing line 4 can preferably fix polypide, avoid living insects from struggling and be displaced.
Preferably, polypide fixing line 4 is two, is located at the cephalothorax merogenesis and chest and abdomen merogenesis of insect.
Preferably, the length of feeler pad 6 is 5~20mm, and it is highly 1~2mm that width, which is 3~5mm,；Polypide pad 3
Length is 10~25mm, and it is highly 0.5~1mm that width, which is 8~20mm,.
Preferably, the material of The Small Well device is hot melt adhesive.
Preferably, feeler pad 6 is glass slide, and polypide pad 3 is coverslip.
Preferably, adhesive-layer 7 is double-sided adhesive；
Feeler pad is capable of providing the pedestal of feeler directional at-tachment, facilitates the height difference for coordinating feeler and polypide, meets light transmission
Property；Polypide pad places polypide, fills up the difference in height of body and feeler；Adhesive-layer (double-sided adhesive) in polypide pad can be reduced
Insect body is struggled, the polypide fixing line being arranged in polypide pad, and the fixed line end fixation of polypide is sticked to adhesive-layer (double-sided adhesive)
On, polypide fixing line is pressed respectively against at cephalothorax merogenesis and chest and abdomen merogenesis, further fixes polypide；Feeler pad and polypide pad
Junction and feeler pad on ultraviolet glue can the fixed feeler of slewing and living insects head, feeler knot will not be destroyed
Structure and function；The Small Well device is water storing medium, it can be ensured that antennal sensilla is determined under 1000 times of amplifications by water object lens (× 100)
Type is accurately positioned, and electrode is properly inserted specific sensor with micromanipulative technique.
The production method of living insects feeler directed fixing device in irregular shape, the specific steps are as follows:
(1) glass slide is cut into feeler pad, the bottom surface of feeler pad is adhered in the middle part of the glass slide of another cleaning；
(2) coverslip is cut into polypide pad, the bottom surface of polypide pad is adhered on the glass slide of the cleaning, and makes
Polypide pad side is contacted with feeler pad side；
(3) adhesive-layer is covered and is arranged in polypide pad；
(4) ultraviolet glue coating is arranged on the junction and feeler pad of feeler pad and polypide pad, and makes purple
The width of outer glue is greater than polypide head width；
(5) water storage The Small Well device is arranged along feeler pad and polypide pad periphery using hot melt adhesive.
Further, the step (3) further includes being adhered to polypide fixing line on the adhesive-layer of polypide pad, and make worm
Body fixing line is located at the cephalothorax merogenesis and chest and abdomen merogenesis of insect.
The use process of living insects feeler directed fixing device in irregular shape:
In use, to insect sample preparation and processing: cutting off the foot and wing of insect, then smear a small amount of sticky ultraviolet glue
The veutro of insect, chest and abdomen is placed in polypide pad again on the junction of polypide pad and feeler pad in feeler pad
On adhesive-layer (double-sided adhesive) on, it is tentatively fixed to complete polypide；Then, it is two-sided to be dipped into polypide pad for veutro part in insect head
In ultraviolet glue on glue；Under Stereo microscope, according to the feeler position that required positioning is observed, such as veutro, back side, inside or outer
Side, it is tentatively fixed to the feeler progress for having determined that position and angle using the high characteristic of the toughness of ultraviolet glue；
By taking feeler veutro fixes (back side exposure) as an example, the foot and wing of insect are first cut off, then smears a small amount of sticky purple
Outer glue on the junction of feeler pad and polypide pad and feeler pad, will cut off the living insects head of foot and wing, chest and
The veutro of abdomen is placed on the adhesive-layer in polypide pad (double-sided adhesive), and it is tentatively fixed to complete polypide；Then, insect head veutro portion
Divide and is dipped into the ultraviolet glue on polypide pad double-sided adhesive；Under Stereo microscope, with thin tweezers by the veutro of feeler or oblique abdomen
Side (back side exposure) is placed in a small amount of ultraviolet glue and is tentatively fixed；Ultraviolet glue, i.e., ultraviolet light irradiation are solidified using ultraviolet lamp
Position 5-8 seconds of ultraviolet glue just solidifies ultraviolet glue, is permanently fixed feeler；In order to further fix polypide, polypide can be fixed
Line is respectively placed between cephalothorax between chest and abdomen, is tensed and is bonded with adhesive-layer (double-sided adhesive), fixed polypide；Finally, in The Small Well
Water is filled in device, and the feeler list sensor in insect back side direction can be seen under high-power microscope amplification and carries out subsequent experimental
Operation.
Working principle:
Using feeler pad as the pedestal of feeler directional at-tachment, facilitates the height difference for coordinating feeler and polypide, meet light transmission
Property；Polypide is placed using polypide pad, fills up the difference in height of body and feeler, polypide is carried out tentatively fixed；Utilize adhesive-layer
It reduces insect body to struggle, facilitates fixation；In order to further fix polypide, preventing struggle, deformation occurs, in polypide pad also
Equipped with polypide fixing line；In feeler pad and ultraviolet glue that feeler pad and polypide pad junction are smeared facilitates fixed touching
Angle and living insects head；Ultraviolet glue is non-toxic and tasteless, flexible, will not generate interference to insect olfaction sensor using ultraviolet glue；
The Small Well device is water storing medium, facilitates and determines sensor type under 1000 times of amplifications by hydroscope and insert electrodes into single sensor
Base portion；
Some insects such as Tephritidae insect, individual is larger, and polypide is not fixed easily；It is relatively thin relative to Drosophilidae feeler cuticula
And form flat the characteristics of being fixed easily, Tephritidae feeler cuticula is thicker (difficult light transmission, the insertion of electrode difficulty), form very irregular,
Multiple types sensor is intensively and cross-distribution, spacing are only 2-3 microns, not only fixes difficulty, but also sensor type identifies, positioning
And operation difficulty, all substantially exceed drosophila.
Beneficial effects of the present invention:
(1) certain insects are directed to, if Tephritidae insect individual is larger, polypide is not fixed easily；Relative to Drosophilidae feeler angle
Matter layer is relatively thin and form flat the characteristics of being fixed easily, and Tephritidae feeler cuticula is thicker (difficult light transmission, the insertion of electrode difficulty), and form is very
Irregularly, multiple types sensor is intensively and cross-distribution, spacing are only 2-3 microns, not only fixes difficulty, but also sensor type is reflected
Not, positioning and operation difficulty, all substantially exceed drosophila；The directed fixing device of living insects feeler in irregular shape of the present invention can
It realizes the quick and synchronous fixation to insect body and form irregular feeler, can especially be wanted according to the position that single sensor is tested
It asks, realizes the directional at-tachment to the special angle of irregular feeler, the single sensor for being satisfied with more highly difficult rank tests micro- behaviour
Make, solve special angle is fixed, feeler cuticula is thicker, light transmittance is low, recording electrode puncture easily lead to target it is mobile and
Only 2-3 microns of single sensor spacing, the problem of exact localization operation hardly possible；
(2) directed fixing device of living insects of the present invention feeler in irregular shape realizes the irregular feeler of Morphology of entomology
Living insects and feeler are carried out while being fixed, will not be subjected to displacement, so that stablizing realizes respectively by the directional at-tachment of all angles
Single sensor tracer signal of angle；
(3) directed fixing device of living insects of the present invention feeler in irregular shape is suitable for all Tephritidae living insects
The fixation of feeler；It is also applied for the fixation of other section (including model insects Drosophilidae) insect feelers；
(4) directed fixing device of living insects of the present invention feeler in irregular shape is easy to operate, high reliablity, experiment effect
Rate is high, low in cost, reusable.
Detailed description of the invention
Fig. 1 is the perspective view of the directed fixing device of living insects feeler in irregular shape；
Fig. 2 is the main view of the directed fixing device of living insects feeler in irregular shape；
1- glass slide, 2- water storage The Small Well device, 3- polypide pad, 4- polypide fixing line, 5- ultraviolet glue, 6- feeler pad in figure
Platform, 7- adhesive-layer；
Fig. 3 is the effect picture of the fixed trypetid feeler of 2 different angle of embodiment, and wherein Fig. 3 A is fixed on the inside of trypetid feeler
(outside exposure) effect picture, Fig. 3 B are (inside exposure) effect picture fixed on the outside of trypetid feeler, and Fig. 3 C is solid for trypetid feeler back side
Fixed (veutro exposure) effect picture, Fig. 3 D are that trypetid feeler veutro fixes (back side exposure) effect picture.
Specific embodiment
The present invention will be further described in the following with reference to the drawings and specific embodiments.But the present invention is not limited to above-mentioned realities
Mode is applied, it within the knowledge of a person skilled in the art, can also be in the premise for not departing from present inventive concept
Under make a variety of changes.
Embodiment 1
As depicted in figs. 1 and 2, a kind of living insects feeler directed fixing device in irregular shape, including glass slide 1, touching
Angle pad 6, polypide pad 3, water storage The Small Well device 2, ultraviolet glue 5, water storage The Small Well device 2 are fixed at the middle part of glass slide 1,
Feeler pad 6 is arranged on glass slide 1 and feeler pad 6 is located in water storage The Small Well device 2, and polypide pad 3 is arranged in glass slide 1
Upper and polypide pad 3 is located in water storage The Small Well device 2, polypide pad 3 be located at 6 side of feeler pad and with 6 side of feeler pad
Contact, 3 upper surface of polypide pad are arranged adhesive-layer 7, apply in feeler pad 6 and the junction of polypide pad 3 and feeler pad 6
It covers and is equipped with ultraviolet glue 5；
The structure of water storage The Small Well device 2 is annulus column or hollow polygon column；
Nearly feeler pad one end of adhesive-layer 7 is less than the 1/2 of insect head length away from the distance of feeler pad 6, adhesive-layer 7
Width and 3 equivalent width of polypide pad；
The height of water storage The Small Well device 2 is 2~3mm；
The width of ultraviolet glue 5 is greater than polypide head width；
It further include polypide fixing line 4, one end of polypide fixing line 4 is fixed on the adhesive-layer 7 of polypide pad 3；Worm
Body fixing line 4 can preferably fix polypide, avoid living insects from struggling and be displaced；
Polypide fixing line 4 is two, is located at the cephalothorax merogenesis and chest and abdomen merogenesis of insect；
The length of feeler pad 6 is 5~20mm, and it is highly 1~2mm that width, which is 3~5mm,；The length of polypide pad 3 is
10~25mm, width are 8~20mm, are highly 0.5~1mm；
The material of water storage The Small Well device is hot melt adhesive；
Feeler pad 6 is glass slide, and polypide pad 3 is coverslip；
Adhesive-layer 7 is double-sided adhesive；
Feeler pad is capable of providing the pedestal of feeler directional at-tachment, facilitates the height difference for coordinating feeler and polypide, meets light transmission
Property；Polypide pad places polypide, fills up the difference in height of body and feeler；Adhesive-layer (double-sided adhesive) in polypide pad can be reduced
Insect body is struggled, the polypide fixing line being arranged in polypide pad, and the fixed line end fixation of polypide is sticked to adhesive-layer (double-sided adhesive)
On, polypide fixing line is pressed respectively against at cephalothorax merogenesis and chest and abdomen merogenesis, further fixes polypide；Feeler pad and polypide pad
Junction and feeler pad on ultraviolet glue can the fixed feeler of slewing and living insects head, feeler knot will not be destroyed
Structure and function；The Small Well device is water storing medium, it can be ensured that antennal sensilla is determined under 1000 times of amplifications by water object lens (× 100)
Type is accurately positioned, and electrode is properly inserted specific sensor with micromanipulative technique；
The production method of living insects feeler directed fixing device in irregular shape, the specific steps are as follows:
(1) glass slide is cut into feeler pad, the bottom surface of feeler pad is adhered in the middle part of clean another glass slide；
(2) coverslip is cut into polypide pad, the bottom surface of polypide pad is adhered on the glass slide of the cleaning, and makes
Polypide pad side is contacted with feeler pad side；
(3) adhesive-layer is covered and is arranged in polypide pad, then polypide fixing line is adhered to the adhesive-layer of polypide pad
On, and it is located at polypide fixing line at the cephalothorax merogenesis and chest and abdomen merogenesis of insect；
(4) ultraviolet glue coating is arranged on the junction and feeler pad of feeler pad and polypide pad, and makes purple
The width of outer glue is greater than polypide head width；
(5) water storage The Small Well device is arranged along feeler pad and polypide pad periphery using hot melt adhesive.
Embodiment 2
Using the directed fixing device of the living insects of embodiment 1 feeler in irregular shape to Tephritidae Bactrocera correcta list
The directional at-tachment of sensor electro physiology experiment living body feeler
Tephritidae Bactrocera correcta adult parted hair, three section of chest and abdomen, are always about 5-8mm；The feeler of Bactrocera correcta is in tool
Aristiform is divided into scapus, pedicel and flagellum；900-1000 μm of feeler overall length, flagellum width~100 μm；In random geometry
And sensor is very intensive, has gathered the sense organ of the multiple functions such as smell, the sense of hearing, temperature and humidity feel and gravity feeling；In order to complete
The working method of feeler is studied in face, understands the different types of single sensor of back side, veutro, inner side and outer side feeler to environmental stimuli
Electrophysiologic response needs to be oriented fixation to it, the specific steps are as follows:
(1) glass slide is cut into the feeler pad of 5mm × 3mm, is adhered to the bottom surface of feeler pad using glue clean
In the middle part of net glass slide；
(2) coverslip is cut into the polypide pad of 10mm × 8mm, the bottom surface of polypide pad is adhered to clean load glass
On piece, and contact polypide pad side with feeler pad side；
(3) adhesive-layer (double-sided adhesive) is covered to be arranged and is adhered to polypide pad in polypide pad, then by polypide fixing line
Adhesive-layer on, and be located at polypide fixing line at the cephalothorax merogenesis and chest and abdomen merogenesis of insect；Using coverslip and carry glass
Difference in height between piece fills up the difference in height of Bactrocera correcta body and feeler；
(4) it is placed under Stereo microscope, it, will before the fixation for carrying out feeler special angle according to the needs of object of experiment
Ultraviolet glue coating is arranged on the junction and feeler pad of feeler pad and polypide pad, and is greater than the width of ultraviolet glue
Polypide head width, width of the ultraviolet glue on the double-sided adhesive of polypide pad is about 2mm, solid convenient for fixed trypetid head and orientation
Determine feeler；
(5) water storage The Small Well device is arranged along feeler pad and polypide pad periphery using hot melt adhesive；In water storage The Small Well device
It is interior to place a small amount of water, the feeler list sensor in insect back side direction can be seen under high-power microscope amplification and carry out subsequent experimental
Operation；
When (outside exposure) fixed on the inside of trypetid feeler, under Stereo microscope, the foot and wing of Bactrocera correcta are cut off；When
When carrying out (outside exposure) fixed on the inside of feeler, a small amount of sticky ultraviolet glue is smeared in the connection of feeler pad and polypide pad
In place and feeler pad, then with thin tweezers insect body two sides are gently clamped, the veutro of living insects head, chest and abdomen is placed in
On double-sided adhesive in polypide pad, it is tentatively fixed to complete polypide；Then, veutro part in head is dipped on polypide pad double-sided adhesive
Ultraviolet glue in；Under Stereo microscope, the inside of feeler or tiltedly inside (outside exposure) are placed in a small amount of purple with thin tweezers
It is tentatively fixed in outer glue；After the completion, ultraviolet glue, the position 5-8 of ultraviolet light irradiation ultraviolet glue are solidified with dedicated ultraviolet lamp
Second, ultraviolet glue is solidified, feeler is permanently fixed；In order to further fix polypide, polypide fixing line can be respectively placed in cephalothorax it
Between between chest and abdomen, tense and and adhered by double sided plaster, fixed polypide；A small amount of water is added in water storage The Small Well device, is shown in height
The lower feeler list sensor that insect back side direction can be seen of micro mirror amplification simultaneously carries out subsequent experimental operation；It is fixed on the inside of trypetid feeler
(outside exposure) effect picture is shown in Fig. 3 A；
When (inside exposure) fixed on the outside of trypetid feeler, under Stereo microscope, the foot and wing of Bactrocera correcta are cut off；When
When carrying out (inside exposure) fixed on the outside of feeler, a small amount of sticky ultraviolet glue is smeared in the connection of feeler pad and polypide pad
In place and feeler pad, then with thin tweezers insect body two sides are gently clamped, the veutro of living insects head, chest and abdomen is placed in
On double-sided adhesive in polypide pad, it is tentatively fixed to complete polypide；Then, it is two-sided to be dipped into polypide pad for veutro part in insect head
In ultraviolet glue on glue；Under Stereo microscope, the outside of feeler or tiltedly outside (inside exposure) are placed on a small quantity with thin tweezers
Ultraviolet glue in tentatively fixed；After the completion, ultraviolet glue, the position of ultraviolet light irradiation ultraviolet glue are solidified with dedicated ultraviolet lamp
5-8 seconds, ultraviolet glue is solidified, is permanently fixed feeler；In order to further fix polypide, polypide fixing line can be respectively placed in cephalothorax
Between between chest and abdomen, tense and and adhered by double sided plaster, fixed polypide；A small amount of water is added in water storage The Small Well device, in high power
The lower feeler list sensor that insect interior direction can be seen of microscope amplification simultaneously carries out subsequent experimental operation；It is solid on the outside of trypetid feeler
Fixed (inside exposure) effect picture is shown in Fig. 3 B；
When trypetid feeler back side fixes (veutro exposure), under Stereo microscope, the foot and wing of Bactrocera correcta are cut off；When
When carrying out feeler back side and fixing (veutro exposure), a small amount of sticky ultraviolet glue is smeared in the connection of feeler pad and polypide pad
In place and feeler pad, then with thin tweezers insect body two sides are gently clamped, the veutro of living insects head, chest and abdomen is placed in
On double-sided adhesive in polypide pad, it is tentatively fixed to complete polypide；Then, it is two-sided to be dipped into polypide pad for veutro part in insect head
In ultraviolet glue on glue；Under Stereo microscope, the back side (veutro exposure) of feeler is placed in a small amount of ultraviolet glue with thin tweezers
Middle progress is tentatively fixed；After the completion, ultraviolet glue is solidified with dedicated ultraviolet lamp, position 5-8 seconds of ultraviolet light irradiation ultraviolet glue, made
Ultraviolet glue solidification, is permanently fixed feeler；In order to further fix polypide, polypide fixing line can be respectively placed between cephalothorax and chest
Between abdomen, tense and and adhered by double sided plaster, fixed polypide；A small amount of water is added in water storage The Small Well device, is put in high-power microscope
The big lower feeler list sensor that insect back side direction can be seen simultaneously carries out subsequent experimental operation；Trypetid feeler back side fixes (veutro
Exposure) effect picture is shown in Fig. 3 C；
When trypetid feeler veutro fixes (back side exposure), under Stereo microscope, the foot and wing of Bactrocera correcta are cut off；When
When carrying out feeler veutro and fixing (back side exposure), a small amount of sticky ultraviolet glue is smeared in the connection of feeler pad and polypide pad
In place and feeler pad, then with thin tweezers insect body two sides are gently clamped, the veutro of living insects head, chest and abdomen is placed in
On double-sided adhesive in polypide pad, it is tentatively fixed to complete polypide；Then it is two-sided to be dipped into polypide pad for veutro part in insect head
In ultraviolet glue on glue；Under Stereo microscope, the veutro (back side exposure) of feeler is placed in a small amount of ultraviolet glue with thin tweezers
Middle progress is tentatively fixed；After the completion, ultraviolet glue is solidified with dedicated ultraviolet lamp, position 5-8 seconds of ultraviolet light irradiation ultraviolet glue, made
Ultraviolet glue solidification, is permanently fixed feeler；In order to further fix polypide, polypide fixing line can be respectively placed between cephalothorax and chest
Between abdomen, tense and and adhered by double sided plaster, fixed polypide；A small amount of water is added in water storage The Small Well device, is put in high-power microscope
The big lower feeler list sensor that insect back side direction can be seen simultaneously carries out subsequent experimental operation；Trypetid feeler veutro fixes (back side
Exposure) effect picture is shown in Fig. 3 D.
The present invention facilitates the height difference for coordinating feeler and polypide using feeler pad as the pedestal of feeler directional at-tachment, full
Sufficient translucency；Polypide pad places polypide, fills up the difference in height of body and feeler；Adhesive-layer (double-sided adhesive) energy in polypide pad
It enough reduces insect body to struggle, the polypide fixing line being arranged in polypide pad, it is (double that polypide fixation line end fixation is sticked to adhesive-layer
Face glue) on, polypide fixing line is pressed respectively against at cephalothorax merogenesis and chest and abdomen merogenesis, further fixes polypide；Feeler pad and worm
The junction of body pad and ultraviolet glue in feeler pad can the fixed feeler of slewing and living insects head, will not destroy
Feeler structure and function；The Small Well device is water storing medium, it can be ensured that touching is determined under 1000 times of amplifications by water object lens (× 100)
Angle sensor type is accurately positioned, and electrode is properly inserted specific sensor with micromanipulative technique.
Living insects feeler directed fixing device in irregular shape of the present invention, which can be realized, does not advise insect body and form pole
The then quick and synchronous fixation of feeler, the position that can be especially tested according to single sensor require, realize to the specific of irregular feeler
The directional at-tachment of angle, be satisfied with more highly difficult rank single sensor experiment micromanipulation, solve special angle fix, feeler
Cuticula is thicker, light transmittance is low, recording electrode puncture easily leads to target movement and single only 2-3 microns of spacing of sensor, accurate fixed
The problem of bit manipulation hardly possible.
Claims (9)
1. a kind of living insects feeler directed fixing device in irregular shape and preparation method thereof, it is characterised in that: including carrying glass
Piece (1), feeler pad (6), polypide pad (3), water storage The Small Well device (2), ultraviolet glue (5), water storage The Small Well device (2) fixation are set
The middle part in glass slide (1) is set, feeler pad (6) is arranged on glass slide (1) and feeler pad (6) is located at water storage The Small Well device
(2) in, polypide pad (3) is arranged on glass slide (1) and polypide pad (3) is located in water storage The Small Well device (2), polypide pad
(3) it is located at feeler pad (6) side and is contacted with feeler pad (6) side, adhesive-layer (7) are arranged in polypide pad (3) upper surface,
Coating is provided with ultraviolet glue (5) on the junction and feeler pad (6) of feeler pad (6) and polypide pad (3).
2. living insects feeler directed fixing device in irregular shape according to claim 1, it is characterised in that: water storage The Small Well
The structure of device (2) is annulus column or hollow polygon column.
3. living insects feeler directed fixing device in irregular shape according to claim 1, it is characterised in that: adhesive-layer
(7) nearly 1/2 of distance of the feeler pad one end away from feeler pad (6) less than insect head length, the width of adhesive-layer (7) and worm
Body pad (3) equivalent width.
4. living insects feeler directed fixing device in irregular shape according to claim 1, it is characterised in that: water storage The Small Well
The height of device (2) is 2~3mm.
5. living insects feeler directed fixing device in irregular shape according to claim 1, it is characterised in that: ultraviolet glue
(5) width is greater than polypide head width.
6. living insects feeler directed fixing device in irregular shape according to claim 1, it is characterised in that: further include two
Rootworm body fixing line (4), one end of polypide fixing line (4) are fixed on the adhesive-layer (7) of polypide pad (3).
7. living insects feeler directed fixing device in irregular shape according to claim 1, it is characterised in that: feeler pad
(6) length is 5~20mm, and it is highly 1~2mm that width, which is 3~5mm,；The length of polypide pad (3) is 10~25mm, width
It is highly 0.5~1mm for 8~20mm.
8. the production method of living insects feeler directed fixing device in irregular shape, which is characterized in that specific step is as follows:
(1) glass slide is cut into feeler pad, the bottom surface of feeler pad is adhered in the middle part of the glass slide of another cleaning；
(2) coverslip is cut into polypide pad, the bottom surface of polypide pad is adhered on the glass slide of the cleaning, and make polypide
Pad side is contacted with feeler pad side；
(3) adhesive-layer is covered and is arranged in polypide pad；
(4) ultraviolet glue coating is arranged on the junction and feeler pad of feeler pad and polypide pad, and makes ultraviolet glue
Width be greater than polypide head width；
(5) water storage The Small Well device is arranged along feeler pad and polypide pad periphery using hot melt adhesive.
9. the production method of the feeler directed fixing device in irregular shape of living insects described in claim 8, it is characterised in that: step
It suddenly further include being adhered to polypide fixing line on the adhesive-layer of polypide pad, and polypide fixing line is made to be located at insect in (3)
Cephalothorax merogenesis and chest and abdomen merogenesis at.
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Cited By (1)
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| CN111458334A (en) * | 2020-04-16 | 2020-07-28 | 云南大学 | Visualization method for lymphatic vessels in light-color antennae of insects |
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| CN110286016B (en) | 2023-08-04 |
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