CN208684818U - System is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile - Google Patents
System is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile Download PDFInfo
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- CN208684818U CN208684818U CN201821288633.7U CN201821288633U CN208684818U CN 208684818 U CN208684818 U CN 208684818U CN 201821288633 U CN201821288633 U CN 201821288633U CN 208684818 U CN208684818 U CN 208684818U
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Abstract
The utility model discloses a kind of Insect cellculture supernatant protein special steriles, and system is concentrated by ultrafiltration, including hollow fiber column, waste liquid bottle and liquid storage bottle, pressure gauge and pressure-regulating valve are provided on the outlet tube of hollow fiber column, inlet, liquid outlet and the waste liquid port of hollow fiber column are connected separately with the first thermoplastic tube, pump line, the liquid feeding end of outlet tube and sewer pipe liquid feeding end are connected separately with the second thermoplastic tube, and the first thermoplastic tube is linked together with corresponding second thermoplastic tube by tube sealing machine；Liquid level sensor is provided in liquid storage bottle, the signal output end of liquid level sensor is electrically connected with the signal input part of controller, and the control output end of controller is electrically connected with the control signal of peristaltic pump.It is realized and is seamlessly connected by tube sealing machine between each pipeline of the utility model, it is ensured that gnotobasis, air filter ensure the gnotobasis of waste liquid bottle and liquid storage bottle；Albumen stoste is in constantly circulation enrichment stage, improves thickening efficiency, realizes sterile thick purifying, the concentration of cells and supernatant albumen.
Description
Technical field
The utility model relates to experiment equipments, more particularly, to a kind of Insect cellculture supernatant protein special sterile ultrafiltration
Concentration systems.
Background technique
Shape virus-insect cell expressioning system can realize secreting, expressing, and the protein structure and native protein of expression are more
It is similar, but its expression quantity is lower.It usually requires when thus shape virus-insect cell expressioning system is used as diagnostic reagent to egg
White stoste is concentrated by ultrafiltration, to meet sample detection demand.Currently, generally being surpassed using hollow fiber column to albumen stoste
Filter concentration, the foreign protein of small molecule is flowed out by waste liquid port when concentration, and concentrate is flowed out by liquid outlet.Existing hollow fiber column filtering
System cannot be guaranteed the gnotobasis of protein concentrate, thus usually require in actual production to the albumen after concentration further into
Row disinfecting action, it is time-consuming and laborious, and there is the contaminated risk of albumen during the filtration process, the albumen stoste report is caused when serious
It is useless, it extends manufacture cycle.
Summary of the invention
The utility model provides that a kind of thickening efficiency is high and to be capable of the Insect cellculture supernatant protein of sterile concentration special
With sterile ultrafiltration concentration systems.
To achieve the above object, the utility model takes following technical proposals:
System, including doughnut is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile described in the utility model
Column, waste liquid bottle and liquid storage bottle are provided with peristaltic pump between the liquid storage bottle and the inlet of hollow fiber column, the peristaltic pump
Pump line outlet end is connected with the inlet of doughnut column bottom, its other end extends in liquid storage bottle, doughnut capital
The liquid outlet in portion is connected by outlet tube with liquid storage bottle, and waste liquid port is connected by sewer pipe with the waste liquid bottle, described
It is provided with pressure gauge and pressure-regulating valve on outlet tube, first upwardly extended is provided with and communicated on the bottle stopper of the liquid storage bottle and is led
Tracheae is provided with and communicated with the second gas-guide tube upwardly extended on the bottle stopper of the waste liquid bottle, and first gas-guide tube and second are led
Air filter is respectively arranged at the top of tracheae；Inlet, liquid outlet and the waste liquid port of the hollow fiber column are separately connected
There is the first thermoplastic tube, the outlet end of the pump line, the liquid feeding end of outlet tube and sewer pipe liquid feeding end are connected separately with and described
The second thermoplastic tube that one thermoplastic tube matches, first thermoplastic tube are linked as one by tube sealing machine with corresponding second thermoplastic tube
Body；Liquid level sensor is provided in the liquid storage bottle, the signal output end of the liquid level sensor and the signal of controller input
End electrical connection, the control output end of the controller is electrically connected with the control signal of the peristaltic pump.
The length of first thermoplastic tube and the second thermoplastic tube is 30-50cm.
The liquid feeding end of the pump line is connected with third thermoplastic tube, and the outlet end of the outlet tube is connected with the 4th thermoplastic tube,
The third thermoplastic tube and the 4th thermoplastic tube all extend in the liquid storage bottle.
The utility model has the advantage of assembling and convenient disassembly are quick, it is ensured that is concentrated, is reduced in gnotobasis
Protein concentrate contaminated probability.When concentration, is realized and be seamlessly connected by tube sealing machine between each pipeline, it is ensured that gnotobasis,
Air filter ensures the gnotobasis of waste liquid bottle and liquid storage bottle；Small molecule foreign protein flows into waste liquid bottle through sewer pipe by waste liquid port
Interior, concentrate flows into liquid storage bottle through liquid storage pipe, is then fed again into hollow fiber column and is concentrated through peristaltic pump, makes albumen
Stoste be in constantly circulation enrichment stage, improve thickening efficiency, realize cells and supernatant albumen it is sterile it is thick purifying, it is dense
Contracting.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Specific embodiment
As shown in Figure 1, system, packet is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile described in the utility model
Hollow fiber column 1(i.e. GE hollow fiber column is included, article No.: UFP-10-E-4A, aperture 10KD, internal diameter 1mm, surface area 0.042m2,
Be resistant to high pressure sterilization), waste liquid bottle 2 and liquid storage bottle 3, be provided with wriggling between the liquid storage bottle 3 and the inlet of hollow fiber column 1
Pump 4,5 outlet end of pump line of the peristaltic pump 4 is connected with the inlet of 1 bottom of hollow fiber column, its other end extends to storage
In liquid bottle 3, the liquid outlet at 1 top of hollow fiber column is connected by outlet tube 6 with liquid storage bottle 3, its waste liquid port passes through sewer pipe 7
It is connected with the waste liquid bottle 2, is provided with pressure gauge 8.1 and pressure-regulating valve 8.2 on the outlet tube 6, it is sterile convenient for adjusting
The pressure of the ultrafiltration circulatory system is provided with and communicated with the first gas-guide tube 9.1 upwardly extended on the bottle stopper of the liquid storage bottle 3, described
It is provided with and communicated with the second gas-guide tube 9.2 upwardly extended on the bottle stopper of waste liquid bottle 2, is provided at the top of first gas-guide tube 9.1
The top of air filter 10.1, the second gas-guide tube 9.2 is provided with air filter 10.2, it is ensured that waste liquid bottle 2 and liquid storage bottle 3
Gnotobasis；That length is 30~50cm is connected separately in the inlet of the hollow fiber column 1, liquid outlet and waste liquid port
One thermoplastic tube 11.1,11.2,11.3, the outlet end of the pump line 5 are connected with the inlet of the second thermoplastic tube 12.1, outlet tube 6
Be connected with the second thermoplastic tube 12.2,7 one end of sewer pipe is connected with the second thermoplastic tube 12.3, before concentration, by the first thermoplastic tube 11.1
With the second thermoplastic tube 12.1 by tube sealing machine seamless interfacing, the first thermoplastic tube 11.2 and the second thermoplastic tube 12.2 pass through tube sealing machine without
Seam docking, the first thermoplastic tube 11.3 and the second thermoplastic tube 12.3 realize the nothing of the entire circulatory system by tube sealing machine seamless interfacing
Bacterium connection；Liquid level sensor 13, the signal output end and controller 14 of the liquid level sensor 13 are provided in the liquid storage bottle 3
Signal input part electrical connection, the control output end of the controller 14 is electrically connected with the control signal of the peristaltic pump 4.
As shown in Figure 1, the liquid feeding end of the pump line 5 is connected with third thermoplastic tube 15, the liquid outlet end of the outlet tube 6 connects
It is connected to the 4th thermoplastic tube 16, the third thermoplastic tube 15 and the 4th thermoplastic tube 16 are each passed through the sealing-plug and extend to the storage
In liquid bottle 3, realize that sterile replacement is equipped with the liquid storage bottle 3 of different albumen stostes.Connection is spaced on the sealing-plug of liquid storage bottle 3 to be replaced
Setting there are two thermoplastic tube, when replacement by third thermoplastic tube 15 and the 4th thermoplastic tube 16 respectively with liquid storage bottle 3 to be replaced wherein
One thermoplastic tube is integrated by tube sealing machine seamless interfacing, and replacement is simple and convenient, while can ensure sterile replacement.
When concentration, sterilization treatment first is carried out to entire sterile ultrafiltration system, then the albumen stoste being concentrated will be needed in nothing
It is poured under the conditions of bacterium in liquid storage bottle 3, third heat-shrink tube and the 4th heat-shrink tube are inserted into respectively in liquid storage bottle 3 (or by third heat
The draw and the 4th thermoplastic tube 16 are linked together with the thermoplastic tube of liquid storage bottle 3 itself respectively), then by the first heat-shrink tube with it is corresponding
Second heat-shrink tube is connected as one by tube sealing machine, it is ensured that the gnotobasis of entire ultrafiltration system guarantees protein concentrate product
Gnotobasis is adjusted in the flow velocity (i.e. 200-400mL/min) and liquid storage bottle 3 of peristaltic pump 4 by the control panel of controller 14
Liquid level after the completion of concentration adjusts 6 pipeline pressure of outlet tube to 0.05-0.1Mpa by pressure-regulating valve 8.2, starts peristaltic pump
4 can carry out circulation concentration operation, and cyclic process small molecular foreign protein is flowed by the waste liquid port of hollow fiber column 1 through sewer pipe 7
Enter to waste liquid bottle 2, concentrate is flowed into liquid storage bottle 3 by concentration liquid outlet through outlet tube 6, is then again introduced into doughnut
Column 1 is concentrated；When liquid level reaches preset value in liquid storage bottle 3, to controller 14 signal, controller occur for liquid level sensor 13
14 issue the instruction to stop working to peristaltic pump 4, and concentration terminates.
Claims (3)
1. system, including hollow fiber column, waste liquid bottle and storage is concentrated by ultrafiltration in a kind of Insect cellculture supernatant protein special sterile
Liquid bottle, it is characterised in that: peristaltic pump, the pump of the peristaltic pump are provided between the liquid storage bottle and the inlet of hollow fiber column
Pipe outlet end is connected with the inlet of doughnut column bottom, its other end extends in liquid storage bottle, doughnut column top
Liquid outlet be connected by outlet tube with liquid storage bottle, waste liquid port is connected by sewer pipe with the waste liquid bottle, it is described go out
It is provided with pressure gauge and pressure-regulating valve in liquid pipe, the first air guide upwardly extended is provided with and communicated on the bottle stopper of the liquid storage bottle
It manages, is provided with and communicated with the second gas-guide tube upwardly extended, first gas-guide tube and the second air guide on the bottle stopper of the waste liquid bottle
Air filter is respectively arranged at the top of pipe；Inlet, liquid outlet and the waste liquid port of the hollow fiber column are connected separately with
First thermoplastic tube, the outlet end of the pump line, the liquid feeding end of outlet tube and sewer pipe liquid feeding end are connected separately with and described first
The second thermoplastic tube that thermoplastic tube matches, first thermoplastic tube are linked together with corresponding second thermoplastic tube by tube sealing machine；
Liquid level sensor, the signal output end of the liquid level sensor and the signal input part electricity of controller are provided in the liquid storage bottle
Connection, the control output end of the controller is electrically connected with the control signal of the peristaltic pump.
2. system is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile according to claim 1, it is characterised in that:
The length of first thermoplastic tube and the second thermoplastic tube is 30-50cm.
3. system is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile according to claim 1 or 2, feature exists
Third thermoplastic tube is connected in: the liquid feeding end of the pump line, the outlet end of the outlet tube is connected with the 4th thermoplastic tube, and described
Three thermoplastic tubes and the 4th thermoplastic tube all extend in the liquid storage bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821288633.7U CN208684818U (en) | 2018-08-10 | 2018-08-10 | System is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821288633.7U CN208684818U (en) | 2018-08-10 | 2018-08-10 | System is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208684818U true CN208684818U (en) | 2019-04-02 |
Family
ID=65884616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821288633.7U Active CN208684818U (en) | 2018-08-10 | 2018-08-10 | System is concentrated by ultrafiltration in Insect cellculture supernatant protein special sterile |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208684818U (en) |
-
2018
- 2018-08-10 CN CN201821288633.7U patent/CN208684818U/en active Active
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