CN208308870U - A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system - Google Patents
A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system Download PDFInfo
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- CN208308870U CN208308870U CN201820536905.4U CN201820536905U CN208308870U CN 208308870 U CN208308870 U CN 208308870U CN 201820536905 U CN201820536905 U CN 201820536905U CN 208308870 U CN208308870 U CN 208308870U
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Abstract
A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system, which is characterized in that including fermentor, continuous condensing crystallizing equipment and centrifuge；The fermentor, continuous condensing crystallizing equipment and centrifuge are sequentially connected, and centrifuge is connected with fermentor；Ceramic membrane is connected between the fermentor and continuous condensing crystallizing equipment, ceramic membrane is connect with fermentor；Compared with prior art, it is fermented by fermentor, continuous condensing crystallizing equipment crystallizes come the clear liquid generated to fermentation, and the crystallization generated by centrifuge to continuous condensing crystallizing equipment and mother liquor separate；The D-Ser of acquisition have higher quality, and generate mother liquor enter in fermentor carry out enzymatic again preparation DL-serine so that resource is more fully utilized.
Description
Technical field
The utility model belongs to chemical production technical field, more particularly, to a kind of Enzyme catalyzed synthesis D-Ser and DL-
Serine joint production process system.
Background technique
A kind of important chiral intermediate of D-Ser, in the application of organic synthesis, new drug research and Peptides Synthesis
Just people is caused more and more to pay close attention to.It is simultaneously also the important centre of some chiral drugs such as seromycin, scheme for lacosamide etc.
Body.With going deep into for D-Ser application study, the demand to D-Ser is also increasing, and just there is an urgent need to have a kind of warp for this
The technique of the efficient production D-Ser of Ji.White crystalline powder is producing eyes, respiratory tract and the irritating effect of skin
It needs to prevent D-Ser and skin and eye contact in the process.DL-serine, as a kind of important biochemical reagents, extensively
Applied in food, feed, medicine, agricultural and cosmetics industry.Due to the limitation of various technical conditions, DL-serine supply
Amount is much smaller than the market demand, therefore DL-serine has been included in " foreign investment industry guidance by national economy and the information-based committee
Catalogue " in, encourage exploitation DL-serine synthesis technology.
Currently, in the prior art, the synthesis of DL-serine can all use hypertoxic raw material cyanogen mainly based on chemical method
Change sodium, mercuric acetate, or starting material is expensive, yield is low, at high cost, shortage industrial value.The preparation of D-Ser is adopted more
It first reacts to form diastereomer isomers with chiral selectors, then hydrolyze using DL-serine as raw material with chemical resolution method
D-Ser is obtained, general yield is not high, causes D-Ser preparation cost high.
Utility model content
The utility model is to provide that a kind of production is simple, and high-efficiency environment friendly is raw to overcome above-mentioned defect in the prior art
Produce high-efficient Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system.
In order to reach the goals above, the technical scheme adopted by the utility model is a kind of Enzyme catalyzed synthesis D-Ser
With DL-serine joint production process system, which is characterized in that including fermentor, continuous condensing crystallizing equipment and centrifuge；The hair
Fermentation tank, continuous condensing crystallizing equipment and centrifuge are sequentially connected, and centrifuge is connected with fermentor；The fermentor and continuous
Ceramic membrane is connected between condensing crystallizing equipment, ceramic membrane is connect with fermentor.
As a kind of preferred embodiment of the utility model, it was additionally provided between the fermentor and continuous condensing crystallizing equipment
Device is filtered, filter device is connect with ceramic membrane.
As a kind of preferred embodiment of the utility model, the filter device includes nanofiltration membrane and filter, nanofiltration membrane with
Ceramic membrane connection, filter are connect with continuous condensing crystallizing equipment.
As a kind of preferred embodiment of the utility model, the ceramic membrane is connected with enzyme storage tank, and enzyme storage tank is connect with fermentor.
As a kind of preferred embodiment of the utility model, the discharge port of the ceramic membrane is connect with the feed inlet of enzyme storage tank,
The discharge port of enzyme storage tank and the feed inlet of fermentor connect.
As a kind of preferred embodiment of the utility model, the continuous condensing crystallizing equipment includes continuous circulating tank and material
Pump, continuous circulating tank are connected with product pump.
As a kind of preferred embodiment of the utility model, the continuous circulating tank is connected to the filter, product pump and centrifugation
Machine connection.
As a kind of preferred embodiment of the utility model, the discharge port of the centrifuge and the feed inlet of fermentor are connected.
As a kind of preferred embodiment of the utility model, another discharge port of the centrifuge is connected with double cone dryer.
As a kind of preferred embodiment of the utility model, the fermentor is fermentor or enzymatic tank.
The beneficial effects of the utility model are: being fermented by fermentor, continuous condensing crystallizing equipment to produce fermentation
Raw clear liquid is crystallized, and the crystallization generated by centrifuge to continuous condensing crystallizing equipment and mother liquor separate；So that
Obtained crystallization have higher precision, and generate mother liquor be again introduced into fermentor carry out conversion DL-serine is made, significantly
The waste of D-Ser is avoided, so that resource is more fully utilized.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Appended drawing reference in figure: fermentor 1, ceramic membrane 2, nanofiltration membrane 3, filter 4, enzyme storage tank 5, continuous condensing crystallizing equipment
6, continuous circulating tank 6-1, product pump 6-2, centrifuge 7, double cone dryer 8.
Specific embodiment
It elaborates with reference to the accompanying drawing to the utility model embodiment.
As shown in Figure 1, a kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system, including fermentor 1,
Continuous condensing crystallizing equipment 6 and centrifuge 7；Fermentor 1, continuous condensing crystallizing equipment 6 and centrifuge 7 are sequentially connected, and are centrifuged
Machine 7 is connected with fermentor 1；Ceramic membrane 2, ceramic membrane 2 and fermentor 1 are connected between fermentor 1 and continuous condensing crystallizing equipment 6
Connection.
It is fermented by fermentor 1 and generates liquid containing enzyme fermentation, after ceramic membrane 2 cleans enzyme solution, it is cold to be transferred to enzyme storage tank 5
Hiding saves, and the enzyme cleaned is again transferred to fermentor 1 and carries out enzymatic, ceramic membrane 2 is filtered retention to enzymatic liquid
Clear liquid is added in continuous condensing crystallizing equipment 6 carries out condensing crystallizing later, has when in continuous condensing crystallizing equipment 6 by enzyme bacterium mud
Material is directly entered in centrifuge 7 when a large amount of crystal occur, and when centrifugation is eluted with a small amount of water, thus in centrifuge 7
Crystallization and mother liquor are separated under effect.
It is poured into fermentor 1 after the generated multiple batches of mixing of mother liquor, amino acid racemase is added and carries out enzymatic generation
DL-serine, generated DL-serine enters in ceramic membrane 2 plus water is cleaned and become clear liquid, and clear liquid is added and is connected
Carry out condensing crystallizing in continuous condensing crystallizing equipment 6, when thering are a large amount of crystal to occur in continuous condensing crystallizing equipment 6 material directly into
Enter in centrifuge 7, when centrifugation is eluted with a small amount of water, to be divided crystallization and mother liquor under the action of centrifuge 7
From the DL-serine that generated crystal is exactly.
Filter device is additionally provided between fermentor 1 and continuous condensing crystallizing equipment 6, filter device is connect with ceramic membrane 2, mistake
Filtering device includes nanofiltration membrane 3 and filter 4, and nanofiltration membrane 3 is connect with ceramic membrane 2, and filter 4 and continuous condensing crystallizing equipment 6 connect
It connects.
The discharge port of nanofiltration membrane 3 is connect with the feed inlet of filter 4, and nanofiltration membrane 3 enters nanofiltration for the decoloration that cleans, clear liquid
In the salt of retention part and pigment after film 3, clear liquid obtains further impurity elimination, ceramic membrane 2 under the action of filter 4 again later
Generated clear liquid, by double filtration, is had under the action of nanofiltration membrane 3 and filter 4 into continuous condensing crystallizing equipment 6
There is the serine of higher concentration.
Ceramic membrane 2 is connected with enzyme storage tank 5, and enzyme storage tank 5 is connect with fermentor 1, the discharge port of ceramic membrane 2 and enzyme storage tank 5 into
Material mouth connection, the discharge port of enzyme storage tank 5 are connect with the feed inlet of fermentor 1, and fermentor 1 carries out the fermentation of the cell containing enzyme, contain enzyme
Cell is cleaned into ceramic membrane 2, and the cell containing enzyme after cleaning is pumped into enzyme storage tank 5 and is saved, and enzyme storage tank 5 can be by enzyme
It squeezes into fermentor 1 and is catalyzed.
Continuous condensing crystallizing equipment 6 includes continuous circulating tank 6-1 and product pump 6-2, continuous circulating tank 6-1 and product pump 6-
2 are connected, and continuous circulating tank 6-1 is connect with filter 4, and product pump 6-2 is connect with centrifuge 7, and clear liquid is in continuous circulating tank 6-1
Middle carry out condensing crystallizing, when there are a large amount of crystal, material enters centrifuge 7 under the action of product pump 6-2.
The discharge port of centrifuge 7 is connect with the feed inlet of fermentor 1, and it is dry that another discharge port of centrifuge 7 is connected with bipyramid
Dry device 8, double cone dryer 8 is for drying obtained crystal, being mixed, and drying is laggard under the action of double cone dryer 8
Row packaging.
This technology is using formaldehyde and glycine as substrate, under the catalytic action of D-Thr aldolase and coenzyme, by one
Molecule formaldehyde and a molecule glycine are converted into the D-Ser of a molecule single configuration, are then catalyzed and make in amino acid racemase
With lower racemization, DL-serine is generated.The technology is with easy to operate, with short production cycle, production cost is low, high income, environmental protection
Pressure is small, is suitble to the advantages that large-scale industrial production.
The foregoing description of the disclosed embodiments can be realized professional and technical personnel in the field or using originally practical new
Type.Various modifications to these embodiments will be readily apparent to those skilled in the art, and determine herein
The General Principle of justice can be realized in other embodiments without departing from the spirit or scope of the present utility model；Cause
This, the present invention will not be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The widest scope consistent with features of novelty.
Although appended drawing reference in figure is used more herein: fermentor 1, ceramic membrane 2, nanofiltration membrane 3, filter 4, enzyme storage
Tank 5, continuous condensing crystallizing equipment 6, continuous circulating tank 6-1, product pump 6-2, centrifuge 7, the equal terms of double cone dryer 8, but simultaneously
A possibility that being not excluded for using other terms；The use of these items is only for be more convenient to describe and explain the utility model
Essence；It is contrary to the spirit of the present invention to interpret them as any one of the additional limitations.
Claims (10)
1. a kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system, which is characterized in that including fermentor (1),
Continuous condensing crystallizing equipment (6) and centrifuge (7)；The fermentor (1), continuous condensing crystallizing equipment (6) and centrifuge (7) according to
Secondary connection, and centrifuge (7) is connected with fermentor (1)；Connect between the fermentor (1) and continuous condensing crystallizing equipment (6)
Have ceramic membrane (2), ceramic membrane (2) is connect with fermentor (1).
2. a kind of Enzyme catalyzed synthesis D-Ser according to claim 1 and DL-serine joint production process system, feature
It is, is additionally provided with filter device between the fermentor (1) and continuous condensing crystallizing equipment (6), filter device and ceramic membrane (2)
Connection.
3. a kind of Enzyme catalyzed synthesis D-Ser according to claim 2 and DL-serine joint production process system, feature
It is, the filter device includes nanofiltration membrane (3) and filter (4), and nanofiltration membrane (3) is connect with ceramic membrane (2), filter (4)
It is connect with continuous condensing crystallizing equipment (6).
4. a kind of Enzyme catalyzed synthesis D-Ser according to claim 3 and DL-serine joint production process system, feature
It is, the ceramic membrane (2) is connected with enzyme storage tank (5), and enzyme storage tank (5) is connect with fermentor (1).
5. a kind of Enzyme catalyzed synthesis D-Ser according to claim 4 and DL-serine joint production process system, feature
It is, the discharge port of the ceramic membrane (2) is connect with the feed inlet of enzyme storage tank (5), the discharge port and fermentor of enzyme storage tank (5)
(1) feed inlet connection.
6. a kind of Enzyme catalyzed synthesis D-Ser according to claim 1 and DL-serine joint production process system, feature
It is, the continuous condensing crystallizing equipment (6) includes continuous circulating tank (6-1) and product pump (6-2), continuous circulating tank (6-1)
It is connected with product pump (6-2).
7. a kind of Enzyme catalyzed synthesis D-Ser according to claim 6 and DL-serine joint production process system, feature
It is, the continuous circulating tank (6-1) connect with filter (4), and product pump (6-2) is connect with centrifuge (7).
8. a kind of Enzyme catalyzed synthesis D-Ser according to claim 7 and DL-serine joint production process system, feature
It is, the discharge port of the centrifuge (7) is connect with the feed inlet of fermentor (1).
9. a kind of Enzyme catalyzed synthesis D-Ser according to claim 8 and DL-serine joint production process system, feature
It is, another discharge port of the centrifuge (7) is connected with double cone dryer (8).
10. a kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system according to claim 1, feature
It is, the fermentor (1) is fermentor or enzymatic tank.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820536905.4U CN208308870U (en) | 2018-04-16 | 2018-04-16 | A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system |
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| CN201820536905.4U CN208308870U (en) | 2018-04-16 | 2018-04-16 | A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110373440A (en) * | 2019-07-23 | 2019-10-25 | 长兴制药股份有限公司 | The method of one pot of enzyme process preparation DL-serine |
| CN114807260A (en) * | 2022-06-08 | 2022-07-29 | 长兴制药股份有限公司 | Enzymatic synthesis mode of D-tryptophan |
-
2018
- 2018-04-16 CN CN201820536905.4U patent/CN208308870U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110373440A (en) * | 2019-07-23 | 2019-10-25 | 长兴制药股份有限公司 | The method of one pot of enzyme process preparation DL-serine |
| CN110373440B (en) * | 2019-07-23 | 2022-03-01 | 长兴制药股份有限公司 | Method for preparing DL-serine by one-pot enzyme method |
| CN114807260A (en) * | 2022-06-08 | 2022-07-29 | 长兴制药股份有限公司 | Enzymatic synthesis mode of D-tryptophan |
| CN114807260B (en) * | 2022-06-08 | 2024-02-09 | 长兴制药股份有限公司 | Enzymatic synthesis mode of D-tryptophan |
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