CN208705561U - A kind of mini microscope for Analysis of epidemic disease - Google Patents
A kind of mini microscope for Analysis of epidemic disease Download PDFInfo
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- CN208705561U CN208705561U CN201821517643.3U CN201821517643U CN208705561U CN 208705561 U CN208705561 U CN 208705561U CN 201821517643 U CN201821517643 U CN 201821517643U CN 208705561 U CN208705561 U CN 208705561U
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- lens
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- probe
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Abstract
The utility model discloses a kind of mini microscopes for Analysis of epidemic disease, including probe, laser emitting module, signal processing module, image display and main control module；Wherein, probe is that miniature mating plate micro-imaging is popped one's head in, it is installed on object under test, it is excited by probe transmitting mating plate measuring targets, and fluorescence signal is collected on the direction perpendicular to mating plate and is imaged, the fluoroscopic image of object under test is obtained, probe includes illumination path unit and imaging optical path unit.The utility model is excited using mating plate scanning measuring targets, improve image taking speed and signal-to-noise ratio, reduce phototoxicity, and the illumination path unit of the utility model changes mating plate direction using back-shaped guide structure, laser is uniform via the mating plate visual field that back-shaped guide structure is formed, the visual effect in operating process is improved, and enhances the accuracy of microexamination.
Description
Technical field
The utility model relates to a kind of mini microscopes for Analysis of epidemic disease, belong to pathological analysis auxiliary tool technology
Field.
Background technique
Microscope is indispensable tool during cytomorphology is examined, and mating plate microscope is using micron-sized smooth thin slice
Sample is excited, rapid fluorescence signal detection is carried out to sample in the direction of vertical mating plate.Mating plate microscope is imaged for sample,
The speed for obtaining image than traditional spot scan with light flat scanning object faster, there is better signal-to-noise ratio and lower light poison
Property, there is preferable optics to select layer ability, background signal can be reduced, obtain the image of higher contrast.But current many light
The animal that piece microscope imaging device is only applicable to anesthesia or head is fixed, and some imaging devices are than cumbersome, it is unsuitable
It is mounted on animal head.Chinese invention patent CN107966799A discloses a kind of miniature mating plate microscope of wear-type, can be with
Preferably it is suitable for loose-jointed animal brain imaging, which is installed on probe on the head of object under test
Portion, exciting light is excited by moulding of popping one's head at detecting object is treated after mating plate, and is collected on the direction perpendicular to mating plate
Fluorescence signal is imaged, and the neural fluoroscopic image of object under test is obtained.But the structure existing defects, illumination unevenness are leaned on
The position of nearly prism and illuminating lens is obviously brighter, and the position of principle prism and illuminating lens then illuminates deficiency in visual field, this
Cause entire field luminance uneven, still receive in the case where visually observing, but carries out figure using image-forming component such as CCD
To occur close to the position of prism apparent over-exposed in the case where as acquisition, in visual field, and the position far from prism will be by
There is more noise in under-exposure, influences the accuracy of microexamination, and influence the visual experience in operating process.
Summary of the invention
The purpose of this utility model is to provide a kind of mini microscopes for Analysis of epidemic disease, using mating plate scanning pair
Object under test is excited, and changes mating plate direction using back-shaped guide structure in optic path process, the mating plate of formation
Visual field is uniform, improves the visual effect in operating process and the accuracy of microexamination.
In order to achieve the above objectives, the technical solution adopted in the utility model is as follows:
A kind of mini microscope for Analysis of epidemic disease, including probe, laser emitting module, signal processing module, figure
As display module and main control module；The laser emitting module is connected by optical fiber with probe, the laser emitting module, letter
Number processing module and image display are connected with main control module, and the signal processing module is also shown with probe and image
Module connection；
The laser emitting module uses laser；The signal processing module is believed using the AP0100CS image of Aptina
Number processor；Described image display module uses display screen；The main control module uses computer；
The probe is installed on object under test, and the probe includes illumination path unit and imaging optical path unit, the photograph
Bright optical path unit is made of collimation lens, reflecting mirror, cylindrical lens, prism, back-shaped guide structure and illuminating lens, it is described at
As optical path unit is made of object lens, imaging len, optical filter and Electro-Optical Sensor Set；
The collimation lens volume focus is located at fibre optical transmission port；
The normal of the reflecting surface of the reflecting mirror and the laser after collimation lens are at 45 °；
The central axis of the cylindrical lens is parallel with the emergent light of the reflecting mirror；
The prism uses the prism of equilateral right angled triangle, and the right angle side length of the prism is matched with illuminating lens, instead
Penetrating face is the face where hypotenuse；
The fixed back-shaped guide structure of the other end of the illuminating lens；One end face middle part of the back-shaped guide structure
Position is illumination interface, is adhesively fixed with illuminating lens by optical cement；The underface of the illumination interface, back-shaped light guide knot
Setting reflection triangular prism inside structure；The quadrangle of the back-shaped guide structure is set as unfilled corner structure；The back-shaped guide structure is set out
Smooth surface；The back-shaped guide structure plates reflectance coating except all faces of light-emitting surface；
The object lens are arranged close to illuminating lens, and are parallel to each other with illuminating lens central axis；The back-shaped light guide knot
The hollow part of structure is located at the underface of object lens；
The object lens are placed perpendicular to object under test plane；Imaging is set gradually on the direction consistent with objective lens pupil
Lens, optical filter and Electro-Optical Sensor Set；
The pupil of the imaging len is aligned with object lens.
Collimation lens above-mentioned uses plano-convex lens；
The reflecting mirror uses equilateral right angled triangle reflecting mirror；
The cylindrical lens uses piano convex cylindrical lens；
The illuminating lens uses self-focusing lens；
The object lens have the self-focusing lens of color correction eyeglass using combination；
The imaging len uses achromatic lens；
The Electro-Optical Sensor Set uses COMS imaging device.
Reflection triangular prism above-mentioned is embedded in inside back-shaped guide structure entity, and is plated and reflected on the surface of the reflection triangular prism
Film.
Reflection triangular prism above-mentioned uses the form that a triangular prism notch is opened up in back-shaped guide structure, the notch
Outer wall plates reflectance coating.
Reflectance coating above-mentioned is metallic film.
The medial surface of back-shaped guide structure above-mentioned is light-emitting surface.
The downside of back-shaped guide structure above-mentioned is light-emitting surface.
The self-focusing lens length that illuminating lens above-mentioned uses is 0.24 pitch, diameter 1mm, numerical aperture 0.2.
Length is 6.2mm, diameter 1.4mm, numerical aperture 0.8 after object lens combination above-mentioned.
The utility model it is achieved the utility model has the advantages that
The utility model in such a way that mating plate scans the Mapping slow instead of image taking speed, measuring targets into
When row excitation, there is mating plate good optics to select layer ability and biggish excitation area, improve image taking speed and signal-to-noise ratio, drop
Low phototoxicity is suitable for prolonged living imaging, obtains higher contrast image.The utility model uses back-shaped light guide knot
Structure changes mating plate direction, and the mating plate visual field that laser is formed via back-shaped guide structure is uniform, improves in operating process
Visual effect, and enhance the accuracy of microexamination.
Detailed description of the invention
Fig. 1 is the sonde configuration schematic diagram of the mini microscope for Analysis of epidemic disease of the utility model；
Fig. 2 is the back-shaped guide structure schematic diagram in the probe of the utility model；
Fig. 3 is back-shaped guide structure, the installation relation schematic diagram of illuminating lens and object lens；
Fig. 4 is the light path schematic diagram in back-shaped guide structure；
Fig. 5 is each module connection diagram of the utility model.
Specific embodiment
It is right with reference to the accompanying drawings and examples in order to make those skilled in the art that the utility model may be better understood
Technical solutions of the utility model further illustrate.
A kind of mini microscope for Analysis of epidemic disease provided by the utility model, including probe, laser emitting module,
Signal processing module, image display and main control module.
Specifically, laser emitting module is connected by optical fiber 1 with probe referring to Fig. 1 and Fig. 5, laser emitting module, letter
Number processing module and image display are connected with main control module, signal processing module also with probe and image display
Connection.
Laser emitting module uses laser, for generating and exporting laser, and is transmitted through the fiber to probe.
Fluorescence electric signal caused by signal processing module receiving transducer carries out being delivered to image respectively after simple process aobvious
Show module and main control module.The utility model is using the AP0100CS image-signal processor of Aptina as signal processing mould
Block is, it can be achieved that simple signal processing.
Image display uses display screen, to by signal processing module, treated that fluorescence signal is shown.
Main control module shows fluorescence signal for controlling laser emitting module transmitting laser, control image display
Show, processing analysis can also be carried out to the signal and display result of collection.Main control module controls laser emitting module and emits laser,
The laser emitted is transmitted through the fiber to probe, after laser passes through the illumination path unit moulding popped one's head in into mating plate, with mating plate
Object under test is sent to be excited, the imaged optical path unit of the fluorescence signal that object under test is generated for excitation collects imaging,
And it is delivered to signal processing module, signal processing module will treated that signal is delivered to image display shows, and
It is delivered to main control module and carries out signal analysis.
Main control module is realized using computer in the utility model, analysis treatment process to signal and to laser
It with the control of display screen, can be realized by conventional setting and software well known in the art, and it is to data
Treatment process is analyzed not within the protection category of the utility model.
Probe is installed on object under test, is excited by probe transmitting mating plate measuring targets, and perpendicular to mating plate
Direction on collect fluorescence signal and be imaged, obtain the fluoroscopic image of object under test, probe be set as miniature mating plate it is micro- at
As probe, structural schematic diagram is as shown in Figure 1.
Probe includes illumination path unit and imaging optical path unit, wherein illumination path unit is by collimation lens 2, reflection
Mirror 3, cylindrical lens 4, prism 5, back-shaped guide structure 11 and illuminating lens 6 are constituted, and imaging optical path unit is by object lens 7, imaging
Lens 8, optical filter 9 and Electro-Optical Sensor Set 10 are constituted.
Specifically, the laser that collimation lens 2 is used to generate laser emitting module collimates, collimated in the utility model
Lens 2 use plano-convex lens, and collimation lens focus is located at 1 emission port of optical fiber.
Reflecting mirror 3 is used to reflect by the laser of collimation lens 2, makes laser propagation to cylindrical lens 4, this is practical
Novel middle reflecting mirror use equilateral right angled triangle reflecting mirror, the normal of reflecting surface with after collimation lens 2 laser at
45°。
Cylindrical lens 4 forms mating plate, this is practical new for that will focus slabbing by the laser that reflecting mirror 3 reflects back
Type central column face lens 4 use piano convex cylindrical lens, and the laser through reflecting mirror 3 is injected along with 4 central axis parallel direction of cylindrical lens
The convex surface of cylindrical lens 4.
Prism 5 makes laser reflection enter illuminating lens for changing mating plate direction.Prism is using equilateral straight in the utility model
The prism of angle triangle, and right angle side length is matched with illuminating lens, and reflecting surface is the face where hypotenuse.
Illuminating lens 6 is for being imaged mating plate.Illuminating lens uses self-focusing lens in the utility model.This is practical
The self-focusing lens length that novel middle illuminating lens uses is 0.24 pitch, diameter 1mm, numerical aperture 0.2.
Back-shaped guide structure 11 is used to mating plate projecting object under test.Referring to fig. 2, back-shaped guide structure 11 is chosen
Position is adhesively fixed with illuminating lens 6 by optical cement, as illumination interface 13 in the illumination interface among one end face
13 underface, back-shaped guide structure inside setting reflection triangular prism 12, the reflection triangular prism can be embedded in back-shaped guide structure
Inside entity, a triangular prism notch can also be opened up in the back-shaped guide structure.If it is in back-shaped guide structure entity
It is internally embedded reflection triangular prism, then reflectance coating is plated on the surface of the reflection triangular prism, back-shaped for incident laser reflection to be entered this
In structure；If it is a triangular prism notch is opened up in back-shaped guide structure, then reflectance coating is plated in notch outer wall, it equally can be with
Incident laser reflection is entered in the back-shaped structure.Referring to fig. 2, which forms two reflectings surface, will be by illumination
The laser reflection of interface incidence is to both direction.Further, the quadrangle of back-shaped guide structure 5 is set as unfilled corner structure, outside
Reflectance coating is plated in side, in this way, the light of the reflective surface through reflecting triangular prism is in four corners secondary reflection again, to keep light equal
It is even to be distributed in reversion guide structure, form uniform mating plate.The medial surface that the back-shaped guide structure is arranged is light-emitting surface, and
Except its lap of light-emitting surface plates reflectance coating, the visual field of uniform illumination is formed in back-shaped guide structure hollow part at this time, it is incident
On object under test, excitation object under test emits fluorescence.Also the downside that the back-shaped guide structure can be set is light-emitting surface, this
When medial surface plate reflectance coating, reflectance coating is not plated in downside.
Referring to Fig. 1 and Fig. 3, object lens 7 are arranged close to illuminating lens 6 in the utility model, and illuminating lens 6 and object lens 7
Central axis is parallel to each other, and back-shaped guide structure 11 is adhesively fixed by illuminating interface 13 with illuminating lens 6, hollow part
It is located just at the underface of object lens 7, in this way, forming uniform visual field in hollow part by the light-emitting surface of back-shaped guide structure, is shone
It penetrates on testee, excitation testee emits fluorescence, collected by object lens.
Back-shaped guide structure as shown in Figure 4, arrow is the transmission direction of light in figure, and two of triangular prism are reflected in figure
Faceted pebble is reflecting surface 14, and incident laser advances through two reflections of reflecting surface 14 along the both direction of back-shaped guide structure, four
Continuing to be reflected at angle, finally forms uniform mating plate in entire back-shaped structure, in the figure, medial surface is light-emitting surface, at this point,
Uniform visual field is formed in the hollow part of back-shaped guide structure.
Reflectance coating is metallic film in the utility model.
Object lens 7 are imaged for collecting the fluorescence emitted after object under test is excited, and object lens use in the utility model
Combination has the self-focusing lens of color correction eyeglass, places perpendicular to object under test plane.In the side consistent with objective lens pupil
Imaging len 8, optical filter 9 and Electro-Optical Sensor Set 10 are set gradually upwards.Length is after object lens combination in the utility model
6.2mm, diameter 1.4mm, numerical aperture 0.8.
Imaging len 8 is used to be corrected color difference and further enlarging objective imaging, is imaged in the utility model
Lens use achromatic lens, its pupil is arranged and is aligned with object lens.
Optical filter 9 is for filtering out interference light, and by the fluorescence of collection, optical filter 9 is set to imaging len 8 and photodetection
Between device 10.
Electro-Optical Sensor Set 10 is used to the optical signal being collected into being converted to electric signal, and photodetection fills in the utility model
It sets using COMS imaging device.
The working principle of the utility model are as follows: main control module controls laser emitting module and emits laser, the laser emitted
It is transmitted to probe by optical fiber 1, collimated lens 2 are reflected after collimating by reflecting mirror 3, form mating plate, mating plate by cylindrical lens 4
It is reflected through illuminating lens 6 through prism 5, then shines object under test through the reflection of back-shaped guide structure 5, mating plate excites object under test hair
Fluorescence out；Object lens 7 are set on the direction perpendicular to mating plate, the picture collecting the fluorescence of object under test transmitting and amplifying at one,
Then, imaging len 8 carries out chromatic aberration correction to imaging and will interfere as further being amplified, then through optical filter 9
Light is imaged in Electro-Optical Sensor Set 10 after filtering out.Imaging is delivered to signal processing module, and signal processing module carries out letter
Signal is delivered to image display after single processing to show, and is delivered to main control module and carries out signal analysis.
The mini microscope of the utility model can be used for detecting human skin or tissue or organ, and object under test can be
The form of slice or slide.
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art
For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and
Retouching also should be considered as the protection scope of the utility model.
Claims (9)
1. a kind of mini microscope for Analysis of epidemic disease, which is characterized in that at probe, laser emitting module, signal
Manage module, image display and main control module；The laser emitting module is connected by optical fiber with probe, the laser hair
Module, signal processing module and image display is penetrated to be connected with main control module, the signal processing module also with probe
It is connected with image display；
The laser emitting module uses laser；The signal processing module uses at the AP0100CS picture signal of Aptina
Manage device；Described image display module uses display screen；The main control module uses computer；
The probe is installed on object under test, and the probe includes illumination path unit and imaging optical path unit, the illumination light
Road unit is made of collimation lens, reflecting mirror, cylindrical lens, prism, back-shaped guide structure and illuminating lens, the imaging
Road unit is made of object lens, imaging len, optical filter and Electro-Optical Sensor Set；
The collimation lens volume focus is located at fibre optical transmission port；
The normal of the reflecting surface of the reflecting mirror and the laser after collimation lens are at 45 °；
The central axis of the cylindrical lens is parallel with the emergent light of the reflecting mirror；
The prism uses the prism of equilateral right angled triangle, and the right angle side length of the prism is matched with illuminating lens, reflecting surface
For the face where hypotenuse；
The fixed back-shaped guide structure of the other end of the illuminating lens；Position is among one end face of the back-shaped guide structure
Interface is illuminated, is adhesively fixed with illuminating lens by optical cement；The underface of the illumination interface, in back-shaped guide structure
Portion's setting reflection triangular prism；The quadrangle of the back-shaped guide structure is set as unfilled corner structure；The back-shaped guide structure sets out smooth surface；
The back-shaped guide structure plates reflectance coating except all faces of light-emitting surface；
The object lens are arranged close to illuminating lens, and are parallel to each other with illuminating lens central axis；The back-shaped guide structure
Hollow part is located at the underface of object lens；
The object lens are placed perpendicular to object under test plane；It is saturating that imaging is set gradually on the direction consistent with objective lens pupil
Mirror, optical filter and Electro-Optical Sensor Set；
The pupil of the imaging len is aligned with object lens.
2. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that
The collimation lens uses plano-convex lens；
The reflecting mirror uses equilateral right angled triangle reflecting mirror；
The cylindrical lens uses piano convex cylindrical lens；
The illuminating lens uses self-focusing lens；
The object lens have the self-focusing lens of color correction eyeglass using combination；
The imaging len uses achromatic lens；
The Electro-Optical Sensor Set uses COMS imaging device.
3. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that the reflection three
Prism is embedded in inside back-shaped guide structure entity, and plates reflectance coating on the surface of the reflection triangular prism.
4. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that the reflection three
Prism uses the form that a triangular prism notch is opened up in back-shaped guide structure, and the notch outer wall plates reflectance coating.
5. a kind of mini microscope for Analysis of epidemic disease according to claim 3 or 4, which is characterized in that described anti-
Penetrating film is metallic film.
6. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that the back-shaped light
The medial surface of guide structure is light-emitting surface.
7. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that the back-shaped light
The downside of guide structure is light-emitting surface.
8. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that the illumination is saturating
The self-focusing lens length that mirror uses is 0.24 pitch, diameter 1mm, numerical aperture 0.2.
9. a kind of mini microscope for Analysis of epidemic disease according to claim 1, which is characterized in that the objective lens
Length is 6.2mm, diameter 1.4mm, numerical aperture 0.8 after conjunction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821517643.3U CN208705561U (en) | 2018-09-17 | 2018-09-17 | A kind of mini microscope for Analysis of epidemic disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821517643.3U CN208705561U (en) | 2018-09-17 | 2018-09-17 | A kind of mini microscope for Analysis of epidemic disease |
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| Publication Number | Publication Date |
|---|---|
| CN208705561U true CN208705561U (en) | 2019-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821517643.3U Expired - Fee Related CN208705561U (en) | 2018-09-17 | 2018-09-17 | A kind of mini microscope for Analysis of epidemic disease |
Country Status (1)
| Country | Link |
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| CN (1) | CN208705561U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392729A (en) * | 2023-05-29 | 2023-07-07 | 广州市五合医疗器械有限公司 | Laser therapeutic instrument |
-
2018
- 2018-09-17 CN CN201821517643.3U patent/CN208705561U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392729A (en) * | 2023-05-29 | 2023-07-07 | 广州市五合医疗器械有限公司 | Laser therapeutic instrument |
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