CN208362313U - High-throughput nucleic acid extracts and purification devices - Google Patents
High-throughput nucleic acid extracts and purification devices Download PDFInfo
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- CN208362313U CN208362313U CN201820710302.1U CN201820710302U CN208362313U CN 208362313 U CN208362313 U CN 208362313U CN 201820710302 U CN201820710302 U CN 201820710302U CN 208362313 U CN208362313 U CN 208362313U
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- component
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- nucleic acid
- bar magnet
- bracket
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Abstract
The utility model discloses a kind of high-throughput nucleic acid and extracts and purification devices, including vibration component, bar magnet, it is connected to an at least bracket for vibration component, and it is fixed on at least shell-like component on bracket, vibration component is configured to drive bracket and the vibration of shell-like component, bar magnet is movably contained in shell-like component, bracket has first position and the second position, at first position, bar magnet can be contained in shell-like component to adsorb magnetic bead, and at the second position, bar magnet can be separated with shell-like component with desorption magnetic bead.
Description
Technical field
The utility model relates to biometrics fields more particularly to a kind of nucleic acid of high throughput automatically to extract and purify
Device.
Background technique
Super-paramagnetism nano microballoon is a kind of novel functional material.Its inside is a magnetic core, thus in external magnetic
Under the action of, microballoon can be with displacement；Outside is one layer of clad, and surface is dispersed with many active groups, can with it is thin
The biochemical reagents such as born of the same parents, protein, nucleic acid, enzyme are coupled, and then realize separation under the influence of a magnetic field.Magnetic microsphere is in biochemistry
The application that analysis field is succeeded.In recent years, magnetic microsphere is coated with upper specific antibody, receptor, single stranded DNA, for dividing
From the target body in complex sample, immense success is obtained.Compared with traditional separation method, magnetic microsphere for complex component
The separation of biological sample can be realized separation and enrichment while carry out, substantially increases separating rate and bioaccumulation efficiency, while
Greatly improve the sensitivity of analysis detection.This magnetic microsphere has been widely used in immunoassay, nucleic acid separation and Extraction, thin
The multiple fields such as born of the same parents' sorting, the fixation of enzyme.Magnetic microsphere is applied to the trace microorganism or certain in detection environmental sample
Active chemistry achieves good effect.
But nanometer magnetic bead Shortcomings in use:
1. nanometer magnetic bead is larger to the dependence of equipment, departing from equipment, the use of magnetic bead will be comparatively laborious, the cost of equipment
And the defect of ease for use limits the universal of the technology；
2. during magnetic bead is mixed with solution, cannot mix well, easily agglomerates, reduce extraction purification efficiency.
Summary of the invention
The problem of utility model aims to solve magnetic bead mixing and magnetic bead ease for use.
To achieve the above object, the utility model provides a kind of nucleic acid extraction and purifier apparatus, including vibration component, magnetic
Stick, is connected to an at least bracket for vibration component, and at least a shell-like component, the vibration component that are fixed on bracket are configured to
Bracket and the vibration of shell-like component, bar magnet is driven to be movably contained in shell-like component, bracket has first position and second
It sets, at first position, bar magnet can be contained in shell-like component to adsorb magnetic bead, and at the second position, bar magnet can be with shell-like component
Separation is with desorption magnetic bead.
One end of shell-like component is silent as a further improvement of the utility model, and the opposite other end is opening.
Vibration component is configured to be controlled by four spindle motors as a further improvement of the utility model,.
Nucleic acid extraction and purifier apparatus further include workbench, workbench configuration as a further improvement of the utility model,
Sample hole location is to accommodate sample.
Sample hole location includes the first sample hole location and the second sample hole location, branch as a further improvement of the utility model,
When frame is located at first position, shell-like component corresponds to the first sample hole location, and when bracket is located at the second position, shell-like component corresponds to
Second sample hole location.
Nucleic acid extraction and purifier apparatus further include air path system as a further improvement of the utility model, to work
Platform air-supply.
Nucleic acid extraction and purifier apparatus as a further improvement of the utility model, which is characterized in that further include heated mould
Block.
Compared with prior art, the utility model by bar magnet be movably contained in bar magnet set in, by bar magnet merging or
It takes out realize that magnetic bead is connect in the transfer of different sample rooms, while by bar magnet set with vibration component with can be convenient in bar magnet set,
The vibration that can be covered by bar magnet mixes the magnetic bead in sample.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the extraction of the utility model high-throughput nucleic acid and one embodiment of purifier apparatus；
Fig. 2 is the use state of insertion bar magnet in the bar magnet set of the extraction of the utility model high-throughput nucleic acid and purifier apparatus
Figure；
Fig. 3 is the use state of extraction bar magnet in the bar magnet set of the extraction of the utility model high-throughput nucleic acid and purifier apparatus
Figure.
Specific embodiment
The utility model is described in detail below with reference to specific embodiment shown in the drawings.But these embodiment party
Formula is not intended to limit the utility model, structure that those skilled in the art are made according to these embodiments, method or
Transformation functionally is all contained in the protection scope of the utility model.
Join Fig. 1, high-throughput nucleic acid extracts and purifier apparatus, including vibration component, workbench, air path system and shell.
Specifically, vibration component is controlled by four spindle motors, it can be achieved that front and rear, left and right, up and down motion, for shift magnetic bead,
Mix magnetic bead.
Workbench has multiple stations, can accommodate multiple sample panels simultaneously, realize multi-step oneself for placing sample panel
Dynamicization is extracted.Wherein, part or all of station setting heating module is, it can be achieved that temperature is adjusted, to sample heating or refrigeration.
Air path system be two groups or more fan form, to workbench blow, with during the extraction process dry magnetic bead with
And exclude aerosol.
Above-mentioned component and programme-controlled or computer are electrically connected, and automate extraction process with controlled completion.
Shell is located at the outside of complete machine, and shell is enclosed an accommodating chamber to accommodate, and door body is arranged to pivot or move in shell
Dynamic opening and closing accommodating chamber.
Vibration component connects bar magnet and covers bracket, fixes several bar magnet sets on bar magnet set bracket, and bar magnet is towards the end of sample well
Portion is movably sheathed in bar magnet set.Join Fig. 2 and Fig. 3, it is to close towards one end of sample well that bar magnet set 10, which is shell-like component,
Mouthful, opposite other end is opening, and material is plastics or other non-magnetic materials, it acts on as carrying bar magnet 20 to mix magnetic bead 30,
And the liquid environment of isolation bar magnet 20 and sample 40.Magnetic bead 30 is scattered in sample 40.Vibration component moves bar magnet set branch
Bar magnet set 10 is placed in different sample wells by frame, and is transmitted to vibration energy by bar magnet set bracket and bar magnet set 10
In sample 40, magnetic bead 30 is mixed.Join Fig. 2, when bar magnet 20 is located in bar magnet set 10, mobile bracket protrudes into bar magnet set 10
In a sample 40, the magnetic bead 30 in the sample 40 can be adsorbed on 10 outside of bar magnet set, when bar magnet set bracket moves into another sample
When, magnetic bead 30 shifts therewith, then bar magnet 20 is detached from bar magnet set 10, and magnetic bead 30 loses magnetic attraction, from 10 outside of bar magnet set
Desorption.The purpose by magnetic bead 30 from a sample well bit transition to another sample hole location so can be achieved.
The applicable sample type of the utility model is a variety of, including tissue, blood, PCR sample, plasmid gene group etc..
It should be appreciated that although this specification is described in terms of embodiments, but not each embodiment only includes one
A independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should will say
As a whole, the technical solution in each embodiment may also be suitably combined to form those skilled in the art can for bright book
With the other embodiments of understanding.
Tool of the series of detailed descriptions listed above only for the feasible embodiment of the utility model
Body explanation, they are all without departing from made by the utility model skill spirit not to limit the protection scope of the utility model
Equivalent implementations or change should be included within the scope of protection of this utility model.
Claims (7)
1. a kind of high-throughput nucleic acid extracts and purification devices, which is characterized in that including
Vibration component,
Bar magnet,
It is connected to an at least bracket for vibration component, and
At least shell-like component being fixed on bracket,
The vibration component is configured to that the bracket and shell-like component is driven to shake,
The bar magnet is movably contained in the shell-like component,
The bracket has first position and the second position, and bar magnet can be contained in the shell-like component at the first position
To adsorb magnetic bead, bar magnet can be separated with the shell-like component with magnetic bead described in desorption in the second position.
2. high-throughput nucleic acid according to claim 1 extracts and purification devices, which is characterized in that the one of the shell-like component
End is that silent, the opposite other end is opening.
3. high-throughput nucleic acid according to claim 1 extracts and purification devices, which is characterized in that the vibration component configuration
To be controlled by four spindle motors.
4. high-throughput nucleic acid according to claim 1 extracts and purification devices, which is characterized in that it further include workbench, institute
Workbench configuration sample hole location is stated to accommodate sample.
5. high-throughput nucleic acid according to claim 4 extracts and purification devices, which is characterized in that the sample hole location includes
First sample hole location and the second sample hole location, when the bracket is located at first position, the shell-like component corresponds to the first sample
Hole location, when the bracket is located at the second position, the shell-like component corresponds to the second sample hole location.
6. high-throughput nucleic acid according to claim 4 extracts and purification devices, which is characterized in that it further include air path system,
To blow to the workbench.
7. high-throughput nucleic acid according to claim 4 extracts and purification devices, which is characterized in that further include heating module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820710302.1U CN208362313U (en) | 2018-05-14 | 2018-05-14 | High-throughput nucleic acid extracts and purification devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820710302.1U CN208362313U (en) | 2018-05-14 | 2018-05-14 | High-throughput nucleic acid extracts and purification devices |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208362313U true CN208362313U (en) | 2019-01-11 |
Family
ID=64934334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820710302.1U Active CN208362313U (en) | 2018-05-14 | 2018-05-14 | High-throughput nucleic acid extracts and purification devices |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208362313U (en) |
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2018
- 2018-05-14 CN CN201820710302.1U patent/CN208362313U/en active Active
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