CN208705230U - A kind of double channel optical fiber surface plasmon resonance biosensor - Google Patents
A kind of double channel optical fiber surface plasmon resonance biosensor Download PDFInfo
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
- G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Abstract
The utility model relates to a kind of double channel optical fiber spr sensors.The binary channels spr sensor includes reference channel and sensing passage as blank control reference channel and detects the wavelength amount of movement as caused by non-specific adsorption；Sensing passage improves detection sensitivity using the coupling effect enhancing local electric field intensity between golden film and gold nanoparticle, and the fixed efficiency of antibody is improved using graphene oxide film.Two channels use different metal films respectively in double channel optical fiber spr sensor, to generate two resonance paddy, realize binary channels sensing, have measurement error and temperature-resistant unique advantage caused by eliminating non-specific adsorption；Two SPR resonance paddy of binary channels are separated from each other simultaneously, it is ensured that they will not mutually have an adverse effect in the detection process, it is made to be more advantageous to bio-sensing.The utility model solve the problems, such as transducer sensitivity in the prior art it is low, vulnerable to liquid refractivity variation and temperature fluctuations affect.
Description
Technical field
The utility model relates to biosensor technology fields, and in particular to surface plasmon resonance biosensor, more particularly to it is a kind of double
Channel fiber surface plasmon resonance biosensor.
Background technique
Surface Plasmon Resonance (SPR), Chinese are surface plasma body resonant vibration, it is a kind of normal
The optical phenomena seen, when it refers to that the incident light of a certain specific wavelength in optical waveguide is irradiated to metal (such as gold or silver) film, light
The plasma wave that wave will be generated with metal surface resonates, referred to as surface plasma body resonant vibration (SPR) effect.SPR
Effect can make the energy of reflected light fall sharply, so that resonance trough is formed, meanwhile, SPR is very sensitive to extraneous refractive index, when
When surveyed solution concentration change, i.e. extraneous solution refraction index changing, the resonance trough of SPR will be moved, and therefore, this is practical
The novel offset by detection SPR resonance trough realizes bio-sensing.
Surface plasmon resonance biosensor is caused greatly in biological and chemical detection due to its highly sensitive and biocompatibility
Concern.According to the interaction between surface plasma wave and surrounding biologic molecule, lead to resonance angle or resonant wavelength
Offset, so that it may realize the detection of external biomolecule.Compared with traditional SPR bioanalytical sensing platform based on prism, optical fiber
Biosensor has production simply, at low cost, the characteristics such as miniaturized structure and electromagnetism interference.However, traditional SPR biology
Sensor lacks enough sensitivity and carrys out the lower biomolecule of detection molecules quality, such as the biological sample of DNA and low concentration.
In addition, only one sense channel of most of optical fiber biosensors, can only detect a kind of analyte, and in the detection process, inspection
The accuracy for surveying result would generally be fluctuated by the non-specific adsorption or ambient temperature of biological sample to be influenced.Therefore, it is necessary to
Further increase the detection sensitivity and accuracy of sensor.
Utility model content
Sensitivity and accuracy in order to solve the problems, such as existing fiber surface plasmon resonance biosensor is lower, and the utility model mentions
A kind of double channel optical fiber spr sensor is gone out.The binary channels spr sensor includes reference channel and sensing passage, as blank
The wavelength amount of movement as caused by non-specific adsorption is detected in control reference channel；Sensing passage utilizes golden film and gold nanoparticle
Between coupling effect enhancing local electric field intensity improve the detection sensitivity of sensor, in the photonic crystal light for being coated with golden film
Graphene oxide film is fixed by Covalent bonding together method in the surface of fiber sensor, is improved using graphene oxide film
The fixed efficiency of antibody, antibody and causes resonant wavelength to drift about by the specific binding between antigen that gold nanoparticle is fixed,
The measurement of antigen high sensitivity is realized according to the amount of movement of the resonant wavelength shown on spectrometer；Double channel optical fiber SPR sensorgram
Two channels use different metal films respectively in device, to generate two resonance paddy, realize binary channels sensing.
To achieve the above object, the utility model uses following technical scheme:
A kind of double channel optical fiber surface plasmon resonance biosensor, including reference channel and sensing passage, reference channel and sense channel
It is connected by multimode fibre, in which:
Reference channel includes the first photonic crystal fiber, and welding multimode fibre is distinguished at the first photonic crystal fiber both ends, the
One photonic crystal fiber electroplate film；Reference channel detects the wavelength as caused by non-specific adsorption and moves as blank control
Momentum；
Sensing passage includes the second photonic crystal fiber, and welding multimode fibre is distinguished at the both ends of the second photonic crystal fiber,
Graphene oxide film is fixed on second photonic crystal fiber surface gold-plating film, golden film surface, and solidifying on graphene oxide film has
There is the antibody film layer of selective absorbing to biomolecule or chemical component, the antigen corresponding to antibody is consolidated by gold nanoparticle
It is fixed, antibody and antigen binding in detection process, so that the gold nanoparticle that surface is fixed with antigen is fixed on antibody film, gold
Coupling effect is generated between film and gold nanoparticle.
Further, the second photonic crystal fiber of the first photonic crystal fiber of the reference channel and sensing passage
Length be 0.5~2cm.
Further, the first photon crystal surface of the reference channel plates silverskin, and silver film thickness is 40~60nm.
Further, the second photonic crystal fiber surface gold-plating film of the sensing passage, golden film with a thickness of 40~
60nm。
Further, the fixed graphene oxide film in the golden film surface of the sensing passage, golden film surface pass through covalent
Conjunction method is bonded to fix graphene oxide film, the fixed efficiency of antibody is improved using graphene oxide film.
Further, the fixed graphene oxide film in the golden film surface of the sensing passage, graphene oxide film
With a thickness of 0.2~0.6nm.
Further, the diameter of the gold nanoparticle of the sensing passage is 10~50nm.
The principles of the present invention are as follows:
The utility model mainly improves the sensitivity and accuracy of optical fiber SPR sensor detection using binary channels, wherein
For one section of photonic crystal fiber as sensing passage, surface successively plates golden film and graphene oxide film, is used for sessile antibody, resists
The non-specific adsorption of specific binding and antigen between former and antibody can all cause the wavelength of sensing passage mobile, in order to arrange
Except wavelength amount of movement caused by the non-specific adsorption of antigen in sensing passage, the photonic crystal fiber of another section of plating silverskin is introduced
It is for detecting wavelength amount of movement caused by the non-specific adsorption of antigen, the wavelength of sensing passage is mobile as reference channel
The wavelength amount of movement that amount subtracts reference channel has just obtained wavelength amount of movement caused by specifically binding between antigen and antibody, by
This accuracy to improve detection.
The double channel optical fiber SPR sensorgram system formed by above-mentioned double channel optical fiber surface plasmon resonance biosensor, including with multimode light
Fibre is double channel optical fiber spr sensor, wideband light source and the spectrometer of optical path, and double channel optical fiber spr sensor input terminal passes through
For the optical transport that multimode fibre optical path issues wideband light source to double channel optical fiber spr sensor, double channel optical fiber spr sensor is defeated
Outlet connects spectrometer, and spectrometer is connected to computer by data-interface, and double channel optical fiber surface plasmon resonance biosensor is placed in be checked
It surveys in the solution of biomolecule.
Further, the double channel optical fiber SPR sensorgram system is placed in closed tubulose xylometer to be checked, to be detected
Container both ends are provided with injection port and outlet.
Further, tubulose xylometer to be checked has fixed bracket, and fixed bracket supports tubulose appearance to be detected
Device is placed in fixed position.
Sensing passage outer surface utilizes oxygen as bio-sensing channel from inside and outside gold-plated film and graphene oxide film respectively
Graphite alkene film improves the fixed efficiency of antibody, specific binding between antibody and the antigen fixed by gold nanoparticle
Cause resonant wavelength to drift about, the measurement of antigen high sensitivity is realized according to the amount of movement of the resonant wavelength shown on spectrometer.
Silverskin is plated in the outer surface of reference channel, as blank control, detects wavelength amount of movement caused by non-specific adsorption, Jin Erti
The accuracy of high detection.
The preparation method of above-mentioned double channel optical fiber spr sensor, steps are as follows:
(1) double channel optical fiber sensor is prepared
Welding multimode fibre is distinguished by heat sealing machine in the both ends of first photonic crystal fiber and the second photonic crystal fiber,
That is it is connected between two sections of photonic crystal fibers also by the multimode fibre of welding, the first photonic crystal fiber and the second photonic crystal
The length of optical fiber is all 0.5~2cm, and strength of discharge when welding is 50~100mW, and discharge current is 3000~5000mA, is melted
Jointing temp is 2000~3000 DEG C；
(2) reference channel is prepared
By silver mirror reaction to the first photonic crystal fiber electroplate film, silver film thickness is 40~60nm；
(3) sensing passage is prepared
I gold-plated film
Second photonic crystal fiber is put into vacuum ionic beam sputtering instrument, to the second photonic crystal fiber surface gold-plating
Film, the electric current of vacuum ionic beam sputtering instrument are 5~7mA, and the time is 2~4 minutes, and golden film is with a thickness of 40~60nm；
II fixes graphene oxide film
Second photonic crystal fiber of gold-plated film is immersed 6 in the 4- aminothiophenol ethanol solution of 0.5~2mmol/L~
24 hours, to carry out golden film surface amination, 4- aminothiophenol molecule can be connect by Au-S covalent bond with golden film, and will
Amido (- NH2) is left outside, with further in conjunction with the epoxy group of graphene oxide；After distilled water flushing, the second photon is brilliant
Body optical fiber immerses in the graphene oxide water solution of 0.05~1mg/mL, and putting the graphene oxide water solution into temperature is 30
In~60 DEG C of insulating box, 20~after sixty minutes, graphene oxide dispersion evaporation is realized by physical evaporation method in gold
Film surface fix graphene oxide film, graphene oxide film with a thickness of 0.2~0.6nm；
III sessile antibody
Second photonic crystal fiber is immersed in 1- ethyl-(the 3- dimethyl that 0.5~1mL concentration is 0.1~0.4mol/L
Aminopropyl) phosphinylidyne diimmonium salt hydrochlorate and 0.5~1mL concentration be 0.1~0.4mol/L n-hydroxysuccinimide it is mixed
It closes in solution, 15~after sixty minutes, distilled water flushing is clean；It is then immersed in antibody-solutions to be fixed and is resisted
Body is fixed；
IV gold nanoparticle immobilized antigen
Antigen to be detected is added to the golden nanometer particle dispersion liquid that concentration is 500~1000ppm, diameter is 10~50nm
In, antigen is fixed on to the surface of gold nanoparticle by electrostatic self-assembled；
(4) antigen is detected
At 25~37 DEG C of temperature, the people fixed by gold nanoparticle is added to double channel optical fiber spr sensor, ball is immunized
Protein solution causes resonant wavelength to drift about according to antibody and by the specific binding between antigen that gold nanoparticle is fixed, real
The detection of existing antigen, while the specific adsorption between practical Ag-Ab is obtained according to the wavelength amount of movement of reference channel
Caused wavelength amount of movement.
The beneficial effect of the utility model compared with prior art is:
1, the utility model proposes double channel optical fiber spr sensor pass through setting reference channel and sensing passage bilateral
Road, the high sensitivity having eliminate measurement error and temperature-resistant unique advantage caused by non-specific adsorption；
2, twin-channel two SPR resonance paddy is separated from each other, and will not mutually be generated not with ensuring them in the detection process
Benefit influences, it is made to be more advantageous to bio-sensing；
To sum up, the utility model solve transducer sensitivity in the prior art it is low, vulnerable to liquid refractivity variation and
The problem of temperature fluctuations affect, is very suitable for being widely popularized in fields such as bio-sensings.
Detailed description of the invention
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The some embodiments recorded in application, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings.
Fig. 1 is in the utility model embodiment 1 based on double channel optical fiber surface plasmon resonance biosensor structural schematic diagram；
Fig. 2 is the double channel optical fiber SPR biological sensing system structural schematic diagram in the utility model embodiment 1；
Fig. 3 is spectrogram of the double channel optical fiber spr sensor under different refractivity in the utility model embodiment 1；
Fig. 4 is that refractive index of the double channel optical fiber spr sensor under different refractivity in the utility model embodiment 1 is quasi-
Close curve graph；
Fig. 5 is people's immune globulin of the double channel optical fiber spr sensor detection various concentration in the utility model embodiment 1
White corresponding wavelength amount of movement；
In figure: 1, first photonic crystal fiber, the 2, second photonic crystal fiber, 3, multimode fibre, 4, silverskin, 5, golden film,
6, graphene oxide film, 7, antibody-goat anti-human immunoglobulin, 8, antigen-human immunoglobulin(HIg), 9, gold nanoparticle, 1 ',
Reference channel, 2 ', sensing passage, A, high sensitivity optical fiber surface plasmon resonance biosensor, B, wideband light source, C, spectrometer, D, multimode light
Fine optical path, E, injection port, F, outlet, G, computer, H, glass tube, I, bracket.
Specific embodiment
It should be noted that in the absence of conflict, the feature in the embodiments of the present invention and embodiment can
To be combined with each other.The utility model will be described in detail below with reference to the accompanying drawings and embodiments.
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is only the utility model a part of the embodiment, instead of all the embodiments.Below at least one example
The description only actually of property embodiment be it is illustrative, never as to the utility model and its application or any limit used
System.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
The every other embodiment obtained, fall within the protection scope of the utility model.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the utility model.As used herein, unless the context clearly indicates otherwise, otherwise singular
Form be also intended to include plural form, additionally, it should be understood that, when in the present specification use term "comprising" and/or
When " comprising ", existing characteristics, step, operation, device, component and/or their combination are indicated.
Unless specifically stated otherwise, positioned opposite, the digital table of the component and step that otherwise illustrate in these embodiments
Up to the unlimited the scope of the utility model processed of formula and numerical value.Simultaneously, it should be clear that for ease of description, each shown in attached drawing
The size of a part is not to draw according to actual proportionate relationship.Skill known for person of ordinary skill in the relevant
Art, method and apparatus may be not discussed in detail, but in the appropriate case, and the technology, method and apparatus should be considered as awarding
Weigh part of specification.In shown here and discussion all examples, example should be construed as merely by appointing to occurrence
Property, not as limitation.Therefore, the other examples of exemplary embodiment can have different values.It should also be noted that similar
Label and letter similar terms are indicated in following attached drawing, therefore, once be defined in a certain Xiang Yi attached drawing, then with
In attached drawing afterwards do not need that it is further discussed.
In the description of the present invention, it should be understood that the noun of locality such as " front, rear, top, and bottom, left and right ", " laterally,
Vertically, vertically, it is horizontal " and " pushing up, bottom " etc. indicated by orientation or positional relationship be normally based on orientation or position shown in the drawings
Relationship is set, is merely for convenience of describing the present invention and simplifying the description, in the absence of explanation to the contrary, these nouns of locality
Do not indicate that and imply that signified device or element must have a particular orientation or be constructed and operated in a specific orientation, because
This should not be understood as the limitation to scope of protection of the utility model: the noun of locality " inside and outside " refers to the wheel relative to each component itself
Wide is inside and outside.
For ease of description, spatially relative term can be used herein, as " ... on ", " ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation of sign.It should be understood that spatially relative term is intended to comprising the orientation in addition to device described in figure
Except different direction in use or operation.For example, being described as if the device in attached drawing is squeezed " in other devices
It will be positioned as " under other devices or construction after part or construction top " or the device of " on other devices or construction "
Side " or " under its device or construction ".Thus, exemplary term " ... top " may include " ... top " and
" in ... lower section " two kinds of orientation.The device can also be positioned with other different modes and (is rotated by 90 ° or in other orientation), and
And respective explanations are made to the opposite description in space used herein above.
In addition, it should be noted that, limiting components using the words such as " first ", " second ", it is only for be convenient for
Corresponding components are distinguished, do not have Stated otherwise such as, there is no particular meanings for above-mentioned word, therefore should not be understood as to this
The limitation of utility model protection range.
Embodiment 1
In the present embodiment, prepare it is a kind of for detecting the double channel optical fiber surface plasmon resonance biosensor of human immunoglobulin(HIg), i.e., to
Detection antigen is human immunoglobulin(HIg), and the antibody of detection is goat anti-human immunoglobulin.
As shown in Figure 1, a kind of double channel optical fiber surface plasmon resonance biosensor, including reference channel 1 ' and sensing passage 2 ', reference
Channel 1 ' is connected with sense channel 2 ' by multimode fibre, in which:
Reference channel 1 ' includes the first photonic crystal fiber 1, and the length of the first photonic crystal fiber 1 is 0.5~2cm, the
Distinguish welding multimode fibre 2 in one photonic crystal fiber, 1 both ends；First photonic crystal fiber, 1 electroplate film 4, the thickness of silverskin 4
For 40~60nm；Reference channel 1 ' is used as blank control, detects the wavelength amount of movement as caused by non-specific adsorption；
Sensing passage 2 ' includes the second photonic crystal fiber 2, and the length of the second photonic crystal fiber 2 is 0.5~2cm, the
Distinguish welding multimode fibre 3,2 surface gold-plating film 5 of the second photonic crystal fiber, the thickness of golden film 5 in the both ends of two photonic crystal fibers 2
Degree is 40~60nm；5 surface of golden film fixes graphene oxide film 6, graphene oxide film by Covalent bonding together method
With a thickness of 0.2~0.6nm, solidification has 7 film of antibody-goat anti-human immunoglobulin on graphene oxide film 6, utilizes oxidation
Graphene film 6 improves the fixed efficiency of antibody-goat anti-human immunoglobulin 7；Antigen-to be detected is human immunoglobulin(HIg) 8
It is fixed by gold nanoparticle 9, the diameter of gold nanoparticle is 10~50nm, antibody-goat anti-human immunoglobulin 7 in detection process
It is the combination of human immunoglobulin(HIg) 8 with antigen-, so that surface is fixed with the gold nanoparticle 9 that antigen-is human immunoglobulin(HIg) 8 and consolidates
It is scheduled on 7 film of antibody-goat anti-human immunoglobulin, generates coupling effect between golden film 5 and gold nanoparticle 9.
As shown in Fig. 2, the sensor-based system formed by above-mentioned double channel optical fiber surface plasmon resonance biosensor, including double channel optical fiber
Surface plasmon resonance biosensor A, system is using multimode fibre as optical path, i.e., multimode fibre is optical path D, double channel optical fiber surface plasmon resonance biosensor
The input terminal connection spectrum of A is the wideband light source B of visible light wave range, and double channel optical fiber surface plasmon resonance biosensor A output end connects light
Spectrometer C, spectrometer C are connected to computer G by data-interface, and double channel optical fiber surface plasmon resonance biosensor A is placed in closed tubulose
In xylometer to be checked, xylometer to be checked is glass tube H in the present embodiment, and the both ends glass tube H are provided with injection port E and outlet F,
Solution to be detected is entered by the injection port E of glass tube H, is flowed out from outlet F, so that double channel optical fiber SPR biology be made to pass
Sensor A is placed in solution to be detected.Glass tube H has fixed bracket I, and fixed bracket I support glass pipe H is placed in fixed bit
It sets.
The method for preparing above-mentioned double channel optical fiber surface plasmon resonance biosensor, steps are as follows:
(1) double channel optical fiber sensor is prepared
The outer diameter of the first photonic crystal fiber 1 and the second photonic crystal fiber 2 of sensing passage and reference channel is all 125
μm, the airport comprising the arrangement of 5 layers of regular hexagon, air bore dia is 4.8 μm, and air pitch of holes is 7.7 μm, and the first photon is brilliant
The length of body optical fiber 1 and the second photonic crystal fiber 2 is all 1cm, by the first photonic crystal fiber 1 and the second photonic crystal fiber
Welding multimode fibre 3 is distinguished by heat sealing machine in 2 both ends, and the outer diameter of multimode fibre 3 is 125 μm, and core diameter is 62.5 μm, the
One photonic crystal fiber 1, the second photonic crystal fiber 2 and multimode fibre 3 are silica glass material, the strength of discharge of heat sealing machine
For 130mW, discharge time 3000ms, fusion temp is 2000 DEG C；
(2) reference channel is prepared
By silver mirror reaction to 1 electroplate film 4 of the first photonic crystal fiber, silverskin 4 is with a thickness of 50nm；
(3) sensing passage is prepared
I gold-plated film
Second photonic crystal fiber 2 is put into vacuum ionic beam sputtering instrument, to 2 surface gold-plating of the second photonic crystal fiber
Film 5, the electric current of vacuum ionic beam sputtering instrument are 7mA, and the time is 2 minutes, and golden film 5 is with a thickness of 50nm；
II fixes graphene oxide film
Second photonic crystal fiber 2 of gold-plated film is immersed to the 4- aminothiophenol for filling that 20mL concentration is 0.5mmol/L
Ethanol solution culture dish in, distilled water flushing is used after 24 hours, 4- aminothiophenol molecule can pass through Au-S covalent bond
It is connect with golden film, and forms amido (- NH in golden film outer surface2)；Second photonic crystal fiber 2 is fixed on glass slide,
Taking concentration is the graphene oxide dispersion 0.5mL of 0.5mg/mL, submerges the second photonic crystal fiber 2, and is put into 40 DEG C of perseverance
In incubator, after forty minutes, graphene oxide dispersion evaporation is realized by physical evaporation method in the fixed oxidation in 5 surface of golden film
Graphene film 6, graphene oxide film 6 is with a thickness of 0.5nm；
III sessile antibody-goat anti-human immunoglobulin
Second photonic crystal fiber 2 is immersed in the 1- ethyl-(3- dimethylaminopropyl) that 1mL concentration is 0.4mol/L
In the mixed solution for the n-hydroxysuccinimide that phosphinylidyne diimmonium salt hydrochlorate and 1mL concentration are 0.1mol/L (1:1 mixing), 20
Distilled water flushing is clean after minute；Then the second photonic crystal fiber 2 is immersed into antibody-sheep that 500 μ L concentration are 200 μ g/mL
In 7 solution of human immunoglobulins, impregnates 1 hour, thereby realize antibody-goat anti-human immunoglobulin 7 in graphite oxide
The fixation on 6 surface of alkene film；
IV gold nanoparticle immobilized antigen-human immunoglobulin(HIg)
By 8 solution of antigen-human immunoglobulin(HIg) of various concentration be added to volume be 10mL, concentration 1000ppm, diameter
For antigen-human immunoglobulin(HIg) 8 is fixed on Jenner's grain of rice by electrostatic self-assembled in 9 dispersion liquid of gold nanoparticle of 10nm
The surface of son 9；
(4) antigen is detected
At 25 DEG C of temperature, the antigen-people fixed by gold nanoparticle 9 is added to double channel optical fiber spr sensor and is immunized
8 solution of globulin, antigen-human immunoglobulin(HIg) 8 that antibody-goat anti-human immunoglobulin 7 will be fixed with gold nanoparticle 9 are special
The opposite sex combines, and causes the movement of resonant wavelength, people's immunoglobulin of various concentration is realized according to the amount of movement of wavelength
Detection, the corresponding wavelength amount of movement of the human immunoglobulin(HIg) of various concentration is as shown in figure 5, from figure 5 it can be seen that sensing passage
2 ' are used for bio-sensing, and reference channel 1 ' is used as blank control, detect non-specific adsorption, the wavelength amount of movement of sensing passage 2 '
The wavelength amount of movement for subtracting reference channel 1 ' has just obtained practical antibody (goat anti-human immunoglobulin) and antigen (people's immune globulin
It is white) between specific binding caused by resonant wavelength drift value.
The refractive index and bio-sensing characteristic of the utility model double channel optical fiber surface plasmon resonance biosensor of above-mentioned preparation are surveyed
Examination:
In order to study the refractive index sensing performance for the double channel optical fiber spr sensor that the utility model is proposed, by the biography
Sensor is accessed using multimode fibre as the sensor-based system of optical path, deuterium-halogen lamp that input terminal is 215nm to 2500nm using wave-length coverage
As light source, resonance spectrum is detected using marine optics spectrometer, is then immersed the double channel optical fiber spr sensor different
In the sodium chloride brine of refractive index, resonance spectrum as shown in figure 3, with refractive index increase, double channel optical fiber spr sensor
Resonant wavelength drift about to the right.The sensitivity of sensor can be expressed as the shifted by delta λ of resonance wave crestpWith sample to be tested refractive index
Change Δ naRatio, i.e.,
Fig. 4 is the refractive index sensitivity matched curve for the double channel optical fiber spr sensor that the utility model is proposed, according to
The slope of matched curve shown in Fig. 4, can be obtained the utility model proposes double channel optical fiber spr sensor refractive index it is sensitive
Degree reaches 13592nm/RIU, the linearity 0.98896, and the refractive index sensitivity of reference channel 1 ' is 2524nm/RIU, the linearity
It is 0.9659, the refractive index sensitivity of sensing passage 2 ' is 5.4 times of the only refractive index sensitivity of the reference channel 1 ' of plating silverskin,
This is because sensing passage 2 ' is coated with golden film and is fixed with graphene oxide film, graphene oxide has efficient electron transfer
Rate enhances the electromagnetic field intensity of sensor surface and the interaction of external agency, and the refractive index for improving sensor is sensitive
Degree illustrates the important function that the double channel optical fiber spr sensor that the utility model is proposed has raising detection sensitivity.
The utility model double channel optical fiber surface plasmon resonance biosensor utilizes channel structure, reference channel as blank control,
Wavelength amount of movement caused by non-specific adsorption is detected, while sensing passage utilizes golden film and Jenner as bio-sensing channel
Coupling effect between rice corpuscles enhances local electric field intensity to improve the detection sensitivity of sensor, in the photon for being coated with golden film
Graphene oxide film is fixed by Covalent bonding together method in crystal optical fibre surface, is improved using graphene oxide film anti-
The antigen that the fixed efficiency of body (goat anti-human immunoglobulin) (goat anti-human immunoglobulin) at antibody and is fixed by gold nanoparticle
Specific binding between (human immunoglobulin(HIg)) causes resonant wavelength to drift about, and realizes antigen according to the amount of movement of resonant wavelength
(human immunoglobulin(HIg)) high-sensitivity measurement, while referring to the accuracy of detection.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should
Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of
Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new
The range of each embodiment technical solution of type.
Claims (9)
1. a kind of double channel optical fiber surface plasmon resonance biosensor, which is characterized in that including reference channel and sensing passage, reference channel
It is connected with sense channel by multimode fibre, in which:
Reference channel includes the first photonic crystal fiber, and welding multimode fibre, the first light are distinguished in the first photonic crystal fiber both ends
Photonic crystal fiber electroplate film；Reference channel detects the wavelength amount of movement as caused by non-specific adsorption as blank control；
Sensing passage includes the second photonic crystal fiber, and welding multimode fibre is distinguished at the both ends of the second photonic crystal fiber, and second
Graphene oxide film is fixed on photonic crystal fiber surface gold-plating film, golden film surface, and solidifying on graphene oxide film has to life
Object molecule or chemical component have the antibody film layer of selective absorbing, and the antigen corresponding to antibody is fixed by gold nanoparticle, examine
Antibody and antigen binding during survey so that the gold nanoparticle that surface is fixed with antigen is fixed on antibody film, golden film with
Coupling effect is generated between gold nanoparticle.
2. a kind of double channel optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the reference is logical
The length of second photonic crystal fiber of first photonic crystal fiber and sensing passage in road is 0.5~2cm.
3. a kind of double channel optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the reference is logical
First photon crystal surface in road plates silverskin, and silver film thickness is 40~60nm.
4. a kind of double channel optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the sensing is logical
The second photonic crystal fiber surface gold-plating film in road, golden film is with a thickness of 40~60nm.
5. a kind of double channel optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the sensing is logical
The fixed graphene oxide film in the golden film surface in road, it is thin that graphene oxide is fixed by Covalent bonding together method in golden film surface
Film improves the fixed efficiency of antibody using graphene oxide film.
6. a kind of double channel optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the sensing is logical
The fixed graphene oxide film in the golden film surface in road, graphene oxide film with a thickness of 0.2~0.6nm.
7. a kind of double channel optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the sensing is logical
The diameter of the gold nanoparticle in road is 10~50nm.
8. the bilateral that double channel optical fiber surface plasmon resonance biosensor is formed as described in any one of claim 1 to 7 claim
Road optical fiber sensor-based system, which is characterized in that including using multimode fibre as the double channel optical fiber spr sensor of optical path, broadband light
Source and spectrometer, the optical transport that double channel optical fiber spr sensor input terminal is issued wideband light source by multimode fibre optical path is extremely
Double channel optical fiber spr sensor, double channel optical fiber spr sensor output end connect spectrometer, and spectrometer is connected by data-interface
It is connected to computer, double channel optical fiber surface plasmon resonance biosensor is placed in the solution of biomolecule to be detected.
9. the double channel optical fiber SPR sensorgram system that double channel optical fiber surface plasmon resonance biosensor according to claim 8 is formed,
It is characterized in that, the double channel optical fiber SPR sensorgram system is placed in closed tubulose xylometer to be checked, xylometer two to be checked
End is provided with injection port and outlet；Tubulose xylometer to be checked has fixed bracket, and fixed bracket support tubulose is to be checked
Xylometer is placed in fixed position.
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| CN109060727A (en) * | 2018-08-22 | 2018-12-21 | 东北大学 | A kind of double channel optical fiber surface plasmon resonance biosensor |
| CN111272703A (en) * | 2020-03-19 | 2020-06-12 | 南京信息工程大学 | Array type multi-channel optical fiber sensor and preparation method thereof |
| CN113406324A (en) * | 2021-06-30 | 2021-09-17 | 吉林大学 | S-shaped optical fiber cone immunosensor, preparation method and application thereof |
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| CN109959635A (en) * | 2019-02-22 | 2019-07-02 | 东北大学 | A kind of optical fiber SPR sensor based on field coupling |
| CN110231309A (en) * | 2019-04-15 | 2019-09-13 | 厦门大学 | A method of utilizing the medium prism structure and its sensing of graphene |
| CN110132896A (en) * | 2019-05-06 | 2019-08-16 | 山西大学 | The micro optical fiber biosensor of markers for breast cancer in a kind of quick detection serum |
| CN110108384A (en) * | 2019-05-16 | 2019-08-09 | 东北大学 | Based on hollow-core fiber surface plasma resonance in fine formula temperature sensor and detection method |
| CN110672564A (en) * | 2019-10-22 | 2020-01-10 | 重庆理工大学 | Nano-gold shell LSPR (localized surface plasmon resonance) optode biosensor of graphene oxide fiber bragg grating |
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| US20120081703A1 (en) * | 2009-05-07 | 2012-04-05 | Nant Holdings Ip, Llc | Highly Efficient Plamonic Devices, Molecule Detection Systems, and Methods of Making the Same |
| US10324034B2 (en) * | 2009-10-20 | 2019-06-18 | National Chung Cheng University | Self-referencing localized plasmon resonance sensing device and system thereof |
| US9223064B2 (en) * | 2010-05-28 | 2015-12-29 | The Regents Of The University Of Michigan | Photonic crystal-metallic structures and applications |
| CN103398982A (en) * | 2013-07-25 | 2013-11-20 | 天津大学 | Method and sensor for testing surface plasmon resonance of pohotonic crystal fibers |
| ES2553027B1 (en) * | 2014-06-03 | 2016-09-13 | Consejo Superior De Investigaciones Cientificas | A SYSTEM FOR BIODETECTION APPLICATIONS |
| US9423345B2 (en) * | 2014-06-24 | 2016-08-23 | International Business Machines Corporation | Chemical sensors based on plasmon resonance in graphene |
| CN106556575B (en) * | 2015-09-24 | 2019-03-01 | 深圳先进技术研究院 | Online two-beam interference type optic fibre refractive index sensor and refractive index detection system |
| CN106556574B (en) * | 2015-09-24 | 2018-12-07 | 深圳先进技术研究院 | Online two-beam interference type optic fibre refractive index sensor and refractivity tester |
| CN106066313A (en) * | 2016-05-25 | 2016-11-02 | 哈尔滨工程大学 | Distributed surface plasma resonance optical fiber sensor and the method for measuring refractive indexes of liquid |
| CN105866070A (en) * | 2016-05-27 | 2016-08-17 | 哈尔滨工程大学 | Distributed liquid refractive index sensing device based on optical fiber surface plasma resonance |
| CN106596474B (en) * | 2016-12-05 | 2020-11-06 | 重庆三峡学院 | Three-channel SPR sensor based on seven-core optical fiber |
| CN109060727B (en) * | 2018-08-22 | 2024-01-26 | 东北大学 | Dual-channel optical fiber SPR biosensor |
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| CN109060727A (en) * | 2018-08-22 | 2018-12-21 | 东北大学 | A kind of double channel optical fiber surface plasmon resonance biosensor |
| CN109060727B (en) * | 2018-08-22 | 2024-01-26 | 东北大学 | Dual-channel optical fiber SPR biosensor |
| CN111272703A (en) * | 2020-03-19 | 2020-06-12 | 南京信息工程大学 | Array type multi-channel optical fiber sensor and preparation method thereof |
| CN113406324A (en) * | 2021-06-30 | 2021-09-17 | 吉林大学 | S-shaped optical fiber cone immunosensor, preparation method and application thereof |
| CN113406324B (en) * | 2021-06-30 | 2023-01-24 | 吉林大学 | S-shaped optical fiber cone immunosensor, preparation method and application thereof |
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