CN208752007U - A kind of high sensitivity optical fiber surface plasmon resonance biosensor - Google Patents
A kind of high sensitivity optical fiber surface plasmon resonance biosensor Download PDFInfo
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- CN208752007U CN208752007U CN201821482943.2U CN201821482943U CN208752007U CN 208752007 U CN208752007 U CN 208752007U CN 201821482943 U CN201821482943 U CN 201821482943U CN 208752007 U CN208752007 U CN 208752007U
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
Abstract
The utility model proposes a kind of high sensitivity optical fiber surface plasmon resonance biosensors.The utility model mainly improves the detection sensitivity of sensor using the coupling effect enhancing local electric field intensity between golden film and gold nanoparticle, graphene oxide film is fixed by Covalent bonding together method on the surface for the Photonic Crystal Fiber Sensor for being coated with golden film, the fixed efficiency of antibody is improved using graphene oxide film, antibody and cause resonant wavelength to drift about by the specific binding between antigen that gold nanoparticle is fixed, the measurement of antigen high sensitivity and low detection limit is realized according to the amount of movement of resonant wavelength.The utility model solves the problems, such as that the sensitivity of existing fiber surface plasmon resonance biosensor is lower, has broad application prospects in immunoassay and the detection of low concentration or small biological molecule.
Description
Technical field
The utility model relates to biosensor technology fields, and in particular to surface plasmon resonance biosensor more particularly to a kind of high
Sensitivity optical fiber biosensor.
Background technique
Surface Plasmon Resonance (SPR), Chinese are surface plasma body resonant vibration, it is a kind of normal
The optical phenomena seen, when it refers to that the incident light of a certain specific wavelength in optical waveguide is irradiated to metal (such as gold or silver) film, light
The plasma wave that wave will be generated with metal surface resonates, referred to as surface plasma body resonant vibration (SPR) effect.SPR
Effect can make the energy of reflected light fall sharply, so that resonance trough is formed, meanwhile, SPR is very sensitive to extraneous refractive index, when
When surveyed solution concentration change, i.e. extraneous solution refraction index changing, the resonance trough of SPR will be moved, and therefore, this is practical
The novel offset by detection SPR resonance trough achievees the purpose that detect biological sample concentration.
Surface plasma body resonant vibration (SPR) biosensor in biology and is changed due to its highly sensitive and biocompatibility
It learns in detection and causes great concern.According to the interaction between surface plasma wave and surrounding biologic molecule, can lead
The drift for causing resonance angle or resonant wavelength, thus can realize the detection of external biomolecule.With traditional SPR biology based on prism
Sensor is compared, and optical fiber biosensor has production simply, at low cost, sensing arrangement miniaturization and electromagnetism interference
Characteristic.However, traditional SPR bio-sensing relies primarily on golden film to realize detection, lack sufficiently high sensitivity to detect more
The biomolecule of low relative molecular mass facilitates to expand in this way therefore, there is still a need for further increasing the sensitivity of sensor
The application range of sensor.
Utility model content
Sensitivity in order to solve the problems, such as existing fiber surface plasmon resonance biosensor is lower, and the utility model proposes one kind
High sensitivity optical fiber surface plasmon resonance biosensor.The utility model mainly utilizes the coupling effect between golden film and gold nanoparticle to increase
Strong local electric field intensity improves the detection sensitivity of sensor, logical on the surface for the Photonic Crystal Fiber Sensor for being coated with golden film
Covalent bonding together method is crossed to fix graphene oxide film, the fixed efficiency of antibody is improved using graphene oxide film,
Antibody and resonant wavelength is caused to drift about by the specific binding between antigen that gold nanoparticle is fixed, according to the shifting of resonant wavelength
Momentum come realize antigen high sensitivity and it is low detection limit measurement.
To achieve the above object, the utility model uses following technical scheme:
A kind of high sensitivity optical fiber surface plasmon resonance biosensor, including photonic crystal fiber, photonic crystal fiber both ends are melted respectively
Connect multimode fibre, photonic crystal fiber surface gold-plating film, the fixed graphene oxide film in golden film surface, on graphene oxide film
Solidifying has the antibody film layer for having selective absorbing to biomolecule or chemical component, and the antigen corresponding to antibody is by Jenner's grain of rice
Son is fixed, antibody and antigen binding in detection process, so that the gold nanoparticle that surface is fixed with antigen is fixed on antibody film
On, coupling effect is generated between golden film and gold nanoparticle.
Further, the fixed graphene oxide film in the golden film surface, golden film surface pass through Covalent bonding together method
Graphene oxide film is fixed, improves the fixed efficiency of antibody using graphene oxide film.
Further, the length of the photonic crystal fiber is 0.5~2cm.
Further, the photonic crystal fiber surface gold-plating film, golden film with a thickness of 40~60nm.
Further, the fixed graphene oxide film in the golden film surface, graphene oxide film with a thickness of 0.2~
0.6nm。
Further, the diameter of the gold nanoparticle is 10~50nm.
The principles of the present invention are as follows:
The utility model is mainly mentioned using the coupling effect enhancing local electric field intensity between golden film and gold nanoparticle
The detection sensitivity of high sensor, the surface for the Photonic Crystal Fiber Sensor for being coated with golden film by Covalent bonding together method come
Fixed graphene oxide film, improves the fixed efficiency of antibody using graphene oxide film, antibody and by gold nanoparticle
Specific binding between fixed antigen causes resonant wavelength to drift about, and antigen Gao Ling is realized according to the amount of movement of resonant wavelength
The measurement of sensitivity and low detection limit.
The preparation method of above-mentioned high sensitivity optical fiber surface plasmon resonance biosensor, steps are as follows:
(1) Photonic Crystal Fiber Sensor is prepared
Welding multimode fibre is distinguished by heat sealing machine in photonic crystal fiber both ends, the length of photonic crystal fiber is 0.5
~2cm, strength of discharge when welding are 50~100mW, and discharge current is 3000~5000mA, and fusion temp is 2000~3000
℃；
(2) gold-plated film
The Photonic Crystal Fiber Sensor of preparation is put into vacuum ionic beam sputtering instrument, to sensor surface gold-plated film,
The electric current of vacuum ionic beam sputtering instrument be 5~7mA, the time be 2~4 minutes, golden film with a thickness of 40~60nm；
(3) fixed graphene oxide film
Photonic Crystal Fiber Sensor after gold-plated film is immersed to the 4- aminothiophenol ethanol solution of 0.5~2mmol/L
In 6~24 hours, to carry out golden film surface amination, 4- aminothiophenol molecule can be connect by Au-S covalent bond with golden film,
And by amido (- NH2) be left outside, with further in conjunction with the epoxy group of graphene oxide；After distilled water flushing, photon
Crystal optical fibre sensor immerses in the graphene oxide water solution of 0.05~1mg/mL, and the graphene oxide water solution is put into
Temperature is in 30~60 DEG C of insulating box, 20~after sixty minutes, and graphene oxide dispersion evaporation passes through physical evaporation method
Realize in the fixed graphene oxide film in golden film surface, graphene oxide film with a thickness of 0.2~0.6nm；
(4) sessile antibody
Photonic Crystal Fiber Sensor is immersed in 1- ethyl-(the 3- diformazan that 0.5~1mL concentration is 0.1~0.4mol/L
Base aminopropyl) phosphinylidyne diimmonium salt hydrochlorate and 0.5~1mL concentration be 0.1~0.4mol/L n-hydroxysuccinimide
In mixed solution, 15~after sixty minutes, distilled water flushing is clean；Then it is entered in antibody-solutions to be fixed and is resisted
Body is fixed；
(5) gold nanoparticle immobilized antigen
Antigen to be detected is added to the golden nanometer particle dispersion liquid that concentration is 500~1000ppm, diameter is 10~50nm
In, antigen is fixed on to the surface of gold nanoparticle by electrostatic self-assembled；
(6) antigen is detected
At 25~37 DEG C of temperature, the people fixed by gold nanoparticle is sequentially added to Photonic Crystal Fiber Sensor surface
Immunoglobulin solution causes resonance wave journey by raft down the Yangtze River according to antibody and by the specific binding between antigen that gold nanoparticle is fixed
It moves, realizes the detection of antigen.
The sensor-based system formed by above-mentioned high sensitivity optical fiber surface plasmon resonance biosensor, which is characterized in that including with multimode light
Fibre is the high sensitivity optical fiber surface plasmon resonance biosensor of optical path, and input terminal connects the wideband light source that spectrum is visible light wave range,
Output end connects broadband light spectrometer, and broadband light spectrometer is connected to computer by data-interface, and high sensitivity optical fiber SPR biology passes
Sensor is placed in biomolecule solution to be detected.
Further, the high sensitivity optical fiber surface plasmon resonance biosensor is placed in closed tubulose xylometer to be checked, to
Detection container both ends are provided with injection port and outlet.
Further, tubulose xylometer to be checked has fixed bracket, and fixed bracket supports tubulose appearance to be detected
Device is placed in fixed position.
The beneficial effect of the utility model compared with prior art is:
1, enhance local electric field intensity using the coupling effect between golden film and gold nanoparticle, so that the photonic crystal
Optical fiber biosensor has the advantages that highly sensitive and low detection limit；
2, graphene oxide has excellent bio-sensing characteristic, has biocompatibility and bigger serface, Ke Yigeng
The fixation of antibody is realized well；
3, by Covalent bonding together mode in the fixed graphene oxide membrane in golden film surface, using electrostatic self-assembled method anti-
Original is fixed on gold nanoparticle surface, and graphene oxide promotes the transfer of electronics between golden film and gold nanoparticle, is conducive to increase
Forceful electric power field intensity improves detection sensitivity；
4, photonic crystal fiber itself has to temperature-insensitive characteristic, it is possible to reduce the fluctuation of ambient temperature is to inspection
Survey impacts, and sensor is with good stability and repeated；
In conclusion the utility model solves the problems, such as that existing fiber surface plasmon resonance biosensor sensitivity is lower, immune
It is had broad application prospects in analysis and the detection of low concentration or small biological molecule.
Detailed description of the invention
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The some embodiments recorded in application, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings.
Fig. 1 is that the utility model proposes the sensors of one of embodiment 1 high sensitivity optical fiber surface plasmon resonance biosensor
Structural schematic diagram；
Fig. 2 is that the utility model proposes the signals of the detection system of the high sensitivity optical fiber surface plasmon resonance biosensor in embodiment 1
Figure；
Fig. 3 is optical fiber biosensor structure schematic diagram in the prior art；
Fig. 4 is that high sensitivity optical fiber surface plasmon resonance biosensor is reflected in extraneous sodium chloride solution in the utility model embodiment 1
Resonance spectrum when rate changes from 1.3320 to 1.3361；
Fig. 5 is the refractive index and wave of high-sensitivity photonic crystal fiber surface plasmon resonance biosensor in the utility model embodiment 1
Long matched curve；
Fig. 6 is that high-sensitivity photonic crystal fiber surface plasmon resonance biosensor is applied to detection not in the utility model embodiment 1
With concentration human immunoglobulin(HIg) when corresponding wavelength amount of movement；
In figure: 1, photonic crystal fiber, 2, multimode fibre, 3, golden film, 4, graphene oxide film, 5, antibody-goat-anti people
Immunoglobulin, 6, antigen-human immunoglobulin(HIg), 7, gold nanoparticle；A, high sensitivity optical fiber surface plasmon resonance biosensor, B, width
Band light source, C, spectrometer, D, multimode fibre optical path, E, injection port, F, outlet, G, computer, H, glass tube, I, bracket；1',
Single mode optical fiber, 2 ', multimode fibre, 3 ', golden film, 4 ', antibody-goat anti-human immunoglobulin, 5 ', antigen-human immunoglobulin(HIg).
Specific embodiment
It should be noted that in the absence of conflict, the feature in the embodiments of the present invention and embodiment can
To be combined with each other.The utility model will be described in detail below with reference to the accompanying drawings and embodiments.
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is only the utility model a part of the embodiment, instead of all the embodiments.Below at least one example
The description only actually of property embodiment be it is illustrative, never as to the utility model and its application or any limit used
System.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
The every other embodiment obtained, fall within the protection scope of the utility model.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the utility model.As used herein, unless the context clearly indicates otherwise, otherwise singular
Form be also intended to include plural form, additionally, it should be understood that, when in the present specification use term "comprising" and/or
When " comprising ", existing characteristics, step, operation, device, component and/or their combination are indicated.
Unless specifically stated otherwise, positioned opposite, the digital table of the component and step that otherwise illustrate in these embodiments
Up to the unlimited the scope of the utility model processed of formula and numerical value.Simultaneously, it should be clear that for ease of description, each shown in attached drawing
The size of a part is not to draw according to actual proportionate relationship.Skill known for person of ordinary skill in the relevant
Art, method and apparatus may be not discussed in detail, but in the appropriate case, and the technology, method and apparatus should be considered as awarding
Weigh part of specification.In shown here and discussion all examples, example should be construed as merely by appointing to occurrence
Property, not as limitation.Therefore, the other examples of exemplary embodiment can have different values.It should also be noted that similar
Label and letter similar terms are indicated in following attached drawing, therefore, once be defined in a certain Xiang Yi attached drawing, then with
In attached drawing afterwards do not need that it is further discussed.
In the description of the present invention, it should be understood that the noun of locality such as " front, rear, top, and bottom, left and right ", " laterally,
Vertically, vertically, it is horizontal " and " pushing up, bottom " etc. indicated by orientation or positional relationship be normally based on orientation or position shown in the drawings
Relationship is set, is merely for convenience of describing the present invention and simplifying the description, in the absence of explanation to the contrary, these nouns of locality
Do not indicate that and imply that signified device or element must have a particular orientation or be constructed and operated in a specific orientation, because
This should not be understood as the limitation to scope of protection of the utility model: the noun of locality " inside and outside " refers to the wheel relative to each component itself
Wide is inside and outside.
For ease of description, spatially relative term can be used herein, as " ... on ", " ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation of sign.It should be understood that spatially relative term is intended to comprising the orientation in addition to device described in figure
Except different direction in use or operation.For example, being described as if the device in attached drawing is squeezed " in other devices
It will be positioned as " under other devices or construction after part or construction top " or the device of " on other devices or construction "
Side " or " under its device or construction ".Thus, exemplary term " ... top " may include " ... top " and
" in ... lower section " two kinds of orientation.The device can also be positioned with other different modes and (is rotated by 90 ° or in other orientation), and
And respective explanations are made to the opposite description in space used herein above.
In addition, it should be noted that, limiting components using the words such as " first ", " second ", it is only for be convenient for
Corresponding components are distinguished, do not have Stated otherwise such as, there is no particular meanings for above-mentioned word, therefore should not be understood as to this
The limitation of utility model protection range.
Embodiment 1
In the present embodiment, prepare it is a kind of for detecting the high sensitivity optical fiber surface plasmon resonance biosensor of human immunoglobulin(HIg), i.e.,
Antigen to be detected is human immunoglobulin(HIg), and the antibody of detection is goat anti-human immunoglobulin.
As shown in Figure 1, a kind of high sensitivity optical fiber surface plasmon resonance biosensor, including photonic crystal fiber 1, photonic crystal light
The length of fibre 1 is 0.5~2cm, and welding multimode fibre 2,1 surface gold-plating of photonic crystal fiber are distinguished in 1 both ends of photonic crystal fiber
Film 3, golden film 3 with a thickness of 40~60nm；3 surface of golden film fixes graphene oxide film 4, oxygen by Covalent bonding together method
Graphite alkene film with a thickness of 0.2~0.6nm, antibody-goat anti-human immunoglobulin is improved using graphene oxide film 4
5 fixed efficiency；Solidification has 5 film of antibody-goat anti-human immunoglobulin on graphene oxide film 4, and antigen-to be detected is people
Immunoglobulin 6 is fixed by gold nanoparticle 7, and the diameter of gold nanoparticle is 10~50nm, antibody-goat-anti people in detection process
Immunoglobulin 5 and antigen-are the combination of human immunoglobulin(HIg) 6, so that surface is fixed with the gold that antigen-is human immunoglobulin(HIg) 6
Nanoparticle 7 is fixed on 5 film of antibody-goat anti-human immunoglobulin, is generated between golden film 3 and gold nanoparticle 7 and is coupled effect
It answers.
As shown in Fig. 2, the sensor-based system formed by above-mentioned high sensitivity optical fiber surface plasmon resonance biosensor, including high sensitivity
Optical fiber biosensor A, system is using multimode fibre as optical path, i.e., multimode fibre is optical path D, high sensitivity optical fiber SPR biology
The input terminal connection spectrum of sensors A is the wideband light source B of visible light wave range, high sensitivity optical fiber surface plasmon resonance biosensor A output
End connection broadband light spectrometer C, broadband light spectrometer C is connected to computer G by data-interface, and high sensitivity optical fiber SPR biology passes
Sensor A is placed in closed tubulose xylometer to be checked, and xylometer to be checked is glass tube H, the setting of the both ends glass tube H in the present embodiment
There are injection port E and outlet F, solution to be detected is entered by the injection port E of glass tube H, flowed out from outlet F, to make
High sensitivity optical fiber surface plasmon resonance biosensor A is placed in solution to be detected.Glass tube H has fixed bracket I, fixed bracket I support
Glass tube H is placed in fixed position.
The method for preparing above-mentioned high sensitivity optical fiber surface plasmon resonance biosensor, steps are as follows:
(1) fibre optical sensor is prepared
The outer diameter of photonic crystal fiber 1 is 125 μm, the airport comprising the arrangement of 5 layers of regular hexagon, and air bore dia is
4.8 μm, air pitch of holes is 7.7 μm, and the length of photonic crystal fiber 1 is 1cm, and the both ends of photonic crystal fiber 1 pass through welding
Machine distinguishes welding multimode fibre 2, and the outer diameter of multimode fibre 2 is 125 μm, and core diameter is 62.5 μm, photonic crystal fiber 1 and more
Mode fiber 2 is silica glass material, and the strength of discharge of heat sealing machine is 130mW, discharge time 3000ms, fusion temp 2000
℃；
(2) gold-plated film
Photonic Crystal Fiber Sensor prepared by step (1) is put into vacuum ionic beam sputtering instrument, to sensor surface
Gold-plated film 3, the electric current of vacuum ionic beam sputtering instrument are 7mA, and the time is 2 minutes, and golden film 3 is with a thickness of 50nm；
(3) fixed graphene oxide film
The ethyl alcohol that Photonic Crystal Fiber Sensor immersion is filled the 4- aminothiophenol that 20mL concentration is 0.5mmol/L is molten
In the culture dish of liquid, distilled water flushing is used after 24 hours, 4- aminothiophenol molecule can connect by Au-S covalent bond and golden film
It connects, and forms amido (- NH in golden film outer surface2)；Photonic Crystal Fiber Sensor is fixed on glass slide, takes the concentration to be
The graphene oxide dispersion 0.5mL of 0.5mg/mL submerges Photonic Crystal Fiber Sensor, and is put into 40 DEG C of insulating box,
After forty minutes, graphene oxide dispersion is evaporated, and is realized by physical evaporation method thin in the fixed graphene oxide in golden film surface
Film, graphene oxide film is with a thickness of 0.5nm；
(4) sessile antibody-goat anti-human immunoglobulin
Photonic Crystal Fiber Sensor is immersed in 1- ethyl-(the 3- dimethylamino third that 1mL concentration is 0.4mol/L
Base) phosphinylidyne diimmonium salt hydrochlorate and 1mL concentration be 0.1mol/L n-hydroxysuccinimide mixed solution in (1:1 is mixed
Close), distilled water flushing is clean after twenty minutes；Then Photonic Crystal Fiber Sensor is immersed to the 500 μ L bought from biotech firm
Concentration is to impregnate 1 hour in antibody-goat anti-human immunoglobulin's solution of 200 μ g/mL, thereby realize antibody-goat-anti people
Fixation of the immunoglobulin 5 on 4 surface of graphene oxide film；
(5) gold nanoparticle immobilized antigen-human immunoglobulin(HIg)
Antigen-human immunoglobulin(HIg) solution is bought from biotech firm, by the antigen of various concentration-human immunoglobulin(HIg) solution
It is added to that volume is 10mL, concentration 1000ppm, diameter are to pass through electrostatic self-assembled in the golden nanometer particle dispersion liquid of 10nm
Human immunoglobulin(HIg) is fixed on to the surface of gold nanoparticle.
(6) antigen is detected
At 25 DEG C of temperature, the people fixed by gold nanoparticle is sequentially added to Photonic Crystal Fiber Sensor surface and is immunized
Globulin solution, the human immunoglobulin(HIg) specific binding that goat anti-human immunoglobulin will be fixed with gold nanoparticle, causes
The detection of people's immunoglobulin of various concentration is realized in the movement of resonant wavelength on spectrometer according to the amount of movement of wavelength,
The corresponding wavelength amount of movement of the human immunoglobulin(HIg) of various concentration is as shown in Figure 6.
The utility model high sensitivity optical fiber surface plasmon resonance biosensor and existing fiber surface plasmon resonance biosensor of above-mentioned preparation
Refractive index sensing characteristic test:
In order to study that the utility model proposed in successively gold-plated film and the fixation from inside to outside of photonic crystal fiber surface
The refractive index sensing performance of sensor after graphene oxide film accesses the sensor using multimode fibre as the sensing system of optical path
System, it is deuterium-halogen lamp of 215nm to 2500nm as light source that input terminal, which uses wave-length coverage, is examined using marine optics spectrometer
Resonance spectrum is surveyed, the sensor is then immersed into variations in refractive index range in the sodium chloride brine of 1.3320-1.3361 respectively
In, resonance spectrum as shown in figure 4, with refractive index increase, resonant wavelength drifts about to the right.The sensitivity of sensor can indicate
For the shifted by delta λ for the wave crest that resonatespWith the change Δ n of sample to be tested refractive indexaRatio, i.e.,
Under the variations in refractive index range, resonant wavelength floats to the right 734nm from 682nm.Fig. 5 is the utility model institute
The refractive index sensitivity matched curve of the Photonic Crystal Fiber Sensor of proposition, the slope of matched curve according to figure 5 can
The refractive index sensitivity for obtaining the high sensitivity optical fiber surface plasmon resonance biosensor of the utility model is 12357nm/RIU.
As shown in figure 3, optical fiber biosensor is using 1 ' both ends of single mode optical fiber difference welding multimode light in the prior art
Fibre 2 ', 1 ' outer diameter of single mode optical fiber are 125 μm, and core diameter is 8.2 μm, and the outer diameter of multimode fibre 2 ' is 125 μm, and core diameter is
62.5 μm, the golden film 3 ' that a layer thickness is 50nm is plated on 1 ' surface of single mode optical fiber, and the refractive index sensitivity of the sensor is 2500nm/
RIU。
It can thus be seen that sensitivity and resolution of the microstructure fiber sensor of the utility model than general sensor
Rate is higher.
The utility model is mainly mentioned using the coupling effect enhancing local electric field intensity between golden film and gold nanoparticle
The detection sensitivity of high sensor, the surface for the Photonic Crystal Fiber Sensor for being coated with golden film by Covalent bonding together method come
Fixed graphene oxide film, the fixed efficiency of antibody (goat anti-human immunoglobulin) is improved using graphene oxide film,
Specific binding between antibody (goat anti-human immunoglobulin) and the antigen (human immunoglobulin(HIg)) fixed by gold nanoparticle
Cause resonant wavelength to drift about, antigen (human immunoglobulin(HIg)) high sensitivity and low detection are realized according to the amount of movement of resonant wavelength
The measurement of limit.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should
Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of
Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new
The range of each embodiment technical solution of type.
Claims (9)
1. a kind of high sensitivity optical fiber surface plasmon resonance biosensor, which is characterized in that including photonic crystal fiber, photonic crystal fiber
Welding multimode fibre, photonic crystal fiber surface gold-plating film are distinguished in both ends, and the fixed graphene oxide film in golden film surface aoxidizes stone
Solidifying on black alkene film has the antibody film layer for having selective absorbing to biomolecule or chemical component, the antigen corresponding to antibody
It is fixed by gold nanoparticle, antibody and antigen binding in detection process, so that the gold nanoparticle that surface is fixed with antigen is fixed
On antibody film, coupling effect is generated between golden film and gold nanoparticle.
2. a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the golden film
Graphene oxide film is fixed on surface, and golden film surface fixes graphene oxide film by Covalent bonding together method, utilizes oxygen
Graphite alkene film improves the fixed efficiency of antibody.
3. a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the photon
The length of crystal optical fibre is 0.5~2cm.
4. a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the photon
Crystal optical fibre surface gold-plating film, golden film with a thickness of 40~60nm.
5. a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the golden film
Surface fix graphene oxide film, graphene oxide film with a thickness of 0.2~0.6nm.
6. a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 1, which is characterized in that the Jenner
The diameter of rice corpuscles is 10~50nm.
7. by a kind of described in any item sensor-based systems that high sensitivity optical fiber surface plasmon resonance biosensor is formed of claim 1~6,
It is characterised in that it includes using multimode fibre as the high sensitivity optical fiber surface plasmon resonance biosensor of optical path, input terminal connection spectrum is
The wideband light source of visible light wave range, output end connect broadband light spectrometer, and broadband light spectrometer is connected to calculating by data-interface
Machine, high sensitivity optical fiber surface plasmon resonance biosensor are placed in biomolecule solution to be detected.
8. the sensor-based system that a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 7 is formed, feature exist
In the high sensitivity optical fiber surface plasmon resonance biosensor is placed in closed tubulose xylometer to be checked, and xylometer both ends to be checked are set
It is equipped with injection port and outlet.
9. the sensor-based system that a kind of high sensitivity optical fiber surface plasmon resonance biosensor according to claim 8 is formed, feature exist
In tubulose xylometer to be checked has fixed bracket, and fixed bracket support tubulose container to be detected is placed in fixed position.
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| CN109085140A (en) * | 2018-08-22 | 2018-12-25 | 东北大学 | A kind of high sensitivity optical fiber surface plasmon resonance biosensor |
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| CN111413298A (en) * | 2020-05-27 | 2020-07-14 | 深圳市天安至远传感科技有限公司 | Optical fiber surface plasma resonance sensor, preparation method and detection system |
| CN112033932B (en) * | 2020-09-07 | 2021-09-07 | 科竟达生物科技有限公司 | Local surface plasmon resonance biochip, manufacturing method thereof, biosensing system comprising local surface plasmon resonance biochip and application of biosensing system |
| CN113155744B (en) * | 2021-04-26 | 2022-03-11 | 广东长光中科生物科技有限公司 | Method for quantitatively detecting target protein in serum by micro-nano optical fiber coupler label-free biosensing |
| CN113280843A (en) * | 2021-05-20 | 2021-08-20 | 山东大学 | Graphene sensitive tilt-increasing grating optical fiber SPR sensor and analysis method and application |
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| CN105651840B (en) * | 2016-04-01 | 2017-12-26 | 商丘师范学院 | A kind of para-immunity electrochemical sensor for detecting amyloid beta oligomer and preparation method thereof |
| CN105891155B (en) * | 2016-04-08 | 2019-05-07 | 山东大学 | A kind of label-free fiber-optic biosensor probe based on enamel Fabry-Parot interferent |
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| CN109085140A (en) * | 2018-08-22 | 2018-12-25 | 东北大学 | A kind of high sensitivity optical fiber surface plasmon resonance biosensor |
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