CN208577730U - A kind of nucleic acid detection apparatus - Google Patents
A kind of nucleic acid detection apparatus Download PDFInfo
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- CN208577730U CN208577730U CN201821086679.0U CN201821086679U CN208577730U CN 208577730 U CN208577730 U CN 208577730U CN 201821086679 U CN201821086679 U CN 201821086679U CN 208577730 U CN208577730 U CN 208577730U
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Abstract
The utility model relates to a kind of nucleic acid detection apparatus, which includes sample processor and magnetosensitive detector；Sample processor includes reagent chamber, multiple PCR reaction fluid channel, capture chip memory room, DNA modification magnetic bead storage room and cleaning solution storage rooms being set side by side；The top of first PCR reaction fluid channel is connected to by the first microchannel with reagent exit, and two neighboring PCR reaction fluid channel is connected to by the second microchannel head and the tail；It is successively arranged 3 temperature controllers from top to bottom in each PCR reaction fluid channel；The import of capture chip memory room is connected to by third microchannel with the bottom end for reacting fluid channel last PCR, and is connected to by the 4th microchannel with DNA modification magnetic bead storage room, is connected to by the 5th microchannel with cleaning solution storage room；DNA modification magnetic bead in the Magnetic Sensor induction capture chip memory room of magnetosensitive detector, and electric signal is converted by the magnetic signal of DNA modification magnetic bead.Nucleic acid is detected using the device, reaction speed is fast and stable output signal.
Description
Technical field
The utility model relates to a kind of detection devices, and in particular to a kind of nucleic acid detection apparatus.
Background technique
Polymerase chain reaction (PCR) is a kind of specific for expanding based on DNA complementation and high temperature-resisting DNA polymerase
The Protocols in Molecular Biology of DNA fragmentation.Standard PCR instrument used at present, which has, to be possessed up to dozens of and can carry out accurate control
PCR reactive tank, this makes PCR instrument that can carry out the different PCR reaction of reaction condition simultaneously and in large quantity.However, for quickly,
It is to be time-consuming and expensive for simple DNA detection.Therefore, the PCR micro-analysis system based on microelectromechanical systems obtains
More and more applications.No. CN104745446A in PCR micro-analysis system disclosed in the patents such as No. CN202744555U,
PCR reaction mixture in fluid channel back and forth to pass through pre-set steady temperature area, so that it is anti-to quickly complete PCR
It answers.
However, the PCR micro-analysis system including above-mentioned patent publication still uses fluorescent dye and fluorescence signal point
Analysis system carries out detection analysis to the DNA amplified, and fluorescence signal has the characteristics that unstable and easy decaying, and its reagent
Condition of storage is also relatively harsh.
Utility model content
The purpose of the utility model is to overcome providing in place of above-mentioned the deficiencies in the prior art, a kind of reaction speed is fast, believes
Number stable nucleic acid detection apparatus of output.
To achieve the above object, a kind of technical solution that the utility model is taken are as follows: nucleic acid detection apparatus comprising sample
Processor and magnetosensitive detector；The sample processor includes reagent chamber, multiple PCR reaction fluid channels being set side by side, capture
Chip memory room, DNA modification magnetic bead storage room and cleaning solution storage room；
The reagent chamber is equipped with reagent entry port and reagent exit；The top of first PCR reaction fluid channel passes through the
One microchannel is connected to the reagent exit, and the two neighboring PCR reaction fluid channel is connected to by the second microchannel head and the tail, and
First microchannel is equipped with valve；It is successively arranged 3 temperature controllers from top to bottom in each PCR reaction fluid channel, point
Not Wei for controlling the first temperature controller of DNA denaturation temperature, the second temperature controller for controlling DNA annealing temperature, for controlling
The third temperature controller of DNA elongating temperature；
The import of the capture chip memory room is connected by third microchannel with the bottom end for reacting fluid channel the last PCR
It is logical, and be connected to by the 4th microchannel with the DNA modification magnetic bead storage room, it is stored by the 5th microchannel and the cleaning solution
Room connection；The third microchannel, the 4th microchannel and the 5th microchannel are equipped with for controlling PCR reactant, DNA modification magnetic
Pearl and cleaning solution separately flow into the valve of the capture chip memory room；
The magnetosensitive detector includes Magnetic Sensor and the groove that can accommodate capture chip memory room, the capture chip storage
Deposit the inside that room inserts in the groove, the DNA modification magnetic bead in the Magnetic Sensor induction capture chip memory room, and by DNA
The magnetic signal of modification magnetic bead is converted into electric signal.
Further, the reagent chamber is located at the top of PCR reaction fluid channel, and the capture chip memory room is located at
The lower section of the DNA modification magnetic bead storage room and cleaning solution storage room, and the height of the capture chip memory room is not higher than institute
State the bottom end of PCR reaction fluid channel.
Further, the nucleic acid detection apparatus further includes pressurizer, the pressurizer and the reagent chamber, DNA modification
Magnetic bead storage room and cleaning solution storage room are separately connected.
Further, the reagent chamber includes DNA accommodating chamber and PCR reaction solution accommodating chamber to be measured, and the DNA to be measured is accommodated
Reagent entry port and reagent exit are equipped on room and PCR reaction solution accommodating chamber；The DNA accommodating chamber to be measured is reacted with the PCR
The connection of liquid accommodating chamber；The reagent exit of the DNA accommodating chamber to be measured or PCR reaction solution accommodating chamber reacts micro- with the first PCR
The top of runner is connected to.
Further, the DNA accommodating chamber to be measured is located at the top of the PCR reaction solution accommodating chamber, and the DNA to be measured holds
The reagent exit of room received is connected to the reagent entry port of the PCR reaction solution accommodating chamber, and the reagent of the PCR reaction solution accommodating chamber goes out
Mouth reacts the top connection of fluid channel with first PCR.
Further, the reagent chamber further includes the extraction chamber DNA for being built-in with DNA extracting solution, the extraction chamber DNA and institute
State the reagent entry port connection of DNA accommodating chamber to be measured.
Further, the reagent chamber further includes the extraction chamber RNA and the reverse transcription reagents storage for being built-in with RNA extracting solution
Room, the reverse transcription reagents storage room and the reagent exit of the extraction chamber RNA and the reagent entry port of the DNA accommodating chamber to be measured
It is respectively communicated with.
Further, second microchannel reacts fluid channel integrated molding with multiple PCR.
Further, the PCR reaction fluid channel is 10~30.
Further, the temperature controller includes calandria, is electrically connected with the calandria and detects the temperature of heating temperature
Degree sensor and the temperature control unit that heating temperature is electrically connected and controlled with the calandria；The temperature sensor also with
Temperature control unit electrical connection, the temperature of the calandria for will test out are transferred to the temperature control unit；The heating
Body is set in PCR reaction fluid channel.
Further, the sample processor further includes waste liquid pool, the waste liquid pool and the capture chip memory room
Outlet, the pipeline for being connected to the outlet of the waste liquid pool and the capture chip memory room are equipped with valve.
Further, the valve is mechanical valve or solenoid valve.
Further, the mechanical valve is mechanical baffle or mechanical shutter；The solenoid valve is miniature electromagnetic valve.
In addition, the utility model additionally provides a kind of nucleic acid detection method comprising following steps:
(1) DNA to be measured and PCR reaction solution are mixed, mixed liquor is obtained；
(2) enter mixed liquor obtained by step (1) sequentially in multiple PCR reaction fluid channel, carry out multiple PCR reaction, obtain
To PCR reaction product；
(3) it reacts PCR reaction product obtained by step (2) with the capture chip containing capture dna, obtains containing to be measured
The capture chip of DNA；
(4) after having reacted, unbonded DNA is washed away with cleaning solution；
(5) the capture chip obtained by step (3) containing DNA to be measured is reacted with DNA modification magnetic bead；
(6) signal is detected by magnetosensitive detector.
Further, the nucleic acid detection method the following steps are included:
(1) DNA to be measured is placed in reagent chamber with PCR reaction solution and is mixed, obtain mixed liquor；
(2) it opens connection reagent chamber and reacts the valve on the first microchannel of fluid channel with first PCR, make step (1) institute
It obtains mixed liquor sequentially to enter in multiple PCR reaction fluid channel, carries out multiple PCR reaction, obtain PCR reaction product；
(3) valve being connected on the third microchannel of end PCR reaction fluid channel and capture chip memory room is opened, makes to walk
Suddenly PCR reaction product obtained by (2) is reacted with the capture chip containing capture dna, obtains the capture chip containing DNA to be measured；
(4) after having reacted, the valve on connection capture chip memory room and the 5th microchannel of cleaning solution storage room is opened,
Unbonded DNA is washed away with cleaning solution；
(5) valve on connection capture chip memory room and the 4th microchannel of DNA modification magnetic bead storage room is opened, makes to walk
Suddenly the capture chip obtained by (3) containing DNA to be measured is reacted with DNA modification magnetic bead；
(6) signal is detected by magnetosensitive detector.
Compared with prior art, the utility model has the following beneficial effects: the nucleic acid detection apparatus of the utility model is provided with
PCR reacts fluid channel, and PCR reaction carries out in fluid channel, and recurring number is controllable, and reaction speed is fast；The utility model is further adopted
With magnetosensitive detector, stable output signal, and will not fail at any time.
It can quantitative detection DNA marker using the nucleic acid detection apparatus of the utility model.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of sample processor in 1 nucleic acid detection apparatus of the utility model embodiment；
Fig. 2 is the structural schematic diagram of sample processor in 2 nucleic acid detection apparatus of the utility model embodiment.
In figure, 1 is reagent chamber, and 11 be DNA accommodating chamber to be measured, and 12 be PCR reaction solution accommodating chamber, and 13 be the extraction chamber DNA, 14
It is reverse transcription reagents storage room for the extraction chamber RNA, 15,2 react fluid channel for PCR, and 31 be the first temperature controller, and 32 be the second temperature control
Device, 33 be third temperature controller, 41 for capture chip memory room, 42 be DNA modification magnetic bead storage room, 43 be cleaning solution storage room, 5
It is waste liquid pool for pressurizer, 6,71 be the first microchannel, and 72 is micro- logical for second, and 73 be third microchannel, and 74 be the 4th microchannel,
75 be the 5th microchannel, and 81 be the first valve, and 82 be the second valve, and 83 be third valve, and 84 be the 4th valve, and 85 be the 5th valve
Door.
Specific embodiment
For the purpose of this utility model, technical solution and advantage is better described, below in conjunction with attached drawing and specific implementation
The utility model is described in further detail for example.
Embodiment 1
A kind of nucleic acid detection apparatus of the utility model embodiment comprising sample processor and magnetosensitive detector；This reality
The structure for applying a sample processor is as shown in Figure 1 comprising reagent chamber 1, multiple PCR reaction fluid channels 2 being set side by side, capture
Chip memory room 41, DNA modification magnetic bead storage room 42 and cleaning solution storage room 43；
Reagent chamber 1 is equipped with reagent entry port and reagent exit；The top of first PCR reaction fluid channel 2 is micro- logical by first
Road 71 is connected to the reagent exit of reagent chamber 1, and two neighboring PCR reaction fluid channel 2 is connected to from beginning to end by the second microchannel 72, this
The multiple PCR reaction fluid channels 2 of sample connect and form the flow path of detour, and the first microchannel 71 is equipped with the first valve 81；It is each
It is successively arranged 3 temperature controllers from top to bottom in PCR reaction fluid channel 2, respectively for controlling the first temperature control of DNA denaturation temperature
Device 31, the second temperature controller 32 for controlling DNA annealing temperature, the third temperature controller 33 for controlling DNA elongating temperature；
The import of capture chip memory room 41 is connected to by third microchannel 73 with the bottom end for reacting fluid channel 2 last PCR,
And be connected to by the 4th microchannel 74 with DNA modification magnetic bead storage room 42, pass through the 5th microchannel 75 and cleaning solution storage room 43
Connection；Third microchannel 73 is equipped with the second valve 82, and the 4th microchannel 74 is equipped with third valve 83, on the 5th microchannel 75
Equipped with the 5th valve 85, a valve in the second valve 82, third valve 83 and the 5th valve 85 is opened, it is ensured that PCR is anti-
Object, DNA modification magnetic bead and cleaning solution is answered to separately flow into capture chip memory room 41, i.e. guarantee PCR reactant, DNA modification magnetic bead
With cleaning solution independent incoming seizure chip memory room 41.
The magnetosensitive detector (structure of magnetosensitive detector is existing structure, is not drawn into figure) of the present embodiment includes that magnetic passes
Sensor and on magnetosensitive detector and can accommodate capture chip memory room groove, capture chip memory room 41 insert in groove
Inside, Magnetic Sensor induction captures the DNA modification magnetic bead in chip memory room 41, and the magnetic signal of DNA modification magnetic bead is converted
For electric signal.
The nucleic acid detection apparatus of the present embodiment is provided with multiple concatenated PCR and reacts fluid channel 2, and PCR reacts in fluid channel 2
Be used to make DNA that can be denaturalized, anneal and extend respectively equipped with multiple temperature controllers, in this way when DNA to be measured and PCR reaction solution enter it is each
When PCR reacts fluid channel 2, DNA can be denaturalized, anneals and extend, that is, a PCR occurs；When DNA to be measured and PCR reaction solution enter
When the second microchannel 72, reacted without PCR.The number that fluid channel 2 is reacted by setting PCR, can carry out multiple PCR and follow
Ring.Number, that is, PCR cycle number of PCR reaction fluid channel 2 can be set according to actual needs, it is preferable that PCR reacts fluid channel
2 be 10~30, such as 10,20,30 etc..Certainly, the number that PCR reacts fluid channel 2 is not limited to 10~30,
Less than 10 or 30 can be more than, 10~30 are a preferred amount.Fluid channel is reacted by setting PCR, micro-
PCR reaction carries out in runner, and recurring number is controllable, and reaction speed is fast.
After reaction product in PCR reaction fluid channel 2 enters capture chip memory room 41, can be captured chip memory room 41
In capture dna capture, then with connect DNA modification magnetic bead reaction, by detection magnetic signal can be detected DNA to be measured.Pass through inspection
Magnetic signal, stable output signal are surveyed, and will not be failed at any time.Detector is magnetosensitive detector, such as GMR detector.
It should be noted that the temperature of the first temperature controller 31 need to control within the scope of DNA denaturation temperature, it is denaturalized DNA,
Such as its temperature can be 90 DEG C；The temperature of second temperature controller 32 need to control in DNA annealing region, and DNA is made to anneal, such as
Its temperature can be 50 DEG C；The temperature of third temperature controller 33 need to control within the scope of DNA elongating temperature, extend DNA, such as its temperature
Degree can be 72 DEG C.Certainly, the denaturation, annealing of DNA, elongating temperature are all well known to those skilled in the art, this field
Technical staff can need to be adjusted and select according to Conventional wisdom and experiment.
Further, reagent chamber 1 is located at the top of PCR reaction fluid channel 2, and capture chip memory room 41 is located at DNA modification
The lower section of magnetic bead storage room 42 and cleaning solution storage room 43, and the height for capturing chip memory room 41 reacts miniflow not higher than PCR
The bottom end in road 2.According to specific each component of orientation, is conducive to reagent in this way and smoothly flows from top to bottom.Certainly,
Reagent can be made according to scheduled flow path by external force.
Further, nucleic acid detection apparatus further includes pressurizer 5, pressurizer 5 and reagent chamber 1, DNA modification magnetic bead storage room
42 and cleaning solution storage room 43 be separately connected.Pressurizer 5 can be reagent chamber 1, DNA modification magnetic bead storage room 42 and cleaning solution storage
Room 43 provides pressure, and as reagent flowing provides driving force.The built-in compressed air-driven of pressurizer 5, is also possible to machinery pressure
Power drive
Further, reagent chamber 1 includes DNA accommodating chamber 11 to be measured and PCR reaction solution accommodating chamber 12, DNA accommodating chamber to be measured
11 and PCR reaction solution accommodating chamber 12 on be equipped with reagent entry port and reagent exit；DNA accommodating chamber 11 to be measured holds with PCR reaction solution
Receive room 12 connection；The reagent exit of DNA accommodating chamber 11 or PCR reaction solution accommodating chamber 12 to be measured reacts fluid channel 2 with first PCR
Top connection.At this point, DNA to be measured and PCR reaction solution are placed in two accommodating chambers, as long as the two is mixed into PCR, reaction is micro-
It can be reacted in runner 2.Of course, it is possible to by the reagent of the reagent exit of DNA accommodating chamber 11 to be measured and PCR reaction solution accommodating chamber 12
Inlet communication, and the reagent exit of PCR reaction solution accommodating chamber 12 is reacted to the top connection of fluid channel 2 with first PCR；It can also be with
The reagent exit of PCR reaction solution accommodating chamber 12 is connected to the reagent entry port of DNA accommodating chamber 11 to be measured, and DNA to be measured is accommodated
The reagent exit of room 11 reacts the top connection of fluid channel 2 with first PCR.
Preferably, DNA accommodating chamber 11 to be measured is located at the top of PCR reaction solution accommodating chamber 12, the examination of DNA accommodating chamber 11 to be measured
Agent outlet is connected to the reagent entry port of PCR reaction solution accommodating chamber 12, the reagent exit of PCR reaction solution accommodating chamber 12 and first PCR
React the top connection of fluid channel 2.
Further, reagent chamber 1 further includes the extraction chamber DNA 13 for being built-in with DNA extracting solution, the extraction chamber DNA 13 with it is to be measured
The reagent entry port of DNA accommodating chamber 11 is connected to.This carry out DNA extraction process can directly in utility model device.
Further, the second microchannel 72 reacts the integrated molding of fluid channel 2 with multiple PCR.
Further, the first temperature controller 31, the second temperature controller 32, third temperature controller 33 include calandria and calandria
It is electrically connected and detects the temperature sensor of heating temperature and the temperature control of heating temperature is electrically connected and controlled with calandria
Unit；Temperature sensor is also electrically connected with temperature control unit, and the temperature of the calandria for will test out is transferred to temperature control
Unit processed；Calandria is set in PCR reaction fluid channel 2.Temperature controller commonly used in the art can be used in the device of the utility model, temperature
The specific structure of control device does not mark in Fig. 1.Wherein, calandria can be strip, and such a calandria can be simultaneously to more
A PCR reaction fluid channel 2 heats；Calandria can also be with sheet, and a calandria is for individually reacting fluid channel to a PCR
2 heating.
For convenience of cleaning solution or other waste liquids are collected, nucleic acid detection apparatus further includes waste liquid pool 6, waste liquid pool 6 and capture core
The pipeline of the outlet of piece storage room 41, the outlet of connection waste liquid pool 6 and capture chip memory room 41 is equipped with the 4th valve
84。
Further, valve is mechanical valve or solenoid valve.Further, mechanical valve is mechanical baffle or mechanical shutter；
Solenoid valve is miniature electromagnetic valve.
The application method of the present embodiment nucleic acid detection apparatus is (i.e. using the side of the present embodiment nucleic acid detection apparatus measurement nucleic acid
Method) are as follows:
(1) DNA is extracted in the extraction chamber DNA 13, obtains DNA to be measured；
(2) DNA to be measured that step (1) obtains enters DNA accommodating chamber 11 to be measured by the extraction chamber DNA 13；
(3) DNA to be measured is mixed in PCR reaction solution accommodating chamber 12 with PCR reaction solution, obtains mixed liquor；
(4) it opens connection reagent chamber 1 and reacts the first valve 81 on the first microchannel 71 of fluid channel 2 with first PCR, make
Mixed liquor obtained by step (3) sequentially enters in multiple PCR reaction fluid channel 2, carries out multiple PCR reaction, obtains PCR reaction and produces
Object；
(5) second on the third microchannel 73 for being connected to end PCR reaction fluid channel 2 and capture chip memory room 41 is opened
Valve 82 reacts PCR reaction product obtained by step (4) with the capture chip containing capture dna, obtains containing DNA's to be measured
Capture chip；
(6) it after having reacted, opens on connection capture chip memory room 41 and the 5th microchannel 75 of cleaning solution storage room 43
The 5th valve 85, unbonded DNA is washed away with cleaning solution；
(7) the on connection capture chip memory room 41 and the 4th microchannel 74 of DNA modification magnetic bead storage room 42 is opened
Three valves 83 react the capture chip obtained by step (5) containing DNA to be measured with DNA modification magnetic bead；
(8) signal is detected by magnetosensitive detector.
Embodiment 2
A kind of nucleic acid detection apparatus of the utility model embodiment, the difference with embodiment 1 is only in sample processor
Middle reagent chamber 1 is different, and the sample processor of the present embodiment nucleic acid detection apparatus is not as shown in Fig. 2, reagent chamber 1 includes being built-in with
The extraction chamber DNA 13 of DNA extracting solution, but the reagent chamber 1 of the present embodiment further includes the extraction chamber RNA 14 for being built-in with RNA extracting solution
With reverse transcription reagents storage room 15, the reagent of reverse transcription reagents storage room 15 and the extraction chamber RNA and DNA accommodating chamber 11 to be measured
Import is respectively communicated with.
RNA can extract using the nucleic acid detection apparatus of the present embodiment and carry out RNA measurement.The detection of nucleic acids of the present embodiment fills
The application method set is only step (1) different from embodiment 1, the nucleic acid detection apparatus of the present embodiment in use, step (1) are as follows:
RNA is extracted in the extraction chamber RNA 14, the RNA of extraction enters reverse transcription reagents storage room 15 by the extraction chamber RNA, carries out reverse transcription,
Obtain DNA to be measured.
Finally, it should be noted that above embodiments are only to illustrate the technical solution of the utility model rather than to this realities
With the limitation of novel protected range, although being explained in detail referring to preferred embodiment to the utility model, this field it is common
It will be appreciated by the skilled person that can be with the technical solution of the present invention is modified or equivalently replaced, without departing from this reality
With the spirit and scope of new technique scheme.
Claims (13)
1. a kind of nucleic acid detection apparatus, which is characterized in that including sample processor and magnetosensitive detector；The sample processor packet
Include reagent chamber, multiple PCR reaction fluid channel, capture chip memory room, DNA modification magnetic bead storage room and cleaning solutions being set side by side
Storage room；
The reagent chamber is equipped with reagent entry port and reagent exit；The top of the first PCR reaction fluid channel is micro- by first
Channel is connected to the reagent exit, and the two neighboring PCR reaction fluid channel is connected to by the second microchannel head and the tail, and described
First microchannel is equipped with valve；It is successively arranged 3 temperature controllers from top to bottom in each PCR reaction fluid channel, respectively
For controlling the first temperature controller of DNA denaturation temperature, the second temperature controller for controlling DNA annealing temperature, prolonging for controlling DNA
Stretch the third temperature controller of temperature；
The import of the capture chip memory room is connected to by third microchannel with the bottom end for reacting fluid channel the last PCR,
And be connected to by the 4th microchannel with the DNA modification magnetic bead storage room, pass through the 5th microchannel and the cleaning solution storage room
Connection；The third microchannel, the 4th microchannel and the 5th microchannel are equipped with for controlling PCR reactant, DNA modification magnetic bead
The valve of the capture chip memory room is separately flowed into cleaning solution；
The magnetosensitive detector includes Magnetic Sensor and the groove that can accommodate capture chip memory room, the capture chip memory room
Insert in the inside of the groove, the DNA modification magnetic bead in the Magnetic Sensor induction capture chip memory room, and by DNA modification
The magnetic signal of magnetic bead is converted into electric signal.
2. nucleic acid detection apparatus as described in claim 1, which is characterized in that the reagent chamber is located at PCR reaction miniflow
The top in road, the capture chip memory room are located at the lower section of the DNA modification magnetic bead storage room and cleaning solution storage room, and institute
State bottom end of the height not higher than PCR reaction fluid channel of capture chip memory room.
3. nucleic acid detection apparatus as described in claim 1, which is characterized in that the nucleic acid detection apparatus further includes pressurizer,
The pressurizer is separately connected with the reagent chamber, DNA modification magnetic bead storage room and cleaning solution storage room.
4. nucleic acid detection apparatus as described in claim 1, which is characterized in that the reagent chamber include DNA accommodating chamber to be measured and
PCR reaction solution accommodating chamber is equipped with reagent entry port and reagent exit on the DNA accommodating chamber to be measured and PCR reaction solution accommodating chamber；
The DNA accommodating chamber to be measured is connected to the PCR reaction solution accommodating chamber；The DNA accommodating chamber to be measured or PCR reaction solution accommodating chamber
Reagent exit reacted with the first PCR fluid channel top connection.
5. nucleic acid detection apparatus as claimed in claim 4, which is characterized in that it is anti-that the DNA accommodating chamber to be measured is located at the PCR
The top of liquid accommodating chamber is answered, the reagent exit of the DNA accommodating chamber to be measured and the reagent entry port of the PCR reaction solution accommodating chamber connect
Logical, the reagent exit of the PCR reaction solution accommodating chamber reacts the top connection of fluid channel with the first PCR.
6. nucleic acid detection apparatus as described in claim 4 or 5, which is characterized in that the reagent chamber further includes being built-in with DNA to mention
The extraction chamber DNA of liquid is taken, the extraction chamber DNA is connected to the reagent entry port of the DNA accommodating chamber to be measured.
7. nucleic acid detection apparatus as described in claim 4 or 5, which is characterized in that the reagent chamber further includes being built-in with RNA to mention
The extraction chamber RNA and the reverse transcription reagents storage room of liquid are taken, the reverse transcription reagents storage room and the reagent of the extraction chamber RNA go out
The reagent entry port of mouth and the DNA accommodating chamber to be measured is respectively communicated with.
8. nucleic acid detection apparatus as described in claim 1, which is characterized in that second microchannel and multiple PCR are anti-
Fluid channel is answered to be integrally formed.
9. nucleic acid detection apparatus as described in claim 1, which is characterized in that the PCR reaction fluid channel is 10~30.
10. nucleic acid detection apparatus as described in claim 1, which is characterized in that the temperature controller include calandria, with it is described plus
Hot body is electrically connected and detects the temperature sensor of heating temperature and is electrically connected with the calandria and controls heating temperature
Temperature control unit；The temperature sensor is also electrically connected with temperature control unit, the temperature of the calandria for will test out
It is transferred to the temperature control unit；The calandria is set in PCR reaction fluid channel.
11. nucleic acid detection apparatus as described in claim 1, which is characterized in that the sample processor further includes waste liquid pool, institute
The outlet for stating waste liquid pool and the capture chip memory room is connected to the waste liquid pool with described and captures going out for chip memory room
The pipeline of mouth is equipped with valve.
12. the nucleic acid detection apparatus as described in claim 1 or 11, which is characterized in that the valve is mechanical valve or solenoid valve.
13. nucleic acid detection apparatus as claimed in claim 12, which is characterized in that the mechanical valve is mechanical baffle or mechanical gear
Plate；The solenoid valve is miniature electromagnetic valve.
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| CN201821086679.0U CN208577730U (en) | 2018-07-10 | 2018-07-10 | A kind of nucleic acid detection apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115190909A (en) * | 2020-03-31 | 2022-10-14 | Tdk株式会社 | Magnetic signal detection chip, detection card, nucleic acid detection device, and method for detecting composition containing target DNA fragment in chemical fluid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115190909A (en) * | 2020-03-31 | 2022-10-14 | Tdk株式会社 | Magnetic signal detection chip, detection card, nucleic acid detection device, and method for detecting composition containing target DNA fragment in chemical fluid |
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