CN208520676U - A kind of rapid tissue freezing pretreatment unit - Google Patents
A kind of rapid tissue freezing pretreatment unit Download PDFInfo
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- CN208520676U CN208520676U CN201821302778.8U CN201821302778U CN208520676U CN 208520676 U CN208520676 U CN 208520676U CN 201821302778 U CN201821302778 U CN 201821302778U CN 208520676 U CN208520676 U CN 208520676U
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- China
- Prior art keywords
- cup body
- frozen section
- prop head
- sleeve
- cylinder
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Abstract
The utility model relates to a kind of rapid tissues to freeze pretreatment unit, belong to experimental provision field.The rapid tissue freezing pretreatment unit includes: cylindrical cup body, and cup lid is arranged on the cylinder cup body, and the cup lid is detachably connected with the cylindrical cup body sealing, and cup lid handle is arranged in the cup lid centre；Sleeve is welded at the cylinder cup body bottom centre position, the sleeve is hollow circuit cylinder, and for inserting frozen section prop head, the frozen section prop head is made of the sleeve frozen section prop head disc-shaped structure and frozen section prop head cylindrical structure；The outside mirror image of the cylinder cup body opening portion is symmetrical arranged two quarter butts, the outside welding of one end of the quarter butt and the cylindrical cup body opening portion, and the other end of the quarter butt is welded with annulus.Isopentane in the device is in the case where not contacting liquid nitrogen by Liquid nitrogen precooler to desired temperature, and the device can be avoided isopentane and quickly volatilize, and realizes that flesh tissue is uniformly cooled.
Description
Technical field
The utility model relates to a kind of rapid tissues to freeze pretreatment unit, belong to experimental provision field.
Background technique
Frozen section be it is a kind of make tissue be cooled fast to certain degree of hardness under cryogenic to be sliced, then be immunized
The histology means of fluorescent staining or immunohistochemical staining observation, are a kind of widely used histological techniques.It is freezing
During slice, most important problem is that tissue pre-cooling is uneven, and ice crystal crack occurs in tissue space, and institutional framework is caused to damage
Evil, influences to observe.Especially for heart, liver, brain, for the solid tissues such as kidney, since its tissue is thicker, rapid cooling group
Difficulty is knitted, the quality of frozen section is improved, shortens time of frozen section for histological observation and pathological diagnosis to closing weight
It wants, this just puts forward higher requirement to tissue freezing pretreatment.
The research of the past has carried out multiple improvement for Refrigeration Technique, freezing tissue mode general at present are as follows: first will
Fresh tissue specimen is placed in conventional freezing microtome prop head, with OCT (Optimal Cutting Temperature) packet
Agent embedding infiltration tissue is buried, which is the water soluble mixt of a kind of polyethylene glycol and polyvinyl alcohol, packet
The purpose buried is supporting tissue, reduces fold and fragmentation.Then isopentane is taken with beaker Sheng, is pre-chilled 5-10 minutes in liquid nitrogen.
Wherein the temperature of liquid nitrogen is -196 DEG C, and the melting temperature of isopentane is -159.4 DEG C, directly violent with liquid nitrogen frozen tissue liquid nitrogen
Volatilization, be easy to cause in tissue and forms crack, can play preferable freezing effect as medium using the isopentane of liquid low temperature
Fruit.It will finally be loaded with and immersed in the isopentane being pre-chilled with the prop head of the embedded tissue block of OCT glue, freezed 1 minute or so,
Sectioning can be carried out later.
Pretreated whole process is freezed before still frozen section can be completed without dedicated device at present, the prior art is deposited
The problem of be: (1) since liquid nitrogen temperature is low compared with isopentane fusing point, the beaker for being contained with isopentane, which is directly immersed in liquid nitrogen, to lead
Isopentane crystallization is caused not to be available for solid-state；(2) frozen section prop head does not carry during immersing the isopentane being pre-chilled
Body is easy to cause prop head crooked, and tissue is cooled unevenly, ice crystal and crack occurs, damages tissue morphology；(3) isopentane is normal
Temperature is readily volatilized, does harm to huamn body.
Utility model content
The purpose of this utility model is to provide a kind of rapid tissues to freeze pretreatment unit, and it is cold to solve medical research field
Isopentane sufficiently cannot effectively be pre-chilled during jelly, and tissue block is cooled non-uniform technical problem.
The utility model provides the following technical solutions: a kind of rapid tissue freezing pretreatment unit, comprising:
Cylindrical cup body, the cylinder cup body is for holding isopentane；
Cup lid is set on the cylinder cup body, and the cup lid is detachably connected with the cylindrical cup body sealing, is used for
The isopentane is closed in the cylindrical cup body；
Cup lid handle is arranged in the cup lid centre, for lifting the cup lid；
Sleeve is welded at the cylinder cup body bottom centre position, the sleeve is hollow circuit cylinder, and the sleeve is used
In inserting frozen section prop head, the frozen section prop head is by frozen section prop head disc-shaped structure and frozen section prop head cylinder
Structure composition, the frozen section prop head is for placing embedded flesh tissue；
The outside mirror image of the cylinder cup body opening portion is symmetrical arranged two quarter butts, one end of the quarter butt and the circle
The outside of cylindrical cup opening portion is welded, and the other end of the quarter butt is welded with annulus, is to have nylon rope on the annulus, is used for
The cylindrical cup body is suspended to liquid nitrogen container inner close fitting liquid nitrogen ullage.
Embodiment according to the present utility model, the utility model is to one of the main contributions of the prior art, using nylon
Rapid tissue freezing pretreatment unit is suspended to liquid nitrogen container inner close fitting in liquid nitrogen ullage by rope, keeps cylindrical cup intracorporal different
Pentane is not in the case where contacting liquid nitrogen by Liquid nitrogen precooler to desired temperature；Meanwhile frozen section prop head can be smoothly inserted in
In sleeve, embedded flesh tissue can be made reliably to place, embedded flesh tissue is by the isopentane in cylindrical cup body
It can be uniformly cooled after submergence, be not in ice crystal and slight crack, form is intact after embedded flesh tissue is cooled；Finally,
The sealed connection of cup lid and the cylindrical cup body prevents isopentane from volatilizing, and avoids harm to the human body.
In optional technical solution, the cylinder cup body, the cup lid, cup lid handle, the sleeve, the quarter butt
Stainless steel material is all made of with the annulus to be made, and effectively improves the service life of rapid tissue freezing pretreatment unit.
In optional technical solution, it is highly 70-90mm that the diameter of the cylinder cup body, which is 40-60mm,；The sleeve
Diameter be 9-11mm, a height of 20-30mm；The diameter ratio of the cylinder cup body and the sleeve is 6.6-3.5, the cylinder
The height ratio of shape cup body and the sleeve is 4.5-2.3.
In optional technical solution, it is highly 20-25mm that the frozen section prop head cylindrical structure diameter, which is 5-8mm, institute
State frozen section prop head disc-shaped structure diameter be 25-30mm, be highly 4-6mm, the frozen section prop head cylindrical structure with
The centre bore of the sleeve matches, and realizes steadily inserting for the frozen section prop head.
Embodiment according to the present utility model, the cylinder cup body, frozen section prop head disc-shaped structure and described
The various structures of the components such as sleeve are sized to meet the uniform pre-cooling of various sizes of embedded flesh tissue, with auxiliary
Completion is helped to be sliced.
In optional technical solution, the cup lid is that seal with elastometic washer is connect with the sealed connection of the cylindrical cup body.
The good effect of the utility model:
(1) after having held isopentane in rapid tissue described in the utility model freezing pretreatment unit, cup lid is covered,
The present apparatus is suspended to ullage 10 minutes by liquid nitrogen container inner close fitting liquid nitrogen using nylon rope, isopentane is made not contact liquid nitrogen
In the case of by Liquid nitrogen precooler to desired temperature.
(2) sleeve in rapid tissue described in the utility model freezing pretreatment unit can directly will be embedded fresh
The slicer prop head of tissue steadily inserts, and the isopentane after flesh tissue is pre-chilled sufficiently all submerges, and is uniformly cooled.
(3) cup lid in rapid tissue freezing pretreatment unit described in the utility model and cylindrical cup body are that sealing can
Isopentane can be closed in cylindrical cup body, isopentane is avoided quickly to volatilize by dismantling connection.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that rapid tissue freezes pretreatment unit；
Fig. 2 is the sectional structure chart that rapid tissue freezes pretreatment unit；
Fig. 3 is the structural schematic diagram of frozen section prop head；
In figure, 1 cup body, 2 cup lids, 3 cup lid handles, 4 sleeves, 5 frozen section prop heads, the 51 discoid knots of frozen section prop head
Structure, 52 frozen section prop head cylindrical structures, 6 quarter butts, 7 annulus.
Specific embodiment
Clear, complete description is carried out to the technical solution of the utility model with reference to the accompanying drawing, it is clear that described reality
A part of the embodiment that example is the utility model is applied, instead of all the embodiments.Based on the embodiments of the present invention, originally
The every other embodiment that field those of ordinary skill is obtained without making creative work belongs to practical
Novel protection scope.
A kind of rapid tissue provided in this embodiment freezes pretreatment unit, comprising: cylindrical cup body 1, cylindrical cup body 1
For holding isopentane, the device is in use, the isopentane liquid level in cylindrical cup body 1 is higher than frozen section prop head 5
Highly, the embedded flesh tissue being placed in frozen section prop head 5 can sufficiently be submerged.
In the present embodiment, cup lid 2 is set, cup lid 2 is detachably connected with the cylindrical sealing of cup body 1, institute on cylindrical cup body 1
Stating sealed connection is that seal with elastometic washer connects, and the sealed connection of cup lid 2 and cylindrical cup body 1 can prevent quickly waving for isopentane
Hair, avoids harm to the human body.It is noted that the sealed connection mode that can be realized cylindrical cup body 1 and cup lid 2 is equal
It is within the protection scope of the utility model.
In the present embodiment, cup lid is arranged 3 in 2 centre of cup lid, for lifting cup lid 2, facilitates beating for cylindrical cup body 1
It opens and closes.
In the present embodiment, the outside mirror image of cylindrical 1 opening portion of cup body is symmetrical arranged two quarter butts 6, one end of quarter butt 6 with
The outside of cylindrical 1 opening portion of cup body is welded, and it is to have nylon rope on annulus 7 that the other end of quarter butt 6, which is welded with annulus 7, and being used for will
Cylindrical cup body 1 is suspended to liquid nitrogen container inner close fitting liquid nitrogen ullage, and the isopentane in cylindrical cup body 1 is made not contact liquid nitrogen
In the case where by Liquid nitrogen precooler to desired temperature, avoid isopentane crystallization and be not available for solid-state.
In the present embodiment, sleeve 4 is welded at cylindrical 1 bottom centre position of cup body, sleeve 4 is hollow circuit cylinder, sleeve 4
For inserting frozen section prop head 5, frozen section prop head 5 is by frozen section prop head disc-shaped structure 51 and frozen section prop head circle
Rod structure 52 forms, and frozen section prop head 5 is for placing embedded flesh tissue；Frozen section prop head cylindrical structure 52 and set
The centre bore matching of cylinder 4.
Above structure setting inserts frozen section prop head smoothly, and sleeve 4 is that frozen section prop head 5 provides carrier,
Frozen section prop head 5 will not be made crooked, the reliable placement of embedded flesh tissue is ensure that, enable embedded flesh tissue
It is hard to bear cold uniform, it is not in ice crystal and slight crack, the form of the flesh tissue after being cooled is intact.
In the present embodiment, 3, sleeve 4, quarter butt 6 and annulus 7 are all made of stainless steel by cylindrical cup body 1, cup lid 2, cup lid
Material is made, and effectively improves the service life of rapid tissue freezing pretreatment unit.
In the present embodiment, it is highly 70-90mm that the diameter of cylindrical cup body 1, which is 40-60mm,；The diameter of sleeve 4 is 9-
11mm, a height of 20-30mm；The diameter ratio of cylindrical cup body 1 and sleeve 4 is 6.6-3.5,4 height of cylindrical cup body 1 and sleeve
Than for 4.5-2.3；52 diameter of frozen section prop head cylindrical structure is 5-8mm, is highly 20-25mm, frozen section prop head disk
51 diameter of shape structure is 25-30mm, is highly 4-6mm.
In optional embodiment, it is highly 80mm that the diameter of cylindrical cup body 1, which is 50mm,；The diameter of sleeve 4 is 10mm,
Height is 20mm；52 diameter of frozen section prop head cylindrical structure is 8mm, is highly 20mm, frozen section prop head disc-shaped structure
51 diameters are 25mm, are highly 5mm.
In optional embodiment, it is highly 70mm that the diameter of cylindrical cup body 1, which is 40mm,；The diameter of sleeve 4 is 9mm, high
Degree is 25mm；52 diameter of frozen section prop head cylindrical structure is 8mm, is highly 20mm, frozen section prop head disc-shaped structure 51
Diameter is 25mm, is highly 5mm.
In optional embodiment, it is highly 90mm that the diameter of cylindrical cup body 1, which is 60mm,；The diameter of sleeve 4 is 11mm,
Height is 30mm；52 diameter of frozen section prop head cylindrical structure is 8mm, is highly 20mm, frozen section prop head disc-shaped structure
51 diameters are 25mm, are highly 5mm.
In optional embodiment, it is highly 85mm that the diameter of cylindrical cup body 1, which is 55mm,；The diameter of sleeve 4 is
10.5mm is highly 27mm；52 diameter of frozen section prop head cylindrical structure is 8mm, is highly 20mm, frozen section prop head disk
51 diameter of shape structure is 25mm, is highly 5mm.
In above-described embodiment, the components such as cylindrical cup body 1, frozen section prop head disc-shaped structure 52 and described sleeve 4
Various structures are sized to meet the uniform pre-cooling of various sizes of embedded flesh tissue, complete slice with auxiliary.
One of the utility model rapid tissue freeze pretreatment unit use process is as follows:
1) isopentane is poured into cylindrical cup body 1, liquid level 40-60mm covers cup lid 2；
2) nylon rope is tied up on annulus 7, the cylindrical cup body 1 that isopentane is contained in step 1) is suspended in liquid nitrogen container
It is close to liquid nitrogen ullage and 10min is pre-chilled；
3) embedded flesh tissue is placed in frozen section prop head 5, takes out the Sheng of step 2) pre-cooled completion
Cylindrical cup body 1 equipped with isopentane is opened cup lid 2, frozen section prop head 5 is placed in sleeve 4, cylinder cup body 1
Interior isopentane sufficiently submerges the embedded flesh tissue in frozen section prop head 5；
4) cup lid 2 is covered, nylon rope is tied up on annulus 7, cylindrical cup body 1 is suspended to liquid nitrogen container inner close fitting liquid nitrogen liquid
1min is freezed above face；
5) the cylindrical cup body 1 that step 4) freezing is completed is taken out, cup lid 2 is opened, takes out frozen section prop head 5, cut
Piece refunds isopentane in cylindrical cup body 1 in former storage bottle.
The above descriptions are merely preferred embodiments of the present invention, is not intended to limit the utility model, for this
For the technical staff in field, various modifications and changes may be made to the present invention.It is all in the spirit and principles of the utility model
Within, any modification, equivalent replacement, improvement and so on should be included within the scope of protection of this utility model.
Claims (5)
1. a kind of rapid tissue freezes pretreatment unit characterized by comprising
Cylindrical cup body (1), the cylinder cup body (1) is for holding isopentane；
Cup lid (2) are set on the cylinder cup body (1), the cup lid (2) seals with the cylindrical cup body (1) detachably to be connected
It connects, for the isopentane to be closed in the cylindrical cup body (1)；
Cup lid (2) the centre setting cup lid is (3), for lifting the cup lid (2)；
It is welded with sleeve (4) at described cylinder cup body (1) bottom centre position, the sleeve (4) is hollow circuit cylinder, the set
Cylinder (4) is for inserting frozen section prop head (5), and the frozen section prop head (5) is by frozen section prop head disc-shaped structure (51)
It is formed with frozen section prop head cylindrical structure (52), the frozen section prop head (5) is for placing embedded flesh tissue；
It is described cylinder cup body (1) opening portion outside mirror image be symmetrical arranged two quarter butts (6), one end of the quarter butt (6) with
The outside welding of described cylinder cup body (1) opening portion, the other end of the quarter butt (6) are welded with annulus (7), the annulus
It (7) is to have nylon rope on, for the cylindrical cup body (1) to be suspended to liquid nitrogen container inner close fitting liquid nitrogen ullage.
2. rapid tissue according to claim 1 freezes pretreatment unit, it is characterised in that: the cylinder cup body (1),
(3), the sleeve (4), the quarter butt (6) and the annulus (7) are all made of stainless steel by the cup lid (2), the cup lid
Material is made.
3. rapid tissue according to claim 1 freezes pretreatment unit, it is characterised in that: the cylinder cup body (1)
Diameter be 40-60mm, be highly 70-90mm；The diameter of the sleeve (4) is 9-11mm, a height of 20-30mm；The cylinder
Shape cup body (1) and the diameter ratio of the sleeve (4) are 6.6-3.5, the height of cylinder cup body (1) and the sleeve (4)
Than for 4.5-2.3.
4. rapid tissue according to claim 1 freezes pretreatment unit, it is characterised in that: the frozen section prop head circle
Rod structure (52) diameter is 5-8mm, is highly 20-25mm, and frozen section prop head disc-shaped structure (51) diameter is 25-
30mm is highly 4-6mm, and the frozen section prop head cylindrical structure (52) matches with the centre bore of the sleeve (4).
5. rapid tissue according to claim 1 freezes pretreatment unit, it is characterised in that: the cup lid (2) with it is described
The sealed connection of cylindrical cup body (1) is seal with elastometic washer connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821302778.8U CN208520676U (en) | 2018-08-14 | 2018-08-14 | A kind of rapid tissue freezing pretreatment unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821302778.8U CN208520676U (en) | 2018-08-14 | 2018-08-14 | A kind of rapid tissue freezing pretreatment unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208520676U true CN208520676U (en) | 2019-02-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821302778.8U Expired - Fee Related CN208520676U (en) | 2018-08-14 | 2018-08-14 | A kind of rapid tissue freezing pretreatment unit |
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| Country | Link |
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| CN (1) | CN208520676U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111912651A (en) * | 2020-08-24 | 2020-11-10 | 厦门大学附属第一医院 | Preparation method of frozen adipose tissue sections |
-
2018
- 2018-08-14 CN CN201821302778.8U patent/CN208520676U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111912651A (en) * | 2020-08-24 | 2020-11-10 | 厦门大学附属第一医院 | Preparation method of frozen adipose tissue sections |
| CN111912651B (en) * | 2020-08-24 | 2022-04-05 | 厦门大学附属第一医院 | Preparation method of frozen adipose tissue sections |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190219 Termination date: 20190814 |
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| CF01 | Termination of patent right due to non-payment of annual fee |