CN208399514U - A kind of micro-fluidic immunoassay device - Google Patents
A kind of micro-fluidic immunoassay device Download PDFInfo
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- CN208399514U CN208399514U CN201821193255.4U CN201821193255U CN208399514U CN 208399514 U CN208399514 U CN 208399514U CN 201821193255 U CN201821193255 U CN 201821193255U CN 208399514 U CN208399514 U CN 208399514U
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Abstract
The utility model discloses a kind of micro-fluidic immunoassay devices, including chip upper cover setting up and down and lower layer chip, detection module is distributed along the circumference on the upside of lower layer chip, first pair of lighting module and second pair of lighting module, detection module, first pair of lighting module and second pair of lighting module include the reagent filling pond being sequentially communicated and the first buffer channel and the injection port being sequentially communicated, sample storage pond, second buffer channel, reaction detection pond, third buffer channel, waste liquid pool and the 4th buffer channel, first buffer channel is connected to reaction detection pond, the rear side in sample storage pond is equipped with serum filter membrane, the rear side in reaction detection pond is equipped with filter membrane, 4th buffer channel of detection module, 4th buffer channel of first pair of lighting module and the 4th buffer channel of second pair of lighting module are connected to pump interface.The processing time of sample can not only be greatly shortened for the utility model, and cost is greatly lowered, moreover it is possible to improve detection accuracy, realize mobile detection and the self-service detection function of family.
Description
Technical field
The utility model relates to immunoassay device technical fields, more particularly to a kind of micro-fluidic immunoassay device.
Background technique
Stomach trouble is a clock disease very common during we live, and as the saying goes " ten stomaches, nine disease ", stomach trouble is still difficult at this stage
With one of the disease of radical cure, mainly empirically and scope, however scope detection needs directly general to the diagnosis status of stomach trouble at present
Electronic equipment protrudes into the human body, brings many pains to patient.Therefore, studying external diagnostic means is highly desirable,
In the means of in-vitro diagnosis, serological method and index are very convenient, cheap, painless detection means, in recent years serum
Learning Indexs measure has the tendency that increasing year by year, and Gastrin-17 (Gastrin-17) has filled up stomachial secretion in the inspection of serology stomach trouble
The blank of functional parameter.
Gastrin is a kind of gastrointestinal hormone mainly secreted by antrum and duodenal G cell, to adjusting alimentary canal function
Can and its structural integrity be maintained to play an important role.Presently found gastrin molecule, including gastrin is former, glycine extended stomach
The multiple types such as secretin and amidated gastrin.Wherein, the gastrin for having bioactivity of 95% or more human body is α-amidation
Gastrin, mainly containing two kinds of isomers Gastrin-17 (Gastrin-17, abbreviation G-17) and Gastrin-34 (gastrin -34,
Abbreviation G-34), wherein 80%-90% is G-17.The secretion of G-17 is mainly influenced by stomach inner pH value, G cell quantity and feed,
The index can determine whether stomachial secretion function, and position, degree and the risk of reflection gastric acid height, prompt Mucosal atrophy can be more complete
Face judges stomach lining health status.
The advantages of G-17 diagnosis of gastrosis: it is simple and easy, reproducible, have no adverse reaction, be economical, interpret it is convenient.Regular body
G-17 inspection is done in inspection, screening disease of stomach, understanding stomach health status early can prevent early treatment early.
Microfluidic chip technology, also known as chip lab (Lab on a Chip) are a kind of biology, chemistry, medicine
The basic operation units such as sample preparation, reaction, separation, the detection of analytic process are integrated on one piece several square centimeters of chip,
It is automatically performed the technology of analysis overall process.Chip is mainly made of microchannel, Micropump, micro-valve, reaction microchamber room etc., has height
It is integrated, automation and Biochemical Lab various functional characteristics.The detection of G-17 at present mainly have radio immunoassay,
Not yet there is the micro-fluidic chip immunoassay device of G-17 in fluorescence immune chromatography method, zymetology immunization and chemoluminescence method etc..
Utility model content
The purpose of the utility model is to provide a kind of micro-fluidic immunoassay devices, above-mentioned of the existing technology to solve
Problem is greatly shortened the processing time of sample, improves the precision of detection.
To achieve the above object, the utility model provides following scheme:
The utility model provides a kind of micro-fluidic immunoassay device, including chip upper cover and lower layer's core setting up and down
Piece is distributed along the circumference with detection module, first pair of lighting module and second pair of lighting module, the detection on the upside of the lower layer chip
Module, first pair of lighting module and second pair of lighting module include that the reagent filling pond being sequentially communicated and the first buffering are logical
Road and the injection port being sequentially communicated, sample storage pond, the second buffer channel, reaction detection pond, third buffer channel, waste liquid pool and
Four buffer channels, first buffer channel are connected to the reaction detection pond, and the rear side in the sample storage pond is filtered equipped with serum
Film, the rear side in the reaction detection pond are equipped with filter membrane, the 4th buffer channel of the detection module, first control
The 4th buffer channel of module and the 4th buffer channel of second pair of lighting module are connected to pump interface.
Preferably, reagent A, the institute of first pair of lighting module are embedded in the reaction detection pond of the detection module
It states and is embedded with the first antigen in reaction detection pond, it is anti-that second is embedded in the reaction detection pond of second pair of lighting module
It is pre-buried in original, the reagent filling pond of first pair of lighting module and the reagent filling pond of second pair of lighting module
There is reagent B.
Preferably, the detection module, first pair of lighting module and second pair of lighting module are one or more groups of.
Preferably, the chip upper cover and lower layer chip are disk.
Preferably, the pump interface is set to the center point of the disk.
Preferably, the length of first buffer channel is greater than the length of second buffer channel.
Preferably, the material of the chip upper cover and the lower layer chip is in glass, PDMS, PC plastic and PMMA
Any one.
Preferably, it offers rubber stopper from top to bottom on lid on the chip to get stuck and rubber consent, the rubber consent
Get stuck with the rubber stopper and be sealedly connected with a rubber stopper, the rubber stopper is used to be inserted into the hole needle of transfer tube, the hole needle with
The pump interface connection.
Preferably, the rubber consent and the rubber stopper, which get stuck, is respectively positioned at the center of the chip upper cover.
Preferably, the rubber consent and the pump interface are coaxially disposed.
The utility model achieves following technical effect compared with the existing technology:
1, the immune detection of Gastrin-17 sample carries out in the lower layer chip of micro-fluidic immunoassay device, has sample
Present treatment easily and fast, it is at low cost the advantages that.
2, under micro-scale, the specific surface area of sample is much larger than macroscopical sample, can be more within very short time
The lower biochemical reaction that time-consuming of many macroscopic views is adequately completed, and possesses the detection knot more more accurate than traditional detection means
Fruit.
3, the processing time of sample can not only be greatly shortened for micro-fluidic immunoassay device, biological principle, that is, antibody and
Instrument cost is greatly lowered, moreover it is possible to improve the precision of detection, realize the functions such as the self-service detection of mobile detection and family.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only the utility model
Some embodiments for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other attached drawings.
Fig. 1 is the structural schematic diagram of the micro-fluidic immunoassay device of the utility model；
Fig. 2 is the top view of the utility model chip upper cover；
Fig. 3 is the main view of the utility model chip upper cover；
Fig. 4 is the structural schematic diagram of the detection module of the utility model lower layer chip；
The structural representation that Fig. 5 is detection module in lower layer chip, first pair of lighting module and second pair of lighting module when being one group
Figure；
The structural representation that Fig. 6 is detection module in lower layer chip, first pair of lighting module and second pair of lighting module when being two groups
Figure；
The structural representation that Fig. 7 is detection module in lower layer chip, first pair of lighting module and second pair of lighting module when being three groups
Figure；
Wherein: 1- chip upper cover, 2- lower layer chip, 3- detection module, 4- first to lighting module, 5- second to lighting module,
6- rubber stopper gets stuck, 7- rubber consent, and 8- reagent fills pond, the first buffer channel of 9-, 10- injection port, 11- sample storage pond, 12- blood
Clear filter membrane, the second buffer channel of 13-, 14- reaction detection pond, 15- filter membrane, 16- third buffer channel, 17- waste liquid pool,
The 4th buffer channel of 18-, 19- pump interface.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are in the premise not made the creative labor
Under every other embodiment obtained, fall within the protection scope of the utility model.
In the description of the utility model it is to be appreciated that term " on ", "lower", " left side " and " right side " instruction orientation or
Positional relationship is orientation and positional relationship based on the figure, it is only for facilitate the structurally and operationally mode of description, and
It is not to indicate or imply that signified part must have a particular orientation, with the operation of specific orientation, thus should not be understood as
Limitations of the present invention.
The purpose of the utility model is to provide a kind of micro-fluidic immunoassay devices, to solve of the existing technology ask
Topic, is greatly shortened the processing time of sample, improves the precision of detection.
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, with reference to the accompanying drawing and have
Body embodiment is described in further detail the utility model.
As shown in Fig. 1-Fig. 7: a kind of micro-fluidic immunoassay device applied to Gastrin-17 is present embodiments provided,
With included calibration system, it is ensured that testing result is accurate, including chip upper cover 1 and lower layer chip 2 setting up and down, chip
Upper cover 1 and lower layer chip 2 are disk.Micro-fluidic immunoassay device can pass through injection molding, photoetching, burn into revolving die, coining etc.
Method is fabricated, and is carried out chemical modification to the surface texture of lower layer chip according to demand after completing, be can be hydrophilic change
Microchannel surface can be modified as water-wetted surface by process for modifying surface by property or hydrophobically modified, and fluid is in the channel
Resistance can become smaller, and also avoiding fluid residuals cannot flow completely out in runner.The material of chip upper cover 1 and lower layer chip 2 is equal
For any one or a few in glass, PDMS, PC plastic and PMMA.Chip upper cover 1 and lower layer chip 2 are preferably by bonding
Encapsulation, specifically, the bonding of chip upper cover 1 and lower layer chip 2 prepares the difference of material according to chip and selects different bondings
Mode.Bonding pattern is preferably Direct Bonding encapsulation, ultrasonic bond encapsulation, laser bonding encapsulation, solution auxiliary bonding packaging, heat
Bonding packaging introduces Intermediate Layer Bonding encapsulation by glue or film etc..
Rubber stopper is offered in chip upper cover 1 from top to bottom to get stuck 6 and rubber consent 7, rubber consent 7 gets stuck with rubber stopper
6 are sealedly connected with a rubber stopper, and rubber stopper is used to be inserted into the hole needle of transfer tube, and hole needle is connected to pump interface 19.7 He of rubber consent
Rubber stopper, which gets stuck, 6 to be respectively positioned at the center of chip upper cover 1.Rubber stopper is preferably Medical rubber plug, and guarantee is not in that gas is let out
The phenomenon that leakage and liquid are flow backwards.The external vacuum pump of lower layer chip 2 vacuumizes, so that the fluid both ends in 2 channel of lower layer chip have
There is pressure difference, is conducive to the flowing of fluid.
The upside of lower layer chip 2 is distributed along the circumference with 3, first pairs of lighting modules 4 of detection module and second pair of lighting module 5.Inspection
It surveys 3, first pairs of lighting modules 4 of module and second pair of lighting module 5 is identical in structure, detection module 3 is used to carry out sample
Detection, first pair of lighting module 4 and second pair of lighting module 5 compare position respectively as the height of calibration and space bit compares.Detect mould
3, first pairs of lighting modules 4 of block and second pair of lighting module 5 include the reagent filling pond 8 being sequentially communicated and the first buffer channel 9 with
And injection port 10, sample storage pond 11, the second buffer channel 13, reaction detection pond 14, third buffer channel 16, waste liquid being sequentially communicated
Pond 17 and the 4th buffer channel 18, the first buffer channel 9 are connected to reaction detection pond 14, and the rear side in sample storage pond 11 is equipped with serum mistake
The rear side of filter membrane 12, reaction detection pond 14 is equipped with filter membrane 15,18, first pairs of lighting modules 4 of the 4th buffer channel of detection module 3
The 4th buffer channel 18 and the 4th buffer channel 18 of second pair of lighting module 5 be connected to pump interface 19, pump interface 19 is not only
The access port that can be used as vacuum pump is also used as reacting in 3, first pairs of lighting modules 4 of detection module and second pair of lighting module 5
The public waste liquid pool of Excess reagents afterwards.The length of first buffer channel 9 is greater than the length of the second buffer channel 13.Pump interface 19
It is set to the center point of lower layer chip 2, rubber consent 7 and pump interface 19 are coaxially disposed.
Reagent A is embedded in the reaction detection pond 14 of detection module 3 according to actual needs, reagent A preferably captures antibody.
The first antigen, the reaction inspection of second pair of lighting module 5 are embedded in the reaction detection pond 14 of first pair of lighting module 4 according to actual needs
It surveys in pond 14 and is embedded with the second antigen according to actual needs, the concentration of the first antigen and the second antigen is different.First pair of lighting module 4
Reagent filling pond 8 and second pair of lighting module 5 reagent filling pond 8 in be embedded with reagent B, reagent B is preferably coupled fluorescence
The labelled antibody of element.The pre-buried filling of reagent A, reagent B, the first antigen and the second antigen can lead to specific reference to the difference of reagent
Cross physical absorption, chemical crosslinking, sol-gel embedding, in the modes such as microballon is fixed, film is fixed and is freezed any one or it is several
The reagent that kind is embedded into 14, first pairs of the reaction detection pond lighting module 4 of detection module 3 respectively fills pond 8 and second pair of lighting module 5
Reagent filling 8, first pairs of pond lighting module 4 reaction detection pond 14 and second pair of lighting module 5 reaction detection pond 14 in.
It is quantitative into whole blood sample, first pair of lighting module 4 and the second control mould in the injection port 10 of detection module 3 when detection
The injection port 10 of block 5 not sample introduction sheet.Specifically, using quantitative capillary tube or other equipment to the detection mould of lower layer chip 2
Quantitative whole blood or serum sample are injected in block 3.
In addition, 3, first pairs of lighting modules 4 of detection module and second pair of lighting module 5 may be arranged as one group, can also set
It is set to multiple groups, preferably two groups or three groups.3, first pairs of lighting modules 4 of multiple groups detection module and second pair of lighting module 5 circuit sequentially
Connection, is co-located on the upside of lower layer chip 2.3, first pairs of lighting modules 4 of multiple groups detection module and second pair of lighting module 5 are realized
In single lower layer chip 2 multiple detection systems are designed, while making the sample of many cases pattern detection or multinomial different indexs
Detection, it is easy to operate, shorten detection time.
The course of work of micro-fluidic immunoassay device is as follows:
Lower layer chip 2 is vacuumized in the external vacuum pump of pump interface 19 of 2 the center point of lower layer chip.The reaction of detection module
Process: the fluid both ends in 3 channel of detection module have certain pressure difference, and the effect of pressure difference can make fluid in microchannel
Middle flowing, the distance of flowing is related with the coefficient of viscosity etc. of microchannel surface roughness, fluid, and whole blood sample is in surface tension
Effect is lower to flow into sample storage pond 11, flows into reaction by the second buffer channel 13 by the filtered serum sample of serum filter membrane 12
In detection cell 14, and with reagent A association reaction pre-buried in reaction detection pond 14, stop the extraction of vacuum pump in reaction process,
When stopping driving, fluid can stop no longer to be flowed in situ vacuum pump；During vacuum pump vacuumizes, reagent is filled out
The reagent B filled in pond 8 can also be flowed to reaction detection pond 14, but since the length of the first buffer channel 9 is logical more than the second buffering
Road 13 is long, so that reagent B is also trapped in the first buffer channel 9 when serum sample enters reaction detection pond 14 and reacted,
After waiting serum sample sufficiently to react with reagent A pre-buried in reaction detection pond 14, it is again started up vacuum pump and vacuumizes, it will be extra
Reaction after sample take and flow into waste liquid pool away, while pre-filled reagent B is transported in reaction detection pond 14 and sample
It is reacted, is again turned on vacuum pump, the reagent for reacting extra is siphoned away, so that Excess reagents are flowed by third buffer channel 16
Enter in waste liquid pool 17.The reaction process of first pair of lighting module 4 and second pair of lighting module 5: due to first pair of lighting module 4 and second pair
The injection port 10 of lighting module 5 not sample introduction sheet, the whole blood sample in the injection port 10 because detection module 3 may be not present flow into reaction
The process of detection cell 14, remaining process referring to detection module 3 reaction process.3, first couples of 4 and of lighting module of detection module
It is common waste that the reagent of second pair of lighting module 5 after the reaction was completed, which flows into pump interface 19 by respective 4th buffer channel 18 respectively,
In liquid pool.Using fluorescence detecting system respectively to the reaction detection of 14, the first pairs of lighting modules 4 in the reaction detection pond of detection module 3
Pond 14 and the reaction detection pond 14 of second pair of lighting module 5 are detected, and the data transmission after will test is carried out into pertinent instruments
Processing and display.
The reaction detection principle of micro-fluidic immunoassay device is double-antibody method principle: using being connected on solid phase carrier
Antibody and enzyme labelled antibody respectively in sample be detected antigen molecule on two antigenic determinants combine, formation solid phase antibody-
Antigen-enzyme labelled antibody immune complex.Since the amount of solid phase antibody and enzyme labelled antibody is relative to determined antigen in reaction system
Excessive, therefore, the forming amount of compound and the content of determined antigen are directly proportional (can detect in range in method).It measures compound
The colored substance quality (OD value) that enzyme effect in object generates after the substrate of addition, that is, can determine determined antigen content.If solid phase
Antibody and enzyme labelled antibody on carrier are detected on antigen molecule in conjunction with two different antigenic determinants, then from sample respectively
Belong to two-site sandwich method.According on solid phase carrier antigen and enzyme-labelled antigen respectively in sample be detected antibody molecule knot
It closes, is then dual-antigen sandwich method.
In the present embodiment using micro-fluidic immunoassay device to sample carry out immune detection, greatly reduce sample and
The dosage of reagent, it is only necessary to which the whole blood or serum of 1-100 μ L can complete detection process, and corresponding reagent dosage is also very big
Reduction, reduce the sample process time；The serum filter membrane being added in the chips, can quickly separate serum, overcome
Tradition using centrifugation serum isolation technics time-consuming the characteristics of, improve the efficiency of sample preprocessing；Detecting instrument microminiaturization, just
Taking may be implemented the universal detection mode of clinical and mobile detection or family, bring to medical worker and patient
It greatly facilitates；On the micro scale, the specific surface area of reagent and sample is much larger than under macro-scale, the combination of reagent and sample
Can be more sufficiently and rapid, therefore detection time also greatly reduces, can more fully complete within very short time
The lower biochemical reaction that time-consuming of many macroscopic views, and possess the testing result more more accurate than traditional detection means；It can not only incite somebody to action
The processing time of sample is greatly shortened, and biological principle, that is, antibody and instrument cost are greatly lowered, moreover it is possible to improve the essence of detection
Accuracy realizes the functions such as the self-service detection of mobile detection and family.
It applies specific case in this specification to be expounded the principles of the present invention and embodiment, the above reality
The explanation for applying example is merely used to help understand the method and its core concept of the utility model；Meanwhile for the general of this field
Technical staff, based on the idea of the present invention, there will be changes in the specific implementation manner and application range.To sum up institute
It states, the content of the present specification should not be construed as a limitation of the present invention.
Claims (10)
1. a kind of micro-fluidic immunoassay device, it is characterised in that: described including chip upper cover setting up and down and lower layer chip
Detection module, first pair of lighting module and second pair of lighting module, the detection module, institute are distributed along the circumference on the upside of lower layer chip
State first pair of lighting module and second pair of lighting module include be sequentially communicated reagent filling pond and the first buffer channel and
Injection port, sample storage pond, the second buffer channel, reaction detection pond, third buffer channel, waste liquid pool and the 4th buffering being sequentially communicated
Channel, first buffer channel are connected to the reaction detection pond, and the rear side in the sample storage pond is equipped with serum filter membrane, described
The rear side in reaction detection pond is equipped with filter membrane, the 4th buffer channel of the detection module, first pair of lighting module
The 4th buffer channel of 4th buffer channel and second pair of lighting module is connected to pump interface.
2. micro-fluidic immunoassay device according to claim 1, it is characterised in that: the reaction of the detection module
It is embedded with reagent A in detection cell, is embedded with the first antigen in the reaction detection pond of first pair of lighting module, described second
To the second antigen is embedded in the reaction detection pond of lighting module, the reagent of first pair of lighting module fills Chi Hesuo
It states in the reagent filling pond of second pair of lighting module and is embedded with reagent B.
3. micro-fluidic immunoassay device according to claim 1, it is characterised in that: the detection module, described first
It is one or more groups of to lighting module and second pair of lighting module.
4. micro-fluidic immunoassay device according to claim 1, it is characterised in that: the chip upper cover and lower layer chip
It is disk.
5. micro-fluidic immunoassay device according to claim 4, it is characterised in that: the pump interface is set to the circle
The center point of disk.
6. micro-fluidic immunoassay device according to claim 1, it is characterised in that: the length of first buffer channel
Greater than the length of second buffer channel.
7. micro-fluidic immunoassay device according to claim 1, it is characterised in that: the chip upper cover and the lower layer
The material of chip is any one in glass, PDMS, PC plastic and PMMA.
8. micro-fluidic immunoassay device according to claim 1, it is characterised in that: on the chip on lid from top to bottom
It offers rubber stopper to get stuck and rubber consent, the rubber consent and the rubber stopper get stuck and be sealedly connected with a rubber stopper, institute
It states rubber stopper and is connected to for being inserted into the hole needle of transfer tube, the hole needle with the pump interface.
9. micro-fluidic immunoassay device according to claim 8, it is characterised in that: the rubber consent and the rubber
Fork clip shell is respectively positioned at the center of the chip upper cover.
10. micro-fluidic immunoassay device according to claim 8, it is characterised in that: the rubber consent and the pump
Interface coaxial arrangement.
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| CN201821193255.4U CN208399514U (en) | 2018-07-26 | 2018-07-26 | A kind of micro-fluidic immunoassay device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308296A (en) * | 2019-07-10 | 2019-10-08 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory |
| WO2022257007A1 (en) * | 2021-06-08 | 2022-12-15 | 京东方科技集团股份有限公司 | First substrate, microfluidic chip, and sample processing method |
-
2018
- 2018-07-26 CN CN201821193255.4U patent/CN208399514U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308296A (en) * | 2019-07-10 | 2019-10-08 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory |
| WO2022257007A1 (en) * | 2021-06-08 | 2022-12-15 | 京东方科技集团股份有限公司 | First substrate, microfluidic chip, and sample processing method |
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