CN208509801U - A kind of device of solid medium plantation arabidopsis - Google Patents
A kind of device of solid medium plantation arabidopsis Download PDFInfo
- Publication number
- CN208509801U CN208509801U CN201820896995.8U CN201820896995U CN208509801U CN 208509801 U CN208509801 U CN 208509801U CN 201820896995 U CN201820896995 U CN 201820896995U CN 208509801 U CN208509801 U CN 208509801U
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- CN
- China
- Prior art keywords
- solid medium
- arabidopsis
- bottle
- support tube
- plantation
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Mushroom Cultivation (AREA)
Abstract
The utility model relates to a kind of devices of solid medium plantation arabidopsis, including solid medium bottle, support tube and sealed membrane, it is characterized in that the solid medium bottle is transparent Bottle structure, solid medium bottom of bottle portion is provided with solid medium, and bottleneck is connect with support bottom of the tube；The support tube is transparent hollow structure, plays supporting function to Arabidopsis plant, so that it will not lodge；The sealed membrane is set to support tube top, for preventing fungus from entering pollution culture medium.The utility model can be recycled, economic and environment-friendly, and required utensil is easy, operation is convenient, intuitive, and integrated level is high, practical.
Description
Technical field
The utility model relates to a kind of devices of solid medium plantation arabidopsis.
Background technique
Arabidopsis because its growth cycle it is short, genome it is small and be sequenced and as the mode species in plant,
It is the ideal carrier for studying gene function by numerous scholars as preferential research object.And as carrier species, it is necessary to will
Target gene to be studied is transferred in arabidopsis gene group, to obtain transgenic arabidopsis, this also just becomes a kind of fast
Speed understands the means of gene function.The transfection of Agrobacterium flower-dipping method is current a kind of most commonly used method of arabidopsis thaliana transformation, will
The seed obtained after conversion is sowed on the culture medium containing antibiotic-screening, and greening seedling is considered Arabidopsis Mutants F1
In generation, also needs for mutant F1 generation to be selfed after this, to obtain F2 for seed, later to F2 for planting after Seed sterilization
It is implanted on the screening and culturing medium containing antibiotic and is screened, the generation seed of knot is F3 generation, equally by F3 for Seed sterilization
The progress homozygote verifying on the screening and culturing medium containing antibiotic is sowed afterwards, is confirmed whether it is homozygote plant.
Currently, when everybody (is considered F1 generation) after containing greening seedling has been obtained on Antibiotic medium, it is common that will be green
Change seedling, which is transferred in soil, carries out breeding culture, and also having part is to carry out water planting using nutrient solution, but be to ensure that F1 generation is selfed
To F2 generation, it must just to maintain a certain distance that (because arabidopsis is pollinated by pollen, distance closely may be used very much between arabidopsis
Can be carried out the hybridization between different mutants), this is just relatively high to planting site requirement, if carried out between mutant miscellaneous
It hands over, it is then not possible to obtain mutant or can not confirm mutant, inaccuracy is undoubtedly to experiment in this way；Experiment needs will be every
Sowing is screened or is verified on the screening and culturing medium containing antibiotic after the Seed sterilization of a generation, if Seed sterilization is not thorough
Bottom is easy to cause mould contamination, so that it is dead after germination, screening transgenic arabidopsis is influenced very big；In addition, planting
In the process, it needs to add within 3-5 days a water or replacement one time of nutrition liquid, this seems comparatively laborious in the course of cultivation, while soil
Mushroom easy to breed biology or algae, influence the normal growth of arabidopsis in earth or nutrient solution.
Summary of the invention
In view of this, the purpose of this utility model is to provide a kind of device of solid medium plantation arabidopsis, to
Quickly propagating transgenic arabidopsis is from explanation or homozygous generation and obtains aseptic seed.
The utility model is implemented with the following technical solutions to achieve the above object: a kind of solid medium plantation arabidopsis
Device, including solid medium bottle, support tube and sealed membrane, it is characterised in that the solid medium bottle is transparent bottle body
Structure, solid medium bottom of bottle portion are provided with solid medium, and bottleneck is connect with support bottom of the tube；The support tube is
Bright hollow structure plays supporting function to Arabidopsis plant, and so that it will not lodge；The sealed membrane is set to support tube top
End, for preventing fungus from entering pollution culture medium.
Further, the sealed membrane is plastics film structure, and intermediate position is additionally provided with bacterial isolation layer.
Further, the bacterial isolation layer is cross hatch composition, and gap is 0.22 μm, while barrier mushroom enters,
Air and moisture can be allowed freely to enter and leave again.
Further, the support tube is cylindrical structure, is additionally provided with a bulge-structure close to tip side.
Further, the solid medium bottle is cylindrical structure.
Further, the solid medium bottle and support tube are sealed by transparent adhesive tape connects.
The utility model has the advantages that compared with prior art
The utility model can be recycled, economic and environment-friendly, and required utensil is easy, operation is convenient, intuitive, and integrated level is high,
It is practical.
Detailed description of the invention
Fig. 1 is the utility model structure diagram
Fig. 2 is the utility model sealing membrance structure schematic diagram
Fig. 3 is the utility model supporting tube structure schematic diagram
Fig. 4 is the utility model solid medium bottle structure schematic diagram
In figure: 1- bacterial isolation layer, 2- sealed membrane, 3- support tube bulge-structure, 4- support tube, 5- Arabidopsis plant, 6- solid
Medium bottle, 7- solid medium.
Specific embodiment
The utility model is described further with reference to the accompanying drawings and embodiments.
Please referring to Fig. 1, the present embodiment provides a kind of devices of solid medium plantation arabidopsis, including solid medium bottle
6, support tube 4 and sealed membrane 2, it is characterised in that the solid medium bottle 6 is transparent Bottle structure, the solid medium
6 bottoms of bottle are provided with solid medium 7, and bottleneck is connect with support bottom of the tube 4；The support tube 4 is transparent hollow structure, right
Arabidopsis plant plays supporting function, and so that it will not lodge；The sealed membrane 2 is set to 4 top of support tube, for preventing fungus
Into pollution culture medium.
In one embodiment of the invention, further, the sealed membrane 2 be plastics film structure, long 100mm, wide 100mm,
High 0.1mm, intermediate position are additionally provided with bacterial isolation layer.
In one embodiment of the invention, further, the bacterial isolation layer is cross hatch composition, and device gap is 0.22 μm, is being hindered
While mushroom enters, and air and moisture can be allowed freely to enter and leave.
In one embodiment of the invention, further, the support tube 4 is cylindrical structure, and outer diameter 31mm is high
300mm, wall thickness 1mm；Being additionally provided with a bulge-structure radius close to tip side is 32mm, high 1mm, thickness 1mm.Stay pipe 4 was both
Light can be allowed to pass through, be supplied to plant photosynthesis, and supporting function can be played to Arabidopsis plant, so that it will not lodge.
Further in one embodiment of the invention, the solid medium bottle 6 is cylindrical structure, and bottom surface radius is
31 millimeters (mm), ground thickness 2mm, high 100mm, wall thickness 1mm.Solid medium (7) formula is 4.43 g/L MS and 8 g/L fine jades
Cosmetics sterilizes 15 minutes at 121 DEG C, and volume is 141 ml.
Further in one embodiment of the invention, the solid medium bottle 6, which is sealed with support tube 4 by transparent adhesive tape, to be connected
It connects.
In order to allow those skilled in the art to better understand the technical solution of the utility model, below in conjunction with the utility model
Embodiment schematic diagram describes to the utility model practical application in detail.
It needs to sterilize all accessories in operation referring to attached drawing 1, and completes following work in superclean bench: first
Greening seedling single plant under antibiotic-screening culture medium is transferred on the solid medium of solid medium plantation arabidopsis device,
Then merge stay pipe with solid medium bottle, and seal gap between the two with transparent adhesive tape, is covered later with sealed membrane
Stay pipe, and tightening sealed membrane with lower end of the rope at stay pipe upper lobes prevents fungus from entering pollution culture medium.Later
The device is put into 23 DEG C, relative humidity 60-70%, under the environmental condition of illumination 16h, during the growth process without to it
It the management such as watered, applied fertilizer, sterile arabidopsis seed can be collected into after 20-30 days.
The above is only the preferred embodiment of the present invention, it is all done according to present utility model application the scope of the patents it is equal
Deng variation and modification, it should all belong to the covering scope of the utility model.
Claims (5)
1. a kind of device of solid medium plantation arabidopsis, including solid medium bottle, support tube and sealed membrane, feature exist
It is transparent Bottle structure in the solid medium bottle, solid medium bottom of bottle portion is provided with solid medium, bottleneck
It is connect with support bottom of the tube；The support tube is transparent hollow structure, plays supporting function to Arabidopsis plant, so that it will not
Lodging；The sealed membrane is set to support tube top, for preventing fungus from entering pollution culture medium；The sealed membrane is plastic layer
Membrane structure, intermediate position are additionally provided with bacterial isolation layer.
2. a kind of device of solid medium plantation arabidopsis according to claim 1, it is characterised in that: the bacterial isolation layer
For cross hatch composition, gap is 0.22 μm, while barrier mushroom enters, and air and moisture can be allowed freely to enter and leave.
3. a kind of device of solid medium plantation arabidopsis according to claim 1, it is characterised in that: the support tube
For cylindrical structure, a bulge-structure is additionally provided with close to tip side.
4. a kind of device of solid medium plantation arabidopsis according to claim 1, it is characterised in that: the solid training
Supporting base bottle is cylindrical structure.
5. a kind of device of solid medium plantation arabidopsis according to claim 1, it is characterised in that: the solid training
It supports base bottle and support tube and connection is sealed by transparent adhesive tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820896995.8U CN208509801U (en) | 2018-06-11 | 2018-06-11 | A kind of device of solid medium plantation arabidopsis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820896995.8U CN208509801U (en) | 2018-06-11 | 2018-06-11 | A kind of device of solid medium plantation arabidopsis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208509801U true CN208509801U (en) | 2019-02-19 |
Family
ID=65324818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820896995.8U Expired - Fee Related CN208509801U (en) | 2018-06-11 | 2018-06-11 | A kind of device of solid medium plantation arabidopsis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208509801U (en) |
-
2018
- 2018-06-11 CN CN201820896995.8U patent/CN208509801U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190219 Termination date: 20210611 |
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| CF01 | Termination of patent right due to non-payment of annual fee |