CN109337807A - PCR assembly line - Google Patents
PCR assembly line Download PDFInfo
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- CN109337807A CN109337807A CN201811162664.2A CN201811162664A CN109337807A CN 109337807 A CN109337807 A CN 109337807A CN 201811162664 A CN201811162664 A CN 201811162664A CN 109337807 A CN109337807 A CN 109337807A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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Abstract
The invention discloses a kind of PCR assembly lines, belong to medical technical field of automation equipment, including nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis unit；The nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis unit are sequentially connected, and are that can close setting, and movement of the reagent between each unit is realized by mobile device；PCR assembly line of the invention, it is sequentially connected nucleic acid extraction unit, sample-adding unit, PCR reaction member and the data analysis unit of setting, each unit is only absorbed in the work for handling some segment, and by the production of automation, can be improved working efficiency and increase the flux for carrying out PCR reaction；Nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis unit are closed setting, therefore can be avoided the Aerosol Pollution between each unit；Movement of the reagent between each unit is realized by mobile device, therefore can be avoided the biohazard to operator.
Description
Technical field
The invention belongs to medical technical field of automation equipment, and in particular to a kind of PCR assembly line.
Background technique
Polymerase chain reaction (Polymerase Chain Reaction, PCR) is external enzyme' s catalysis specific DNA piece
A kind of method of section is one of most common Protocols in Molecular Biology.By high-temperature denaturation, low-temperature annealing (renaturation) and appropriate temperature extension
Equal reactions form a cycle, and circulation carries out, and rapidly amplifies target DNA, have high specificity, high sensitivity, operation
The features such as easy, time saving.
Carrying out PCR experiment at present is usually to be carried out by PCR Lab, and common PCR Lab can be divided into Si great Qu
Domain, preparation of reagents area, sample treatment area, nucleic acid amplification area, product analysis area.Centrifuge, ice can be placed in this four regions
The equipment and instruments such as case, heating unit, water bath, PCR instrument, electrophoresis apparatus.
However, between each region carry out reaction tube it is mobile when, be all manually to be moved by operator at present
Dynamic, the containers such as reaction tube generate biggish vibration in moving process, to form aerosol, and sample is nucleic acid-templated, micro- life
Object or virus all with aerosol or can form aerosol and spread, and operator is made to bear higher biohazard risk,
In addition, manually-operated efficiency is relatively low.
Summary of the invention
Therefore, the technical problem to be solved in the present invention is that needing manually to transfer reaction tube during existing PCR experiment,
Operator is set to bear higher biohazard risk, and the problem of inefficient operation, to provide a kind of PCR flowing water
Line.
PCR assembly line provided by the invention, including nucleic acid extraction unit have instrument for extracting nucleic acid, for carrying out mentioning for DNA
It takes；
It is loaded unit, for DNA extract to be added in PCR reaction reagent, forms reaction solution；
PCR reaction member has PCR instrument, for carrying out PCR reaction to the reaction solution after sample-adding；
Data analysis unit, for carrying out data analysis to PCR reactant；
The nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis unit are sequentially connected, and are
Setting can be closed, movement of the reagent between each unit is realized by mobile device.
Preferably, in sequentially connected nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis
It has been respectively communicated with isolation chamber between unit, has been provided with the mobile device in the separate cavities room.
Preferably, the mobile device by be located at each unit in reagent be moved to isolation chamber after, it is described every
From chamber by the displacement of inner air, the Aerosol Pollution between each reagent sequentially entered is prevented.
Preferably, further include,
Computer control module, for analyzing the nucleic acid extraction unit, sample-adding unit, PCR reaction member and data
Unit is attached and controls respectively.
Preferably, it is set in the nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis unit
There is data port, for being electrically connected with the computer control module.
Preferably, further include,
Data scanner, setting are single in the nucleic acid extraction unit, sample-adding unit, PCR reaction member or data analysis
In member, for scanning the identification code being located on reagent bottle, and data information is sent to the number in the computer control module
According to recognition unit.
Preferably, there is preset multiple groups PCR reaction reagent in the sample-adding unit, react and try in the PCR
There is preset identification code on the bottle of agent, after forming reaction solution, the PCR is reacted by the computer control module and is tried
Identification code on the bottle of agent is corresponding with the identification code on the bottle of the DNA extract.
Preferably, the PCR instrument is opened the door using side and is arranged.
Preferably, the PCR instrument in the PCR reaction member has multiple reaction chambers, each reaction chamber
Indoor reaction condition is able to carry out independent control by the computer control module.
Preferably, further include,
Storage unit has refrigerating equipment, for depositing to the residual reaction liquid for not carrying out PCR reaction in sample-adding unit
Storage, using as spare when being rechecked.
Preferably, further include,
Sample mixing unit is arranged between the nucleic acid extraction unit and the sample-adding unit, is used for multiple groups DNA extract
Mixing constitutes one group of DNA extracting solution.
Preferably, the data analysis of the data analysis unit includes being carried out by sequenator to PCR reactant
DNA sequencing.
Technical solution provided by the invention, has the advantages that
1, PCR assembly line provided by the invention, the nucleic acid extraction unit, sample-adding unit, PCR reaction for being sequentially connected setting are single
Member and data analysis unit, each unit is only absorbed in the work for handling some segment, and passes through the production of automation, energy
It enough improves working efficiency and increases the flux for carrying out PCR reaction；Nucleic acid extraction unit, sample-adding unit, PCR reaction member and data
Analytical unit is closed setting, therefore can be avoided the Aerosol Pollution between each unit；Reagent is between each unit
Movement realized by mobile device, therefore can be avoided the biohazard to operator.
2, PCR assembly line provided by the invention, in nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis
An isolation chamber is respectively communicated between unit, mobile device is arranged in separate cavities room, thereby it is ensured that moving passing through
When dynamic device moves reagent between each unit, aerosol diffusion is not had to the external world, to effectively prevent Aerosol Pollution
Diffusion, avoid in extraneous operator by biohazard；And the movement of reagent, Neng Gouti are carried out using mobile device
High operating efficiency further increases the flux for carrying out PCR reaction.
3, the air of PCR assembly line provided by the invention, the isolation chamber interior can be replaced as needed, from
And the cross contamination of the aerosol generated in each cell is avoided, guarantee the accuracy of experiment.
4, PCR assembly line provided by the invention has computer control module, can be distinguished by computer control module
The nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis unit are individually controlled, it can also be special by setting
Determining program makes above-mentioned each unit automatic operating, it is achieved that the automatic operation of assembly line, improves operating efficiency, further
Increase the flux for carrying out PCR reaction.
5, PCR assembly line provided by the invention, in nucleic acid extraction unit, sample-adding unit, PCR reaction member and data analysis
It is equipped with data port in unit, for being electrically connected with the computer control module, above-mentioned each unit is made to can be realized and calculate
The data of machine control module are transmitted.
6, PCR assembly line provided by the invention, have data scanner, setting nucleic acid extraction unit, sample-adding unit,
In PCR reaction member or data analysis unit, for scanning the identification code being located on reagent bottle, and data information is sent to meter
The reagent conveyed in each unit can be generated connection by data identification unit by the data identification unit in calculation machine control module
System can obtain sample information corresponding with testing result and Testing index information, be conducive to reality by reading information of identification code
The Auto-matching of existing PCR testing result and sample, indication information, and then realize that the automation of PCR test experience carries out；Meanwhile
In the case where pattern detection amount is big, by the differentiation of unique identifier on PCR reaction tube, it can be avoided detection information and beaten
Disorderly, the sample information situation not corresponding with testing result reduces the error rate of testing result.
7, PCR assembly line provided by the invention, sample-adding unit in have preset multiple groups PCR reaction reagent, pass through by
DNA extract is incorporated into sample mixing in each group PCR reaction reagent and the reaction solution is made, due to reducing to PCR reaction reagent
The time is prepared, the efficiency that reaction solution is made in sample mixing is improved, therefore can further increase the flux for carrying out PCR reaction；?
On the bottle of above-mentioned preset PCR reaction reagent there is preset identification code to control by computer, root after forming reaction solution
PCR reaction is carried out according to identification code sequence, is enabled a computer to convenient for being recorded to the reagent for carrying out PCR reaction, from artificial
Operation improves working efficiency.
8, PCR assembly line provided by the invention, the PCR instrument in PCR reaction member is opened the door using side to be arranged, compared to existing
There is the top opening of technology to be arranged, more reaction reagents can be accommodated, therefore the PCR that can once carry out more reaction solutions is anti-
It answers, increases the flux for carrying out PCR reaction.
9, PCR assembly line provided by the invention, the PCR instrument in PCR reaction member has multiple reaction chambers, each anti-
It, can be simultaneously more when answering the indoor reaction condition of chamber individually to be controlled by computer, therefore being reacted using PCR instrument
The reaction that multiple groups different condition is carried out in a reaction chamber, improves the efficiency for carrying out PCR experiment, further increases and carries out PCR
The flux of reaction.
10, PCR assembly line provided by the invention has storage unit, in sample-adding unit reaction solution made of sample mixing into
Row PCR only needs small part when reacting, and the residual reaction liquid for not carrying out PCR reaction can be by the refrigeration in storage unit
Equipment is stored, using as spare when being rechecked.
11, PCR assembly line provided by the invention has sample mixing unit, mixes multiple groups DNA extract in sample mixing unit
One group of DNA extracting solution is constituted, above-mentioned one group of DNA extracting solution is then added to PCR reaction reagent in subsequent sample-adding unit
Middle formation reaction solution carries out PCR reaction to above-mentioned reaction solution in subsequent PCR reaction member, can complete more to containing
The PCR reaction of the reaction solution of group DNA extract, it is disposable to complete if reaction result shows that DNA is no pathogenic infection
The detection of the above multiple groups DNA, therefore can be improved PCR reaction efficiency, increase the flux for carrying out PCR reaction.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the main view of PCR assembly line provided by the invention.
Description of symbols:
1, nucleic acid extraction unit；2, it is loaded unit；3, PCR reaction member；4, data analysis unit；5, mobile device；6,
Chamber is isolated；7, storage unit.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " on ", "lower", "left", "right", "vertical", "horizontal",
The orientation or positional relationship of the instructions such as "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of retouching
It states the present invention and simplifies description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with specific
Orientation construction and operation, therefore be not considered as limiting the invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
As long as in addition, the non-structure each other of technical characteristic involved in invention described below different embodiments
It can be combined with each other at conflict.
Embodiment 1
The present embodiment provides a kind of PCR assembly lines, as shown in Figure 1, anti-including nucleic acid extraction unit 1, sample-adding unit 2, PCR
Answer unit 3, data analysis unit 4 and storage unit 7.
The nucleic acid extraction unit 1, sample-adding unit 2, PCR reaction member 3 and data analysis unit 4 are sequentially connected, and
It is that can close setting, movement of the reagent between each unit is realized by mobile device 5.
Specifically, there is instrument for extracting nucleic acid, for carrying out the extraction of DNA inside the nucleic acid extraction unit 1；The sample-adding
Unit 2 forms reaction solution for DNA extract to be added in PCR reaction reagent；Have inside the PCR reaction member 3
PCR instrument, for carrying out PCR reaction to the reaction solution after sample-adding；Inside the data analysis unit 4 have sequenator, for pair
PCR reactant carries out DNA sequencing；The function of the reaction and realization that carry out in above-mentioned each unit is the prior art, is not done herein
It repeats, above-mentioned each unit is formed assembly line by the application, by the nucleic acid extraction unit 1, sample-adding unit 2, PCR reaction member 3
It is sequentially connected with data analysis unit 4 by the way that chamber 6 is isolated, is provided with shifting in the isolation chamber 6 being connected between each unit
Dynamic device 5 is carried out the reagent in each unit is located at into and out the isolation chamber 6 by the mobile device 5, thus
Realization transmits reagent between each reaction member.
Specifically, the mobile device 5 can use manipulator, conveyer belt or other existing robotic movement devices.
There is refrigerating equipment in storage unit 7, for the residual reaction liquid for not carrying out PCR reaction in sample-adding unit 2
It is stored, using as spare when being rechecked.
In order to prevent between each reaction member aerosol pollution, the isolation chamber 6 is provided with air displacer,
It is replaced for the inner air to isolation chamber 6, specifically, air displacer can use existing exhaust fan or pumping
Wind apparatus.
In order to improve the automation of pipelining, the nucleic acid extraction unit 1, sample-adding unit 2, PCR reaction member 3,
Data analysis unit 4, mobile device 5 and isolation chamber 6 are separately connected and are controlled by computer control module.
Data identification unit is equipped in computer control module, the data identification unit is mentioned for receiving positioned at nucleic acid
Take unit 1, sample-adding unit 2, in PCR reaction member 3 and data analysis unit 4 data scanner to identification code on reagent bottle
Scanning information, sample, the reaction solution or anti-that can will be transmitted in by the scanning to identification code on reagent bottle in a reaction member
Product is answered to be corresponded.
The PCR instrument is opened the door using side to be arranged, and the PCR instrument of the PCR reaction member 3 has multiple reaction chambers,
Each indoor reaction condition of the reaction chamber is individually controlled by computer.
There is preset multiple groups PCR reaction reagent in the sample-adding unit 2, by the way that DNA extract is incorporated into each group PCR
The reaction solution is made in sample mixing in reaction reagent.
It is provided with sample mixing unit between the nucleic acid extraction unit and the sample-adding unit, by multiple groups in sample mixing unit
The mixing of DNA extract constitutes one group of DNA extracting solution, and then above-mentioned one group of DNA extracting solution is added in subsequent sample-adding unit
Reaction solution is formed into PCR reaction reagent, PCR reaction is carried out to above-mentioned reaction solution in subsequent PCR reaction member, it can be complete
The PCR reaction of the reaction solution of multiple groups DNA extract is contained in pairs, if reaction result shows that DNA is no pathogenic infection,
The detection of the above multiple groups DNA is then disposably completed, therefore can be improved PCR reaction efficiency, increases and carries out the logical of PCR reaction
Amount.
The course of work:
When carrying out PCR experiment by the PCR assembly line of the application, successively the following steps are included:
In nucleic acid extraction unit, reagent to be reacted is subjected to DNA extraction.
DNA extract is moved to sample-adding unit by mobile device.
In sample-adding unit, DNA extract is added in PCR reaction reagent, forms reaction solution；The reaction solution part
It is partially spare for storing for carrying out PCR reaction.
It will be loaded in unit by mobile device and be used to store spare part reaction solution and be moved to storage unit, stored
It carries out refrigerating in unit spare.
The part reaction solution for being used to carry out PCR reaction in sample-adding unit is moved to PCR reaction member by mobile device.
PCR reaction is carried out to reaction solution in PCR reaction member.
The sample after reaction is moved to data analysis unit by mobile device.
DNA sequencing is carried out to sample in data analysis unit.
In above-mentioned reaction step, the Aerosol Pollution between each unit in order to prevent, each time by reagent in each unit
Between movement, first pass through isolation chamber, and air displacement at least once is carried out in separate cavities room.
In above-mentioned reaction step, in order to improve conventional efficient, increase the flux for carrying out PCR reaction, in nucleic acid extraction list
It is provided with sample mixing unit between member and sample-adding unit, multiple groups DNA extract is mixed in sample mixing unit and constitutes one group of DNA extraction
Liquid；In this way, forming one by the way that disposably multiple groups DNA extract is imported into same PCR reaction reagent in sample-adding unit
Reaction solution；Then in PCR reaction member, a PCR reaction is carried out to above-mentioned reaction solution, is if reaction result shows DNA
There is no pathogenic infection, then can disposably complete the detection of the above multiple groups DNA, if reaction result shows that DNA there are pathogenic bacteria
Infection, then the reaction solution with independent DNA extract is made in sample-adding again, carries out secondary PCR detection one by one.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (12)
1.PCR assembly line, comprising:
Nucleic acid extraction unit (1) has instrument for extracting nucleic acid, for carrying out the extraction of DNA；
It is loaded unit (2), for DNA extract to be added in PCR reaction reagent, forms reaction solution；
PCR reaction member (3) has PCR instrument, for carrying out PCR reaction to the reaction solution after sample-adding；
Data analysis unit (4), for carrying out data analysis to PCR reactant；
It is characterized in that, the nucleic acid extraction unit (1), sample-adding unit (2), PCR reaction member (3) and data analysis unit
(4) it is sequentially connected, and is that can close setting, movement of the reagent between each unit is realized by mobile device (5).
2. PCR assembly line according to claim 1, which is characterized in that in sequentially connected nucleic acid extraction unit (1), add
Isolation chamber (6), the separate cavities have been respectively communicated between sample unit (2), PCR reaction member (3) and data analysis unit (4)
The mobile device (5) are provided in room (6).
3. PCR assembly line according to claim 2, which is characterized in that the mobile device (5) will be located in each unit
After reagent is moved to isolation chamber (6), the described isolation displacement of chamber (6) by inner air prevents each examination sequentially entered
Aerosol Pollution between agent.
4. PCR assembly line according to claim 1, which is characterized in that further include,
Computer control module, for the nucleic acid extraction unit (1), sample-adding unit (2), PCR reaction member (3) and data
Analytical unit (4) is attached and controls respectively.
5. PCR assembly line according to claim 4, which is characterized in that the nucleic acid extraction unit (1), sample-adding unit
(2), it is equipped with data port in PCR reaction member (3) and data analysis unit (4), for electric with the computer control module
Connection.
6. PCR assembly line according to claim 5, which is characterized in that further include,
Data scanner, setting is in the nucleic acid extraction unit (1), sample-adding unit (2), PCR reaction member (3) or data point
It analyses in unit (4), for scanning the identification code being located on reagent bottle, and data information is sent to the computer control module
Interior data identification unit.
7. PCR assembly line according to claim 6, which is characterized in that
There is preset multiple groups PCR reaction reagent in the sample-adding unit (2), have on the bottle of the PCR reaction reagent pre-
If identification code, after forming reaction solution, by the computer control module by the identification on the bottle of the PCR reaction reagent
Code is corresponding with the identification code on the bottle of the DNA extract.
8. PCR assembly line according to claim 4, which is characterized in that the PCR instrument is opened the door using side to be arranged.
9. PCR assembly line according to claim 8, which is characterized in that the PCR instrument in the PCR reaction member (3) has
Multiple reaction chambers, each indoor reaction condition of reaction chamber are able to carry out independent control by the computer control module
System.
10. PCR assembly line according to claim 1, which is characterized in that further include,
Storage unit (7) has refrigerating equipment, for carrying out to the residual reaction liquid for not carrying out PCR reaction in sample-adding unit (2)
Storage, using as spare when being rechecked.
11. PCR assembly line according to claim 1, which is characterized in that further include,
Sample mixing unit is arranged between the nucleic acid extraction unit (1) and the sample-adding unit (2), for extracting multiple groups DNA
Object mixing constitutes one group of DNA extracting solution.
12. PCR assembly line according to claim 1, which is characterized in that the data of the data analysis unit (4) are analyzed
Including carrying out DNA sequencing to PCR reactant by sequenator.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811162664.2A CN109337807A (en) | 2018-09-30 | 2018-09-30 | PCR assembly line |
| PCT/CN2018/109326 WO2020062315A1 (en) | 2018-09-30 | 2018-10-08 | Pcr pipeline |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811162664.2A CN109337807A (en) | 2018-09-30 | 2018-09-30 | PCR assembly line |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109337807A true CN109337807A (en) | 2019-02-15 |
Family
ID=65308504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811162664.2A Pending CN109337807A (en) | 2018-09-30 | 2018-09-30 | PCR assembly line |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109337807A (en) |
| WO (1) | WO2020062315A1 (en) |
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| CN111575171A (en) * | 2020-07-03 | 2020-08-25 | 上海宏滩生物科技有限公司 | PCR workstation |
| WO2021219067A1 (en) * | 2020-04-30 | 2021-11-04 | 杭州杰毅生物技术有限公司 | Nucleic acid test pipeline processing apparatus, and pipeline molecular diagnostics apparatus |
| CN114369525A (en) * | 2020-10-19 | 2022-04-19 | 成都瀚辰光翼生物工程有限公司 | Gene detecting apparatus |
| CN117660175A (en) * | 2024-02-01 | 2024-03-08 | 上海千麦博米乐医学检验所有限公司 | PCR laboratory system for gene sample processing detection |
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Also Published As
| Publication number | Publication date |
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| WO2020062315A1 (en) | 2020-04-02 |
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